LOCUS       DAZKCE010000001        80104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000001 DAZKCE010000000
VERSION     DAZKCE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.001"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..919)
                     /locus_tag="ACG8KQ_000001"
     CDS             complement(179..919)
                     /locus_tag="ACG8KQ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9102981.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1049..1996)
                     /locus_tag="ACG8KQ_000002"
     CDS             complement(1049..1996)
                     /locus_tag="ACG8KQ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9102982.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2125..2889)
                     /locus_tag="ACG8KQ_000003"
     CDS             complement(2125..2889)
                     /locus_tag="ACG8KQ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9102983.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2893..3282)
                     /locus_tag="ACG8KQ_000004"
     CDS             complement(2893..3282)
                     /locus_tag="ACG8KQ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9102984.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3417..3959
                     /locus_tag="ACG8KQ_000005"
     CDS             3417..3959
                     /locus_tag="ACG8KQ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9102985.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4107..4739
                     /locus_tag="ACG8KQ_000006"
     CDS             4107..4739
                     /locus_tag="ACG8KQ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9102986.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4873..4998)
                     /locus_tag="ACG8KQ_000007"
     CDS             complement(4873..4998)
                     /locus_tag="ACG8KQ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9102987.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5132..6025)
                     /locus_tag="ACG8KQ_000008"
     CDS             complement(5132..6025)
                     /locus_tag="ACG8KQ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9102988.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6207..7133)
                     /locus_tag="ACG8KQ_000009"
     CDS             complement(6207..7133)
                     /locus_tag="ACG8KQ_000009"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9102989.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7218..7979)
                     /gene="pflA"
                     /locus_tag="ACG8KQ_000010"
     CDS             complement(7218..7979)
                     /gene="pflA"
                     /locus_tag="ACG8KQ_000010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9102990.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8223..10454)
                     /gene="pflB"
                     /locus_tag="ACG8KQ_000011"
     CDS             complement(8223..10454)
                     /gene="pflB"
                     /locus_tag="ACG8KQ_000011"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9102991.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10731..13181)
                     /gene="parC"
                     /locus_tag="ACG8KQ_000012"
     CDS             complement(10731..13181)
                     /gene="parC"
                     /locus_tag="ACG8KQ_000012"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9102992.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13193..15238)
                     /gene="parE"
                     /locus_tag="ACG8KQ_000013"
     CDS             complement(13193..15238)
                     /gene="parE"
                     /locus_tag="ACG8KQ_000013"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9102993.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15404..15838)
                     /locus_tag="ACG8KQ_000014"
     CDS             complement(15404..15838)
                     /locus_tag="ACG8KQ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9102994.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15965..16603
                     /gene="plsY"
                     /locus_tag="ACG8KQ_000015"
     CDS             15965..16603
                     /gene="plsY"
                     /locus_tag="ACG8KQ_000015"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9102995.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16735..17610)
                     /locus_tag="ACG8KQ_000016"
     CDS             complement(16735..17610)
                     /locus_tag="ACG8KQ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9102996.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17692..18483)
                     /gene="codY"
                     /locus_tag="ACG8KQ_000017"
     CDS             complement(17692..18483)
                     /gene="codY"
                     /locus_tag="ACG8KQ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9102997.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18501..19901)
                     /gene="hslU"
                     /locus_tag="ACG8KQ_000018"
     CDS             complement(18501..19901)
                     /gene="hslU"
                     /locus_tag="ACG8KQ_000018"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9102998.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19915..20463)
                     /gene="hslV"
                     /locus_tag="ACG8KQ_000019"
     CDS             complement(19915..20463)
                     /gene="hslV"
                     /locus_tag="ACG8KQ_000019"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9102999.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20500..21405)
                     /gene="xerC"
                     /locus_tag="ACG8KQ_000020"
     CDS             complement(20500..21405)
                     /gene="xerC"
                     /locus_tag="ACG8KQ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9103000.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21603..22979)
                     /gene="trmFO"
                     /locus_tag="ACG8KQ_000021"
     CDS             complement(21603..22979)
                     /gene="trmFO"
                     /locus_tag="ACG8KQ_000021"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9103001.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22948..25026)
                     /gene="topA"
                     /locus_tag="ACG8KQ_000022"
     CDS             complement(22948..25026)
                     /gene="topA"
                     /locus_tag="ACG8KQ_000022"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9103002.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25148..26005)
                     /gene="dprA"
                     /locus_tag="ACG8KQ_000023"
     CDS             complement(25148..26005)
                     /gene="dprA"
                     /locus_tag="ACG8KQ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9103003.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26061..26828)
                     /locus_tag="ACG8KQ_000024"
     CDS             complement(26061..26828)
                     /locus_tag="ACG8KQ_000024"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9103004.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26821..27681)
                     /gene="ylqF"
                     /locus_tag="ACG8KQ_000025"
     CDS             complement(26821..27681)
                     /gene="ylqF"
                     /locus_tag="ACG8KQ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9103005.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27941..28339
                     /locus_tag="ACG8KQ_000026"
     CDS             27941..28339
                     /locus_tag="ACG8KQ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9103006.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28576..29118
                     /locus_tag="ACG8KQ_000027"
     CDS             28576..29118
                     /locus_tag="ACG8KQ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9103007.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29201..29752)
                     /gene="lepB"
                     /locus_tag="ACG8KQ_000028"
     CDS             complement(29201..29752)
                     /gene="lepB"
                     /locus_tag="ACG8KQ_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9103008.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29904..30131)
                     /locus_tag="ACG8KQ_000029"
     CDS             complement(29904..30131)
                     /locus_tag="ACG8KQ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9103009.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30128..30646)
                     /gene="msrA"
                     /locus_tag="ACG8KQ_000030"
     CDS             complement(30128..30646)
                     /gene="msrA"
                     /locus_tag="ACG8KQ_000030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9103010.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30666..31379)
                     /locus_tag="ACG8KQ_000031"
     CDS             complement(30666..31379)
                     /locus_tag="ACG8KQ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9103011.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31382..32278)
                     /locus_tag="ACG8KQ_000032"
     CDS             complement(31382..32278)
                     /locus_tag="ACG8KQ_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9103012.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32436..33278)
                     /locus_tag="ACG8KQ_000033"
     CDS             complement(32436..33278)
                     /locus_tag="ACG8KQ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9103013.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33410..34063
                     /locus_tag="ACG8KQ_000034"
     CDS             33410..34063
                     /locus_tag="ACG8KQ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9103014.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34126..34653)
                     /locus_tag="ACG8KQ_000035"
     CDS             complement(34126..34653)
                     /locus_tag="ACG8KQ_000035"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9103015.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34673..35620)
                     /locus_tag="ACG8KQ_000036"
     CDS             complement(34673..35620)
                     /locus_tag="ACG8KQ_000036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9103016.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35632..37515)
                     /locus_tag="ACG8KQ_000037"
     CDS             complement(35632..37515)
                     /locus_tag="ACG8KQ_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9103017.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37678..39756)
                     /locus_tag="ACG8KQ_000038"
     CDS             complement(37678..39756)
                     /locus_tag="ACG8KQ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9103018.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39769..40044)
                     /locus_tag="ACG8KQ_000039"
     CDS             complement(39769..40044)
                     /locus_tag="ACG8KQ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103019.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40406..40861)
                     /locus_tag="ACG8KQ_000040"
     CDS             complement(40406..40861)
                     /locus_tag="ACG8KQ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103020.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41015..42223)
                     /locus_tag="ACG8KQ_000041"
     CDS             complement(41015..42223)
                     /locus_tag="ACG8KQ_000041"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9103021.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42335..42667)
                     /locus_tag="ACG8KQ_000042"
     CDS             complement(42335..42667)
                     /locus_tag="ACG8KQ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9103022.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42815..43690
                     /locus_tag="ACG8KQ_000043"
     CDS             42815..43690
                     /locus_tag="ACG8KQ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9103023.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43772..44353)
                     /locus_tag="ACG8KQ_000044"
     CDS             complement(43772..44353)
                     /locus_tag="ACG8KQ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9103024.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44359..45618)
                     /locus_tag="ACG8KQ_000045"
     CDS             complement(44359..45618)
                     /locus_tag="ACG8KQ_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9103025.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45620..46666)
                     /locus_tag="ACG8KQ_000046"
     CDS             complement(45620..46666)
                     /locus_tag="ACG8KQ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103026.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46901..47176)
                     /locus_tag="ACG8KQ_000047"
     CDS             complement(46901..47176)
                     /locus_tag="ACG8KQ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9103027.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47419..48729)
                     /gene="der"
                     /locus_tag="ACG8KQ_000048"
     CDS             complement(47419..48729)
                     /gene="der"
                     /locus_tag="ACG8KQ_000048"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9103028.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48911..50140)
                     /gene="rpsA"
                     /locus_tag="ACG8KQ_000049"
     CDS             complement(48911..50140)
                     /gene="rpsA"
                     /locus_tag="ACG8KQ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9103029.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50275..50955)
                     /gene="cmk"
                     /locus_tag="ACG8KQ_000050"
     CDS             complement(50275..50955)
                     /gene="cmk"
                     /locus_tag="ACG8KQ_000050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9103030.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51004..51639)
                     /locus_tag="ACG8KQ_000051"
     CDS             complement(51004..51639)
                     /locus_tag="ACG8KQ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9103031.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51705..53108)
                     /locus_tag="ACG8KQ_000052"
     CDS             complement(51705..53108)
                     /locus_tag="ACG8KQ_000052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9103032.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53095..54126)
                     /locus_tag="ACG8KQ_000053"
     CDS             complement(53095..54126)
                     /locus_tag="ACG8KQ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103033.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54157..54378
                     /locus_tag="ACG8KQ_000054"
     CDS             54157..54378
                     /locus_tag="ACG8KQ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9103034.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54543..55280)
                     /locus_tag="ACG8KQ_000055"
     CDS             complement(54543..55280)
                     /locus_tag="ACG8KQ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP9103035.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55433..56773)
                     /locus_tag="ACG8KQ_000056"
     CDS             complement(55433..56773)
                     /locus_tag="ACG8KQ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9103036.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57010..58533)
                     /locus_tag="ACG8KQ_000057"
     CDS             complement(57010..58533)
                     /locus_tag="ACG8KQ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9103037.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58911..59522)
                     /locus_tag="ACG8KQ_000058"
     CDS             complement(58911..59522)
                     /locus_tag="ACG8KQ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9103038.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59645..59770)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59852..60568)
                     /locus_tag="ACG8KQ_000059"
     CDS             complement(59852..60568)
                     /locus_tag="ACG8KQ_000059"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9103039.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60574..61143)
                     /gene="scpB"
                     /locus_tag="ACG8KQ_000060"
     CDS             complement(60574..61143)
                     /gene="scpB"
                     /locus_tag="ACG8KQ_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9103040.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61127..61897)
                     /locus_tag="ACG8KQ_000061"
     CDS             complement(61127..61897)
                     /locus_tag="ACG8KQ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9103041.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61894..62265)
                     /locus_tag="ACG8KQ_000062"
     CDS             complement(61894..62265)
                     /locus_tag="ACG8KQ_000062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9103042.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62284..63171)
                     /gene="xerD"
                     /locus_tag="ACG8KQ_000063"
     CDS             complement(62284..63171)
                     /gene="xerD"
                     /locus_tag="ACG8KQ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9103043.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63267..63740)
                     /locus_tag="ACG8KQ_000064"
     CDS             complement(63267..63740)
                     /locus_tag="ACG8KQ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9103044.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63845..64708)
                     /locus_tag="ACG8KQ_000065"
     CDS             complement(63845..64708)
                     /locus_tag="ACG8KQ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9103045.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64904..67003)
                     /locus_tag="ACG8KQ_000066"
     CDS             complement(64904..67003)
                     /locus_tag="ACG8KQ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9103046.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67223..68332)
                     /gene="rpoD"
                     /locus_tag="ACG8KQ_000067"
     CDS             complement(67223..68332)
                     /gene="rpoD"
                     /locus_tag="ACG8KQ_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9103047.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68356..70197)
                     /gene="dnaG"
                     /locus_tag="ACG8KQ_000068"
     CDS             complement(68356..70197)
                     /gene="dnaG"
                     /locus_tag="ACG8KQ_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9103048.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70379..72694)
                     /locus_tag="ACG8KQ_000069"
     CDS             complement(70379..72694)
                     /locus_tag="ACG8KQ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9103049.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72879..73739)
                     /locus_tag="ACG8KQ_000070"
     CDS             complement(72879..73739)
                     /locus_tag="ACG8KQ_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9103050.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73798..74430)
                     /locus_tag="ACG8KQ_000071"
                     /pseudo
     CDS             complement(73798..74430)
                     /locus_tag="ACG8KQ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            74597..75358
                     /locus_tag="ACG8KQ_000072"
     CDS             74597..75358
                     /locus_tag="ACG8KQ_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9103051.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75384..77975
                     /gene="mprF"
                     /locus_tag="ACG8KQ_000073"
     CDS             75384..77975
                     /gene="mprF"
                     /locus_tag="ACG8KQ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9103052.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78163..79350)
                     /gene="tuf"
                     /locus_tag="ACG8KQ_000074"
     CDS             complement(78163..79350)
                     /gene="tuf"
                     /locus_tag="ACG8KQ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9103053.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            79718..80074
                     /locus_tag="ACG8KQ_000075"
     CDS             79718..80074
                     /locus_tag="ACG8KQ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9103054.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        21962 a        16841 c        13739 g        27562 t
ORIGIN      
        1 ctgcgaaaat agtttgtttt tttattttgc cattattttc ttacttattg ggagaaaaaa
       61 ttcgcttaaa ctcaaaaaaa tttgaggata cgatataaac tttcggatag agtataaaat
      121 aaggaaacgc cgcagcgttt cctttccaac tgtagccgaa tataaatttg tatatcactt
      181 atttaaaata gctattttct cccaaagagt ttccttgatc atcgatcaat ccttgtaatg
      241 agcgttctaa gataccatgg ctttttttcg attttttttc ttctgactgc tttgtttcct
      301 ctaacgtttt tgttcgattg aatgttcgta cagacggctc accatttctt ttttcttgat
      361 acggcgtcgg ttctgtatcg aacaacagat aagagtcatc cgtttttttc atcgattcga
      421 ttaaatgatc ctcagaaatt ttgctttctc tagggtctgg gataatcgat gcgggtacat
      481 atttaggaac aaaataagaa cgctcccgca ccttcgtgtc aacagatgcg tttcgtgtag
      541 tatgcttgga ctctgtcctt gtttcacttt gtctgacaga acgagaggaa tcaccaaaaa
      601 gtttttttcg tcctgtcgat gtgatttctg attggtaatg gccggtataa tccggtaatt
      661 ttcctttatg cttttccaaa ttttccttac gcggagctga cttcaatcca tttgttgcac
      721 gacgcttgcg acggattttc tcggtgttcg tgttcgtgaa atgaaattct ttcatctgtt
      781 caaacgggga ctgattctgt tcagtcaaaa gatcttcttg tccgtcaaat aatactcgct
      841 tcttgtcgtt ggttaatttt acgcctaact catcatcgta cgcaggaaaa cggaaatgtt
      901 ttcgtctgat ttctttcatt caaaccatcc ttctcaaaaa tggaactttt ctcaataata
      961 gcacagattc attgttttca gtagcaatgc gctgattatt ttaagaaaat agcgatgaat
     1021 ttcataaata tatatatagt aataagactt aattcgttgt ctctctgata actagttcag
     1081 gaaaaaaacg acgataacct tgactcgccc ctttttcttc tatttttttt aacaacattt
     1141 ctccacatac ttcacccata cgtataatat cttgcttgat cgttgtcaat ttaggtgaac
     1201 tgatttggtc taaaaatacc ccatcaaatc caatcacacc aaattcttcg ggcaagtgtc
     1261 ctccagcatt tataatccct cttactgctc ctagtgctag ccgatctgaa ctacatacaa
     1321 aacaagtttt atctgtaaaa tctgcccagt tttctgtcat atatctttct acttctgtcg
     1381 aatgatttgg aaaccgcaag attttactag gaagatcaac ttccttcatc acagataaat
     1441 aaccagtttc ccttgaaatt tcaaaaggct cgtccacttc gataccgata taaataatcg
     1501 tttgataatc tctttctaag gcatattgcg ttgcttttgc tgtaccatat tcattatcgc
     1561 tgtctacata atcatatcct tcagaatttt ccccaaacaa gacaactggt ttttccaaac
     1621 ggtcgatcca atcgaagtct ttttttctga cacctgtgat gatgtatcca tcacaagaac
     1681 cgacgtcgaa ggcatcattt gtaactaatt ggagagaata atgcgcttta ccgatccgtt
     1741 tagcgattcc catcagcaaa ttcatgtaat agggctcagt tgtgtcgatt tcttccagta
     1801 taaataattt gatgatctgc gaccgattcg tgacaagtgc ttttgcagca atatttggac
     1861 ggtaatccag ttcagccatt gctgagaaaa ctaactcttt caattcgtca gtcacctgtt
     1921 ccggatgatt gatgactcga gagaccgtca tttttgaaac attggcacgt tttgcaacat
     1981 ctgctaaagt gggcatgctc tcttcctttc taacatgttg tcattcttac tatcaattat
     2041 accaaatttt cctaacagag tttattatag cccaaaataa aaaacggaaa atcctctatc
     2101 gtcatgagtg attttccgtt tcaattattt atactttttt atcaaatttt ttgctaaaaa
     2161 agtggaagct tcttccatta gtggtatttg taccatatgt cgctgtgttt ttgaggaacg
     2221 aaaaacaatt tcttttccgt aagacttatg ccgatttttt tctacaaatc gttccgtttg
     2281 tatataaggg attttttcgt cagcttgtcc atgccaaaag aacagaggac gtccagctaa
     2341 cttttccggc tgcaatgaca aatcatagct cttaacccat gaaatcaaat caaaatagtc
     2401 ttttggtacg acataattat attccgatgc gcgccttacg atttcttttc catattccaa
     2461 aggtgcaggg gaacccatca aacacacgcc cgctactata tgaggatgtt tagccaacaa
     2521 ggcgcatgtt gtcattcccc ccatcgaaac cccgccaact gcaagctgat catgtagaag
     2581 acctcttttt tctaaatgct tgataagtgt gtcaaattca aacagatttc cgtagatact
     2641 atcccaaaat gtataggatg gaatagaaga aaccggctgt ttgcgctccc catgattcat
     2701 cgcatctggc aggatcacac gaatgccctg ctgcgcaatt tttcttcctt gcgtcaaaac
     2761 taactcctta cttgtctgcc atccgtgata atagatgaca acgggaatac gttcctctct
     2821 taattctttc agaacaactt ctaacactgg aatatgatgg acagaacgtt tcaatataat
     2881 tggattcaat tgttactcac ctattttcat taaatttcct gatcgccata gcaaccatgc
     2941 tccaacagtt gtctggaaca ggttcagtgt accaaataat ataaatgcag gactgaatgg
     3001 ctcgggaaat aagcacagag aacaaagtcc caccactcct aatgctagcg aaaggagtgg
     3061 ataaatagaa aaattcaaaa aactggcaac gctaccgaaa atattaccaa taaacagtat
     3121 caaaaagaaa aaaacgctta aaattaaacc atctgtcagc gacatctgaa aaacaatgtt
     3181 ctctacctca attggctgtt gaatcgaata gacacctaag tatatactga gaaggagact
     3241 tagcacgtgg ctggcacaaa acacacgatt gaccgttttc attttaatca cctaacctat
     3301 ctttataatt ctattataat cattttatca tttttttgta tgtataaagt ttcaattttt
     3361 cttcacttac gatttttttt gatattgttg ttatataaat gaaaggagtt ttacaaatga
     3421 ttgatccaaa aatgttgggg atgatcttcc tcattaattt tgcttatatc actctgaaca
     3481 cgttacgttt catgttgaca atgaaaggct atcgactgat tgctccactt gtcagtatgg
     3541 tcgagatcac gatttatatc gtcggcttat cccttgtttt agatcgttta gataatccac
     3601 tgaatttatt tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga attttgattg
     3661 aagacaaatt ggcattagga tacatcatgg tcacaacgat tttgccagca aatagcgcag
     3721 aagataaaag tttgccgggt atactgagag aacacggtta tggtgtgaca cagacttctg
     3781 caatggggct ggaaggtgag cggctagttc tcgaaatact ttctcctaga aaaagcgaac
     3841 gtgaactgta tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt tcttatgagc
     3901 caaaatatat ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa ggaaaataac
     3961 tcacaaactt aaaaactgtc taatatctaa attaaatttt aaatgttaga cagtttttat
     4021 ttattatttt gattaaaaaa catcgacata tactcatcaa tttgttatta tactttatat
     4081 acttatcaat ttaatttgga gatattatgg aaattcaacg tagaaccact aattttttac
     4141 atttaggctc tttttttgca actttgacga tcttattttt tctatgttat tcatacctta
     4201 agacctcaca tgcgccttct agtgcattag gctggttgct tttggatcat tctgatatca
     4261 cagaagccga agaaaatccg ttttttttga ttttgtcttc tctttgcggc ttactgtttt
     4321 ctgtttattt cggtgcctat ttttttcatc gacatcctgg tacgttaaac gaagcttcaa
     4381 gcgtattgat tgcatttaaa acgttttctt ttttagcaat tcttttttat tcgtttaatc
     4441 acaatacgct tttcttcttt atcatgaatg gtttagtcgt gattttatcc atttttactt
     4501 attttatttt ttcactcatt tttagccaat tgaaatgtcc gctcttcttt cgattagttc
     4561 ctgtcacctg ttttatttac agcttgtttt ccttttttgt ccttgcttta atgccactga
     4621 ccaacccttc gacgatccaa gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc
     4681 taggtgtatt tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt gaagcctgac
     4741 tattacgact tcataaatgc ttttttagtt ttaaacactt caatctaaaa gaataaccca
     4801 agattggata aattattaat gactcgccat tcgtttctta gtgattctgg attatcaaat
     4861 ggacttccta atctaatcag ctagttcttc ccataaaatg ccatacttga ctgaatcata
     4921 atattgatta tttaaaaatc taacttttct tatcactact tcctttgtca ttccacattt
     4981 ctcaccattt ttttgcattc ctttgtttcc agaccatgta gtgaatctaa taggtggtag
     5041 ataggcaaaa tactcaaata attcttttaa ccataaagga agagaaaggc tcctcctgca
     5101 agagtcagta taatttttgc ttactgctct cttaggtttt actcactaaa actgttccat
     5161 attctttgat ataagaaaat gcatgcggta taaatttctt gctcagctca gaaaagattt
     5221 gtcgtttaag aaaggctatt tcctccattg tcaatgaagg atccaatgtt ggtaattcaa
     5281 tctcaaaatt gtttttttcg acaataggta attctaatgt gaatgattct tcttccttta
     5341 tagtattcgt cacaagtaga gactgatgga aaatactagg ccggcgattt ttatatttat
     5401 tcttacgtat cctgtcaacg ataacttcta tttgatcaaa aaagtacgat tcatttacgt
     5461 ctccaataag ttcggctaat tctttgaatg ccttcgttac aggaagctga ttcttctgta
     5521 caaaaaagta attagccaaa gtaagcggga agtgttctat agaggaaagt gtgataaaac
     5581 ggtacttctc atttcctgca gttgtcctca gcaattctgt ctctcggctg tttcttactc
     5641 cataaaaata cgcttctttc cgttcatctt cgaaacaata cgcccaaata acttctccca
     5701 tcgctaattt ttgttcatca acagagagtc tcttcaattg ttcagctatg gtttcagcag
     5761 ccgaagtggc ttgttgcaag taatctttag gatcaaatat tggaaagttc aaatgatatc
     5821 ttcgttcttc cctcttgatt aatcctgcat cgatcaataa atcaagaaaa tgggaaaatt
     5881 tattttgcga aaattctttt ttcaattctc ttagaatcgt ttcttgttct cgatgttcat
     5941 atagataatc ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga taaaagtttt
     6001 ccttattttt tgaataatag ttcatatatg ttctcttttc tatccgtttt attttactta
     6061 aaaggctttt acgtaaaaag gaggaagaac aagagtcata attgactgtt attctcctcc
     6121 atttcttcgg cctttagtat gttctatgtt tctttttata tcaatcgctt atccattcat
     6181 tttgaatgct tcatactacc atttgattag ctaaatacag ccgtcaattg aggtacgact
     6241 tgttttttac gtgaaacgac acctttaagc aaagcacggt tgttggtcaa agttgtttgg
     6301 aaagcttctt ctactttttc ttttccttta ccagcaacaa gtaattctga atcactgtct
     6361 aagatgtttg tgacaactaa tacgaacaga tcgtagccat ttgctttcat ttcatcttcc
     6421 attgctgctt ctagctctgc ttgtcgatcc atcacttcat taaggtccac tgtattgact
     6481 tgagcgatac gaacgttgga actgcccatt gggaaacttt ttgcatccaa atcaagaaga
     6541 accgcagctg atttatcact tagatttgtt cctgctttca acatttcaag tccataagtg
     6601 tttagatcga cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc ttcaggcgta
     6661 catgttggtg atttgaacaa caatgtatct gaaatgatag ctgagagcat gattcctgcg
     6721 atttctttag gaattgttac ttggttttct ttatatagtt ttaaaataat cgtacttgta
     6781 catccaactg gttctgcacg atagtacaat ggattagctg tttcaaagtt tgcaatacga
     6841 tggtggtcta ctacagctaa aatatttact tcgctgatat ctgaaatact ttgttggaat
     6901 tcattatgat ccacaagcat gacatctgca ttttctgctt tagctgactc tactactcgt
     6961 ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt cttcattagg tgtacctaga
     7021 gcaactgctt ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc aatggctgcc
     7081 ccgattgcat ctgtatctgg attttggtga ccaaaaacta aaatttttga catgtcattc
     7141 gctcctttgt gatttctcct atttaataat agcaaaaaaa cggaaagaag caatacttat
     7201 cttctttccg atgcgaccta tcgcgttaaa tattttgtat actcttctgt atgaagcaat
     7261 cgtttagcat tttcgacacg ttcttttgta ggtggttcga tcccttctaa tggatatttc
     7321 aaaccaagtg tttcccactt gtattttccc atcgtatgat aaggaagaat ctctactctg
     7381 tctacatttg acaaggtttg aataaagttg tctagtcgga tcaaatattc atcataatca
     7441 cttcgctcag gaactagtac atggcgaatc caaaccggtt tgttgatttt tgacaaatat
     7501 tgagccattt ctaaaatatt ttcattgtcc caatgcgtta gttttttatg ctcttcatta
     7561 tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact tcatcaattc ttgaaaacgt
     7621 gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg tatccaatgt agtatgcatc
     7681 ttccgctctt ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa tagaggttct
     7741 ccaccactaa cagtaatacc acctttatct ccccagtaag gtctgaagcg ttctgcttta
     7801 tctaaaagtt catctgctgt gtattctttc cctccgccaa tattccaagt atctggattg
     7861 tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg cagtccaggt
     7921 ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt ttcttccatt
     7981 taagttcaca tcttttcttt gatttcattt taagtacaag ttagctgtcg aagcgaagct
     8041 ttctgaacaa aatttttcgc aattgccgct tcgttatgtg taaaatttac agggacggaa
     8101 catacaagtc ctgcccctgt aaatcgtttc tttcaaataa gcatactgat tattcgattt
     8161 tgatgcgatg gctattcaac gtcctttcga cgctaagtcg aaaaactttt ttcacgaact
     8221 ttttacattc tgtcgtgtga agttctcatc aagacatcta attgttgttc tcttgtcaag
     8281 tcacggaatt taactgcata accagatacc cggatcgtca agttcggata tttcattgga
     8341 ttttccattg catcacgcaa taaatcatta ctgaatacat tgacattcaa atgataagct
     8401 ccttttgaga agtatccatc taagacatta cgcaagtttt cgattcttgt atcttgttct
     8461 tttccaagac ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa tgcacagccg
     8521 taattcattt tagctgttga gttcaggcta gctaataacc cactttgttc cgcattgtaa
     8581 cttggatttg ctcctggcgc aagtggtttt ccagcttcac ggccatcagg agtatttcct
     8641 gtatttttac cgtaaacaac atttgaagta atcgtcaata atgaagttgt tggttttgag
     8701 ttgcggtaag ttttatgacg tttgatttgt cccatgaagt attccaagat ccatgaaact
     8761 aattcatctg cacggtcatc atcatttccg tatttaggga agtcgccatc cacttcaaaa
     8821 tcaactgcta ttcctttttc attacggatt gcttttactt ttgcatattt gatcgctgac
     8881 aagctgtctg ccgcatgaga gatcccagca atacctgtag caaatgtgcg ttgcaagtca
     8941 gaatccatca aagccagctg agctgcttca taagaatatt tatcatgcat ataatgaatg
     9001 atgttcaacg tattgacata tagttctgct aaccaatcca tcatattcat gaagttatgt
     9061 ttcacttctt caaagtctag tatttctgat gtgattggac gatattctgg tccaacttgg
     9121 gcgccagtca cttcatcgac tccgccattg atggcatata atagtgtttt agccaagtta
     9181 gcacgagcgc caaagaattg catgtctttt ccgacaactg ttcctgatac acagcaagca
     9241 atcgcacaat catcagaacc ccattgttca cgaagctgat tatcattttc gaattgaatc
     9301 gaagaacttt cgatagcgat tctgctagca tatgttctga atccttctgg aaggtgttca
     9361 gaatacaaaa cagtcaagtt tggttctggt gaaggtccca tatttgttaa tgtatgaagc
     9421 agacggaaat cattctttgt aactaatgaa cgaccatcta atcccatacc tgcaatggat
     9481 aaagtagccc agatagggaa tcctgagaat aattggttat attcaggtgt acgcgcgaat
     9541 ttgaccatac gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc tttttctgtg
     9601 attactccat tatccaaatc acgttggata tagatatcaa ggaatgctga gatacgtccg
     9661 attgacatcg cagcaccatt ttgtgatttg attgctgcta aatatccgaa gtatagccat
     9721 tggacagctt cttttgcatt tgcagctggt ttagaaatat catagccata tttcgcagcc
     9781 atttctttca tttgcgctaa tgcacgatat tgttcagtga tttcttcacg taaacgaatc
     9841 acttcttcac tcattgtatg ataaccagtc atatcatgat cttttttctt ttgttccatc
     9901 aaatagtcga ttccatacaa tgccacacga cgatagtcac cgatgatacg tccgcgaccg
     9961 taagcgtctg gaagccctgt aatgattttg tttttacgag cagcacgcat ttcagcagta
    10021 taagcgtcaa atacaccttg attatgtgtt ttgcgccagt cagtgaaaat atgtgtcaat
    10081 tcatcgtttg gtgtatagcc attgcttttt agcgcattgt tagccatatt gattccgccg
    10141 aacggcatga atgcttgttt caaaggtttg tctgtctgta gaccgacgat tttttccaaa
    10201 tctttatcca aatagcctgc atcgtgtgaa gtaatagttg atacgatttc tgtgtccata
    10261 tcatagacac cgttacgctc gaattggata tcatttaact tttgcaattc atcccataat
    10321 ttttctgttg cttctgttgg gccttccaaa aagctgtcat caccgcgata ttcagtgtag
    10381 ttattttgaa taaagtctct agtgtctacg ccttctttcc atttggtacc tttaaatcca
    10441 ttccattgct ccattgtatg gacctcctaa atttctgttt tatgtaacag tttttacgtg
    10501 attagtataa cacagcaccc tatctttgca agggttttct tgttttttat ttaacaattt
    10561 ttatgtttta tttacatata tttaaatcaa cgttgacata ccaacattta agcattgtga
    10621 acactttcat ctttttttgc aaacgtataa ggatttatta aaaatgaatg aaaaagagac
    10681 gccacctgta ttaatacaga tggcgtctct tcattaaaat actacaacag ttacgactcg
    10741 atgaaaatct caggagcatc gataacttcg atgacttctc cacctttttt ctcatccatc
    10801 gcaaatgatc cattcgatgt tcgatcacct atagggaaat cagttgggtc aatcgttaac
    10861 tgctgccctt tttgattcat caaggttatt tcttttgatc ttccttccga catatagata
    10921 atacggtgcg gatttttttt cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa
    10981 gtttggctga tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt aacgattacg
    11041 atcggcgtat caccttctgc taacacaagc agcccgttga taatcctatc tccatctttc
    11101 aaattcatag acttcactcc agctgccttt gctccaacaa cgggaacatc tgttaaagga
    11161 tagcgcaaac ccattccttg atgcgttact aagaaaacat ctaagcctgg ctgatcttct
    11221 ttcagataca cagcaatcag ttcatcttct tccgccttta gcttcataca gctgagcgga
    11281 cggctcttat aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt gatcaatcca
    11341 ttcttgctga tgaatacaaa tgttttctct gcttgaattt tttgatatgg gaaaacagct
    11401 aaaatcgatt cgtctacaga cagattggag attgtttgag aaatgtgctc accagcatct
    11461 ttccatttca aatcgggtag ttcatggaca gggcggtaga tcacatttgc tttatttgtg
    11521 atcagaagaa catggtccaa tgtactcaat tcacctgagt atagcaagta gtcgccttct
    11581 cgcatcccga tttcttctgg tttggaagca ctataagaac gaaggcttgt tcgcttcaca
    11641 tagccttctc gcgtcacact gacaattacg tcttcttgtg ccactaagac ttgcgtatca
    11701 attttgattt cttcgatttc gtcttcgatt gtcgttagac gagcggaaga ataatttttt
    11761 ttcacttctc gcaattcttt tttcataaca gagaaaagct ctttgtcatt tgaaaggatt
    11821 ttattcaact ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag ctctgtgata
    11881 tccgtgttcg tcaaacggta taattgcaat gtgacaattg cttctgcttg ttcttctgta
    11941 aattgaaaga cgtcgactaa attctttttg gcgtcttttt tgtctttact ttcacgaatc
    12001 gttgcgatta cttcatccag aatcgataaa gctttcatca atccttctac gatatgctgt
    12061 cttttttgag ctttggctaa atcgaattga ctacgttttg ttatgacttg ctttcgatgc
    12121 tcgatataac ttcttaaaat gtctttcagt ccaacttgat gcggagtcat atggtcgata
    12181 gcgaccatat tgaaattgta attgatctgt agctcagtgt ttttgaacaa ataattcaaa
    12241 attccttgag cattggcgtc ttttttcaat tcgacgacaa tttgcagacc tgttctatca
    12301 ctttcatctc gtacttcagc tattccatcg attttcttat tcaaacggat ctcatccatt
    12361 tttttcacca aggtcgcttt atttacttca tatgggattt ctgtgatcac gatttgctgt
    12421 ttattaccct tcattgcctc tatctttgtt ttcgaacgaa gaataacttt tcctcgtcct
    12481 gtttcataag cttttttgat ttcatctttt ccttgcaaga tccctcctgt tggaaagtca
    12541 ggtccaggaa tatattccat caatttttca aggctcgctt gtggatgatc gatcatatag
    12601 attgtgccat cgatcacttc agctaagtta tgagtcggga tttctgttgc atatccagca
    12661 gatatacctg tcgatccatt gacgagaagg tttgggtatc gagctggaag gactgttggt
    12721 tctttttctg tatcatcaaa attccacacg agatcgactg tttctttctc gatatctgct
    12781 agcatctctc cacttaactt ggataatctg gcttcagtat aacgcatcgc agcaggtgga
    12841 tcaccgtcca tactcccgtt attcccgtgc atttcgatca gtacttcccg caacttccag
    12901 tcttggctca tgcggaccat cgcttcataa atactgatgt caccatgagg atgataattc
    12961 cccatgatat ttccgacaga ttttgctgat ttacggaatc ctttgtcata cgtattgcca
    13021 tctttgttca tggcaaataa tatccggcgt tgcactggct tcaatccatc acggatatca
    13081 ggcagtgcac gttcttggat aatatatttg gaataacgtc cgaagcggtc acccatcact
    13141 tcttctaaag ttaattcttg tacttcttgt cgattttcca tcaggtgacc tcctattcta
    13201 cttcaaataa ctgattgttt tcatttttat ctgctcgttc ttcatctaat aaatcattag
    13261 aaactgaagg agagatttca gtatcctctt ttttatctaa gatactgcca tcttcttcga
    13321 gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg ttctactttg tcacccatca
    13381 gtgtggttac tcgacgttca gcttgcgcag catcatcgat acggacgcgg atcaacgtac
    13441 gggaagtcgg atccatcgtt gtttcccaca attgttccgc attcatctct ccaagtcctt
    13501 tatatcgctg cagcatatat cctttgccga cttttttaat cattgctgcc aattcgtcat
    13561 ctgtccacgc atattcaatg acttgcttct ttccttgtcc tttggatact ttatataaag
    13621 gcggtaacgc gatatatact ttcccagctt caatcagcgg tttcatataa cgataaaaga
    13681 aagtaagcag taatacttga atatgggcgc cgtctgtatc cgcatcggtc atgatgatta
    13741 ctttgtcgta attacagtct tcgatagaaa actcaggacc gactcctgca ccaatcgtat
    13801 agatcatcgt attgatttct tcatttttca agatatcttg cattttagct ttttcggtat
    13861 tgatgacttt tcctcgtaaa ggtagaattg cttggaactt tctgtctctt ccttgtttag
    13921 cagatccgcc agcagaatcc ccttcgacta aatacaattc atttttctta gggtttcggg
    13981 attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc ttcttcccat
    14041 ttcggctttc ttctctggct ttacgtgctg cttctctcgc ttctctggct ttgattgctt
    14101 tacggaccaa catctggctc atttcgctat tttcctgcaa ataaaagccc atttgttcac
    14161 tgatgacgtt atctactacg gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc
    14221 cttcaaattg cagaagattt tcaggtacac ggatagaaag tacagcagct aacccttctc
    14281 tgaaatcact accttccaga ttcttgtccc gttctttcag caaaccaact tttctcgcat
    14341 attcattata agatttcgtc atcgccgctt tcataccagc ttcatgggtt cccccatctt
    14401 ttgtccgaac gttattgaca aaagataaaa cattttctga ataaccgtca ttgtattgat
    14461 aagcgacctc tacttcgata ccttcttttt ctcctgaaaa ataaacaact ggtgtcaatg
    14521 tatctttttc ttcattcaga taatcaacaa attctttgat tccttcttcg taatgaaaaa
    14581 tttccttgac tggttcttct ccacgaagat cagaaagaga gattttaacg ccttttagaa
    14641 gaaaggcaga ttctcttaac cgttcggcta aaatttcata tgaaaatttc gttgtactga
    14701 atattgtatc atctggtaaa aaatggacag tcgtcccgtt tgcttttttt gtttttccta
    14761 tctttttcaa agtaccgtca ggtttaccgc cgtttttaaa tttctgctga tactctacgc
    14821 catctctaac gatcgttaca attagccatt tcgataaggc atttaccaca ctggctccta
    14881 caccgtgcag tccaccagaa gttttgtacc ctccttgacc aaactttccg ccagcatgca
    14941 gcacagtaaa aatcacttca actgttggta ttccggaagc atgcattcct acaggcatcc
    15001 cacgtccgct atctgcaaca gttatactat tgtcttcatg gatggtcaca tcaatctcac
    15061 tgccataacc tgataatgct tcgtcaactg cattatcgac gatttcatag actaaatgat
    15121 gcaagcctcg gctgtcagtg gatccaatgt acatccctgg tctttttcta actgcctcta
    15181 atccttccaa tacttggatg gacgagtcat tgtattcgtt gtttactttt tttgccaagg
    15241 caaacgctcc ttcaataata ctttgcggca aaaccgcacc acgattatca tactagaaaa
    15301 ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta attatctcct aaccgctttt
    15361 tcgacattcc cctctatcga tcatctcttc tttgtggtca tttttatttt tggttttctt
    15421 tcaacttacc aagttcaatc ttaatacaac gatccattac gatatctgta cgtcctgctt
    15481 ttcttagcat ctcagctgct tctttgtttt caatccctaa ttgtgcccag aacactttag
    15541 catcagtttt caaaaaatca cctgctattt caggtaaaaa ttcactccgt ctaaatacat
    15601 ctacgatatc gattggatca tcgatttctg tcaaattcgc atagactttc tcgcctagaa
    15661 tttcttcacc agccaataat ggatttactg gtatgatttt atatccattg tcttgcaata
    15721 cctgtgcaac catgtaactt gttcgttctg gtttattgct caagcctacg acggcaatat
    15781 tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt acagacattt
    15841 ctgccacccc ttgcttttct ttttattcca tctattatac ttgtatcttt cattttttca
    15901 attgttcctg ttacctattc atgctaaaat aaaggatagt tttcaacttg aaagagggta
    15961 aaaaatgaaa ttgatcttat tacttatttt agcatatctt ttaggttcta ttccttccgg
    16021 tgtctggatt ggcaaattgt ttttcaaaaa agatatccga caacacggga gcggtaatac
    16081 tggaacgacc aatacatttc gtgtattagg caaaacagcc gggattgttg tcctgcttat
    16141 ggatatttta aaaggtacat tggctacttg tttgccgatg atcttccacc tttctgggat
    16201 taaccctctt tggtttgggg tatgcgccat tcttggccat acatttccga tttttgctgg
    16261 gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt
    16321 attttttgtt tattcttgta tcatctttat cgttagttta ttttgtacaa gtatggtgag
    16381 tttgagcagt atgatcagtg cagtattgat cactttatct acgatcgtcc ttccttatgt
    16441 agctcctgtg attttggcta agcccaattg gttactgacg atcattgcct ttttagtttc
    16501 ttcctttatc ttttatcggc ataaagacaa tatcaagcgt atcagaaacc atactgaaag
    16561 tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc tgatactttt tcattccaaa
    16621 gagataaaac gggagtgtga caaatcgatg tcacactccc ttattactta caggatagct
    16681 tttttacaca ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa cctcttactt
    16741 cactgtgatg gaatattttg tcttgaagat ttcatgggca cctagttgat tgatccctac
    16801 tttttccatc aagtttccac tactgttcac cgtgtcagcg attccgcacc acggttcgat
    16861 acaaacgaaa ggcgcttctt ttggataagg agaccagatc cctacataag gcatatcttt
    16921 atagcttaaa gttacgctat gggaagattt ttcacttcga atggcgtacg catttagacc
    16981 ccttgtttca aaaaccaagg catcttgctc aaaaagttca cgattcaaag ccagattcgt
    17041 atttgtctga cctaacgtct tttgatccat atcgatgaat gggcctttca atggtaggcg
    17101 taatcttgat ttcaatggag aaaatgccag atagaaatct tcaaatgaca atccttcttc
    17161 aagaggtaca ttgaaagcag gatgtccacc gatagaaaag aacatttctt tttcaccggt
    17221 atttgccact tgataatgaa ccgtgatgcc gtctcctcct aattcatagc tgatcactaa
    17281 ttcaaaatca aatggatact tttctaatgt ctccggcgta ctccttaaga tgaaactagc
    17341 catttcttgt ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc
    17401 catttgatat gttttccctt gataaacata ctgatcatct tttaaacgtc caacaaaagg
    17461 gaataaaacc ggtgcatgtc tgccccaata cgctggatca ccttgccaga tatactctaa
    17521 accatgttct tttgatttca gactgatcaa ttccgcaccg tgttcagcga tttcggcgat
    17581 cagctggtca ttttgaataa taacagtcat tgccattcct ccaacttcga cttacttttt
    17641 aaaatgggaa ggctggaaca aacatttcta tctgccccag tccccttact tctaataact
    17701 gtgttttgct aactcgtctt taaaacggga attcaatact tttaaataag ttcccttcat
    17761 gcctaacgaa cgagattcaa tgattccagc tgattccaat tttcgtaaag cattgacgat
    17821 cacagaacga gtgatgccga tactatcggc aatattagaa gcagtcagac gaccttcatc
    17881 tccatccaat gcttcaaaga tagcttgtac agccttcaat tcgctataag ataatgtgtt
    17941 gattgccatt tggacagccg ttgcactccg tacatctgct tcgatgcttc gagacttctg
    18001 ataaaggatc tgcataccga caaccgtagc tccatattct gctaagacta gatcgtcatc
    18061 tgcaaacgat tgttccatac gagataagat aatcgtaccc aaacgttcgc cagctccaaa
    18121 gatcggaacg acagtcgtca gaccaaatgg atatttctct cttgactcta ccggaaatgc
    18181 agtaatatca ctagtaattg ggatattagc ttcagttttc atcaactgtg ccactgtatc
    18241 agtataagat tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt cattgttcac
    18301 atcgtgacgt tcattataac ctagtaatac tccgtcactt cctatgatat aggtgttgct
    18361 gtcaagaata ccacctagtg tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc
    18421 aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt
    18481 cattgtcgtc ctccgatttt ttataaaata tagcgactta aatcttcatt tgccgcaatt
    18541 gagccaagtt tgtcatccac atatgcttct gtgatcgtta tttctcccat ttgcatatct
    18601 ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg
    18661 ccgatattat ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg ttcaattgct
    18721 tcttttgtaa acgtaactga cacattttct gtaccgatca acgcgacgta ttgttttact
    18781 aaagcattat ttggttcggt cagaatcttc acaaaatctt cagctgtcaa atcatctaat
    18841 tctacccgta tcgggaagcg tccttgaagt tctggaatta gatcacttgg tttagacaaa
    18901 tggaaagctc ctgaagcaat gaataggacg tgatctgttt ggatgatccc gtattttgtg
    18961 tttacttgtg aaccttcgac aattggcaat atgtctcttt ggacgccttc tctggatact
    19021 tctccagatt gctgtgattt agaagtgatc ttatcgattt catcgataaa aataattcca
    19081 ctttcttccg ctaatttgat tgcctcacta tggatatcag catccttgac taattttgct
    19141 gattcttcct tgatcaataa ctctaaagct tctttgactg ttaccgtccg ttccactttt
    19201 ttcttcggtg tcaaagcacc tagagtttca tttaggtcga tccccatttg ctccaatcca
    19261 ttgttcatca ttggtgcagg tttcttttgg tcttcaactt caatggttac ttcacgacta
    19321 tccagtaaac ccttttgtag ttgttccaag actgtacggc gattgattcg aatatcttct
    19381 gtcacttctt cttgcggttc agcggtattt tgctgactag cattaaaaag attcatcatt
    19441 tgttcaaatt gattagtgga ttgtttctgt tcttttttga taccaggtac caagattttt
    19501 accaaacgtt tttctgcatt ttttttagca cttgtgtata cacggctgta ctgctctttt
    19561 tctacgattt ggatcgcatt ttctactaaa tcccgaacca ttgattctac atctcttccg
    19621 acataaccga cttcagtaaa ttttgttgct tcgactttga caaatggtgc attgacgatt
    19681 tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg taggtccgat catcagcata
    19741 ttttttggtg taatatcttg ttgcattttt tcatctaact gcaaacggcg atagcggtta
    19801 cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc ctacgatata ttcatctaat
    19861 tcacttacga tttgtcttgg tgtttgtttt aattcataca tatctactca cctcttacag
    19921 ttcttctacg ataatattat gattagtaaa gatacagata tctgcggcaa tatttaacgc
    19981 attttctgca atttctttcg cactcatgtc gttttgagca tagtttttca ttgctcgggc
    20041 tgcagccaac gcaaaattcc ctcctgatcc gatagctaag ataccatcat ctggtgtgat
    20101 aacttctcct gtacctgaaa caagaagcat ttctttatca ttcattacga tcagcatcgc
    20161 ttcaagtttt tgcatagatt gctgtgtccg ccattcttga gctaattcga cagctgcgcg
    20221 ttgtagattc ccgttatatt cgtttagttt gccttcaaat ttttcttcta aagtaaacgc
    20281 atctgcaaca cttccggcga aacctacaac aacttctcca ttgtagatac gtcgtacttt
    20341 ctttgcagtg cctttcatga caacagattc acccattgtt acttggccat ctccagccat
    20401 agcaaatttc ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg gtgattcgac
    20461 catgtttgtt tcctcctaaa tttgttcttt tccttatttt tacgctcttg gatggaaaga
    20521 acgatagttt ttttgtaaac tttcttttgt tacatgggca tagatctggg tagtcgataa
    20581 attggcatgt cctagcaatt cttggactgt ccgcaaatct gccccattat ttaataaatg
    20641 agtagcaaat gtatggcgca gcatgtgagg atggatatca ctatccaaac tgcttttttt
    20701 gatcatttga ttcaagacat attcaattcc agtaggcgtg attggttttc catgatgatt
    20761 cacaaaaacg atatcatgct cttcgtgata ttttgtcatc aatacctggc ggccttccct
    20821 gaaatattct tgcagagcat cttgagcaaa agaacctaga ggagcatatc tctctttgtt
    20881 ccctttacca tggatcaaca tgacctctga atcaaaatca atgtctgata ccgacaaact
    20941 gctacactca cttaaacgga tgcctgaccc gtaaaggact tccagtaaag cgcggttcct
    21001 caaatccaaa ggactatctc ctgctacgct gtcaaaaagt gcttgcattt cattttcata
    21061 aaaaaaacgt ggcagtctca aatttttctt tttgagatga acgtaagaaa atggattttc
    21121 ttttatcact tcttctttga gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg
    21181 actgatagaa tttctgctat agctaggacg atgatcagct aatgcactca aatagacacg
    21241 aaccgtaaaa tattccgcct tcagcagatc agcgtctcca gattcctcca aaaaatgttt
    21301 gaaatggaga atgtcttctt catatgcttc tttcgttttt tctgaatatc ctctttcgac
    21361 aatcaaataa cgcaaaaatt tttgttccca cgtggcttct gtcattttct caccttcctt
    21421 ttgtcccaga gaataattta gcacagcgtt tataaaaaga caaataaatt gtcagaattt
    21481 aaccaatatt tctaagtttg ttcacaattg gcgcaattcc tcacattttg tttgagaaat
    21541 tcttaagaaa aacgcaagaa aaagagcgag actatccgtc tcaccctctt catggagttt
    21601 ccttactcat ttgggattgc agttttttct aattctgttt ttattgcctc attagctttt
    21661 aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt
    21721 tcaggaaaca gaccatagtt ggcattcatc ggttggaaat gttttccttc cgcatgagta
    21781 atgtagtaag ccatacttcc aatagctgtt tcttgtggaa actcgactgg ttccatttct
    21841 tttgctaagc gtgcggcgtt gattccagca agtagtccgc tcgctgcact ttcaacatag
    21901 ccttctacac cagtcatctg acctgcaaag aacagatctt cgcgtttcgt tgattggtaa
    21961 gtcggtttca gcaattctgg cgaattcata aagctgtttc ggtgcatcac accatagcga
    22021 acgaattcag cattttctaa cccaggaatc atccggaaaa cacgtttttg ctcgccccat
    22081 ttcaagtgag tctgaaagcc cacgatgttg tataaagaag ctgcagcatt gtcttgacgc
    22141 agctggataa cagcataagg acgtttgcca gttttaggat cttccaatcc aactggtttc
    22201 ataggtccga aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat tggcatacat
    22261 ccttcaaaat atttttcttt ctcaaaagat ttaagttctg ccacttcggc attgattagt
    22321 gcctcatgga aagctttgaa ttcctcttct gtcatcggac agttcaagta agctgcttct
    22381 cctttgtcat agcgggactt caaatatact ttatccatat cgatcgtagc tttgtctacg
    22441 attggtgcag ctgcatcata gaaataaaaa ccatctgaac cattgaatgc tttgatgctt
    22501 tctgctaatg gatgagaagt caaaggtcct gttgctacta cggtgattcc ttccggtatt
    22561 tcagtaattt cttcgtttat cactgttact aatggatgct ctttgacttt tcgggtaatt
    22621 gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac cagctggcac tgctgtttca
    22681 tctgccgaag tgattactac cgaattcaat cggcgcattt cttctttcaa taaccctact
    22741 gcattagcca aactgtttcc tcttaatgaa ttggaacata ctagttcagc aaaattactt
    22801 gtgtgatgcg cctcagtcga tttgactgga cgcatttcat ataaacggac agggactcct
    22861 gcttgtgcga tctgccaagc agcttcactt ccggcaagcc ctgctccaac aacatttacc
    22921 attttcaatg aaagaacctc ttttctatta tttttggatg tcttcttcgt aatctccatt
    22981 tacacagact acttgtttac cgcctttgac tttcttctcg accagatatc cgtcacattt
    23041 tggacaattt ctgcccactg gtttatccca agaagtgaat tcacattctg ggaaacggga
    23101 acaaccatag aatagtctgt ttttcttaga ttttcgttca atgacttgtc cttcatgaca
    23161 tactgggcaa gtcacaccga tttcttttac gattggtttc gtattccgac aatcaggaaa
    23221 attactgcaa gcataaaact tcccatattt ccctaatttt atcaccatcg gatgtccaca
    23281 gacatcacaa tcgaatcctg ctggttcatc tttgatttga attttttcga ttttttcttc
    23341 cgcattcgtc agttcttttt caaacggttt atagaaacga tcaaccactt tgacccacgc
    23401 ttccgtgcct tcttcaattt tatccaaatc tccttccatt tctgctgtga aatggatatc
    23461 gacgatttga gggaagaatt cacagattaa tgaattgaca atttctccta gttcagtcgg
    23521 ctcaaatctt ttttgtgtca attttacata gtagcgtctt tggatcgtat caagtgttgg
    23581 tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca acgtagcctc
    23641 actaaaacgt gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa cagattggac
    23701 tacatcgcct tcttcaagtt ctggaagaat attttctttc tcttctttgc cgtcatctct
    23761 accttccaca tacacttgca tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa
    23821 gatgacacca ttttgttcta atgtcacttt cattgtatct aaaatagctg gtgtcatctg
    23881 actagccaca aaacgtgacc agatcaatgt ataaagtttc aattgatctt tatctaaata
    23941 ttgtttgagt tcatctggtg tacgaagcac actcgttgga cgcacagctt catgggcatc
    24001 ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt cttttccata
    24061 cgtatcttca ataaatgctg cggcctctgc ttttgctgta tcggcaatcc gggtagaatc
    24121 agtacgcatg tatgtaatca agccgactgt tccttgttta cccaaagtga ttccttcata
    24181 taattgctgt gctaccatca tggtttttct tgttcgaaag tttaacttac gggcagcttc
    24241 ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt cttttgcgct ctttcttttc
    24301 tactttttta acttcgaagt ctgttccatc aattctagca gtgacttctt ttacactttg
    24361 tgcatctggt aatttctttt tcttaccgtc gattccccag aaatttgctt tgaatttctt
    24421 gcgagctttt ttaaaattac cgtcgatgct ccaatattct tctggaacaa attctcggat
    24481 ctctttttca cggtcaataa taatttttaa agcgacagat tggacacgtc ctgcacttaa
    24541 ccctttcttg accttacgcc agagaatagg actgatcgaa taaccaacga ttctgtctaa
    24601 ggtacgacga gcttgctgtg catctactaa atctacatcg atcgttcttg gttccttaaa
    24661 agctgctttg actgcgtctt ttgtgatttc attgaagacc acccgatttt tttctttcag
    24721 atccaaccca agtaggtacg ctaaatgcca agcaatcgct tctccttctc tatccgggtc
    24781 acttgccaag taaacttttt gagctttttt tgctgcggct ttcaggcttt tgatgacatc
    24841 gccttttccg cgtatagaaa tataatgggg ttcataattg ttttcaaaat cgatacccat
    24901 tttacttttc ggtaaatctc ttatatggcc aacactggcc attactttat agtttcgtcc
    24961 aagatatttc tcaattgtct tggcttttgc aggtgattct acaatcacta aatatttata
    25021 cgccattact gaatggactc ctttccattg tcgtgttcct actattaaaa gaggattcat
    25081 acaaatcgta gctaatcata tccattcaga aaacgtttgt caagaaaagc aaatgaaaag
    25141 tatggtgtta ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg caaactgcac
    25201 cgtcttggat caaatgatga catccggtag aatgttctgt taaaatattt cctggaacag
    25261 aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt tatcagagag ccgctttttt
    25321 tcttggcttc aaagacacat gttcctttag acaaagctgc gataatccga tttctcattg
    25381 gaaaacaatg ctttttcggg ccggattgag gaggatattc actcaaaaga agatgatcat
    25441 ttgcaatcac ctcttgcaag tcacgatttt cagcaggata gacttgctct atccctgttc
    25501 caatgactgc gattgtccgt ccaccatgag agattgctgc ttggtgaccg tatgtatcta
    25561 tccctttcgc gcagccactg acgatcacat atccagcttc tatcactggt tttagtaaat
    25621 aataaactgt ttttttggct aaagcagaag tgtgtcttcc accaacaacg cttaacatgt
    25681 tacaagaaag taaagataga tcaccattat aaaacagtac aattggcgga taggtcaagt
    25741 gaagcagctg agggggatat ttgggatcaa agcaagtgat aaacgactgt ttcgattgga
    25801 ttttgcccca gttagcgtga atttcctgcc aacttttgcg aaacagctcc cggtgtttgg
    25861 ttattttacc tatcttaatg atgtcttcaa atccgatttc tttataagga tactgcatca
    25921 tccagttagc aagtgcccat tttccttgac tactgattcc ttttgtataa gcaagtttca
    25981 ataaattttc ttctatttga tacatcataa aaccgccttt cgtctctctt tctaataaga
    26041 tacgtaaaag cggctgtttt ttattgtaaa tattttttta caggtgcaaa acttttacga
    26101 tggattggcg taattccttg tcgttcaagt ccttctaagt gttcctttgt tccataacct
    26161 gcattatgcg agaaaccata tccaggataa agcacgtcat attctttcat caattggtct
    26221 cgatatactt tagcaatgat acttgcagca gcaatcgaca cagatcgtgc atcccctttg
    26281 attatttttt cctgctgaat aggcaaatcc aaggtcatcg catcgatcaa caaatagctt
    26341 ggtacaatgg acaatttttc tactgcttgg atcatcgcta atttacttgc ttcataaata
    26401 ttcacatcat cgattgtctg ctgatcaacg atcccgatcc cgattgccag ggctttttct
    26461 tgaacttgtt gataaagaag ttctcgcttt ttctctgata attgtttgga gtcattcagc
    26521 cctaatatga tctcattttt cggcaagata actgcagctg ccacgacagg tccagctaat
    26581 ggtcctcttc ctacttcatc aatgccggca atccattggt gaccttgtgc gtacccttgc
    26641 ttttcaaagc aaaacatttc ttcatatttt tccttaatta atgcttcctt tttcaatcgt
    26701 cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg gatcatttct taatgattct
    26761 agtgtaggat catctattct ctgtatgctt ttcaatcgtt ctttgatact ttggatggat
    26821 tcagtcatca cttttttgct ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt
    26881 tccttgacga atatcatgca cgactaattc gctgccacga tcatagtctt ctttataccc
    26941 acgttttgct gtaatatcca tcagtaattc cggtgccaat ttttcttctt gctcgattga
    27001 taaaccataa cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta
    27061 aatcgctaaa tcatctaaat ggacaagctg gtctttgatc gcgcctgtca gcgctagttt
    27121 tttgccgatt tcttcatctt caaatttggg ccataaaatc cctggtgtgt ctaacaattc
    27181 caactcatta cctaaacgaa gccactgctg accttttgtt acgccaggct tatttcctgt
    27241 ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta gatttcccaa catttggaat
    27301 acctaagacc attgcccgaa tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg
    27361 tttttctttc aatgcttctt tagccgcagg tactagacct ttaattcctt tactttcttg
    27421 tgccgtgata gctaatgcaa gatggccttg tttacgaaaa tattcttgcc acagctttgt
    27481 ctgttgtgga tctgccagat ccgctttatt caataaaaca aggcgaggtt tttgctgaat
    27541 gatctgatct agcattggat tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa
    27601 tacgatgtcc acatatttca ttttttcaga aacttctcgt cgggcttttg ccatatgtcc
    27661 cggaaaccat tggatggtca ttgttctctc cattcctttc ataccggaat attccccggc
    27721 tttacttgtt tttttctttt tcaatataaa ttcacttttt tattataaag aaattccatc
    27781 tattagttaa gtgaaagtga accagtcctc attctatcac atcttcactt ttttgatgat
    27841 gcaaattttt attttggttt ctatatatga atctaaattt ttgacagcgt ttcctaaata
    27901 agagatgata gacgaacaaa acagaaagga gacgaaacaa atgaatagta tggtttttgt
    27961 taattttcct gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc tgggttttaa
    28021 aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg gtatgggatg acactttttg
    28081 gataatgctt ctctctcatg atttttataa aaaatttata aaagataaag aaatagctga
    28141 taataaaaaa acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa
    28201 aaaatttgct caaattgcaa aagaaaatgg tggtactttc cacgaagtag atatgggaat
    28261 gcctgaagaa gaaatgtttt cattagaagt ccaagatcta gacggaaata cattagaacc
    28321 cgtatggatg aagatgtaag cagataacta atctgacgtt ttttataaca aaaaaacaaa
    28381 ggcggatgat acgataagta ttctccgcct ctattttttt tattcgtaca atctcttttt
    28441 tacagtttat aaaagcagtc ttctcaccct ttttttaatt gttagatgcc agtgtatgta
    28501 aatcaccaat gtgtcatgac acattggtga ttttatggta tccttttatt attaactgac
    28561 aaaggagaga atattttgaa cactttgatt gcaaaagacg tttttagatt gcccgaaatg
    28621 aatgcaagat tgttgtcgac ctctgctctt ttattagccc ttgatgtcat cttatacaag
    28681 atagcgattg gtcctgctta ttttcaaatc acattcggat ttttaagtat ggcgcttatg
    28741 ggttatctat atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa cactttaaat
    28801 ttcacgatat ttggttcagg aacgtttcaa gctgcttttc ttatcacggc tttccttggt
    28861 ggatttatta atggcttatt tttatatcat aaaaagataa acctgccttc cgtcatcgct
    28921 gcccaattat tgattatgat cccagtcagc ctttttatca attcttggct gatgtctgtt
    28981 ttgtttggtg cagatttcaa agtaattttt ctaggtagat tcatcaggaa tatgatccaa
    29041 atccctattc aaataatggt tgtttattca tttatcaaaa ctttagaggc tagaaaagtg
    29101 tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg attaaacaat
    29161 aaagcgtgca gtctcaacag actgcacgct ttattgtttc tcactggatc cattttattt
    29221 catcaagtgg ataatagaca aatcttgctt tacctagaat atcagtttta ttgatcgtgc
    29281 caaacgaacg actatctttt gaaactcttc gattatctcc aagtacaaaa taattatctt
    29341 ccggaagctt ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta taggatgcat
    29401 ttgcatgatc tgatttcagg tttttagtta gataaggttc cttaactact ttcccattga
    29461 tcttcaattg atcattttgg tagctgacat tttctcctgg aagaccgatt actcgtttga
    29521 tataaatcgt gccatctgct aattgaaaaa caacaatatc aaatcgtctg atctccgaaa
    29581 atttttccat cacaaccata tcaccttgct tcaacacgtt ttccattgaa ttgccttcca
    29641 ctggtaccgg gatgaataaa aatccccgca acatgaacgc aaggaataca gaaacaagta
    29701 agtatttgaa tacaagccag aagcgattaa tatagcgttg tttttttgtc aatctctatc
    29761 aatccatccc tctttttttc attctttctt tattataaga aaaatatcaa aaaaatgcga
    29821 ctagatagct gaaataaaaa taattctttc aattaggcta ttggacaaaa aacttttttg
    29881 atcataaagt acaaaactat ctcttatatg tcatgattat tttctaaata tttttcccat
    29941 gcttcgtcaa agatatccat ggacggcaaa tagtcgacat tcagttccag ataattagaa
    30001 atttcttcgt agctagagct gtgcttagga aattggatgt ctttaccggc ttctgtagcg
    30061 aaaacagata aagcatcatg tgtgccaccg cctcgcagtg tcattaaata atgaaaaaag
    30121 cttcgattca cccttcgttc ctccaatttt cttcgataaa ggcagcccgg cgtgcatggt
    30181 ttcttttaaa attttcagga tgctttttat aatactcttg atggtcttct tctgctagat
    30241 aaaatggttg ggctggttcg atttttgtaa cgattggctg ttcgaaacgt ccactttcag
    30301 ctaactgttt cttacttttt tcagcaatga tccgctgttc ttctgtcaaa taaaagatca
    30361 ccgggcgata attgtcaccg cgatcctcga attgtccgaa agcgtcagtt ggatcagttt
    30421 gctgccagta aagttctact aattgttcat atgaaatcaa tgttggatca tagataatct
    30481 ccactgcttc tgtatgtcca gtcgtttggc tttttacttg ttcataagtc ggatctggta
    30541 catgccctcc agtatatcct gacatcactg tatggatacc aggaagcgtg tcgaaaggct
    30601 ggatcataca ccagaagcag cctcctgcaa atattgctcg ttccattatg attccccctc
    30661 taatactact catccgatga ttttttattt tcttttgtct tgctttcagt actgctggtg
    30721 ctagttttgt tggtatttga gttgtttgaa gcatcagagg tatcttttgg caaatataga
    30781 ctaaaccgaa tgtcatcatt tactaagtcg attttatctg ctttaatata catcccgttt
    30841 tgcattcgga attgatccaa cctaagcatg atagtcagat catctgtatt gacttcgatc
    30901 caatctggta atttatagtt tctcttgatc atattcatca catctttgat tggcaaactc
    30961 aatgttccga tagaaagact tctagctttt agttggacat ttccgttttc catcacatac
    31021 ggatcaaaat ataaatagaa atcgatagga aaacctagta cttcgaaagt accattgagt
    31081 agtgcttcat tttccaaata aaatttatat tcaatatcac tatctttttg atactcacta
    31141 aggaaaaagc tgatgatttg attgactttt tctttatttg aattgatcgt taagacagga
    31201 tctccttgtc tttccgtaag agctggtatt tctgggagtt ctggttcccg attagcaaaa
    31261 atacggctgc ctaaaaatac ggtcgaacca agtactaccc caagaaggat aaggaaggcg
    31321 aatttccagt aatttgtttt ctttgttttg tttgaagata cgtcagatct tgttttcatg
    31381 tttattgatc atcctccttc tctaaccatt ggttttgtgt ctcgatcagc tgatctctca
    31441 ctgcatttgc catcaattga taacctaaat tatttggatg gaaatgatct tcttcataga
    31501 gcacattatt tttgatgcca tttagatttt ctgtcgtgct agttgtttga tctgttcctt
    31561 cttctgtgac acctacttcc tgattcaatc cttgataaag caaatcattg attgggataa
    31621 agtagcagtt cggcattttt tttgtcaccg tttctgttcc tttgttccaa ttgttaacaa
    31681 ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag atgcctaaaa
    31741 tgtaaatagg tgcggtttta ttgagggatc gaaattcttc caatagttct gtgacatttt
    31801 cctgatagtt tttcagtggc tttttaaagg aattgaccgt taacccgaat aaattatttt
    31861 ggataacttt catcaaatca ttaccgccaa cagtcagtgt gataaaatca gctgttttga
    31921 tattgtttcg aatttcttct tttgttttta ctcgctttaa gatctggtca cttcgttcac
    31981 catttacacc atagttttct gtttcaaccg cggtcaaatt ataacggtct tttagatcat
    32041 ttgcgacaag gggaacgaat cctccttgct tggtttgatc tcctactcct tcagtcagcg
    32101 aatctccgat agctatgtag tgaagttttt cttttttatt tggatcttct ctcacagtag
    32161 ttactggttt cattagtggg ccagctttcg gtatagaagc agctaaacct gcatagatga
    32221 tgatcgcaat taataatggg aacaaaacga ggatcaaatg tcgtttcgtt aatttcattt
    32281 tttcacctct tgtattcgat tggttctaat gttatcaaaa attttatcaa aaaggtagtc
    32341 tttatgaaaa aagaaaacaa ttattttcct aattttataa agaaaaagag aataagctgc
    32401 aagcctttca ggctgagggc ttatcctctt ttcgactaat ctacataata catgattgca
    32461 aacgcgtctt taccagtatg cgtagcgata accggattcg tatgcagtac agggatatgc
    32521 atgtccggga ataattcttg caaaccctct ttgaattctt gcgccaattc ttttgcatcc
    32581 gcatgtgaaa taccgatttg tcgcacattt ggcaattctt tcagttcatt tttcaaatta
    32641 ttaaaccatt tatcgaacgt tttctttcct cgccccttag ttacagggat taattcagaa
    32701 tgggcgaaat tcatcaccac gcgtatgttc aaaacactag acagcaaacc agttgttcgg
    32761 ctgattcttc caccttttac caagttatct agtgtggata ttccgatgta taattttgta
    32821 ttgtcttgta cttttttgac ttcttcaagg attttctcta ctgttttacc ttcttttgct
    32881 aattcagctg ctcgaattac ctgaaaagat aatccttggt cagtggtatc actgtcgata
    32941 accgttactt tcgtttttgt tagattactg gcttgtcttg ctgcttctac tgttccactc
    33001 aagccttttg tcatatggat cgaaatcact tcactgccgt cttctcctag ctcatcatac
    33061 atcgagatga attctccaat tggtggttgg cttgttttgg gcagattttt tgctttcgcc
    33121 atcatttcca taaatctttc gccgtctagt tggtcatcat cgggatatac tgttccgtcg
    33181 atcatgatgg atagcggcat gacacgaatg cctaaagcat cgcgtacatt tttttccatc
    33241 gtacatgaag aatctgttac gatttttaca tttgtcatta ttttctctct ctctttcttt
    33301 aacaagatgc cttattcatg ttaaactaca tgtaagacaa tttctatttt tagtataaca
    33361 gacaaataat gatttttcat taattacacc tagtaaagga gccgttttca tggaaaaaac
    33421 gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca cccatggaat
    33481 tgggactgga ttgagtatcg cgggactagt ggttctttta gtaaaaggtg cccacttgca
    33541 ttcctccctc catattgtct cttatgctat ctatggatcg atgatgattc ttttattttt
    33601 aacgtctact ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt tccaagtatt
    33661 cgatcatagt tcgatttttc ttttgattgc aggaagttat actccttttt gtttgctgag
    33721 tatccgagga tggctaggtt ggactttatt tgtattaatc tggttgtttg cgattcttgg
    33781 cattatatat aaagcgttga cgttgcataa acgggagaga gtatcaaaag tatctacgat
    33841 catctatatt tttatggggt ggctctgtgt cgtggctgct gttcctcttt atcactctct
    33901 tggaatgact ggtgttattt tgatggtagc aggcgggctt tcttacacga taggcgcatt
    33961 cttttatagc ttgaattcta tccgctatat gcatgttgtt tggcatttgt ttgttatgtt
    34021 aggtgcaggt tttatgtact tctctgtttt attctttact taataaaaaa aataaagcta
    34081 tggatgggac agctctaaat aacagctagt ctatccatag cttttttata tcgtcaattc
    34141 gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt gatgcggata
    34201 acgatttttt tcatccattt ctccttgact agtattgatc agtgaccaat cagaccagtc
    34261 aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac ggtataatat
    34321 cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga aaaagatacc
    34381 gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat gcacgccttc
    34441 tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca acggacggct
    34501 acccatccct tcgaatgttt ttcgtcccat cactagtgta tttgcttctg tcatttgttt
    34561 gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc caatcaatcc
    34621 gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga ttctaaacag
    34681 cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata tcttccatct
    34741 caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat gcgcgtattt
    34801 ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga gcatctccta
    34861 atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg tgagtcagca
    34921 acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg cgttgataca
    34981 attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga caaggaggca
    35041 aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt ctagaatcag
    35101 gagtcttttt gattgcttcg atcacttgtt tgatttgatc gataaagcca ccgtcttttg
    35161 tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat gtttcagcaa
    35221 aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta tattcttcat
    35281 tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt ccattatagt
    35341 ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt ttattttgta
    35401 atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt ttgatcaatc
    35461 caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca aaccgcattt
    35521 ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga tgcccttctt
    35581 ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct cctaatcttc
    35641 tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg cttctgtttc
    35701 agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat gattcatctc
    35761 ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa tcgtttccca
    35821 ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc tctgtatttt
    35881 tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat aactggacat
    35941 gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact tatctgctac
    36001 cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa aacttctaaa
    36061 ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt catccaataa
    36121 taaaacatta ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt tttctccgcc
    36181 tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc gttcaagtaa
    36241 ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag cttcttgtaa
    36301 gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat aatacgcaag
    36361 atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc cagctagaat
    36421 attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc tttcccgaga
    36481 ttgaatcaat aaatctaaat gttctagcag tgtttggtca tttatacggt aactgccatc
    36541 tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct ctaaatcatc
    36601 ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga tccgtgcttg
    36661 ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct gtttataaag
    36721 ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt tttctaagac
    36781 gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt cgaagattcg
    36841 gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg cgccacggta
    36901 ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt ttgttggttc
    36961 gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa ctcgtttttt
    37021 ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta atttttgcaa
    37081 aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt gttcttctgc
    37141 tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt cgatcaatgc
    37201 ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga ctgcttggag
    37261 aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac ggtaatcagt
    37321 tggataaaac actgtttgcc gatcaccgtc gctgctatcg ataccggcaa taatatttaa
    37381 gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt tttcgtggac
    37441 caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga ggtcagttac
    37501 ttttaattct ttcaacatgt tccctcctta tcttggatat tgtatcataa aaatagtgaa
    37561 cttcaggaaa atttttttgg tatttgttct ttttgtgttc tcaaaaaaag aacaggaccg
    37621 attcgatcct gttctgatat gttttgtttt ttataaaact tgaaaatttt taccttttta
    37681 ttttattgga ctcgtttttt ttgactttgc tcgaaacgca aatcggaaag aaaagttatc
    37741 agatggtttg ttcatcgtat aagattctaa caaattgata ttcctgcgat agctgttcgc
    37801 agaaaacgtc gtgatttttt cagcaacttc gtattcatca atgatttttc tttcaagata
    37861 ataacctctt agcatttttt tcgtaaacag actatcttgc tggatcatcc acgaagaaat
    37921 caaattcccc gagttcattt ttattccttc aagtttgcgt tgctggataa aaaattgaac
    37981 catcgtttca tcataaatat cagtcaagtt atctaaactt tcaataatca actgagtatt
    38041 tttttgatat gtctctctga tttcttggat atcttcatca ctaataaatg tctgacgatc
    38101 agcatatctt ctttgccaaa acattttcgg atggagaacg attcgcatta acctacggac
    38161 aacaatgaac caaaagccga taaaagacaa aatctggctt gtgatagaat gttcgaatcg
    38221 agaaagaaaa cgggagtaaa aacgataacc attagaagaa agcttgcccg aacgataact
    38281 ttcatccaat ccatcccgtt cgatcgacaa aatcagtgct tgtatttctt gtacttcttg
    38341 cttttcatca tcggttaaat cttccaagta cagatcacgt atccgttctt gataattttc
    38401 tatcactgct tcagtggcta aaaattcttt tgtattacga tcaatttcct ttatttcttt
    38461 tcgcaaaact tcaacgacct cttctaaaat catcaaagca ttcaaatccg tgcttttcgc
    38521 ttcctctgtt tccgtcagca taggcaagac aatgatccct atgaccaaag tcactaagat
    38581 gacacccgcc gtcagaaaca aaagcaaaga ccgctggtaa aacatcatat tattgattga
    38641 aaatggcaaa ataaaaatcg tagccaaact gactgttccc tttacaccgc caaatgttaa
    38701 gatcaagatc tcgttcaact gttttttgaa ctttttactg ccatctttga atacataaaa
    38761 aagactgata gaaatgaatc tgatcacaaa aagcatgaca gagattaata cgataatcgc
    38821 taataaaaga ccgtttggat ataaaccgtc tccccatact ggcgagaaca cttgcgtcaa
    38881 ttcgattcct aaaaatataa atactaatgc attcagtgta aaagcaatag ttgtccatgt
    38941 actgtgggac aagctcgaca attctgcatc aaacacagag attttcctaa aaccagaagc
    39001 ttggagaata cctgctgcca ctgcagcaat gattccagat acatcaaatg cctctgccaa
    39061 tacataggct agaaaaggaa gcaaaagttc gatcagcaga taccctgtga catcttgtgc
    39121 tgatgctttt tcgatcaaat gaattatttt ttgtttgaac cagacaagca aaaagccgat
    39181 caaagctccc ccaatactag aaactactag actcatcccc gcgtttactg ctgaaaaaga
    39241 tccggtgatc agcgcaccaa gagcaaactg gaaggcagta acaccagatg catcattcaa
    39301 aagaccttct ccttccaaaa tatgcatcgc ctttttaggg atattcactc gtccagaaag
    39361 agaactgact gctaccgcat ctgtcggacc taacgctgct ccaaaagcaa agcaggcagc
    39421 aagtgggaca cttggcaaca ggaatgataa cgtcgcacct accccgacaa gggtcaaaat
    39481 gactcctcca aatgccaaga acaaaattgt cccgaaattt ttgagaatcg aagaaatatc
    39541 tgctttttct ccttctctaa ataacaatgg tgcaataatc ataactaaga acaattcagg
    39601 ttcaaaatcg attgattgtc cccactctgt cagacctaat accactccaa ggaaaatttg
    39661 gatcagtgga gctggaatca ttggcaaaat gcggctagct atgttcgata aggtgatcgt
    39721 tactgcaaat acaataacta actcagcaaa ttccatcgtc catccccttt attttttatt
    39781 ttgtgtaagc atttttaaaa attgttcttt tttctttaaa attttggttt cgactacttc
    39841 aacctgtgtt tggttcccct ctaattcaca ctgtacacgt tttgactcca aacgaagcag
    39901 atcatgtttc agacgttcca actgacgata catcaatata tttcttttac gcaacttaca
    39961 aagtatttga cggcggtcat ccatcgaaag ggcgccatag cgatcatgca acagtttttt
    40021 ttgttgttca aaactctctc tcattttcca gatctcctat acacaaactt ccatcattat
    40081 agcaaagttc tttttggatg aaaaatattt cattgtttca tcgtttattg ctagttagaa
    40141 atttccataa atttttcaac gcctcattct attccaaata gtatcagata tcaagtaaaa
    40201 cttaagaaag ataaaagttt ctgacaaaag gatgtttact ttcccattcc tgcctttctt
    40261 gttcttttat cacaacaaaa aaatggggct gcgacaaaaa tcttgtcaca gtcccttatt
    40321 ctgaataaac ggtgtggaag agacagccta tcagtagtaa taagacacat ctctttcaca
    40381 gcctcatttt tgcgtaaaaa tcgatttatt cgaaaaattc cccgtataca tcttctacac
    40441 gttgatctgg attgatcacg atatacaagt tacctgtttt ttcgctgatt tttgttgatt
    40501 gatattgatt gtctaatcct tctgttcctt tatctagata tgggaagtac acaagatcag
    40561 gtgtgccatt tcggtaaagg atttccattc gctcgatatc ttcgtatttc aatgcgcgat
    40621 taaacatgcc catttccagt ccgcctaggt tgatatcatg tgtcgcaact gcgtcccctt
    40681 catggaagat ttcgatttta aatccagcac acggatggat ttcaacaaat tcactgccat
    40741 tgattcgtcc aaaacttgtt gtgatttgtt tgatccaaag atcgccgata tgacggcggt
    40801 tgatcgtcca ggtttctcca tttctaaaat aaaattttag cgctgtcatt tctttactca
    40861 ttaaaaagac tcctttatga tgtaatttat tcttgtacta cctcagttta gcacaaattt
    40921 atgtaagtgg tcacatctta tgttttttct tctaatgacg tatctgccca ctttaagtag
    40981 ccaacaaagc tgagcagata cagtctagtg ttttttaaat caatttttct ttcgataaaa
    41041 aatccaaaac ctgtttttta tcatttttta ttctttgttc tacgacggct ttttcagcta
    41101 attgcaaagc ttcactaacc cacttaccag gtttttttcc agtttcttct agcaagattt
    41161 taccatctac tgctaattcg cttaatgact tgataggtaa acgatcatag gattctccta
    41221 cggcatccat ctgaggtgtt tcgtcaaaat agatcatcaa ttcttctact tgcagtgcat
    41281 gttttttgcc taagaagtac aattcgaatg gttccaaagc tccagtttct cgttgtcgta
    41341 gtccttggac aagtgcttga acgtctttaa tcatttggtt agaacacttc cacgatttca
    41401 aaaaggaacg aatctcattt tcttttagat ccagttggct gatcaataat gcccatgctt
    41461 ggctctcttc tttcaatggg acggatggta attcagcaaa acgtaaaagt gcttcgcctt
    41521 cttctttcaa accgggacag caaatgtagc actctgtttc aataaatgca tggattcccg
    41581 cggcccgttc atttcccaag agcatcttga tgaattccac atttatacgt tctacagata
    41641 ttttacctaa tagctcgtgg ttttctttga tggcgtagaa ggtttcttcc tctaatttga
    41701 accctagttg actgacaaaa cgcaacccgc gcatcattcg taaggcatct tcatggaacc
    41761 gctcatgagg attgcctaca gctcgcaaga tctgttcttc tagatcttgc agaccatcga
    41821 acaaatcgat gatttctcca tcttctttca aagcaaacgc attgatcgta aaatctcttc
    41881 ttttcaaatc ttctgccaac gaacgaacga attcgacatg atctggtcta cggtagtcct
    41941 gataagttga ttccgtccga aatgtggtga tctcatattg ttcatcattt tctaatacca
    42001 acacagttcc atgttcgatc ccaatatcta tcgtttttgg aaaaagctgt ttgatttctt
    42061 ctggaaatgc gcttgttgcg atatccacat catggattgg ctggttcagc aagatatcac
    42121 gaacacttcc acctacaaaa taagcttcat atcctgcatt ctctaatttt ttcagaactg
    42181 gaattgcctt ttggtattcc agaggtaatt gttttaattt catttttcca acctttcctg
    42241 cgtgtttctt gtcctgagat aaagactcag ttgcatggtg actgtttgac aagtccgttt
    42301 cagagtcttc aaggccagga ccacttcatc ttgtttactc gtccttacgc tcaaagcgat
    42361 tatggtctct ttgattgaat ttttccatgt ttttttcaaa aacttcagtt aagtcgatat
    42421 tcaatgaatt agccatgatc atcgtaacga ataaaacatc accaagttct tctgctaccg
    42481 agttaactgg ttcagaagcc tttttgcttt tttctccata agtgtggttg acttctcttg
    42541 ccaattcacc tacttcttca gttaatcgag ccatctgagc taatggagag aaatacccca
    42601 ccttgaattg ctggatatat tcatccactt cctgctgcat actgtataaa gaacgctttt
    42661 tatccatctt tttccctcca aaactatctt accaaaactt taacgcaaat cctagataag
    42721 agcaattgag aaaatcgaat ttccatgcta catttaggaa ggaatttcta aatggaaatt
    42781 ctgtgaaaat acataattga aagctggaat tcatatggtc aaacgaaaaa cagtaaaaga
    42841 agttcttttt atcattatag gaacaagtat ttatgcattt ggtcttgttt acttgaacat
    42901 cgctaatcat ttagcagaag ggggagtctc agggatcacc ttgatcttaa gagctttatt
    42961 tggtattgat ccagcttata cgactttatt aatcaacatc ccattgattt tactaggagg
    43021 aaagatcctc ggtaaacgtt ccttggccta tactgttcta ggtacacttt ctttgtcatt
    43081 ctttttatgg atctggcaaa gggttccact agaaatcaat ttacagcatg atctactgat
    43141 cgtttctcta cttgcgggtt tagttgcagg tgtaggaagc gggatcgtct accgcatggg
    43201 tggaacaaca ggcggcagtg atattatcgc tcggatttta gaacaaaatt acggcatcac
    43261 tatggggcgt tccctattgg catttgatat catcgtctta cttgcttcat taacctatat
    43321 cgacttgaaa cgtatgatgt atacgttgat tgtttcgtat gttttcagtc gtgtgattga
    43381 ttcgatctta gatggcggat attctgcaaa aggtctactt gtcgtctcca acaaaagcga
    43441 agagattgca ccactactga tgaccggtct gcaaagaggt gtgactttcc tttctggaga
    43501 aggcgcattc tctggtaatt caaaaaaaat catctatatg gtcgttagct ctagtgaatt
    43561 aactgaagct aagaggatcg tccacgagat tgacgaaaaa gctttccttt caatcatcaa
    43621 tgtgcatgaa gtggaagggc aaggctttac ttatttgaag ccacagacaa gattactgaa
    43681 aaaactataa gaaaaaagac aaagataggc tatgaggaaa gattccacat ccagctgacc
    43741 tttgtctttt ttagtactat actattttta actaattgtt atgttcactt acccggttac
    43801 ttttcttttg tgtctgcaaa aggatctttc ggttaacttc atcagataat ttcaaaaaag
    43861 catccctatc tccatcttct agtgcttgat cgatttgagc ataaagcatt tttagttttg
    43921 cctcttcttc tttttcttga aaatatcgtt caattaattc agtatcttct tcactgactg
    43981 aagaatccca agaagaaaat ggattgtctt ctaagataga tagatattct cttgtctgcc
    44041 aagcattttc gaatatacat tccacatata aaggcttttt ccaattcaaa cggatttcat
    44101 gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa aaacaatttc atgggttccc
    44161 cttcaacaga tagatcactg atctgcagtc ctcgatccgc aaaattcgcc ccttcaacaa
    44221 agtggacatt gcttaaaatc gcttcatgat tggcaagata attcaagatc caaaaaactt
    44281 ctcttcggct aaaagataca tgattgacca tccacgttaa aaaattcttc ttttcccgta
    44341 catcaattag catttttctc acctctctgt taggtcatct aataatgaca gtgcctccat
    44401 atctcctggt tcaagtgtta aataataagt caataattct tgcgctcttt tcagctgccc
    44461 ttcttctctt aagaaaaagg catactcttt caagaaatct ggttcatgtg atagttcatg
    44521 ataagcctgt tcatagtgta cagctgcaac ggtaaagtct tctaattcat tataagcatg
    44581 ggctaggttc cattctgcat aaggattagc agtttcttcc atctgattga tcacttgtat
    44641 cacatcttca tatctttcat catccaagta aagattactt aaagtaagta atgtttcatc
    44701 ttgtttgtct cctacttcta aagctttcag taaaagttct tctgctcgct gtctatcatg
    44761 cagacggaaa gcattttccg aggccaggtg ataaagttcg acttggtaag gattttcttt
    44821 gattccttct tctaacactg tttgtgcttc ttcaatcatt tcttcttctt gaagagcttg
    44881 tcctagatat agatataacg attgataatg cggattaact tctcgaagtt cttccagata
    44941 gttgattgcc tgctgatttt ctttcaattg aagattcgta aaagctagct ggaacagtcg
    45001 atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt acgatagctg cttcgaattc
    45061 tccttgcata cttaaggatt gtcctaaacg ttcctgcatc gagattcctg aaatttcatt
    45121 tatcccgctt gccaacaatg tttcataaac cactgctgcc tctttgaaac gatcaactga
    45181 aaaatacaat tcagctagcg caaactggat caatggttca tcaggaagca acccagctgc
    45241 ttctttcaat tttgcttcac tcacttcagg gattccgata acttggtaca aatcagctat
    45301 cgccaataag ctttgcggat agtaatcact ttgcttatcc actttttcca ggtattcaaa
    45361 agctaagtcg atctcattat cttcgattgc aatttctgct aacgggatgt tcagtccgtc
    45421 attttctggg tattggttga tcagttgttc aaaaatctgt tttgcttcga tcaaaaagcc
    45481 aatagccagc aattcttcgc ctagttcagc taatatatct gctgggtctt cttttaatgc
    45541 tttttctaaa aagatttgtg cttgtgacaa gtcttcatct gataaagctt gcaacatttt
    45601 ttcgctattt gtattcattt caattccctc ttatctcact gacgatttgt tgaaataccg
    45661 tcattgttct caataattct ttttcgtttg gatgttggta tccgtctatt atacctattt
    45721 tttctaaaac aagtattttt ttgctttttt ctgccaaaaa aagaactttt tccaaatact
    45781 ccatctttga aatctgttcc agccagtgga ttggatagcc aagcaagtcc agccacacaa
    45841 aaaagttttt tagttggaaa tcaaaaggat atgcttggag attccacacg aaatgaaaca
    45901 tcattcctaa aaattttttc atactagcag aaagtagatg gccattttcg gtcagataaa
    45961 aagcttgttc aaacagttta ccataatttg acaggttttc tgcctctaat tgataaaaat
    46021 agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt taattgcttt ttatttggat
    46081 agtttttata cagattttgc aaaaaacgat gatcacaaat cattccgcaa cagataaaag
    46141 tcaaaaagtc aatctgcttt ccgtccacct gccgctcgct gattgtttga tcataaacga
    46201 tgcgttctgg cagattgatg gtttgcagca ctacacgatt tggtagctgc aaaatcgtcc
    46261 gctgcggtgt taatgctcgg cttagagaac ggatcgatac agggatcatc catagtgatg
    46321 aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc catgatccct tcatttccaa
    46381 atccgatgat aaacatggat tgattttcgg gatagcgttt gacgaaatca agcaggttct
    46441 ttaattctgt taaatgattg cattgagtgt tggcacaaat ataccagtca ttgtttgctt
    46501 gatttgcaaa taacgggttg atcttatctg caaacaaatc ataatagcgc tgattcgtta
    46561 aaaataagat tggctgatca atggaaagtt ctcttagctg actgactagc gtttctccgt
    46621 aaaacacttt cgtcaacgca tcttctactt gataatcaag ttccatacgc atccctcctc
    46681 ttcttatttt tattttacca ttagtaagca aattctagaa aagataagag caaagctcac
    46741 ctcataactt gcaaataaat aaaaattaaa taaaatacat gtgttttcta tgtatgacat
    46801 acggattcgg aatctctgtc tcttatttat tccttttctg ctaggaaata aaaagaaaag
    46861 cacttgtagc cctagagcca caagcgcttt ttcaactgtc ttatttaaca gcgtctttta
    46921 atgctttacc tggtttgaat gcaggtactt tgctagctgg aatttcgatt tcttcaccag
    46981 tttgtgggtt acgacctttg cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga
    47041 ttaattgaac tttttcgccg ttagctaaag catcttgaat agttgaaaat acagcatcta
    47101 ctgctgcagt tgcatctttt ttagttaaat cagtagctga agcaactttt tcgattaatt
    47161 ctgctttgtt tgccatggat agattcacct cctccacaag agatatgata gaaatatcat
    47221 ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga aatattgatt aacttcaata
    47281 ttctgttatc acgatatcat atgaaccgct taatagcaag gtttttacca attttcacat
    47341 gcttttttta gtcatagaaa cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac
    47401 gtcgatatgg taaaatgcct attttctcct acgagggata attcggatgg acgtcccttc
    47461 aaaagtaaat gcttttctga tttgattttc taagaaacgt tcataagaaa aatgcatcaa
    47521 ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg acagcgactt gtgtagcata
    47581 aaaaatcttc aggcgttttc ctttatctgt tggtgttgga ttgattgcta cagcatccat
    47641 aatgatatca ttcaacacag ccgaagagat acgaagattc tggttcatac ttacttgttc
    47701 gatcatttca ggcagacgtt ccaaccgttg tttagtcact gcagaaacaa aaacgattgg
    47761 agcgtaatca agataccgga attcatcacg aatctcttgt tcgaaatcac gcattgtatt
    47821 ggtttctttc tttacaagat cccatttatt cacgacgata ataattccac gaccagcttc
    47881 atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc ccttcttcag cattcagtac
    47941 catcaaaacg atatcagaac ggtcaatggc acgcattgca cgcatcacgc tgtatttttc
    48001 agtattttcg tatacttttc cgcgtttacg cataccagct gtatctatca ttaagaattt
    48061 ctgaccactt tcagaaacga agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc
    48121 cgaaacaatt acacgttctt ctcctaaaat cgcattgatc aaagaggatt taccaacatt
    48181 tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga
    48241 gaaatacttc actgcttcat ctaaaatatc accaatccct agtccatggc ttcctgaaac
    48301 aggataagga tcgcctagcc ctaaggaata aaattcatag atatcgtttc tcatttctgg
    48361 attatctact ttattgactg ctaaaatcac tggtttatta cttctgtata aaattttcgc
    48421 aaccatctca tctgcgtctg tcacgccttc gcgtccgctg actacacaaa tgattacatc
    48481 tgcttcttca atcgcgattt ctgcttgatg cttgatctga tccataaaag gttcatcgcc
    48541 aagatcaatt ccgcctgtgt cgatcacgct gaattctcga cctaaccatt caccttttgc
    48601 ataaatacga tcacgagtta ctccaggagt atcttcgaca atcgagatac gctcaccagc
    48661 gatgcggtta aaaatcgtag acttaccgac gttcgggcgg ccaacgattg caattgttgg
    48721 atttgccata ttatttcctc ctcacaagtt tccaatctca cttagtttaa cgaatgcatt
    48781 gccaactttc aagcatagat tgtcaattaa acgaagtatt tttattcctt attgaaatac
    48841 taatcgcttt tcaaaagaaa aactgagaca aaagcagctg ctttcatctc agtcacagat
    48901 acaaaatcaa ttatttttca gaatcatcgg ttgctttttc ttctacttct tctttcaacg
    48961 catcacctaa aatgtctccc atagtaaatc cagtattttc ttctggtaat tcataagact
    49021 caacattttt tggttcttct tctgaagctg gtttttcttc taaagctttg atactcaatg
    49081 caatacgatg ttcttctgga tggatctcta atactttcac tttcacttcg tcaccttcat
    49141 gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat ttgagagata tggactagcc
    49201 cttctactcc agggaacact tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc
    49261 cgtctaatac tgtgccaact ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg
    49321 tgtctttgat tgacaaggaa acacgttctt gttcaggatt gatagacaac actttgacat
    49381 ttacatcatc attgacagcc aatacatcac ttggttttgc tacatgacta tgtgaaatct
    49441 ctgagacatg gactagaccg tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa
    49501 gacgtgcaac acgtccactg agaacatctc cctcatggat agttgaaaga atttcttttt
    49561 tcttttcttc tctttcgttt tccactaccg ctttatgcga taagatcaaa cgattttctg
    49621 atggttcgat ttcgatgatt ttaaagctca atgttttgcc tttatagtct gaaaagtctg
    49681 caacaaaatg atcttccacc attgatgcag ggacaaaacc acgtacaccg acgtcaacga
    49741 ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat gaccttgccc tcttggaaat
    49801 ctctttcgat atcttcccaa acttttttag catctaaacg gcgttttgaa agtagatagc
    49861 tgccgttttc tttatctttg ccgatcgtac tgataacgac taaatctaat acatcaccga
    49921 cttttactac ttcgctgata tcttctaccg gcaaagttga taattctttt gccggaacga
    49981 ctccttcaac tccagcccct tcgattccga caatcacttg tttgtcttca atgactaaaa
    50041 cttcgccctt aacaacatcg ccaactttta cttcttgtac gcttttcatt gcgtcttcca
    50101 ttgactggtt gttctcttga ttttgatcaa attctgtcat aacacttgtc ctcctaacca
    50161 acattctgcc gtaagtacgg tactgttttt agtttaattg attattcaaa aaaaataaag
    50221 cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca ttttttttct taatttaaaa
    50281 aagaagataa ccttttttca aaacaacagc ttttattgct gcaacgactt ctggaatcga
    50341 tttgccagtc gtatcgatac gaacagcatc ctctgcttgc ttcagcggag agacgtccct
    50401 tgttgaatca taatggtctc tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg
    50461 aattcctttt tcttggttct ctttgaaacg tctttctgca cgctcactta cgcttgctac
    50521 taaaaagatt ttcacttctg cattcggtaa tacagctgtc ccgatgtctc ttccgtccat
    50581 gacaacgcca ccgcgttccc ctatttttcg ttggacagca actaattctt ctctgacttt
    50641 tccatgtgca gagacttcag aaacagcttt tgtgacatct ggttgacgga tttctaaagt
    50701 cacatctttt gatgctatga atacatgctg tccatcttct tgttgcgaaa aagagatagg
    50761 atactttttg atcagcgaaa ctagcccttc ttcatctgaa aaagatacat tgttcgtaat
    50821 agctaaataa gttactgcgc gatacatggc accggtatct gtataaatat aattcaattc
    50881 ttttgctaat atcttggcaa cggtactttt cccagatgaa gcaggcccgt cgattgcaat
    50941 actgatttgt gtcatgatat aactcctttt tctcagaaaa aaagccgcaa agcgcggcta
    51001 ttattattta attcttaatt cttgaccagg gtaaagcata aagttgtttg gatctaagcc
    51061 attcaattca aacaattgat cagtggtgat ccctgcacgc tcagccactt gttttggacc
    51121 ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc
    51181 tgctgcattg ctgcttgaat cccctgcttg ataagttgcc tcacttgagg aagtcggtgt
    51241 tgtctctgct gcaatactgc tttctgacgt tacacctgta tcttgagaca cagtagattc
    51301 ttccgcgatt gtactagatt ctactgttga agtctcagtt gactcctctt ttgttgtact
    51361 tgtagaactt tctactactg atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt
    51421 gcttgatcca tttctgatcc agaaataagc acctgctgga atagcgatac ataagaccag
    51481 taaaatcaaa aatagcgaaa gaaataatgt atttcctttc ttttgttgtc tctgagaact
    51541 tcttgatgaa acttcatcat ctgtatcgta gattggttgt tcccatggtt cttgtgtttc
    51601 gttattttct gtagattgat gtctatcttt tctgctcact gttttacctc ctaaactcta
    51661 tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt
    51721 tttttaaata aatttagtaa aatcgttccc caatgaagtt gttcagtctt agaaaaaagc
    51781 ttcctctttg gcagaagttc tgcttttgct ccatcaatat cacaacaatt ctgcggcttt
    51841 tcggagagtt tttcgccaaa ataagctaag atgaattttc tgcggcagtt ttcttcatta
    51901 atatagcgaa gcatctgctg caggcggaat tctttttctt tttcctgacg tttgagccgt
    51961 tccacccaat tttctggttt ttcagactgc tggatcagct ccatccattt tttttgcagc
    52021 tcatcaaagg gagcttgctc tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt
    52081 tctctagaaa gttgattaaa gaaataatgg atacgttcat ccccactttg ataaaaaaga
    52141 attgctgcac tttcttcctg atctctgcct gctcgaccga tttcttgtac atagttttct
    52201 agactgtctg gcaaatcata atggaccaca taacggatat cagatttatc gatccccata
    52261 ccaaatgcat tcgtagcaat gagaaactgc agctgatttt ttacaaactg ctgttgaagt
    52321 tgtcttcttt gagctgcatc aagaccaccg tgatagtatc caactgtaaa acgttccctg
    52381 aataaatgat acaatcgttc gacttctttt tttgttgcac aataaatgat tgccgctcct
    52441 tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa
    52501 agcgagatat tttgccgatt gactgattgt ctgatctctt ttggtggtcg atcaaatagt
    52561 acatgacgta tatctcttgc tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc
    52621 gggtttccta aaagctttcg gatttttcct aactgttgat attctggacg gaaatcaact
    52681 ccccattgag acacgcaatg agcctcatct actacatata acgcaatatt ttgttttttc
    52741 agatgctcta aaagagaagg attagtcaac atttcgggac tcaaaaacaa aaatttatat
    52801 tgcgatagat gacggaggac gtatcttctt tcagattgcg aaagtaaact attgaaagca
    52861 gctactcgtt tctcccctcg tttttgtaat tgcgtcactt gatcttccat tagagaaagc
    52921 agcggtgaca cgatgatgac cagaccttcc attaaatacc ccgtcaattg atagcaaagg
    52981 ctttttcccg ttcctgtagg caaaatagcg agagtatctt ctccatcaag caacgcctga
    53041 ataatttctt cttggccggg acgaaatgaa tcaaatccaa aatatttttt taattcattt
    53101 attaacattc tgtatcgctt cctttctcaa tagatagatt tgatacaaac gaaattctcc
    53161 ataatctaat tgtccatcta cattccattc gctaaagcgc cactctcgca cggaatcctt
    53221 atttggttcc aaacgttcga ttgtttctgc agagagaatc cactcgtatg gaaaatcatc
    53281 aaaaaacaag gcccactcaa taagatgatc tgttactgtt cctcttttta aatgacggat
    53341 tgccatgatc tggtcaattg tctgaccatt cataaacatc tgcttggtga tcatcatgct
    53401 ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg tgccaaagcg aatctggccg
    53461 tttttcgatt tgtgctaaca aaaggtggat acatctgctt tgttgcagat aaaccgctga
    53521 ctcatcattg ttttttgcca gctgataagg aagcagccct gttcgctcat ttccagataa
    53581 ttggttagct aaaaaatctg ctgcttcttc tggtatctcc gaaaacagct gcaccaaact
    53641 ctcatacgca gtatcaatca aaatttcacg tggcaattgg ctttcactaa gccatttttt
    53701 tagttggaaa gtataaaaag gacttgtctc tgctggaaga tagtcttgaa tacccgttgc
    53761 cagattagaa attacttgaa cagcaaattt aatgagccgc caactagttt cccctgttct
    53821 gccatagcgg tcaaaacgca aaccagtatg ttctatttga tgttcggaca acatatcagc
    53881 ccctgcaggc gttagctttg cttcattttc atttatttcg atccaacctt gctgcgcaat
    53941 tttttgcaaa atttgataaa acttatcctg cttcaattct ggaaaactgt tatggataaa
    54001 caaaagttcg tgggtaaatc cataaataag tactgaagtc gttcgttttc ccacaaggag
    54061 ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa aatagcgata aaataaaaac
    54121 aatcatatta ttctccttaa gttaggatgg ttagaaatgt cactactatg taaacttgta
    54181 cctgaacgct gtattgcatg cggtctttgt caaatcgaag cgccagatat tttcgattat
    54241 gatgaagaag gaatcgtttt atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg
    54301 ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta
    54361 ttagaaaata aaatataaat ataaacgaaa ctttaaaaac gtacactaag ttctctaaac
    54421 ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga
    54481 ttcatttgct atctatagtt aattgactag ttttttcacg gaccctatag gacccattta
    54541 tattatttca actttttcag ccagttttcc aatgtattga cgatcacagt ttgctggtct
    54601 gcgttagaaa tttctgcctc tccatctcct ttttgctcac catagctgcc aaaacctcca
    54661 tgatttccgc cattgattga tacatactct gttctttccg gtaaatactg cttagcttct
    54721 tgataagctt cctgattcaa tactttatct tttgtcgccg tgatcgataa aacaggaaca
    54781 gctgattctg tcaatgttcc ttttttgtcg ggataacttg ccatgaagaa tacccctttt
    54841 aaatgtttgt ctgaatgatt cttattgaca taacgacttg ccattacgcc acctaatgaa
    54901 tggccaccga tcacataatc ggaatttgga aaatcttctt tgaccttatc cgctttgttt
    54961 ccgcccatca ctgctagatc aagtggcatt ttgacaatag cgactgaaaa gccgtttttt
    55021 gctaaatctt gcgcccaaat actataactt tctggctcta cgagagcacc aggataaaaa
    55081 ataagtaatg gagactttcc atgattcttt ttctctcctt caaaaaataa gtaatcttct
    55141 ttgttttttc cttcttttac caacacttgc gcttctgacg atggttcgta cgtattttgc
    55201 tgtatatagt aataaccggc tccaccgagt aatcctaaaa tgatgactaa gccaattagt
    55261 attcgtctaa atattttcac tttctcccac cttttctatc tgagtgaccc ctttttctta
    55321 atcaagtatg attatacctt aaaaaaataa gagagaaatg caaactttcc agcatcaaat
    55381 aaaaaaagac ggaatatcca tttgaatatc cgcccttttt gtatgtttat ttttatttag
    55441 cacataaacg atacacagct tctgcataaa tttccattgc tttatacata ctacttaatt
    55501 cccatcgttc attcgcctgg tgcataaagt caggagtatc agggaacatc gcaccgaatg
    55561 ccacacactg gttcatcgta cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca
    55621 tgtctccagt aatatcgcga tatgtcccca atagattttt cactaactca ctgtctagtg
    55681 gcacgtataa tgaatcaagg aagtcaaatt cttcataagt caacccatat ttttctgctg
    55741 ttttagttaa tttagctact aaatcatctt tcttgaatgt aacagggatt ctcatatcta
    55801 caccgatttt tgtttgttct ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc
    55861 cggattgttc atcttctgtt ttgcccacga cagcttcacc agttgcattt tcttgaacta
    55921 attttcctaa gaaattgatt gggccaaaat caaatacttc tgaaagagca attgccagac
    55981 gagaaatagc attcactcct tgtgctgcat ctttcgcgtg gatactttta ccaagaacga
    56041 cgatgccttc accttgttct tcgaaatcga attcgtgttt tttcaatgct tcttttactt
    56101 cagaaagctt ttctccagag taaggagctg catcaggaac aacattcaac gctcctccag
    56161 cttttacaga aaactcgttc gttcctggtc ctgtcaagta agcttgcaat aagccttttt
    56221 ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt
    56281 tttcgttata tttttctaaa caacgccata aattttcttc atctgtacca aagataaaac
    56341 gaatacgtgt cttgaattcc acgccagcat caatcaaagc tttgacggca tacattgccg
    56401 caatagaagg acctttgtca tcttgggatc cgcgaccaac taaccagccg tcttttatag
    56461 ttggatcgaa tggttttgtt tcccaattat tttcgtcccc tgctggaaca acgtccatat
    56521 gacacaagat accgaataat tcatctccag agccaatctc tgaataaccg taatagcctt
    56581 ctggatcttt aaatgttcta aatcctagct tttcactgat ttccaatact ttatccaaag
    56641 cttcaatcac ttttttaccg aagggatgtc cttgtcctga atctgcttca tctagtacgg
    56701 aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca
    56761 caaatttttt catgatatta tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc
    56821 gacacaacct cttcttctca aataatgacg ttttaagcct gctccatcag caagcagaat
    56881 gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg gagctattga cttatccacg
    56941 tgcttatcga tagccgttta tcggtttctt cattcttatg atagaccttg tacctgtatt
    57001 tgtctaggtt tagaacattg ctgctacacc taggatcaca cagatggcta caaataatat
    57061 cgcaattaat ttaccggtaa atttccacca cactgaaaca tcgaaacgag caattgctac
    57121 agcacccatt acgatagcag atgttggtgt aattaagttt actaaaccag atgcagattg
    57181 ataagcagtg attactaaat ccttaccgac gccggcaaat tcacccatag gtcccatgat
    57241 tcccattgtt gcagcagcta agccggaagt tgaaggaatc aagaatgaca tagggatata
    57301 gaaaataaat gtaataatga tgaatacact tgaagataat gaacttaatc cttgttcgcc
    57361 ccaatgtaag atggtgtctg tgattaatcc attgttcata accacttgga taccacgagc
    57421 aacagctact acgattgcta caccgatcaa atcagaagca ccagctaaaa attcagagac
    57481 aaattcagac tctttcatgc cataagtgat tgctactacg atggacatta ctaagaataa
    57541 catagtaatt tctgggaagt accagtcacc taatggaagc atgcttttac ctagaaccgt
    57601 acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc
    57661 attcaaggtt gtccaaggaa tcaaactgat gatcatgata ccaaaagtaa taaagaacaa
    57721 tacattaaca tgtttttgtt ttttcgtgat tacttcttga cgaccgattg cttcaatatc
    57781 gaaatgcttc atatcttctt tacgttggtt gtatactaat gattttgttg gatctttttc
    57841 gatctttgat gcataacgat acacatatac aatagaaata gctaacaaaa taacgaacat
    57901 caagaatcga agaccaatac cttcacccat cgaaatccca acagcttgtg aagctacgcc
    57961 tgttgcaaaa ggatttaccg ttgaagctag acagcctact tgagacccta ctaatacgat
    58021 tgctacagct actactgtat caaagcctac agccatcatg actgggatca gtaaagcata
    58081 gaatggaatc gtttcttctg ccattccgta agtagagccg cctaatgcaa ataacagcat
    58141 caatactgga ataagcattt tttcttttcc tttgaatttc ttcacaactg aagcaatccc
    58201 ggcatctagt gcccctgttt tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa
    58261 gaaagagatt gggattgcag caggtgtttc tttagttagc tcccccgctg gtacacctag
    58321 catcccattg attggcgcca tgaaaatatc ccacagccct tgaggatttt gttgtactcg
    58381 ttcatatgat ccagcgacga tgttgcctgc atcgtctaca ctatagtgcc cagctggtat
    58441 aaaccaagta agtacagcga ttactgcaat aataataaat aagaccgtat acgctgaagg
    58501 catttgaaat tttttctttt ttgtttgttc catcttcttc ttcctttctt ttccgttaga
    58561 gacaacaatt ttggctctaa tgttatttta tcaaaaagag tatcagaaca aaaataataa
    58621 cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt ccctattccc tcctcttttt
    58681 tcaggttttt aaagctttct tctttttcat tgtagcgttt cctttttttt ataaacaaac
    58741 attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt gatttttatg
    58801 cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt gcatgtcttg
    58861 tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat ttatttgact
    58921 gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag aagttgcgca
    58981 tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa aggaacgatc
    59041 ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt cagccctaaa
    59101 aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa aatagttaga
    59161 gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg tttctttaca
    59221 ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact agcagtgtct
    59281 cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga acgcaagaac
    59341 gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac cgagatatca
    59401 ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa
    59461 actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg ggtgttcttc
    59521 atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt ctctccaaat
    59581 gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa
    59641 taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac cgattcagct
    59701 atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca ccctgcccct
    59761 gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta ttcaaaaaac
    59821 gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc aaactgctga
    59881 cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa gtcagatctc
    59941 cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc atatttttca
    60001 cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt gtcttaggat
    60061 ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc tttattcctt
    60121 ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca acgtaaactt
    60181 tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca tttgtcagta
    60241 gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt tcttttacca
    60301 tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca gaaataactc
    60361 ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg acttcatcca
    60421 cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact tgtccgttta
    60481 cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg atgccagcgt
    60541 gggcaattac tttctgcaat ctctccatta caattattct cctttattca ttccatcaaa
    60601 gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg tatcaggcag
    60661 ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc catataatat
    60721 cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg cagctagacg
    60781 ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc ttgtgattgg
    60841 ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg tgtttgatgt
    60901 cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta atacataatg
    60961 gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag attcttttaa
    61021 ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc ttaattcttc
    61081 attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac ttaatttcgt
    61141 catgttcggt atctcctact gctggataaa gcataattga ttcatagttt tctttttgtt
    61201 gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta gtgacaactt
    61261 cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca tttcttttta
    61321 tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt
    61381 tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca cggatcaaat
    61441 cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac ccatcgatgt
    61501 tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc agtctttcag
    61561 ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct cgaggatctt
    61621 cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat gtcttgcttt
    61681 tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct agctcgagtg
    61741 ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc ggtatatcat
    61801 atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga ccctcgaata
    61861 catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt tctttgctag
    61921 ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat ccgggtactt
    61981 ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag acggattcaa
    62041 agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa taatcccttg
    62101 aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt cagattcgta
    62161 cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga aactcaatag
    62221 tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct caccttccta
    62281 tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct tcgtgatatg
    62341 tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta ctgtccgcaa
    62401 atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac
    62461 ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga ttccttgccg
    62521 agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt cctttacaag
    62581 taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat cacctaaagg
    62641 aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag aaagatgaag
    62701 atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat acatgacttc
    62761 caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt caatcagctt
    62821 ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg gcgtatcgat
    62881 atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat ggaatcttct
    62941 gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat gcaacttctc
    63001 cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt tctgctcctg
    63061 tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa
    63121 tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca taataatccc
    63181 cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt tccataatct
    63241 gagcaaagaa aaagccgtct agacactcag acggactggt ttcttttttc tttttcatag
    63301 cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa atgataacgt
    63361 tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc gatcgtgccg
    63421 cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct caaatcataa
    63481 cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt caagatttca
    63541 agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac taaaaagaag
    63601 atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag agtagcttct
    63661 ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt tttcatcgcc
    63721 gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta caaataaaaa
    63781 aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa catgcaaggg
    63841 attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta atcaatcctt
    63901 gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca aaagctttca
    63961 tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca gaacgttcta
    64021 aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt ggcttcaatg
    64081 aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc tgctcgccta
    64141 gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa tcttccgtta
    64201 aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct ttgtttttca
    64261 tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct aaactgcccc
    64321 aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct tttttaggcc
    64381 atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta tctggaagac
    64441 cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca aaagcgtaat
    64501 gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt tgattttggt
    64561 aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg cttaagccaa
    64621 aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata ataatacctg
    64681 taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa aggttttctt
    64741 aatacatttc tttgctatca taacataatg ttatctactt gaatagctga gccttggttt
    64801 ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt tcgaataaac
    64861 ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg ctcccatttc
    64921 ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa gcactttctc
    64981 caatgccttt catcaatgca gattttgttg aagtggcata tagattataa tagccttccc
    65041 cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat ccacgttgga
    65101 tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct ggattagatg
    65161 catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag tttgaagagg
    65221 aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg
    65281 cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct gcttttttcg
    65341 cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg agcatcttat
    65401 ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc actttttcta
    65461 ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact tttccgtctt
    65521 taaagactat agctgttttt tctttagctg tttgcaattc agcatattga tttttagctt
    65581 ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg attgcttgga
    65641 tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg atggcaggtg
    65701 ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata tcttggtaag
    65761 ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca ttttttagtt
    65821 ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca tctgtataaa
    65881 aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct tctgctgcta
    65941 cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca ccgagtagaa
    66001 gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc gctttatctg
    66061 gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc acgattgtta
    66121 ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct atttttgctt
    66181 caagatcagt accctctttg cttacagatt gattttcagc atcggattca ctatttattt
    66241 catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg
    66301 gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca gttttttctg
    66361 tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg ttttctctat
    66421 cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc tgtgcaattt
    66481 tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct tcacgatgtg
    66541 ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt tctttcagtt
    66601 cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt cctgattctt
    66661 ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta gttacttgat
    66721 cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc attacagatt
    66781 ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct gtatcaggaa
    66841 agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt tcttcaattg
    66901 attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga cagatagtat
    66961 tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag cgatcggctc
    67021 ctttactagg tttacgctac catttaatct atcatattct atacgtcacg ccaatagctt
    67081 atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa aaaaagcttg
    67141 agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt tactgatttc
    67201 tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt gaacgagaag
    67261 gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc cgagtaacac
    67321 caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta
    67381 agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc aattgttctt
    67441 ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct tctatgaaat
    67501 cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg
    67561 cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca ccgatttctt
    67621 ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt atcaatttat
    67681 taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca gcgattgctc
    67741 gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat cctttgcggt
    67801 agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga
    67861 actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga aggtttgctt
    67921 ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct aacgctactt
    67981 cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata cgaaccgggt
    68041 cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt
    68101 tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc acactgatac
    68161 ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg
    68221 tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg ttttctttga
    68281 tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc ataaaagaag
    68341 gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga tgatttcaat
    68401 cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct gcatttgttt
    68461 ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca agtcttggat
    68521 ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca ccaaccgcct
    68581 taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga
    68641 gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa actgaaattc
    68701 tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata atagaatctg
    68761 ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac
    68821 ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc ttcgaagatc
    68881 gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca gttgagatag
    68941 atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta attcttcgag
    69001 gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat aacgtgattt
    69061 aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg cttctccata
    69121 cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag aaagtgctaa
    69181 tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc
    69241 tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga tctgttgagc
    69301 tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc atgcagcaat
    69361 cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat cgcttcttgc
    69421 tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg gactattcaa
    69481 gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc
    69541 gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa aacgatcaga
    69601 catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt catttgaaat
    69661 tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt
    69721 gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta aagctggctg
    69781 tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt catgagcagc
    69841 aatcagtttc cccgtagcag attcaggatt tcggacagct atagtacgct gacgccattg
    69901 atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg gaaaacttag
    69961 accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac caaagcaatg
    70021 aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat gaaaaggaca
    70081 cagccccaag taatttttac cagacttttt caactggaca tattgtccaa tcacctcaac
    70141 gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt gagccatttt
    70201 ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca ggagaataac
    70261 cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat tatacatgtt
    70321 tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc attaagcatc
    70381 aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac aatactcgaa
    70441 gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc aaccctaagt
    70501 ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg atcgtcgtta
    70561 ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact gccatgcaac
    70621 tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc catgcgaatg
    70681 tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca tttggtaaag
    70741 cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct tgttcaaaag
    70801 ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt tggataggga
    70861 taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat gaataaatcg
    70921 ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg cgtccttcaa
    70981 acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc gcaatctgac
    71041 gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa
    71101 catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc aattcattta
    71161 ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga tgagcagctt
    71221 ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca taattcggta
    71281 atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa atcactttca
    71341 gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt cggattggat
    71401 catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg ttcaattgct
    71461 gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct gtttttagac
    71521 tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac acgactccca
    71581 gttctggaaa ttccatcgcg ccccatttgc gatcagaaga aaatgcgact ttgtttgtca
    71641 catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg atgttgttat
    71701 cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg tacagcggat
    71761 gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct gttttatcca
    71821 agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga acaaggattc
    71881 gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc aataccaaac
    71941 cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt ttgatagaag
    72001 catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata atcacgaaac
    72061 tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata gctttgtgag
    72121 tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg ataacttcag
    72181 ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca gtttctttga
    72241 caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt ccagatataa
    72301 cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct cctaatacca
    72361 gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact tctgattggg
    72421 aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa attccaataa
    72481 cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct acaaataaca
    72541 gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta tcgctgaata
    72601 tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca gaagccattt
    72661 tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt ttccgccaac
    72721 tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac aacttgtttt
    72781 tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa cctttttgtt
    72841 tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg ttcctccaaa
    72901 cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca agctagtacc
    72961 ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga agcaaaaact
    73021 acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta atccatgatt
    73081 tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat cacttcaagt
    73141 cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac caaaaaatta
    73201 gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc aatcagccat
    73261 gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa taaaaacaaa
    73321 taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct tccttttttc
    73381 cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa aaaccataat
    73441 atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag aaagatgcct
    73501 gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa attttttgta
    73561 aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc taatgcacca
    73621 ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac
    73681 atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct tttttccact
    73741 atttgcgtac tccttctttc atttaatttt attcatgatt gaacatttcc tgctatttta
    73801 cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa tatcaggata
    73861 aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg actggatccg
    73921 ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt gggctcagca catctttaga
    73981 atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat aggcaatata
    74041 atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt gtttctttgc
    74101 ttctactatc atttccgcca aatatttgac accttgatca atactgtcag ctggattatc
    74161 aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg tcccttttcc
    74221 ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca tatgataatt
    74281 ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg aacgataagt
    74341 tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat
    74401 acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg acatcttttt
    74461 ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt gtacgcctta
    74521 agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt tgtttttcag
    74581 taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag gtgattatgt
    74641 gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact atgaaaagag
    74701 cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc gctttttttc
    74761 agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata gccgaggaca
    74821 tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg aggatctgaa
    74881 tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta gtgatggagc
    74941 aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa
    75001 ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca gtaatattca
    75061 ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa atcttttagt
    75121 tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa aaatcaactc
    75181 ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat
    75241 agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac atgaattagc
    75301 tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg aaacataact
    75361 attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg aaaaaccacc
    75421 taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc ggtgaattga
    75481 tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc actatacctg
    75541 tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct atggttggtt
    75601 atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga
    75661 caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt gttagtgtgg
    75721 ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc caagcattat
    75781 cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt tcttttttac
    75841 ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg ttgatcggcg
    75901 gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa ggattccttg
    75961 gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg gaatggaccg
    76021 gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt gatctgtggc
    76081 aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg attccaggag
    76141 aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt gtgccaagag
    76201 aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt ccgtttttga
    76261 ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt tattctggta
    76321 tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta ttatactttt
    76381 ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag tttcgctggc
    76441 ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt atactattgg
    76501 gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc
    76561 ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat tttagtttca
    76621 caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa aacgaattat
    76681 ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc attattggtg
    76741 cactgtcctt gctttacatc gttattgggg tatacaatct gccggatttc cctcatcggc
    76801 gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt tcgggattgt
    76861 tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt caaggagaaa
    76921 agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt acaacttatg
    76981 gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt gcctatgaaa
    77041 aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa tgtattgtaa
    77101 tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct tttatcgaag
    77161 aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag attgttatga
    77221 ttttgcatga attcggctac gatttcatta agatggggga agaagcttat gtagacctga
    77281 atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg aatcggatcg
    77341 aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag caaatgagta
    77401 ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg ttttctttag
    77461 gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa aatacccaag
    77521 aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa gtaggaacga
    77581 ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat ttcttgttct
    77641 tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta gggatggccc
    77701 ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt gcatacttgg
    77761 tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat aaaagcaagt
    77821 acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg attgcatatg
    77881 ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa ggtagaataa
    77941 gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat agatgaacac
    78001 tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta caatctcttc
    78061 ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac cgctctttta
    78121 ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt cgatctctgt
    78181 aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc
    78241 aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat
    78301 ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga agtagaactg
    78361 tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg tcaacacgta
    78421 cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa gtacttgtcc
    78481 acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat ccccagcttc
    78541 cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt tttgtgtttc
    78601 aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac gatcaatacg
    78661 acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg gcaatagtaa
    78721 tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat ccatcaattc
    78781 cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat
    78841 cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa cttccatttc
    78901 taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga aaacaatcag
    78961 gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag gcatcggacc
    79021 gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg taatcatgtt
    79081 tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc
    79141 atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg gagctgcatc
    79201 aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg tagtgatcgc
    79261 agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg
    79321 tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat aatatagatc
    79381 actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa atcaaaaaga
    79441 atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc aatattggtc
    79501 aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt tataaataaa
    79561 aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg aagcaataag
    79621 aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat agacgaaatc
    79681 ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact
    79741 tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt tttacggcgg
    79801 cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc aagcagataa
    79861 cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag atggtgaaaa
    79921 aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg cgtatcttaa
    79981 actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg cttctgaagt
    80041 gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat tttattagat
    80101 aaaa
//
LOCUS       DAZKCE010000002        68691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000002 DAZKCE010000000
VERSION     DAZKCE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.002"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8KQ_000076"
     CDS             complement(132..509)
                     /locus_tag="ACG8KQ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103055.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8KQ_000077"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8KQ_000077"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9103056.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8KQ_000078"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8KQ_000078"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9103057.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8KQ_000079"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8KQ_000079"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9103058.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8KQ_000080"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8KQ_000080"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9103059.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8KQ_000081"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8KQ_000081"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9103060.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8KQ_000082"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8KQ_000082"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9103061.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8KQ_000083"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8KQ_000083"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9103062.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8KQ_000084"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8KQ_000084"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9103063.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8KQ_000085"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8KQ_000085"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9103064.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8KQ_000086"
     CDS             10661..11575
                     /locus_tag="ACG8KQ_000086"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9103065.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8KQ_000087"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8KQ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103066.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8KQ_000088"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8KQ_000088"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9103067.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8KQ_000089"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8KQ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9103068.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8KQ_000090"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8KQ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9103069.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8KQ_000091"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8KQ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9103070.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8KQ_000092"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8KQ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9103071.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8KQ_000093"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8KQ_000093"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9103072.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG8KQ_000094"
     CDS             21340..22764
                     /locus_tag="ACG8KQ_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9103073.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG8KQ_000095"
     CDS             complement(22806..23063)
                     /locus_tag="ACG8KQ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103074.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8KQ_000096"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8KQ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9103075.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG8KQ_000097"
     CDS             24337..25314
                     /locus_tag="ACG8KQ_000097"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9103076.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACG8KQ_000098"
     CDS             25339..26982
                     /locus_tag="ACG8KQ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9103077.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG8KQ_000099"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG8KQ_000099"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9103078.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACG8KQ_000100"
     CDS             28558..28833
                     /locus_tag="ACG8KQ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9103079.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACG8KQ_000101"
     CDS             28839..29606
                     /locus_tag="ACG8KQ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9103080.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACG8KQ_000102"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACG8KQ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9103081.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="ACG8KQ_000103"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="ACG8KQ_000103"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9103082.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="ACG8KQ_000104"
     CDS             33750..34052
                     /locus_tag="ACG8KQ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9103083.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="ACG8KQ_000105"
     CDS             34055..35218
                     /locus_tag="ACG8KQ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9103084.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="ACG8KQ_000106"
     CDS             35244..38672
                     /locus_tag="ACG8KQ_000106"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9103085.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="ACG8KQ_000107"
     CDS             38693..39817
                     /locus_tag="ACG8KQ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9103086.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39798..40244
                     /locus_tag="ACG8KQ_000108"
     CDS             39798..40244
                     /locus_tag="ACG8KQ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9103087.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="ACG8KQ_000109"
     CDS             40228..40566
                     /locus_tag="ACG8KQ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9103088.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="ACG8KQ_000110"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="ACG8KQ_000110"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9103089.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="ACG8KQ_000111"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="ACG8KQ_000111"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9103090.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="ACG8KQ_000112"
     CDS             41723..42766
                     /locus_tag="ACG8KQ_000112"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9103091.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="ACG8KQ_000113"
     CDS             42925..43653
                     /locus_tag="ACG8KQ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9103092.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="ACG8KQ_000114"
     CDS             43769..44014
                     /locus_tag="ACG8KQ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9103093.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="ACG8KQ_000115"
     CDS             44011..44229
                     /locus_tag="ACG8KQ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9103094.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="ACG8KQ_000116"
     CDS             44365..44904
                     /locus_tag="ACG8KQ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9103095.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="ACG8KQ_000117"
     CDS             44897..47170
                     /locus_tag="ACG8KQ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9103096.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="ACG8KQ_000118"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="ACG8KQ_000118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9103097.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACG8KQ_000119"
     CDS             complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACG8KQ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9103098.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="ACG8KQ_000120"
     CDS             complement(48997..49266)
                     /locus_tag="ACG8KQ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103099.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49528..49656)
                     /locus_tag="ACG8KQ_000121"
     CDS             complement(49528..49656)
                     /locus_tag="ACG8KQ_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103100.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49736..50191)
                     /locus_tag="ACG8KQ_000122"
     CDS             complement(49736..50191)
                     /locus_tag="ACG8KQ_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9103101.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="ACG8KQ_000123"
     CDS             complement(50329..52257)
                     /locus_tag="ACG8KQ_000123"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9103102.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="ACG8KQ_000124"
     CDS             complement(52454..53287)
                     /locus_tag="ACG8KQ_000124"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9103103.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="ACG8KQ_000125"
     CDS             complement(53479..54183)
                     /locus_tag="ACG8KQ_000125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9103104.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="ACG8KQ_000126"
     CDS             complement(54206..54832)
                     /locus_tag="ACG8KQ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9103105.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="ACG8KQ_000127"
     CDS             55158..57533
                     /locus_tag="ACG8KQ_000127"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9103106.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="ACG8KQ_000128"
     CDS             complement(57557..58048)
                     /locus_tag="ACG8KQ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9103107.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="ACG8KQ_000129"
     CDS             58235..59155
                     /locus_tag="ACG8KQ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9103108.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="ACG8KQ_000130"
     CDS             59309..59653
                     /locus_tag="ACG8KQ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9103109.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="ACG8KQ_000131"
     CDS             complement(59843..60343)
                     /locus_tag="ACG8KQ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9103110.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="ACG8KQ_000132"
     tRNA            60871..60954
                     /locus_tag="ACG8KQ_000132"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..61916)
                     /locus_tag="ACG8KQ_000133"
     CDS             complement(61278..61916)
                     /locus_tag="ACG8KQ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9103111.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            62251..62685
                     /locus_tag="ACG8KQ_000134"
     CDS             62251..62685
                     /locus_tag="ACG8KQ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9103112.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            62708..63028
                     /locus_tag="ACG8KQ_000135"
     CDS             62708..63028
                     /locus_tag="ACG8KQ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103113.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            63169..63360
                     /locus_tag="ACG8KQ_000136"
     CDS             63169..63360
                     /locus_tag="ACG8KQ_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103114.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(63431..63688)
                     /locus_tag="ACG8KQ_000137"
     CDS             complement(63431..63688)
                     /locus_tag="ACG8KQ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9103115.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            63846..64109
                     /locus_tag="ACG8KQ_000138"
     CDS             63846..64109
                     /locus_tag="ACG8KQ_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103116.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            64133..64321
                     /locus_tag="ACG8KQ_000139"
     CDS             64133..64321
                     /locus_tag="ACG8KQ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103117.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(64404..64589)
                     /locus_tag="ACG8KQ_000140"
     CDS             complement(64404..64589)
                     /locus_tag="ACG8KQ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103118.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            64804..65175
                     /locus_tag="ACG8KQ_000141"
     CDS             64804..65175
                     /locus_tag="ACG8KQ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9103119.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(65325..65397)
                     /locus_tag="ACG8KQ_000142"
     tRNA            complement(65325..65397)
                     /locus_tag="ACG8KQ_000142"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65362..65364),aa:Gln,seq:ctg)
     gene            complement(65443..65871)
                     /locus_tag="ACG8KQ_000143"
     CDS             complement(65443..65871)
                     /locus_tag="ACG8KQ_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103120.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(65931..66689)
                     /locus_tag="ACG8KQ_000144"
     CDS             complement(65931..66689)
                     /locus_tag="ACG8KQ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9103121.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            66984..67181
                     /locus_tag="ACG8KQ_000145"
     CDS             66984..67181
                     /locus_tag="ACG8KQ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103122.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            67287..67769
                     /locus_tag="ACG8KQ_000146"
     CDS             67287..67769
                     /locus_tag="ACG8KQ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103123.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(67827..68423)
                     /locus_tag="ACG8KQ_000147"
     CDS             complement(67827..68423)
                     /locus_tag="ACG8KQ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9103124.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT        22604 a        11732 c        14579 g        19776 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 ataggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag tagatcaggt taatatcaga aattgaattt
    61741 gattctgcaa tggcattgat caaggagtta tcaccaacgt atttttgaga aatcgttgat
    61801 agagtatcac ctgattgaac ggtataagct tcgtccgcat gagcagtcgt tcccatgaag
    61861 aatgcagcgc cggcagccaa agttgttcca aaaagtaaag ttttaagtga tgtcatgaaa
    61921 aatctccttt attcgttaaa aagttttatt tgttgtgttc aacgaggaat actttaacac
    61981 gtgaaaatat ttcataggta aaagaagcat gacaatttat gacaaatcca cgttttttgt
    62041 aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa tgcagaaata
    62101 atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt gaaagaaatt
    62161 gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt gcttgagatg
    62221 ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa catcatggta
    62281 gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga cttagcaaag
    62341 gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct gacccatagt
    62401 gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt agccatgctg
    62461 aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat agttgaaact
    62521 ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat tgatttgatc
    62581 gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc gaccgcttct
    62641 tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga taagggagga
    62701 atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag aaattttagc
    62761 aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag taaagtggaa
    62821 aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata aaggattccc
    62881 tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat ttatggaatt
    62941 atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata aattaacaga
    63001 agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt tagaaaactg
    63061 attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga ataatgcgta
    63121 taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat ggaagcagga
    63181 aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga agcgaaaagc
    63241 ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca agaatttata
    63301 gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat aacagaatag
    63361 aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc tttttaaaca
    63421 tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata agaaaaatta
    63481 ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc caatgcctaa
    63541 aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa actgaaaact
    63601 tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat
    63661 aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata
    63721 caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct
    63781 gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga
    63841 ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga
    63901 tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta
    63961 ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc
    64021 cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag
    64081 catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc
    64141 atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag
    64201 agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt
    64261 gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta
    64321 gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga
    64381 ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt tttgacactg
    64441 tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc taattgttct
    64501 ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg
    64561 actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt
    64621 ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg
    64681 atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac
    64741 ttgggacgga tggaatttct ttaactgacc catatactga atatataaca aggaggtaga
    64801 aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt
    64861 ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta
    64921 aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat
    64981 gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg
    65041 gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa
    65101 ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta
    65161 gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat tattcataat
    65221 ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc tgcaaaaaga
    65281 ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga tggcagggtt
    65341 cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta catcccattt
    65401 attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt cttgatcgga
    65461 tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc cagcaggaag
    65521 ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt tgacaggttc
    65581 attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag tatactctgt
    65641 tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat ctgcgtaata
    65701 aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag gtctgcgacg
    65761 ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt cacggacaaa
    65821 aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca ttatctacgc
    65881 tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta ctagcttttc
    65941 tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt
    66001 actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt gtcatatcca
    66061 gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt tgtggtccaa
    66121 gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt ccagacagga
    66181 tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt tggaaaagtg
    66241 tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac atagacgacc
    66301 atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc gccatgagtc
    66361 ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt tttagttgtt
    66421 tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc attttctaaa
    66481 gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc tttctctttt
    66541 gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa
    66601 tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc ccctagaaac
    66661 aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct gtttatcttt
    66721 cctattataa atgtattttt attggaaatg gaagtattat attttaatat gtaggaaata
    66781 attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa tacaacagct
    66841 gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga tttttaactg
    66901 taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt ggagaaaagt
    66961 tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga gaacaatatg
    67021 catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca aaaatgacaa
    67081 actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta ttagatgaaa
    67141 aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa ttggacgtgc
    67201 ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc ctattttctg
    67261 gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta ttgtttttat
    67321 tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt tatcggactg
    67381 tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc atttggtata
    67441 cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca gcaatgattt
    67501 ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt ttgaatagct
    67561 ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat cagccaaaag
    67621 gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg atgctgaaat
    67681 tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac gatgaggcag
    67741 agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat gtttgtcaca
    67801 acctctgttt tttatttcaa ataaaattat ccaaatacgc ggaagcgatc ggcatcgttc
    67861 atgtattctt tttcctcagc cggtgtttct ggtgaaccga atactaattg cgaacgtagt
    67921 ttccagtttg aagggatatt ccattctttt gcaactgctt cgtcaataac tgggttgtaa
    67981 tgttgcaagt ttgcgcccaa tcctttatca actaatgcta cccaagtatt agctgttgcg
    68041 attccgtttg attgttctga ccaatctggg aagttgtctg catataattc aaattgttct
    68101 tgtagacctt ttaccacgtc agtatctttg aagaataata ctgtaccata accatttttg
    68161 aaaccagcta atttgttttg tgtatttggg aaagcttctg ctggagtcaa tgggcgaagt
    68221 gcttcttcag tgatttccca aagtttttca tgtgcatctc cgaaaaggat aactgcgcgt
    68281 gttgattgag cattgaaagc agttgggctt tctttaattg cttctttgat caaagttgtt
    68341 aattcttcat tagaaagtga aacattacgt cctagatgat agattgaacg gcggtttttc
    68401 aaagtgtcag taaaagttgt cataataaaa tttctccttt gatttgcgtt ttgcattttt
    68461 ttaattgatt acgaaatgaa gtatagcacc ttacaaaaag taagtaaaca gatatgctca
    68521 caaaaaataa gcgaagttca aaaaacaatt ttaataagaa aaatcgttat gtaaggattt
    68581 tcttagttat cgctttcatt gttgtgtttt aggaattttt gcttatttga aaaaaacagc
    68641 tgatatataa ttattaaatc tctatcttct tttttttatg atattgccat t
//
LOCUS       DAZKCE010000003        54925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000003 DAZKCE010000000
VERSION     DAZKCE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.003"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..798
                     /locus_tag="ACG8KQ_000148"
     CDS             151..798
                     /locus_tag="ACG8KQ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9103125.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            813..2339
                     /gene="zwf"
                     /locus_tag="ACG8KQ_000149"
     CDS             813..2339
                     /gene="zwf"
                     /locus_tag="ACG8KQ_000149"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9103126.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="ACG8KQ_000150"
     CDS             complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="ACG8KQ_000150"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9103127.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5448..6155)
                     /locus_tag="ACG8KQ_000151"
     CDS             complement(5448..6155)
                     /locus_tag="ACG8KQ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9103128.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6176..6958)
                     /locus_tag="ACG8KQ_000152"
     CDS             complement(6176..6958)
                     /locus_tag="ACG8KQ_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9103129.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7007..7228)
                     /locus_tag="ACG8KQ_000153"
     CDS             complement(7007..7228)
                     /locus_tag="ACG8KQ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9103130.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7309..7908)
                     /locus_tag="ACG8KQ_000154"
     CDS             complement(7309..7908)
                     /locus_tag="ACG8KQ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9103131.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7922..8599)
                     /locus_tag="ACG8KQ_000155"
     CDS             complement(7922..8599)
                     /locus_tag="ACG8KQ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9103132.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="ACG8KQ_000156"
     CDS             complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="ACG8KQ_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9103133.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="ACG8KQ_000157"
     CDS             complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="ACG8KQ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9103134.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11361..12581)
                     /locus_tag="ACG8KQ_000158"
     CDS             complement(11361..12581)
                     /locus_tag="ACG8KQ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9103135.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12596..13684)
                     /gene="murG"
                     /locus_tag="ACG8KQ_000159"
     CDS             complement(12596..13684)
                     /gene="murG"
                     /locus_tag="ACG8KQ_000159"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9103136.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13710..15071)
                     /gene="murD"
                     /locus_tag="ACG8KQ_000160"
     CDS             complement(13710..15071)
                     /gene="murD"
                     /locus_tag="ACG8KQ_000160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9103137.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15085..16047)
                     /gene="mraY"
                     /locus_tag="ACG8KQ_000161"
     CDS             complement(15085..16047)
                     /gene="mraY"
                     /locus_tag="ACG8KQ_000161"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9103138.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16073..18265)
                     /locus_tag="ACG8KQ_000162"
     CDS             complement(16073..18265)
                     /locus_tag="ACG8KQ_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9103139.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="ACG8KQ_000163"
     CDS             complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="ACG8KQ_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9103140.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="ACG8KQ_000164"
     CDS             complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="ACG8KQ_000164"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9103141.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="ACG8KQ_000165"
     CDS             complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="ACG8KQ_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9103142.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20253..20621)
                     /locus_tag="ACG8KQ_000166"
     CDS             complement(20253..20621)
                     /locus_tag="ACG8KQ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9103143.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20792..21748
                     /locus_tag="ACG8KQ_000167"
     CDS             20792..21748
                     /locus_tag="ACG8KQ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9103144.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21821..21937
                     /locus_tag="ACG8KQ_000168"
     CDS             21821..21937
                     /locus_tag="ACG8KQ_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9103145.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21992..23674)
                     /gene="recN"
                     /locus_tag="ACG8KQ_000169"
     CDS             complement(21992..23674)
                     /gene="recN"
                     /locus_tag="ACG8KQ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9103146.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23692..24141)
                     /locus_tag="ACG8KQ_000170"
     CDS             complement(23692..24141)
                     /locus_tag="ACG8KQ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9103147.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24248..25066)
                     /locus_tag="ACG8KQ_000171"
     CDS             complement(24248..25066)
                     /locus_tag="ACG8KQ_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9103148.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25075..25965)
                     /locus_tag="ACG8KQ_000172"
     CDS             complement(25075..25965)
                     /locus_tag="ACG8KQ_000172"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9103149.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25967..26191)
                     /locus_tag="ACG8KQ_000173"
     CDS             complement(25967..26191)
                     /locus_tag="ACG8KQ_000173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9103150.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26196..27533)
                     /gene="xseA"
                     /locus_tag="ACG8KQ_000174"
     CDS             complement(26196..27533)
                     /gene="xseA"
                     /locus_tag="ACG8KQ_000174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9103151.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27534..28388)
                     /locus_tag="ACG8KQ_000175"
     CDS             complement(27534..28388)
                     /locus_tag="ACG8KQ_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9103152.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="ACG8KQ_000176"
     CDS             complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="ACG8KQ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9103153.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28927..29352)
                     /locus_tag="ACG8KQ_000177"
     CDS             complement(28927..29352)
                     /locus_tag="ACG8KQ_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9103154.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29598..30662)
                     /locus_tag="ACG8KQ_000178"
     CDS             complement(29598..30662)
                     /locus_tag="ACG8KQ_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9103155.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30834..31079)
                     /locus_tag="ACG8KQ_000179"
     CDS             complement(30834..31079)
                     /locus_tag="ACG8KQ_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9103156.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31218..31592)
                     /locus_tag="ACG8KQ_000180"
     CDS             complement(31218..31592)
                     /locus_tag="ACG8KQ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9103157.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31675..32589)
                     /locus_tag="ACG8KQ_000181"
     CDS             complement(31675..32589)
                     /locus_tag="ACG8KQ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9103158.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32610..33413)
                     /locus_tag="ACG8KQ_000182"
     CDS             complement(32610..33413)
                     /locus_tag="ACG8KQ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9103159.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33446..34435)
                     /locus_tag="ACG8KQ_000183"
     CDS             complement(33446..34435)
                     /locus_tag="ACG8KQ_000183"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9103160.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34538..35029)
                     /locus_tag="ACG8KQ_000184"
     CDS             complement(34538..35029)
                     /locus_tag="ACG8KQ_000184"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9103161.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35221..38028)
                     /locus_tag="ACG8KQ_000185"
     CDS             complement(35221..38028)
                     /locus_tag="ACG8KQ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9103162.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38462..38755)
                     /gene="rpmA"
                     /locus_tag="ACG8KQ_000186"
     CDS             complement(38462..38755)
                     /gene="rpmA"
                     /locus_tag="ACG8KQ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9103163.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38768..39112)
                     /locus_tag="ACG8KQ_000187"
     CDS             complement(38768..39112)
                     /locus_tag="ACG8KQ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9103164.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39139..39447)
                     /gene="rplU"
                     /locus_tag="ACG8KQ_000188"
     CDS             complement(39139..39447)
                     /gene="rplU"
                     /locus_tag="ACG8KQ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9103165.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39467..39543)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39729..40370
                     /locus_tag="ACG8KQ_000189"
     CDS             39729..40370
                     /locus_tag="ACG8KQ_000189"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9103166.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40432..42063)
                     /locus_tag="ACG8KQ_000190"
     CDS             complement(40432..42063)
                     /locus_tag="ACG8KQ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9103167.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42186..42545)
                     /locus_tag="ACG8KQ_000191"
     CDS             complement(42186..42545)
                     /locus_tag="ACG8KQ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9103168.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42704..42777)
                     /locus_tag="ACG8KQ_000192"
     tRNA            complement(42704..42777)
                     /locus_tag="ACG8KQ_000192"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42741..42743),aa:Arg,seq:tct)
     gene            complement(43086..43769)
                     /locus_tag="ACG8KQ_000193"
     CDS             complement(43086..43769)
                     /locus_tag="ACG8KQ_000193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9103169.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(43897..44415)
                     /locus_tag="ACG8KQ_000194"
     CDS             complement(43897..44415)
                     /locus_tag="ACG8KQ_000194"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9103170.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            44626..46470
                     /locus_tag="ACG8KQ_000195"
     CDS             44626..46470
                     /locus_tag="ACG8KQ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103171.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKFYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            complement(46515..47519)
                     /locus_tag="ACG8KQ_000196"
     CDS             complement(46515..47519)
                     /locus_tag="ACG8KQ_000196"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9103172.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(47530..48270)
                     /locus_tag="ACG8KQ_000197"
     CDS             complement(47530..48270)
                     /locus_tag="ACG8KQ_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9103173.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            complement(48340..49143)
                     /locus_tag="ACG8KQ_000198"
     CDS             complement(48340..49143)
                     /locus_tag="ACG8KQ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9103174.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            complement(49143..50225)
                     /locus_tag="ACG8KQ_000199"
     CDS             complement(49143..50225)
                     /locus_tag="ACG8KQ_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9103175.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            complement(50366..51634)
                     /gene="dltD"
                     /locus_tag="ACG8KQ_000200"
     CDS             complement(50366..51634)
                     /gene="dltD"
                     /locus_tag="ACG8KQ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9103176.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(51637..51870)
                     /gene="dltC"
                     /locus_tag="ACG8KQ_000201"
     CDS             complement(51637..51870)
                     /gene="dltC"
                     /locus_tag="ACG8KQ_000201"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9103177.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            complement(51915..53120)
                     /gene="dltB"
                     /locus_tag="ACG8KQ_000202"
     CDS             complement(51915..53120)
                     /gene="dltB"
                     /locus_tag="ACG8KQ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9103178.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(53117..54631)
                     /gene="dltA"
                     /locus_tag="ACG8KQ_000203"
     CDS             complement(53117..54631)
                     /gene="dltA"
                     /locus_tag="ACG8KQ_000203"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9103179.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(54642..54791)
                     /locus_tag="ACG8KQ_000204"
     CDS             complement(54642..54791)
                     /locus_tag="ACG8KQ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9103180.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT        15860 a        11742 c         9227 g        18096 t
ORIGIN      
        1 acgaaagaat gagtcttctc tatcatcttt cgttttttgt tgtaaaaacg tctaaaaatc
       61 ctttgttttt tatttagaaa aatagtcaat taacgttttt tttgttacac ttactaatga
      121 tggtttttta ccatgaaagg atgatcgaat atgacaccaa atcgagaaga ttatttgaaa
      181 atcattttag aactaggtgg agatacaacg aaagtcaaca ataaacaaat cgtatctagt
      241 ctagatgtct cagctgcatc tgtcagtgaa atgatttcta aacttgtgaa agaagagctt
      301 gtagaacatt ccccttatca aggtgtacaa ttgactgcaa gcggactgca aaaagctagc
      361 agtctgattc gcaaacatcg cttatgggaa gtttttttag ttgaacattt agactatagc
      421 tggaatgaag tccatgatga tgctgaagta ttggaacatg tgacttcgga acatcttgca
      481 gatcatttag aaaactattt gaatcatcct gaacattgtc ctcatggagg atcgattcct
      541 aaaaaagagg aagttgtcca tgaagaacgc cgacaaacat tggagtccta tcctgtcggt
      601 acaaaagtgc gtatcgcacg agtgcttgat gaaaaagaat tgttggatta cttagtcact
      661 attgacctga atatcaacga agaatatgag atcgttgaca tcgcagctta tgaaggaccg
      721 atcactatcc aaaatgatag aaagaaaatc cccgtcagct ttaaggcggc cagcacgatt
      781 tttgtcgata aaatataaag gagtgtatcc acatgtctaa tgaaaaaaat gtattgttta
      841 cgatattcgg cggtacaggt gacctagccc agcgtaaatt gtacccgtct ttgttccgcc
      901 tttacagaaa aggaaattta ggcgagcatt ttgctgtaat cggaaccgct cgccgtccct
      961 ggagcgatga gcactaccgt gaagttgtca aagaaacaat ccgtgcattg aatccttcag
     1021 atgaagaagc aactaccttt gcaagccatt tttactatca atcccatgat gtaaatgatt
     1081 caacgcatta tcagacatta aaagaattgt ctgaccgatt agatggacac tatcaattaa
     1141 acggtaaccg tgtctattat ttggctatgg cacctcaatt ttttggaacg atcgtttctc
     1201 atttgaaatc acaaaatatt atgacaaaaa atggcttcaa tcgtttgatc atagaaaaac
     1261 cgttcggttc tgattaccag tctgcttttg aattgaacca gcaaatcaga gaagtattcc
     1321 cagaacaaga tattttcagg attgatcatt atttaggaaa agaaatgatc caaaacatct
     1381 cagctatccg ttttgccaat aatatttttg aggctcaatg gaacaatcgc tatatcgaca
     1441 atatccaaat tacttttggc gaaaacctcg gtgtagaaga tcgcgggggc tactacgatc
     1501 atagtggcgc acttaaagac atggtgcaaa atcatatttt acaggttgta gcattacttg
     1561 caatggaacc gccagtagca ttttccgaaa aagaaatccg tacagaaaag atcaaagcat
     1621 tgaaagctat ccgcatttat aacgaagaag aagtgttaga aaacttcgtt cgcgggcaat
     1681 atgctcaagg tgaattggat ggtgtacaat tcaagggcta tcgcgaagaa gacaaagtgg
     1741 atgctcaatc agagactgaa acattcgtcg caggcaaatt cactatcgat aacttccgtt
     1801 ggtcaggtgt tcctttctat gtacgtacag gcaaacgttt gactgaaaaa gggactcgga
     1861 tcaacatcgt attcaaacaa gttcctgtta atgtattcaa aacatctgtc gatgaaccat
     1921 gtgacgatac gacacttcca cctaatgttc taacgatcta tattcagcca actgaaggat
     1981 tttctttatc cttgaatggg aaagaagtag ggcaaggatt tgaaacggaa ccaatcaaat
     2041 tggaatttag aaatagtgca gaaatggttg aaaatagccc agaagcttac gaaaaattac
     2101 tattagatgc gttgaatggt gatggaacga acttctctca ttgggaagaa gtcgctcaat
     2161 cttggcatat cgtggatgtc attcgaaaag cttgggacca aacacaaccg gattttccca
     2221 actacaaagc tggatcaatg ggacctaaag cagctttcga actacttgaa aaagacggat
     2281 tcgaatggat ctggcaacca gataactggt atcgtgaacg tggaaaatta aacgattgat
     2341 aacaaaaggg ggtgtgacaa agaacatgtc acaccccctt ttccctttta cttttcttcg
     2401 aatccttcag ctacagcttc aggatagaag tcttcaacga tttttgcaca acgaccgcat
     2461 agatgaggaa gtttttcatc tactcctaca tctttacgaa cttggcgaca gcgatcgcaa
     2521 gtttccccgt ctgctttttc cactgtaata gcaacatcat caaataccat agcgttatct
     2581 ggcacttgtt catctgctgc cataacttca aagtagtctg gtgaaacgat caataattgt
     2641 gcaatatctg catccaaagc ttttaacatt tcacgaactt gttgttttgg ataaacagtt
     2701 agttttgctt ccaaagattt accaatcaat ttgctgtttc ttgcttcttc caatgctttt
     2761 aagacattat cacggaagtc catgaaagca gtccaggtat ccatcaattc ttcttggttt
     2821 gcaaactctt cataaccagg aaactcgctt aactgaacgt actcctcttc ttctttcagg
     2881 aatgaccaga tttcttctgc tgtatgcgga atgataggag tcaataattt tgtcaaagct
     2941 acagctgttt gatagaaaac agtttgcatg caacgacgtt gataatcatc ttctgcttca
     3001 atataaacta catctttagc aaaatccagg tagaatgacg atagatcaac cgtcaagaag
     3061 ttcataactg tacggtaaat atgcatgaag ttatatttgt cgtacccttc ttctcggatt
     3121 tctttgatta cttggttcaa tcgaacggtc atatatttgt ctacagaacg taaatcattg
     3181 tacgaaacag tatctttttt aggatcgaaa tcttctgtat tcgctaataa gaaacgcatc
     3241 gtatttctga ttttacgata tacttctgcc acttgactta agatatccat agaaacacga
     3301 acatctgctt cataatctac actagatacc cataagcgaa ggatatctgc accaaattgt
     3361 ttgatcactt tttctggtgc aatgatgttt cctaaagatt tactcatctt acggccttca
     3421 ccatctaaag caaatccttg agaaataact gatttgtatg gtgctacacc attgatagcc
     3481 acactagtcg tgatacttga gttgaaccag ccacgatatt ggtctgatcc ttctaaatac
     3541 atatctgctg ggaatgataa ttctggtcgc tgacgtaaaa cagcttcatg agaagaacca
     3601 gaatcaaacc agacatccat gatatctttt tcttttgtaa attcgccatt aggagaacct
     3661 gggtgggtaa atccttcagg aagaagttct ttcgcttctt tttcaaacca gataattgat
     3721 ccatgttcag caaataattt tgccacatgt tcgattgttt ctggtgtgat gattgcttct
     3781 ccattttcag catagaaaat cggaagtggt actccccatg cacgctgacg ggaaattacc
     3841 caatcgccgc ggtcacggat catattatac aaacgtgttt taccccatgg gataacccac
     3901 tctacttttt ctacttcatc caaaatattc tgacggaatt tatcgataga tgcaaaccat
     3961 tgtggagttg cccgatagat cacaggcttt ttcgtccgcc aatcgtgagg ataactatga
     4021 gtaaagaaat ctagcttcag caaagctcct ttttctttta atagatcagt gatcatagga
     4081 tttgctttgt cataaaagat accttcaaat ccaggtgctt cttctgtaaa caatcctttt
     4141 gagtctacag gtgacactac agatagttta tatttgctac cagcgatata gtcatcttcc
     4201 ccatgtcctg gagcagtatg gactaatcca gtaccagcgt ctaaagtaac atgatcgcct
     4261 aaaatcacaa gtgattcacg ttcatagaat ggatgacttg cagtcatata ttccatttct
     4321 tgtccagcaa attcttttag aactgttaca ttttcccaac cgatttctga ttggactgtt
     4381 tctagcaaat ctttcgctac gacaaactta cggccatcgg cttccaccaa tacatacgta
     4441 aattttggat ttacagcaat accttggttt gctggcaatg tccatggtgt tgtcgtccaa
     4501 atgacaaaag ctgtatcttc atcaagaata tctttgccgt ctttgacatt gaaagctaca
     4561 aaaatagatg gagattttac atctttatat tcaatttcag cttccgctaa agaagactca
     4621 cttgacggag accagtaaat tggtttcaat cctttgtaaa tatagccttt ttcagccatt
     4681 ttaccaaata cgcgtacttg tgcagcttca taagaaggat ctaatgtaac ataaggatgt
     4741 tcccaatctc ctgcaactcc taggcgtttg aaatctgctc gttgcttatc tacttgggaa
     4801 agtgcatatt cacgacattt ttccaagtat tctgccatgg acatttcttt acgtttcacg
     4861 cctttattag ccaatacttg ttcaatcggc aatccatgtg tatcccatcc tggaacatat
     4921 ggtgcacgat agccagacat tgattttgaa cgaatgataa tgtctttact gattttattc
     4981 aatgaatgtc ctaaatggat attaccattg gcatatggag gtccatcatg aagaacaaag
     5041 ctaggttttc cttcgttcaa ctcttgtctt ttttggtaga ttcgattttc ttcccattct
     5101 ttttgccact cgccttctcg gtttggcaaa ttaccacgca tagggaattt tgtttttcct
     5161 aattgcaagg tttctttcat tttcattcaa ccatacactc ctttatttgt tttccggaca
     5221 caaaaaagca tgttcagccg caagggacga acatgccgtg gtaccaccca aatttagttg
     5281 catctatcta gtgcaaactc tctgtgacca ataacgaggt cagccgctgc ttcttactct
     5341 aatttcggaa aagcaggtct caagtagagg atctttcacc atgcagtgtc ttaccgaact
     5401 tccactctca tcggctcgct ttaagaataa caaagtgctt ttctgttcta ctcgttttca
     5461 ttttcaccag gcacgatcga gatatcaata gcctgtgtat caaatgccgt cataggtgct
     5521 tctatgactc ctgtgtctga gtttacatca gtatcatttt cattgtcaag ctgttttgct
     5581 aaaacttctt tgatcacttc atggctgtca gtcacgtagc tggagaatgg ttgtaaaatt
     5641 tcatcccatt ccggactttt cacttgttct aattgagact caagcatcaa gctgatacgt
     5701 tgatggaata ctcgtgtttt tttcttaaga tcttcagttt ctgtcgcaag ctgtcttgct
     5761 ttttctgttg catctgtcaa gatttttctt gctttttctt ctgcatcagt tgttaattga
     5821 tgtgcacgtt tttcagcgtt cgcaactaac tcatctgctt tgttttgtgc agaagtaacg
     5881 atcacttcgg attccttgct tgcacttgtt tttactttat cagccgtatc ttgagctacg
     5941 atgattgatt ggttcaaagc atcttttaat tcattgaagt actcaagttt ttcttcagaa
     6001 tgtttgactg ctttttctaa ttctcgattt ctttgagtca attcctcata gtcacgaaca
     6061 actaaatcta agaaatcatc tacttcgtct tgattatatc cccgcatttt tgtcgggaat
     6121 gtcttatttt ggatatctaa tggagttaaa gccatgagta aaccaccttt ttattttatt
     6181 tacgtaatac accgaacagt accctgtact tttctttttt cgtcttcccc tcaagttctt
     6241 ggatctggac tctgccataa cctctgactg aaataatatc taatagttca agtacaaaat
     6301 caggtcttgt attttctgtc cagttcactt ttactttacc agcttcgatc agctgcttgg
     6361 accgctgtct ggaaatatta taaatctcag agatcacact gtctagtcgc atggaactaa
     6421 ctgttcctct ttcttgtgtc cagctatctt tcggcataat catttctgtg tatttgattg
     6481 gttccaagcg tacagcaacc tttccaatct ttgttacttg tgcagcaaca aaatgatcca
     6541 cctcatttgc aataaatact tgccagcgat caccgtccga gatgatatcg ccaaagtaag
     6601 agcgtttgat ccccgttccc actaaagttc caaggatttt cccatgactt aggctggcaa
     6661 atttgctagg ataatggatt tcatacaatg ctgtttcaaa atcttcctct acaggttcgt
     6721 aataatcggg gaaaatcaaa catctttttc tttctgcctg ttcatatccg ccataaaaca
     6781 tgaaacgcaa atcactgctt tgtcgaatca gtgtttctaa aatataggat tgtcttgggt
     6841 caagaaattc ggtcagatag ggtgcatatt gtccttgaac ctgttcaatc caatcgccta
     6901 ccgaatcgat aaatgtacgt tcatctgccc gaaagtgctg gtatacattt gcatccaaaa
     6961 ttttcccctc ctatctattc tgagaataaa gattttgttg cttcaatcaa taaactaaat
     7021 ggacagttat ccatataacg atctgtgcta aaccttgtac ggctaagttc aagatcagga
     7081 ttgcccctaa aattgtgaaa tccaagggtc caatcctcaa aggcagacga tcgaacaaac
     7141 tgatatacgg ttcgcagatc ttagctaata atcgtcctag ccctgattga tacccgcctg
     7201 gaaaccaaga gagcaatgca tataccatca agataatgct atagaaatag gctgcccgtt
     7261 gaagtaaatg aagtaatagt gcaatagtat aaatcgtagt tcctcctctt acagatcaaa
     7321 caaattcgca tctgcaaaag attgagcggt tgaactgtca atctccatcc cagtcggtgt
     7381 acaaagaaaa atctcatctc caacacgttt gatatctcca tctagtgcat aaactgttcc
     7441 tgttagaaaa tcaacgattc ttctcgcttg tttttcttct actaaatgaa aattcactaa
     7501 ggttgcttct ccagcaatga ttcgtttagc aatcgccata gcttcagaat aagctcttgg
     7561 ttccattaca gtgatttttc cagcaagatt tgctttaggt tgtgtagagg ttcttgaagc
     7621 tgttctttct ttcgctacag tttttgtagg tgattgatgt agttcaatta cttttttctt
     7681 ttcagccggc tgaatacttt catgcgttac aggacgtgaa gtcgccgttt tttgtttttc
     7741 atgagtattc gcagcagata cagaatgagt tgattgattc tgtgttgtat tttttttagt
     7801 tggctgtaca gccttttttg ttgtttcagg ataatcttgg tattcgtact cttcctcatc
     7861 agctaatccg aaaaaattag caattgctcc tctgtttaaa agcggcattt ttgttcctcc
     7921 tttattcgtc tttgaatagc gctgttccta cacggataaa tgttgcacct tcttcaatcg
     7981 caatttgaaa gtcttgactc atccccatac ttaattcact acatggtgca tgggcataac
     8041 cttttgcttg aacaagatca cgcaattctt tcagcgtacc aaacacttta tggatttgct
     8101 catcagaagc ttctttaggc gccatagtca tcaacccaac aaccttgatt tttgtgcagg
     8161 cagctaaaga ttccacaaac ggtaaaagct catctggtga aattccatgt tttgattcct
     8221 caccagaaac atttacttca ataaaacagg ataattcttt ctctaaccgc ttctggattt
     8281 cttctgctaa tcttaaactg tccaacgcat gaaagtaatc aatttcattt acaatcaatt
     8341 ttacctttct gcgctgtaaa ttaccaatta aatgccactt tatttcagga aaatcaaata
     8401 aatcttgttt cttttctaat aatttatcta ctcggttttc tgcacaattc ataacgccag
     8461 ctctaaccaa ttcttcagct tgagcggatt caacagattt tgttacagct actaatgtaa
     8521 cttctttcgg atcacgatga acaagagcac acgaatcctg aatttcttgc ttgattttct
     8581 gcaagttgtc agcaatcatg tgctcttcct ctcctttatt ttttacgacg gaaaaacggt
     8641 ggcgtattta attcatcatc gccatgagat tttgtttctt cgcggttgaa tgtatcaaat
     8701 tctttttttt cgatattgtc aaaattggaa tcatcaactc taggacgaac attttgttca
     8761 cgtctgatat cccaatcgcc aaaactgttt tcttcttctg aagaagttgg tttagcttga
     8821 tccatatcta atacagtctt ttgagcaggt gattgcattt gtgcttgtct agctggtcgg
     8881 ctagacttgc gttccttttt tgattcatcg atacctgttg cgatgaccgt cacacggatt
     8941 tcatcgccca tttcttcatt gatagatgta cctagaatga tatttacatc gccagttgct
     9001 gcatttgcta cgatatctga agcatcttgt gcttcgaaca atgtcatatc caaaccgcct
     9061 gtgatgttaa gaagtacttg ttcagctcca tcaatagatg tttcaagcaa tggagaggag
     9121 attgcttttt tcgttgcttc tactacgcga tcttctccac tagccacacc gatccccatc
     9181 aatgctgtac cttggttttc catcactgtt ttcacgtcag caaagtcaag gttaacgtaa
     9241 cctggcgctg tgatcaaatc agagatacct tgtacacctt gtcgtaaaac attatctgct
     9301 tcacggaaag cttcaagcat cggtgtcttt ttatctacaa cttctaataa acggttattt
     9361 gagataatta ataatgtatc cacattttct ttcaaacgag cgataccttc tgcagcaaaa
     9421 cggccacgtt tcggtccttc aaaagtgaat ggacgtgtca cgacaccaac agtcaatgcg
     9481 cccaattctt tcgcgatacc tgcaacgatt ggtgcagcac ctgtaccggt tcctccaccc
     9541 attcctgcag tgatgaaaat catatctgca ccgtctaaag cttcgcgtaa ggattgttca
     9601 ctttcttctg ccgctttttg tccaacttct ggctgagaac cagcacctaa accacgagta
     9661 tatttggggc ctaattggat aactgtttca gcttttgaat tttttaatgc ttgcacgtct
     9721 gtgttggctg taatgaattc aacaccttta acgttttctt caatcattcg gtttacagca
     9781 tttccaccgc caccaccgac tccgatgact ttgattactg cgccgtcatt gatattatta
     9841 tcaattgaga attccatatc ttattcctcc caggatgaat taatcaaaaa tatttgtaaa
     9901 gaaacccttg attttatttt ttacattttc tccggactct tttggttccg gctcatcgta
     9961 gattgtttct tcaggtgctt catagctctc atatgtgttt gttgtttctt gttcaactac
    10021 catttgacga gctgctgagg tttcacccgt aacagcaatt tttgccaatt gatacacttc
    10081 actcaagttt gctgaataat ccacgatact tattacattt gtaaataccg ggtttctcag
    10141 ccccatgtga tttggaacat acaattttac atttacgccg aagatttctt gtgccaagtc
    10201 cacaacacca ggaagactag cagctccgcc tgtcaatacg acgccacctg gcaactctaa
    10261 tgcttcgatt tggtccaatg catcttttgc tttgttgaaa atctgttcca ttcgagctga
    10321 aataatttct gacaagtagc gttcatcgac tttcactggt tctgactgac caatcacatc
    10381 tactggaaat tcttcatctg gtgatgtccg ttctgggtat gcatccccgt agtttatttt
    10441 caacgcttct gcattgttaa atgacgtatt taatacaata gagatatctt tagtgacaaa
    10501 ttcgccacct tcttggtcaa gattggtaaa cttcaattgc ttatcatgca taacagccgt
    10561 tgttgtttgg ccgccaccca tatcaatgac gatcgtacca aaatcttttt ctccgtcaga
    10621 aagaattgat tcagtcagcg ctaaaggagt aatcaccatt tcgttgacga ttaatccggc
    10681 attttccacg catttacgta tgttatggat gatcgtttta ggtcctgtaa acaacagacc
    10741 gtacatttct aatcttacgc cgatcattcc acgaggatct ttgattccct cgaacccatc
    10801 gacagtaaaa tcttgtggca atattgacac gatttgacgt tcaggaggta ttgaacgaac
    10861 aagggcagct gaagccacat ttctgacatc ttcatcggtg atttcctttg aatctccgtt
    10921 aactgcaatc attccctggc aattttctac ttcaagcatg tttgctggca aaccgacact
    10981 tacgcctttg atctggatac ctgctttttc ttctgcttgt cgtactgctc gttgtatcgc
    11041 ttggaccgtt ttatcgatat caacgataat gcctcggtta atcccttctg attttgcgtt
    11101 tcctactcca ataatattca tttggctgtc gatatattca gccacgacaa ctttaacaga
    11161 tgtcgttcca atatcaaggc ctacatacat tcctgttttt gccatgaatg ggattccctc
    11221 ctacttacta ttccatatac aagcgaacaa ctagcgtccg ttttaaaata tacttattaa
    11281 tagatatatt cactgcttcc aattctacca taaattactg gtttttagaa agtacattct
    11341 tactttttaa agttgtttta tcaattttca tttaaacttg acggagttgt acttggatta
    11401 tcttctattt gacttgtttc acttgaatca tttgaatcag atgatccgtt tgtgctagac
    11461 gatccaggtg tttgtgcttt ttgcgtttct gcctcttcac ttgattcgtt tgtttgatcc
    11521 gcattttctt cactttcagt tgtttcttcc gattggcccg attcttccgt ttcttgagtg
    11581 ctaccgtaag gatgcgagaa gataccaact tccatgtcga tcacaccttt ttcatccata
    11641 tctttggcta cttgcgaata atatttcatt tggttcgcaa gattattgat atttacaata
    11701 acttgattgc cgtcattcat attcaattgg atcagatttt tattgcttgc tcttggcgtg
    11761 tacttgattt cagaaattgc tttttgtaat tctgatgata actgattgta ttgctttgtt
    11821 aattctttga tttttgcaga gtctttgaac tcttccaaga tagggagatt ttttgtagga
    11881 tttgctactt tttcatctaa tactgttcca ttttcaatca ctgggtagta ttgattgttt
    11941 tttgcgatca aagcaatctc tttatattct gttacatcca aatcgaattc attcatccct
    12001 ttgaaatgta tatttgctgt ttcgattctt ggctgtgctt ttttaaggcg atcgatataa
    12061 gtagatcgat gaaaatactg tccccaaaca ttttctccta atgacaattt tgtatcagag
    12121 atgatttctt ggctgttgac tgtcttattg cctgatactg caactgcttg taatttgcta
    12181 taaggagaaa cataatagat caaaaaaatc agcggtatac ccaatacagt gatgatcaaa
    12241 gctaaccgac gaaatagtac tttgtgccgt tgacttttta aattgggcag ccggttcaaa
    12301 aaagaaccgt tatatggtcc accgtttttc ttttgcttga tctcttcaaa aaattcgatt
    12361 tttttctcgg actggctttc ttcttgctgt tcctcttgaa ccgttttttc ttcttcagaa
    12421 tctgaaaggg gtgtatctaa tgttatgtct tctttttttg gctcttggtt tttcatctct
    12481 tgttctgcca gatattttcg gttagccttt tgccaaggag tcaaattgtt ttcatcagaa
    12541 gacggatgag acaatttttt tggttctttt ttcttactca ctaagaccac cgcctttatt
    12601 ttttgattat ttgttttact aagtcataaa gacgttttga agcatcaggg attccttgtt
    12661 ctttagaagc tcggcacatt tgcttttgca attcttcatc acccatgatt tcgttgactg
    12721 tctgacttag tgattcacct gttagttcat tatctgcaat catttttgct gctcctgcgt
    12781 gaacaagact cattgcattt ttagtctgat gatcattcgt aacatatggg ctgggaacca
    12841 acactgccgg taaccccaga gctgtgaatt ctgcgatcga agttgcacca gcacgcccca
    12901 ccagcagatc actggaagcc atgacctctg ccatattttt gatataaggc tggacactaa
    12961 tattttgtat ttcaggattt attttattta ggatctcttc ataatatcta tctcctgaag
    13021 catacaaaac ttggtaagaa gaaggaatat attcacttaa aaaaactgat actgcttgat
    13081 tgattttaag tgctccttga ctaccgccaa aaatcaagac tgttttcttt tctggatcca
    13141 aatcaaatgt tttcaaaata tctgattttt tagtatctgc tacttcttgt gctcgaggat
    13201 tacctacaag aactgctttg ttttcaggaa aaaaggaagc cgcatcttta aatgaaagag
    13261 cgatccgatc cacatatcgg cttaaaaatt tattggtaac acctggtaca ctgttttgtt
    13321 catggataac tgttggaata cccattttgg ctgcagcgta cactaccgct ccggaaacat
    13381 atcctccagt accgataacg atatctggtt gaaaatcact gaggattttt tttgcttgac
    13441 ggatactctt aagaaacaat cggaccgtct tgatgttatg catggaaatt tttcgtttaa
    13501 atccttgaat ctccaaggta tggaaaggca ttcctgtatc agggacgatt ttgttttcta
    13561 gacctcgttt tgctcccaca tacatgaatt cagcattcgg ttcttgagtc tttacataat
    13621 tgacaaaggc aagagctgga taaatatgtc cccctgttcc tccgcctgta accaaaattt
    13681 tcatttttta tcgctcgctt ctactttttc tattttaatc ggttcacagc ttccatgaat
    13741 ctgtttcctc tagtttcaaa atttgggtat tgatcccagc tggcattcgc tggtgataat
    13801 aaaactacat caccatctgt actgttttct aatgctaaag gaactgcttg ttctacagaa
    13861 tcggctgtta ctattgtctc gatgcctgct ttttttccag catccatcaa cttttctttt
    13921 gtttggccaa atacgaccat tgctttgatt ccttttaatg aaggaattaa ttcatcaaat
    13981 gtatttcctc tgtctaatcc gccagccaat aacacgacct tagacgtttc aaatccactt
    14041 agagccattt tagtcgctaa aatattggtt gcttttgaat cattataaaa ttttctgcct
    14101 tggatttctc ctacatattg cgtacgatgt ggtacgccat ggaaaaaatg caacgtttct
    14161 cgtatagctt catttgatat accatacaat tttgctacag aaatagcagc tagtgcattt
    14221 tctacattat gcttacctgg tacgccaagt tcagatattt ccattatttt ttctttttta
    14281 tagtatattg agtaatcgtc agcatacaca ccgtcttcca acacttcttt tgtcgagaaa
    14341 ggcagtacgc gtgcttttgt tctttgcgct aattcttgta gttctgactg attccagtta
    14401 aggatcagat aatctttttc tgtcatgttt ttttgaaggt tccatttagc tttgacatat
    14461 tcttttcttg taccatgata atcaatatgt gcttcataga tattggtaat caccgctata
    14521 tgagggcgga aatctgtgat tcccatgagt tggaagctag acaattccat cacaatttta
    14581 tcttctgatt ttgcttcttg cgctacacca cttgctggat acccaatatt acccgctaaa
    14641 cgtgcaattc cttgatctgc tcctgcattc agtagtagtc ctgtcatcgt tgttgtcgtt
    14701 gtttttccgt tcgttcctgt aatcccaata attgggcatt ccgctacttc gtaagctagc
    14761 tcaacttccg taattaccgg aatacccatt tcttgggctt ttgctactaa aggatgactg
    14821 tagggaatcc ctggattttt gaccatcaat gaaaattctt catccaacaa ttcgattgga
    14881 tgactacctg ttattacttt tatccctaaa gaaagcagct cttgtgcttc cggattttcg
    14941 tcaaaaggtt ttccatcatt aacggtgaca agtgcgccca attcatgtaa taatttggcc
    15001 gcacttactc cgctttttgc gagtcctaag acaagcactt tcttattttc atatgttgat
    15061 atttttttca tttcccgtca tctcctacca aacgatccat aatgtgatca acgaagtcac
    15121 aatgctgatc aaccaaaaaa ctgtatcaat cttccactcc gaccatccgc acatttcaaa
    15181 gtgatgatga attggagaca ttttaaaaat tcgttttcct gtaagcttaa aggaagtaac
    15241 ttgaagcatc acacttgctg tttccataac atagatcaaa ccaatcaaaa gcaatgtcca
    15301 ttcttggttt agcattatag aaattgccgc taaaagtccg cccaacgcta atgaaccgac
    15361 atctcccata aaaatcttgg caggttttct gttgtagacg aagaaaccca gtaaacctcc
    15421 taaaacactt aagcaaatga ttaatacatc atactgatgc tgatgccaag caattagacc
    15481 gtaagctgca aaagagatac ttcctaagcc agcaactaat ccatcgatgc catctgtcag
    15541 atttactgca tttgaaaatc caacaagcca gaagagagcg aaaataccat aaatgattcc
    15601 taaaggtaga tctagtccga agaaattcaa tacacctgga taaccttcag aacggtaaac
    15661 aagataaaag atgattcccc ctagaatctg tccaatcaat ttctgcttcg agttcagtcc
    15721 catgtttctt ttcttgaata tttttataaa atcatcaaga aagccgatta ctccgtataa
    15781 cgctaataca aaaagcaaga tgaaaagagt aggagttagc tgtttttgcc aagctccaac
    15841 ccaaattcct gtcaaaatag agccgatcag aaacactaag ccgcccattg taggagttcc
    15901 ggctttgcta ttgtgccact ttggtccctc ctcacgaatc gcctgacctt gctttttcat
    15961 ttgaaaataa ccaataaata gcggcattgc ggcaattgtc attgcacaac tgctgacaat
    16021 gggaatcaat gcttgtgtcc attccatgct cttttctctc ctatttctct tcttattctt
    16081 ctaaagtaaa actaagtttg tcttcagtga ttttttcata tggagcaagt tcttgtttga
    16141 cacaataacc atctccatca aaactaactt caataccgag gattttcccc agcttcatga
    16201 tatctgcttt tgaccatcct gttacgtcag gcatcagctt atcaccacca gtatatagga
    16261 tcagtttttc tccagagatc aactgatcac cgttggcagt agattgtttt tgtacttttt
    16321 taccattacc gatcactaca ggttgcagtc cacttttttg agcatcagct gctgcaacgt
    16381 ccgcttcaag attacgataa tcggctacgg atactttttc tgtttttgtt tctgtatctg
    16441 aatcttcttc agtttcttta aaatccattg cccgtttcat caatggattt gcgattttag
    16501 ccaatgccat tcgatcatat gtcttagggt gtttcattgt tagatacaag acatagtctg
    16561 gatcttcaga aggaaccatc tccacaatcg aatacaggta ggctgtatct cctgtttgat
    16621 atccccctgt atcagaagcg atttgggctg ttccgttttt tgccgataca ttgtatccag
    16681 gtacactata tacaccataa gcactaccgt agttttcgct ttcgacaaca tctctcatat
    16741 attcacggac tttttctgtt gtttcttttg aaaaaggttg ccctagaact tctgtttgag
    16801 ttgttcgctc ttctccagtt tggggatcca ccactttgct gatatatcga ggttgaatca
    16861 ttgtaccgtt attcgcaata gaagtgaaag ctttcatcat ttggaaattc gtcacaccta
    16921 ccgcttgtcc ataagcactc attgctcggt cgacaatatt taatgtaggt aaagcaccgt
    16981 ttacttcatc atctaatcca gaatgagtac tttgtccaaa tcccagtttt tgtaaatagt
    17041 tataccatcg tccgcctaat cgctgttcca gtatgaccat cccgacgtta cttgaccatg
    17101 aaagagcttg acgcatggtc agcacacctt tttcaccaaa gtcatggtca ttgatcgtag
    17161 catcttctac ttggattttt ccagattgga aggtttcatt ctcgttgaat tctccttctt
    17221 cgatagcagc tgcagtagtg aaaactttca tggttgatcc tggttcataa ctatcttgta
    17281 ccaagaaatt acgccagata gcatcttttc cagtcaatcc ttccattgtt tcaggattga
    17341 aagtaggtcg ttgtcccatt gccaagattt ctccggtttt tgctttcatc aggactgctg
    17401 tcaattcttc tggctgatat tcttcattta cttgatccat cagtgtttct aaataacttt
    17461 gtagtctgct gtctaaagtc gtgtagatat cttgtccgtc gacagctttt tcttcttctg
    17521 ccactgtacc tggcagagga ttttgaaaat tatctttttg ataaataatc ttaccatctt
    17581 taccgctcaa gatatcattg taagcagact ccaaacctag tttaccaact aagccattct
    17641 catctttgtc tggaactgcg tatccaataa aatgagaaga aaagacgcca tttggataca
    17701 tccgagcctg atggtcgaca aaataaagac cagcgacacc ttcttttttc atgtccgctt
    17761 cgatgttttg cttggtttct tgggagatat ttttcccata gctaccaaat tcaacttgat
    17821 acagctcttt ttttgcgccg tcctctaaaa tattcagtgc atcttttttg tctatagaaa
    17881 gattcttatg caggatttct gctattttat caaaattctt ttcttcaaca tacagttttt
    17941 tatccccaga ggtatatgtt tttgagagta tcgctttaat tgaataagag gaggcatctt
    18001 ctgcgagcgc cacaccgtta cgatcataaa tcgttcctct ttttgctttt actacttcac
    18061 ttccttggta caactgtttt gttttttcag ctaatgaggt accagcaaca tgacctccaa
    18121 taacgatata cgagaaacgg acggcaaata aaaagaacaa tccaatactc gtagcaaata
    18181 aaatgatacc tacttttttg cgattgttca tcggatttag atttttcttt tccataaacc
    18241 gccgaatttt atttttaaga ctcatttact tcactttcct taaattgtcg tcattaatag
    18301 acaatccttt ttcttctgcg atttgtttaa tccgttcagt acgagaaagt tcgttttttt
    18361 cttgttctaa actcgttttt tcttgctttt tatcattgat ttctgtttgt attgacgtga
    18421 tttctttttc agctccgctg atatttgtgc gcagcataac tgttaaaaca cataaagcaa
    18481 caatagcaat cgccacgctt gccacagcaa ttttttccaa agcagaaatc tgctttaatc
    18541 gttttgccgg tgattgtgga agaatgattt cttgattcga agaagaatca ggcatttgtt
    18601 gttccggctg atccaccaat tcaggtaaat catattcgta atcttgcatt ttctttaatt
    18661 ctgccatcat tctactcctc tttcattctt attttttctg caatacgtaa ctttgcactg
    18721 cgtgatcgat tgttttctgt tagctcttcc tctgaaggaa ggattggttt acgagtaatg
    18781 actttcagtt ctggttggaa ttcgtcaggt atgacaggaa gtcccggtgg tagatcttgt
    18841 accgtactat attctttaaa catacttttg acaattctgt cttccaatga atggaatgtg
    18901 attacgctga ttcttccatt gactttcaat aaccggattg cctgttctaa agaggcctct
    18961 tcggcaccga gttcgtcatt tactgcaatc cgcacagctt gaaaaattct ttttgcagga
    19021 tgcccgcctt ttcttcttgc gggagctggg atgacttgtt taatgatttc gacaagctca
    19081 ccagttgttt ggattggttt tgtttttcgt actcgttcga tttcacgagc aatttgtttg
    19141 gaaaatttct cctcgccata gcggaagaaa atctttacta attcatggta ggaatattca
    19201 ttaattacgt gataagcgga taaaggagct gactgatcca tgcgcatatc caacggtgca
    19261 tcttgatgat aactaaatcc acgttctgct tcatctagct gaggagagga aacccctaga
    19321 tcatacaaaa tgccgtctac cttatctaca tgctcactca agcgttcttc taattctctg
    19381 aagttcgctt tgataaatgt gaccatacct tgttcgacat aagaagctaa acgcagcttg
    19441 gcgtgatcga ttgctttttg atcttggtca aacgcataca gatgcccttt agaacttagt
    19501 tggcttaata aatattcact atgtccggca ccgccgagtg tacagtctac ataaacgcca
    19561 tccggtttga tgttcaatcc atctaccgtt tctttcaaca tgactgtata gtgttgaaat
    19621 gtttcagtca ttagtcagcc tcttcctatt cttatagtcc gaaatcaatc atactttcag
    19681 caatttcgtc aaagttctct tctgctgctg cagaaaaatc ttgccatcgt gtctcatccc
    19741 aaatttcaat tcggttagat acaccgatga ctacgcaacc ttttgtcaaa gctgcataat
    19801 ttcttaacgt gctgggtata ttgattcgtc cttgtttatc aagttcgcat tcagtggcag
    19861 ccgaatagaa gaatcgcaca aaagtacgtg cgtctttttt agccaacggc atttcgttca
    19921 gtttagcttc cagattttcc cattcgctca tcggatagcc gaaaagacag ccatccaatc
    19981 cgcgtgtcag aacaaacttt tcgccaagtt cttcacgaag ttttgagggc acgattaaac
    20041 gtccttttgc atcaatatta tgttggaatt cacccatcaa cattgaaagc cccccattcc
    20101 tccactaact aaaattagtc taccacaatc ccccactttc taccaccttt tctgggaaat
    20161 ccgggaacaa ctttttttat tcataaacat ttttttaaaa atcaagctgc aaaaagcctt
    20221 ttaaaagact ttttacagct tttctatttt tttcataggt aagaaagtat gttcacaatg
    20281 atcaagacag cgtacaagac gattgtcaac agaaaaacca aacgccaagt catcttgaaa
    20341 taacgcggat aaataatttc ttcataataa tatgcttgga aaacagcaat acaaattccc
    20401 attagcaaaa cagaaataaa gaaaaacggt aagatagaaa ttgtccctgt atttctagac
    20461 aatgcatgca taccgcccca caaaaaagga atcgccaaat caggggcttt tattttaaaa
    20521 cgacgattta acgaaaataa ttttacaaga aattggcaac caaacaaaac gattaccggg
    20581 taaatatacc aaaacaagat aagtggtgtg aatgtgccca tgaacgtaaa ctcctttttt
    20641 atcgttgctt caagtatact gtatatactt tacacaactg tttcatttta tttaaattta
    20701 tgcagttata ttctatatct tacaaacaca ttccatgtat tccaattccc tatccgttat
    20761 aataaaagaa acaggcaaca ggaggaaaat catgatcaaa tattttacat tagataatga
    20821 gaaactgcgt aataccggaa aggaaactga ccaaacgatc tggtattgtg tagaacgtcc
    20881 taatcaagaa gagattcatc agctgacaca tcaatttgaa attccgtcag attacatcac
    20941 ttccatattg gacgatgcag aaaattcccg tacagaagaa ttcaatcaat caaagttgac
    21001 aaagccagcg ttattattgt tacaatatcc ttttgctaaa acaagtccta gcggttattt
    21061 tcaagtggat acatacccgc tctctattat tttgacgccc aaaaagaagc ttatcacaat
    21121 tacgaatcat gaaccactgt ttttaaagaa agtctttagc cagacctttc caaaaagtga
    21181 tatttcgcct aatctcaata tcttctttca attcatttgg caggtcacac tctcttacaa
    21241 cagttattta gctacgcttc aatctcaatg cgatactttg gaagccaaac ttcaagtatc
    21301 cactgaaaac aaacaacttt atcaaatcat ggatatccaa aaaagcctcg tttatttcag
    21361 agaagcgact tctgctaatt tagatgcatt gaaagaacta gcgcaaaata aacttttcca
    21421 agaaaatcat gctttgaaaa atcatttacg tgatgtattg gtggaaacag agcaggcaat
    21481 gactacttct cgcattcaat taaaactagt agaacaaatg aatcagactt tttcagcaat
    21541 cgtatcgaat aatctgaata atattatgaa aattttaact tctttaacga ttattttaac
    21601 tataccgaca atcgtcggca gtatctatgg aatgaacatt aagttaccta ttgctgaaag
    21661 ggatgatgcc tttatatggc tagttctatt aacattattt atttgtatcg taactaccta
    21721 ttatttaaaa aaagggaagt ttttataaaa aagccctctt gtggaaatgg aaaaaagatt
    21781 gtacacttga atcaactaat taatgacgag gttgaaaaca atgcgtgaaa aaaaacaaac
    21841 aagtactttt gctaaagtaa caaaatttgt catttggaca atgctgattt taacgattgg
    21901 atctgttgta ttgactgctg taatgagtgt catgtaaaaa taaaatatag ctatcagctg
    21961 ctgaaaagga cctgaaccac tgcgaggtgg ctcaggtcct ttttgcgagt ttcaatagtt
    22021 cttttgcatg ttcgagtgtg agttttgtaa tctcacttcc agccagcata cgtgcaattt
    22081 cttcttctct ttcttttgtt ttcaactctg aaacagaagt ttgcgtacgt ccatcaatca
    22141 ctgctttcac aatataatat tgatagtcgg cgactgcagc aacttgaggc agatgagtga
    22201 tgcacaaaac ttgagagtat ttcgatattt gagaaatttt atctgcgatg gcctgtgcaa
    22261 ctcttccgct cacacctgta tccacttcat caaaaataat acttgtcaca ccttgctcag
    22321 aagaaaaaat agttttcaaa gcaagcaaca tccgagacag ttcgccacca gaagcgactt
    22381 tcaccaaagg cttcagtggc tctcctggat tggtcgtaat gtaaaattcg atttggtcaa
    22441 atccatcatg attcaattgt ctattttctt cttttaagaa acgtacctca aactcagtgt
    22501 tttccatata cagacttttc aattcatgca gaatggattt ttccaattct tttgcgattt
    22561 ttttacgcgc ttcatgaaga tctgcggctt gttgctgaag tagaagctct ttttgctcta
    22621 gttcctcttt cattttgtcc aattgacctt ctgaaaaatc agaagaagct agctcttcgg
    22681 tgatttcgtc atagtaagcc aatattgact caatggattc gccgtattta cgtttcaact
    22741 gtcggattaa ttctaaacgt tgcgtaactt cttctaaacg actctcatct aactccaata
    22801 aatcaatctg acgactcata tcgccgattg catcttgcag caaatagtaa gcactttgga
    22861 tattttcata aattgcttca tactccacat ctagatgggc gatcccttgc agttctgaaa
    22921 cggccaagcc aacaccatct acactgtttt gttcattatt cgtcagtgct ccgtaacctg
    22981 cagctaatcc atcagcaatt ttttgataat tgctaagttt ttctctttct tcttttaatt
    23041 tctcttcttc tccgacttgc aaatcagcag cagctatttc ttcttgctgg aaatggagca
    23101 tatctattct ctgaacatag gatttttcat tttgctgcac ttttcgtacc cttttttcca
    23161 gctcacgata agagatatat tcttgctgat aactcttttt cttctgttgg aaagctacat
    23221 ctccaaaacg atccaataaa gcaagatgtg attcaggctg gagcagttcc tgatgttcgt
    23281 tttgtccttg gatatctact aaataactgc cgattcttct cagattagct aatgtgataa
    23341 tatgaccatt cactcgacaa acattttttc cagttaagga catatcccgt cgaacaatca
    23401 gattgtcctc atctgtttcg atccctaatt caaccatcag ttcagagaat ccttcttgtt
    23461 ttggtagttc aaacaaccct tcaagcacac atttttctgc tccttgacgt atataatcgc
    23521 ttgaacctct tcctcctgct aacagaccta aagcatcgat aataatggat ttacccgcac
    23581 cagtctctcc ggtcagcgcg gtcattcctt cgtgaaaaga aagccgcaat tccggaataa
    23641 tcgcaaagtt agtaatactg atttcttgta gcatcacatt cgctcctcaa cttaaaggta
    23701 atgcaggaag tctttcttca ataaaactgc ctggtcttca gttcgtgtaa tcatcaaaac
    23761 attatcgtca tcattgataa tcgaaaataa ttctttcttg taacgtttat caatcaaatt
    23821 tgctgctgcc gaagcatttc ccggcaatgt tttcaaaata acaaattttt ccatttggtc
    23881 aactgccacg aaagcgtctt ttaatagttt ttccaattta gtatttacat cttcgtttgt
    23941 ttcagctggt atgctataac ggtagccgcc atctgaagcc ggtaccttga tcagtttcag
    24001 ttcctttata tctctggaaa tcgtagcttg agtaacagat attcctttgc ttttcaggat
    24061 ttcaacaaat tcttcctgct ttcggatatc ttgctcactc aacaatcgag taatcaaacg
    24121 atgccgatct ttttttctca ttacgtcttc gctccttagc atattcttct taatcaatga
    24181 tagcttattt ccttggtata ataaagaaga atccgcatac ttctcacttg attttcacag
    24241 ttacaattta tttatggtca aactgttcgt gtgcatttga aaccacttct gccatattaa
    24301 tagctgaatt gattgttccg ctttcgggta ctttcctcaa gtgagccaag aactcgatat
    24361 ttccttctcc ccccgtgatc ggagaataac ttaattccag cacatcatat ccaccttgtg
    24421 ccgcaaatga caaaatgtct tctaaaactt tttgatgggt ctgagggtca cgaacaattc
    24481 cgtttttccc tacagcttct ctgcctgctt cgaattgggg tttgattaaa gcaatcactt
    24541 ctccctcttc ttttaaaata gcatgtaatg gaggcagcat cagttttagt gaaataaaag
    24601 aaacatcgat aacagcaact tctggaattc ctaaggtaaa gtcttcgggt tttgcataac
    24661 gaaaattcac tctttccatt acgacgactc taggatcttg tcttattttc caagctagct
    24721 gattgtagcc aacgtctaaa gcataactca tttttgcgcc attttgcaaa gctgcatcag
    24781 taaatccgcc agtggatgca ccaatatcta acaatgtttt tccttcaaca gatagatcaa
    24841 atgctttgag tgctttttct aattttaatc cccctcgaga aacataagga agttttttac
    24901 ctttgactct taattcacta ctcactggaa ttttttctcc cggtttatca aaccgctcgt
    24961 ttttttcgtt gtaaatcaaa ccagccatca ctgaacgttt ggcttgttct cttgtttcaa
    25021 ataatccttg tttgaccgct aatacatcta cgcgttcttt ttccattctt tccctcattt
    25081 ctccacatca aattgcttga tcatctggcg catcagcccg ccatcaaaag aaaaaacgtc
    25141 ctcttctaat ttgtctatga tcttgttggc ggataacagc tcaatctcca acgcctcacg
    25201 tgtcttttct aggcctaata aacgcgggta agtatttttt tccatacgtt catctttacc
    25261 agctgttttc cctaatgttt tcgttgtacc aatcacatcg agtagatcat ctcttatctg
    25321 aaaagctaaa cctaaatgtt cggctaatcg ttgaagtagc aataagattt cttcaggttg
    25381 cttcgctaat atgccaccag ccagcaaggc ataacgaatc aaatgccctg tctttctttc
    25441 atgaataaat gctaattctt ctaaagaaag ctttttcgat tcgccctcga tatctgcagc
    25501 ttgcccagca accatacctt gtgatcccgc acaaacagct aattgctgaa gcagcagaag
    25561 cttaggagaa ttacctaagt gtgccatact gattagctgg aatgctcctg taagcaaacc
    25621 atctccggcc aatatagcta atgcttcgcc gaatacttta tgattggtag gttttcctct
    25681 acgaagatca tcattgtcca tcgcaggcaa atcatcgtgg atcaaggaat acgtatgcac
    25741 catttctaaa gcagctgcta cttgatagac gggaacatca ataggctcat caaaggaagc
    25801 aacggccgtt aaaagtaata agggacgtat tcttttcccg cctgctttca ctgaataact
    25861 catcgcatca tagagtcctc tatccgttgt atgctcatct aaaaaagaca agatttcttt
    25921 ttctacagca ggtagatgaa tagctgaaaa gtcagttaat ctcatctcag ctttcctcgt
    25981 ttccgtcaaa atcaacttct tcatttgatt ctgtcatcat tttcgtcaag gtctcttctg
    26041 ctttagctaa tgtatcttgg caatgtttgc ttaattccat tcctcgttta aacgaatcta
    26101 gagctgattc taatggaacg tctccttgtt ctaactgcat gacaattttt tctaattctt
    26161 gcagtgactc ttcaaaagtt ggattttcca tatttttatt ctcctttgtc tatctcttgt
    26221 acttgtcctt taacgattcc atcttgataa tggatcgtca gcaaatcccc ttgatgaatc
    26281 tcggtgacac tttttactac ttgatcattt aacgtcgtat agctgtagcc tcttcccatg
    26341 attttcaatg gactcaacaa atcaagagat tgaacagctt gagcaaaacg ctgctgctgt
    26401 tgtttcatat attgttggat tttctcttct aagcgttttt ctagataagc agtttcttgg
    26461 cgagctgctt taactcgata aagcggagcc acttgttcca attggtggct taatgcgatc
    26521 gtttgttttt ctttcgcatg ataaatttgc tgtgtagctt gatataatcg ctgtttcaac
    26581 tggtctaatt tgatggtctg accttcatac aatctttcag gctgccgaaa tacataggaa
    26641 cgtcgagcac gctcgaaccg ttcttgtttg cgctggatct ggcgtacaaa cgcctgctgc
    26701 aatctagctt gccgttcttg aatgcgcatc aactcttctg acaaaacagg aacagctaat
    26761 tctgcagctg ctgttggagt ggcagcccga acatctgcta ctaaatcagc gatcgtggta
    26821 tctgtttcat gtcctacaga tgaaatcacc ggcgtattag cttgataaat cgcccgagca
    26881 acccgctctt cattaaacgg ccataaatcc tcaatcgaac cacccccacg accgatgatc
    26941 attgtatcga aattgcccat ctcttctact ctctggatat tcctgacaac attgtccgct
    27001 gcttggtttc cttgtacaac tgttgggaac aaaaccaatt ggacaattgg atatcttctt
    27061 ttcaccgtcg tgatgatatc gcggataacc gctccgcttg gactggtaag gaccgcgatt
    27121 cgctttggga aacgtggcag cattttttta ggcccttgaa aaaggccttc cttcatcagc
    27181 ttttcttttc gttcttccaa ttcttgatat aaagcaccta ttccatcagg ctccatgtgc
    27241 tctacataga tttgataaga acctcccgct tcatataatg aaatacgccc aacgacaagg
    27301 actttcattc cttcttttgg ctggaatcgt aatttttgga atgctccttt gaacataata
    27361 gcagatattt ttgcatggtc atcttttaag ctgaaatatt gatgggcatt ggcacgtaaa
    27421 cggaaattcg atatttcgcc tgttaaatat acgcgttcta aataaggatc ggcatcaaat
    27481 tttcttttca aatacttcgt taatgtagtg acagttaaat attcttgtgc catctattca
    27541 cctgctccaa aagcttcggc agattttacg gtttgataca tcagcatcgt gatagtcatt
    27601 ggccctacac cctttggcac aggagtgatc cagcctgcaa taggctccac ttcatcaaat
    27661 ttcacatcac cgatcagctt accgttagca tcacgattca tccctacatc tatcactaca
    27721 gcacctggtt ttacgaattc ttttgtgaca aaatgtccac gtcctattgc tacgactaaa
    27781 atatctgctt cttgtgccag ttcagccaga ttttcggttt tagaatgagc aacagtaaca
    27841 gttgcatctg ccatcaagag tagctgagcc atcggtttac cgacgatatt gcttcgtccg
    27901 ataactactg ctcgtttacc cgccggatcg atagaataag cttcaaacat tttcatgatg
    27961 ccgtatggag tacaaggaat cttgtccggt ttacccgcaa ggagtctgcc taaattcatc
    28021 ggatgaaaac cgtctacgtc tttttcaggg cggatagcta acaggatctt ttcttcatct
    28081 atatgctttg gcaatggtaa ttggaccaat ataccatgaa aactaggatc ttgattgtat
    28141 ttttcaattt ctgcaagtag ttcgtcttca gaaatcgtct caggataacg ctctaccaat
    28201 gaatggattc cgatttcttt tgctacacgt tctttatttc ttacatagat ttggctggct
    28261 ggattttccc ctaccaataa aacgactaat ccgggatgga taccattatc tttcaatttt
    28321 tggatttttt cagcaatctc tgcctgcatt ttttcagcta attcttttcc gttaatcaat
    28381 tctgccatct aaccactccc agatattttt tgtgttttca ttctaccata gaattttccc
    28441 gatttataac aggtttatga tgattcatag aaaaaagcta ggacttacgc cccagcttct
    28501 aattctttca atacattgga taaaacaccg ttgacgaatt tacgtgaacg gtcatcacta
    28561 aatgtttttg ccaactcgat tgcttcattt aaagcaacag ccgctggtac atctgtcaca
    28621 taagtcattt caaaaatcgc gatacgcaag atcaccaaat ccatttttga gatccgattg
    28681 atgtgccaat tgttttttaa atgtttttca atcactttat ccaattcatc tttttttgca
    28741 cagacaccgc caactagcgt atcaagataa acaggaacaa actctgattc atcttcgttg
    28801 atcatgtcgc ggtggtctaa ttcgattgcg ttgaaaatcg catcttcttt tgttaggtct
    28861 gcattgaagt ctaatggaaa taaagcctgc aatgccattt cccgaatctc gtgtcttgat
    28921 aattctttat tcattctctt cctcttcgtc ttcaaaaagt tcttcgattg ttggttgttc
    28981 taatttttca ggaacaacag ctactacatg gatattgact tcagataaat cgatatccgt
    29041 catgaaaagg acttgctggc tgacccgttc ctgcatttcc attgctactt taggaactga
    29101 aacaccatat tctagataac agtaaagatc tactttcaag ccatcttcct cgtttgctaa
    29161 gtaaacacct tttccgtgag ctgcacgtcc taataattca gtgacattgc tggcaaaagt
    29221 tccgcgcatt ccgtatacgc cttctacttt agaagctgca atcccgataa ttacttcgat
    29281 gacttcagga gcgatcacga tctctcctaa atcttggttt gcgtttaaaa tcaaattttt
    29341 ttcatctgcc atttatttat tcctcccttt gcctaagcac gagatacatc cgaattctct
    29401 atgatgcggc atagtgatca cgtggtaaga aaactgccgc cgatcagctg caaactgcta
    29461 tgaattggag aaaacaccca actttattat ctcttacagc ttaaaagttc ttctatagtg
    29521 tatcattaaa tcgagtgatt ttctataaca aagtctgtga acactgattt ttacgcttac
    29581 ttcttctatt gatgaaacta taagatgatc aattcttttg gtgaacgagt caacacacga
    29641 tttccatctt ctgtgatcaa caagtcatcc tcaatacgta ctccaccgat tcctggtaag
    29701 tagatccctg gttcatcagt gatcacattt cctggtacaa attgcttttc tgcacgagaa
    29761 gaaacgtttg gtccttcgtg gatctctaaa ccgatcccat gtcctgtaga atgtccaaat
    29821 gcttccccgt aaccatgttt tgaaatataa tctctagcaa ctgcatctaa ttggacacca
    29881 gtcattcctg gttttgcagc atcaataact gctaattgag catctaagac tatttgataa
    29941 atttctttta atttttcccc aggatcacca atagcgaatg tacgtgtcat atcggaaacg
    30001 tacccttcgt aatagcaacc aaagtccaat gtgatcaagt caccttgttc gatgattttt
    30061 ttgcttgcta caccatgcgg catagccgaa cgcaatccgc tagcaacgat cgtatcaaaa
    30121 gaaacacctg aagcgccaag agaacgcata taaaagtcta gttgatttgc gacttcaatc
    30181 tcagtcatgc ccggttggat cattttcagg atgtgatcat atgccatatc cgcaatatga
    30241 catgcttttt cgataatagc gatttcttct tcatctttca cttctctgag ttcttcgatc
    30301 atctgtgaaa caggtactaa ttccgcatcg atgatttctt ctaaaaccga atattctaaa
    30361 aatgagactg ttgtttcctc aaaagctaat gagtgcagtt cttctttgcg tactaagttt
    30421 gctacctcat caaaaatagg tccgacattt ttaataattt caaacccttg agcctgcgct
    30481 gccgcctgct cggtataacg aaaatcagta ataaaaaatg ctttttccaa agtaataaca
    30541 gctaatcctg tcgtacctgt aaagttcgtt aagtaacgaa gattgtacgg actggtaacc
    30601 aaaaatgaat ccaaattttc ttcttgcatt tttttgcgca atttttctac acgtagcatc
    30661 ataatgaaac tccctctttc tttgcacgct tttttccatt atagcaaact tttcttcgta
    30721 cacgacagtt aaataaaaat ttccggaaaa cttcttttta ttttaaataa ataaagccaa
    30781 aaaggagtca cagtctcgct gcaactcctt ttagtttact attttgtaaa aacttatgat
    30841 cgttttccaa agaaaaatga gacaactgct accaaaatca ctgcgcctaa aatagatgga
    30901 ataagtgcca tacctgcagc ttttggtccc cattcgccga aaatcgcttg tccaatactt
    30961 gaaccgacta gtccagcaat gatattcgtt acacagccca tcgaatttcc tttactggtg
    31021 atcgcacctg caattgctcc gatcacggct ccaacaatca atgtccataa ccaactcata
    31081 atataacctc cttctttgct aatcaatttt agcatatggg atttaaaggg gacaaataat
    31141 tggttttgct atttcatatt ttcttctgca aagtaaaaaa ataaagaaaa cctcctctta
    31201 gaagttttct ttatttttta cttgcgttta aacatactac gaataccacg tttcactaca
    31261 tcaaagaaac tcaatgattg taccattcta tcttcatcta catattctct gatcgcacga
    31321 agcacttttt taggttcttt ggaagaaaat gtgtatgtac catttttttt cgtttgaatg
    31381 gcatatcgag gaatccattt tccctttagc atgactgatg ctagtacata atccacttct
    31441 tcccatggaa tttggatata tttgcgtcta tctcgcgcat ggtaaaactc aaaaccatta
    31501 ttacctatca tgatttgccc gtaatctgtc agacccgtaa acgaagtagc atcaattacc
    31561 aagtctactt ttgtattgat tgattgaacc attttctccc tccagatatt tgtttatgaa
    31621 cagtatacta tataaaaaaa gctcgggaca acgcccgagc tatattttta tggattataa
    31681 aagtccgatg acgtgtccaa caacaccgac tacgaataaa ccaaggatga taacgattgg
    31741 agaaaccttt ttcttaagca accacataca taacaatgtt aatcctaatg cagctaaacc
    31801 aggaatcaat tggtctaagt tgtcttgtaa tgttgttacc ttttcaggag aaagggctaa
    31861 gccattatta acttgttcaa atgcactctt gattccttca ccacctgaag gcaatttatc
    31921 ccattcgatg taagcacctt tatctaattt tacagttgat acaactggta agaatttgat
    31981 tgatacccat ctttgtacaa gggcagctaa tacgaacatc cctaaaattg aagcaccttt
    32041 tgttacgtct tgtaacaatc cgccagaaag gtcatcagta attttagaac cagctttgta
    32101 gccaaattct tgtgtatacc acatgaatgc ccaacgaata atgttccaac ctaagaagaa
    32161 gatgattggt ccaaggatat ttcctcccat agctaatgaa gcacctagtg cacctagcat
    32221 tggacgtact gtaaaccaga acactgggtc accaacacca gctaaaggac ccatcatacc
    32281 tactttaacc ccttgaatcg ctacatcatc aacaggcgcg ccattcgcac gttcttcttc
    32341 cagagccaat gtaaccccta aaattggaga agcaatgtat gggtgtgtat taaagaattc
    32401 caagtgacgt tttaatgctg cagaacgttc ttcttttgtt ttgtataatt ttttgattgc
    32461 tggaatcata gaaaatgccc agccaccgtt ttgcatacgt tcgtagttcc atgaaccttg
    32521 aatgaaggta gagcgccacg caacagctaa acgatctttt tttgataatt tgattttttc
    32581 ttctgccatt ttattttcct ccttcaagat taatagtcat tcaaaatgtc gcctaaaggg
    32641 tcaccagagt tgcttccgcc tccattattt gatgaaccac ccattttaga aaggttcaag
    32701 taaataagag ctaatgcaac acctaaagca cctagagcaa tcaatgttaa ttgagaaatt
    32761 gcagctacaa cgaaaccaat aacgaagaat ggccatactt cttttgtagc catcatgttg
    32821 attaccaaag cgtaacctac agctacgacc attcccccac cgatagccat accatctgtt
    32881 aaccatgctg gcattgattc aaggaatgat tgcacagttt gtgctgggat aaatagcaac
    32941 gctgctgctg ggaccgcgat acgaacccct tgtaaacata cagcaatgat gtgccacatt
    33001 tcaaccttac ggatgtttcc ttcttcagca gccgcatcca tcaaatgaac gattggaaca
    33061 gcgattgtac gtacgatcat tgttaagaac aaccctgcaa cagccaaagg aacggcaatc
    33121 gcgatcgcag aaggaacacc tttaacacct tgtccaccta aaactaaaat aattgctgat
    33181 gctactgatg ctaatgcagc atctggtgct acagcggcac cgatatttgc ccaacctaaa
    33241 gcgatcatct gaagcgtacc gccaagtacg ataccagctt cgaggttacc ggtaactaaa
    33301 ccgatcaatg tacatgccac tagtggttga tgaaattgaa attcatcaag aataccttcc
    33361 ataccagcta gaaaggcaac taatacgact aaaattattg aaataataga catgataagc
    33421 ctcctataaa ttttaatatt atttattacg cttttgctaa ttcagctttt gcttttttca
    33481 aaatatcatc catgttatcg cgtgaatcat taggtacttt acgtacatcg aatttcacac
    33541 ctttttgttc aagcttctca aacgcttcaa cgtcttcttg tcccatagac aatactttac
    33601 ttacaacgac tttaccgacc gaatgcgcca ttgacccaac atttaattct ttgatgtcta
    33661 cgccaccttc aacagctgtc aatacgtctt caggattttc aaataaaagc aatgctttgg
    33721 tatttccaaa acgagggtct tttgctactt cgatcatttt gctaatagga ataacatttg
    33781 cttttacacc tggaggagca gcttgttcga ttagtctctt acgcagatca tcctttgata
    33841 ctgcatctga aacaacgata atacggtttg gttgcactgc ttttgtccaa gctgtcgcaa
    33901 cttgtccatg taacagacgt gagtcaacac gtgctaatac atatttgatt tttccgtctc
    33961 cgaccacagt tccttctgga agtgctcctt taggttgtgc gtcttcaact gctgctttcg
    34021 gtgcttctgc cggttctaat tcttccggtt ttacttttac accgtctttt gctgtttcaa
    34081 tgatgtgtgc tgcaatttca tgagcagatt ccattgaaaa gcgtgatgcg taggcttcaa
    34141 tcaacattgg taaattcaaa ccgctgacga ttgcccattt atctttgtgc tcttcgaata
    34201 atgtgtttga ttgattgaac ggtgttccgc cccataaatc aactaagaat aacacttcgt
    34261 cttgattatc aaaagaagca accgcttctt ccaatttggc tctcaagtca tctggacctt
    34321 cacttggttt caaaatcacg gctttgacgt tttcttgttc gccaaagatc attgaaccgg
    34381 attgtaagat tccttcagca aattgtccat gacttgctag gataatccct accatattta
    34441 tacctcctat ttacttttgt tgtaacaaat tcaaaaatat taataattgc taaccattac
    34501 aacagaatta tttgaccttt tccaacaacc attcttgcta cacctttttg attttcgcta
    34561 gtagttccat caaaaggatt ttcctgtcag caggtacttt tcgaatctct aactccactt
    34621 cttgactgtt caagtaacga aaggaatcaa tatcttgttg atttactgaa acaaaattgg
    34681 taatcattga tttgccttca gtaaaactca taccgccgat attcaacgac ttcaactgta
    34741 caccgcagcg gacaaccgct tcaacatctt ggggattggt gaataaaagc atcacttttg
    34801 ctcgtgataa taatccacta tgatagattt gaatcatttt ctgcacggtt attacatttg
    34861 ctgtgatccc cggcggtgca gcttctttta gtagaaactt tctcaattta tcttgagcga
    34921 ctacatcgct gatcacaatg atacgttcaa cacctgtttg cttcgaccaa actgttgcga
    34981 cttgcccgtg gattaaacga tcatctatac gtgccaatcg aatatccatc tgcaaaactc
    35041 cttcttcctc actacttcac agaaacccct atcatttctg tgtgaacaac cacctcctag
    35101 cacacactac tataagcaaa aagcgtgcca acttttttta cgatacacta attgaattta
    35161 aaggattctt aaccaataca caaaaacgat acactataaa agtgtatcgt ttttgtgtat
    35221 tcatggcaca gtgtattctt ttttatctaa ctgattatgg actagacgta gcagataata
    35281 catttcttct atcggcactt ttacttttat ctgatcttcc aacagaacaa ctgaggctgc
    35341 caattggcta taaagaggat cattcgttgt atttgtcaat tcgttttctt ccacagtcaa
    35401 tggttcattc cgaataatac gttcgatcat tcctgctgtg tgcatcacta ggttgatttt
    35461 gaatccatat tcttcatcgc cgattcccgt cgtctgagag atttgtgtag aaaattgcca
    35521 cattggatcg attaacttac taccattaat aaagatgaag ttatcagaga taaactcaac
    35581 acacgtattt ttagctgact cttcatctag ggagatgaat tcagtttcct ccaattctga
    35641 ttccatcagc aactgatcca aaatgacttc gatattttga tcaataaaac gttctaaagg
    35701 aataaatggg gcttggatat tcaaatcagt aatcccagta gtagctatga tctgatattc
    35761 tttttggata actggcagtt cttctttcac ttccacaatc gacaaagcta gaacttctaa
    35821 ttctgtttct tgacgcttgt ttaccgctcg ttcgatcaat tctttgattc tttgtgctgt
    35881 accttcgcca gatgcgcaga tagccaaaac agcttttttc ttatgaatag gttttgcttc
    35941 ctgaacagat tctacagaag atatttctgc gtaacctcga aaatttttca atgattcatg
    36001 caacatatct agatctgtcc ctaatactga gacctttcgt acagtttcca aaactagaga
    36061 agttgttacc atatcaacag tacgtactgg aattccagtg tctctttgaa tttgcgaatt
    36121 aaacgacgca agggacccca tatctactaa gagtatgacc ccgcttcctt catctacttc
    36181 ttgtacattg cgttcaattc ggtccaatgc tgttttcgga tccatatcca aaggcatgtc
    36241 tactgctcgt acgttctctg catctaacaa ctgttggaca acttggacca tactgcttgc
    36301 tgtgctgttt ccgtgagctg caacgactac accgattctg ccgcttgctt gatttgcccg
    36361 taacgagacg agtagaacgg tcaagtaata gtcttcactt tctggaatct ccacttgaaa
    36421 atacgtttcg atataactac gcacttcttt cgcaacagca tattcatctg gaaaatcaaa
    36481 agccatctca cggatgttat cattcgtatg ccgttcttct cctaagttga tttttttcaa
    36541 aaaagaactg atatgcaagc tcatagcata aataaagttt tgctgaaact catatggtaa
    36601 agatcttttg accattacat aaatctgatt ggtcacttct atgattttcg gatctacaaa
    36661 ttctgctaat ttgctgtctg cattaaaaga aaaaccatgg tctttataga aagacttcag
    36721 atggatgttg atatctgtag aaatgaaatg attgattgct tcttgatcca aaccatccga
    36781 cttcagtaaa gctgctttat ctccgatgat atcatataga ttataaggta actcatagga
    36841 gtccgattgg attttcatga tcgtatcatt tggtgctaca gttatctttg gctccaatat
    36901 tttagaaagc tctgccattg cactgcggtt gcttgctaat tgaatcaatc cgctacgaat
    36961 cccttctgtc aaatcatcga ttgtgatcga gatttcgggt gattgcatat gatttagaaa
    37021 tcctcgtgca catactaact ggatatttga cttcaattgt cctatatttc cataggtcac
    37081 actgccgatc aatgcttttg ctacatcttc tgtcaaagaa atctttcgct gcgtgcggtt
    37141 tgcttcgtgg gcaatcatga tttttactaa atcgactttc tcatttgcag gacgtttttc
    37201 aaaagccgga agtttgatat tgattgggat acgccgaaca aaagtttcta atagagaaga
    37261 acctgggtct tctgtggtcg cacctacgat ccgtacattt gcttcatggg atttcgttgt
    37321 ttccccaaga cgactgtaag ttccatgatc cataaagtaa aagatcattt cttgaccttc
    37381 aggcggcaac cggtgaatct catccaaaaa cagcataccg ccatccgctt gatcgataat
    37441 ccccattttt tcttcatctg cgcctgtaaa agcacctttt gcgtacccaa ataaatgact
    37501 catcaaaagc tcagggttat tggcataatc agcacaattg aaaacaatca gttccgtttc
    37561 ctcatctatt acatgatgcg cttttgcaaa atgatacatc gcgtgtgcaa aataagtctt
    37621 tcctgatccg gtaggtcctg taatcaaaca atttaatcct cttggcggat acaaaatcgc
    37681 agcttttgcc tgttccactg aatttttcat gctgccgtta gctccaatga tttttgcaaa
    37741 gatatcctta gtatctattt ggagtgtatt tttttcttca gagaccggcg tttctttgta
    37801 actgactaca tgttttgtaa ctggctttac gtgcgtaacg agtgtttgat atatgtaacg
    37861 tactggtcgt ccgtctgtct ttatcagttt tccttcgcgc actagctgat tcagatcttt
    37921 actgctattt gttcgctgaa tccctaacac ttcagcaatt tcctgtgttg ttactccttg
    37981 atcatattct gcctttgtta aatgagcggt tttttctttg acatacatat aaattcggtc
    38041 aatgcgtttt gccaatggta tcgctccttt tctatagact attgtactta ggcttcaaaa
    38101 cataatacta tgtatttatt tctgtttcgt caactattta cctgattttg ttttgttgat
    38161 aacacgcgga tagatagaaa tgttcaattt aaaagaacga aaaatcccga tacgattttt
    38221 ataatggaaa caagaaaaag aacactgttt accatataat tatttcactg atagcaaaca
    38281 atatagcttc catcaattga aaacgatagt tatcaaaaca tcctgattcc aaattggcaa
    38341 aaaaatatta agtgtaaaaa gacaaagtaa acttcgtagc ctccactaca acttttattt
    38401 ttataaacaa aaaaggctgt gacaaaagtc ttgtcacaac ctctttaatc ataagattca
    38461 attaagcttc ttgagctact ggataaacag acacttgttt tttgttacgg cctttacgtt
    38521 cgaaacgtac tacgccatca acttttgcaa ataaagtgtc gtctccaccg atacccacgt
    38581 ttacacctgg atagattttt gtaccgcgtt gacggtaaag aattgatcca cctgtaactg
    38641 tttgtccatc agcacgttta gcacctagac gttttgattc tgagtcacgg ccgttagatg
    38701 tagaaccgcc accttttttg tgggcaaata attgtagatt catagataac aacatggtct
    38761 gcacctccta tttatcatta attgttttaa tttggatata ttctgagttt tcagcttcta
    38821 tcgattgtaa tcctaataaa agattttcta atagaatctc agcaatattg ttttgttctt
    38881 gtgtcattcc tgaagtgact tcggtataaa gataaccgcc ttcttcttca ttcatttcaa
    38941 caatcggctc aaaaccggct aatgaggcaa tcccgttgac agtgctgatc gctaacgcag
    39001 aaacacccgc acaaacgata tcacttccat aaggaccagc atcagcatgc ccggtcaaag
    39061 tgaatgaaac gatctgacct gcgtcatttc gcttaaatgt tcctttgatc atatataggc
    39121 cttctttctc aaacgctatt aagcgttgat cgcgtcgata accacttttg tatatggttg
    39181 acgatgacct tgtttacggt gtgaatgttt tttaggtttg tatttgaaag taacgacttt
    39241 cttttgtttg ccgtgttttt caacagtacc ttcaacagtt gcacctgcaa cagttggagc
    39301 gcctactttt gtagattctc cgcctactaa gataacttcg tcaaaaacaa ctttttctcc
    39361 tgcttcaacg tcaagttttt caacatagat tgcttgtccc acttcaactt ttacttgttt
    39421 accaccagtt ttaataattg cgtacatgct ataaagcacc tccttctgat atacttagac
    39481 tcgccatccg aagtgacagc atatgctgtg cttattactt gttctgagcg gttgtagctg
    39541 tggagtccac aattacaaca gtactagctt accagaacag caaccgttag tcaactaaat
    39601 ttaaaatcaa aaagtaaaac aaaaattcaa gcaatacttc tgcttttcta tcctcacagt
    39661 tctaccaaat aaaatagtcg cattgtgttt cttttattct tataataaaa gaaaaaggag
    39721 gagcataaat ggaataccca aaagaactga tacaagaagc ttctttaaga atgaacggcc
    39781 gtcctgtaga aggatttatc gctgggcaag gacctcttca tcctaaactg atgctcgtgg
    39841 gagaagcccc agggaaaaca gagattgata cgcatgttcc ttttagcggt caagctggaa
    39901 aagaattgat gcaggcactt tcttcaactg gcttaacacg agaagaggtg tatatcacca
    39961 gtgctgtaag gagccgcccg tatcgcgtga ctcatcggat caataaacgg acacaacagt
    40021 cagaaactgt ttatccaaat cggacaccta ctcgttccga agtatttgcg catgcaccaa
    40081 tattggatta cgaactgatg aaggtccagc caaaattaat tgcgacactt ggaaacattg
    40141 gtcttcaacg tttacttggc aaagaatata cggttaccaa aaatcatggt caactctata
    40201 cagggcctgt cttacaatta acagaacaaa aggatgccta cgaatggtca gaaaaaaact
    40261 atcgaattct tccacttttt catccagcag ctattttcta caatcgctcc cttgtacctg
    40321 ttatccatca agactggcaa gtactagctt cacttttaca gcaatattaa aaagaacaga
    40381 caagaccata aaatcttcag tttacagtct tgtctgttct ttttcattgt tttaatggct
    40441 aattgcagta tatttcgcat gtaaagctgt caacgcatga gcaactgttt gatgttgatg
    40501 cgtgaatgta tgttcccaca agtgagaaag ttgtttttct acttctggct gcaaggaagt
    40561 atagataatc gtttgtcctc tttgctctgc tttttcttga aattcatcaa aaactgcacg
    40621 tgccgtcaaa tcaatacttg gtacgcctcg catggagaaa atgacaccat cgtgatgatt
    40681 tagttcttcc aatttatttt tgagtttttc tgaagacata aaaaacagtg gaccactaat
    40741 atacactact gtccagttat tcagcttttc cgtttctggc agccccattc tttgccaatc
    40801 gatcgtttct aatgaaatcg tgatagcagc gcttttgaca acaaaaaata cacagccgcc
    40861 gatcaccccg atcacgatag caatacttaa atcaaagata actgtacaaa tcatcgtaag
    40921 gataaacaat agaactgctg accaatattt attagtaaat agctccttga tcgtttccca
    40981 ttcattcata cgccaagccg tcacaatcaa aacacctgca agtgctggca ttggaatctg
    41041 tgacatcact ggcgcaaaaa caagcatgga gagcagtaag acaactgcat gtatcatacc
    41101 agctaatcta gtttgtgcac cagatttgat ggccacgctt gtacgggcga tggccgcagt
    41161 agcaggaatc ccgccaaaga aaggaagcaa cagatttcca attccttgcg ccattaattc
    41221 ttgattgcta tctaagggct tatttgccat tcggccagcc gaagcaccac atagcaagct
    41281 ttcaatcatt cctaataaag caatgctaat agctggaact gccacttgtt gcaaggcaga
    41341 aaagctaaaa tctcccatat tcaaacgatt actactgatc aatgtttgtg gaatcttacc
    41401 cacagttgct actggtaaat ggaacaatag cataactgct gttgccaaaa tgatagccag
    41461 cagcgaacta ggcattcgtt tcgcccattt ttttggatag ataaacatcc ctaaaatgac
    41521 tagccctccc atgatcaaag aggctgtgtt tatcggaaat cctatcgttt gataactact
    41581 tagtttttca ataacgtttg taccttccga gtgaacacca aataaattat cgatctgacc
    41641 taatgcaata atgatagcaa tacctgaagt aaaccccgta attacgggtg ctgggataaa
    41701 agaagtcagt gttcctaatc ggaaaagtcc tgctgcaagt aaaaatagac ccgctaaaaa
    41761 agtagcaagc aatacacctt gcattccctt tgttgccgca atagacatca agatagctgc
    41821 catcgcacct gtcggacctg atatttgata aaaaccgcct gataaaccgc caataacgat
    41881 accggcaatg atagccgtaa tcaaacctgc ggccgcatca gctccgcttg ataccccaaa
    41941 agccaaggct aaaggcaatg ctacagcagc tactgtgatc ccagccaaaa gatctttctg
    42001 aaatcttgaa agatcataac ctgaaaattc tttttttagt gaggaaacat aatttttgaa
    42061 cacctatcta actccttttt catgaaattt tctaaaatac cattcattag aatatcatat
    42121 ttcatgagat aaagtaatat ttttcacaaa gtttcctgtt agataaattc ttattttttc
    42181 tatatttata agaaaataga aagcaatgcc aacagagcta gcccaccttg tttgagtaaa
    42241 atccaacgat cgctagtcaa actgccatat aatgcaacaa aaaaaataga tattaatagc
    42301 atacttgtga tttctttaga tgcggaagaa agataagctc cataaagtag tcccactcct
    42361 aatagtccat tgtacactcc ttggttctta aacaatgttt gaacagattt tctgcttaac
    42421 tcttctttac tcattttaaa aaccctactt gtcgtgtcag aatcagttct caccgtttcg
    42481 atatacatga tataaaaaaa ttcaattgca actagagtca ctaaaattct agatagtatc
    42541 gtcatatctt cttcctcttt tctgatgagt tttatttttt tgctccttat aattgtactc
    42601 gataaggatg tttttttatt ttatttgcct ttgaaaacaa acaatattgg cacaaaaaac
    42661 aaagaaaaac cgttagaaac ctcagtttct aacggttttt tagcgtcaca ggagggattc
    42721 gaacccccga ccgtccgctt agaaggcgga tgctctatcc agctgagcta ctatgacaag
    42781 aatcagcgga acatcaataa ttatagataa ttaaattgct tctgtcaata cgaaatagtt
    42841 atttaattag caaataatga attattttgg cccaactact aaatcaatct tttaaaacga
    42901 gacattctcc ttcagctgtt ctaaggttat caattgaaaa caggagctac attgacttca
    42961 tgctactcct gtttatttag atttcctgta tcctttaatt gacttacaaa tatagaaact
    43021 tttacatcaa gttcgaattg agccctcgat tcaatcgatt atctacaggc tgccataatc
    43081 atgtatcaca actcgattgt ttctgattcc tttgtcatta agatcacacc atcacctaat
    43141 ggcaagattg tcgatgtcaa atcgggatga tccatcacaa cacgaagaaa ttcatttaat
    43201 ttacgatgga tagctcgttt gccacgggga atctcttcat ctgggagcaa gatcgttcct
    43261 ccttggaaga tgtcatctac catcaaaacg ccaccttttc ttaacaaacg taagcattca
    43321 ggtaaaaatt caatatattt tgattttgca ctatccataa aaataaagtc atacggacca
    43381 gacaactccg gcaagatatc tgctgcttga ccttcaagca aagtcacttt atctgtcagt
    43441 cctagacgtt catagttttt tttagctttc tcgatcatga catcgaaacg atcaattgtc
    43501 gtaacatgag cgttttctcc gattgtcgtg gccatgacac ttgatgaaaa accgatagct
    43561 gctccgatct ccaagattcg ttcaggctga agctgtccaa gtaaaaattt caaaaataca
    43621 actgtttcat ggggaataac tggtacttct gctacatgag cttcttcttc cagtttgcct
    43681 agctcacccg gtaatttttt ttgctgggta cgcataaaat ccaatagttc ttctttgact
    43741 acaggacgat gcatcatttc attgcgcata ctactcctcc tcttcacaca ttatacgaac
    43801 aactgactaa atctacaagc tgcatgagtg ttttctcgtg tttcatgttc agaagagtcg
    43861 ttggatctct ggaatacatt tatgttttta acattcttac aaaaatttct gtgttcgtct
    43921 ccgcttcttt ttcaactcta caaaggtatt ccatgctgtg attcctgtta ggcaaaaagc
    43981 aatcgtaaag aataacccct gaatccatgc aaatgccgtt tttgaaagga ctagtccaat
    44041 atatggaatc tgaaagatgg gactaccgat aatatttctc ttttttacta ctccaggctc
    44101 aatataagtc tttgtatcag cttttgtata aaaatcttcg tggttgtttt caacggctat
    44161 tacgcgacgc gtcgagattt tttttcctga attttcataa tatgtaataa tatctccgac
    44221 taaaatctca tttgtatttt tcggtcgtac atatatcaga tttccttgtg cataatatgg
    44281 actcatttct ttgtcctcaa taatataagg agtcaaacca aaaattcggg gaaccaagac
    44341 gacgagaaga ataagaatta aaaatataat caacgttata gaacttataa aagttatttt
    44401 tttgtaatca ggtatcgttt tcaacttctt tctttattca atccttctta atcatataat
    44461 aacgataaat tttttcaaat tatagctgta aaaatgtttc cctttaataa ttatatgttt
    44521 ctaattctat ttcctcatct ttatctcatt ttttattata aataattgac gttagtttaa
    44581 aaaacgttta ccctaacatt acaagtttta gaaaggaata cctttatgtc tttttttaat
    44641 aacatatata ataaaaaaga atctttttat ttacgtataa agtggagcat aatcattata
    44701 ttatcattgt tatttttaac aggaattgct tatttctttt attttatttt ttctcctgcg
    44761 gacagcaatc aaatgaaaga aataaaagat tcgtgggttt tctatacaaa agatgatcct
    44821 gattttaagt ttgacagcaa gtacactcgt acgattccta ccgtggataa agacgaaaca
    44881 ttcattatgg aaacgacact tgaaaaaccg ttggacgaag caaatttact aatcaaagga
    44941 aaccatcaat ggattactgt atatctaggc gaaaagattt tataccaacg gaaagattat
    45001 caggaagaaa gcaatcccgg actctcattg gctgtgatag atttaccagc aaattatatc
    45061 ggaaaaaaat tgacccttac agtctcatct ccttatcaga actatgctgg actgccaccg
    45121 aaattttatc taggaacaac tggaccattg attagtttta tttattcaca atcagtgccg
    45181 caagtattga cgatgataat cgctgttttt atcgctatag ggaccttttt ctatacgatc
    45241 tacttgatgt ataaacaaaa acgtcttgaa tactcactta ttctcttaag ttgctttgct
    45301 ttggcattag gatttgaagc tgtatcagaa gatattttaa gtggtattct ttttgaacca
    45361 ttcgttcatt cttttttatc tcatttattt attatcttaa cgtcagcttt catcatctgc
    45421 tactattgga gccgaatgtt ttactataaa aaatggtacg gaattttttg tacgttccaa
    45481 cttttgattt ttatcggcgt tctgctttat gcaactttta ctcctgcaga acttcctgaa
    45541 ttaatgccga ttgctaatat tgctagtgtt ataagcactt tagtcacttc acttgcatgc
    45601 attggcgaag catataagaa taaccgtttt tatgtagctt gtacaccttg gatcgtcttg
    45661 gtagccatta tccattgctt gatttacatc caaacggcat taggtatcta tcaaacaacg
    45721 atcaattggt ctacgatttt atatattatc attctaatcg ttattatcag ctacaatcta
    45781 atcgatcaca tcttaaatac cgacaaagat cggagacaag tagatttctt aaaaatcaaa
    45841 aatgatttgt tggagcaaca ttatgagcag cttcgacagc atatccaaga agtaaataca
    45901 cttcgcctgg aatttgtcca agagatggag aatctgcaag atttaatgca tacggaccaa
    45961 acaaaagcta aaaactatgt ggaaaagata ttagaagaag caaaaaattt cgaaatgctg
    46021 tgttcttttt gcaaccacca ggtgacaaat cttatcttag cgcgttacca gaagttagct
    46081 gccaaacgga atattcagat aaccttccaa gcagatcttc ccgaagaact tcctatcaac
    46141 gatgacgatc tggctcaact tttgatccac gctttagagc attcctttag agaaactcat
    46201 gccatcgaaa atccactgta tcgaaaaatt tatttgtcga tacacgaaca agaggaacat
    46261 ttcgtcattt gttgcgaaca tagtgcacat tacgatacaa acctatttag tcgaggcatc
    46321 acagaagaat tagatgatca ggaacgtttt gacttaatga tgatgaaaga cgtagcggat
    46381 cgttacacag gaacactaac gcaggaaaaa gatactttca ttgatcggat cactgttcaa
    46441 ctctctcgaa attacccgaa tcctgtataa aaaatagcgg aatccatata tgtggattcc
    46501 gctatttttt attttcaata aagtaaggta agcaattctt ctggttctat cgcaccaaaa
    46561 tatttcttca catcaatttg gctgactatt tttgatagag atgctttatc atatttctgt
    46621 ccaaccaatt ttttttccac gtcagagata tctcctagtc cgaagaaatc gccaaagatt
    46681 tttgcttctt tgatcattcc ttctgagaca ttcaaatgga cttcaatcga accgatagaa
    46741 aaacgttttt ggcgagagaa attgaaatct ggagacttcc cgtaattcca atcccaattt
    46801 cgataatatt cctcagagat tcgattgatt tttttccaat cttcatcagt caattcgtac
    46861 gtttttactt cttctactga ctctacgccg aaaattttca acaagatttc ttgacgaaat
    46921 tcttctgttg tcattttttg cttttcttct ggaagaaatg gtttgatatt cgttacacga
    46981 gaacggatcg atttgattcc ttttgattcg attttgtctt tttttacttt taatgcattg
    47041 actacttcat tgatatcaga gtcgaacata attgttccat gagcaaacat ccgaccgttg
    47101 gtagcataca tcgcgttgcc agaaaatttc atgccatcaa tgactagatc atttcggcct
    47161 ttcaattccg ccccagcaac acccaattca tgaagtgctt ggatgattgg ctgtgttact
    47221 ttcgcaaagt cacggaatga atccccatca tcaggcatga taaaactaaa gttcaggttt
    47281 ccaaaatcat ggtagacagc tccgcctcca cttaagcgac ggacaacatg aatgccattt
    47341 tcttccacat aatctcgatt gatttcttca agcgtatttt ggtttctgcc gatgatgatt
    47401 gaaggttcat tgatataaaa gagcaaaata ggttcatcta aaggcatttc ttgcagcaaa
    47461 tattgttcaa ttgctaaatt gattcttggg tcgtggtttt catttggtac aaaaatcatg
    47521 ttcttcctcc taaatgttaa acactttgtc aactgcagca agttctatat caatcccttt
    47581 gcctgctgcc ttttgcgctt catttttcaa ttgttctaaa tcaccaaact gaggtaggtg
    47641 tgtaagtaca agtttcttta cgttagctga tttcgcaagt tctccagctt cttttgaagt
    47701 gaaatgggca tgatgatgtt catttccttc aaaaagatag gtatctgcta gaaacaaatc
    47761 agcatctttc gcaaaatcag cgaaactttc caaataacct gaatcacctg taaatacgaa
    47821 tactttcccc gttgtttctt ctacgatacg catggcgtaa catactactg gatggattgt
    47881 ttccataaaa gtaatcagaa aaggaccaat ttttaattct tcagcttcaa aataaggatg
    47941 tccttttgag acattttcca acgtcaattc ttcaaagtga atggaatctt ttgtgtgtcc
    48001 ataaattgaa agcaatgtct ccggatttct cttaggatga agttgccagt aatactgtaa
    48061 tacaccaagg tcagcaatat gatcatgatg ataatggcta aggatcacag catctagatc
    48121 aagtggatca aggtgttctt gtaatttgat cagcgtagca ctacctgcat cgatcaacaa
    48181 attgaacccg tcagattgaa gcaaataacc tgttgtccct tgatttttat aaggatacgc
    48241 tcctaagcat cctaaaacag ttaatttcat cgtacattcc ccctcaaaga tttatcctac
    48301 cacaaaagtt atttttttga tgaataaact gcttacatgc taagtaaaat aggcgtctgg
    48361 acctcctttt tcaatccgtg cttgttcata aaattctgca actgacacat tcacataaac
    48421 aatgacccac aaaagaccga tgaacaaagc gattgctcct agcaaatacc agccgataaa
    48481 agacagctgc aacaaaatat acgtaccaat tctatctttc aataaaaacc agccatattt
    48541 caaacattga ataaccgaca aatcaggata gtcgaacttt gtccatgcag caaattggaa
    48601 taaaccgcca acaatactca tgatgagtgc tgaaacaaaa atcatcccta tcatcgataa
    48661 aacaaagctg atatcaagaa aatcttctgg catgacaccc gtaggtaccg catttgaact
    48721 gaaaagcagc tgcatataag tactcactcc gctgattaaa aacatgggca gaaagattaa
    48781 actgttgagt agccagttga tgactgaatt gatcaaattg atgccaagta tcgaaccaaa
    48841 atactttctt tggaaaactg aaaataaaag gttacttttt ggtctgccac cccgtacaac
    48901 tactagcgca atgtaaaaca atgcatagtt gaatgcgaat aaaagtaaat tatcgactag
    48961 aaaactcaat aagtgctgct gcatgctttc ttccggaaaa ggactgaaac cgtcaaaaaa
    49021 gttctggatg acgctcgtaa atattataga taagaaaaca agccaagtat tgattcccca
    49081 ttgattttcc aaagaaactc gtgctttttt ccgatattca gaaataggta ttctattatt
    49141 cattattcat aactccgaat tttttcaact gtttcactat caagccgttt cactaattct
    49201 gtgactagtt tcactgtatt caaataatca tcttcatgaa tcactgatgt atgggaatgc
    49261 agataacgaa caggaacggt aatggctaca gacggaacac catctcttgt caaatgcatt
    49321 tgtcctgcat cagttcctcc gccagaaatc actgtatatt ggaatggaat atccatttcc
    49381 tctgctacat caataacaaa attcaataat tttttatgcg ggatcataga tgcatcaaaa
    49441 atcaaaatct gtgggccttt tcctaaaatc gaatctgctt cttttggtgt cattcctgga
    49501 gtgtctcctg ctgtaccagt atctaaagca aaagcaatat cgggatttac aaggtgtgta
    49561 cttgttcgag cacctcgcaa gcctacttct tcttgcacat cgcttcctgc gaataaaaca
    49621 tttgggtgtg cttctttaga tagattctct aatactcgca gagaaacagc tgtcccgata
    49681 cgattatccc atgcttttgc cagtaaatat ttactgccat tcattcgttt gtattcaata
    49741 tatggagtga ccatatctcc aggacgtatg ccccactctt tggcttcttc ttggcttgat
    49801 gcgccaatat caatgaacat atcctttatt tcataaggtt tatttcttac ttgaggtgtt
    49861 aaaacatgtg gtggtttgga accaatcaca ccgtggacga ttttcccttc acgtgttttg
    49921 atttcaactt gctgagcaag catgacttgc ccccaccaac ctccaagagt ttggaattcc
    49981 aagaatcctt tttcggttat ttttgttacc ataaaaccaa cttcgtccat atgtccagaa
    50041 ataaagacct tggggcctcc gccagcacca tgtttggcaa tcacactgcc taaaccgtca
    50101 taaaaaatat catcagcgaa tggtttggca tattctctga atacttctct tacctcttct
    50161 tcatttccag gtactccttt tgcagaagtt aaatcaataa gtagctgttc ttcttttgta
    50221 tccaaatgtt ttcccccatt tctatattca tattgtgatt ataccattgc ttatgatttt
    50281 tgttcaggca aacttggtat ttttatcttt tattcagaat attcaaaaat aaagaaaaca
    50341 agcgagcaac actcgcctgt tttctttatt ttaattgtag tttatctggt atttgatgtt
    50401 gccaagattt gctaaaaaaa gcatcgtcaa gatgatactt actagcagta atatgatttt
    50461 cttctaaaaa cggtctgatc tgttgatctg cagccaacca gcctttccag cctagatgga
    50521 tcgtgtcttc cataaaatag ttggtacccg cttgattgac aaaatcagct attcgattga
    50581 atccttggct atttaattga aatttgatct ttttggcaaa tccttgtaac atttcttgag
    50641 atagtccggt ataatcgctc catttctcgt taaccggtgg gatgatgaac aatacttcgt
    50701 tatggttttt tgctaattga tccaagacca gctgaaaatc agaaaattct ggcgaaaaac
    50761 gataatccca atttttttgt gactgtttga gctctggctc gtgggctcgg atacgatgcg
    50821 tataaaaatc gtttttcaac ccgaatggat tattcgtcgt ttctcgttct ccaatttgat
    50881 ttgctaaaat ggataattct gtttcttggt attgttttgg taatcgtttt gactcatgat
    50941 cgattttttg ctgtcgatct ttcaacccaa tattgctgaa tacttcatcc tctctcttca
    51001 gcatgttcca ttggctgcgc agctgattca aagattcagg taaaggtagc ttcccttttt
    51061 tgatcgtttg cagaatcgcc gtcaacgtat cattctcttt tacttttgag aaagttaata
    51121 atcttctagc taaatagcga tcagcaggcg ttactttctt cattgaaaac aaccagtcgt
    51181 atgtttgaag ttcagaataa tacgtattaa agtaatcagt ttttacacca tttttaacga
    51241 accactgagg agaaataata aagacgactt tcttgttttt catagcatct cctgcagaac
    51301 gcatcatcat atattggctt aacgattgtg tcccgggtgc tcccagcaaa aatggacgat
    51361 agtttcgctg gtatttttct gccagaacag acggatgaaa tggactgatt ctgcttaatt
    51421 cacttgagcc aaaaaaagga atatattgtt tttcagataa tgcataattt tttatcgcat
    51481 ctcctttcaa cacgttcact gacatggaag aagaggcacg tctaatcgtt tctggattag
    51541 agatatctac ttgataggag gaggtaaaaa acacaaacag taagccaaca gctaaaacaa
    51601 ccgggccgaa aatagcaaaa agtttctttt tcatactcaa cccttcaatg cagttgcttg
    51661 ttgaatgatt ttattcggtg tgcaccaatc ctctcgctcg aattcagata cagggacttg
    51721 gatatccagc tctccgtcta gctcgactaa taattgaaca gatgctaaag aatcaagcaa
    51781 cccttcatca aatagatcaa tatcacgatt ttttcttact tcatctgttc ctgttatttc
    51841 ttctaagatt gataaaattt gttcttccat tttccttttc tcctttgtta tataaattaa
    51901 tatgtcacta ttcgttagct aaaaaaaagt ttatctaaaa atcctgaaaa aatcaggaag
    51961 ctaaaacata ctgcttgaaa tgtcaaaaat acggctaagc catgcgtaaa tttgttagaa
    52021 ggcaactgct ctttatgctt cttcttaaat cggatccatg catctgtcag acagatcaga
    52081 caagcatgat acagtccata tacgatgtag taccacgtca atccatgcca tatacccatt
    52141 agaagaaaca aagcaaaata accgatattt gacattacga tcttgctttt aaaaaatttt
    52201 ttcttcatca tgaagaacat caatcgcata tatacataat cacggaacca aaaggacagc
    52261 gtcatatgcc aacgattcca gaattctttg atattccatg ataaaaaagg tttattgaaa
    52321 ttgattggtg tctgatatcc catcatgtag cttgtaccga ctgcaaataa gctatagcca
    52381 gcaaaatcaa aaaacaaata catactgtac acgtacatat aacctactaa tgcccatgaa
    52441 ataccaccat gctgtaaggc aatgcgtccc actacaggaa gcagtacttg accaaaataa
    52501 taaccgataa tgaatttata taaaaaaccg acaaaaatat tttggacgcc ttttcccaaa
    52561 agatcaacat atttatcacg aacaggcgga tggagctgat ctttttcaaa tctcctatag
    52621 cgatcgattg gtccagaaga gattgttgga aagaacagca gaaactggat ataacgaaat
    52681 ggcttatatg ttttgatcat gccatctctt atttccatga tcatttgtac agatttaaag
    52741 gttaaataag agattcctaa aaaaccaagt aacgatgctt ttccatgttc aaaaaaagga
    52801 gtcactttcg ttaaaaacag tggaattatc gctaaaataa cggcggcaaa aaaccatcct
    52861 gtctgatttt tcctttgccg ataaacaaaa tagccccata caagtatcgt ctgccacaaa
    52921 acatacccaa tcagcgccgc tccctgttgc caataattac ctccaaagct gacaaataaa
    52981 aagaaaagtg tcactgctga ttgataaact ggtaatcttt tttcccatat caataaagat
    53041 agaacgatcg gtacaagcgc aatggctaaa agaaaaaaat aaaacggctc tgcataaggg
    53101 atcatatgag gtaacatcat tcaggattca cctcagctgt taattttttt cgatcgattt
    53161 ttccattaac tgtctgaggc agcagatcga cataatggaa cttctgagga atcatataat
    53221 ccattacaac ttctagtaat tgttctttga tagatttagt caactgaaaa tccggctgtt
    53281 tcgttttgtt ttctaatacg acaaaagcaa ccagctgctg tacttttgtt ccttggtatt
    53341 ttggtacgac gacagcttgt ttgattcggt tgtcttggag cagataatgt tcgatatccc
    53401 cgagttcaat ccggtatcca tgtaatttga cctgctgatc catacgtcct tcataaaata
    53461 aaagcccata ttcatctagt ctacctgcat cccctgtatg gtaacttgct aagtcatttt
    53521 gacaaaagaa cgcagcagct gtcttttcgg gattgttcag gtaacctttt gatacgcttg
    53581 gtcctgcaat aatgatttca cccgtttctc cttttgaaac aggtttacct tcttggacaa
    53641 tagaaacttt cgtgtctttt tttacgtagc caatcggtag tcggtcataa ctatctagta
    53701 catgctggtc aatctgaata cttgaaatag ctacagttgc ttctgttgga ccgtaagtat
    53761 tatatatccg tgcatttggg aatcgatcaa gtagactttc tgcagttttt ttcgtcaatt
    53821 cttctccgca aaataaaaac atgtccaatt ccggcacatt ttctccattg aatgtaggtt
    53881 ccatcaagca aatatccata aatgatggag tagaaaccca cacattcaac ttcaaggctg
    53941 gcaatgtcgc gaataattga cgaaagtctt gaacaactgc tttatttaac ggcttcagca
    54001 ttccgccaga aactaatgca ggataaatag aaaataccga taaatcaaaa gaaaatggcg
    54061 cttgggctaa gaaatgcata ccaggcttga tcccaaagtc ttccatcgtc cattgaacaa
    54121 agctgactag attatcatga ctgatttgta cgccttttgg ttctccagta gtaccagaag
    54181 taaaaataat ataaaaattt tctgtacctt ttacgctatt tctgggataa aatcctactg
    54241 gtatcttctt gatttcttga aattcttttt ttgttatgac aggaaccgaa ggaatatctt
    54301 ctatccagtc cccgatactg atgaccatag atggtttagc cacccgaaag atcgatagga
    54361 tacgctcctt gggtgtatgc tcttcaatcg gcaaataggc atggccagat ttgacaaccc
    54421 ctagaaatga gacaatcatt tcaaactcca aattgccaaa aacgacaatt ggtccatcgc
    54481 cttctacctt tttttctaaa taataagcaa gtgcgtctga tgcttgtttc aattcaccaa
    54541 aagtatgcgt ctcatccgcc tgataagcaa ctcgatcagg ttcatttatt ccccaatcat
    54601 cgattgcttc aataatcgtt ttgatttcca tactcttcct cctaaaattc gttatagata
    54661 aaagttcctc cttgaatatt tttatagtga tacagataaa tcaggaacag cagtactgca
    54721 ctataaaaaa ttgttcgaat gatgaatttt ccccaataat tcaattttgg attagaacgc
    54781 catttgttca ttggattccc tcctcatata acctcacaca aagtgtaact aattttgaca
    54841 aaaagaaaca tgtgtctagc ttacagaaac cgttttttta ctaacagttt tgtcacatag
    54901 cttttataaa aatagcacat caaat
//
LOCUS       DAZKCE010000004        54643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000004 DAZKCE010000000
VERSION     DAZKCE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.004"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..173)
                     /locus_tag="ACG8KQ_000205"
     CDS             complement(<1..173)
                     /locus_tag="ACG8KQ_000205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103181.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINM"
     gene            complement(504..737)
                     /locus_tag="ACG8KQ_000206"
     CDS             complement(504..737)
                     /locus_tag="ACG8KQ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9103182.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(752..1702)
                     /locus_tag="ACG8KQ_000207"
     CDS             complement(752..1702)
                     /locus_tag="ACG8KQ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP9103183.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1699..2541)
                     /locus_tag="ACG8KQ_000208"
     CDS             complement(1699..2541)
                     /locus_tag="ACG8KQ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFP9103184.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2543..3262)
                     /locus_tag="ACG8KQ_000209"
     CDS             complement(2543..3262)
                     /locus_tag="ACG8KQ_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9103185.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3810..4100
                     /locus_tag="ACG8KQ_000210"
     CDS             3810..4100
                     /locus_tag="ACG8KQ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103186.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            4172..5398
                     /locus_tag="ACG8KQ_000211"
     CDS             4172..5398
                     /locus_tag="ACG8KQ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9103187.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(5486..5878)
                     /gene="rpsI"
                     /locus_tag="ACG8KQ_000212"
     CDS             complement(5486..5878)
                     /gene="rpsI"
                     /locus_tag="ACG8KQ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9103188.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5894..6322)
                     /gene="rplM"
                     /locus_tag="ACG8KQ_000213"
     CDS             complement(5894..6322)
                     /gene="rplM"
                     /locus_tag="ACG8KQ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9103189.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6856..7323)
                     /locus_tag="ACG8KQ_000214"
     CDS             complement(6856..7323)
                     /locus_tag="ACG8KQ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9103190.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7443..8981)
                     /locus_tag="ACG8KQ_000215"
     CDS             complement(7443..8981)
                     /locus_tag="ACG8KQ_000215"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9103191.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9061..9747
                     /locus_tag="ACG8KQ_000216"
     CDS             9061..9747
                     /locus_tag="ACG8KQ_000216"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9103192.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9948..10475)
                     /gene="msrA"
                     /locus_tag="ACG8KQ_000217"
     CDS             complement(9948..10475)
                     /gene="msrA"
                     /locus_tag="ACG8KQ_000217"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9103193.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10559..11236)
                     /gene="pgmB"
                     /locus_tag="ACG8KQ_000218"
     CDS             complement(10559..11236)
                     /gene="pgmB"
                     /locus_tag="ACG8KQ_000218"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9103194.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11233..12363)
                     /locus_tag="ACG8KQ_000219"
     CDS             complement(11233..12363)
                     /locus_tag="ACG8KQ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9103195.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12363..14546)
                     /locus_tag="ACG8KQ_000220"
     CDS             complement(12363..14546)
                     /locus_tag="ACG8KQ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9103196.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14543..15568)
                     /locus_tag="ACG8KQ_000221"
     CDS             complement(14543..15568)
                     /locus_tag="ACG8KQ_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9103197.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15613..16440)
                     /locus_tag="ACG8KQ_000222"
     CDS             complement(15613..16440)
                     /locus_tag="ACG8KQ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9103198.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16437..17504)
                     /locus_tag="ACG8KQ_000223"
     CDS             complement(16437..17504)
                     /locus_tag="ACG8KQ_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9103199.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17530..18366)
                     /locus_tag="ACG8KQ_000224"
     CDS             complement(17530..18366)
                     /locus_tag="ACG8KQ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103200.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18363..19232)
                     /locus_tag="ACG8KQ_000225"
     CDS             complement(18363..19232)
                     /locus_tag="ACG8KQ_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103201.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19302..20585)
                     /locus_tag="ACG8KQ_000226"
     CDS             complement(19302..20585)
                     /locus_tag="ACG8KQ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9103202.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20599..22284)
                     /locus_tag="ACG8KQ_000227"
     CDS             complement(20599..22284)
                     /locus_tag="ACG8KQ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9103203.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22559..23584
                     /locus_tag="ACG8KQ_000228"
     CDS             22559..23584
                     /locus_tag="ACG8KQ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103204.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24179..27832)
                     /gene="rpoC"
                     /locus_tag="ACG8KQ_000229"
     CDS             complement(24179..27832)
                     /gene="rpoC"
                     /locus_tag="ACG8KQ_000229"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9103205.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27911..31528)
                     /gene="rpoB"
                     /locus_tag="ACG8KQ_000230"
     CDS             complement(27911..31528)
                     /gene="rpoB"
                     /locus_tag="ACG8KQ_000230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9103206.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAEDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32256..33224
                     /locus_tag="ACG8KQ_000231"
     CDS             32256..33224
                     /locus_tag="ACG8KQ_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9103207.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33242..34147
                     /locus_tag="ACG8KQ_000232"
     CDS             33242..34147
                     /locus_tag="ACG8KQ_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103208.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34144..34950
                     /locus_tag="ACG8KQ_000233"
     CDS             34144..34950
                     /locus_tag="ACG8KQ_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103209.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35107..36075
                     /locus_tag="ACG8KQ_000234"
     CDS             35107..36075
                     /locus_tag="ACG8KQ_000234"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9103210.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36392..36916)
                     /locus_tag="ACG8KQ_000235"
     CDS             complement(36392..36916)
                     /locus_tag="ACG8KQ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9103211.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37203..38900
                     /gene="scm"
                     /locus_tag="ACG8KQ_000236"
     CDS             37203..38900
                     /gene="scm"
                     /locus_tag="ACG8KQ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9103212.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(39055..40041)
                     /locus_tag="ACG8KQ_000237"
     CDS             complement(39055..40041)
                     /locus_tag="ACG8KQ_000237"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9103213.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(40064..40630)
                     /locus_tag="ACG8KQ_000238"
     CDS             complement(40064..40630)
                     /locus_tag="ACG8KQ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP9103214.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(40644..41060)
                     /locus_tag="ACG8KQ_000239"
     CDS             complement(40644..41060)
                     /locus_tag="ACG8KQ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9103215.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(41517..43451)
                     /locus_tag="ACG8KQ_000240"
     CDS             complement(41517..43451)
                     /locus_tag="ACG8KQ_000240"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9103216.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            43753..44688
                     /gene="pplA"
                     /locus_tag="ACG8KQ_000241"
     CDS             43753..44688
                     /gene="pplA"
                     /locus_tag="ACG8KQ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP9103217.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            44778..45881
                     /locus_tag="ACG8KQ_000242"
     CDS             44778..45881
                     /locus_tag="ACG8KQ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9103218.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            45894..46847
                     /gene="menA"
                     /locus_tag="ACG8KQ_000243"
     CDS             45894..46847
                     /gene="menA"
                     /locus_tag="ACG8KQ_000243"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9103219.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(47661..49007)
                     /gene="gor"
                     /locus_tag="ACG8KQ_000244"
     CDS             complement(47661..49007)
                     /gene="gor"
                     /locus_tag="ACG8KQ_000244"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9103220.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(49141..50394)
                     /locus_tag="ACG8KQ_000245"
     CDS             complement(49141..50394)
                     /locus_tag="ACG8KQ_000245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9103221.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(50400..51323)
                     /locus_tag="ACG8KQ_000246"
     CDS             complement(50400..51323)
                     /locus_tag="ACG8KQ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9103222.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(51544..54036)
                     /locus_tag="ACG8KQ_000247"
     CDS             complement(51544..54036)
                     /locus_tag="ACG8KQ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9103223.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(54043..54510)
                     /locus_tag="ACG8KQ_000248"
     CDS             complement(54043..54510)
                     /locus_tag="ACG8KQ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9103224.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        15942 a        11436 c         9840 g        17425 t
ORIGIN      
        1 tacatattaa taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta
       61 gaagtaaaat gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt
      121 atcttagttt gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt
      181 tggtgagtta taatataatc tctggtttct atgggtactt cctcctaata agatgaatta
      241 actttattat cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat
      301 tccttagagc taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa
      361 gaattaatag actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa
      421 aaaattatat ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt
      481 atcctcccta aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt
      541 ttccctttat cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc
      601 agcatctcac ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt
      661 aatttctttt ttctttttga taattcctga attgctagta aataaattag tatagtagtc
      721 gttttaattt gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat
      781 ccaggttcca tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg
      841 aatcagtaaa aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg
      901 gtctatcatc cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt
      961 caactaagtg ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt
     1021 aagcagaagg cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa
     1081 tcattttttt atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat
     1141 ccaatttttc aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt
     1201 tttccgctaa ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc
     1261 aagcatgagg atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc
     1321 catcacttgc tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg
     1381 taaaccaagc atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt
     1441 ttgaagtttt ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag
     1501 gtacgatact gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa
     1561 tcgaattagt aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac
     1621 tatttattga acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta
     1681 ctgctttcca ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg
     1741 tgagattaga aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc
     1801 tgctatgtta aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt
     1861 tgcagaaata aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg
     1921 tgtaattaac attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac
     1981 taaagttaat agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt
     2041 tgccattaat ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac
     2101 caacactaaa accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat
     2161 atttccaaat aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac
     2221 tcctagtgct aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact
     2281 attgcttttt ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat
     2341 aaaaaaatta atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat
     2401 agcatcaccc ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac
     2461 aatccctata actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc
     2521 attaataaaa ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg
     2581 ctttttttaa attttcttca ttaaatacag attctacact tccgtgagca attagatttc
     2641 gattcaaaag aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa
     2701 ttagcactgt ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac
     2761 taactaagtc tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa
     2821 gacatcttgc taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat
     2881 tagaatattt tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat
     2941 ttttaattct ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta
     3001 cttttattgg aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca
     3061 gttttttatt gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta
     3121 acattgcttt aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag
     3181 caccttcttg tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat
     3241 taagcttttt cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca
     3301 tatggtatac aaaaattata ttttagtcaa ccctaaattt tatagtttag atttttttct
     3361 gttgctgaag attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca
     3421 aaatatataa aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc
     3481 gtatggaaaa attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac
     3541 atttataaaa attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac
     3601 tccatttaac ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa
     3661 taggaaaaaa actatttttg atcattgaca attaaataca atttttgaat aacgatccct
     3721 gctattcgag ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat
     3781 ttctttttgt tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg
     3841 taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca
     3901 aaaatttcat aactactatt ttaggagatg attttataga atccagagtc tacctgaata
     3961 aaaaacaaac ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta
     4021 tatataattt cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta
     4081 aagactttat gaatacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat
     4141 actgccttac tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata
     4201 taaaactaat ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa
     4261 gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa
     4321 gaaaatagaa aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat
     4381 aacgtttaaa aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat
     4441 tagatcaaac aggcctccta gtgatataac cattagtaat acaaaagata tatttcggtt
     4501 gcatatttta cctatgtttg gtgaatacgc aatgaattat ctaaatatca atactgagat
     4561 aatatcagat gaattaacca aaaaatcgaa agaatatgca aatataaaaa tcataaaagg
     4621 atatgttcgt tccatgttcg atattgcaga aatactaaat tatatcgaat ttaatcgaac
     4681 tactaaaatt attcaaagta tcactgcacc taaaaaaaat gctttagaag aaaaaagaat
     4741 acaagaaggt aagcaagcac tatcatcaaa agaacttact aattggatag aagcagtaaa
     4801 tgatgatttt aataatcatt tacttacttt tcatgattac acacttttta tgattaccct
     4861 gtatcttggt gatagaaaaa gtgagactta cgctttgcaa tggaaatata ttgattttga
     4921 aaaacaaacg gtccgtctta aacatacttt agataaatat caaagaaaaa agtttacaaa
     4981 aggtcgaaaa gataccgtca ttcaagtacc agaagttgta atgacattgc taagtgaatg
     5041 gaaatcggta caagctgatc aattgttgaa attaaaaatt aagcaaacgt tagatcagta
     5101 tctctttact tatactaaac catctggaga agtaaattgt cctgttcatg ccgattacct
     5161 aaactatcga atcaattcaa ttaaaagaag gcatcctgac ttagttcatt tatctccaca
     5221 caaactccgt catacttatg caactatagc tagacaaggt ggagcagata tgaatcaaat
     5281 ttcaaatgct ttaacacact cagatatttc tacaacaaag atatatgtga acacaccaga
     5341 tatagttgat aaggctgttt ttgaagcttt tcagagagga ttgaataagt gtgattgata
     5401 tgtgttctta aatgtgttct taaaaacaaa agaagcactt tccaaataat tgaaaagtgc
     5461 cagaaacgtt gatatataaa ggatattaac gttttgagaa ttgtgaagct ttacgtgctt
     5521 tcttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
     5581 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
     5641 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
     5701 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
     5761 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
     5821 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
     5881 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
     5941 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
     6001 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
     6061 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
     6121 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
     6181 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
     6241 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
     6301 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
     6361 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
     6421 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
     6481 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
     6541 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
     6601 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
     6661 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
     6721 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
     6781 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
     6841 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
     6901 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
     6961 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
     7021 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
     7081 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
     7141 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
     7201 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
     7261 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
     7321 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
     7381 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
     7441 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
     7501 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
     7561 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
     7621 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
     7681 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
     7741 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     7801 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     7861 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     7921 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     7981 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     8041 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     8101 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     8161 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     8221 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     8281 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     8341 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     8401 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     8461 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     8521 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     8581 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     8641 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     8701 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     8761 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     8821 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     8881 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     8941 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     9001 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     9061 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     9121 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     9181 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     9241 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     9301 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     9361 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     9421 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     9481 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     9541 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     9601 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     9661 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     9721 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     9781 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     9841 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     9901 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     9961 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
    10021 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
    10081 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
    10141 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
    10201 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
    10261 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
    10321 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
    10381 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
    10441 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
    10501 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
    10561 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
    10621 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
    10681 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
    10741 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
    10801 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
    10861 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
    10921 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
    10981 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
    11041 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
    11101 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
    11161 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
    11221 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
    11281 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
    11341 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
    11401 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
    11461 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
    11521 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
    11581 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
    11641 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
    11701 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
    11761 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
    11821 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
    11881 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
    11941 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
    12001 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
    12061 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
    12121 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
    12181 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
    12241 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
    12301 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
    12361 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
    12421 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
    12481 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
    12541 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
    12601 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
    12661 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
    12721 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
    12781 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
    12841 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
    12901 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
    12961 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
    13021 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
    13081 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
    13141 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
    13201 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
    13261 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
    13321 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
    13381 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
    13441 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
    13501 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
    13561 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
    13621 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
    13681 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
    13741 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
    13801 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
    13861 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
    13921 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
    13981 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
    14041 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
    14101 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
    14161 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
    14221 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
    14281 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
    14341 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
    14401 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
    14461 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
    14521 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
    14581 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
    14641 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
    14701 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
    14761 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
    14821 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
    14881 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
    14941 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
    15001 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
    15061 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
    15121 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
    15181 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
    15241 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
    15301 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
    15361 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
    15421 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
    15481 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
    15541 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
    15601 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
    15661 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
    15721 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
    15781 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
    15841 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
    15901 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
    15961 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
    16021 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
    16081 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
    16141 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
    16201 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
    16261 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
    16321 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
    16381 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
    16441 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
    16501 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
    16561 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
    16621 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
    16681 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
    16741 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    16801 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    16861 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    16921 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    16981 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    17041 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    17101 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    17161 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    17221 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    17281 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    17341 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    17401 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    17461 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    17521 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    17581 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    17641 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    17701 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    17761 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    17821 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    17881 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    17941 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    18001 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    18061 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    18121 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    18181 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    18241 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    18301 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    18361 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    18421 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    18481 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    18541 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    18601 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    18661 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    18721 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    18781 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    18841 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    18901 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    18961 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    19021 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    19081 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    19141 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    19201 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    19261 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    19321 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    19381 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    19441 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    19501 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    19561 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    19621 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    19681 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    19741 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    19801 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    19861 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    19921 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    19981 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    20041 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    20101 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    20161 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    20221 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    20281 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    20341 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    20401 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    20461 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    20521 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    20581 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    20641 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    20701 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    20761 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    20821 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    20881 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    20941 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    21001 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    21061 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    21121 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    21181 gtagtttact cgggtaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    21241 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    21301 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    21361 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    21421 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    21481 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    21541 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    21601 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    21661 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    21721 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    21781 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    21841 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    21901 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    21961 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    22021 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    22081 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    22141 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    22201 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    22261 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    22321 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    22381 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    22441 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    22501 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    22561 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    22621 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    22681 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    22741 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    22801 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    22861 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    22921 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    22981 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    23041 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    23101 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    23161 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    23221 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    23281 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    23341 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    23401 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    23461 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    23521 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    23581 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    23641 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    23701 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    23761 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    23821 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    23881 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    23941 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    24001 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    24061 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    24121 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    24181 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    24241 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    24301 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    24361 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    24421 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    24481 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    24541 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    24601 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    24661 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    24721 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    24781 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    24841 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    24901 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    24961 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    25021 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    25081 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    25141 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    25201 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    25261 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    25321 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    25381 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    25441 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    25501 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    25561 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    25621 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    25681 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    25741 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    25801 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    25861 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    25921 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    25981 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    26041 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    26101 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    26161 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    26221 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    26281 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    26341 ctagaaccat atcttgggaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    26401 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    26461 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    26521 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    26581 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    26641 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    26701 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    26761 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    26821 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    26881 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    26941 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    27001 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    27061 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    27121 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    27181 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    27241 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    27301 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    27361 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    27421 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    27481 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    27541 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    27601 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    27661 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    27721 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    27781 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    27841 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    27901 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    27961 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    28021 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    28081 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    28141 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    28201 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    28261 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    28321 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    28381 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    28441 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    28501 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    28561 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    28621 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    28681 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    28741 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    28801 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatcttca gcttcacgag
    28861 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    28921 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    28981 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    29041 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    29101 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    29161 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    29221 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    29281 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    29341 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    29401 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    29461 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    29521 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    29581 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    29641 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    29701 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    29761 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    29821 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    29881 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    29941 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    30001 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    30061 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    30121 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    30181 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    30241 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    30301 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    30361 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    30421 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    30481 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    30541 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    30601 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    30661 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    30721 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    30781 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    30841 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    30901 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    30961 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    31021 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    31081 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    31141 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    31201 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    31261 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    31321 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    31381 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    31441 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    31501 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    31561 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    31621 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    31681 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    31741 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    31801 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    31861 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    31921 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    31981 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    32041 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    32101 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    32161 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    32221 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    32281 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    32341 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    32401 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    32461 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    32521 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    32581 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    32641 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    32701 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    32761 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    32821 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    32881 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    32941 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    33001 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    33061 aaagcttggg taaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    33121 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    33181 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    33241 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    33301 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    33361 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    33421 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    33481 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    33541 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    33601 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    33661 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    33721 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    33781 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    33841 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    33901 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    33961 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    34021 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    34081 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    34141 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    34201 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    34261 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    34321 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    34381 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    34441 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    34501 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    34561 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    34621 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    34681 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    34741 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    34801 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    34861 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    34921 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    34981 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    35041 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    35101 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    35161 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    35221 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    35281 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    35341 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgtcaa actggcagca
    35401 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    35461 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    35521 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    35581 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    35641 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    35701 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    35761 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    35821 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    35881 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    35941 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    36001 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    36061 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    36121 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    36181 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    36241 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    36301 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    36361 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    36421 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    36481 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    36541 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    36601 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    36661 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    36721 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    36781 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    36841 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    36901 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    36961 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    37021 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    37081 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    37141 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    37201 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    37261 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    37321 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    37381 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    37441 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    37501 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    37561 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    37621 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    37681 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    37741 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    37801 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    37861 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    37921 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    37981 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    38041 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    38101 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    38161 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    38221 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    38281 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    38341 cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt gacagtacag
    38401 atactacctc taccacaagt gatagcacgg acacgtcagc ttcaagcgac agtacggata
    38461 catctaccac aacaagtgat agcacggaca cgtcagcttc aagtgacagt acagatacta
    38521 cctctaccac aagtgatagc tcagattcat ctaccactac aagtgatagc acggacacgt
    38581 cagcttcaag tgacagtaca gatacatcct ctaccacaag tgatagctca gattcatcaa
    38641 cttcaagcga tagtacagac acgtcgtcta ccacaagcga tacacgtcat gcaagtgtct
    38701 ccttctcaaa aacaagttcg gataacagga cgaactttaa cttaactaat aaaacaagca
    38761 atgacaatgg aaacaaatca aaaagagcac ttcctaaaac tggaagtcaa tcgaataatt
    38821 ggataacact cgcaggcgtt atattacttg taatcggttt aagaatcact tttatgagtt
    38881 ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa agagcaacgt cgctaacgga
    38941 cgttgctctt tatttttgct ttttatcata aatacaagaa ctacaattca atttgtcaaa
    39001 aaacacgtag atttccttct gagaaattta tttacgtatc gaacctttcc ctattcagtc
    39061 tttgcgatcc aaaatcagtt gggtaaggga aaagagattc tcttttgttc gaagagaggt
    39121 ggcaggtaat ttctttattt ccttcaaagc cttttctgtg taagaggccg ctaacttcct
    39181 agcttcttcc acgcctcctg tatgttcaac gattttgcgt acggcgtcga catcttcttc
    39241 tgtcattgcc gctttcttct ctaaataagg tagtagcttt ggtttgtttt tttgcaaaga
    39301 gtagatcaac ggtaaagaat agaccccttg acgcatgtct tctagtacag gtttgccgat
    39361 ttcctggctc tcttttgtat aatctaaaat atcatcgata atctgaaagg ccatcccgat
    39421 agaaagtccg atatctcctg ctttttttgc gaagcgctcg cttgttccac tttcaaaagc
    39481 gccaacagaa caacttaatt ggaacagttc agctgtctta cctgaaatat tttccaaata
    39541 ttcttttaca gaaagttcat aattatagcg atcatccatc tgccctaatt cgccattcaa
    39601 tactttttcc atactgcgag aattcaattg gagacttttc aaggaagtgg agtaatcaga
    39661 caatagttta aaacagcaga caaataaata gtctcctgca tagacagaaa cgacattacc
    39721 aaactgacta cggatattag gctgaccacg tcgcagatcc gcttcatcaa cgatatcatc
    39781 atggatcagt gtggccgtat ggagcatttc gatagaagct gccaaagcga tggctttgtc
    39841 tcgatcttgc tctggtccaa attgtgagaa caatagctga taggcaggac gcaacagctt
    39901 tccgccggca tggatcgttt ctttgattgc ttgttcgaca ggtttgtttt tcaggcggac
    39961 agtttcttcc atccgtttta gtgtttctgt caattcttta cttaaagcag gatagttttc
    40021 ccacattgga tgtagcttca taaaaaaact ccttgtgtga cgattatttt ttggtaaaca
    40081 ttgggctcca tttttgtttg gttccctgct cataatcttt ttggaatcgt tgaagtgtct
    40141 gcacatgtcg taaaagataa ttcgctgcga tcccgattgc aatccctgat aaaatcccta
    40201 aaactgaaag gactggcaag tacaacatga ctgtccatga ttgtgcaatg aaagaagcca
    40261 tcaaaagctg tccgacatta tgcatgaaac cgccggccgc actaattccg atgatactga
    40321 cacgttttgg tcctaattgc ttgaccaata acattccaaa ataactaagc aaagcaccac
    40381 tcatactata aaggaatgtg gaaatcgttc cgcctagcaa agtagtcagg aatagtcgta
    40441 gccaaagtag taagaagctg tctcgttttt tcatggtaaa aatggcaacg atcgtgatta
    40501 gattggctaa gcctagtttt gcaccaggcg caaaagaaaa aggataaggg atcatatttt
    40561 ccaataaacc aatgatcact ccttgtgcaa caagtagcga aatataaatc attttttgta
    40621 atttactcat ggtgtgtgaa ggtctaataa atcagactgt cttcgtcacc tccatctgtt
    40681 gaacggactt cgatcactaa tttatgtggc aaacagacga tggtttcgcc atttttcgtt
    40741 gcccagcctc gacgtacgca gatctggtca tcgcaatctg cttcttttat ccgtactcga
    40801 tcgccatcta tctcgatcaa gttataatct ccgtgttcat ctttgtaagt atatgtgttg
    40861 ctctcttctc cagccatcaa agggaatgtt ttgatttctg tgccatccac acgcagtacc
    40921 gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa aaatcatgac tggtaaaaaa
    40981 gaaagaacag tcaaaagcaa aataataaaa acatcccaag gtttgagcag acttttttca
    41041 gcaaattctt taaggctcat ttttgttctc ctttttccag attaactcac ccaattttag
    41101 cataaaaaca agaggttgtg acacctgtgt cttgctttga gtaataagaa cgaagaatag
    41161 aaaacagctt tccatgtttt ctaattcttt gggcttatta ccgagaagca gcaggtggaa
    41221 caccgtttat aacaagaggt tgtgacagaa gtgggcaact tcaagaaata agaaggaatt
    41281 tacgaaaatt acttttcaag tttttgtgaa atttcggctt atttttcgaa gaagctactt
    41341 ctggaacacc gtttataaca agaggttgta acagaggttg tgacacctgt gtcttgctca
    41401 tggaggctta tctcgggcat cttattcctt taattttaca taaaaaggag gctgtgacgg
    41461 tttgtctcag cccctttcta ttgaaatctt gcattcaaat aacgttcttt atctatttat
    41521 ttcttgccgt actgggaact tggtctaccg taccaccaat tacctagtgt acgttgtagc
    41581 catggaatta cccagcgatc caacccaatc gcacgtccgg aaccgttcat caatgcgaaa
    41641 gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc ctgataagca gaacgtgatc
    41701 accaatacaa tcgttgtcgc actgcatagc catgtgaata atccagcaat caatgctaat
    41761 gccaaggcaa tttcaaagaa taccatgaat ttttggaaga acattgctac atcattgttt
    41821 ggcatgatca cttgcatgat ctttgtgaac cagtctggtg acttctcaat cacagccatt
    41881 ggttcttcac cgtatgcata actcaaacca aaatgtgcag cttgagtcgc tgcttctccg
    41941 gcacttgccc ctgctccgct ggcagctgtg gttgcatcac cagcagcaga agcaccactg
    42001 gcaacatctg ttacattgta ttcccatgtc caagggaaca cactgtttcc agtgaaccat
    42061 gatccttcac caaaccaagt acttggtttg aacacttcac cattcccaat gatttttttg
    42121 gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa gtggcacact ccataagaca
    42181 ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat catctttgat atggaaaaac
    42241 tcatgcatta aatattggaa catataataa ccagaacgaa tatcaaagaa gtatttcaaa
    42301 ttcacgatat gtttcattac gatagctaaa aatccgctta aatggatctt atcaaatagg
    42361 ttagctaccc cccatttggc accaatagaa accataaatc cttgatagtt tcctttaaat
    42421 ttatgctttt ctccgccttt gatcgaagcc acgatattcg ctgcggctgt gtgtcctgtt
    42481 tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg ggaattcttc ataaaagact
    42541 aaatcaccaa tgatatagat gtttttatct tcgtagcctt ttgcctgcat atattcattg
    42601 gcgatcaacc gctgtccgcg tgctgattct aatccaaagt cagcagcgtc agaagttcct
    42661 ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt tgcgtccgtc tttcaatttg
    42721 atatgatcag cagccacttc tacgattggt gcattcaata ataattccac gttgtgctta
    42781 cgcaaatagc gttcagcttt tgcagcatca tttcgatcca acatattcaa gatcgtcggc
    42841 attgcctcta cgaccatcag tgtgatttcg tttggatcta atttataatc tttggctagg
    42901 cgatttttcc agtcgatcag ttccccaatc atttcaatac cggtaaatcc agaaccacag
    42961 acaacgaaag tcagcatggc tttacgcagt tcatcatctg gttccaatgc agcttttgct
    43021 actgtcttct caatatgttc ccgaatcctt acagaatctt caaatgacca aagggtgaat
    43081 ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct ctccgcccat tccaaggatc
    43141 agctgatcaa aaggatagga acctaatttg gtcttgacga tttttcgttc tttatcgata
    43201 ccgattacag tgtctgtgac aagttgtaca ttcttttgac gagcaaacaa atgctgcaga
    43261 tcgtattgga ttgcttctgg ttctacacgt ccgccagcta cttcatgcaa ctcggtcatc
    43321 atagtatggt acgaatgacg atcgatcaga gtaattgcta catcttcttt tttcagtttc
    43381 tttgccaagt attttgtagc tgaaacaccg gcatatcctg ctcctacaac gacaatattt
    43441 tgctttgcca ttgatcatcc gctccttata gactatctat ataatgaata tatgataatt
    43501 cttttagttt gatacttatt acacaatctc catatattat atagatgttc tgttcttttg
    43561 tctaactcct tttggatttt tttcaaattt taaaataaag cgttttttca cgaaatttgc
    43621 tgaaagaaaa cgtttgaaat attacatggg ttatttatca aaaatagttg acttataggc
    43681 taaaaagatt agaatgtata atgaatcaca aagaaaaatt gtggactaga aatggaatgg
    43741 tggggtatta aaatgaagaa aaaacgtttt gtaactggat ttgccgtatt agcattttca
    43801 gctctagtgt taggcgcttg cggcgcagat gacaacggaa gctcaaactc ttcttctgaa
    43861 tcatctacag cacaatcttc gactgcaaaa agcacaacag aatcatctgc aaaagttgta
    43921 gctggcggag atttgcaaga cggcacatat aaattagaag aaaaaaatta ttcaaatggt
    43981 taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa tcactgaatc aaaatatgat
    44041 aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag aatacaacaa aaacatggaa
    44101 gcaaaatctg gcacaaaccc agaaaaatac attcctgagt tgaatgacca attcttaaaa
    44161 gcacaaagcg caagcggtgt ggaagtcgta actggagcta ctcactcatc tgaaagcttc
    44221 caaaactacg cacaacaatt gatccaagct gctcaagctg gtaacacaga tacaatcgaa
    44281 atcgacaatg gtgctgattt gaaagacggc acttacaagc ttgaagaaaa gaactactct
    44341 aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg gcaaagtaac tgaatcaaac
    44401 tttgattata tcgacaaaga cggtaaatca aaacaagatg atacagaata taatgaaaat
    44461 atgaaagcaa aaagcggtac agaaccaaaa acttacatcc ctactctgaa cgacgaattc
    44521 gtcaaagcaa tgggcgaaga agatggctca cctgctaatg tagaagttgt tacaggtgct
    44581 acacacagct cacattcatt catcatgtac gcacaacaat tagtgaatgc agctgaaaaa
    44641 ggcgacactc aaacaatcga agtagataat atcgtaacag aaaaataatc acataatcat
    44701 actaataaaa gttggagggt gggacgatag ccagattagg cagtcgtctc acctttcaaa
    44761 atgaagagag gaaaaagatg cggaaaaaac tactattttc cttaatcaca ctgggcatct
    44821 cttttatggt gctaactggc tgcagcaact cagcaaataa aacaaccact gaaagttcag
    44881 cagctaccaa gatattgaaa gatccatatt ctgatgaaca gttcttgtta gggacttacg
    44941 ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt gaaacctgcc ttcgatcgag
    45001 taaaagaact tggtgataaa atcacgatca atcaaaaagg ttctgagatc gatgaagtga
    45061 atgagcaagc tgggatcaaa ccagtaaaag tttctgatga cgtctacacg ttagtcaagc
    45121 gggcctacga atatagccaa gattctcaag gcggattcga tatggcgatt ggtgcaatca
    45181 cacaattatg gcgaatcggt tttgatgacg ccagaaaacc ttcccaagaa gaaatcgatc
    45241 aggcattgaa attagtggat tatcataaga tcgaactaaa tgacaaagaa aaaactgttt
    45301 atctaaaaga aaaaggaatg atcatcgatt taggtgcaat cgccaaaggt tacattacgg
    45361 acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac tgccatcgtt gacttgggcg
    45421 gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa ccaagactgg acagtcggca
    45481 tccaagatcc gaacatggct cgcggatctg ttttaggttc tattaaagaa cggaacaaaa
    45541 cattagttac ctctggaatt tacgagcgtt atttagaagt agatggcaaa aaataccatc
    45601 atttgtttga tccaaaaacg ggttatccgt tcgacaatga tatcgcaagt gtcacgatca
    45661 taacagataa atcaattgat ggtgacgggt tgtctacagc agtcttttca atgggtgtga
    45721 aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg gacggaagcg atcttcgtga
    45781 caaaagacga taaagtatat gtgaccgatc caatcaaaga caccttcaaa ttatcggagg
    45841 actcccatta cacgatgggt gaccgttctg aactaaaata aggagtacga accatgacac
    45901 taaaaatctt tttagaagta gttgaaatac gaacaaaggt ggctagtgta tttccttttg
    45961 cggtaggggt attattctct atcgcttttt ttcatgagat acattggcta aacacttttt
    46021 tattttttat tggaatgcta gtttttgatt tagcgactac cgctatcaat aattacatgg
    46081 attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt ggccgcagaa
    46141 atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt gtttttgtta
    46201 tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga tttatctgtt
    46261 gttttattgg agtattctat acattcggac cgattccttt atctcgaatg cctttagggg
    46321 aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt tatgtcaata
    46381 cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa tttgctttgg
    46441 taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg gtgtttacga
    46501 ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt gaaaatcatc
    46561 gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc cagctattga
    46621 tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat gaatggccga
    46681 tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa ttcaagcaaa
    46741 cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta ttcaatgcca
    46801 gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag catgaagagg
    46861 ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat agctccttat
    46921 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttatcagg
    46981 aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc
    47041 cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatca
    47101 gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa aaatagttcc
    47161 tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca ctatttgttc
    47221 ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca cttgatttga
    47281 ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg tattctttta
    47341 acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt aaacatctgt
    47401 tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac ttaagagtgc
    47461 ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat ctccaacttc
    47521 cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact aaaaaacaaa
    47581 aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa taaaaagctg
    47641 ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc tgttggatgg
    47701 atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc cacggcaaaa
    47761 ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat tctttcttct
    47821 tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa tgcaaagtac
    47881 attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt ttccaatgct
    47941 tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac tgtcggaaca
    48001 agtttatagt caagataaga atctttcttt ccattaaata aacgttctga taaacgtcta
    48061 ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc aacagcaaaa
    48121 atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt tttgtccaat
    48181 tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc accggcaaaa
    48241 agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata ttcgtcattg
    48301 tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc ttcttggtac
    48361 atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg ttcttttcga
    48421 tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc agccgcaatg
    48481 tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa aaatccattt
    48541 gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc tccagtagcg
    48601 atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg actatcgata
    48661 aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct ttgataagca
    48721 ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa atcaacattt
    48781 tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc caaaatcgta
    48841 cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt tccgcccaat
    48901 tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt tgctgaagca
    48961 ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt gttcctcctt
    49021 attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa aacaagtgta
    49081 acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc agctttttcc
    49141 ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg gcatagttaa
    49201 aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat gcataacttc
    49261 aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat ctcctacaga
    49321 aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag ataatacttc
    49381 accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt cgttttcagt
    49441 tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc ataactcgtc
    49501 gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt ctacagccgc
    49561 tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc cttcgatctt
    49621 gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg acatatccac
    49681 tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt ttcgttcttg
    49741 accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac atccgccacg
    49801 gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat aagagataca
    49861 catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa tctctgccac
    49921 ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt ctttccagaa
    49981 ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg gcagacctgg
    50041 ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg cagaaatacc
    50101 gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt catgggtgac
    50161 catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa ctccttcagc
    50221 catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat tcccgccaat
    50281 atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg ttcctgctgg
    50341 cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca tcatactcct
    50401 tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg gttttcagga
    50461 tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca gctaccttct
    50521 aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt ttcgcctgga
    50581 gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc attcaattca
    50641 ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc ttcataaata
    50701 gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc acgatctttt
    50761 tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt ggattgatgg
    50821 atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc ttccatcgcc
    50881 ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc ttttttcgcc
    50941 cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat atacggtata
    51001 cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac tgtgatcaca
    51061 tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat tttttcagga
    51121 tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc taattctacc
    51181 aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa ttgctcttca
    51241 ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc aatagtttct
    51301 actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat agtagcatag
    51361 ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa ttatttcaca
    51421 aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt gtgacaaaaa
    51481 tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac acaggccaag
    51541 taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg tgatcttacc
    51601 ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa gcaaagcctc
    51661 gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg caccatattc
    51721 aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt tcacttgaat
    51781 atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga ttgtatggat
    51841 ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca agaattcagg
    51901 tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt gatctttagt
    51961 aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg ctccgatatt
    52021 tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag aatctgttaa
    52081 atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg ctttctccac
    52141 ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca actgtccacc
    52201 ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac tgtatttttc
    52261 cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca tgacttctga
    52321 aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca tgaatgaacc
    52381 aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac gtgaaactgc
    52441 tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca gttctaacaa
    52501 gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact ccgatacaac
    52561 tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt ttgcttcaat
    52621 tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta attgagcagc
    52681 attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt catcgatttc
    52741 tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga ctttcgctgc
    52801 tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga tataacgaac
    52861 agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat ggtgctcttc
    52921 atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg gctcatctac
    52981 ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt tttgatattc
    53041 atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg gtttcaagat
    53101 attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat gcaattcatc
    53161 aatgaataag attacttgac catcattata gatttcgtcg atgacctttt tcattcgatc
    53221 ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa gcatcatcag
    53281 gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag ctaacccttc
    53341 agcaatggct gttttgccta caccaggttc acccaccagt acagggttat tctttgtccg
    53401 tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa ctgggtccag
    53461 cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg taggtgttcc
    53521 ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca ttccttttcc
    53581 agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt ttgaaaggct
    53641 gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta ataagctcat
    53701 caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc tgctagctaa
    53761 agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag ggtaagaacg
    53821 gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt cattcaaatc
    53881 catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg ctagtaaaac
    53941 atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg caatttctaa
    54001 tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc catcttcata
    54061 acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt catattcatt
    54121 gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt tttttgtaat
    54181 tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac tgattgtatc
    54241 atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc ttacttttgc
    54301 aattcgtata taaccaccac caccacgttt gctttctact agataaccac gttggatggt
    54361 aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga ggtctgccat
    54421 ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta tatatgattc
    54481 aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact aatattgacc
    54541 ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta aataaatcct
    54601 ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DAZKCE010000005        50981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000005 DAZKCE010000000
VERSION     DAZKCE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.005"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..891
                     /locus_tag="ACG8KQ_000249"
     CDS             46..891
                     /locus_tag="ACG8KQ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9103225.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1172..2392
                     /locus_tag="ACG8KQ_000250"
     CDS             1172..2392
                     /locus_tag="ACG8KQ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9103226.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2389..5109
                     /locus_tag="ACG8KQ_000251"
     CDS             2389..5109
                     /locus_tag="ACG8KQ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9103227.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5093..6037
                     /locus_tag="ACG8KQ_000252"
     CDS             5093..6037
                     /locus_tag="ACG8KQ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9103228.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6106..7116)
                     /locus_tag="ACG8KQ_000253"
     CDS             complement(6106..7116)
                     /locus_tag="ACG8KQ_000253"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9103229.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7296..7640)
                     /locus_tag="ACG8KQ_000254"
     CDS             complement(7296..7640)
                     /locus_tag="ACG8KQ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9103230.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7633..8067)
                     /locus_tag="ACG8KQ_000255"
     CDS             complement(7633..8067)
                     /locus_tag="ACG8KQ_000255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9103231.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8205..8942
                     /locus_tag="ACG8KQ_000256"
     CDS             8205..8942
                     /locus_tag="ACG8KQ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103232.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8935..10146
                     /locus_tag="ACG8KQ_000257"
     CDS             8935..10146
                     /locus_tag="ACG8KQ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103233.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10350..11132
                     /locus_tag="ACG8KQ_000258"
     CDS             10350..11132
                     /locus_tag="ACG8KQ_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9103234.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11202..11864
                     /gene="trmB"
                     /locus_tag="ACG8KQ_000259"
     CDS             11202..11864
                     /gene="trmB"
                     /locus_tag="ACG8KQ_000259"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9103235.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11962..13716)
                     /gene="ade"
                     /locus_tag="ACG8KQ_000260"
     CDS             complement(11962..13716)
                     /gene="ade"
                     /locus_tag="ACG8KQ_000260"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9103236.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13848..14777
                     /gene="comGA"
                     /locus_tag="ACG8KQ_000261"
     CDS             13848..14777
                     /gene="comGA"
                     /locus_tag="ACG8KQ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9103237.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14734..15783
                     /gene="comGB"
                     /locus_tag="ACG8KQ_000262"
     CDS             14734..15783
                     /gene="comGB"
                     /locus_tag="ACG8KQ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9103238.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15780..16058
                     /gene="comGC"
                     /locus_tag="ACG8KQ_000263"
     CDS             15780..16058
                     /gene="comGC"
                     /locus_tag="ACG8KQ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9103239.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16033..16503
                     /gene="comGD"
                     /locus_tag="ACG8KQ_000264"
     CDS             16033..16503
                     /gene="comGD"
                     /locus_tag="ACG8KQ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9103240.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16475..16822
                     /locus_tag="ACG8KQ_000265"
     CDS             16475..16822
                     /locus_tag="ACG8KQ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103241.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16785..17210
                     /gene="comGF"
                     /locus_tag="ACG8KQ_000266"
     CDS             16785..17210
                     /gene="comGF"
                     /locus_tag="ACG8KQ_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9103242.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17182..17541
                     /gene="comGG"
                     /locus_tag="ACG8KQ_000267"
     CDS             17182..17541
                     /gene="comGG"
                     /locus_tag="ACG8KQ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9103243.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17713..18720
                     /locus_tag="ACG8KQ_000268"
     CDS             17713..18720
                     /locus_tag="ACG8KQ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9103244.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18745..19929
                     /locus_tag="ACG8KQ_000269"
     CDS             18745..19929
                     /locus_tag="ACG8KQ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9103245.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20366..21949)
                     /gene="abc-f"
                     /locus_tag="ACG8KQ_000270"
     CDS             complement(20366..21949)
                     /gene="abc-f"
                     /locus_tag="ACG8KQ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9103246.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22054..22182)
                     /locus_tag="ACG8KQ_000271"
     CDS             complement(22054..22182)
                     /locus_tag="ACG8KQ_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103247.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22527..22670)
                     /locus_tag="ACG8KQ_000272"
     CDS             complement(22527..22670)
                     /locus_tag="ACG8KQ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9103248.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22893..23372)
                     /locus_tag="ACG8KQ_000273"
     CDS             complement(22893..23372)
                     /locus_tag="ACG8KQ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9103249.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23445..23729)
                     /locus_tag="ACG8KQ_000274"
     CDS             complement(23445..23729)
                     /locus_tag="ACG8KQ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103250.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23807..24001)
                     /gene="ssrS"
                     /locus_tag="ACG8KQ_000275"
     ncRNA           complement(23807..24001)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8KQ_000275"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24073..25359)
                     /locus_tag="ACG8KQ_000276"
     CDS             complement(24073..25359)
                     /locus_tag="ACG8KQ_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9103251.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25497..26168
                     /locus_tag="ACG8KQ_000277"
     CDS             25497..26168
                     /locus_tag="ACG8KQ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9103252.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26134..26622
                     /locus_tag="ACG8KQ_000278"
     CDS             26134..26622
                     /locus_tag="ACG8KQ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9103253.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26635..27582
                     /gene="prmA"
                     /locus_tag="ACG8KQ_000279"
     CDS             26635..27582
                     /gene="prmA"
                     /locus_tag="ACG8KQ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9103254.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27583..28347
                     /locus_tag="ACG8KQ_000280"
     CDS             27583..28347
                     /locus_tag="ACG8KQ_000280"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9103255.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28580..30793
                     /locus_tag="ACG8KQ_000281"
     CDS             28580..30793
                     /locus_tag="ACG8KQ_000281"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9103256.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30804..31250
                     /gene="dtd"
                     /locus_tag="ACG8KQ_000282"
     CDS             30804..31250
                     /gene="dtd"
                     /locus_tag="ACG8KQ_000282"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9103257.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31421..32680
                     /locus_tag="ACG8KQ_000283"
     CDS             31421..32680
                     /locus_tag="ACG8KQ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9103258.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32769..33716)
                     /locus_tag="ACG8KQ_000284"
     CDS             complement(32769..33716)
                     /locus_tag="ACG8KQ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9103259.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34110..35402
                     /gene="hisS"
                     /locus_tag="ACG8KQ_000285"
     CDS             34110..35402
                     /gene="hisS"
                     /locus_tag="ACG8KQ_000285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9103260.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35417..37186
                     /gene="aspS"
                     /locus_tag="ACG8KQ_000286"
     CDS             35417..37186
                     /gene="aspS"
                     /locus_tag="ACG8KQ_000286"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9103261.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37295..38170
                     /locus_tag="ACG8KQ_000287"
     CDS             37295..38170
                     /locus_tag="ACG8KQ_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9103262.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38436..40319
                     /locus_tag="ACG8KQ_000288"
     CDS             38436..40319
                     /locus_tag="ACG8KQ_000288"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9103263.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40394..40678)
                     /locus_tag="ACG8KQ_000289"
     CDS             complement(40394..40678)
                     /locus_tag="ACG8KQ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9103264.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40668..41402)
                     /locus_tag="ACG8KQ_000290"
     CDS             complement(40668..41402)
                     /locus_tag="ACG8KQ_000290"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9103265.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41531..42154
                     /locus_tag="ACG8KQ_000291"
     CDS             41531..42154
                     /locus_tag="ACG8KQ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9103266.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42346..43467
                     /locus_tag="ACG8KQ_000292"
     CDS             42346..43467
                     /locus_tag="ACG8KQ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9103267.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43474..46602
                     /locus_tag="ACG8KQ_000293"
     CDS             43474..46602
                     /locus_tag="ACG8KQ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9103268.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46621..49815
                     /locus_tag="ACG8KQ_000294"
     CDS             46621..49815
                     /locus_tag="ACG8KQ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9103269.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49961..50668)
                     /locus_tag="ACG8KQ_000295"
     CDS             complement(49961..50668)
                     /locus_tag="ACG8KQ_000295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9103270.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16682 a         8927 c        10601 g        14771 t
ORIGIN      
        1 tagacttaca ataagcagtg aatttataat caacgtatag gagacatgga tgaaatgaaa
       61 ctaattggta tagatttaga cggtactctt ttgaattctg accagagcat tagtcaaaaa
      121 aatgcaagaa caatgaaaca tttaccggaa gactgttttc cattcatttg ttctggaaga
      181 gaagtagaag acattaataa tattctcaaa aaatctggtt taaggatacc agcagtaggg
      241 ttaaatggtt ctgttggatt tgatggagaa aaaaagatat ttgagtcttc cttcgataaa
      301 aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc caaccaaaat atatacaaat
      361 ctcggaagtt atgaatctca gaattataaa aataaaatga aaaaaatttt tcacgaggta
      421 gggagtgaat tttctatcaa agagctaaac tatgagcttg agtatgaaaa atctatttgt
      481 tcaatccctt ataataatat tgaggaaatt atagaaaagg atgctatcag catttacaaa
      541 ttttttattt ttattcctaa tcgtcaattg aaagctaata tatttaacca tttgaaaaag
      601 atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat
      661 gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa gaaacctttg
      721 agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc agatattagt
      781 tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt cactgtttca
      841 aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata aactaattaa
      901 ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc cagtacaaat
      961 agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat tgtgacaaaa
     1021 atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca tttatcgtaa
     1081 tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat tgaaaatggt
     1141 atgataaaag aaaaaaagga ggaaattttt aatggtgaaa tttatccatg cggcagattt
     1201 acatatggat cggtcatttg aaggactgac aactcttgat aaaactgtac aggaaaaact
     1261 attgaagacg aatctgaccg ttttatcaaa tatcattgaa caggcaataa taaataacgt
     1321 tgattttgtt ttacttgcag gagataattt tcatcaaaat cgtccttcat taaaaataca
     1381 gaaacatttt tcagaacaga tgaaacggtt agaaaagaac catatacctg tatttataat
     1441 attcggaaat catgatttct atcaaaaaga gcggtattgg ttctcctttc ctaaaaatgt
     1501 ccatttattt acatccgaaa cggtagagac aaaaaaaata acgataaaaa gtggagaaac
     1561 tgtctctctc agtggattta gttatcgtca accgtggatt caaaaagaca aagtgatgga
     1621 atttccttct agagaactca cagattacca tattgggttg taccacgggg aacctggcgt
     1681 ctctcaaaaa ggaaactatg ctcctttcca accctcaaag atgcaagaaa agggatacga
     1741 ttattgggct ttagggcata tccatgtgcc aacggtctta aatgaaaagc aaacaattgt
     1801 ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa gcttctacat ctattctact
     1861 agttgaatta agtaaaggaa gttgccaaat acatccgatt aaagtagcag aggtctattg
     1921 gaagacaaag gaaatatcat tgaggaaagc aagaacgaca aaagaggtca ttgttcacat
     1981 tcgtcagcag ctttcaaaag tagaggatgg atttagttta attgagataa aattaaaaga
     2041 ttatgaccat ctcaatacag atgttttgga acggatacaa tcaggagagc tattagacta
     2101 tttgttagag gaattctcag ataggataaa tgatttcttt atttggcgta tttctctcat
     2161 agagaatact ttttatacga agaccccact tgctgcttct gctaaattga tggaacagtt
     2221 atttcaatac tatcagacac ctcaagattt ccaacaaatt ttaaatgaag tatatagtca
     2281 tcaagaagcg gctagaatat tgagcgaact gccggaatat caagaagaaa cacttgaaaa
     2341 agccaagcag ctgcttaatc aagatttttt atttgaggag gatcaggaat gaagatttta
     2401 aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt ttattcaggc
     2461 aatcagctga tctatggggg aaacgaagca ggaaaatcaa cgatttatca atttattcaa
     2521 gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac accaaaagac
     2581 ggctcagcct atggcggcaa aatatggctt aaacatcctg tatatggtga atttgctgtc
     2641 gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga tcaagtggga
     2701 agtgatgagt tactagaaaa actgatcgca cctttgacga aagagttatt ccagcaggta
     2761 tttactttcc agcaagagca gttgatggaa ctagatcatt tgagagaaaa agagctacat
     2821 gatgcgctga tttctttagg catcacgggc agttctcaag tattccaaaa gcggcaagaa
     2881 tattatcaga aagcacaaca attattcaag aaaaaaggcc aaaaattact aatcaatcaa
     2941 aaattaaatg aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga acaggaagct
     3001 tattttggca agttgatcca acaagaacag gaatacgagt cgcaacagca gcagttattg
     3061 gcacaattaa aagaggcgaa caaacaattt tcattagcca gacagcaaga acttaatttt
     3121 tctttatatg aagaatggca atctctcaaa aaaaacagca ctacaggtct tcctgatgag
     3181 caacttcttt tagatctgga gtctttctcc aaacggtatc agcaaacaaa tgagcgtttg
     3241 gaagaattag aagcaacact ggaaaagcat agcgggatgg atcagcagtc agccagatat
     3301 tatttttatt tagagcagga gcaggaacta aaagatctcc agcaacttca atttacagcg
     3361 gaacaattaa acgaagaatg gcaaagatta caaattgaga ctagagaaac aaattcagaa
     3421 ttgaagattt tggaacaaag gtggcattgg cagaaaaatc cgttaccgga agaattttta
     3481 tatgaagaag aatggaagca tcttctggct gaagaaagaa ggattgctga tctagaaagc
     3541 cagatttctg tgcgtctcca aatagctaaa gaacagcaag agatgattga agcagaagtg
     3601 aatgacttgg aagagaccta tccagcatta ttacaaaatc agtcagagat agcaccagtc
     3661 tcttcatcta aaccgtatat gctgatggct ggcggagcaa taatcattgc aggtatattt
     3721 gtaccaatgc ccttgaaagt gatcctttta ctaattggtg caggcatgat tggctatttc
     3781 ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt tctctgagat aaaaggaatg
     3841 tggcaagaaa aactaggaca acttgatcta gccttagctc aattagctga agcagaagaa
     3901 aaacaaaaga gaactggaga agaaaaacag attcttcagc aagaagtata tcgttttgcg
     3961 gatcatcggc acttaggaaa gatggatacg ctcaaactca tgaaagaata tggacaggaa
     4021 gtcatagatt atcagaaatt atatggcgta tttgagcgga aacataaaag agaacaggaa
     4081 atacaagaaa aactacgaca aatcgaccaa aaatttgagt ttgttaaaga atggttaccg
     4141 atacaagata agacaatttc agaaaaaatg gtattgcttt cccgttttat acaagagatg
     4201 gaacagatga aatttgccag aagctatcag caaaatactt tattgaagca agaaatcaat
     4261 caattgaaaa agaaacaaaa agtatggata caagaataca gcgaacaatt gactcaagca
     4321 ggcttatctt atccttcgga agtacctgtc tttttacaaa aagccaaaca agccgaacag
     4381 cagatgaaaa gatacaatga attatcggaa atgcttactc cactatttcc tgaagagctg
     4441 acaaaagaga aattatctgt aagactgcgc caactgaaag aaaagcaatc ccaactccag
     4501 caagaagaaa aagaagcaag tgaaaatcgg caacgactcc aacttcaagt agaagagttg
     4561 caaagagacg gcacattgga cgaactttat caacaagcta gccggcaaaa agcaaaatta
     4621 caggaattac tggaggaatg gggagcactt gaaatcgccg ggacattctt aggtgatctt
     4681 tcaacagaga tgagtgaaag acaattaccc cagttgttaa gagcagcagg tgattattta
     4741 ggtattttaa caaaaggaaa ttatcagaag atattactga aaaatgatac actgacttta
     4801 acagacggat atacagaatt tcccatctat gagttatcta caggtacgaa agatcagctg
     4861 attatggcga tgagatttgc ttacctctca ttagaagcta accgttcgat atgcccgatc
     4921 atcattgatg atggctggct tcattatgat catcagcgaa agtatcaatt agctgaactt
     4981 ttagctaaat ttggagaaaa gtatcaaata atttgttttt cttctgataa agaaatggta
     5041 agctattacc atgagatgaa ccaaatcgta aaacaattag aaggagcaaa taatgaaaaa
     5101 aattcgtgat ttagtaatcg atgagcagtt tgaacaattt gtcctgatca aaagtgcaga
     5161 tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt actttccaag atacttcagg
     5221 tactatcgat ggtaaatact gggatgcttc ggaggaggaa atcaataaat ttacagccgg
     5281 cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc aagtaaagat
     5341 tcttcatatg cgcttaacaa gggtagatga gccaggagag cctagtttat acatggaaag
     5401 agcccctttg aagaaagaag aaatggaaga agaaatcaat caattgttgt ttgagatcac
     5461 caatgcgcat tggaatcgta ttgttcgcta tctacttaat aaatatcaaa aacaattttt
     5521 tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc agtggattag cttatcatac
     5581 tttgacaatg cttcacttag ggaaatcaat cgccgatgaa tatcctgagc tgaatcgagc
     5641 attgttgtat gctggtatca ttttgcatga tttaggaaag gtgattgaac tttccggtcc
     5701 tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt catctggtat tgattgacga
     5761 agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag atgttctcgt
     5821 attacgtcat atggttttag cacaccatgg cttattagaa tatggctcac ctgtacgtcc
     5881 aagaataatg gaagcagaaa tattacatca aatcgacaat atcgatgctt cgatgcagat
     5941 gatgttgact tcgattcgtc aaacagaacc aggaaaatac accgatcgga ttttcggtat
     6001 ggacaaccga agtttctatg taccaaaaga tttatagcaa aaatttattt ttttcattca
     6061 aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt ttcttttatt ttgatgaatc
     6121 agttgtttct tcagttgaag attcttttgt tgaatcagtt gattttgtgt cagaactctt
     6181 tgttgaagct gtagtttcac ttgagtcttt cgtacttgat gaagttgttg tgaatgcact
     6241 caacacattt tcaaaggcat catctttgat ttttacattt gcatctttta attcttcacc
     6301 gattacttta gttgtgaatg cattgtcact tagtttagtt tcagtagcga tatcttttag
     6361 ttgatctttg tatttatcca tgtcattgcc tttattttgg tttttcacca ttttgacaac
     6421 gtagtattct gtcgcataag aagtaggatt tgttgtagta ataacatcgg agatctcacc
     6481 gtctttcaat ttgaaagctg cttcttttac ttctgcagga atagttgttg tcgttgagtc
     6541 gaatttaact ttaccgccat cttcttttgt ctctgtatca gttgatttgt ctttggctaa
     6601 tttagagaaa tcatctccat catccgctga ttttttcaca tcttttgctt catcttcact
     6661 tgataatttg atgatttgtg cttctacttc tggatggaaa gatttccaag cagttttcaa
     6721 atcgtcatct gtaatatcta cgtgagcttt caagccagct tcaaaagcta aattgttacg
     6781 aatatattct ttataagtat ctttcgtata tccagcagct tctaattgtg attcaaaagt
     6841 atcacctaat gatttcgctt gtttgtcgta ttctgcatcc acttgcttat cggttacttt
     6901 atcgccatat ttgttattga atactttata aatgatcatc cgttgtacta aggattggtt
     6961 cgatgattca agttttgctt catcatagaa atcagataca gtgatcgttc cgcctttcat
     7021 cgttgcgata tctttattag tatcgcctga acaagctgct agagttaaaa cagccaacgc
     7081 actagtagct gccaaaataa ttgatttttt tttcattaaa atttgcactc ctgtaattag
     7141 ttattatttt tatatattaa ctataccata tcccatttcc tttaaaaacg ttgtcgtggt
     7201 agtttcaaac aaaattcaaa atttcatcac attcctagga aatacgcaaa cttttctcac
     7261 ttatatagtt aaatctgttg agaaaataat cattattata tttgttcagg atactctggc
     7321 agatcagctt ttagtttttc tacttgttca ttgatttgtg tgatacgtgg ttcggcttgg
     7381 aatttaaaat cttcgatatc ttttttgatt gcttcttgga aagcaggaat cacagattca
     7441 gctgtttctt ttgtttcgat caatgcgctt ttaaaatgat tcaggctgtt atttaaatca
     7501 tttgtcagag ttgttgcttc ttccaattct tcgatcagtt tctttcttgt ttcattcccg
     7561 ctcctgggcg ccaataacag acctccgaca gtgccagcag cagctccaaa taacagtcct
     7621 ttgaaaaagt ttttaatcat cgcaggacac ctgctttacg attgtttctt gaatagcttt
     7681 tagttcttcc gatccataac tatcttgatg attgacaaag tgaatagaaa aatcatcctc
     7741 tttgctgtag cgagggacta aatggatatg tgaatgaaaa acagattgat aggctagttc
     7801 acggttgtta ttcaagatat taagaccttt catttctgga aaagcttttt ccaatgcttg
     7861 agcaatctta ggcacacgcg aaaaaacctc tcctgcaagt tcgggatcat attcaaaaat
     7921 atctgctaca tgacgtttag gcacaactaa tgtgtgtcct ttcgttgctt gagagatgtc
     7981 taaaaaagca tagaccttgt cgtcttcata tattttatag cttgggattt cttgattgat
     8041 gattttgcaa aaaatacagt tttccatata aaacatcctt tctataaaga ataaatactt
     8101 cagcttaact ttaccataag ttaaagctat tgtaaggaaa aaaacaaaat accgtcattt
     8161 tcttcgtggt ataataaata aaaataaatg gcggaggaaa aagaatgagt ttaattatcg
     8221 atcatgtcac aggggggtac ggacatctcc ctgtgttaaa agatatcagt ttcgatgtac
     8281 agcctggaga gatagtaggg ttgattggac tgaacggagc agggaaaagt acgaccatca
     8341 agcacattat cggtctgctg gaagcttcaa aaggaacgat agaaatcaat catctatcct
     8401 taaagaaaaa tacggaagaa tatcgcaaac aaattgggta tattccagag actccttctt
     8461 tatatgaaga attgacattg agagaacata tcgaagtcac tgcgatggcc tacgatatac
     8521 ctatggatat agcaatgaaa agagcagaaa agttgttgga gacttttcgg ttatctaatc
     8581 gtcttgactg gttcccagct aatttttcaa agggaatgaa acagaaagtg atgatcttat
     8641 gcgccttttt gatcgagcct tctttatata tcatcgacga accattttta ggcttggatc
     8701 cactagctat caatgcattg cttgaattga tggtagaaat gaaaaaagaa ggagccggta
     8761 ttttgatgtc tacgcatatt ttagctactg cagaaaaata ttgtgatcgt tttgtggtct
     8821 tacataacgg agagatacga gcagacggtt ctcttgagat gttgagaaaa gaatttcagc
     8881 tgccggattc ttctttggac gaaatctata ttgctttaac aaaggagcag gcacatgagt
     8941 agtttttttc aaaaacgttt agcaagacat caaaagaaaa tgatgaaata tatgcgttat
     9001 gtattgaacg atcattttgt tttagtttgt cttttcttga ttggcggtgt gggattatat
     9061 tattctaatt ggttgaaaac cctgcagcca ccgtttgcaa ttggagggct cataatctct
     9121 gtattttgga tgctctgttt attcgtgggt aagatggcta cttttgcaga ggcagcagat
     9181 attgtttttt tacttcctaa agaaaaagaa atgcaaacct atttggaacg agcttttcgc
     9241 tattcttgca tttttccgtt gatctttttg atattggcat gcggattggc catgccatta
     9301 gtggtggtta gtacggagca atccttttcg atgtttttcg tctatctgat catattatgg
     9361 agtttgaaat tcagtcatct gcaaatcaag agagcagaat tgtttcgact agatcaacaa
     9421 gttattcgtt attggaaatt aggctggttt ttttcttctc tgatcttttt gtttctatca
     9481 ttttattggc taatatgggc aggaatagca ggtgcattag tacaggcaag tctatattac
     9541 tggtttttgt ggaagcaaca agaaaaagga ttggattggg agaaaatggt gcatgtagaa
     9601 gaacataggt tgcatcagat ctatcaattt atcaatttat tcacagatgt tcctgaaatt
     9661 acggcaaaga tcaaaagaag acgctatttg gatcctatat tgactcatat taaaagagaa
     9721 tcaaaaaaca cttatcttta cttgtttgca agaagattgg caagaggttc tgattttagt
     9781 ggtctttatt tccgattagt aattataggt gggttattga ttgccggagt agtcgacttt
     9841 tattttagtc tgattatcgg tttcttattt ttatatttga tcggtttcca actgttacct
     9901 ttagcgaacc agttccgcta tatggttctc gttcagcttt atccaatcca taagaatcag
     9961 aagaagcagg cgattcagta tctactcaca tgggtattaa ttatcgcgtc ggttattttt
    10021 ggtttgattg ctgcagtcgt attagatggg cagcagcgat gggttccgct agcggcttat
    10081 ttgattgtta ctatgctctt tgttaaacta tatgtaccaa ctcgtttgaa gaagatgtca
    10141 gactgaaaac aaagctgtgt agggtaaaaa tgagaatgaa aaatagagcg ttgacagtaa
    10201 gaatgctgtt cgttctattt tttttgagaa aagctggttt tttgaatggc aattaaatga
    10261 aaaagaacgt atatgtgctt gacgtgtagt gttcaggcga ctagaataat aagtactgca
    10321 aaacaaagag taagaggacg ggagaatgca tgatggagtt tcagttagac caagagtggc
    10381 ggttacgtcc catcaaaggc gacacaggaa aaacatacat aggcatgcgt gatgaggaca
    10441 aagtctttat taaacgaaac acgactccga tgttggctgc tctttcaagg gaaggaatta
    10501 caccaaaact tgtttggaca aaacgaacgg ggaatggcga tactttgaca gcacaagagt
    10561 ggctggaagg tcgcttgctg actcctaatg agattggtca aagaaacgac gtgattgatg
    10621 ttttgtacca tttacatcat tcaaattctc ttaaaaccat gctgacccgt atcggtggtc
    10681 gtgtcatgac gccggagaag atgctgcaaa aatatgaaaa agaattaccg gaagaactcc
    10741 agaaaaatca atttctgcaa attgtccatc gttttcttcg tgagcagatc ccgtcaatag
    10801 ataaggaaaa cttatcagta gtacacggag atgtaaacca tcgtaattgg ttagtatgta
    10861 aaaattattt atatctagtc gattgggatt caatcatgtt tgcagaccct gcggtggata
    10921 ttgcaacgat tttaggaaat tatgtgccac tgtctagctg gaatcagtgg ctgatcagtt
    10981 atggcatacg cccgactgac gaaatgcttg aaaaatttca ttggtatgcg gccatgaatt
    11041 tgctccaaga aatcaccaga tattgcaaac gtgaagatca tcgacggatg aacgaagaaa
    11101 ttttacaatt aaaacgaatt ttcagtggct gaggtttggc ccaaactctg cggagatttg
    11161 gccagacctt tttaattaga ggaaaggaat ggagataaat aatgcgtgtc cgaaatcgtc
    11221 cgggagcagc ggaaatgtta gcggctcacc cgaattttgt aattagtgat ccaacattgt
    11281 ggaagggaaa atggaatgaa ttgtttgaaa acgatcatcc aatccacatt gagattggta
    11341 tggggaaagg ccagttcatc actggaatgg caaaagccca tcctgaaatc aattatattg
    11401 gagtagaaat gcaggtcagt gttgtttcga ttgcactaga caagttgatc gaacaaccgc
    11461 ttcctaacct gaaacttcta catgtggatg gatcagcgtt gacagagtat ttcgctgaca
    11521 gcgaagtaga tcaaatctat ctgaattttt ccgatccatg gccaaaaaaa cgtcatgaaa
    11581 aacggcgatt gacatataag acttttcttg ctgtagatga acagattctt cgtccaaatg
    11641 gagaaatcca tttcaaaaca gataatcaag gattgtttga gtattctctg gcaagttttt
    11701 ctcaatatgg catgatcctc aaacaggtat ggttagattt acatcaaagt caatttgaag
    11761 gcaatattat gacggagtat gaagaaaaat tttcttcaaa aggtcaacgt atttatcgag
    11821 tagaagctca atttcaagat aagaccaaaa agaaaagtct gtgaaactaa ctttctgctt
    11881 tcataaaaac attaatatcc ataaataaat cccagaaaat gaacattaca gatgtaatgt
    11941 attttctggg atttatttga tttaaaatgt atttatttca tcggtttttc cacaaggtgg
    12001 aatcaattgg aagcgagaaa atgaaaataa tcctttgtcg gtgatcttca attcgggtat
    12061 gacagataat gttaagaaag aaagtgtcaa aaaaggatca aaggttttag ctgctcctag
    12121 ttgatgtgct ttagttatta attcattcaa ctgttcatag acgatcgaaa aaggttcctg
    12181 tgtcatgatt ccgcctatag gaagagaaac acaagctatt tcctgctgtt tgttgacaac
    12241 ggtcatcccg ccaccttttt gaatcaaaag attagcagca tacagcatat cctcgtcgtt
    12301 tgtcccaacg acaactagat tatgcgagtc atgggcaatc gttgtcgcta tcgctccttg
    12361 tttcagctga aatcctttca ctatgcctaa tccaatgttt cccgtttgat ggtgacgctc
    12421 gatcaccgca atctttaatt ggtctttttc gattgaagga atgaatcgtt cgccttgtcg
    12481 gtccactttt tctaccaaat ctttagtcac tagactattc gggataattt cgatcacatg
    12541 ggcttgtgaa gaagaaagaa gcagctctaa cgaatgtgga tttaatggtt gagcgtttag
    12601 ttttggcaga caacacgtat atgaatttga atgatcagca aataaatggt cttggagttg
    12661 cccagattga acaactaatg ttcccttata gaaaacttca tgaattggca gcttggtaag
    12721 atcgtctgtt aagaagaaat cagcttgata cccaggggca atcgcaccta gtgttttcaa
    12781 atcaaaacat tctgctgcat tcaatgttgc catctgaatg gcttgtaatg gatcgattcc
    12841 aagtgcaata gcttgacgga cgatatgatc gattgatcct tcttttacta aatcattgat
    12901 cagtttatca tcggtgacaa agaagcatct tcttgaattt tcaggtgtga tcgccggtag
    12961 tagcgatgcc agatcttttg caacggtccc ttctcgaacc attaaataca taccaagatc
    13021 caaacgttct ttagcttctt ttgcagttgt agcttcatga tccgtccgaa tacctgctgc
    13081 aggataaaca tttaactctt ccatgccgat accagctgca tgcccgtcaa tttttttctt
    13141 atgctggtgc atcaattgga tcttttctag gatgtctgtt tcattatttg ctactgcttg
    13201 atagttcatg acttcagcta agcctaaaac ttcgggtttt tgtaaaaaag gggctaattt
    13261 ttttgcattt aaatttgcac cattggtctc aaaaggggtt acaggtacac aagaaggtag
    13321 cataaaaaag atattcatga gtgtttgtct tgcatcttct atcataaact caagacctgt
    13381 actcccagca atgtttccta tttcatgggg atctgtcact acagtagtaa ctccatgaag
    13441 caaggatact tttgaaaatt cgcttggtga taaaagtgag ctttcgatat gcacatggcc
    13501 atcgatgaat cctggcacta gatattgccc attagcatta tgtacgcttt ttccttcgta
    13561 ttcaccgatt cctacgatca ttccttcgca gattgctaca tctttttcca tgatttcttt
    13621 cgtaaagacg ttgacgatct tagcatgctt gatcactagg tcagcaggct tttttttact
    13681 tgctacttcg atatatttcc ttaatgttgt ttccaatgga gtgcctccca agataaattt
    13741 ttataagtat aacataaatt cggtttttgc caatattttt ttgtaaattc attttatttg
    13801 aaacaaaaag atcaaaaaga cgtatgtttt ttgtggaggg gagaataatg gaaataaaac
    13861 gattatcgga tcaaatgata agtgaaagtt ttgccaaaca tgcccaagac gtatatatat
    13921 tgccagcttc agaagtattt tacatctatt ttcgctatgg aaaaaaacga acattctgga
    13981 agaaagtctc ttcggcagat ggacagcaac tgatctcaag atttaagtat ttgggacaga
    14041 tggatatcgg agaaaaaaga aaagctcagt taggtgcatt tacttattct ttagaaaaac
    14101 aggaagttcg tctgcggttg tcttctgtag gagattacct tcaacgagag agtttagtga
    14161 ttcggctatt atacggtact tttgaaacac cccatcgttg ctttagttct cgagattttg
    14221 caactatttt acagaaaacc gtcagacgtg gattatacct tttcagcgga ccagtcggat
    14281 caggcaagac ttctctaatg tatcggttgg ctttggaaga aaaattgcaa gtgattacta
    14341 tcgaagatcc tgtagagata gaagaaccgt tttttctgca gctacagatc aatccgaaaa
    14401 tagcacagac ttatgataca cttttgagac tcgcattacg gcatcgtcca gacttgctaa
    14461 tcattggcga gataagagat gaatcaactg cacatgctgc ggtaagagca gcactaacag
    14521 ggcaccgggt ttttgcaacc gtacatgcac gcaatcttga aggtacattg gtcagaatcc
    14581 atgaactgac aaagaaaaaa gaagagctat ctgaatgttt agctggtgtc atttatcaag
    14641 aacttttgga agatgccaat caccatcctt ctgttttatg gagttatcaa tttatggagg
    14701 aagaatcagt aaaaaaatca tgggaggaaa gttatgaaga agctcaggaa aatatttggc
    14761 agtcagaact attctaaagg attaacaaga agccagcaaa accagtttgc acaaaccatg
    14821 ggagaactgc ttttatctgg ttttagtctg caagaatccc tttatttatt actcacgatc
    14881 cgttcgtttc cacctgttat tttggcaaat atccaacatc agctaaagga agggcaagcg
    14941 tttagtgagg tactttcgca aataggatat ggagcagagc aactggtaca gatcgagtta
    15001 gctgaacgtc atgggaatct gccgacaact ttgttggaaa ttgccaaaca gtttcggctt
    15061 gttgaaggat ttcgcaatga tttgaaaaag ctgcttgcat atccgatgct attgctcacc
    15121 tttttgctag gtatcttgtt tagtatgcgg ttgttcgttt tgcctcagct tattggttca
    15181 ggaatggtca tgcaagagca ttggggtatt cagctgatac aagtctttca ttggtatgtt
    15241 ctactattca tcctagcggt tactctattg gctttactag taaggtatcg tctgatgaag
    15301 ttgtctcctg ttgatcagag tgaatggata agcagacaat tattgattgg accgatatat
    15361 tccttgtatc aatcggctta tttgtcacta gagattggaa agctttttca tgaaggtttc
    15421 gaattaaggc aaatcatagt ctgcttaaag gaaacgaaac aagaaagtct gattcagcat
    15481 cttgcctcta agttgattca aggattggag gcaggtatgt cattggctga gcagttccag
    15541 aattatcgtt ttctatcaga agaattcagt aaaattgttt tacagggaga ggcaaaggga
    15601 agtttaggta aagaactgat tttttacagc gaatggacaa gaaaacagct tttcaaacgt
    15661 cttcatcact ggatgcattt tattcagcca atcatttttt tgggcgtagc ggctttgatt
    15721 ttattagttt atgccgctgt tttattacct gtttacggaa gcatagagga ggttttgcca
    15781 tgaaaaagag aaaaatatct tattatagcg gttttacatt gatcgaaatg ctaatcgtct
    15841 tactgatcat cagtgtatta gtgttgttgt tcgtccctaa tttgtcgcgt tataggaatc
    15901 atgtagacca agaaagtcgt gaagcgatca tccagttggt agacacacaa aaagagctct
    15961 atgcgcttca aaataatgga agagttccta ctgtggagga attgttgaat gaaggctata
    16021 tcaaacgaga acatgcagaa atctatcagc ggccttgaag gtaaggtttt agcatcagga
    16081 tatacgttgt ttgaatcact cttactgatt ttattactta caacgatcct ttcttttcca
    16141 gttctcgcat tttctgcatg gaagaatgaa ttggcagtct atcaattttt cggccaattt
    16201 gaaaaacgaa tctattctac gcaaaaaatc gctattgtga atcagatcca aacaggcttt
    16261 tattggaata gtgaagagaa tcaaatcatt tttcatgttc cgaatccaga taaaataaat
    16321 tgggaagtat tagatattcc tgatgagatt accttgaaac gccatgcttc tattactttt
    16381 gctgctagaa ccgggaatga aagttcacta aaagcttatc aattttattg ggaagaaaaa
    16441 gagcggacga ttacgtatca attccagata gggagtggtc gctatacaaa aagaattgaa
    16501 taaaacagga tttgttttgt tggagtccct tacagcgctt atgatcgctt ctgtctgtgt
    16561 ctacttagtg agctttggcc agctgttttt gcttcaggca gaaaagaaaa gtggggagga
    16621 actgctattt attcgaatga tttatgaaga tatcaagacg cagcggatgt atcacaaaag
    16681 accagagcga agggaagaaa tgatcccata cgatatccaa attgtcgata ataatggagt
    16741 aatcgaaaaa gtgtttgtca atatggagaa agaagcgatc agtattgaaa aaacaagtaa
    16801 ttccagcatt tacgctgatt gaatgtctaa ttggactgat gatattgagc agtctattgc
    16861 tcacattcaa tcttttgatt cagcaaacca tccaagtaag tgaatttttg cagcgtaaag
    16921 atcaaaaaga gtggctaata tttttagcac aattagaaga agaactcaag agcagtcaca
    16981 atgtctctgt gaaaaatcag caactgcact acacgatcga ggataaacag tatatcatcg
    17041 aaagatatca ggcaatgata cgtaaaagaa gaacttccgg cggacatcaa cctatgttaa
    17101 caagtatcac tgagcttcaa ttattggaaa aagaacacac aatttctttt tatgtacact
    17161 ttgaaaatgg agaagatgga tatgccatat ggacaaaaaa cgatcagtag aaaatgttat
    17221 aagggctcta ttttattttc tgtcttgctt ttatttcttt tatgtacgtt tttattgatg
    17281 gcagcattag aaagctaccg tttgtcaatg gatcttaata tacgaatcaa aaattattat
    17341 atagcaaaaa taatgaagca gatggcttta tcggaacagt ctacgcttgc cgaaagttcg
    17401 gaaggagttt tttactacac aactggatct gtaacatatc agtgggtaca acagtcccta
    17461 ttcttagagg tggaagtctc accgtttatc tttcggttca cagaagaagt aaaaaatgat
    17521 acagatacta gtacagaatg atggaatgtt cgtgaaaacg ccctcttttc tgttataaca
    17581 ctgtttttaa taaaaaaatg tttgccgttt cacaaataaa aaacagtgaa aacgattgaa
    17641 attttgtcaa ttgttcgata gaattaaaca ggtgaaaaaa ttgttataat atatctagaa
    17701 atgaggtgca ccatgttacc agaaaaaatg gaacaagggt ttaatcaaaa cctggaagct
    17761 attcagcttt tacagaatgc attgggaact tcctttttag aagcctatgt cgaaaatgca
    17821 gaaaatttaa tagatgatta tcaagtacgt gtggtggatg gagtgccgac aaaagagaca
    17881 acgcaacgga tcacagctct ttatgaagag ttgaagaaac tttctttcga gccagaagaa
    17941 tggcgcagac tgtctcaatt acttttattg aagggcagcc agacagaaca tttgcaggcg
    18001 aaccatcaac tcactcctga tagtatcggt tttttatttg tttttcttat tgaacaacta
    18061 tacacaaata aaaaagcacc ggttaagatt ctggatattg cggcaggaat gggcaacttg
    18121 ttattaacag ttcttttaaa cctatcgaat gcggggtacc aaacagaagg aattggtgtg
    18181 gatattgatg atacattgtt ggctgtcgca gcttctacaa gtgatttgac acaagcaaat
    18241 gttcaatact ttcatcaaga cgggttgcag gaattattaa tagatcctgt cgattttgca
    18301 atcagcgatt tgccaatcgg ttattatccg aatgacgaaa aagcaaaaga gtttttaaca
    18361 agtaccgaag aaggccatag ttatgcacat catttattgc tagagcaatc aatgaaatat
    18421 gtgaagccgg atggttttgg tttgtttttg atgccgagtg gatttttaga aacggatcaa
    18481 agtgaagaaa tcaaaaaatg gtttaaagaa gaaggttatc ttcaaggaat gattcaattg
    18541 ccagatgaat tgttccgtaa taaacaatcg caaaaaagta ttttgatctt acagaaaaaa
    18601 ggaccacaag cacaacaagt aaaagaagtc ttgcttgtga aacttgcttc attaaaagaa
    18661 ccagagaagg tgacggaatt tttcaacgag tttaaaaact ggaaatcatc tgctctgtaa
    18721 tcattcaacg agaaggagag tcatatgtct aaaacaattg caatcaatgc tgggagttca
    18781 agtttgaaat ggcaattata tcaaatgcca gaagaaaccg tagttgcgaa agggatcgtc
    18841 gaacgtatcg gtttacaaga ttctattttt accatcaagt acggcgataa tcaaaaattc
    18901 gaacaaatca tggatatcga aaaccacgaa gtagcagtaa aaatgctgtt agatcagttg
    18961 attgaactgc aaattttaag ctcttatgat gaaatcaccg gagtagggca ccgtgttgtt
    19021 cacggaggag aaacttatgg ggattcagta gtgatcgatg agaaagtaat ggaccgcatt
    19081 gctgaattag cagaatttgc tcctttgcat aatcctgcaa acttaatggg gatcaaagcg
    19141 tttaaaaaga ttttacctga cattttgagt gtggctgtat tcgatacatc tttccactca
    19201 acaatgccaa agcaaaatta tttatatagc ttaccggtgt cttattatga agatttcaaa
    19261 gtaagaaaat atggcttcca tggcacaagt catcgttatg tgtcggaacg tgcggcaaaa
    19321 ttattgaaca aaccgatcga agaactaaaa atcatcactt gccacttagg aaatggtgta
    19381 tcgatcacag cagtcgatgg aggtaaatcc atggatactt ctatgggatt cacaccatta
    19441 gctggggtga caatgggtac acgttcaggt gatatcgatc cagctgttct cccatattta
    19501 atggaaaaat tagagattga tattgatgaa atgatcaatg ttttgaacaa gaaatctggt
    19561 ctgctaggcc taacaggatt gtctagtgat atgcgtgatc ttgaaaagaa ctatgaaaaa
    19621 gaacatattc gtttagctta tgacattttc gtggaccgca tccgaaaata catcggcggt
    19681 tatgtgacaa cgatgaatgg tgttgatgca attgtcttta ctgctggtat cggcgagaat
    19741 gacggacatg tacgtagtga aatcatcaaa ggtatgtctt ggtttggttg tgaaatcgat
    19801 ccagcgttga atgaattgcg tggtgaagaa gtagatatct ctacaaaaga atcaaaagta
    19861 agagtattag tgatcccaac agatgaggaa ctaatgattg cccgtgatgt ggagcgtctg
    19921 cgcggctaag ccaaagcatt cgttaggata aaagatgaaa cgcatgcaag agggaattct
    19981 tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa tttaacttca atttcccttc
    20041 ttttttacag gatcttctta gggaattttg cttggaaaag tggtcaaaag ggaaaagact
    20101 ggcattttat ttattgattt tcaggaaaat cccaatattt tattttcatt catctttata
    20161 agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg ggctgtcgac
    20221 tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt gatcgaataa
    20281 taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc
    20341 aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc tggcggctga
    20401 tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat
    20461 ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt tcttctcgag
    20521 catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt ttttctaaaa
    20581 agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg atccgtttac
    20641 ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact tcttcgtttc
    20701 cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac tgcagctgct
    20761 gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg taaggctttt
    20821 cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg ccgatgatgg
    20881 ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact tgatcagcgt
    20941 actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca ccattagaca
    21001 agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc
    21061 ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca tgtgcccact
    21121 gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc agttcgtaat
    21181 tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg tagtttccgg
    21241 gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact tgatcgagaa
    21301 attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa tagttttcca
    21361 accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg
    21421 catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg ctaagttctg
    21481 ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg caagatcgct gcaatccggt
    21541 cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg taacgggcca
    21601 gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga ctcatttgct
    21661 gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg
    21721 aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga ctgataacac
    21781 cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt
    21841 tgataccgac taaggcaatt ttttcaccag cttcaatttg acaggataaa tcctcaaata
    21901 atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg atctctctcc
    21961 ttgagaccac tcaaaaaggc catggttatc catggcctgt gttatttaaa ctcagattat
    22021 catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg ttttggacaa
    22081 acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg
    22141 ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt ccctccttcg
    22201 tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc gctgacattt
    22261 acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga ctgagggtaa
    22321 tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg aaatcatatg
    22381 gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta ataccgttgc
    22441 attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg
    22501 ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt
    22561 gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa atcggtatcc
    22621 gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac attgacgaac
    22681 caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta atcgccctgg
    22741 tgttcacttt tttttatttt acttgaactc attgactgtt ttccctttat tttaaacatt
    22801 ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa
    22861 aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc tttagctggc
    22921 aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt tctaataaca
    22981 tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt ggctcctaac
    23041 ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg ttttggtgag
    23101 ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt gctttcataa
    23161 tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc atcaaaggat
    23221 gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc tccgttattt
    23281 cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga accatctacg
    23341 gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag tcaatcgtat
    23401 tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg ctttggttca
    23461 gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac gatcagtaaa
    23521 aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat tacgcctaaa
    23581 acagcatatg cagctccgta aaattgtagg aatccttgat ttttatcatt tttgttgatc
    23641 aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa taatactaaa
    23701 gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag caacaaatag
    23761 aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa tctccgttaa
    23821 tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt cccatggtcg
    23881 tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga cgggtcccaa
    23941 gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat cacagatttc
    24001 ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc agaaacagaa
    24061 ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac ggtaatactg
    24121 ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact gagcattctt
    24181 cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat gaggatactg
    24241 ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc tcaaatgatc
    24301 aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta atgccatgta
    24361 ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta gtgggatacg
    24421 tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac gagccgcagc
    24481 agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc ggcaaatact
    24541 tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat ccacatcact
    24601 tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc catttttgtc
    24661 atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa gatgaatcct
    24721 tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca atgcactccg
    24781 caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta actggattgc
    24841 ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca actggatgtc
    24901 ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat
    24961 ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac tttctaaatg
    25021 aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca ataagaggat
    25081 cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt ttgaatctgt
    25141 agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac ttgcgatact
    25201 cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc gagcatcgac
    25261 catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga cttcatcgat
    25321 cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct ccttcttgtt
    25381 tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag ccttctgttg
    25441 ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg agaaaaatga
    25501 cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa gtggcacggg
    25561 atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct ggatatattg
    25621 tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat ggtttaagaa
    25681 gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt tacacgatgc
    25741 atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa ggagtaatga
    25801 tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa
    25861 ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca attcctcaag
    25921 aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag aaaaaaaaga
    25981 caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt gtatggacag
    26041 aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa agaaaaaacc
    26101 aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct attaacatat
    26161 ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga ttgggataca
    26221 aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc acaatttgag
    26281 attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga agacggcgta
    26341 cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc agtaatccct
    26401 tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga atatctaaat
    26461 gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga cgatgacgaa
    26521 gctgtattcg agcttcactg ggataatcag caactagcag aattagtaga agtaaaacag
    26581 caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac agatatgaaa
    26641 tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc taatattttg
    26701 atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga acattttcag
    26761 tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa agaaggcgca
    26821 ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa
    26881 gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa agtaacagtt
    26941 agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca tccggtgcgt
    27001 attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga agatcctcta
    27061 gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca tcccacgact
    27121 tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg
    27181 ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa agatgtctat
    27241 gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa
    27301 atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga gattgaaagt
    27361 gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc agatgcttgg
    27421 cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa
    27481 atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa
    27541 gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg gtatttttta
    27601 aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta tcaccacatt
    27661 gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa tgatcaaata
    27721 gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa agaaattaaa
    27781 aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg ctatcctaaa
    27841 ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca agcttttctt
    27901 ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa
    27961 caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca gatcgtgcca
    28021 tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga ttatgacgtg
    28081 gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt
    28141 cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga aggtggatta
    28201 tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg attaggacca
    28261 agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag ctatcaatgg
    28321 gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta tcgacagaaa
    28381 gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac aatggatacg
    28441 aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc
    28501 acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt accgaaaaaa
    28561 gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg gtgtcataaa
    28621 gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag catgcgaata
    28681 cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca ttattcatcc
    28741 tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag tggctacagg
    28801 cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga gagaagaatt
    28861 tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa
    28921 atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga
    28981 tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta cgttgaagca
    29041 cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct atgcgcctct
    29101 tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag cacttcgtta
    29161 tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga gagaagaacg
    29221 tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag agctggggat
    29281 ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta aaatgaaaga
    29341 tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga tcgtagattc
    29401 aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac caatgcccgg
    29461 cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt tgcatactac
    29521 cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg aaatgcatga
    29581 aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt
    29641 agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg aacttcaaga
    29701 cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct ttagtgataa
    29761 agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat ccggaccatt
    29821 ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg caaaagtaaa
    29881 tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg agatcatgac
    29941 ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc
    30001 aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg
    30061 ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag atattttgac
    30121 gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag atgatctcta
    30181 tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc tgactgaaaa
    30241 agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca
    30301 gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg
    30361 ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat
    30421 cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt gtccgaatgt
    30481 tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg aagatacatc
    30541 gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc gttcaggctt
    30601 attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc
    30661 acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc agaatttatc
    30721 ccacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca gtgtacgccg
    30781 gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt aaagctgctg
    30841 tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt ttattaggca
    30901 ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc
    30961 gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg ggaggacaaa
    31021 ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat cgaccaagct
    31081 ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc aatgaaggaa
    31141 tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg tcggtttctc
    31201 tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa agtaatctgg
    31261 ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa ctctaactta
    31321 agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc
    31381 ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag taccagtagt
    31441 agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga
    31501 gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata aagaagctat
    31561 cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa
    31621 aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag tacagacttt
    31681 ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga caccggaaag
    31741 aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg aagcacagca
    31801 tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta tttgcgagtg
    31861 ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag cagaatatca
    31921 aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag gtgtcgaaaa
    31981 tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt
    32041 ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt tagatggtca
    32101 gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc caacggttgc
    32161 tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag atttgtatca
    32221 gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa tgagacgcta
    32281 ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag tagatggtta
    32341 tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc atgatgaacg
    32401 aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag caaaagatat
    32461 ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc gacaaatgca
    32521 tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg atcagctagt
    32581 aaataagttt gagttctctg tacctgaaca agattggacg attccattca atcagtttcc
    32641 gacttatccg aatagccgag cgctacttta caagaattag attagcataa acttatcgaa
    32701 aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa attgataagg
    32761 tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc attggataaa
    32821 tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc tccaggtata
    32881 actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc aggagtggct
    32941 tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt
    33001 tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg tttttcctca
    33061 ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg aacaaaactg
    33121 attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg gatcaaactg
    33181 tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa ttgttctaac
    33241 gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc gtgcaatgta
    33301 gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa aatgtcgatg
    33361 taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa
    33421 ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat gacgacacgt
    33481 gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga acggccgttt
    33541 ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt acgaactact
    33601 tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac ggcatttgct
    33661 cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc tgccatagct
    33721 gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta aaacgttttt
    33781 gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta ggttataagt
    33841 aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc
    33901 gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat ggacgagtga
    33961 aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt caaaattagg
    34021 tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg atttttttat
    34081 ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg
    34141 atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca cgtttattat
    34201 tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat gaagtgatat
    34261 cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat ttttatgaca
    34321 aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt cgttcttttg
    34381 ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt tatatgggac
    34441 caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat caaatcggag
    34501 tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg atggcacttg
    34561 atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca ttaggtgata
    34621 aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct tttgaatcag
    34681 agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta ttggacagta
    34741 aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat tatctaagcg
    34801 agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg gctgttcctt
    34861 atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg atttttgaaa
    34921 tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc ggacgttatg
    34981 acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt gcaatgggta
    35041 tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca tttaatgagt
    35101 tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg aaagtggtcc
    35161 aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt aaagcaaaag
    35221 cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc ggtgaagatg
    35281 agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc
    35341 cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact ttattcgagt
    35401 aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt ccgaacaaca
    35461 attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc ttgggggagt
    35521 aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat
    35581 caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac
    35641 tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt
    35701 cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc catttcaaat
    35761 tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg
    35821 tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag cgattcgtca
    35881 ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga aatctacacc
    35941 agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt tctatgcgtt
    36001 gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg accgttatta
    36061 tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac
    36121 gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt acacagaggg
    36181 attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc catttcctcg
    36241 tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata cacgttttga
    36301 tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag tcttccaaat
    36361 ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta
    36421 ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag ctaaggggct
    36481 tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat tcatgggaga
    36541 agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg
    36601 cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac gtttaggaaa
    36661 agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg attggccaca
    36721 atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat tcacgatgcc
    36781 aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg cagaagctta
    36841 tgatatcgtc ttgaacggtt atgaattagg cggaggttct atccgtattc atactcgtga
    36901 gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg aagaacaatt
    36961 tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta ttgcattagg
    37021 attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag tcattgcctt
    37081 tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg tttcgccttt
    37141 acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga taaaaaggta
    37201 aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa aacactttat
    37261 aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg aaaattgggc
    37321 ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga tcgcatcagt
    37381 cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta ctggtctagc
    37441 tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg tatcaacaac
    37501 tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt
    37561 tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata ttttaccgga
    37621 aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg tgatgggact
    37681 aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata tcgtgagtat
    37741 cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact tcaatgcatt
    37801 gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca gtacgttgtc
    37861 tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga agatgcaggt
    37921 aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag
    37981 agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc agacagtatt
    38041 gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga aagcttctga
    38101 tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt tctatgagga
    38161 tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca gacgttcgtt
    38221 tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt ttttatttga
    38281 aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg atgatagaaa
    38341 aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc cgtatattac
    38401 gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa aggttaaaaa
    38461 acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct
    38521 tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg
    38581 tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa
    38641 tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta
    38701 tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc
    38761 gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt
    38821 tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag
    38881 tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc
    38941 gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga
    39001 tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc
    39061 gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa
    39121 agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt
    39181 tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag
    39241 gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag
    39301 caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg
    39361 gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg
    39421 ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac
    39481 tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag
    39541 tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag
    39601 gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa
    39661 cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa
    39721 ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg
    39781 agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt
    39841 cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg
    39901 gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag
    39961 atttgttgaa agacactgtt ctgattggac taggaacgaa tggctcattc tcggaagctc
    40021 agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag
    40081 taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat
    40141 acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt
    40201 atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg
    40261 aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat
    40321 agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc
    40381 gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac
    40441 gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt gcttcgcttc
    40501 ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat
    40561 atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc
    40621 caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag
    40681 agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga
    40741 gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc
    40801 tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc
    40861 atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt
    40921 gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga
    40981 cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa
    41041 ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc
    41101 agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt
    41161 gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta
    41221 cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta
    41281 ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa
    41341 ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc
    41401 atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag
    41461 aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa
    41521 ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt
    41581 taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga attacatact
    41641 agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa
    41701 ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt
    41761 aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac
    41821 acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag
    41881 acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat
    41941 cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt
    42001 gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg
    42061 tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa
    42121 ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc
    42181 ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc
    42241 aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga
    42301 tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat
    42361 acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt
    42421 catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt
    42481 gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa
    42541 ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac
    42601 gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg
    42661 cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt
    42721 cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg
    42781 atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa
    42841 aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg
    42901 aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat
    42961 gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc
    43021 ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt
    43081 gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa
    43141 gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg
    43201 gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag
    43261 cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa
    43321 acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt
    43381 tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg
    43441 ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa
    43501 aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt
    43561 tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc
    43621 gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt
    43681 gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat
    43741 gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa
    43801 caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc
    43861 acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag
    43921 ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc
    43981 agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat
    44041 gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag
    44101 ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt
    44161 gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa
    44221 gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat
    44281 cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag
    44341 gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa
    44401 tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata
    44461 gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat
    44521 gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct
    44581 gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa
    44641 aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag
    44701 ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg
    44761 gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa
    44821 aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga
    44881 gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag
    44941 acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag
    45001 ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa
    45061 caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca
    45121 gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa
    45181 aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa
    45241 ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca
    45301 gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc
    45361 aagatggcca atgcaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa
    45421 caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg
    45481 caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa
    45541 gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag
    45601 cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt
    45661 caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct
    45721 aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa
    45781 cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta
    45841 gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac attcccagat
    45901 acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa
    45961 aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt
    46021 tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg
    46081 caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt
    46141 tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt
    46201 tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc
    46261 aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag
    46321 agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct
    46381 ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa
    46441 gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc
    46501 atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca
    46561 gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact
    46621 atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc
    46681 cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc
    46741 agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt
    46801 ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg
    46861 tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca
    46921 acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct
    46981 caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat
    47041 cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc atcaccgaaa
    47101 aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact
    47161 tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat
    47221 caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac
    47281 ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc
    47341 cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa
    47401 aacgatcaag gaatacaatt ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa
    47461 tttggagaag attatttgct gcaagcattg aatgattttg attttttctt tatgcattat
    47521 cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat
    47581 caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa
    47641 gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct
    47701 attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc
    47761 cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggtga agtcatctat
    47821 tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca
    47881 gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact
    47941 ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta
    48001 tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta
    48061 gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt
    48121 cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg
    48181 agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa
    48241 tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga
    48301 acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat
    48361 gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac
    48421 caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt
    48481 cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga
    48541 cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc
    48601 ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga
    48661 aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa
    48721 ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta
    48781 gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa
    48841 aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca
    48901 ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt
    48961 atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg
    49021 caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg
    49081 gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta
    49141 gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa
    49201 aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt
    49261 ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg
    49321 gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg
    49381 actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt
    49441 acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt
    49501 tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc
    49561 atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat
    49621 cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag
    49681 gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt
    49741 aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga
    49801 gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa
    49861 agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc
    49921 agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca
    49981 ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa
    50041 ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa
    50101 catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt
    50161 tctcagccag ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga
    50221 gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat
    50281 ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga
    50341 gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt
    50401 gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc
    50461 tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt
    50521 tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa
    50581 gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat
    50641 caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt
    50701 tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag
    50761 gtttttctta ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt
    50821 tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac aaattaatca ttatggtaga
    50881 agtcgattga tttattttaa tgcaatctct gtggaatttt cgaaagattt atttttttaa
    50941 tacagtctca atataactat tcaaacataa aataacgtta t
//
LOCUS       DAZKCE010000006        50107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000006 DAZKCE010000000
VERSION     DAZKCE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.006"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..301)
                     /locus_tag="ACG8KQ_000296"
     CDS             complement(<1..301)
                     /locus_tag="ACG8KQ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9103271.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDK"
     gene            complement(303..500)
                     /locus_tag="ACG8KQ_000297"
     CDS             complement(303..500)
                     /locus_tag="ACG8KQ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9103272.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(894..2621)
                     /locus_tag="ACG8KQ_000298"
     CDS             complement(894..2621)
                     /locus_tag="ACG8KQ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9103273.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2614..3807)
                     /locus_tag="ACG8KQ_000299"
     CDS             complement(2614..3807)
                     /locus_tag="ACG8KQ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9103274.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3994..4638)
                     /locus_tag="ACG8KQ_000300"
     CDS             complement(3994..4638)
                     /locus_tag="ACG8KQ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9103275.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4732..4866)
                     /locus_tag="ACG8KQ_000301"
     CDS             complement(4732..4866)
                     /locus_tag="ACG8KQ_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9103276.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4868..7000)
                     /gene="feoB"
                     /locus_tag="ACG8KQ_000302"
     CDS             complement(4868..7000)
                     /gene="feoB"
                     /locus_tag="ACG8KQ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9103277.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6997..7470)
                     /locus_tag="ACG8KQ_000303"
     CDS             complement(6997..7470)
                     /locus_tag="ACG8KQ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9103278.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7871..8095
                     /gene="nrdH"
                     /locus_tag="ACG8KQ_000304"
     CDS             7871..8095
                     /gene="nrdH"
                     /locus_tag="ACG8KQ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9103279.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8254..10413
                     /gene="nrdE"
                     /locus_tag="ACG8KQ_000305"
     CDS             8254..10413
                     /gene="nrdE"
                     /locus_tag="ACG8KQ_000305"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9103280.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10463..11428
                     /gene="nrdF"
                     /locus_tag="ACG8KQ_000306"
     CDS             10463..11428
                     /gene="nrdF"
                     /locus_tag="ACG8KQ_000306"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9103281.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11517..12656)
                     /locus_tag="ACG8KQ_000307"
     CDS             complement(11517..12656)
                     /locus_tag="ACG8KQ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9103282.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12965..13678)
                     /locus_tag="ACG8KQ_000308"
     CDS             complement(12965..13678)
                     /locus_tag="ACG8KQ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9103283.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13932..14633)
                     /gene="nagB"
                     /locus_tag="ACG8KQ_000309"
     CDS             complement(13932..14633)
                     /gene="nagB"
                     /locus_tag="ACG8KQ_000309"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9103284.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14867..16399
                     /locus_tag="ACG8KQ_000310"
     CDS             14867..16399
                     /locus_tag="ACG8KQ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9103285.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16590..17792)
                     /locus_tag="ACG8KQ_000311"
     CDS             complement(16590..17792)
                     /locus_tag="ACG8KQ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9103286.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17874..18563)
                     /locus_tag="ACG8KQ_000312"
     CDS             complement(17874..18563)
                     /locus_tag="ACG8KQ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9103287.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18669..19037)
                     /gene="rplL"
                     /locus_tag="ACG8KQ_000313"
     CDS             complement(18669..19037)
                     /gene="rplL"
                     /locus_tag="ACG8KQ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9103288.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19090..19593)
                     /gene="rplJ"
                     /locus_tag="ACG8KQ_000314"
     CDS             complement(19090..19593)
                     /gene="rplJ"
                     /locus_tag="ACG8KQ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9103289.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19797..19924)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20003..20692)
                     /gene="rplA"
                     /locus_tag="ACG8KQ_000315"
     CDS             complement(20003..20692)
                     /gene="rplA"
                     /locus_tag="ACG8KQ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9103290.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20796..21218)
                     /gene="rplK"
                     /locus_tag="ACG8KQ_000316"
     CDS             complement(20796..21218)
                     /gene="rplK"
                     /locus_tag="ACG8KQ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9103291.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21561..22229
                     /gene="sdaAB"
                     /locus_tag="ACG8KQ_000317"
     CDS             21561..22229
                     /gene="sdaAB"
                     /locus_tag="ACG8KQ_000317"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9103292.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22242..23114
                     /gene="sdaAA"
                     /locus_tag="ACG8KQ_000318"
     CDS             22242..23114
                     /gene="sdaAA"
                     /locus_tag="ACG8KQ_000318"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9103293.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23205..24305)
                     /locus_tag="ACG8KQ_000319"
                     /pseudo
     CDS             complement(23205..24305)
                     /locus_tag="ACG8KQ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24397..25284)
                     /locus_tag="ACG8KQ_000320"
     CDS             complement(24397..25284)
                     /locus_tag="ACG8KQ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9103294.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25361..25906)
                     /gene="nusG"
                     /locus_tag="ACG8KQ_000321"
     CDS             complement(25361..25906)
                     /gene="nusG"
                     /locus_tag="ACG8KQ_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9103295.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26007..26177)
                     /gene="secE"
                     /locus_tag="ACG8KQ_000322"
     CDS             complement(26007..26177)
                     /gene="secE"
                     /locus_tag="ACG8KQ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9103296.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26201..26353)
                     /gene="rpmG"
                     /locus_tag="ACG8KQ_000323"
     CDS             complement(26201..26353)
                     /gene="rpmG"
                     /locus_tag="ACG8KQ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9103297.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26515..27159
                     /gene="cbpA"
                     /locus_tag="ACG8KQ_000324"
     CDS             26515..27159
                     /gene="cbpA"
                     /locus_tag="ACG8KQ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9103298.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27215..27718)
                     /locus_tag="ACG8KQ_000325"
     CDS             complement(27215..27718)
                     /locus_tag="ACG8KQ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9103299.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27731..27777)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28315..29280
                     /locus_tag="ACG8KQ_000326"
     CDS             28315..29280
                     /locus_tag="ACG8KQ_000326"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9103300.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29379..30551)
                     /locus_tag="ACG8KQ_000327"
     CDS             complement(29379..30551)
                     /locus_tag="ACG8KQ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103301.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30538..31683)
                     /locus_tag="ACG8KQ_000328"
     CDS             complement(30538..31683)
                     /locus_tag="ACG8KQ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103302.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31697..32621)
                     /locus_tag="ACG8KQ_000329"
                     /pseudo
     CDS             complement(31697..32621)
                     /locus_tag="ACG8KQ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32738..33817
                     /locus_tag="ACG8KQ_000330"
     CDS             32738..33817
                     /locus_tag="ACG8KQ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9103303.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33814..34442
                     /locus_tag="ACG8KQ_000331"
                     /pseudo
     CDS             33814..34442
                     /locus_tag="ACG8KQ_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34552..34779)
                     /locus_tag="ACG8KQ_000332"
     CDS             complement(34552..34779)
                     /locus_tag="ACG8KQ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9103304.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34882..37470)
                     /locus_tag="ACG8KQ_000333"
     CDS             complement(34882..37470)
                     /locus_tag="ACG8KQ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9103305.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37490..38149)
                     /locus_tag="ACG8KQ_000334"
     CDS             complement(37490..38149)
                     /locus_tag="ACG8KQ_000334"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9103306.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38198..38701)
                     /gene="trmL"
                     /locus_tag="ACG8KQ_000335"
     CDS             complement(38198..38701)
                     /gene="trmL"
                     /locus_tag="ACG8KQ_000335"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9103307.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38922..39788)
                     /locus_tag="ACG8KQ_000336"
     CDS             complement(38922..39788)
                     /locus_tag="ACG8KQ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9103308.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39897..40538
                     /locus_tag="ACG8KQ_000337"
     CDS             39897..40538
                     /locus_tag="ACG8KQ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9103309.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40600..41961)
                     /gene="mgtE"
                     /locus_tag="ACG8KQ_000338"
     CDS             complement(40600..41961)
                     /gene="mgtE"
                     /locus_tag="ACG8KQ_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9103310.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41979..42878)
                     /locus_tag="ACG8KQ_000339"
     CDS             complement(41979..42878)
                     /locus_tag="ACG8KQ_000339"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9103311.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42880..43677)
                     /locus_tag="ACG8KQ_000340"
     CDS             complement(42880..43677)
                     /locus_tag="ACG8KQ_000340"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9103312.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43655..44359)
                     /locus_tag="ACG8KQ_000341"
     CDS             complement(43655..44359)
                     /locus_tag="ACG8KQ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9103313.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44490..45066
                     /locus_tag="ACG8KQ_000342"
                     /pseudo
     CDS             44490..45066
                     /locus_tag="ACG8KQ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45184..45861
                     /locus_tag="ACG8KQ_000343"
     CDS             45184..45861
                     /locus_tag="ACG8KQ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9103314.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45940..47748)
                     /gene="pepF"
                     /locus_tag="ACG8KQ_000344"
     CDS             complement(45940..47748)
                     /gene="pepF"
                     /locus_tag="ACG8KQ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9103315.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47924..48922)
                     /locus_tag="ACG8KQ_000345"
     CDS             complement(47924..48922)
                     /locus_tag="ACG8KQ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9103316.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49148..49801)
                     /locus_tag="ACG8KQ_000346"
     CDS             complement(49148..49801)
                     /locus_tag="ACG8KQ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9103317.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15004 a        10511 c         8781 g        15811 t
ORIGIN      
        1 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
       61 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      121 taaaatatag ataattcacc aatctattta ttaaaggacc gggatctaat tcatattttt
      181 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      241 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      301 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      361 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      421 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      481 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      541 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      601 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      661 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      721 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttggacg tttcgaagat
      781 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
      841 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
      901 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
      961 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1021 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1081 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1141 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1201 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1261 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1321 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1381 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1441 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1501 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1561 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1621 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1681 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1741 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     1801 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     1861 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     1921 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     1981 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2041 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2101 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2161 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2221 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2281 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2341 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2401 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2461 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2521 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2581 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2641 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2701 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     2761 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     2821 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     2881 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     2941 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3001 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3061 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3121 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3181 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3241 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3301 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3361 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3421 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3481 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3541 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3601 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3661 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3721 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     3781 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     3841 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     3901 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     3961 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4021 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4081 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4141 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4201 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4261 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4321 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4381 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4441 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4501 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4561 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4621 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4681 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4741 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     4801 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     4861 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     4921 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     4981 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5041 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5101 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5161 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5221 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5281 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5341 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5401 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5461 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5521 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5581 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5641 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5701 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     5761 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     5821 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     5881 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     5941 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6001 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6061 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6121 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6181 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6241 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6301 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6361 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6421 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6481 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6541 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6601 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6661 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6721 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     6781 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     6841 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     6901 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     6961 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7021 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7081 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7141 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7201 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7261 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7321 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7381 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7441 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7501 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7561 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7621 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7681 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7741 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     7801 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     7861 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     7921 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     7981 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8041 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8101 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8161 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8221 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8281 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8341 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8401 tttctatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8461 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8521 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8581 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8641 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8701 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     8761 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     8821 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     8881 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     8941 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9001 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9061 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9121 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9181 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9241 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9301 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9361 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9421 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt
     9481 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9541 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9601 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9661 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9721 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
     9781 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
     9841 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
     9901 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
     9961 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10021 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10081 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10141 gcttatgata tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc
    10201 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10261 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10321 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10381 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10441 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10501 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10561 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10621 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10681 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10741 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    10801 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    10861 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    10921 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    10981 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11041 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11101 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11161 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11221 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11281 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11341 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11401 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11461 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11521 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11581 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11641 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11701 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    11761 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    11821 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    11881 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    11941 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12001 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12061 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12121 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12181 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12241 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12301 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12361 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12421 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12481 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt
    12541 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12601 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12661 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12721 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    12781 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    12841 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    12901 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    12961 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13021 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13081 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13141 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13201 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13261 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13321 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13381 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13441 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13501 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13561 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13621 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13681 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13741 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    13801 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    13861 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    13921 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    13981 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14041 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14101 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14161 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14221 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14281 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14341 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14401 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14461 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14521 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14581 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14641 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14701 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    14761 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    14821 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    14881 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    14941 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15001 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15061 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15121 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15181 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15241 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15301 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15361 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15421 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15481 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15541 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15601 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15661 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15721 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    15781 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    15841 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    15901 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    15961 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16021 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16081 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16141 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16201 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16261 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16321 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16381 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16441 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16501 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16561 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16621 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16681 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16741 ccaataaagt acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc
    16801 ccctgtactc gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata
    16861 atagctaaaa gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt
    16921 gtttgcataa caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt
    16981 cccacgatag gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg
    17041 aatgttcccc aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc
    17101 gaaaatgttc caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa
    17161 gcaactaaat gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc
    17221 tggacagaac gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa
    17281 gcaaataaat agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga
    17341 gaaagcaaaa tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct
    17401 tttggtcccg ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat
    17461 cctattaaaa aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac
    17521 agagagacgg cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc
    17581 cctaaaggaa ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat
    17641 atcccgactg cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg
    17701 atgtaataca tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa
    17761 gttgttaaag gttttttttc attactcttc attgatttcc cccatttcct atcttagttt
    17821 acaacttatg gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg
    17881 tgcttttgtt ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact
    17941 agcacagtaa cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct
    18001 tctccaccac acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg
    18061 atttgctgta gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata
    18121 accggtatca tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct
    18181 tgcacccaat gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa
    18241 attgcagtga tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt
    18301 ccaaagctca aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac
    18361 atcaccgctg tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt
    18421 gcgagtagct ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat
    18481 tctaaataca accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc
    18541 aatcggatta ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat
    18601 agtttaacat aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat
    18661 agtaaaaatt atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct
    18721 tcagcttctt ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct
    18781 ttagcttctt tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt
    18841 tggtctccag ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg
    18901 ccaccagcag cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata
    18961 gctttaacta ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt
    19021 tcaatgttca atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt
    19081 atgcggcaat taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc
    19141 cacgttgcgg actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt
    19201 tggcaatttt gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc
    19261 gcctttgatt tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc
    19321 gactacatct tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc
    19381 ttcaaggcca gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc
    19441 tacgttagca tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg
    19501 gtagtcaacg actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat
    19561 tgctgctttt tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga
    19621 tggaagagtt ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt
    19681 attattcatc acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa
    19741 ctcctttcgc tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt
    19801 cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat attcaataaa
    19861 cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat
    19921 taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat
    19981 caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa
    20041 atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt
    20101 ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa
    20161 cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag
    20221 ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg
    20281 gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag
    20341 gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt
    20401 cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa
    20461 aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt
    20521 gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa
    20581 attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac
    20641 ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc
    20701 ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga
    20761 gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt
    20821 cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc
    20881 atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg
    20941 tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc
    21001 ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg
    21061 atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg
    21121 atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga
    21181 atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg
    21241 tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat
    21301 ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt
    21361 ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa
    21421 aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt
    21481 caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat
    21541 aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg
    21601 gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa
    21661 tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat
    21721 ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga
    21781 tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg
    21841 aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc
    21901 taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc
    21961 agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg
    22021 ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca
    22081 atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga
    22141 taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa
    22201 atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag
    22261 aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa
    22321 tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag
    22381 taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg
    22441 gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa
    22501 caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt
    22561 tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg
    22621 tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg
    22681 ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag
    22741 cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga
    22801 ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt
    22861 gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt
    22921 ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg
    22981 atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa
    23041 cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa
    23101 ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa
    23161 caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc
    23221 gtatatttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct
    23281 ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa
    23341 atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc
    23401 tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca
    23461 atttttggca atacttcaaa ggacgcagga cctccatcta gttgacgtcc accatgattg
    23521 gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc tggcgattgt
    23581 attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt
    23641 gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca
    23701 cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga
    23761 ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct
    23821 ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc
    23881 atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc
    23941 gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta
    24001 tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt
    24061 gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat
    24121 atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca
    24181 tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga
    24241 ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa
    24301 gtcatagtag atccttcttt catcttcttg attacatata aatcatacta aacttacaaa
    24361 aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact
    24421 tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt
    24481 cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc
    24541 actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa
    24601 gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt
    24661 agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac
    24721 aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc
    24781 ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat
    24841 gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc
    24901 tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc
    24961 tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac
    25021 caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt
    25081 ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt
    25141 actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg
    25201 gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca
    25261 taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact
    25321 tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt
    25381 gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt
    25441 tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga
    25501 tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga
    25561 tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc
    25621 cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt
    25681 ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt
    25741 ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt
    25801 cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc
    25861 ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat
    25921 ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc
    25981 actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg
    26041 gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac
    26101 gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg
    26161 ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta
    26221 atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc
    26281 cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt
    26341 ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt
    26401 atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat
    26461 tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta
    26521 ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta
    26581 gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct
    26641 tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat
    26701 ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac
    26761 ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc
    26821 cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc
    26881 tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag
    26941 caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt
    27001 attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct
    27061 aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa
    27121 gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa
    27181 cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg
    27241 cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg
    27301 caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa
    27361 tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt
    27421 tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa
    27481 aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa
    27541 ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa
    27601 actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca
    27661 tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt
    27721 tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg
    27781 aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa
    27841 aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt
    27901 aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag
    27961 aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta
    28021 gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc
    28081 cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac
    28141 ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt
    28201 tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa
    28261 ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa
    28321 ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca
    28381 gaaagaattt taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa
    28441 tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg
    28501 atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac
    28561 ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct
    28621 tatattccag caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc
    28681 aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct
    28741 tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta
    28801 gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac
    28861 aacttaggat cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga
    28921 tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt
    28981 acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg
    29041 tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg
    29101 ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca
    29161 acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa
    29221 atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa
    29281 taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt
    29341 gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt
    29401 tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca
    29461 aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg
    29521 agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct
    29581 attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa
    29641 ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa
    29701 gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga
    29761 taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag
    29821 ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt
    29881 cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga
    29941 ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag
    30001 gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct
    30061 ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct
    30121 tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta
    30181 gaaaaggcat ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt
    30241 tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca
    30301 tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat
    30361 ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct
    30421 tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg
    30481 ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta
    30541 taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat
    30601 aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag
    30661 tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa tcggaatatg
    30721 aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa agctcatcgc
    30781 aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt
    30841 accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa caatcatcaa
    30901 ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag ctaacataat
    30961 ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg aaactaacga
    31021 ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg cttgctgatc
    31081 ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa tcagagtgaa
    31141 gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa aagatacttc
    31201 ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg ccactttact
    31261 cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca acgtctgact
    31321 cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat aaaaaccagc
    31381 caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct gtacttcaaa
    31441 taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc
    31501 tgtttgatcc gctttgatta caattccagt attaatcgtt tcgagattgc caccttgatc
    31561 aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg ccaatgtcca
    31621 aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa aatgccaaaa
    31681 cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa aagacatcat
    31741 tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct tgtaagattg
    31801 taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc tgaccttttt
    31861 tataagcgat tccatcagaa gctattgttc gctgacaaca cgttttctgg gagtaatggg
    31921 atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct
    31981 ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct
    32041 tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt
    32101 tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt
    32161 aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta
    32221 agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca
    32281 atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt
    32341 tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc
    32401 ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca
    32461 taatttaaca aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggtcct
    32521 aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa
    32581 tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa
    32641 atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa
    32701 accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa
    32761 cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc
    32821 tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc
    32881 tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga
    32941 tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga
    33001 ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa
    33061 ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg
    33121 aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca
    33181 agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca
    33241 aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct
    33301 gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa
    33361 agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag
    33421 tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc
    33481 tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca
    33541 gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca
    33601 tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac
    33661 gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg
    33721 tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca
    33781 gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat tattgagatg
    33841 atccatttgt gacgaaatcg ttacaaacca ttttagaaag cacgaaagag atccgtgtgt
    33901 taggcattgg tcattgtgcc aaggatgcgc tagtcttata cgaaacccat cgtcccgatg
    33961 ttttgctgac ggacatccgc atgcctaaac aaacgggcat cgatgctgcc aaagaaattt
    34021 tggctaaatt tcccaaagcc atccttttgc ttttgacgac attcaaagat gaagaataca
    34081 tccgcgaagc attttccatc ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga
    34141 ttattccttc tgtcaaatcc gcttataatg gacaggccgt atttggtaat gaaatcatcg
    34201 agactttcac tcaaatgatg aagaataaac cgacgattga tagtcatgct tatgcttctt
    34261 ttagtgaacg tgagcttgca atcatcaaag aagtagctgc cgggaaaaac aataaagaaa
    34321 ttgcagatgc cttgtacttg agtgacggca ctgttcgcaa ttacatcagc caattactag
    34381 aaaagctaga tttacgggat cgcacgcaat tggctatcta tttttatcag catttgcaat
    34441 aagtcttatc taacacaaaa aaacctgaat gatgctcctt cgcatcattc aggttttttt
    34501 cacatcttct attcaattgt cccgcaaacc atttcctcac gattgaaaga atcaaggatt
    34561 cgcagggctg ccaggattcg ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt
    34621 gtctaacgtt ttttctagtt ttgattcttc acgatcgtct cgttctacat caggactttc
    34681 attcaatttg aattcttttt ccatctcatc taacttttta aagtcattgc cagattcttt
    34741 tgctaatttc gctaatttat cttcggtttc tttagccata aagtaacgcc ccccttttga
    34801 tttcagctta gcatatcaca ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt
    34861 agctatttct catctttaaa atcaagcatt ttttgccggt ataaaatcgt agggagaact
    34921 gttttccata ccaatcatcg gatctactaa ctggttatcg atcacttctg ctgtaagaac
    34981 gaaatcttta gaaacagcag gttctacctc ttctttcttt tcttctccaa caaatgaaga
    35041 aaacagatct ggttccactg tttccattac ttggtttttc gtttcattcg tcccactgtc
    35101 atcttctaca ggtagctttt cttttacaac ccgtggctct tttgtatagt tcccatcttc
    35161 aaatggatca tttccgacca aagaatcttc ataagctgct agtttggtcc ggagagtgat
    35221 attcatttta tcattatcca tgatccttga tttcaacgta gtcatccggc ttgctgattc
    35281 attcgctaca caagtgacat aggcttgctc ttcccctaac aaaatcaaca gttccttact
    35341 gcgagtcact gccgtgtaga gcaaattacg ctgaagcatc cgctgatatt gatggaccat
    35401 gggcaaaata accatcttga attcactgcc ttgtgattta tggattgagc aacaataaga
    35461 aagagtgatt ttattccatt cgtttctttt ataagtgacc tcatttgcat caaactggat
    35521 aactaattca tccactttat cttcagaatc ttttgccaag atgatacccg tgatttctcc
    35581 catatctcca ttgaatacat tcaattcggg agtattgact aattggagga ctttgtctcc
    35641 tatccgataa acggtgtcgt tccaatgtac ttcctttttt ccaggtttcc ctggattgaa
    35701 tatctcctgc atcattttat ttaacgcatc gatccctgca ggtccccgat acattggtgc
    35761 taatacttgg atatcttgtg cacggaatcc cttatttttc gctttttcca caattttaga
    35821 gatcaagggt tcaatttgat aagcatcact tcggaaatag gaacgatctt tttgattctt
    35881 tgtgaaatct tttggcagac gtccttcttt gatctcatgg gcaagaggaa taatactaga
    35941 accatcacct tgccgataaa tctgattgag ttccattttg ggaatttgtt cgatttccaa
    36001 aagatcatgg agtacttgac caggaccgac agagggaagc tgatctttgt ctcctactaa
    36061 gataacttgc atattcgtcg gaatcgcttt gaataatgtg ttagccagcc atgtatcgac
    36121 catagacatc tcatcaacaa tcaaaagtcc gccttctaat tctttcgtac tgactgaagg
    36181 tgttttttcc cgtccattca gccccagaag acgatggatc gtactggcag gcagacctgt
    36241 cgtttcgttc attcgtttag ctgctcggcc agttggtgct gcaagtagaa taggaaaaat
    36301 ttcttgggta tattttgaag gatccaattc cagtccgttc aactctgcaa aaagattgac
    36361 gattccgttg atcacagtag ttttccccgt acctggtcct cctgttaaaa taaatagagg
    36421 ggaacggatt gcttcagcga tagcttgttc ttgcgaaggt ccatactgta tgccgaatat
    36481 tttttcgata cgccgaatat tcttctgcac ttctttttca ggataatgga tttctttttt
    36541 acgactgaga aggcgctgga tcgaattacc gatcccccat tcagaaaagt acaaactgtt
    36601 ttcgtaaaga ttagtctctt cttgttgtat ctttccttct tcaacaagtt ggataattac
    36661 agtagcaacc tgatcaggct ggatttcaat tgggcgactt gtttctaaaa tacggatcgt
    36721 ttgctgaagc aatgtctccg ccggtacgta tgtatctcct gtttgaactg catgctgaaa
    36781 aatctgatgc aagatagctg ctcgaattct tctcggtgaa tctgctgcaa taccgatttg
    36841 ttcggctaga ttatctgcac gtttgaaccc gattccttct atgtcttcga ctagctggta
    36901 cggattttct tcgatgatat ccaaagactc gttcttatag gcctggtaga tactgaaagc
    36961 taactgacta ccgaacccgt aacgattcag tcccatgatc acttgatcca tcccgtggtt
    37021 tagccgaatc gtctcagcaa gcatttctct ttttggcttc gtcaaaccgg agatttgttt
    37081 taataccgaa ggatcagcaa tgattttttc gatagcatct tctcccagca attctacaat
    37141 tttttctgct gttttcttcc cgattcctgg gaatttttcg ctggaaagat acgctacgat
    37201 cccatttgca gttgttggct tttcttgctc atatctttcg agtttaagct gccttccgta
    37261 tttaggatga tccactaatt ctccataaaa acgataagtc tcattttctt gaatatcgcc
    37321 gaaacttccg gtgactacaa tctctttttc acggtaatca gctgtggttt cactgatgtg
    37381 taccaaaagc actttgtaaa aattactagg attttgaaaa aaaatcgcgg caactgtacc
    37441 aacgaaataa ggcatttctt ctggctgcat cggaagccgc ctccttcttc tatagtaaag
    37501 gttctggtgc tgtttcagtt cctaaaaaat ctgtttggtt ccatactttc agttcatatg
    37561 gcaataatct gcaaggctct tcagtctcta aaatcgtcaa actactattg aacaatccac
    37621 ccatttcacg caactcactt agctcttttc ctgtcatcca ttgaatggct gcagttaaag
    37681 aagctccatg tcctacgaat aaaagtgggc catctttatg ttgggcaacc gcatctgcta
    37741 caacagtttc gatacgtgtg attgccgatt caatcgtttc tccgtgaaaa ggagaaggat
    37801 catacagatc taaatgatta cgcaaattcg gcagattatc cggatatttc tggtacattt
    37861 catcgatcga ctgaccttct aaagtaccca gccctagttc tcttagctta tctgtataaa
    37921 aaatctctac cggacgagca agctctttgt tgataccttc tgctgtatca cgtgctcgct
    37981 ttgctgtact ggaataaatt gctttaaaag gaacatattt tacctttttc ccaaatgctt
    38041 tgatttcttc gtaactttgt ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc
    38101 tttcctgatt ccattgtgtc ttcccatgtc gggtaaaata tagttccata cgcagcaaac
    38161 tcctgtcttt cttttatttt actctataat aaactgtcta atccagcttg tcattttcat
    38221 agtgatgcgt caattctaaa ttagggaatc cttgctggcg aagtgcttca tacactatca
    38281 ttgcagctgt attcgataaa ttcaatgagc ggacatgctc atcattcata gggatgcgta
    38341 aacacttttc ttcgttttca cgcataaatg tttctggcaa accggttgtt tcttttccaa
    38401 ataaaaagaa gtgctcttct ccatcagcaa aattttcgtc actatatact ttatgggcaa
    38461 attttgagat caaatgtagt ttacgattac ctaaaaatgt taaaaaagct tctaaattac
    38521 tgtggtaagt aatatttaca tcattccaat aatccaaccc tgcccgtttt agatgtttgt
    38581 catcagttga aaaacctagt ggctcgatta aatgaagcgg agtattcgtg gctgcacacg
    38641 tacgggcaat atttccagtg tttgcaggga ttaatggttc aaataaaacg atatgattca
    38701 tcaggatcat tcctctcata tttatgttcg tttctatttt ctgacaattt gatttttgca
    38761 taaaaaaaaa cgtatcaact cggtgtcagt cactgcgagt cgttacgtta gtgaagctct
    38821 tctcactaac ttttatcaga acaggaacct gataaaaacc aatgaaaaaa caacttgttg
    38881 ctaatataac accctcaact tttttttgca acctctactc attaaaaact gctagaaaaa
    38941 cgttttctct tagttccttt taataattct atgtcaattg taatcttctt caaaggattc
    39001 ttatataagt ctgctttgat tcgctcttct acctgccatt ctaaaggtga catggccaaa
    39061 agatcttttt gattttcctc tggtatctca aattcatagg ttacacgctg acgttcagtt
    39121 aatttcattg cttcagcaaa cttggctact acacgttcat tggaataact ctgtttttca
    39181 ggttgattcg gataaaaagc ctgacgtagt tcctttaaat aattttcttg tggcaccacc
    39241 ttcaacactt ctccatcttc tgagagtaca cgttggaatt cttggtaatg agaaggcgaa
    39301 aagatattta aaaccgcgtt gaatgtttga tcagcaaacg gcaaattggt caaatcagct
    39361 acacaccaaa atgcttcggc agattgttct gtagctgcgt agactccttc ttttgaaata
    39421 tcaaatccaa cagcattttg aatctttttc aattttgaaa gttcattcaa gaagctacct
    39481 tctccgcagc caacatctaa tagcgagact tcttcaggta aatgctgcgc aatagtctca
    39541 ataacgggct gatacatgcc agattggatc attctacgcc ttggaacgaa cattccttga
    39601 tcgtattctg ttttaaccgc atgggataaa aaataaagtg ttcctttttt ggaaaggtca
    39661 aaacggtgat tatttttgca tatcaagcca ttatcagact tttttacttc ttcttggcaa
    39721 accggacagc ggaacagctg atgattttct gccaaaaact ggcgtcctcg gtcaattttt
    39781 tttaacatag ttccccctca tttctgtttt atcttaccgt tagatgctca ctttggcaac
    39841 tgctaaaaat atgatacaat gcctagaaga aagtgaaaga taaaggagaa gattagatga
    39901 tcaaagaatt ttgcgctgaa aactacacat ctattcctct agcaattgct agaggagccg
    39961 atcggatcga attgtgtgac aacttagcgg tcggtggcac aaccccgagt actggtgtga
    40021 tcgaagaaac gttaagttat gctggggaaa aagatatccc cgtaatgacg attattcgtc
    40081 ctagaggggg cgacttcgtc tataacgata tcgaattgaa aatcatgcat actgatttga
    40141 ttgaagcaaa gaaattaggg acggatggcg tagtatttgg ttgtcttact ccttcaggct
    40201 ggctagacga agaggcctta gaactattga tcgaaacagc agaagggttg cagatcacct
    40261 tccatatggc ttttgacagt attcctgaag atcggcaatt cgaagcaatc gattggctgg
    40321 tagaacatgg tgttgaccgt atcttgactc atggcggtcc tgctgacaaa actattgtag
    40381 aaaatttccc acggcttaaa gaattgattg cctatacaga tggccgctta atcattttgc
    40441 ctggtggtgg tgtcactgca gagaatgcag cacatgtagc taaagaattg aacgtaagcg
    40501 aagtacacgg aaccaaaatc gtggatcttc agttataatc ttataaacaa aaaaacattt
    40561 gcaacaggca taatgcttat tgcaaatgtt tttgtttttt tacataaaat agcccataaa
    40621 taaagaagca atattgaaat aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa
    40681 gggaccgctt gctactgctg gatcaaatcc tagacgatcc attagcatcg ggattaaact
    40741 acctgcaaga ttcgccactg tgattgcaaa tagcatagcc atccctatga caaaccccaa
    40801 gataaaatta tgtttccaaa aacctacgat tagaaaaatc gtgatccccg taacagctcc
    40861 tgtgacaacg ccggtcaaga cttcagccaa aacaagtcga acaaatccat gatctttctc
    40921 atcagaaatc gcaagtcgac gaacagccac agctagcgac tgggttccag cattaccggc
    40981 agttcctgta atcagcgaaa tgaagactgc taaaatactc gcttcactta ccaagtcttc
    41041 atagtgactg attaaagaag cggtcgacat acctaaaaac aacagagtga tcagccaagg
    41101 caaacgtcga gacgctgcct ttactggatt ttcattaatt tcttccacat tgaccccggc
    41161 aagtcctgaa taatcgctag cagcttcatc atcaataacg tcgatgatat catcgactgt
    41221 gacgattcct aacaaatgat catcataatc ggtaacagga agagctaaga agtcataatc
    41281 tctaaatgtt tgtgccacat cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc
    41341 gctcatcaga tcagaaatca ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat
    41401 aacccctact agctgacctt cttgattaac gacatagatg taatagattg tttcagctac
    41461 atctgcttcg ttcttcagca cgtacatcgc agagcgaaca gtttgattgg ctacgatcga
    41521 aacaaattca gttgtcatga tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt
    41581 gatttcactg gcatcgtctg ccgacatcaa acttaaatat ttagcaattt gctttttatc
    41641 cagcgtatta agcaagtcaa cagcattatc tgtatacatc tcagaaagca tatcagctgc
    41701 atagctagga cgcatttcat ctaggtattc tttcatatgt tcgttgtctt cttcaatcac
    41761 atcgaacata tccgctaatt ctttaggcga cagataggaa tagatcagct gcctgttctc
    41821 ttctgtaaga gattggtaga attgcccctg ctcatagata tgcatctcta aaaaactgtt
    41881 tctgaatgcc tgaatattat tttcccttaa atcttgagtc aactgggcaa atctttcctc
    41941 taattcttga ttttcatcca aaccaatctc acctcgcctt attcttcaaa ccgtctcaca
    42001 actgcctcca tgtctgcggc taaaggctgc ttgaactcca gcaactcttc tgtaaatgga
    42061 tggtaaaaac gcagatcata acaatgaagg gcttgtcttt ttattcccag atccattcgc
    42121 ccgccataca tatcatcacc gagcagggca cagccaattg cggaaaaatg aactcttatt
    42181 tgatgtgtcc ttcctgtatg cagctggata tcaactaacg cgatttctgt tagtcgcttc
    42241 tctatccagt actcagtctg tgccgaacgt ccttcatcag agaccattcg cttcaatagc
    42301 gaagaataat ctcgggcgat cggcaaatcg atatgtccat gtgccgataa atctgaaagc
    42361 cttccactga ctaaggcctg atattttttc acaaacttct tttcacgaag ttgcatatct
    42421 aattttgcgt gcgcaaatcc atgctttgca aaaagcatca aaccagatgt atcccgatct
    42481 agtcgcgtca ccacatggat tacttggttt tcgtaacctt gttttttata ataagccttt
    42541 acacgattag ccattgtccc atttggatgg tattgggcag gaatagacga aacgcctgct
    42601 ggtttattta ctaccagaac gtgttcatct tcatacacga tatcgatcgg tgtttcatcc
    42661 aaaagtacgg tttcatgttc tccttcagca ggaatcacaa tggttacctt atcatcctta
    42721 gctagagaaa acaagacatt ttctacttga tcattaactt tgatttgacc gccttgaaat
    42781 tttattttcg ctaacagacc ctttgagatc cctttttctt ttaaaaaata ttttacttgc
    42841 tgagaatgat ctttctcata atcccatgta aactgcatat caatcctcac taataaaggc
    42901 atctttcaca cgatgccaaa aatgcatatg tcgataagaa gcaaaatgga tccgttcttc
    42961 tgctattcga taataaattg agcggatatt tgcttttgcg acattcaatt gatcaaccgt
    43021 tacaagataa tcttctgtat tttccaattt tatttctagc cattcatcat gggcaatcac
    43081 gatcggagaa cccaaagtac gaaacactcg attattcagt gaagcaatct ctgccaactg
    43141 aaaggcattg atactaggat ggatcacagc accgccgaca cttttattat atgctgtcga
    43201 accagtaggc gtagaaatcg tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt
    43261 gatgtataca tcggctacca tcgtacgatt ggcccgttta atcgttgatt cattcagtgc
    43321 taaaaaatgc tggtccggtg tttcatccaa ataactgatc cgaacatcca gcaagggata
    43381 actaacactt tgttcccggt tcgttcgtaa gctgtctact agttcttcca gctcgtaatc
    43441 ccgccaatcc gtgtaaaacc ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt
    43501 cagacaatga ctgtagtgat ggaacgccga caataatgtg ccgtctccgc cgactgagat
    43561 gaccaattct ggctcatgat catctcgttg aatccctgct tgttctaaaa gtgcgttcaa
    43621 acgttctgtc acttctatag tctttgcttc tttattatga acgatcgcta ctttcatccc
    43681 ctgcctcctc tctttcgctt tctttcttca aaagcgttcg atagtagaca tcatctgatt
    43741 ttcctcttcc atgggagaat aaccgctgtg cttcttggat ttcttcacgg atactggaca
    43801 tctcttcatc caactgatag gcagcttctg ccgcacgctg aagtcgttca ctcatttctt
    43861 ctggaaaaac gccttgatat ttataattca gcgaatgctc aatcgttgcc cagaaattca
    43921 tcgcaagagt ccgaatctgg atttccgcca aaattttctt ctcaccgctg atcagctgta
    43981 caggatactc gatcaccaga tgataagaac ggtagccact tgctttttta tttgtgatgt
    44041 aatctcgttc ttggagaact ttcatgtctt ttcttttacg gatgatctca acaacttgat
    44101 ggatatcttc tacaaactga cacatgatcc ttaacccggc aatatcagac atttcttctt
    44161 ctaaacgtcc cattggaata tgccgtaatt gtgattttgt tacaatacta tcgactggct
    44221 tgactctccc agtgacaaat tcaatcggga catgtcgatt ttgttcacgg aactgtttgc
    44281 ggatcccccg cagtttcact ttcaattctg atacagtttg ttcatatggt gctaaaaatg
    44341 cttcccaatc tcgttccata gctgatacct ctacttcatt aacattcatt gtttattcta
    44401 ccatatatgc aaagtttttt cgtactcttg atttatgcat tgaccaaaaa aagaaaatta
    44461 ggcaaaatag acaaaaagga gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat
    44521 ctatgctgac aaagcaagag tacgagatat tcatcgctca ctatcaatta acagataaag
    44581 atttccgtgt acagactaat atttatttcg ataccgttga tgaacagctg aagaaacaaa
    44641 attgtggcct acggatccgt ttcgttggtc atcaagctga atatacatta aaaacacctg
    44701 ctgaaaaagg gcgtctcgaa acaacggata cctttataac agaagaagca gaagcattta
    44761 tcttcgaaaa acgtctgcct cgttcaggcg cagtttttca aaaactttct gatttgaata
    44821 tcgatcctgc ttcattaatc caaaccggaa agcttacaac aaaacgggct gaattcccta
    44881 ttgaagaagg acttttagct attgatgaaa gctggggaga caatctacac gattttgaac
    44941 tggaactaga ggtaagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa
    45001 cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa
    45061 aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta
    45121 agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt
    45181 catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa
    45241 caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa
    45301 tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga
    45361 atttctcaga acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca
    45421 ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa
    45481 ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata
    45541 tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt
    45601 aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct
    45661 tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa
    45721 ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa
    45781 gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat
    45841 ttacacttgc tttctaatta aaaaaatacg tcgactccct tacaagagag ccgacgtatt
    45901 tttttcgttt ctattcgaga aaatcaaaaa tagaaagcct tataaacgat cgatcaattc
    45961 ttctaattca tttagacgtt gttcaaacat cgacattgca tcttcaatat atgcagcctg
    46021 tgtcatatca actcctgctt ttttcattac ttccacagga taatcgctgt tacctgcttt
    46081 taggtaagcc aagtagttct ctagtgcctc tggttcctgg tttagtattt tcttcgccag
    46141 tgctgaagca gcagaaaagc cagtcgagta ttggaaaaca tagtaattat aataaaaatg
    46201 cggtatccgt gaccattcga atttaatttc tgggtcttct tcgactgctg gaccataata
    46261 ttttgcattc aatttaccat aactatcact taggtattca ctagtcaatg gcacaccttt
    46321 ttcatcttcc gtatgcatga aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt
    46381 tcctttaaac ccatccaagt aatggttgag tacataagcc cgtacacgag ggtctttttc
    46441 tgtttccaat aaatactccg ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa
    46501 aaagatggag tagtcgccgt acacataagg ctggttcgaa cgagtgaaat aactatgaac
    46561 actatgtccc atttcgtgga caagcgtaaa tagctgatcc agcgtatcat gccaattcaa
    46621 taaaatatat ggatttgtgt catagcttcc cgaagaataa gcaccgcttc gtttcccttt
    46681 attttcgaca acatcgatcc aacgttcaga gaatgctttt tctacgatgg ccatgtattc
    46741 ttcacccatt ggtttcagtg cttctaaagc tttttctttt gcttcttcgt acgtaaaggt
    46801 aattggagct tcccctaaga ccggtgtata aagatcatac atgtgcagtt tttccacttc
    46861 taataaacgt ttccgtaatt ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac
    46921 gtctaccaaa gtatcgtata cactttcagg aatatgatta ttgctcaaag acgcttctct
    46981 ggcagagctg taattacgga ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa
    47041 agtagaagca aatgtatttc taaattgttc gtaaacactg tacaatccct taaatgctgc
    47101 ttcgcgcacc cttcgatccg tgctttctag caactgacca tacacgccat gagataattg
    47161 gactatttca ccattttctc cttcaatcgt tggaaaaact agatctgcat tattcaaaac
    47221 agcaaatgta tcacttgaag catcaaagat ttcacctgct ccagcaagaa gagattcctg
    47281 ctcagcagat aagacatggg ctcgattatc tactatttgc tggatataat ggcgatagac
    47341 ttccaatttt ggttcttctt tgaaatattg ccaaatctgg tcatctgaca attgcaatat
    47401 ttctggttca aaccaggaaa cggcttcact aactttcgaa aacagactgc ttgctcttgc
    47461 ataaagcgct tggtaatctg tatttccagt gtcttgatcg tttttaaggt gcgcatatac
    47521 ataaatgact tcagtttggc gataaaccct caatacgaat tcaatcgcat ttaaaaattg
    47581 agaagcgcct tgatcaagtg ttcctttgtg tttttcagat tgttttaact cttctgataa
    47641 ttccaaatat ttctcatcaa actcttggtc gcttgaaaag atcttggtca agtcccaagt
    47701 taaattttca ggcaattctt ctcgttttgg caactgcttt acttccatga aatccactcc
    47761 ttttcttccc ttagctctat ctatcaaaat agatcatagt ctaacatcca catgtcacag
    47821 catctaactt ttctactcat tcatgtgtct gcttccatca attttagcat aactaaatct
    47881 tgttttacat agaaaaccga ttcctagcct atttttatac ttactatttt ttcaaagaag
    47941 acaagtgtat gcattcgtta aaaaactgct gatagaccat ctcttcatca atcataggaa
    48001 aaagccattc ccttttatgt tcttttattt ggcaaagcca tgagttgaaa tcaggaactt
    48061 tctggttttc caaggcttct tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat
    48121 aaaagaaaaa ccgacttttt tgatagatcc acgatgatag atgcaaaaga tgtttatgac
    48181 gcaagtagca ttgttcttgt atggatactg tattcttttg cttgtgaata agttggcgtg
    48241 tcagccattt cttcccatct tcactcactt tttctatttt tttcatttgg ttcattttaa
    48301 tttcgttact agtgaatatt tctttaaggt ccctactaga aaaagtccag cagctactgc
    48361 aaaaggaaat atgccctgat acagtctctg taatatgatg atacaaataa agcttttgtt
    48421 tcttgagatc agctttccag caatgcactc ctctttttct attatagtag cagcaacttt
    48481 tttctatcaa tgagaattgc gattgtccaa gaaaagaatt accaagtatc caccatactt
    48541 ggtaactgtt attcaaataa ttttgggttc tttcaataaa tctctggtga gacagtctag
    48601 aacattggat ttctattgct agattcgcca acaaaatatc cggcctttgc cgcaacctcg
    48661 gcaaataggc ttccaaaaat actggttcta ctgactgtcc cagccaatcc atgaagcatt
    48721 cctttaactg taagtgctct tttgattctc tttcactaaa gaccgaacag ttatctgatt
    48781 tttgatgggc aaaatgagca cattttttct ttcccttttt caaaatcact ctttttcgac
    48841 agccacgaca ataatatttc tttccttttt ctgcatgttt agcaaaacag cacttattat
    48901 attcattcat tgcaagcagc atttttctcc ctctgaactt atttacgtct aagaatttat
    48961 ttacacgaaa aaagagggcg tgaaaaagct attctgcatc aagtaataag aacagctaaa
    49021 caaaaatagt tcccaatatt tttcgtttgg ttgggcttgt taccgcagat gcaagctttt
    49081 gaacacattt atacgaaaaa aagactgcga cacaattttt gtcacagtct cttactataa
    49141 tgctgattta tacaaaataa aatttcgttc gctcgatagc attacgcgcc atcagacatt
    49201 gtccatattc attcaatgtt tctggtgtac gatcgctctt ttcagcaaac tccaaaattc
    49261 gagcaatctg attctctaca tccgcttggt tgagattttc catccagaag tgaacgacta
    49321 ggtaatagcg ctcttcgaat aaatacaaat cagtccaagc agactctagt tcgacatctg
    49381 ttgcaagttg gatcacttgt tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt
    49441 cttctagggc gcttccagat aattcttcct ccggatcgct gccaataatc tgctgacgaa
    49501 gaaagtcact cacatcttct gaatcaacag tagaatcttc tgattgagaa atttcttcat
    49561 ccggaagatt tttactgata aacagttcta atccgtctcc ttttggcaaa acttggaaag
    49621 tgacagcttc gcttcctcgg aactcctctt cgatatcaac ttcttccaaa atactataaa
    49681 agaaattttc gatttctttg tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct
    49741 ctaaatcttc atgaccaatt agtacacgga tcgtattttc attaatatgt tccatttcca
    49801 ttttgctaca cctcactttg ccacaatgtt taatatagat tcattgtagc gaaatcagct
    49861 taaatgtaaa gaaaaaaaat ctcgctgtta agcgaattta ataaaaccag cggatttttc
    49921 aacctttcaa gctatcttat ttaactataa catttttggc agacgaatac tatatagccg
    49981 acttttcagc taattttccc tctctatttt tcgtgcaatc atcatcttct actacctttt
    50041 tatacatcta atcaagaata ctttttgtaa aaacaaaaaa acttggaaaa actatctttg
    50101 taatagt
//
LOCUS       DAZKCE010000007        48105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000007 DAZKCE010000000
VERSION     DAZKCE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.007"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..704
                     /locus_tag="ACG8KQ_000347"
     CDS             333..704
                     /locus_tag="ACG8KQ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9103318.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(978..1670)
                     /locus_tag="ACG8KQ_000348"
     CDS             complement(978..1670)
                     /locus_tag="ACG8KQ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9103319.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1673..2458)
                     /locus_tag="ACG8KQ_000349"
     CDS             complement(1673..2458)
                     /locus_tag="ACG8KQ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9103320.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2695..4095
                     /gene="lacG"
                     /locus_tag="ACG8KQ_000350"
     CDS             2695..4095
                     /gene="lacG"
                     /locus_tag="ACG8KQ_000350"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9103321.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4120..4437
                     /locus_tag="ACG8KQ_000351"
     CDS             4120..4437
                     /locus_tag="ACG8KQ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9103322.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4450..6123
                     /locus_tag="ACG8KQ_000352"
     CDS             4450..6123
                     /locus_tag="ACG8KQ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9103323.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6137..7768
                     /locus_tag="ACG8KQ_000353"
     CDS             6137..7768
                     /locus_tag="ACG8KQ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9103324.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8259..9824)
                     /gene="guaA"
                     /locus_tag="ACG8KQ_000354"
     CDS             complement(8259..9824)
                     /gene="guaA"
                     /locus_tag="ACG8KQ_000354"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9103325.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9883..10806)
                     /gene="coaA"
                     /locus_tag="ACG8KQ_000355"
     CDS             complement(9883..10806)
                     /gene="coaA"
                     /locus_tag="ACG8KQ_000355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9103326.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11002..11607
                     /locus_tag="ACG8KQ_000356"
     CDS             11002..11607
                     /locus_tag="ACG8KQ_000356"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9103327.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11915..12931
                     /locus_tag="ACG8KQ_000357"
     CDS             11915..12931
                     /locus_tag="ACG8KQ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103328.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13072..14373
                     /locus_tag="ACG8KQ_000358"
     CDS             13072..14373
                     /locus_tag="ACG8KQ_000358"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9103329.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14419..15081
                     /gene="deoC"
                     /locus_tag="ACG8KQ_000359"
     CDS             14419..15081
                     /gene="deoC"
                     /locus_tag="ACG8KQ_000359"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9103330.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15102..15500
                     /locus_tag="ACG8KQ_000360"
     CDS             15102..15500
                     /locus_tag="ACG8KQ_000360"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9103331.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15566..16648
                     /locus_tag="ACG8KQ_000361"
     CDS             15566..16648
                     /locus_tag="ACG8KQ_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9103332.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16860..18422
                     /locus_tag="ACG8KQ_000362"
     CDS             16860..18422
                     /locus_tag="ACG8KQ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103333.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18415..19548
                     /locus_tag="ACG8KQ_000363"
     CDS             18415..19548
                     /locus_tag="ACG8KQ_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103334.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19548..20504
                     /locus_tag="ACG8KQ_000364"
     CDS             19548..20504
                     /locus_tag="ACG8KQ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103335.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20603..21769
                     /gene="deoB"
                     /locus_tag="ACG8KQ_000365"
     CDS             20603..21769
                     /gene="deoB"
                     /locus_tag="ACG8KQ_000365"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9103336.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21793..22611
                     /locus_tag="ACG8KQ_000366"
     CDS             21793..22611
                     /locus_tag="ACG8KQ_000366"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9103337.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22628..23335
                     /gene="deoD"
                     /locus_tag="ACG8KQ_000367"
     CDS             22628..23335
                     /gene="deoD"
                     /locus_tag="ACG8KQ_000367"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9103338.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23633..24319)
                     /gene="gpmA"
                     /locus_tag="ACG8KQ_000368"
     CDS             complement(23633..24319)
                     /gene="gpmA"
                     /locus_tag="ACG8KQ_000368"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9103339.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24491..25072)
                     /locus_tag="ACG8KQ_000369"
     CDS             complement(24491..25072)
                     /locus_tag="ACG8KQ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9103340.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25205..25888)
                     /gene="rpiA"
                     /locus_tag="ACG8KQ_000370"
     CDS             complement(25205..25888)
                     /gene="rpiA"
                     /locus_tag="ACG8KQ_000370"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9103341.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26207..26620
                     /gene="rpsL"
                     /locus_tag="ACG8KQ_000371"
     CDS             26207..26620
                     /gene="rpsL"
                     /locus_tag="ACG8KQ_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9103342.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26704..27174
                     /gene="rpsG"
                     /locus_tag="ACG8KQ_000372"
     CDS             26704..27174
                     /gene="rpsG"
                     /locus_tag="ACG8KQ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9103343.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27268..29355
                     /gene="fusA"
                     /locus_tag="ACG8KQ_000373"
     CDS             27268..29355
                     /gene="fusA"
                     /locus_tag="ACG8KQ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9103344.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29527..30714
                     /gene="tuf"
                     /locus_tag="ACG8KQ_000374"
     CDS             29527..30714
                     /gene="tuf"
                     /locus_tag="ACG8KQ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9103345.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31121..31429
                     /gene="rpsJ"
                     /locus_tag="ACG8KQ_000375"
     CDS             31121..31429
                     /gene="rpsJ"
                     /locus_tag="ACG8KQ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9103346.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31460..32089
                     /gene="rplC"
                     /locus_tag="ACG8KQ_000376"
     CDS             31460..32089
                     /gene="rplC"
                     /locus_tag="ACG8KQ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9103347.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32117..32740
                     /gene="rplD"
                     /locus_tag="ACG8KQ_000377"
     CDS             32117..32740
                     /gene="rplD"
                     /locus_tag="ACG8KQ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9103348.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32740..33030
                     /gene="rplW"
                     /locus_tag="ACG8KQ_000378"
     CDS             32740..33030
                     /gene="rplW"
                     /locus_tag="ACG8KQ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9103349.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33069..33902
                     /gene="rplB"
                     /locus_tag="ACG8KQ_000379"
     CDS             33069..33902
                     /gene="rplB"
                     /locus_tag="ACG8KQ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9103350.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33945..34223
                     /gene="rpsS"
                     /locus_tag="ACG8KQ_000380"
     CDS             33945..34223
                     /gene="rpsS"
                     /locus_tag="ACG8KQ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9103351.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34246..34593
                     /gene="rplV"
                     /locus_tag="ACG8KQ_000381"
     CDS             34246..34593
                     /gene="rplV"
                     /locus_tag="ACG8KQ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9103352.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34607..35263
                     /gene="rpsC"
                     /locus_tag="ACG8KQ_000382"
     CDS             34607..35263
                     /gene="rpsC"
                     /locus_tag="ACG8KQ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9103353.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35266..35700
                     /gene="rplP"
                     /locus_tag="ACG8KQ_000383"
     CDS             35266..35700
                     /gene="rplP"
                     /locus_tag="ACG8KQ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9103354.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35690..35878
                     /gene="rpmC"
                     /locus_tag="ACG8KQ_000384"
     CDS             35690..35878
                     /gene="rpmC"
                     /locus_tag="ACG8KQ_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9103355.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35903..36169
                     /gene="rpsQ"
                     /locus_tag="ACG8KQ_000385"
     CDS             35903..36169
                     /gene="rpsQ"
                     /locus_tag="ACG8KQ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9103356.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36227..36595
                     /gene="rplN"
                     /locus_tag="ACG8KQ_000386"
     CDS             36227..36595
                     /gene="rplN"
                     /locus_tag="ACG8KQ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9103357.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36632..36940
                     /gene="rplX"
                     /locus_tag="ACG8KQ_000387"
     CDS             36632..36940
                     /gene="rplX"
                     /locus_tag="ACG8KQ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9103358.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36968..37507
                     /gene="rplE"
                     /locus_tag="ACG8KQ_000388"
     CDS             36968..37507
                     /gene="rplE"
                     /locus_tag="ACG8KQ_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9103359.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37526..37711
                     /locus_tag="ACG8KQ_000389"
     CDS             37526..37711
                     /locus_tag="ACG8KQ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9103360.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37747..38145
                     /gene="rpsH"
                     /locus_tag="ACG8KQ_000390"
     CDS             37747..38145
                     /gene="rpsH"
                     /locus_tag="ACG8KQ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9103361.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38177..38713
                     /gene="rplF"
                     /locus_tag="ACG8KQ_000391"
     CDS             38177..38713
                     /gene="rplF"
                     /locus_tag="ACG8KQ_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9103362.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38874..39230
                     /gene="rplR"
                     /locus_tag="ACG8KQ_000392"
     CDS             38874..39230
                     /gene="rplR"
                     /locus_tag="ACG8KQ_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9103363.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39251..39751
                     /gene="rpsE"
                     /locus_tag="ACG8KQ_000393"
     CDS             39251..39751
                     /gene="rpsE"
                     /locus_tag="ACG8KQ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9103364.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39766..39945
                     /gene="rpmD"
                     /locus_tag="ACG8KQ_000394"
     CDS             39766..39945
                     /gene="rpmD"
                     /locus_tag="ACG8KQ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9103365.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39989..40429
                     /gene="rplO"
                     /locus_tag="ACG8KQ_000395"
     CDS             39989..40429
                     /gene="rplO"
                     /locus_tag="ACG8KQ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9103366.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40429..41724
                     /gene="secY"
                     /locus_tag="ACG8KQ_000396"
     CDS             40429..41724
                     /gene="secY"
                     /locus_tag="ACG8KQ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9103367.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41786..42433
                     /locus_tag="ACG8KQ_000397"
     CDS             41786..42433
                     /locus_tag="ACG8KQ_000397"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9103368.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42624..42842
                     /gene="infA"
                     /locus_tag="ACG8KQ_000398"
     CDS             42624..42842
                     /gene="infA"
                     /locus_tag="ACG8KQ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9103369.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42875..42991
                     /gene="rpmJ"
                     /locus_tag="ACG8KQ_000399"
     CDS             42875..42991
                     /gene="rpmJ"
                     /locus_tag="ACG8KQ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9103370.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43009..43374
                     /gene="rpsM"
                     /locus_tag="ACG8KQ_000400"
     CDS             43009..43374
                     /gene="rpsM"
                     /locus_tag="ACG8KQ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9103371.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43402..43791
                     /gene="rpsK"
                     /locus_tag="ACG8KQ_000401"
     CDS             43402..43791
                     /gene="rpsK"
                     /locus_tag="ACG8KQ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9103372.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43871..44809
                     /locus_tag="ACG8KQ_000402"
     CDS             43871..44809
                     /locus_tag="ACG8KQ_000402"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9103373.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44837..45217
                     /gene="rplQ"
                     /locus_tag="ACG8KQ_000403"
     CDS             44837..45217
                     /gene="rplQ"
                     /locus_tag="ACG8KQ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9103374.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45489..46328
                     /locus_tag="ACG8KQ_000404"
     CDS             45489..46328
                     /locus_tag="ACG8KQ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9103375.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46304..47173
                     /locus_tag="ACG8KQ_000405"
     CDS             46304..47173
                     /locus_tag="ACG8KQ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9103376.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47163..47960
                     /locus_tag="ACG8KQ_000406"
     CDS             47163..47960
                     /locus_tag="ACG8KQ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9103377.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15215 a         8786 c        10531 g        13573 t
ORIGIN      
        1 atattactaa ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta
       61 tatttagtaa gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa
      121 gtttgtttct taattagctt attgattcta taatattttt gatttatata ggaaattaaa
      181 aatattatag aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg
      241 ataatgaaat ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc
      301 aagattactt tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc
      361 aagaagtcat caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag
      421 cagttgccat tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt
      481 atggcgataa agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga
      541 aggcagatct taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga
      601 cagagaaaga tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt
      661 tttcaactca gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat
      721 taataaaagt atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat
      781 gtgaaaacac tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa
      841 gaatgaaaaa gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc
      901 atgtcgtttc actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg
      961 gtagttagtt gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa
     1021 ccagtaaaat ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg
     1081 tttttaccga atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt
     1141 aatcctgagt aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct
     1201 gccgctttaa gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca
     1261 atcttgtccc agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc
     1321 tctttgacag tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga
     1381 gtcgcttcaa gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga
     1441 taatagcttt ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa
     1501 atgaaatgtt tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg
     1561 tctgataaac catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga
     1621 tgagagtcca aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc
     1681 ctatagtggt attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct
     1741 cttgaacctc ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat
     1801 aaaacacata aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac
     1861 gggaattatt tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg
     1921 cttcgttgtg aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat
     1981 tgtcgttggt gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca
     2041 gtagaatttc tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa
     2101 tgatccgaat tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc
     2161 ctaagaaaat cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt
     2221 tttcttgaat ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg
     2281 aaagactttg agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta
     2341 aatctctgcg aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa
     2401 ggattccttt tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg
     2461 tcttcacctc taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg
     2521 aacaaaagta aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt
     2581 tctggttttt atgattattt gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca
     2641 atgcaatcag ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt
     2701 aaattaccag aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc
     2761 actaaggaag gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga
     2821 ttcagtcccg atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt
     2881 gaacgttttg gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat
     2941 ggagctggga aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt
     3001 aagaaaagaa acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg
     3061 tttgaccatg gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt
     3121 ttttgttttc atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca
     3181 gttgcaacca atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca
     3241 aagatcgtcg cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc
     3301 aaagaaaacg aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg
     3361 gcaacagacg cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt
     3421 ttcttattag atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat
     3481 gagatatgtg aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg
     3541 aaaaaagcat ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc
     3601 aaagcttacg atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct
     3661 gtttataaag tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat
     3721 tgggactggc tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat
     3781 tacccacatt acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt
     3841 gaagatggca ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc
     3901 ttaagcaagg cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg
     3961 gatcttttct catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt
     4021 gaaacccaga agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc
     4081 aaaacgatta tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa
     4141 atgcttggtt ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta
     4201 ttgaaagaag taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac
     4261 gaaaatttaa cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg
     4321 aaagagatgg agatgggatt catttttatc catgggcagg atcatttaat gaccacttta
     4381 ttgctgagag atctgattca agactttatt gtactgtaca gacagaatca aggataggag
     4441 aagaacgcta tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa
     4501 atctcacgta atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct
     4561 attttatttt caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa
     4621 tggcctgaat ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta
     4681 gggttactcg tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta
     4741 ccgaaaaaca aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg
     4801 ttgttaagcg tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga
     4861 ctactggctt cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg
     4921 aaagatatta ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga
     4981 gatatttttc ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga
     5041 caaatcttcc aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca
     5101 gcagctgacg ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt
     5161 gtcggtgttc atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat
     5221 gtggaaacca atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg
     5281 ggtttaggga attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt
     5341 atcttcctac tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt
     5401 ccagtaagtt tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct
     5461 tattttttta ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt
     5521 gtcgatgtat tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg
     5581 atcggtttgg ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta
     5641 atcgtcgttg acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta
     5701 gagaaagaag cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta
     5761 cgtgaggaag tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat
     5821 gtgttggtac tttgtgctgg cgcagggaca agtgggatgt tagctaatgc tttaaaaaaa
     5881 ggtgcatctg aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac
     5941 gaaatgctgc aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat
     6001 gatatcaaag aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa
     6061 tatatcggat taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa
     6121 taaaggagga gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc
     6181 tgtgttaggg gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat
     6241 tcgcggagca gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga
     6301 agacgatatt tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg
     6361 actgcgtttg tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa
     6421 tgatctggaa acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct
     6481 gctggatttt cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc
     6541 atggcaaggt ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt
     6601 tctgtcggaa atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact
     6661 gaatagcgga atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga
     6721 tcgattggct gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac
     6781 aactccagta atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt
     6841 tgatgaaatc acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta
     6901 ttggcacggt tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg
     6961 taaagaagtg atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat
     7021 gccaaatgct tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg
     7081 acaagcagag tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg
     7141 ggcgggcttc ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca
     7201 agcaggtatg aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc
     7261 gatgtttgat tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc
     7321 gcaaataaat ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc
     7381 caattggcaa acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg
     7441 cacttttttt ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc
     7501 ttatcaaaat gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc
     7561 tgggggagag tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa
     7621 tactgcggtt gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac
     7681 aacaggtgtt tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt
     7741 tgattttgtt tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt
     7801 tcccttcagc cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc
     7861 aaaaataacc agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc
     7921 atctacactt tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa
     7981 ttgcaaaaag ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc
     8041 gtcgtgaata tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt
     8101 ttttcgagaa cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt
     8161 ttggtaggga accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt
     8221 gatttatagg gattccccct ccatattatg ttggctcttt attcccactc cactgttgct
     8281 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
     8341 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
     8401 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
     8461 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
     8521 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
     8581 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
     8641 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
     8701 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
     8761 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
     8821 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
     8881 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
     8941 ttcaacttgt ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt
     9001 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
     9061 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
     9121 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
     9181 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
     9241 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
     9301 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
     9361 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
     9421 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
     9481 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
     9541 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
     9601 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
     9661 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
     9721 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
     9781 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
     9841 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
     9901 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
     9961 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    10021 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    10081 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    10141 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    10201 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    10261 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    10321 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    10381 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    10441 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    10501 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    10561 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    10621 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    10681 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    10741 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    10801 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    10861 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    10921 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    10981 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    11041 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    11101 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    11161 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    11221 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    11281 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    11341 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    11401 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    11461 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    11521 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    11581 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    11641 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    11701 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    11761 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    11821 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    11881 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    11941 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    12001 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    12061 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    12121 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    12181 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    12241 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    12301 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    12361 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    12421 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    12481 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    12541 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    12601 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    12661 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    12721 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    12781 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    12841 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    12901 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    12961 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    13021 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    13081 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    13141 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    13201 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    13261 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    13321 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    13381 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    13441 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    13501 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    13561 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    13621 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    13681 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    13741 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    13801 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    13861 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    13921 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    13981 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    14041 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    14101 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    14161 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    14221 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    14281 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    14341 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    14401 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    14461 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    14521 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    14581 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    14641 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    14701 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    14761 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    14821 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    14881 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    14941 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    15001 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    15061 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    15121 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    15181 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    15241 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    15301 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    15361 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    15421 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    15481 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    15541 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    15601 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    15661 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    15721 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    15781 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    15841 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    15901 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    15961 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    16021 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    16081 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    16141 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    16201 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    16261 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    16321 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    16381 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    16441 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    16501 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    16561 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    16621 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    16681 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    16741 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    16801 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    16861 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    16921 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    16981 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    17041 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    17101 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    17161 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    17221 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    17281 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    17341 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    17401 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    17461 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    17521 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    17581 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    17641 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    17701 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    17761 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    17821 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    17881 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    17941 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    18001 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    18061 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    18121 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    18181 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    18241 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    18301 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    18361 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    18421 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    18481 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    18541 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    18601 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    18661 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    18721 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    18781 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    18841 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    18901 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    18961 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    19021 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    19081 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    19141 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    19201 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    19261 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    19321 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    19381 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    19441 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    19501 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    19561 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    19621 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    19681 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    19741 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    19801 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    19861 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    19921 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    19981 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    20041 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    20101 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    20161 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    20221 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    20281 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    20341 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    20401 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    20461 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    20521 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    20581 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    20641 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    20701 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    20761 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    20821 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    20881 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    20941 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    21001 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    21061 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    21121 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    21181 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    21241 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    21301 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    21361 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    21421 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    21481 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    21541 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    21601 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    21661 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    21721 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    21781 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    21841 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    21901 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    21961 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    22021 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    22081 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    22141 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    22201 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    22261 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    22321 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    22381 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    22441 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    22501 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    22561 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    22621 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    22681 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    22741 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    22801 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    22861 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    22921 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    22981 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    23041 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    23101 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    23161 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    23221 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    23281 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    23341 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    23401 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    23461 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    23521 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac
    23581 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    23641 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    23701 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    23761 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    23821 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    23881 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    23941 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    24001 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    24061 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    24121 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    24181 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    24241 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    24301 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    24361 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    24421 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    24481 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    24541 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    24601 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    24661 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    24721 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    24781 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    24841 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    24901 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    24961 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    25021 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    25081 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    25141 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    25201 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    25261 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    25321 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    25381 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    25441 tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg
    25501 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    25561 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    25621 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    25681 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    25741 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    25801 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    25861 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    25921 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    25981 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    26041 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    26101 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    26161 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    26221 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    26281 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    26341 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    26401 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    26461 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    26521 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    26581 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    26641 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    26701 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    26761 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    26821 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    26881 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    26941 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    27001 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    27061 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    27121 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    27181 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    27241 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    27301 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    27361 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    27421 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    27481 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    27541 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    27601 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    27661 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    27721 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    27781 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    27841 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    27901 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    27961 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    28021 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    28081 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    28141 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    28201 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    28261 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    28321 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    28381 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    28441 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    28501 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    28561 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    28621 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    28681 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    28741 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    28801 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    28861 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    28921 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    28981 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    29041 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    29101 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    29161 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    29221 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    29281 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    29341 ggaaaagctg aataatttct tgaaaccgga tagtcctgca aagagattat ccaaagagac
    29401 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    29461 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    29521 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    29581 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    29641 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    29701 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    29761 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    29821 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    29881 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    29941 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    30001 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    30061 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    30121 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    30181 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    30241 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    30301 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    30361 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    30421 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    30481 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    30541 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    30601 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    30661 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    30721 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg
    30781 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    30841 aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa
    30901 aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg
    30961 tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg
    31021 agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga
    31081 gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg
    31141 tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga
    31201 aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag
    31261 tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat
    31321 gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt
    31381 gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa
    31441 aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca
    31501 aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt
    31561 agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca
    31621 agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac
    31681 ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg
    31741 aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac
    31801 gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc
    31861 tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt
    31921 atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt
    31981 ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg
    32041 agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga
    32101 aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg
    32161 tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga
    32221 tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag
    32281 cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca
    32341 aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg
    32401 ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc
    32461 agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa
    32521 aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt
    32581 tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt
    32641 agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac
    32701 acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc
    32761 aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt
    32821 gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac
    32881 gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa
    32941 tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa
    33001 gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag
    33061 ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag
    33121 gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga
    33181 aaaaccatgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac
    33241 acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg
    33301 ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag
    33361 atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt
    33421 caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg
    33481 gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg
    33541 ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact
    33601 ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg
    33661 aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc
    33721 caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta
    33781 aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca
    33841 aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat
    33901 aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa
    33961 agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg
    34021 aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg
    34081 gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca
    34141 tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg
    34201 acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc
    34261 acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta
    34321 attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca
    34381 aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat
    34441 aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct
    34501 acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca
    34561 agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt
    34621 acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga
    34681 aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa
    34741 acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat
    34801 ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag
    34861 cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat
    34921 caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa
    34981 ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc
    35041 tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga
    35101 aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg
    35161 ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga
    35221 aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa
    35281 cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa
    35341 gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc
    35401 caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg
    35461 attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct
    35521 ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa
    35581 atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta
    35641 ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa
    35701 agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga
    35761 attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa
    35821 agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt
    35881 atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg
    35941 tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa
    36001 ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa
    36061 caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac
    36121 aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat
    36181 tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga
    36241 aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct
    36301 tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca
    36361 agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac
    36421 aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt
    36481 gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga
    36541 actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg
    36601 agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa
    36661 agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa
    36721 agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc
    36781 tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt
    36841 ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt
    36901 ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc
    36961 ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga
    37021 aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat
    37081 gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac
    37141 tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg
    37201 tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga
    37261 attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag
    37321 taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt
    37381 cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac
    37441 aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca
    37501 aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg
    37561 tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc
    37621 agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca
    37681 aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa
    37741 agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac
    37801 atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa
    37861 atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc
    37921 gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa
    37981 cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg
    38041 aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct
    38101 cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac
    38161 aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt
    38221 aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg
    38281 tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc
    38341 aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat
    38401 ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg
    38461 tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat
    38521 cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg
    38581 tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga
    38641 accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa
    38701 aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt
    38761 ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga
    38821 aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta
    38881 caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct
    38941 ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc
    39001 aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa
    39061 tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg
    39121 cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc
    39181 gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg
    39241 aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc
    39301 aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt
    39361 tgttgtcggt gacaaaaacg gacacgtagg atttggtaca ggtaaagctc aagaagttcc
    39421 tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt
    39481 tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa
    39541 acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt
    39601 agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt
    39661 tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg
    39721 cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa
    39781 gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg
    39841 ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc
    39901 atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata
    39961 actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg
    40021 ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc
    40081 cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga
    40141 agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg
    40201 taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt
    40261 aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt
    40321 attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc
    40381 agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta
    40441 ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg
    40501 ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg
    40561 caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg
    40621 caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt
    40681 cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt
    40741 ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa
    40801 tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct
    40861 aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact
    40921 tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt
    40981 gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata
    41041 aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg
    41101 gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc
    41161 caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat
    41221 gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt
    41281 ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc
    41341 tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact
    41401 ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa
    41461 ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta
    41521 ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc
    41581 attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg
    41641 atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt
    41701 aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct
    41761 cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg
    41821 taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg
    41881 agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta
    41941 tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact
    42001 agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca
    42061 agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga
    42121 tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa
    42181 ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg
    42241 ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg
    42301 tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt
    42361 gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat
    42421 cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag
    42481 ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca
    42541 ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa
    42601 acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag
    42661 tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc
    42721 ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag
    42781 taacggttga attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat
    42841 aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc
    42901 aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc
    42961 aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt
    43021 gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt
    43081 attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc
    43141 gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa
    43201 gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt
    43261 tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac
    43321 aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta
    43381 agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag
    43441 aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg
    43501 atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa
    43561 ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca
    43621 gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt
    43681 gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg
    43741 actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg
    43801 ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt
    43861 aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga
    43921 ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc
    43981 cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga
    44041 tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat
    44101 cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat
    44161 cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga
    44221 aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg
    44281 cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat
    44341 gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca
    44401 agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg
    44461 gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga
    44521 acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga
    44581 aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc
    44641 tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa
    44701 taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt
    44761 taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg
    44821 aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc
    44881 gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc
    44941 tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga
    45001 tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga
    45061 agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc
    45121 tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg
    45181 tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt
    45241 atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc
    45301 gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat
    45361 accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag
    45421 aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag
    45481 gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg
    45541 atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta
    45601 ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc
    45661 tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga
    45721 cgatccgaca gatggttgga atggtttttc aaaatccaga caatcagttt gttggttcga
    45781 cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc
    45841 tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc
    45901 ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac
    45961 gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag
    46021 aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca
    46081 ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac
    46141 ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag
    46201 gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg
    46261 tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga
    46321 acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat
    46381 caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa
    46441 atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat
    46501 tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa
    46561 agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga
    46621 cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa
    46681 agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact
    46741 ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt
    46801 acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga
    46861 gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat
    46921 ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa
    46981 tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg
    47041 cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac
    47101 cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc
    47161 ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat
    47221 tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa
    47281 ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa
    47341 aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca
    47401 ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt
    47461 tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga
    47521 tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga
    47581 ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga
    47641 tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga
    47701 acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag
    47761 cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg
    47821 cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat
    47881 tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac
    47941 tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct
    48001 gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta
    48061 aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaa
//
LOCUS       DAZKCE010000008        46114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000008 DAZKCE010000000
VERSION     DAZKCE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.008"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..964
                     /locus_tag="ACG8KQ_000407"
     CDS             83..964
                     /locus_tag="ACG8KQ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9103378.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            977..2587
                     /locus_tag="ACG8KQ_000408"
     CDS             977..2587
                     /locus_tag="ACG8KQ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103379.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2664..3323)
                     /locus_tag="ACG8KQ_000409"
     CDS             complement(2664..3323)
                     /locus_tag="ACG8KQ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9103380.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3606..4271)
                     /locus_tag="ACG8KQ_000410"
     CDS             complement(3606..4271)
                     /locus_tag="ACG8KQ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9103381.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4418..5023)
                     /gene="dhaL"
                     /locus_tag="ACG8KQ_000411"
     CDS             complement(4418..5023)
                     /gene="dhaL"
                     /locus_tag="ACG8KQ_000411"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9103382.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5051..6061)
                     /gene="dhaK"
                     /locus_tag="ACG8KQ_000412"
     CDS             complement(5051..6061)
                     /gene="dhaK"
                     /locus_tag="ACG8KQ_000412"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9103383.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6036..6431)
                     /gene="dhaM"
                     /locus_tag="ACG8KQ_000413"
     CDS             complement(6036..6431)
                     /gene="dhaM"
                     /locus_tag="ACG8KQ_000413"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9103384.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6585..6905
                     /locus_tag="ACG8KQ_000414"
     CDS             6585..6905
                     /locus_tag="ACG8KQ_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9103385.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7017..7874)
                     /locus_tag="ACG8KQ_000415"
     CDS             complement(7017..7874)
                     /locus_tag="ACG8KQ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9103386.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7982..9286)
                     /locus_tag="ACG8KQ_000416"
     CDS             complement(7982..9286)
                     /locus_tag="ACG8KQ_000416"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9103387.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9283..9792)
                     /locus_tag="ACG8KQ_000417"
     CDS             complement(9283..9792)
                     /locus_tag="ACG8KQ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103388.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9967..12102)
                     /locus_tag="ACG8KQ_000418"
     CDS             complement(9967..12102)
                     /locus_tag="ACG8KQ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9103389.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12185..13840)
                     /locus_tag="ACG8KQ_000419"
     CDS             complement(12185..13840)
                     /locus_tag="ACG8KQ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9103390.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13842..14156)
                     /locus_tag="ACG8KQ_000420"
     CDS             complement(13842..14156)
                     /locus_tag="ACG8KQ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9103391.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14326..14586
                     /locus_tag="ACG8KQ_000421"
     CDS             14326..14586
                     /locus_tag="ACG8KQ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9103392.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14600..14914
                     /locus_tag="ACG8KQ_000422"
     CDS             14600..14914
                     /locus_tag="ACG8KQ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9103393.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15644..16198)
                     /locus_tag="ACG8KQ_000423"
     CDS             complement(15644..16198)
                     /locus_tag="ACG8KQ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9103394.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16324..18253)
                     /locus_tag="ACG8KQ_000424"
                     /pseudo
     CDS             complement(16324..18253)
                     /locus_tag="ACG8KQ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18559..19275)
                     /locus_tag="ACG8KQ_000425"
     CDS             complement(18559..19275)
                     /locus_tag="ACG8KQ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9103395.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19276..21099)
                     /gene="glpO"
                     /locus_tag="ACG8KQ_000426"
     CDS             complement(19276..21099)
                     /gene="glpO"
                     /locus_tag="ACG8KQ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9103396.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21113..22609)
                     /gene="glpK"
                     /locus_tag="ACG8KQ_000427"
     CDS             complement(21113..22609)
                     /gene="glpK"
                     /locus_tag="ACG8KQ_000427"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9103397.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22745..24205)
                     /locus_tag="ACG8KQ_000428"
     CDS             complement(22745..24205)
                     /locus_tag="ACG8KQ_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103398.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24195..25490)
                     /locus_tag="ACG8KQ_000429"
     CDS             complement(24195..25490)
                     /locus_tag="ACG8KQ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9103399.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25822..26499)
                     /locus_tag="ACG8KQ_000430"
     CDS             complement(25822..26499)
                     /locus_tag="ACG8KQ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9103400.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26529..27620)
                     /locus_tag="ACG8KQ_000431"
     CDS             complement(26529..27620)
                     /locus_tag="ACG8KQ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9103401.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27686..28156)
                     /locus_tag="ACG8KQ_000432"
     CDS             complement(27686..28156)
                     /locus_tag="ACG8KQ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9103402.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKKIKFFKQISCMYN"
     gene            complement(28740..30197)
                     /locus_tag="ACG8KQ_000433"
     CDS             complement(28740..30197)
                     /locus_tag="ACG8KQ_000433"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9103403.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30194..32089)
                     /locus_tag="ACG8KQ_000434"
     CDS             complement(30194..32089)
                     /locus_tag="ACG8KQ_000434"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9103404.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32147..33922)
                     /locus_tag="ACG8KQ_000435"
     CDS             complement(32147..33922)
                     /locus_tag="ACG8KQ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9103405.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33972..35468)
                     /locus_tag="ACG8KQ_000436"
     CDS             complement(33972..35468)
                     /locus_tag="ACG8KQ_000436"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9103406.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35662..37284)
                     /locus_tag="ACG8KQ_000437"
     CDS             complement(35662..37284)
                     /locus_tag="ACG8KQ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9103407.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37612..38925)
                     /locus_tag="ACG8KQ_000438"
     CDS             complement(37612..38925)
                     /locus_tag="ACG8KQ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9103408.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39170..39394)
                     /locus_tag="ACG8KQ_000439"
     CDS             complement(39170..39394)
                     /locus_tag="ACG8KQ_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103409.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39414..39836)
                     /locus_tag="ACG8KQ_000440"
     CDS             complement(39414..39836)
                     /locus_tag="ACG8KQ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9103410.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39978..40688)
                     /locus_tag="ACG8KQ_000441"
     CDS             complement(39978..40688)
                     /locus_tag="ACG8KQ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9103411.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40706..41203)
                     /locus_tag="ACG8KQ_000442"
     CDS             complement(40706..41203)
                     /locus_tag="ACG8KQ_000442"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9103412.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41240..41692)
                     /locus_tag="ACG8KQ_000443"
     CDS             complement(41240..41692)
                     /locus_tag="ACG8KQ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9103413.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41827..42432)
                     /locus_tag="ACG8KQ_000444"
     CDS             complement(41827..42432)
                     /locus_tag="ACG8KQ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9103414.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42882..43502)
                     /locus_tag="ACG8KQ_000445"
     CDS             complement(42882..43502)
                     /locus_tag="ACG8KQ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9103415.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43504..44373)
                     /locus_tag="ACG8KQ_000446"
     CDS             complement(43504..44373)
                     /locus_tag="ACG8KQ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9103416.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44396..44803)
                     /locus_tag="ACG8KQ_000447"
     CDS             complement(44396..44803)
                     /locus_tag="ACG8KQ_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9103417.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44822..46111)
                     /locus_tag="ACG8KQ_000448"
     CDS             complement(44822..46111)
                     /locus_tag="ACG8KQ_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9103418.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12944 a         9841 c         7892 g        15437 t
ORIGIN      
        1 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
       61 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
      121 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
      181 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
      241 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
      301 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
      361 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
      421 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
      481 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
      541 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
      601 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
      661 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac
      721 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
      781 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
      841 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
      901 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
      961 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
     1021 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
     1081 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
     1141 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
     1201 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
     1261 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
     1321 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
     1381 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
     1441 ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
     1501 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
     1561 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
     1621 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
     1681 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
     1741 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
     1801 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
     1861 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
     1921 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
     1981 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat
     2041 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
     2101 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
     2161 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
     2221 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
     2281 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
     2341 cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact
     2401 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
     2461 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
     2521 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
     2581 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
     2641 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
     2701 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
     2761 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
     2821 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
     2881 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
     2941 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
     3001 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
     3061 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
     3121 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
     3181 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
     3241 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
     3301 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
     3361 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
     3421 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
     3481 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
     3541 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
     3601 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
     3661 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
     3721 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
     3781 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
     3841 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
     3901 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
     3961 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
     4021 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
     4081 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
     4141 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
     4201 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
     4261 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
     4321 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
     4381 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
     4441 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
     4501 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
     4561 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
     4621 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
     4681 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
     4741 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
     4801 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
     4861 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
     4921 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
     4981 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
     5041 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
     5101 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
     5161 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
     5221 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
     5281 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
     5341 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
     5401 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
     5461 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
     5521 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
     5581 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
     5641 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
     5701 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
     5761 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
     5821 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
     5881 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
     5941 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
     6001 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
     6061 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
     6121 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
     6181 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
     6241 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
     6301 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
     6361 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
     6421 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
     6481 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
     6541 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
     6601 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
     6661 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
     6721 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
     6781 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
     6841 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
     6901 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
     6961 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
     7021 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
     7081 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
     7141 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
     7201 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
     7261 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
     7321 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
     7381 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
     7441 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
     7501 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
     7561 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
     7621 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
     7681 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
     7741 gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca
     7801 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
     7861 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
     7921 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
     7981 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
     8041 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
     8101 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
     8161 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
     8221 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
     8281 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
     8341 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
     8401 tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca
     8461 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
     8521 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
     8581 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
     8641 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
     8701 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
     8761 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
     8821 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
     8881 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
     8941 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
     9001 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatattcga
     9061 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
     9121 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
     9181 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca
     9241 aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc
     9301 attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga
     9361 ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc
     9421 aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt tatttacttt
     9481 tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca ggaaactgaa
     9541 aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg ataaaaaagc
     9601 gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga cccatataaa
     9661 aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa tctttaacca
     9721 gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt cttctcttgt
     9781 tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt taccatacat
     9841 ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa caaaacattt
     9901 ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt attttttatc
     9961 tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag atatcgccgt
    10021 gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat ataagattac
    10081 atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt tttctaatcc
    10141 atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg tttgggtgat
    10201 aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt tcaacaattc
    10261 ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga tcgatacgat
    10321 gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg ctccgctaga
    10381 acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt cgaaacaacg
    10441 cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt cacctactaa
    10501 agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca gggtataatc
    10561 ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt caacaagtaa
    10621 atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt gccaagcata
    10681 agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat ccacccatgc
    10741 catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt tccttgcata
    10801 agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct
    10861 atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag
    10921 atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt
    10981 aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt
    11041 tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg
    11101 ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc
    11161 tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga
    11221 aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt
    11281 cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat
    11341 cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc
    11401 acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt
    11461 ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa
    11521 gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg
    11581 atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg
    11641 aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg
    11701 ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa
    11761 ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt
    11821 caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg
    11881 atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact
    11941 gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt
    12001 tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt
    12061 cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt
    12121 ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg
    12181 agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg
    12241 caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc
    12301 tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc
    12361 ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt
    12421 cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat
    12481 gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt
    12541 ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat
    12601 cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac
    12661 cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact
    12721 tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa
    12781 tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg
    12841 cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa
    12901 ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa
    12961 taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa
    13021 tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt
    13081 cgattccagc ggtcactgct agctggttat caggacgaac accaatggat aaaataacta
    13141 aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc
    13201 cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa
    13261 aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga
    13321 cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa
    13381 cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta
    13441 catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg
    13501 gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat
    13561 gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca
    13621 catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt
    13681 cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc
    13741 ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag
    13801 acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt
    13861 cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc agatttcttt
    13921 aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt
    13981 tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc tccttttata
    14041 tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc
    14101 ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc
    14161 tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg agctttatta
    14221 atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg cataaaatac
    14281 cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat
    14341 aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg
    14401 atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt
    14461 attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct
    14521 gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa
    14581 aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc
    14641 agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt
    14701 atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga
    14761 tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga
    14821 aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact
    14881 gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat
    14941 cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca
    15001 aaggttgtga aaacgctgtc ctgcatcaag taataagaac agccaaacaa aaatagcttc
    15061 tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa
    15121 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
    15181 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    15241 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    15301 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
    15361 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
    15421 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15481 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
    15541 aacaacttct cctgtttttg gttcggttgg cttattatcg agaagtagct actgggagtc
    15601 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
    15661 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
    15721 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
    15781 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
    15841 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
    15901 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
    15961 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
    16021 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
    16081 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
    16141 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
    16201 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
    16261 caatgatttc actgttttta tttttcttta cagacactga tattatgagc taatttgaga
    16321 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
    16381 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
    16441 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
    16501 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
    16561 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
    16621 tatccgatca ccttttgcaa agagctgacg ttctttttta ttctccttcc tatttgtaac
    16681 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
    16741 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
    16801 gctttctcct gtttcttcca acccgcaact gatatttagg acgaagtgct tcccgatcga
    16861 aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg atttctccat
    16921 aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg attgcgatcg
    16981 ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa agtaccaacg
    17041 tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca gaaaatgtac
    17101 tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca tcaaagacag
    17161 caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc aaagtcaata
    17221 aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg ataatcaagc
    17281 ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt aaaaatggat
    17341 gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg cttccattga
    17401 tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc gtttcgatca
    17461 ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgcc aggacttgat
    17521 attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat ttagaaatca
    17581 cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc gttccatcta
    17641 ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt tgaacaatca
    17701 ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt aattttgact
    17761 ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt
    17821 cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca tttatcaaat
    17881 caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa
    17941 actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa tcggttggct
    18001 gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc aaccccataa
    18061 atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct tcttgacgat
    18121 ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt ttgctatagt
    18181 ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat tgactaatca
    18241 tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa taaataaggg
    18301 cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta tccttattag
    18361 aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa ataaactttg
    18421 tttattggct attcgactta ctgattagat accagctaca ctcacaaaac ataccccatt
    18481 cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg ttgtcaaact
    18541 ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga taattgctcc
    18601 gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc ctttattagc
    18661 tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt tgattgcata
    18721 tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta cagcgaatac
    18781 gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa
    18841 tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag gacctgttgc
    18901 aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca aataagaaag
    18961 ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga aaggaacgac
    19021 aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag gattcaaatg
    19081 agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc cccaaccaaa
    19141 tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca cagccgcaca
    19201 cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca tgatttgagt
    19261 catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt ctgcaatcaa
    19321 tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa aatatccagc
    19381 aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa acaacagatg
    19441 atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt ctaaaccata
    19501 acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt cttttgctaa
    19561 ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag catcttcttc
    19621 ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt ctaatttagt
    19681 tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt tcgtctctat
    19741 tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg ccatcttacg
    19801 gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc tttctaaaga
    19861 actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa
    19921 cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta ctgtatcgac
    19981 aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt aatcagaacc
    20041 agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat cattcaatgt
    20101 gatatttgct tctggatagc gatgattgat cacatccaat agataatcta catcttcttg
    20161 tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac caaagtaagt
    20221 cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg tatcgaagta
    20281 tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc ctttagttgg
    20341 acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat ctacccaagg
    20401 accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg tcagcaggtc
    20461 acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg ctttcatttt
    20521 acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca ctaaacgggc
    20581 atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt ttttcaaaaa
    20641 cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcatatt tggttcctgt
    20701 cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca tattgaaggt
    20761 tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg ggatgtgtgg
    20821 ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt ccacatcaaa
    20881 tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg aagtcccttc
    20941 tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg actggactgc
    21001 cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt cttgctgcat
    21061 tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct ccttattcaa
    21121 atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct cggcgctctt
    21181 cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc tcgtctgtat
    21241 ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca
    21301 agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc atcagatatt
    21361 cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca gtgtcatctt
    21421 gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta gctttaatga
    21481 tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc
    21541 aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct tcgcttgtag
    21601 agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt ccatctctca
    21661 gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag taaacctttc
    21721 catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc ggtttttcac
    21781 cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg cctggttcga
    21841 atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa attggtgctt
    21901 cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta gaagtgactt
    21961 ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc caatctagct
    22021 gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca tgtgcttgtc
    22081 catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat tcgccttttt
    22141 cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc gttgctgaga
    22201 aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt
    22261 ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca tgatagattg
    22321 gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt atgccaatcc
    22381 cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata actgactgta
    22441 cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg aaatattgcg
    22501 tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg
    22561 agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt gtgattccct
    22621 ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt aaaaaaagaa
    22681 actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg aagctatttt
    22741 ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga ataatgtcgt
    22801 aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc tggcaaaatg
    22861 tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata ccgccgattc
    22921 ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc agactttcta
    22981 aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa agaacattgg
    23041 catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc ggcaattgct
    23101 gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg agcattttct
    23161 gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca aagaaaaacc
    23221 attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg attttcaaac
    23281 gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa acagcggttg
    23341 gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct actatcgaaa
    23401 aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct gctgctgttc
    23461 gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc gactgataga
    23521 gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt ctttttttcg
    23581 taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata
    23641 cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa aaaagcggtc
    23701 gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc tgtagctctt
    23761 ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc tggttcaatt
    23821 ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct aatcgaacga
    23881 tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag ctttcctgca
    23941 ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc agcgcctgat
    24001 actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat tcaatatctt
    24061 ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa ccattgatag
    24121 aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac tgctcttttc
    24181 tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca caccatatct
    24241 ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc aaacgtccat
    24301 ccttcttcta ctgcaattgt cagttggtca agaaggtgga acatctcttt tttgctttgg
    24361 agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt aactttgctt
    24421 tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg gggataaaaa
    24481 atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca ctgattgccg
    24541 actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt gctgacattt
    24601 cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt tgttggacga
    24661 aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt ttgcaagtag
    24721 tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca catctgattg
    24781 tcgggacgag aatgattagc cagcaaaaca agatcatagt tctcctttct tttttccgtc
    24841 tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa
    24901 agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc tagataacga
    24961 aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga tgccaaccct
    25021 tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt tgcattcatg
    25081 atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat aagcgttgat
    25141 tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa
    25201 tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat tactgtcttt
    25261 tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat ccaagaaagt
    25321 tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg aatccatcct
    25381 aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg gcgaagataa
    25441 atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat ctgcattcct
    25501 ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta ttcataactc
    25561 gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat cataccgtgt
    25621 tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc atttctgaag
    25681 agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac gtacctctgt
    25741 ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta
    25801 gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt cgcctaattg
    25861 atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa ccacttctga
    25921 aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt ctgctaagaa
    25981 tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc cagtcactgt
    26041 acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc ctacaacacc
    26101 gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga ctttcccaag
    26161 atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat aaaccgctgc
    26221 ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc gatttctgaa
    26281 atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac tttgcggttc
    26341 taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat tttttactaa
    26401 tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct cgatcttttc
    26461 gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct ctttctataa
    26521 aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg caaataaagc
    26581 tctttatatg ctttttcaaa aggtttcgtt acaaattcat cctcacggtt ttccaataat
    26641 ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat
    26701 ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac taaagggatc
    26761 tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta tttatcagaa
    26821 cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt tgactcacgt
    26881 aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc atattcttct
    26941 ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac cgctgagaaa
    27001 accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc gccccattga
    27061 tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt atttgctcca
    27121 tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt gccaaatgct
    27181 tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc atcaggacgt
    27241 tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc tcgacttgtc
    27301 attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg gattgctttc
    27361 gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc acctggccag
    27421 ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag acctttagaa
    27481 cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt
    27541 gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg taaagtaatt
    27601 gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta acctatcgag
    27661 aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc ttaaaaaatt
    27721 ttattttttt ttgtaactta tctgttgcat cattcattgt ctgggctaat tctttacttg
    27781 cttcatttcc caaaactgct ttgtatgact gatgaagttc ttcccagcta ccatcgactt
    27841 ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa tgacaagcga ccttcgctaa
    27901 ctgttttgac gtatccttta tattctaatt tttcaacaaa acgagcaatc gttgatgggg
    27961 cggaatagag catttgtgca atatcttttt gtggtattcc atctttttct tttaatatca
    28021 ataaaataaa accataagtt ggtgccagac ctgtccttct aaacgcttca tcagctaatt
    28081 tattcaacat gcgatctagc tttttcacgc taaagaaaag acattcatct aacgcttttt
    28141 caattggctt ttccatttca tcccctccat tggcgtacat atcatagtat gatttttatt
    28201 ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc tagatttgaa aaaaagaagt
    28261 agccatcttc aaagaatttc ggcttgtttt cgacaaaact ccttctggaa caccgtgaat
    28321 cagaggttgt gaaaaagtcg tttagttttg agtatgaaga aaaattttgt gaaaatggct
    28381 ttccacattt ttgcataaat ttggcttaat accgaaaaac tggcttttga acaccgttta
    28441 tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata agaacagcca aacaaaaata
    28501 gttcctcata ttttgtcgtt ttttgagcta atgccgatgg tcagctgatt gtacaccgtt
    28561 tattgagagg tcgtgagcct gccgttgaac tccgagtcac aaggagcaat ttcaaaaaac
    28621 agcttctcat gttttatgac tatggtgact tgtgatcgag gagttggcag gagaatactg
    28681 tttattcgta aagaaggggc tgtgacaaga cttttgtcac agtcccttat ttccttttat
    28741 tataggattt cgccatttga ttggatgact tgtctgtacc aatcaaatga ttttttcttt
    28801 aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa catagatata tccataacgt
    28861 tttgacattt gtgaagtaga agcgctgacg atatcaatgc agccccacga cgtataacca
    28921 aataaatcta ctccatcttt gatagcttcc ttcatctgct caatatgctg cttcaaatag
    28981 tcaatgcggt aatcgtcttg aacggttcca tcttcttcta ctttgtctat agctccgagt
    29041 ccgttttcag ctatgaacag tggtttttga tacctatcat ataattgatt caaagcaatt
    29101 ctcaaaccct taggatcaat ctgccagccc cattcacttg atggaagata cggattttta
    29161 attcctccag ttattaagtt tccttggacc ccttcccaat tttctggatg agcactgact
    29221 gcactagaca tgtagtagct aaaagaaaca aaatcaacag ggtaggctgc taagatttct
    29281 tcatcaccta cttctatttt taagttgaca cctttttctt caaacattcg ttgcgtatac
    29341 gctggataat atcctcttgc ttgaacatca ctaaagaaca aagaagttct ttgctgctca
    29401 attgtttttt gaatatcatc cggatcacaa gaatacggaa caagaagctg atagctgatc
    29461 atacaaccga cttgtgaacc tttgataatt tcatgacaag cttttgtagc taaagcactt
    29521 gctataaact gatggtgtgc accttggtat ttcgcttgtt ccaaatgggg atggttttct
    29581 tcaattactc ctacgctaac aaaagggtga tgttttacac agttgatctc attgaaagtc
    29641 atccaatatt ttactttatt tttatagcgt ttgaatactg tttcagcgaa acgaacgtaa
    29701 aactgaataa gctttcgatt aggccatcca ccatattctg taaccaaata taacggcatt
    29761 tcataatggg aaagagaaac gactggctca atcccatatt ttagacattc gtcaaacact
    29821 tgatcataaa aaaataatcc cgcttcattg ggtgtttctt catcaccatg aggaaaaatt
    29881 cttgtccatg cgatagacat ccgatagcat ttaaaaccca tttcagcaaa taaacggatg
    29941 tcttctttaa agtgatgata gaaatcattt ccgtgtcttt taggataata atacttgctg
    30001 ttgggatcct gcgccgcttt tacttgctga tgcgtcatca tatgccattg atctgtccat
    30061 ttttctttag gtaaatcgct gtcgaatgta tacacatcag acacagataa tcctttttca
    30121 tctacatccc atgcaccttc acattgattg gccgctgtcg ctccgcccca taaaaatcct
    30181 tctggaaatt ttttcatatc gatcccccta aacttgctag ttttcttgtt tgattatttt
    30241 tcttatgact agtaatgata tttttgaatc tactactatt tgtaacaata accattgtgg
    30301 tcggatcata tccctctttg attaattctt gcaaatctac ttccatcaat aaatcgcctt
    30361 tacgtacttt ttttccatca gatactgcac tttgaaagta tttgccattt aaattcactg
    30421 tatcaatacc gatatggatc agtatctctg ctccttcttc actgactacc ccaattgcat
    30481 gtttcgttgg aaaaactgtt gaaacttccc cgtttacaga actgtagatt tttccgtccg
    30541 ttggaatgat cgcaaaccct tctcctaaag atccactagc aaatgttgga tcgtttactt
    30601 cagataaatc aattatttca cctgctgcag gagtacttaa cactatatcc tttgaggatt
    30661 cttcttcgat ttcatccttg tagccgaaaa gaaaagttag catacaggaa ctgatcatcg
    30721 ctaagaaata actagcaact agccaaacaa atccttcacc aatataaatc gggaaagaca
    30781 aaatacttct tgtagcaact gccaaagcac cggctcctgc ggcaccagct actccaccaa
    30841 atatagctcc acatatacaa gcaatgtaaa atggtcgttt atatttaaga gtcacaccat
    30901 aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa tgcagataaa gataaacttt
    30961 ttactttctt atttttcgtt ttcaaaaata ccccaagtac tgctcctgct tgcgcagcta
    31021 cggcagtcat acatgctgga cctagcgtat cacgaccgta gaaagaaaga ttgttggtca
    31081 ttactggtac aagtccccaa tgtaacccaa aaatcactaa cacttgccaa aatccgccga
    31141 taacagcacc agataaaaca gggctaaagt tataaacagc taaatatcca gctgcaatca
    31201 gctgacttgc ataatctgca attggaccta caactaataa aataattggg accatcacga
    31261 tgatacaaat gaatggtgtc aataaatttc gaatactttc gtgcaagctt ttgcgaatct
    31321 taggctcaat ataagaaaga acccatacag ctaaaattgc tggaataacc gtacttgtat
    31381 accttgctag aatcacgggc atccctagaa atcgcaaggt tatgctatca ctatatgcac
    31441 tgacaatcgt aggataaacg agagctgctg caataaccat agcgatgaaa cgatctgtat
    31501 tgaacttttt tgcagcagta tatgccaaaa atataggcaa gaaatagaaa aatgcatcag
    31561 ctgctgcatt caaaatgata taagttcctg attcagtagt taataaattc aaactcgtac
    31621 ataaggcgag tactcctttg atcaaaccac ttcctgccaa gacgcctaaa gtcggggcaa
    31681 aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc ttttttctca gttcctctct
    31741 ttccactaga tgcatcatct tctagtttca tttgtttgga taatacgtca tacacttcat
    31801 ttactgcatt tcctactacg acttgaaaca ttcctccagc ttcgactaca gtgatcacac
    31861 cttctagttg ctctaccttc tccttatctg ccttcttatt atcttttagg gtaaagcgta
    31921 accttgttgc acaatggtaa acattgctga cattttcttc gcctccgaca aatcggacaa
    31981 tatctttcgc taattgtgaa tagtccattt ttacttcccc cttgttaatt aatgccgtag
    32041 caatccttct tacaagataa gtatagttct tagaaaacgg tttctccatt caattagtac
    32101 cccttctaat agcgaacgaa actgtacaat agtgtgtatg gtttacttag ccgagcaaat
    32161 cactcactgc ttgaaagaaa acaagtatat cgtttctctt atagtcaaag gagacaaaaa
    32221 aaacacgctt gatcttcctt tgctgatgta taagtggtat ctcaagctga tactctgtga
    32281 tttttgtttc tacttggctt ttgataagac agcacaacaa agtctcggca tttaacggaa
    32341 gcataactag gtcagaacta ggagagatta gttgctgaat ggaatctatc tttttatgga
    32401 tttttatttg attttcttct ttgtagtaat gatcaagcag ttttgttttc tttttttctt
    32461 caactatggt cagcttttca cgcaaaatct gataaatctc ccaagattca aactcatcta
    32521 gaacactagg tagataaatc aaatcatccc actcaaaaat aagctcttgc tcagtagtca
    32581 aaattgcatc atattccgtt attttgtttg attgattttt gaaaagatac actggttcta
    32641 tccggcattc tttgatccac atgttcatac tttgttgaat ctgttttttt agtgtattca
    32701 aaatactaat tggctggtta ctaacaatca gtatctcccc ttgttttcga aaaggattca
    32761 acgcttcggc aagatacaaa acaaaataag cgctttcagc taaattggga cgtaattgaa
    32821 aatgtattgg gaagaaacgc cgaattaaat aaatttctaa aggataacgg gagatgatct
    32881 tttgtacaaa atgattcatg gtattatgtc cggcatccat cctcgttttc atttgtttga
    32941 tatgggttag caaaaattct gtttcatgga acaataacga aacaggaaga tctaaaaaac
    33001 gaataatcgt ttgctcaaat gctcttagat tcttctctat ttctttgttt ttttcatcat
    33061 taatcatcaa ataatttaac tctaatagtc gattttcaat tgcttgtttt tcttgattgg
    33121 aaaaagaata gggtagttct gatgataact tttttgtgag ggtctctacc atcggaataa
    33181 ataatgtata cctgtttgtt ttttctaaaa gttcttctct tcgaagaatg gtcaaaacga
    33241 gataccgaga aaatcgtatg aagtcctctc ctgaaacaat ataattatat gatataagaa
    33301 cctcttttaa tgtcttttgg acagtcggca tgatattgga aaaaattgta ctgatttgtt
    33361 gaggaaacct tgcgtgttgg atcaccgttt ctgttccaac aagagataag aaaattcttt
    33421 ttgaattttc ttcagcaatg atttttagtc cttttgtcga cgaaacttcc agttctaatt
    33481 taggattgcg ctcgatccaa cgcaaaatcg tttttatttg taatgatacg gctgtttttg
    33541 aaagaaagaa aatgtctgct atcttttgca tactgacata ccctttttga aacaataagt
    33601 acgtcacaat agttgaagct cggaaatcca tgctggaata gattttatgt ttcgtcacct
    33661 cttgtatgat ttgctgttta gtttcagatt caattgattc taaaaaataa cctgaacgtt
    33721 tatttgactg gataatgatt ttcttctcgt agatagcaag catatttttg atttcagttt
    33781 ggatcgtttt cttgttggta cctaaagctt cagctaattc ttcgcttgtt atccaatgat
    33841 tcttatttct taatactaca agaatttgga gttgtcgttt atttaagtcc atttgcttac
    33901 acctcctctt tgttataaac atacaggaaa aacctctcaa atgacaatag tcacttgcag
    33961 aggtttaaaa atcatttgaa gaaagatcta tattcaaggt ttttaccatt agactggata
    34021 atttgtttat aatattcaaa actatctttt ttcaatctcc taaagcttcc gctcccatca
    34081 ttttgtctat ccacataaat aaagccatat cgcttcctca tttcccctgt tcctgccgat
    34141 acgatatcga ttggtcccca ccataagtaa ccaatgattt ggcaaccatc ttttattgcc
    34201 tcagcaattt cctgaagatg tatttttaga tactcacaac gttttggatc gtggattcga
    34261 ttcaattcat ctggtgcttc atctagtcca atgccatttt caactatgaa taatggtaat
    34321 tcataacggt ctgtcaattc attcatgaca taacgaaacc ctagaggatc aattggccaa
    34381 ctccaaggtt cgggagattt ttccttcaaa aagggattat cgaaaccatt tgctccgcca
    34441 gtatctacgc gcatttctgc ttctttttta tagacagcgc tacgataata actaaatgca
    34501 atataatctg ccgtgttagc ttgcaagcac tgttcgtctc catctaacat tactggtgca
    34561 cagtcttctt ccttccatgt cctgtaaata taagctggat aatgccctcg gcacatgaca
    34621 tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac caaatacatt ctctggatca
    34681 cagtcatatg cataagtcgc taatgcagaa aagctacaca taatacccat cttagcattt
    34741 ggatcgattt tctttaataa ttgagtagca cgggcgttcg ctagaaacat atgatgacag
    34801 gcttgataaa tcactttttt tgttagtgta gcaatgggtt caagtttatc tctagcaggg
    34861 aatgtaacca atccagcagc tgcataaggc atacgaaaag cattattgat ctcattaaag
    34921 gtcatccaat atttcacttt cccttggtag cgttcaaaga cggtctgcgc ataaaattca
    34981 aaaaagtcga ttaattttcg attttgccac cccccatact ctcctaccaa tgctaagggg
    35041 gtttcataat gactaagagt aattactggc tcgatatcat atcgatttaa tgtattgatt
    35101 agtctgtcat aaaatacgag accagcctca tttggcttct gttcgtctcc tcttggaaat
    35161 attcgtgccc atgagatgct tgttcgaaaa gcttttaaac ctaactcctt cagtaaacga
    35221 atatcctctt caaatcgatg ataaaaatca atcgcttcat gagaaagata atggagtgat
    35281 tcgtctaaag caatctccca aatctttttt tcttcattcc actgaatact aggatgctgg
    35341 ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt tgccgtcttc caaccatgca
    35401 ccttcacatt gattagcagc tactgctccg ccccataaaa aattttctgg aaatatttcc
    35461 tgatccataa ctatctctcc tcgctatcaa caatttctta cttgaagtat agctcgattc
    35521 atcaaaacaa gatattctaa ctatttcggc agaagagaag aaaaaaattg aatgctactc
    35581 ttcattttta atatttatat ataacaaata cataagacga tccccgaaag aaaacactcg
    35641 ttaaccatct aagcaaacga tttattttac ttccaacaca aatgcttcat aaggtcgtaa
    35701 atcaactttg ccgtctggta actgtttcag aggataattt gacacaataa cccttaaagc
    35761 ttcacgtgga atagtgaacg tctgcttttc actggacata ttacaaataa cgagccattg
    35821 ctggtcttca tatatgcgag aataggcaaa gacatgctca ggcgtctttt ccactaattc
    35881 atagtctccc cagacgatga tgtcatgttt tctacgcaaa tcgatcaatt tcttgtacat
    35941 ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca ttgatttctt tataatttgg
    36001 attgactcga agccatggaa ttccagtagt aaacccagca tgttcacggg cgttccattg
    36061 cataggcgtc ctagcattgt ctcttccttt cgccttgatt gattccaaga tttcctcttt
    36121 tgtaaatcca gaagaaatcc tttcgttata catattgatt gtctcaatat cttttgcttc
    36181 cgcgatatca tcgattgggc aattcgtcat gcctaactct tctccttgat agatataagg
    36241 ggtgcctttc atcatatgga gcaagatagc aaacatcttg gcactttctt ttcgatacac
    36301 atcgtcattt ccccaacgag aaacaattcg cggaagatcg tgattattcc aaaataaact
    36361 attccatcct tcatcaccta aagatgtttg ccatttggat aatacttttt tcagttcaga
    36421 gatactcaag ggtttcaaat cccatttgtc tttgccttct tgctgatcta atcctatgtg
    36481 ttcgaattgg aagaccatgg ataattcatt tcttttagga ttggaataaa gtttagcgat
    36541 ttcggtagtt gctccccatg tttcgccaac agtcatcaga tttttgtcac caaaagttgc
    36601 ttgattcatt tcctgaagat actcatgcag tttaggtcca ttacttgtga tttctttatc
    36661 cggttctttg ccaatcatat cgatcacgtc catacgaaag cccccaatac ctttatcgat
    36721 ccagaaattc atcatttgat aaatctcttg gcgaacctgt ttatttttcc aattcaaatc
    36781 aggttgacgc ttgctataca agtgcagaaa atattgcccc gttttttcat ccaactgcca
    36841 agcagaacca ctgaatattg agcgcaatct attaggctct tttccattga ccggatcacg
    36901 ccaaatataa tagtcccgat aaggattttc tttattttta cgggcctcaa tgaaccacgg
    36961 atgttcatca gaagtatgat tcaccaccag atccatgatg atacggatat ttcttttctt
    37021 tgcttcttca atcagttctt ccatttcttc catcgtaccg aattctgcca tgattgcttg
    37081 ataattactg atatcatacc cattgtcatc attcggagac tcgtatactg gagaaagcca
    37141 aattgcatca atacctaaat acgccagata atccaacctg gaaataatcc cccgcaaatc
    37201 tccaatacca tcttgatttg aatcttgaaa acttcttgga tagatttgat aaacaactga
    37261 atggtgccac catttttgtt ccatgatttt cctcctataa atggatgttt ctattttttg
    37321 cttaatcatt tattttagtc tctctcacta aaaattgtaa cgctttcaaa tagctattgc
    37381 accttgttat cggtaacaga ataaaacaaa aaaacagctg aggaaattcc ctcaactgtt
    37441 ttttgaaagg attgatttat gcagggaaga atgctgattt cacaacttct ttataaacgg
    37501 tgttcactca gctgtccctt ggtattagct caaaaaacgg caaaatataa gaaactattt
    37561 tttcgttttt ttctctaata ctcaggccag aacgctgatt tcacaacctc tttatttcac
    37621 attcaatatc cacatgaatc ccacaaatac aaagaacaga ccatacatga ttggagatac
    37681 ttcttttcct cttttagctg ctaacatggt aattggatag aagataaaac ctaatgcaat
    37741 cccatctgaa atactataag tcaaaggcat gccgatcaag atcaagaatg aagggatcgc
    37801 aatctctaag tttccccatt cgatttgtcg caaagattgt gccatcaata ctcctaccat
    37861 aatcaatgct ggtgcagtta cttgtgaagt aacaactgat agtaatggag aaaagaacaa
    37921 gcctaacaag aagaaaatcc ctgtagtaat agctgtgata cctgaacgtc ctccaacggc
    37981 gataccagct gatgattcta caaatgcacc aacaggagaa gtaccaaata aggaacccgc
    38041 aagcatagct gttgaatcag aagctaaagc tttccctaca cgaggcatct tgttgtcttt
    38101 catgaaacct gcttggtttg ctaatccaac taaagttcct gctgtatcaa agaaagtcac
    38161 aagcaagaat gtcagcacta cgacccacat ttgaagagaa ttgatgtccc ctacatgttt
    38221 taatgcaact aaaaatgttg gtttcaaact tggtgctgcg gcaacaattt cagaaggcat
    38281 tggaatcaaa cctgtgacca gacctaaaat cgttgtagct gccatcccga tgaagatgcc
    38341 acctggtaca cgccgaacaa gcaagatagc agttacgact aatccaaaaa tcgtcaacca
    38401 agtagaacca acgttgagtg atcctaatcc tactaatgta gattcattcg aaacgatgat
    38461 cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg ataccgccag aaatcgcata
    38521 tttcaaatct gctgggatcg catcaatgat catttcccgc agcttgaaga ttgtgatcaa
    38581 aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct gtttcccatg gaatccccat
    38641 tcctaaacaa acagaataag caaaaaaagc attgatccca agagcaggtg ccgttccaat
    38701 tggataacgg gctaataccc ccattaaaac acagccgatc gcactggcta atgcagtcgc
    38761 agtaaacact gctccttcat ccattccaga agcacctaat acagttggat ttacaaataa
    38821 gatataagcc attgagataa atgttgtaaa tcctgccagt acctcacgtt ttaccgtggt
    38881 gttcaatttt tctatttcga agtaggaaag tattttttcc ttcatcttcc atccccctaa
    38941 atccgaacat tataaataaa gtccgttaaa atttcgattt attaataaaa caacgtgttt
    39001 tatcatatac taaaacaaaa cataagtaaa caaaaaattc ggattttaag caaaatccga
    39061 acgttccatc tgattaaatc cctttatctc aaagttcatg aacattcact ttggattaca
    39121 agaatttctt tattgtgtta ataaagtttc ttcattctag tctcaaaaat caatagtgtg
    39181 atttgatttt accatttcgt ttctgttctt ctttgatgaa ttcttttgta tcgacaggat
    39241 tcagattatt tagtccgcca aaacctgtat cgaccatttt tccccagcct tttgtcttct
    39301 tttttcctga tttgagattc tttgctcgaa gcttctcact atgttttgct cgccgatact
    39361 tcaaataacc ttctaatctt cccattagat ccactgtttt tcctcctttt tattcaatca
    39421 gacgactcag cttttcctat aacaaaataa ttgccttcag ggtcagaaaa attgaaagtc
    39481 aagatgccat tgttatctgt gatttctcct ggttcttcca tgttctcgta gatagtttca
    39541 aattcatccg taaaaaatac taaagaaggc ggtggtccta atacctctgg tgaaaacttc
    39601 tgcacaaata cttttgggaa tacgcctaat tctaattctt ttgagatgct gagtacaata
    39661 ctcttaaatt cttctggcaa ttcgattgtt tcagaaattt ctgcaccaat tttttctaca
    39721 aagaatcggc aaatcatctc tacgtcattg acataaagca tcacacgtgt tttttgtatc
    39781 atcatttgac ccttctttct atttgatttt ctttcctgtc tataaactga ttccattata
    39841 tcacgaaagc gcaaacacct tgtgtttgat actattcaag aaaaataaag caatagtttc
    39901 taggattgct ttcctccaca aacttttttt acaaaaaaaa aacggcaatg aatgccgctc
    39961 ttcttatttc gattctttta taattctgct tctgattcat ctttggttag tgtgtgtcta
    40021 acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg
    40081 ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc ttgtgtttct
    40141 tccgtcagca tcgttctttg ggcttcacgc cagttgagac agcgacagat cgctccttgt
    40201 tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc acctaaaggc
    40261 aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat cttttcgata
    40321 tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat atctacaagc
    40381 ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa
    40441 gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg agcaatgact
    40501 tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc tgcctcattc
    40561 aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat accatttacg
    40621 gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc cactattact
    40681 tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga tagtctgttt
    40741 ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct tttttacgat
    40801 aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct gttttttgct
    40861 tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct tgatgtttaa
    40921 atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt tcctcagatt
    40981 gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata ctcactagga
    41041 aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca taagcttgtg
    41101 tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct gtaaatgttt
    41161 cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga atttcgtatt
    41221 tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca aaattccaat
    41281 cttcgctaac attcttgcat actttattta aaagatattc caattgtttt gcttcttcgt
    41341 cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg atgaaaggat
    41401 agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt tctttatctt
    41461 ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca gtcgttgtac
    41521 gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt tcgcaaatac
    41581 gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg atattgctaa
    41641 tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc atttcatccc
    41701 acctttattt tgtttgtaag actagttcac ctctttttgt tgcaaatgca actattttca
    41761 ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct tcttttacga
    41821 aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact gtttcttcgt
    41881 tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca tgttggaagt
    41941 ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa aaacaagttt
    42001 ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa
    42061 ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca cattttcttc
    42121 aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg tccgtttatc
    42181 ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta cttttggtgt
    42241 tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt cttttcctat
    42301 ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt tatcaaatac
    42361 atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt attgagccaa
    42421 cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta atcaagtata
    42481 gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat aaaagaatgt
    42541 taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa atggcgttca
    42601 aaagcttgca tctgcggtaa taagccccac caaacgaaaa atatgacgag ctattcttgt
    42661 ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca ctaaacggtg
    42721 ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag aagctgtttt
    42781 tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc tcggtaaaca
    42841 gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt gtcattgatt
    42901 tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc tgttacatca
    42961 aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat gatctttttg
    43021 aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac gtcaccatag
    43081 tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg tttgtaccga
    43141 cgttgccata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg atatccgtat
    43201 ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata ctcggacaca
    43261 atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa aggataaact
    43321 ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga ttctgcatag
    43381 tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc ccgtagttta
    43441 ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa atactttttc
    43501 atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc caatcttatg
    43561 cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa gagacttgtt
    43621 gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta aaagctccgt
    43681 tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt cttctttcac
    43741 acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt ctttcagcaa
    43801 gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc
    43861 taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc ttgtatatac
    43921 cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc caagttctcc
    43981 ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct tcaggatatt
    44041 ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc tgtcgattgc
    44101 ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat caaccatttc
    44161 ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat atttagcggt
    44221 atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc ctagcatagc
    44281 aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca actcttctgc
    44341 tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta tctttttatt
    44401 tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt
    44461 agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc tggttataga
    44521 ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa ctcttttcta
    44581 aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca acttgttcat
    44641 aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa
    44701 tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct tttgccagaa
    44761 cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc cctactttca
    44821 attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct gcggctactg
    44881 tatctacatc ggttgctgta gcattgttga atcccatcga attaaagatc gttactagca
    44941 aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc gctcctcggc
    45001 gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa ttggttagca
    45061 taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca ataatcgtac
    45121 caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga aagaaaactg
    45181 gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct cgaaatgccg
    45241 gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca cccactacga
    45301 attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat aattcagaag
    45361 cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc acgaccatga
    45421 cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata tcttcgatcg
    45481 actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca aataagtacc
    45541 cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta cgaatgatcg
    45601 gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca ccgaccaaga
    45661 tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc
    45721 ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga gccaaaataa
    45781 tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa cccaaatcat
    45841 tcattgcttg accattgata gattcaatag aagggatgat cccttgcata tgaaatcctt
    45901 cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa ccggcactca
    45961 acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct gctgatttct
    46021 tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa
    46081 tattttcgat gataaaattg ataacgccca tgtt
//
LOCUS       DAZKCE010000009        44673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000009 DAZKCE010000000
VERSION     DAZKCE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.009"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1034)
                     /locus_tag="ACG8KQ_000449"
     CDS             complement(<1..1034)
                     /locus_tag="ACG8KQ_000449"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9103419.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINI"
     gene            complement(1311..3113)
                     /locus_tag="ACG8KQ_000450"
     CDS             complement(1311..3113)
                     /locus_tag="ACG8KQ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103420.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3127..3879)
                     /locus_tag="ACG8KQ_000451"
     CDS             complement(3127..3879)
                     /locus_tag="ACG8KQ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103421.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3888..4250)
                     /locus_tag="ACG8KQ_000452"
     CDS             complement(3888..4250)
                     /locus_tag="ACG8KQ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9103422.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4426..5298)
                     /locus_tag="ACG8KQ_000453"
     CDS             complement(4426..5298)
                     /locus_tag="ACG8KQ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9103423.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5349..5837)
                     /locus_tag="ACG8KQ_000454"
     CDS             complement(5349..5837)
                     /locus_tag="ACG8KQ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9103424.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(5975..6766)
                     /locus_tag="ACG8KQ_000455"
     CDS             complement(5975..6766)
                     /locus_tag="ACG8KQ_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9103425.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6821..7429)
                     /locus_tag="ACG8KQ_000456"
     CDS             complement(6821..7429)
                     /locus_tag="ACG8KQ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9103426.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7422..7739)
                     /locus_tag="ACG8KQ_000457"
     CDS             complement(7422..7739)
                     /locus_tag="ACG8KQ_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9103427.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7895..8623)
                     /locus_tag="ACG8KQ_000458"
     CDS             complement(7895..8623)
                     /locus_tag="ACG8KQ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9103428.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8782..9663
                     /locus_tag="ACG8KQ_000459"
     CDS             8782..9663
                     /locus_tag="ACG8KQ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9103429.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9900..10943)
                     /locus_tag="ACG8KQ_000460"
     CDS             complement(9900..10943)
                     /locus_tag="ACG8KQ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9103430.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10946..11614)
                     /locus_tag="ACG8KQ_000461"
     CDS             complement(10946..11614)
                     /locus_tag="ACG8KQ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9103431.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11756..14092
                     /locus_tag="ACG8KQ_000462"
     CDS             11756..14092
                     /locus_tag="ACG8KQ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9103432.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14207..14518)
                     /locus_tag="ACG8KQ_000463"
     CDS             complement(14207..14518)
                     /locus_tag="ACG8KQ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103433.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14699..16174
                     /locus_tag="ACG8KQ_000464"
     CDS             14699..16174
                     /locus_tag="ACG8KQ_000464"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9103434.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16221..16805
                     /locus_tag="ACG8KQ_000465"
     CDS             16221..16805
                     /locus_tag="ACG8KQ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9103435.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16807..17226
                     /locus_tag="ACG8KQ_000466"
     CDS             16807..17226
                     /locus_tag="ACG8KQ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9103436.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17507..17791)
                     /locus_tag="ACG8KQ_000467"
     CDS             complement(17507..17791)
                     /locus_tag="ACG8KQ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9103437.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18143..18853)
                     /locus_tag="ACG8KQ_000468"
     CDS             complement(18143..18853)
                     /locus_tag="ACG8KQ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9103438.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18850..20322)
                     /locus_tag="ACG8KQ_000469"
     CDS             complement(18850..20322)
                     /locus_tag="ACG8KQ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9103439.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20460..20933
                     /locus_tag="ACG8KQ_000470"
     CDS             20460..20933
                     /locus_tag="ACG8KQ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9103440.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21086..21637)
                     /locus_tag="ACG8KQ_000471"
     CDS             complement(21086..21637)
                     /locus_tag="ACG8KQ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9103441.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21742..22272
                     /locus_tag="ACG8KQ_000472"
     CDS             21742..22272
                     /locus_tag="ACG8KQ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9103442.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22755..23198)
                     /locus_tag="ACG8KQ_000473"
     CDS             complement(22755..23198)
                     /locus_tag="ACG8KQ_000473"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9103443.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23214..24374)
                     /locus_tag="ACG8KQ_000474"
     CDS             complement(23214..24374)
                     /locus_tag="ACG8KQ_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9103444.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24881..26581
                     /locus_tag="ACG8KQ_000475"
     CDS             24881..26581
                     /locus_tag="ACG8KQ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103445.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26683..27516)
                     /locus_tag="ACG8KQ_000476"
     CDS             complement(26683..27516)
                     /locus_tag="ACG8KQ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9103446.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27820..29985)
                     /locus_tag="ACG8KQ_000477"
     CDS             complement(27820..29985)
                     /locus_tag="ACG8KQ_000477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9103447.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30245..32539
                     /locus_tag="ACG8KQ_000478"
     CDS             30245..32539
                     /locus_tag="ACG8KQ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9103448.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32532..33197
                     /gene="pgmB"
                     /locus_tag="ACG8KQ_000479"
     CDS             32532..33197
                     /gene="pgmB"
                     /locus_tag="ACG8KQ_000479"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9103449.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33190..34197
                     /locus_tag="ACG8KQ_000480"
     CDS             33190..34197
                     /locus_tag="ACG8KQ_000480"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9103450.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34219..35238
                     /locus_tag="ACG8KQ_000481"
     CDS             34219..35238
                     /locus_tag="ACG8KQ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103451.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35519..36886)
                     /gene="nhaC"
                     /locus_tag="ACG8KQ_000482"
     CDS             complement(35519..36886)
                     /gene="nhaC"
                     /locus_tag="ACG8KQ_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9103452.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(36995..38416)
                     /gene="tyrP"
                     /locus_tag="ACG8KQ_000483"
     CDS             complement(36995..38416)
                     /gene="tyrP"
                     /locus_tag="ACG8KQ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9103453.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38659..40536)
                     /gene="tdc"
                     /locus_tag="ACG8KQ_000484"
     CDS             complement(38659..40536)
                     /gene="tdc"
                     /locus_tag="ACG8KQ_000484"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9103454.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40827..42083)
                     /gene="tyrS"
                     /locus_tag="ACG8KQ_000485"
     CDS             complement(40827..42083)
                     /gene="tyrS"
                     /locus_tag="ACG8KQ_000485"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9103455.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42195..42400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42663..43964
                     /locus_tag="ACG8KQ_000486"
     CDS             42663..43964
                     /locus_tag="ACG8KQ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9103456.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44033..44632)
                     /locus_tag="ACG8KQ_000487"
     CDS             complement(44033..44632)
                     /locus_tag="ACG8KQ_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9103457.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        13603 a         9219 c         7905 g        13946 t
ORIGIN      
        1 tttatattta ttttgtattc cgttacaaac tcgcgtactt ggttaggttc cagagtgaca
       61 tctccaaaac tttcaagtcg ctccgctcct ggcagttctt gagtctcaaa tgtgataccg
      121 ccatgattta ctaatttatt cccgcgcatt tccggtccat cttctccaaa atttgctgta
      181 aaaatcacga ttcctgaagc atctgttttt acttggatcg atactttttc atctgggctt
      241 gtcaattccg cagcatttag taaaccagat tgttttttca acaaaaatgg atgatcgatg
      301 ccgcccacta tcattgattg gcgaaaatca ctggcaaaga cctcacctaa ttttttagga
      361 cgttggaagt caaatgctgt gccacttact gaaactactt ctcctgtagg cgttgtatct
      421 ttattgagcg tagcaaataa tcggctattc aaccaaagta tatgttcgtc gattgactgt
      481 gctggatcac cggttaaatt gaaatagaca tgatttgtag gattgaatac tgttttgcgg
      541 tcactgatcc cttggaccgt cactcgccat gtatcatcgt tcgtcagcgt ataccgtact
      601 tctacatcta aagttccagg aaatccgttt tccatatctg ggctagtctg atagaaaatg
      661 accgatgctt cgttttcacc attgatgact gtataatgcc actgcttggc ttcaaagcta
      721 ggtgcaccac catgtaaaca atgaccagtg tccgggtctg tttttacttc gaacgtttca
      781 tcatctaatt tgaatgtgcc attcgtaatc cgtccagcca tacgtccgat tgaagcaccg
      841 atataagggt ctttttctag atattctttt gcttcatcaa aacctaagat caactctcgt
      901 ttttcattat cgattattgt ttgaaaatga acaacccgtg ccccaagatc actgacagac
      961 aaagatgtcc cattttgatt cgtgatcgta atcaagtgat agttttcgcc aaaattttgt
     1021 tcattgattt ccatgttcct tgcaccctct tttctttcat ttgcactgat tccagtatac
     1081 atggaaaccg ttttcttaac agcttattcc gtgagtaacg agaaaaaatt tactatattc
     1141 taataaaaga ccgtgaaaaa aaggttgtga caaaaatatt gtcacaacct ctttttccga
     1201 acaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga
     1261 ggagctattt ttgcttggct gttcttatta cttgatgcag gacagctttt tcacaatctc
     1321 tttttcaata cgattccagt atataaccat gtcgtcagta catagtagat gaaatacaat
     1381 acgaagaata tcgtaaacgg aacccaaata tctttgtatg gattttgcat gaatagacca
     1441 aacatttgta atccgaataa gacatggact acacctaaga ttcctggtgc taagaataac
     1501 acgcctactt ctctacggat cgattgttta agcaacccgc gtcgtgttcc tatcttttca
     1561 agcatttcat aacgagcaat gtcactcttg cttcctgaca agatcttgaa catcaaacag
     1621 ctagcaagca tggtcaagaa agctaaacct aggaagaatc ccataaattc aaaaccagag
     1681 aatagaccat tatacacttc atacatttca tatttctggt tgctgtactc tactttttgc
     1741 aaagatggat tgttcatttt attttgagca acaagtgttt cgattggttt caggtttgct
     1801 tggaagtctt ttacctgaac tagctgtaaa gtggattgtg gcaagttcag ttgatcaaat
     1861 tctgattgac tgactacctt gatttctttt tcttgctgtt ccggcaattc atagcttcga
     1921 agttcctctt gtacagtcag ttcattcatt tttttagcag gaactttttc tttcgtgatc
     1981 ttcggcggtg ttccatcttc atgttttatc atgatcaatg gttgagccgc aaattcactt
     2041 gcattatagt aaatcgttcc cgcatcttct ttttgagtat aagaagcaga aagtgttggt
     2101 gaaagttctt tgattttttg ctgatcttct gcccgaggat tattcatcac tagatcatat
     2161 ccagaattgt tttctgtcaa gtctgcaatt tcatttttaa aacctaatcc taccgtcaaa
     2221 gcgcccagtg ccaaggcaaa cagcatagcg accattgaga gcatttgtgt atattcacga
     2281 atacgaaagt ttaactgtga caatgtgaaa ttgttcaatt ttttcaatga aatctgttct
     2341 gatttcttta gtaatgataa aaagaaaatg atcacagaat ggaaaatgaa ataagttcct
     2401 aatacgatcg taactaaggc tacccctata ccaaagactt gataggtccc cacgtgtttc
     2461 atcatatagt aaccgcagcc tagaaaagca attcccagta ctacttcaag gaaaaacaag
     2521 aatttatgct gcttgattct tgttggtgtc tgatccgcac gaatcaatgc taaaatcggt
     2581 tttttgacaa tcgaaaaagc attgacaaca gcagctaata aaaacaaaac agcaaaaaac
     2641 aaaacagtga tcaacaaccc gtgaatacta aaaggtgtaa aatgcgaaat attgagatct
     2701 aactgatcca ctaataatcg gtggacgata cttgtcaatc ctacaccgac agctgaaccc
     2761 actaaagtag ctgcgatccc tactaagaat gtttcagaaa aaatcatctg tgcgattttt
     2821 ctgccttttg ctcccagcat cataaacatt gcatagtctt tttgccgcat cgtcatcaaa
     2881 aaggaatttg catataaaat ataaacgaat gtgatgatcc ctagtaaaac agaaccgaat
     2941 cggaaaatga taactgtagc cgcaacgatc gtattacttt tcaaaaaagc ttcgttgctt
     3001 gccattgatt caaacatgta aaaaatagca gatgccataa ctaatcctga gaataacacc
     3061 aagtagtctc ttaaacggct cttgatccca gtaagtgata atttccaaag catagtcaat
     3121 ctccccctac tgttccaagt ttccaagaat agatagtatt tctcgataga aggaatcacg
     3181 gctttgatct ccacgtttca cttcttgatg gataccgcca tccttgatga ataaaatacg
     3241 ttggcaataa cttgcagaaa acggatcatg cgtcacaaga agaatcgata catgatcttt
     3301 tgtattcaat tcgtccatcg tatctaaaag atcccgggca cttttggaat ccagcgcacc
     3361 tgtaggctca tcccctaata gaatcgttgg ttgtgtgatc aatgcacgtg cggcggcgac
     3421 ccgttgtttt tgtccaccgg aaatctcaga aggatagctt tccaaaatat gagagatgga
     3481 taaacgttct gcaatttttt ggacacgttg cttgatttct tttggtctga cgccttgtaa
     3541 agaaagagga acagcgatat tttcttgtgc tgtcaagctt tctaataaat tgaaatcttg
     3601 gaaaataaag ccaatctcat tcgcacgaaa atctgctagt tgatttcctt ttaatgtggt
     3661 cacatctttt tgattgattc ggatatgccc atctgttggt ttatctaatg tggataaaat
     3721 attcaaaagt gtcgacttac cagaaccaga agcgcccatg atcccaataa attcgccttt
     3781 ttctacatcg aagctgatgc cgcttaatgc ttgcgtcttt ttctcattgt tttttccgta
     3841 tacttttgtt acattctgta cttcgactat tttgttcatt tctttcccta ccctttaatt
     3901 agtttttcca gtgctgtttt cggcacgtct tttttttcga cttcttcata cgaagtgaca
     3961 cctttatttt tgttccaggt gatttttaaa tatgctcctt tacgaagcgg tcgagtcaat
     4021 tgtcccttga agtccagttc tttttcctta ccctctttcg tatagccagg caggatatat
     4081 ctatagttga ctgtttcttg tccagagtct gattttgaaa ctgtttttgt accatctgtc
     4141 gtaatttgta catagtattc ttctccaccc ataactacgt aatctgcgat tttcacccct
     4201 gcaagtacca caacgatgaa aacaagtaga ccgatcagaa gttttttcac tttttcccct
     4261 tctctcttac tttatatact gattatagaa aaaagcgaac aattctgcct tcgaatcaac
     4321 tatcattctt ctttcaattt tgtcattttc tttaaaacaa ctaaaaaaag agttcaagac
     4381 aataatccgt cctaaactcc ttcttcctat cttacaaaaa gtatgtcact tacggtcttt
     4441 catgtcgcct gtattgataa caggttgcgt tcctttgtct gtaatgattg agacaagtaa
     4501 attattgatc aattggactt tccgatcatc ggtgaaattg atttcttgtc catcttcgat
     4561 ttgctctaag gccatttgag tgattgtgac agctccttca acaatggttt gtcgagcaga
     4621 caaaatggct tttgcttgtt gacgttggag catggcacta gcgatttctg ttgcataggc
     4681 taaatggttc aagcgtgttt cgattacttc gacacctgct actgctaaac gctcttgcag
     4741 ctctttttgc aactcgtcag acactgccgt cgtgtttcct cgaagcgtca aatcattctc
     4801 attgaatgta tcatacgggt attggctagc gatatgccga atggctgtct cactttggat
     4861 ttctacaaaa tcttgataat acgctacatc gaataatgcc tttgctgtat caacgacttt
     4921 aaagactact actgctgaga tttcgatggg gtttccatcc aaatcattga cttttaaaac
     4981 agaactattg aaattccgaa ctttcaacga aacagtcatt ttttgtgcca aaggtatcgt
     5041 aaggaaaaag ccattttctc gaatcgtacc taaatattgt ccaaaaaaca aaattacttt
     5101 tgcttgatta ggactgacta cagttgccga acttaacaac aataacgtga tcaaccaaag
     5161 aaaaatactt aaaaaaagtg cccaaacttt cgcttgccac atacctaaat agaagaaaaa
     5221 caatccaact aaagctaata cgaccaatcc gacaatccct gcataaccat tgatataaaa
     5281 tgctcttttt tctctcatgc tacttcctgc gctcctttat ccttcaataa atatctaatg
     5341 ttactttttt aatttttttt tcgaatacgc gccattacac cttctagttc taactctttg
     5401 tcttcagtga tgtaccaacc gcttgcctgt gtccctccgg ctgcctgcgg taaataatcc
     5461 aattttgttc gaacccagtg cttttcttct gtaaaatagt cttctaccaa gacctctaga
     5521 tccatccctg ctgaaagagg caaatcttcc cgaacagatt taaagataaa tcgttccttc
     5581 ttcccatcat acttcaatat tcctgtatcg ataatcggta aagaataata atccagctct
     5641 ttcttcaccg tcaataaatg tttgaaaact tcatggtaca cttgctctaa ttggcgacct
     5701 tctgaattgt tcgggtaatt aaccatcact ttttctttgt ctggatcata gaaggtttgg
     5761 cttagttgag tgatataagt atgtaatctt tccatttctg tttttaactt ttctctttga
     5821 ccttcttcta ccttcatata ctctttcacc ctgttttctt tttagatatt ttctttctta
     5881 ttttactata gttatgtgcg ttaagcgatg aaaaacagca aagattggaa ggatctttgc
     5941 tgttttttct taaaatatcc agttgagtac aggatcaatc tgtttcattg tcctcttgat
     6001 ccgtcgtaat attgatcgat gtgtctttag cagtgatctt tagctttttc tgtccttgtt
     6061 ctaacaaagt atttgttatt ggctggtagt ctttttcaat ataagattca ccagacgttt
     6121 ggatagtata gccactgttt tccatctcat gcaaagataa attgccaccg tttaggatga
     6181 cttttccttc tttgctgatc aaactgtctt ctagatggaa gttgctgttt tcacctgtga
     6241 cgatcaagtt atccacattg gcctcatcta atcgaagtga gcctaattca tctttcgcag
     6301 ttattgttcc tcctgtaaat tcattgaacg atacccagcc atcatgtgtc ttagtattca
     6361 cttttttgat tgatacttcg ttgatatcaa ttgcactatt attcccttcg attgtcaatt
     6421 cagataattc atgatctgca gggacaacaa tagtgatttg tgcacttgtg tcttctaaac
     6481 taatgattgc acctgttgat tttttgactg cattcacagt caacacgcca tttttctcct
     6541 ttatttctgg cttttctgtc ttatcataag tcgtttttat ctcaaaatcc aatccgcgta
     6601 tgaccgaaac tgtttgattt tctgcatgga tgacgatttt gtttgcatcc ttgatttttt
     6661 ttgtctcagt aaccatatcg atcaaccgag gaccattatc ccaagtcaga ctcttttgag
     6721 cgcctaatgt ataagttgca cctgctagga ggccaccagt aagcatcaga ccaataccgg
     6781 taatcaaaaa atgtttcaat ctcaatttat gtgtcctcct tcaaacgccc gctgaatgca
     6841 gatctctttt tgtaataaaa cgtcgtccca tccatttaac aaagttcatc atgacataaa
     6901 aaagcccttt tgttcctttc agcaacagtg gcgaaataaa tactgcgctt cctgtggcga
     6961 acagaccgac accgataaag aataatccac tcagaatcga ttgtccaaac acactgattc
     7021 ctgagatgat catgaatagg ccaacaccta agatactgat acataaaaca tagatactaa
     7081 aaatgagcgc tagtaaagca accaatagtc ccccaataac caatataaac cccagtgcta
     7141 gtggaatgag gacaggagaa gcaaataaac cgataatgat taaccaaatc aggcgaattt
     7201 gtctttttgg atttttcact aactcgtttt ctgcttgttc ttcttctgaa aagtaactga
     7261 tttttaattt taaggcaaac tgctttggtg taccatattc atcaatgatc tgatctaatg
     7321 tttttccaga atctaaaaaa tattcctcat aatactggat caaatcaaaa cgatcgtctt
     7381 caggaatttc ttgaagatga tccacaactt cattcaaata ttcattcatt tgtgctctcc
     7441 tccaataacc ggtctaaaga atctcgaaaa acaatccatt catcaataat ttcttctaaa
     7501 tgacgacttc ccattggtgt caacgagtaa tatcgacggt ttctgccttc aaaaggttca
     7561 tcataggtct caagatatcc ttcttttttc aatcttctca aaaccggata tagtgtagat
     7621 tcactgacag agagactttc ttgtacgatt tttgttaagg catacccata catatcttcc
     7681 ttgcttaatg ctgctagcac agtaccatca agaagaacag ttggaatctg gatgtgcatt
     7741 acgtcacctt ctattctcaa gtgtaatatt ttatttcaat ctatattata caacatataa
     7801 tatagtttca aaatcagttt gtcaaataca aaaaaactga ctaaaagatt tgatcctttt
     7861 tagtcagcaa aaaatggctt ctctttgatt tttattataa cagtggcata atccaatttg
     7921 tccacatcca gctcagtggc atgataacga tcattgcagg tatcatgtca gctactggga
     7981 acattttcac gttgatcatt ctaaaaccgg tagcgaccat caagaatccg ccgcaagctt
     8041 taaaatctaa gatcatgtct ggtgtagtca atggataaat aaattttgcc aataagaaca
     8101 aaatataaaa aataatgaat tggggaattg ctatcacaga aacgacatac cctaaattac
     8161 acgcaaaaat agctgctgtg aaaaaatcaa ggacagattt agaaatcaaa atcgtactgt
     8221 ctcctgtcat cccagaatct aaacttccat agatccccgt accacttgca caaaatagaa
     8281 caatgaccgt cactaatgtg ctgataaatt catcgtgggt cataccaagc ttttcactag
     8341 ggaagatttt agaaataggt acttgcatca gcgcagcccc cttatttatc ccattaccta
     8401 aatgaacaat caaaccaatc gctgttccta tcactaaagc aaaaataaca gccggcatat
     8461 ttttcatcgg cgcaatggaa tagattccca tccccatcga acacacacca aaaaccatcg
     8521 tcagctgtgc tttaaaatgt tcacttaatt tatttcctac taatccgcca attagtccgc
     8581 caaacaaaac ggaacctaca ttgatcaaaa ctccagttgg catcaaaaaa ctcctctctt
     8641 tttctacgaa gcgaaaaaaa tcagaaaaat ttctgtaatt attgctttcg ctgcaagaaa
     8701 gattgtaaca gacagagaag attacaccaa ttcatttttt gtgttaaagt attctttatt
     8761 ggaataagta ggaggaatat gatggatatc cataaattcg aagtttttct tgatttagcc
     8821 gaaacaatga atttcaccaa aactgctgat cgacaattca ccacccaagg aaatatttct
     8881 aaacagattc tttcgttaga aacagaatta gacgtcaaat tattcgaacg cgcccataga
     8941 aaaatcgaat tgaccgaagc tggaagctta ttgctcccat atgtaaaaaa tgtcgtagaa
     9001 caataccatg ctatgcaaga agtgcttgaa acctatacga aagacaacaa tctttcatta
     9061 aatatcttaa cgataccgac aatgatcaat tataaaggtt tttccaaaat caccgagttt
     9121 ttgaaacgcc atcctgaatt tacagtccag ttgaaagaag tggaaagtgt cgaattaact
     9181 tacaatgacg ccaatgaaca tagtgacacg atccactttg ctagaagttt ccaggcccca
     9241 ccaagttcga ttgagtggtt gcctactgaa acagacgatt ttgtagcagt tctacctaaa
     9301 aatcatcctt tagctagtaa aaagaaattg gatctaagcg aactgaaaaa ggagaacttt
     9361 ttgctccttg gccccactac gaacttgtat caacctgtac tagatctgtg tcgcgaagct
     9421 ggatttgaac caaaaatctc ttataaagga gcaagaatcg acttgatcat caatatgatt
     9481 gcaaatgaca tggggattgc tattgttatg gaaaaaacag tcaagaatct attaaaagaa
     9541 aatactgtga ttctccctat ttctccaaca acagagagtt atcttgcatt tactcgtaaa
     9601 gtcggtgagc attctttggc ttcagataca ttttgggagt atttaaatca agaagaaaac
     9661 taagccctac attttctgtc catctatcca cccaaaaaaa gagagcgacg catgactttc
     9721 gctctctttc ttgcatcatg tccttgtttc aatgacacct ctaacatttc tcgtagttca
     9781 ataagaaagc agcgattttt atgccaaaag aaccgtaaga acatttccct aatcacaaaa
     9841 aaacgactaa aaacccctaa aaaccttcaa tagaattttt aggggtttta ttctttttcc
     9901 tattgtttac ttaaccgtat cattgctgtc gttcctactc cttcgatact gctataatca
     9961 atacgtccat catgcagttc tagaattttt ttaaccagtg aaagtcctag cccattccca
    10021 gaaatttgac gacttgtatc tccttgataa aatttttcaa acattcgatc tctggtctct
    10081 tcattcattc ctacacctga atcggtcact tccagtacaa tctctgtggc tgtctcaaat
    10141 agtttgatat gaatctgtcc attgatttga ttgtacttta tggcgttatc catgatattc
    10201 agccacactt ggtacaaaaa ctcttcattg cctgtgtaat ttactgcagc aagttcgata
    10261 tccaattcta gctgttcttt ctcccattta ggctgaaaaa aaacgattac ttctctgatt
    10321 tgttcatcta atcgatactc tttcttttcc agttggatac gctggttttc cagtttgttc
    10381 aattttaaga tgttctctgt taattgtgaa agctgcgtga tactttcgat aatccgatat
    10441 aaaaatattt ggcgttcttc gtcagagaga tttggggctt gtaacaattg aacatatcct
    10501 tggattgttg ctagtggtgt tttgaattca tgggatacac tggacacaaa gtcattcctc
    10561 agtgtctcga tactattgag ttcttggacc ataacgttga aatctttata cagttgctgt
    10621 acttctgcta ctttctgctg ttcatccatt cgaatcgaaa aatctcccgt tgctaccaga
    10681 ctcatatttg tccctagcgc actgattgga tgcaggatac gccgcccaac aaaaatcgat
    10741 attcctgtac ccacgaccat actgaacccc gcaaagacga ataacgggta taacgttggt
    10801 tttccagccg attcagcagg cgagatagaa tgtcgagaca ataagaaaat aaaaccgaga
    10861 aaacacagca gtgtcactaa gatcgaaatg aagacaatac tagcaaaata gatccataat
    10921 tgtgaaaata gctttttttt catctttaaa taaccgcctt atagcctagt ccccgtactg
    10981 tgatgatttg aaaatcttcc gtcttttcaa aacgggtcct taaacgtttg atatgtacat
    11041 cgatcgttcg ttcttctgta ttcgtatcta atccccaaat atcatccatc aactgttggc
    11101 gagtgaagat cttgttggga taagacagta atttatacag aagcagaaat tctttttgag
    11161 gcagaatcaa cgaatgctga tcttgtaaca ctgtataagc atctagctct aattttgttt
    11221 gtccgattat caagctactg ctgtaattga tctttgaacg tcgaagcaat gcttccacac
    11281 gtaggatcat ctcattgaca tcgatgggtt ttgtcatata atcatctgct cctaatttga
    11341 acccgcgttt cttatcctca aaatcggcct ttgccgtaat cattagaaca ggtgtgtcaa
    11401 atctggtgct acgtaatgag tctagcaagt catagccatc cattttcggc atcatgatat
    11461 ctgtaatgat taattctata cgtgtcgctt ctagtttttt cagcgcatcc tctccattca
    11521 atgcggtgat gactgaaaag cccactcgtt ccaacacaga acggtaaagc aactgtaatt
    11581 gcggatcatc ttctactact aaaatacgat tcattgatct ccctctgttt tttgaaaaat
    11641 ttctagattt tttcttgtag ttcatcttca gttaacattt atttcgtata ctacgatcat
    11701 tgttctgata tacttattag tataccgaat atttcaatac gggagaaggg tgattatgct
    11761 tcaattaaaa gatataaaaa aatattataa agtcggcgaa accaccacca aagcattgga
    11821 tggtgtgtct gtcgctttca gacaaaaaga attcgtagcg attttaggtc ctagtggttc
    11881 tggtaaaacg actatgctga atgtgattgg tggactagat aattatgatt ccggtgatat
    11941 ggtaatcaac ggaaagtcta ctaaagactt taaggacagt gactgggatg cctaccgaaa
    12001 caactctatc ggtttcattt ttcaaagtta caacttgatt ggtcatctag gcattattga
    12061 aaacgttgaa cttgggatga cattaagtgg tgtttctaaa gacgaaaaac gaaaacgggc
    12121 agaagatgca ttgcaccgag tcggcttgac aaatcatatg cataaaaagc caaatcagtt
    12181 atctggtgga caaatgcagc gagtggccat cgctcgagca ctagccaatg acccagatat
    12241 ccttttgtgc gatgaaccaa ccggagcgtt agatacagaa acaagtatcc agatcatgga
    12301 attgatccaa gagttatcga aagaaaaatt agtgatcatg gtcacacata atcctgaact
    12361 ggcccatcaa tacgcagatc gaatcattga attttctgat ggtaagatct taacagattc
    12421 ccatcctcac atcgaacgac caaaagatga tcaatttaac ttacgcagaa caaagatgag
    12481 tttttggaca gcgttaaagc tttccttcaa caatatccga acaaaaaaag gacgtacttt
    12541 cttgacctcc tttgcttcca gtattgggat catcggaatc gcgatcgttc tgtccctttc
    12601 ttctggattc caaaaacaaa tcgataacac gcaagctgaa acgatggcga aatttccgat
    12661 tactatttct aaggtaacga ctaaccagac tcgtgacgat gccggattgg gtgcaagcaa
    12721 agcggattat ccagacagca aaacgattac tgcaaaagtc agcgaagaag ataaagcaca
    12781 gcacacgaat aagattgatc aaacatacgt cgattacgta acagacatcg atcctaactt
    12841 aagtaataat atcggattta cccgtactac aggaatcaat cttttacgtg atgtaaatgg
    12901 caaagtacag cctgtcagct tctcgaatca aaatcctgat acggaatctc tttctttatc
    12961 tagtacgatg tcagcaatga caggtgtggg agtgtcaagt ttccctacac agcttgacac
    13021 tagcaaagaa aactttttaa aagacaatta ttctttactt gccggaagtt atccagcctc
    13081 agcaacagat gtcgtgctga tcgtagacgg caataataat actaatatca acgcattgaa
    13141 gaatcttgga tttgatgtga aagaagacga aaaacttgat tttgatgata tcgttggtac
    13201 aacattcaaa ttagtaaaca acaatactta ctatacaaaa cttccaactg ggaattttat
    13261 ccctaataca gattatgatg cgatgtatca gaatgcttct gatgaattga agatctcggg
    13321 gatcctacgc gtgaaatctt cttctacaat gaatctctta tctccaggaa tcgcttatag
    13381 cgatcaatta acgactcaaa tcgtcaacga aaacaaagaa tcagaaatcg tcaaagctca
    13441 aagagacagt ggtgtaaatg tcttaactac tgaaaaagtt gacgaaaatg ccaaacaaac
    13501 attgcttagt tatttaggtg gagacagctt gccttcaagt atcatgattt atccgaacaa
    13561 ttttgaagac aaagaaaaga tcttggatta tctagatgat tacaataaag gcaaatcaga
    13621 tgaagataaa atcatttata ctgatttagc tggaacgatg actgaactga ctggcggatt
    13681 gatggatgct atcacttatg tccttattgc ttttgcaggg atctctttag taacaagtat
    13741 gatcatgatc agtatcatta cttatacatc tgtgattgaa cggacaaaag aaatcggtgt
    13801 actaaaagct ttaggtgcac gtaaaaaaga catcactcgc gtatttgacg cagaaacgtg
    13861 tattttaggt atttcttctg ggatattagg tgtgtttatt gcatggctag ctacgttccc
    13921 tatcaatagc atcttataca acatgacgga tctaaaaaat gttgctcaac taaatcctgt
    13981 ccatgcgatt attctagtga tcgtctctac tatattgacg atgcttggcg gtcatcttcc
    14041 tgcacgaatg gcagcgaaaa aagacgccgc tatcgctttg cgtgcagaat aaggttgtga
    14101 caaccacatt tagatttgag tcataagagc gctgaaatag tagaaaacag caagaaacga
    14161 ggaagtaata tgttccttgt ttcgcgctgt tttttttaat tattttttat aattctggta
    14221 tacttttctt tcctttgtac cacaactctt ctggcgtata cctgtctttt ccatttatga
    14281 atactgttcc atctgcttca atcgttactt cagtcccaat ccacatatta cgagaaagtg
    14341 cgagaagatt ttttcttttt cctcttcctt tttttccaaa ttgcaaggca tcggttatct
    14401 cttctgtcca atgagatttc acgtatttta cttctccgtt ttcaaatgta actttcaaaa
    14461 tattcgctat attttccact tttactgctt taggaaatga ctcctccatt tttttcactt
    14521 tccttcctcc aacactaagt atgcgctttc ttttagtgat aagtatatca tagtttattc
    14581 tattatcaaa aaaacgcatc cattccattg tagaatactt taccgaacaa gttgaattgt
    14641 tttcctcttt ttcacctttt actatagata ggtaaagaca actaaggagg aatctctcat
    14701 gacagatcaa ccgtatgatt taagaaaagt catcgaagaa ctaaaaacac ttccaggtca
    14761 atatcacgaa acagatattg aaatcgatcc tgaagcagaa ttatctggtg tttaccgtta
    14821 tatcggtgct ggcggtacag taaaacgtcc gactcaagaa ggaccagcta tgactttcaa
    14881 caatatcaaa ggtttccccg gcacacgtgt gaacatcgga acaatggcta gtcgtaaacg
    14941 tgtaggtgaa attttgcacc atgattatcg aacacttggt cgtttcttga aagacgctgt
    15001 agaaaatcca atcaaacctg tgaaagtaga caaagcagac gctccagcac aagaagtcgt
    15061 tcatctagct accgatgcag acttcgatat tcgcaacatt ttgcctgcac ctacaaatac
    15121 tcctgaagat gctggtccat acatcacaat gggtgtagtc ttaggatcag atccagaaaa
    15181 aacaatgagc gatgtaacga tccatcgtat ggttctagaa gataaagata cgatcggtat
    15241 gtacatcatg cctggcggac gccacattgg tcacttccaa aaacaattcg aagccttgaa
    15301 taaaccaatg cctatcacta tcaatattgg tttagatcca gcaattgcca ttggcacgac
    15361 tttcgaacca cctaccactc cacttggcta caatgaacta tggtgtgcag gagctttgcg
    15421 taacgaacct attcaaatcg ttgatggtat agcagtcgat gaagtgggta ttgctcgtgc
    15481 tgaattcatc attgaagcag aaatcatgcc aaatgaaaca atgcaagaag atatcaacac
    15541 gaactcagga aaagcaatgc cagaatttcc tggctatact ggggatgcta atccagctgt
    15601 acaagtagtg aaagtcaaag cagttactca ccgaaaagac aatccaatca tgcaaacaac
    15661 gattggtccc agtgaagaac acgtatctat ggccggtatt cctacagaag caagtattct
    15721 tgccttggtc gataaagcta ttccaggaaa agtagtaaat gtctacaatc ctcctgcagg
    15781 tggtggtaaa ttgatgacca tcatgcaaat ccgtaaagaa aacgaagcgg atgaaggtat
    15841 tcagcgtcaa gcagctattc ttgctttatc agccttcaaa gaattgaaga cagtcatttt
    15901 agtcgatgaa gatgttgata ttttcgacat gaacgatgta gtttggactt tcaacactcg
    15961 tttccaagga gataaagata tcatcgtgat cccaggaatg aggaaccatc cattggaccc
    16021 atcagaatta ccagaatata atcctggctc tattcgtttc cgtgggataa gtgcaaaaac
    16081 tatctttgac ggtacagttc ctttcgatat gaaagaacat tttgtacgtg caaaattcca
    16141 agaagtagaa gattgggaaa aatatttgaa ataatgaatg atctgcaact gttttgacat
    16201 cataaaaagg agtgcggaaa atggaacaat tggcacaacg gaagaaaaaa atcgtgattg
    16261 gtgtaagtgg cgcttcaggc accatttatg ccatcaacct agtgaagaaa ctaaaagaat
    16321 atccgatgat agaaacacac ggaatcatca gtccatgggc aaaacagaac ttaaagctgg
    16381 aatccgactt atcactaact gaattcgaaa gcctgtttga ttaccattat tcaaacaaag
    16441 atttaggtgc tgctgtcgcc agcggatcat ttttgaccga tggaatgatc atcgtaccgg
    16501 caagtatgaa aacagtcgca ggtatctctg ttggtttagg cgacaacctg attgcacgtg
    16561 ctgctgatgt aacaatgaag gagcaacgaa agttagtcat tgttcctcgt gaaacgccat
    16621 taaatgccat tcacttggaa aacttgacta aattagcacg actaggtgtg caaatcatac
    16681 caccaattcc tgctttttat aatcagcctc aaacgatcca agatcttgtt aatcaccata
    16741 caatgaaact tttggatcaa tttgaaattg taaatgagca ttcaaaacgt tggcagggtg
    16801 attaatatgg aaaaagaaac gatagttgaa gtaaccaaac taggtttcac gatccaagct
    16861 gctgttaaag taatcggtaa agacttattg atcactttaa caggcggaga tacaccacat
    16921 atcggaacag tcactacatt ttctcgtgat acagagatgc aaactgtacg tttccctagc
    16981 cacgatggtc ggttccataa agacgatgtc ttagccgaaa aaatagctgt gatcatcaaa
    17041 actgccgttc cgggaagttg tacgatcact tccggtatcc atgttgacca tatctccaat
    17101 gcacaaatca acgcctcttt tccgatgtca gaagagttag gttacaagat tttagattgg
    17161 ttggaaaaaa tagatttcca tatcaaagaa ccagtttatt ataaagatgg tgaaaaacca
    17221 ttatgaaaga agaggttgtg aaatcagcgt tatgacctga gtatgagaga aaaaaatgga
    17281 aaaatagctt ctcatatttt gccgtttttg agctaatacc gagggacagc tgattgaacg
    17341 ccgtttatca agaggttgtg ataaagccgc ttcgtttcga gtagtaagaa aaattttgta
    17401 aacatgggtt tccacatttt tgcataaatt tgacttacta ccgagaaact ggcttttgaa
    17461 caccgtttat caagaggttg tgacacgtct tttgtcacaa cctcttttat ttttgcttca
    17521 ctgtaactgc tacaatatca ccgatttctt ttccgatgtt tttacgaata tcttttcgta
    17581 gtccaaggat gtagcagact gaaccatctg gatttttcac gcccatattc acgatactgc
    17641 cttcatacgg cacattgtca aaagttgctt ggactttaac tcttccagca ccgaattctt
    17701 ttcttatatc atacggaaaa ataatatatg ccccgccctt gccgacttcg gaagcataga
    17761 tttttgattc aaaagaataa atttccctca ttttatttct cctatttttt acatctagta
    17821 tagcatagca aaaaaaagaa ggaactgtga caaaagtctt gtcgcagttc cttttttcga
    17881 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcaa caaacatgag
    17941 aagctgtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct
    18001 tccttaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag atgaaaaata
    18061 tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct ttttcacaac
    18121 ctcctacatg gtgttcaaaa tattattcgc cttttgcttc tttcactaat tttgcgaatg
    18181 cttgttgttg gaattcagca gctaaggctt tcgtacgttg ttcttgctct gctttgctca
    18241 tttttggatt ttcagtcatt gcttttacca taccgtcttg aacataagct tttgcttcac
    18301 tttccatcgt actttgctgt ttcttcacta attcagctaa cttggctgct tggtccgtac
    18361 ttaattctac ccatgtctct gggattttga atgtattcac tcggctgtca tattcgtgat
    18421 tgttatcagc aatattgaac cagaatttat acatgtcgtt tttgtattcc caataagtag
    18481 ttttcgtcac tttttcagca tcgccgtctg tcttttctac tttatttgtt tcgttatccg
    18541 taccagttgc ttttggtttt tcttgttttt catttgttcg gtaaagatac actttttctt
    18601 ttccattccc taaagcttta tacaaaagca tatctgctcc ttgggaatca gcactggaaa
    18661 caattttttc tgtttttgtt tccgttactt tttccattcc aaagtgattg gacaaattag
    18721 cgacgatcaa acttagtgaa gcgacgaaga ttagtccgaa aatcaaagat aaaaaggttt
    18781 gccatgtttt tttagcgaac acgttcgtta aagcaaaagc aagcaagctg acaatcaaaa
    18841 ctccaataat catgcattat cccccgcttt cattagaaca ggttcttttt tcttcaataa
    18901 aaaggcaata ataaagccaa gaatcccaaa gatgatcgct acgataaacg cggcatggta
    18961 acctgacagt gtggcattga ttgcctgatc tttataagct aatggcgtat tatccaatac
    19021 agatttgctt gggatatttg cttttgtcac attcgtcaac acactgatca aaatagctgt
    19081 accaatcgag cttgcgattt gacggaatgt attatttacc gctgtcccat gactgatcaa
    19141 gttatttggc aatgcattca taccagaagt cgttaccggc atcatgacca tggagatccc
    19201 aaacatacgg atcgcataaa ggatcacgat atataaaact ggagtcgttt ttgtcaagaa
    19261 agcaaatggt gcagttgccg ctgtcaaaat gaacatccct gtgatggcta aacgtttcgc
    19321 gccgtattta tcgaaaatcg cacccgtcac aggcatcata gcccccatga tcaatgcacc
    19381 cggcaacaac atcaagccag aatggaatgc tgattctcca cgaatatttt gaatatacaa
    19441 tggtacgacc atttctgccc ctaccatcgc catatttgtt acaccagcta aaacagctgc
    19501 aatcgtgaat gtagatgatt tgaatacacg aagttccaag aatggatgtt ccaaatgtaa
    19561 ttgacgccat gagaacaagg cgatgaaaat cacaccaaca actaagaaac caattacttt
    19621 caagcttccc cagccgtcat ctcctacact agaaaatccg taaagtaaac taccgaatcc
    19681 aatcgttgat aaaatagcgg ataaaatatc aatgctagga tttgacaatg gaataacact
    19741 tttcattagg aagaatgcta aaaccaagac aagaacaacg attggcagta ccataccaaa
    19801 caagtcacgc caatggtagt tatcaatgat ataaccagat aatgtagggc ctaaagcagg
    19861 agcaagtcca ataacgatcc ctgacatccc cattgctgat cctctttttt ctggcgggaa
    19921 aatagatagc atgatatttt gcaataatgg catcgatact cccacaccgc aagcttggat
    19981 caaacgaccg attagtaatg tactaaaatt aggtgctgta aagcaagtaa tcgttccgat
    20041 caaaaagatc gtcatggcag aaagatataa atttcttgaa ctgaatttat tgatcagcca
    20101 tgctgttatt gggatcatga tcccgttgac tagcaagaaa ccagtcgtta accattgcac
    20161 atcagacgct gaaatatcga aagctttcat caatgtagga aaagctgtcg tcaataaggt
    20221 ctgattcaaa acggtacaaa atgtaccaat taataagaca acaactaaaa gtgtccgatt
    20281 atatggtttt ccgtaaatat ctacgggttg gttgttactc atatttctcc ttctttctga
    20341 attttttctg aaactactag aatactttag tcctaaattc gctatttgtc aacctacttg
    20401 acacataagt taatacggat ataattaagg gaagcaagag gacaaaagga gtgacaaaaa
    20461 tggttggggc ttcccttaaa cgtttgttgg aaaatgatca attggttgtt ggcatcagtg
    20521 aattaagtga tatcgtaggt gtgtctccac gtcaattaag gtattgggaa caaaagggat
    20581 tcattcgttc gatcgaagct gatgcgaatt gtcctagaaa atatcgttta cctacagtca
    20641 taaaagtaga actgatcaaa aaatatttag atgacggatt tactttgtca aaagctgcag
    20701 aaaaggcaga aatccgccaa aagaaaatgc accatgtccg taaagttttt tcaaaagcaa
    20761 tcaaagatat cgaattgatc gacgaacgct atacgatttt acaaatcggt aacttcaaag
    20821 aagagaatgc taagatgtac atcattcatg acaacgagac agacgaatta acttacaagc
    20881 tccttcctgt cgatgaaaaa gctgactacg aacagttgtt aaaagaactc taaacgccac
    20941 taatctccta agtaccaagc tttttaataa gtagatgcta cataaatact tgaaaaaagg
    21001 cctgtttgaa cataagaata tccgaacggt gatctgcgtt tctttcatat agaaaggaat
    21061 tctccatcac tgttcggata tttttttatt tttttcggtt actttcttct aatacatctg
    21121 ttagccaatt tgcatattct gtttcccgat tgatcgcatg cgctaaaatc aggtagtgtc
    21181 cgtaattttc atgtttttct ttttcagtag gaaagatcgt tttttttcgc tctaatagat
    21241 gaagtcgttt ttcttcatga aggtcacgct gctgctgaat aatttctact aatgccggat
    21301 cattcacgtc tttgacgaaa tacaatttca gaacaaattc gtctcgatta accggtagtt
    21361 cattggtggg tgtatgaatc cactctgcca attgttcctt cccttcttct gttaattcat
    21421 aaactttctt ttccaatttt tcacctgtga tctctacttg atgctgaatg attccttgct
    21481 cttccatttt tgcaagttca ggataaatct gactatgttt tgcttgccaa aattctccta
    21541 tttcattttt gaacgattga ttcaattcat atcctgtcat cttcttctta cttaataacc
    21601 ctaacaaaat atatcgaagt gtattttttc ttgccatcag ttttccctcc ttgacaaatc
    21661 tttaaatcca ttctatcata aatgagtaat cgaaaacatg tagtttatta catgttttaa
    21721 tttataagga gatgcttaaa catgaaagaa tttaaagaat tggaagattt tattggaact
    21781 cactttatct atacgtatga caatggttgg gaatatgaat ggtatgccaa aaatgaccat
    21841 actgttgatt accgtatcca tggcggaatg gtcaaaggcc gttgggtaaa agaccaagaa
    21901 gctgatatcg ttaaattgac ggacggcgta ttcaaaatca cttggacaga gccaacaggg
    21961 acagacgtgg cattggactt tatgccaaat gaaaagaaac ttcacggaat gatctttttc
    22021 cctaaatggg ttgaagaaca tccagaaatc actgttactt tccaaaacga acatatcgca
    22081 gaaatggaag cagctcgtga aaaatatgaa acttatccaa aattacttgt tcctgaattt
    22141 gctacgatca cttatatggg tgatgcaggt atagacaatg aagatgttat tagcgaagaa
    22201 ccatacgatg gaatgccaga tgatatccgt agcggaaaat acttcgatga aaattataaa
    22261 cgagtaaaat aaggttgtga aagagaggtt gtgaaatcag cgttctgacc tgagtattag
    22321 agaaagaaac ggaaaaatag ttcctcatgt tttgccgttt tttgagctaa taccgagggt
    22381 cagctgattg aacaccgttt atcaagaggt tgtgaaatca gcgttatgct ttgagtaata
    22441 agaacgctag ttagaaaata gcttttcata ttttctaggc tagtgggctt attaccgaga
    22501 agcagccgtt tgaacaccgt ttatcaagag gttgtgaaat cagcgttttg acctgagtat
    22561 tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc taataccgag
    22621 ggtcagctga ttgaacaccg tttatcaaag attgtaacaa ctgcatattc acacagttaa
    22681 attagtgact tcaaaatgaa ataatgctcc cagcaaaaaa ttaggctggg agcattattt
    22741 cgttattctg ttcatcaaaa atcagcgttt tgattccatt tcatcacaag ttcctcctca
    22801 tttactgctt catctttttg ctgtgtttga attgtaaaac cttgtgaaag atagaaatga
    22861 atagctgatt gatttttttg atagactgtc aaagtcagtg ttttattggt ttcttttatt
    22921 tttttcatta gttgcgtacc gattctttgt tgccggtatt cccttttcac aaagattcct
    22981 gcgataaact gatcaatcac cccagcaaaa ccaataattt cttgatctat ttctgctaag
    23041 taaatcactg catctggtaa agctttccta acagattcta cttgattctc ccaataagtt
    23101 gatggaatga attcatgaga atccaagtta gcatgcagcc aaatcgatag aacagattcc
    23161 agttctttgg cagacaattt tgttgttgtg cggatcattt tttctctcct tacttattca
    23221 ttgattttaa aataaaaacc atttttttga agctctttta caagttttcg cccctcaaaa
    23281 aagtatatct tctcttgttt tacttttcta ataccaaaag aaacagcaac tttaggatca
    23341 tcagctgaaa cgattcgata agttccacca ggcaattgat ccagcttgac tttcccatga
    23401 tggtcgcttt gtactgaaaa cggcacacca tttctagtaa gtgttttttt cttatgtttc
    23461 aaaatgaatg aactaccatt taccggtttc cctgacgaat cactaataat aaatgaaaaa
    23521 tcaagcactt ttttacttaa tctatactga tagattagtc gtccgataaa cactaacagt
    23581 atgactacag ctattatcgt tcctgcaaga ataaaccatt gccgcatggt tctttcctga
    23641 tttccttttc cttcttcttt ttttaccgat tcgttataag gaatccgatg tcctgtcact
    23701 agcaaacgat gcgtgttgat catatacggc gtacatgtaa gcaaggtaac taagtcttct
    23761 ccttctgaaa tcgttagaac agacgtgtca tctggcagta ccacttgtat atttttaact
    23821 tgataagcca atttctcccc caaaacagtc aaaataaacg tgtctccgtt ttctaaacga
    23881 tctaattcgg taaataaccg tcgattaggt aaaccgctgt gagcagagat cactgaatgc
    23941 gtcgaatttc caccaagcgg gaaagaggtt ccttgcaaga cgcctgctcc attttcaagg
    24001 atcctaccat tgagccgatc ataaagtgaa atatttaatt ggatcttagg tatattgata
    24061 cttccgatca gccattcctc taattggctg gatgttgtac tttcatttct gttcttttca
    24121 ttgaaaggat cagtaccagg attgaatcct tggtcagtta atcgttggtt tttctcctcc
    24181 attttctttc gctgcacttc aatttcagct gcggtccgtt tttgtacttg ttcgatacgt
    24241 ttttgatcaa taaaactatt taaagaatcc acataaaatg gatataaagc gatcagtaac
    24301 cctacgacga tacatattaa taaaaacaaa cgaaacaaaa gtcgacgaga ctgtttttgc
    24361 ttactctgct tcacatgtgt cctcccttgc aagttttttt gttctttctt ttattatatg
    24421 ggttcaggaa aaaaatagaa aatgaaagcc gatgaggttg tgaaaaagct gtcctgcatc
    24481 aagtaataag tacagcccaa aaaaatagct actcatattt tttgttgggt tgggcttatt
    24541 accgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgaaaac tgcgttatgc
    24601 tttgagtaat aagagcgcta gtcagaaaat agcttctcat attttctagg ctagtgagct
    24661 tattaccgag aagcagctga ttgaacaccg tttaccgaga ggtcatgaaa aagctacttt
    24721 gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag atgcaacaaa acttatgtcg
    24781 catcctctct ccttagaact ttcacttatg tttttcttgt aaaattcata aatattttca
    24841 tgaattttct aatttcttgc ctacctctcg cataggatat atgatgaatt tatctaaagt
    24901 ttgcaaagga gttcgacaat tgaaaaaata tggcccattt ttagcaattt ttttcattgc
    24961 tttggcattt ctcatgcctc atttattcac acataaacta attttgggtg ctgatgcccc
    25021 attccattac aatcggttct atgatgccgc gcaacaaatt aaatatgggg atttccatta
    25081 ttttccttcc ctttatggat tccagcaaag tggcaggatc gtgaatgccg tttacggccc
    25141 atttttcgct tatttacaag gattgatcgt gttgcttgca ggtagctggt ttcattatca
    25201 gattctttca aatttcattc tttatcttat ttcaggattt tctatgctga ttttactgag
    25261 atatgtcaat gttcgaaatt gggtaagtgt accagtagcc gttttattca tgagtacgta
    25321 ttctattcaa tactggtcta tcaaccaagg attcactagc tgggggactt gtttttatcc
    25381 tttatgtatg atccctattg ctgatttcgt ccttcataaa aagttccctg ttatcaaagt
    25441 cgctgtctca gtgggactta tgacacagat ccatttactg tcttcgatca tgttagcttt
    25501 gatgtatatc cctttttatc tttattactt ttttaaccag actgaaaaag gaaaagcact
    25561 gcttcaactg attaagtcgg tcttgcttta ttttgttctg acagcaaata tttgggcagg
    25621 attagctatt gtttacagtg ggaataagat tcttcctccc tttgttaaca gagaaatgag
    25681 tcaaaaggcg atagatctgg atggcagtta ttggctccat taccctaagg cctttcctat
    25741 attgatatta tcaggttttt tgctttttat tttctttcaa caaaagaaca ccttattcca
    25801 aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt tctactagct tgattccatg
    25861 gacgacattg atcgaaaaaa atgtgccttt tattagtttg atccagtttc catttcgatt
    25921 tttcgtgcca tttacgctat tgcttctact ttaccttgcc ctttcactga accagtggtc
    25981 tgaacaaaag tggttccgtc ttttaagtat atttggcatt cttatttttt cagcacagac
    26041 gatccagaac ttataccagc atctggaaaa gtgggaaaat gaaacatttg tcagtcgaca
    26101 tacttatcta ttcgatacgc cggaagaagc aaggaaaaca ttcttcagca aagatatgtc
    26161 taccagcttg ttcgttttcc aaaaaaccac acctgactat ctgccaatct ataaggagac
    26221 aaaagacaat aaatacgacc ggtataacga agaaatacta gaaaacgaaa caacttttat
    26281 aaaaacacat ccaggcggtc ttttgacaat caagtggaaa aacactgggg acaaaactgt
    26341 tcatatccct gttatcgtct acgatcggac tgtgctacag caaaatggaa aaacattgac
    26401 tgattatgaa gtaacagata tcgggacccc aatcgtgaag cagcaaaaag gaatcaacga
    26461 gctcacgctt cattatcaaa cgccaatcta tttctatttc attctttctc ttactttgat
    26521 tggctggttt accttgctat gtttatttat ttatcataga tacaagcttt tacgtgctta
    26581 ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa acggtataat cgtttggaga
    26641 gttttatttt aatcagtttt tttatgttat atgttatatt ttttattcgt tcacatctaa
    26701 ttcgatttcc accccgtagt gatcactgat gattggacta tcgatcccat caaaaatccg
    26761 ttgatatttt ttaacctgca ttccttcagg gacgaaaata aaatctatac gcaatttttc
    26821 tgtattttgt tcccaaccat ctattttctt ttccacagta gctgcttttc ctttttcatt
    26881 cgcgacaaca tacgaatctt gaagaagaag gcaactttct cttacagtat aataaccttg
    26941 agagtctact ggattattga aatcacctag gaaaaaaagt ggtacgcgag tttccaaaaa
    27001 gtagttttcc aagtttttcc actcaaaata aaagccactt tttttatctg tccaccaaga
    27061 aaagtggcag ctcgagacaa aaatgtcctg atcatctatc tttgttttcc caatcaagat
    27121 cttgcgtgtc tgataatctt ctttcttttg aaattgggag acggtaagga cctgactttc
    27181 caatgttttt ttcgtaagca gtgcatttcc ttcttcatat ttatcataac cgatgtggct
    27241 catttcccaa ctccaaaaat actcttttcc gtgttccttt aaaagctgaa ctaaacggta
    27301 ggcaaaatta tctttacgga taggtacttg attctttacc ggacagaact tcattagctt
    27361 cgcaggttct acttcttcag attccagtaa ttggttgatt tcttgtaaag caatcacttc
    27421 ataatcttct aacaggatct tatctgctaa ttcttgcaat tttttctctg gttctggctc
    27481 aagccagcta tgcgtgttga gtgtcatgaa tttcattggt tttctccttg tcttcatttc
    27541 gaaacaaaag ggttgtgaca aaatttttgt cacaacctct ccagcaaata gtgttctcct
    27601 gctaactcct cagtaataag ctcaatggac caggagctat tttttgtttg gctgttttta
    27661 tcacttggtg cagggcagct ttttcacgac ctctccacta aacagtattc aaactgctgc
    27721 ttctcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
    27781 ttatgacttg atgcaggaca gctttttcac aacctctcct tacattttaa tggagccgat
    27841 cactttacca gctgtcactg tacctaattg cttgatcact acttcttcta ctttatcgtt
    27901 tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt tggatcacat caagatccat
    27961 ctcagctaat ttcgttcctg ttcctacttc ttggccttct ttgacagcaa tctcaaaagc
    28021 tggctgattc atttggactg tatcgattcc catatggatc aaaacttcga taccatcttt
    28081 cgtttccagc ccaattgcat gtttgctagg gaaaatactt gtcacttttc cagcgatcgg
    28141 cgcaacaact tctcttgatg ttggaataac agcaaagccg tcacccatca ttttttgtga
    28201 gaataccgga tcttttactt cagataaagg aatcacttct ccagaagctg cggaaactag
    28261 ttcttgttct atacctaagt attgcttact acctgcttgt ccatttcctg tagaaaccat
    28321 ttctggctct ggaatctctg ctcctgattg cagcagatct tcgatatctg atttcaagac
    28381 atctgctttt ggtccataaa ctgcttggac tccattgtct ttgacgatca atcccatcgc
    28441 ccctgctcgt ttccaggctt cttcagtccc tactttttct cgatcattta cacttacgcg
    28501 taaccgagtc atacaagcat cgacatcttt gatattttct tttcctccca gcaaatgaat
    28561 gatctggacc acttgctgat ctgctgttcc tgttccgctg ttagatacaa cggtttcttc
    28621 actgtcattg tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa
    28681 gaaatttgct aagaaatacg taacaacacc aaaagcgatc acacacaaca cgaagttgat
    28741 aagatctcca cctagacccg ctttgatagc taacggtgtc cgagtcagca attcgatatt
    28801 cccaaatgaa tggacccgca atggcaagat atctgccatc gcaaatgcag ccccttggat
    28861 cactgaataa atgacatata atggcacagc agcaaacata aacatgaact ccaatggttc
    28921 tgttacacct gttagaaaga cagccaacgc tgctgaaaaa tacattgatt tatatcttgc
    28981 tttcttatca ggatcgacat tacgatacat cgcaaaagcc attcccatca agatacctga
    29041 agaaccaatc atttgtccta ctttgaaacg agccggcgtc cagttatgca acacaaactc
    29101 atattgcgac atatcccctg cacctttaag attcactaaa tcagtggccc aagccaacca
    29161 taacggatct tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga
    29221 tccgccaagc tgtgtatagt tgataggaat cgtcagcata tgatgcaatc caaatggcaa
    29281 tagtaaacgt tctaatgttc caaataaaaa cggtgctaga atcggtgcgc tttcttgtga
    29341 ttctgcaatc cataaaccaa aattattgat tccagcctgt acgtttggcc acaccacagc
    29401 aagaatcaat gccacgattg ttgaccataa gataacaaca aacggaacaa agcgctttcc
    29461 attgaaaaat gacaaagcat ccggcaattt tctataatta tagtacttat tataagccat
    29521 tgccccgaca aatccagcga tgatccctac gaatactccc atattcaaag ctggtgcttc
    29581 caatacactt gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa
    29641 tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag atagccccag tgatccgatt
    29701 gattaaaata aatgagatcc cagctgcaaa tgctccgcct gctctttctt ttgcccaact
    29761 tcctcctatt gctaaagcaa ataaaatatg aagattacca ataattgccc aaccaatatt
    29821 ttcaattact ccgcccgttg tgacaagcgg cgtccaatca ggattgatca agggaattga
    29881 tttccctata ctgatcatca acccagctgc cggcataact gctaccacaa ccattaaagc
    29941 ctttccaaat ttttgccaaa actcaaaact aaatatcttt ttcattccgt ttagctcctt
    30001 tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt tcttttttgt aaatgcttta
    30061 ttttttattt ttctgtctat accattctat aaaacctata atacgtgtgt tcccttctat
    30121 aattttctcc taaaaattta tttttttaag cttttttcta tgaaatattt tcttttgtct
    30181 attgacttgt gcaatcggtt gcgtttatta tgaacgtata aaacagctca ttaggaggag
    30241 aaaaatgaaa cagatcaaac gactatttgc tattgatcca tggaaaatca gcacgacaca
    30301 attagataaa aaaaatcttc gactgcaaga atctttaaca agtattggaa acggttatat
    30361 ggggatgcgc gggaactttg aagaaactta ctcaggggac caccatcaag gtacttattt
    30421 ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg tggaaaaatg gttatccaga
    30481 atactttgga aaagtgatca acgccttgaa ctttatcgcg atagatattt cgatcaacga
    30541 tcagctgatc gacttggctt ctttggaacc agaagacttt tactgggaat tagatatgaa
    30601 aaatggtgta ttatctcgta cattcacaat cactacttct actaataaag tacgcatttc
    30661 cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct tatatctatt taactataga
    30721 aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa
    30781 cgaagacagc aactatgatg aacatttttg gaatgagatt gatcgtggaa ctatagaaga
    30841 aatcggctat ttgacgacta aaactattcc aaacaacttt gggattgaac aatttactgt
    30901 tactgcagca atgcgccatt tcgttaatgg aaaatcagtc atccctgact atcacgaaga
    30961 tactttagca ttacatggtt gcttgtctgc agaattatct caaggtgaga catggtcagt
    31021 ggaaaaagaa attatcgttc ttactagtag agatattcca gaagcgcagc aagcagaaaa
    31081 agctgtctca gaattaacga accagacaaa caattatatc caagcaaaag aagagcaaac
    31141 agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc
    31201 acaacaaggt attcgattca atttgttcca actattctct acttactacg gcgaagatga
    31261 acgattgaat atcggcccca aaggattcac aggagaaaaa tacggcggag ccacttactg
    31321 ggatacagaa gcgtatgctg tccctcttta tcttgcttta gccaaacctg aagtaactaa
    31381 gaatctgctg aaatatcggc ataatcaact gccgcaagca atccacaacg ctcagcaaca
    31441 aggattaaaa ggcgcgcttt atcctatggt caccttcact ggtgtcgaat gccataacga
    31501 gtgggaaatc acattcgagg agattcaccg aaatggcgcg attgcctatg caatctacaa
    31561 ttatacgaat tacaccggcg atacggaata tttgaaaaat gaaggactgg aagttttagc
    31621 agaaatcgca cgcttctggg cagaccgtgt ccatttttca aaacgagcaa ataaatacat
    31681 gatccacggt gtgaccggcc caaacgaata tgagaacaat atcaataaca attggtatac
    31741 aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa aattatttaa cttatcaaaa
    31801 agaagctgct atcactatca gcgaagaaga actagccaaa tggaaagata tcgcagagaa
    31861 catgtacttc ccagttgacg aagaattagg aatctttgtc cagcatgaca cattcttaga
    31921 taaagatctc atgcctgtat ccgatattcc cacaagcgat cttcctctga atcagcattg
    31981 gtcttgggac aaaatcctta gatcttgctt catcaagcag gcagatgtac tacaggggat
    32041 ttatttcttc aatgatactt ttacaaaaga agaaaagcaa aaaaactttg atttctatga
    32101 accaatgact gtccatgagt cttccctttc gccaagtatc cattcgattt tagcagcaga
    32161 attaggaatg gaaagcaaag ctgtagaaat gtaccagcga acagctcgct tggatttgga
    32221 taattacaat aacgacacag aagacggctt gcacatcact tccatgactg gtagctggtt
    32281 gtcgattgtc caaggatttg ctcaaatgaa aactgcccat gatcagttga gattcgcacc
    32341 atttctgcca aaaacttgga aagcttattc tttccatatc aattatcgcg gacgtttatt
    32401 gttcatctca gtcacagatg aacaagttac tttcgagtta ttagaaggtt ctcctctaga
    32461 aatttacgtc tatgaacagt cttattcttt agcggatcgt ctagaaataa cgctaagaaa
    32521 ggaggaagca aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt accgatactg
    32581 ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt acgatcgacc
    32641 acacattcaa cgaacagcta aaaggcgtca gccgagaaga ctcactctct ttgcttttag
    32701 cttatggcgg aaaagaacac tctttttcaa aggaagaatt tgcagaatta gcgaaaagaa
    32761 aaaacgatta ttatttagag atgatccaga cgatcgaacc aaaagatgtc ttccccgggg
    32821 tcgtgccttt actggattct ttaaaagaag aaaaaatcaa gattgctctt gcttctgcaa
    32881 gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac accttacttt gatgctattg
    32941 ccgacccggc agaagcaaca aacggaaaac ctgcgccaga tatcttcctc cttgcagcaa
    33001 aagctgtcgg actaaaagca gaagaatgta tcgggatcga agacgcgcaa gccggtatcc
    33061 aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa gaccttggag
    33121 aggagatccc gcttgtccct gatacgactt ttcttacgat cgattacttg aaagaaaagt
    33181 ggcatgacca tggataatcc agagatctcc gtcgtaccag ttccagagac tgaaataaag
    33241 aaaatcacat tgaagaacga ctacttagaa gtcgttcttc tcaattacgg tgcccgcctg
    33301 caccaaatct tcgcaccaga caaagaagga aaatcagaaa atgttttatt gtcttacgat
    33361 cgtttcgaag atgttctaac agacaaaagc ttttttggag caactgtcgg acctgtagcc
    33421 ggaaggatcc gggatgcttc ttggggtgag catagattgg agaaaaactg cggatcacac
    33481 catatccacg gtggcacgaa aggctggtct ttccaatttt gggaagtaga agttttcaaa
    33541 actccatcat caataggtgt ggtcttttac ttaaaagatg aattttctac ctatccagga
    33601 ccaatcactg ctacgatcac ttaccgttta acaaaaaacg aattagagat gacaacctct
    33661 gctagtagcc ttgttgaaac catctgcaat ccgaccaacc atgcctactt caacctttca
    33721 ggaaatggaa caagagacat ctatgaacat cagttaacgg tctttttaga tggtgtgctt
    33781 gaattagatc aagaaaaact gcctactgga aattggacaa aaaaagaaaa actgccaatc
    33841 gactttagaa aatccccaac actgcaagag atccttgcat gttatccaga tggactagat
    33901 gatgtatttc ttttgcatca tcctaggctt tccaaaactt ccttacaatt attcgagaag
    33961 cattctggtc gccaaatgac tatagcaact tctaataaaa gtatggtatt attttctacg
    34021 actggattcg aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa ctacggatta
    34081 gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg atcgatcaac
    34141 ctccatcctg gtcaagaacg gatcagtcag acgatttatc ggttctctgc acaataacaa
    34201 gagaaaggtg tttttatcat ggctattacc gtaaaagatg tggcgaaaaa agccggtgtt
    34261 gccacctcga ccgtctctcg tgtcatcaac gatcatccta gtatttccga gacaacaaag
    34321 aagaaagtcc gaaaaattat ggatgaacta ggttatgttc ctaacatgac tgcccgcaat
    34381 ctaggaaaac gcatttccag tgccatcggc gtaatcttac cccctcttga ttcaaaagag
    34441 cggatcggta acccatttta tcttgagatc atggaagcaa tcaacgaaga agcgcggaat
    34501 tatgatatga ctacagcaat cgctactgct aaaaattttg aattgctgtt agagaacgtc
    34561 cagcgtatgc atttacaaaa acaagtagat ggttttattc tagtgtattc agatagtcaa
    34621 gatactgtca ttgattattt ataccaaaac cacatccctt tcactttgat cggacagcct
    34681 tatcatcatg agaacgaaat cgtttatgta gataatgata accagcttct aggtaaacaa
    34741 gcaacagaat ttttgatcca gaatggacat aagaatatcc tgtttgtgac aaacacgact
    34801 catgaaagtt tgtatttcga acgatatttc ggttatcaaa aagcaatgat gatggctggt
    34861 cttcctgcgt tatccagtgt agtactagag catccggagg attatctgtc ctttgagaaa
    34921 acgttgaaag aaaacgaagc cacagcattg gtggtcattg acgatatctt cgcccttcgc
    34981 acgatgcagt tggcgcaaat gtatggctat gtcattccag agacactctc cgtcattagc
    35041 ttcaacaact cgatcttctc aacattaaca cacccttacc ttacaagtat cgatatcgat
    35101 atctccgaat taggacgtgt cgctatgcaa aagctgcgcg aattgatcca tgaagaattg
    35161 agcagtggcg tacgtttggt catccctcat cgcttgatca aaagagagac agtcgttcct
    35221 ttagaaaacc catactaaat agctgactca ctaaatacgc tgaccattta tcctatggtc
    35281 agctttttat gtaaaagaag atactacttc taacacattg tttatctgta aaaaagagcg
    35341 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
    35401 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg
    35461 agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tccttccttc
    35521 aattttttac acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa
    35581 gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct aactttcatc gttcccataa
    35641 tgaatgtgcc gctgactccc cacggtacga gagaattgac ggtcgcacct gaatccgcta
    35701 gtgttcgtgt caaataggtt tttgataagc caacgtcatc aaaagaacgc ttgaatgttt
    35761 ctcctggaag aatgatggac agatactgtt ctccaacgat cagattgatt ccgatacagc
    35821 tcaaggcagt aaacccgact aaacgagaag gtcggtccat cttttctttt aattcttgaa
    35881 cgatggtatc aacgatcaga tatttgatca gtaacccacc taatgccaat gcaagtaaaa
    35941 tcaaactagc agaacctaac atactcatga tcccgcctcg agaaagcagt tcatctagct
    36001 ttggatcagg tgtatttgcg acataaccat tcatcaagat gtctgctgct tttccgaccg
    36061 tcaaactttt ctcatggata aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg
    36121 tagggatagc cggaattttt ttgattgccc ccccaaataa aataactagt ggaatcaatg
    36181 tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg atactagcta
    36241 gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg
    36301 ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact cctccaatac
    36361 ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa aaggcaccgg
    36421 aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg atccccaaaa
    36481 aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact ccgacaattg
    36541 cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta aatccataaa
    36601 ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca ccaatcaaaa
    36661 gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg tcatggatct
    36721 catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa gctgcaagga
    36781 tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt gccaacaaca
    36841 cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc gatctcctat
    36901 ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct cctactaaat
    36961 cagcgacaaa acgtcggctg aaagacttat ttttttattt ttcttctaaa cttttttcta
    37021 cctcaatccc ccaagaatct ttcttgaagt gattgatgat caatggaata acgaacatga
    37081 ccactgtgat agctgctact aataatgcat cactcaatgt cgctgtacct aatgtagcaa
    37141 atacgacaac gatgatagag aagatcagca tgcagctgac tacccaagct aatccgtttc
    37201 cgtttttacc aattcggtaa ggacgttcga attctggatg tttcttacgc attttaagca
    37261 atgaaacagc aatcaaaatg tacacgatac aataaataac agttgtagca tttgtaagtg
    37321 tcaagaatac agcacttacg tcatccatta caccataaag caaagcaaat aatgaaataa
    37381 cgattgattg tgttagaacg acatttctag atacgccgta tttgttttct ttatggaatc
    37441 cgaattttgg tggaaggaat ccttcacgag ctacttggat gattgttttt gatggtccag
    37501 taacccaagc tgataattgt aaaagcacac caataaatac catgaagctg aagatatttc
    37561 cgatcacttc tggtaagcct aggatttgac agtcaatcaa gataggctgc gtgatatttg
    37621 ctaattccat ttttccatct ggtacgacat ttgctacaag catcgcattg atgacattca
    37681 ataaaactaa tccgatcaaa gcgataaata cgccttttgt ataatttttt gttgcgtctt
    37741 tcaaacgagg catgtaaaca gaactcattt cgatcccaac aaaaataaat gtgataccag
    37801 ctaagtattt caatgaatca atatgttcca agtcagggaa agcttttgac catgaaaagt
    37861 cacctagata gccgtttgag actagtccga ctttgaaggc tgccaaaaca cctaaaacga
    37921 acatgatgac tactgggata tataccccta gccatacacc gatgttcccg ccgacttttg
    37981 ccatatcgaa tttcaagttc aagatcgtga tgatccagta gataactaaa atacaaccta
    38041 aaacgaacca gtgattattg ccaagttcta cattaccaaa tgtgtttcct aacaatggtc
    38101 ctaaagtaga agcgaccatt accatacctg ggaacatttg cacccataat agccatgcaa
    38161 cgacaaatcc ccatttgctg cccagtgctg tttttaccca taactgagga ccgccttctt
    38221 gaggaagcat cgtggaaagc tcaccggaaa ccattgatat cggaccggca aaaaacaaaa
    38281 ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta ggaatactcc
    38341 gaaccgttgc acacagtgcc attgtcagac cgataaaggt cccaagaccc attttactta
    38401 cttcagattt actcaccgtt gacactctcc ttgatttttt ggataaaata acggtgtctt
    38461 tgaagtagtt tcagcaataa acgaaacaat tcagcgaata tttgagggga ttctttgagt
    38521 ttattgcttg aagggtttct tcaaagacaa cctcaaaaca aagggatcga agagcttagg
    38581 tctccccttg ctcttcagat ccaaacatgc gtcagaaaca gttagtattc aagctgtttt
    38641 ctaacatgat taccttgctt attttgcttc gcttgccaat aattggtctg cgtagatttt
    38701 ttccaatttt tcttgaagag ctgctttgat tttttcagca tattcttcaa agtgttcttc
    38761 tttgttcatg tatggtgtca ttacgctggc acgcaataca gttacttttc cggcacggtt
    38821 ccattcttca tctgagaatc ctagttggtt cacaaattgt aatgggctgt tgccatagtc
    38881 tgggatagca aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac
    38941 gtatgaagaa taatcatata cgtcatggtt caatttattc attgcaacta agtcatcgtt
    39001 gcctttttct ttgaatacat agtctaccat attgaaatct ggataagtta atggatgaac
    39061 ttcgatttct ttgtctccta ctttaaatga caagtcattc aagaagttgt agaaacgatg
    39121 tgcaccttcg attgatgcac ccattaattt accatatcct gtgacattca aaggtaagac
    39181 atggtgtgct gcccaaacgc tggctgctgt tgcccctgct tttgaacctt ccaagatata
    39241 agccccaagt aatgctggga tatctgcgcc tttttcaaat acatatgtcg caaagtaaga
    39301 gataacgtct ctcatgcgga tatctttgat gacgatacca ccggcagaat atggaacata
    39361 gcccatttta tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt tatatgcact
    39421 gtgtacttct tctaagatat aatctttgtt ttctgtaaag acattgtatt tgtaatgaac
    39481 atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc
    39541 gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac
    39601 gcgacgcaat gcaacgattt tatcgatacc gtcgattgct ccttcttctg ttgatcctac
    39661 aacaccgact acacctagga ttggtgtttt ttcagctgct agaccacgaa cgattttttc
    39721 aagttcgttg atgtccatgc gatagttgtg gtcaactggc actggaatca cttggtctaa
    39781 accgataccg atgatgtcgg cagcttttag ccatgaatag tgtttagttt gtggaaccaa
    39841 ccattttcct aatttttcta aatgttttcc gctacgtgct gagtgagctt tgatttcgtc
    39901 gattttttct gggacgctgt ctaacagatt catgatttct tctgttgaca agttcattaa
    39961 ttcccaatcg ctttttccag caactagttc aggtgtcact tctttcattg caagcggcaa
    40021 tgatttgatg ttgcgggcat accaaagtcc ttctaagttt gctaaagaac catcagcaac
    40081 gatgtggccc cagccatctt tatagctcat taattttgca aattccattc ctacttcttc
    40141 ttccatttgg cttgttgcag gagaagattc ataggcaacg ttattaccat tccatagcat
    40201 agcaaaattg tacgctagta aagatggcat caatgtttca gagttcatat gaccccagta
    40261 acgtccggca ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag
    40321 aacatccttt gttttattca cagtgtgttc aaaactagca ctgctttttt cttctggcgt
    40381 aataataggc atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt catccaccaa
    40441 ttcattcaac aaagctttat agatttggcc gttctcagct ttgtccccaa tgaatagggc
    40501 atttaggttt agatctttcg acaatgattc actcatagtg aaaccctcct aaaaaaatat
    40561 ttaaattttg tcgagcccta cattacacct gttcttctat tctgtaaact aagttttggc
    40621 ctgattttta atcatatttt aatatgtatc aattatccga attgttcaca cttttgaaat
    40681 aattaaaaaa aacgagggaa tttacaaatg taaactccct cgcaaagatt gtattaacga
    40741 caaccagcgt tctattttag gatgggtttc aaaattggaa tgttgtaata gtatttccct
    40801 ttttcaaggt aaaaacttac taaattctaa tcaattactt tggccaaaaa gtaatttttc
    40861 ttaccttttc gaataaccac aaacttacca tcgaattcat cggatggatt tatgacaaaa
    40921 tttaaatcgg taacgcggtc accgttaata cttatagctc cgtttgaaac atcttcacgt
    40981 gcttgacgtt tagatggttc aatttttgtg gaaacaagca gttccacgat attttcaggt
    41041 gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc ttccgcattt
    41101 agttctttaa tatttccaga gaacaatgct tgcgttattt tttgcgcttc tttcaagtct
    41161 tcttcactat gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc
    41221 tcaggttctg tctctacttt tttcgctaag tcttcgattt cttcttgaga caagaaagta
    41281 aagaatttca agtatttcac tacatcgcga tcatcttgat tcaaccagaa ttggtagaac
    41341 tcaaatggtg aagttttctt cggatcaagc caaacggcac cgcctgcagt ttttccgaat
    41401 ttcgttccgt cagccttcag catcaatggg atcgttaaac cgaacgcttc tgcttctggt
    41461 ccttctaatt ttcggattag gtctaatcca gctgtgatat ttccccattg gtctgctcca
    41521 ccgatttgca attgcacatc gtatgtttta tgcagatgca aaaagtcgat agattgcagg
    41581 atttggtaag taaactctgt aaatgagatt cctacttcca aacggctggc aacgatatct
    41641 tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa
    41701 gagatttttg acaaccagtc gtagttgttc acaaatgtga tatttgcatc tttaccaaac
    41761 aattttctca tttgattgga aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc
    41821 aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc
    41881 agaatatatg gatgatggcc tgccaattgg aatcttttca tcatcataaa tggaatcaaa
    41941 tgtccgatat gcatcgaatc accagttgga tcaacgccgc aataaagtga aatgctcttt
    42001 tcttcaacga gctcgcgaag tctttcttcg tttgtttgct gattaatggc gtcgcgccaa
    42061 gttaattcat caataatatt cattttactg acctccgtgt agttttttta tgaaagcagg
    42121 aacctgctag catatgtaat taattatttc ttcaggatag gataggaaat aaaaaaagtc
    42181 ccttggcata taagccaagg gacgaaatct cgcggtacca cccaatttgt atgtctagat
    42241 atccaacata cctctcgaac atttaacgct gtcgcgtggc aatcactgcc aaagactcct
    42301 gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg accaccagct ttctgaaaca
    42361 ttggctttaa caactacttc gtttcgatct tcgtcttgtt attattaata tgtgtctatc
    42421 atagagcgca actttttctt tgtcaacaga aaaaggatat tttttttaaa ttcctcattt
    42481 tttctttttt gaatccgctc taattccgaa cattttattt tcaattattg ttaaaagaaa
    42541 taactttttc tgtatttttt ctgcaattta tcgagaagtc actttacttt gcctcttatt
    42601 ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt
    42661 atatggaaaa aaagcaaaaa attattttag gagccacttc tgctttaact atcgttattt
    42721 taattctaag tttgtttttt gtcatccgat catctacaaa aaaagaacca atcattcctg
    42781 aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga taagcaacaa ctagttactc
    42841 aagcaaaaaa tctggctcaa agctacgatt atgaccaagc gattgcgtta ttggaaaaag
    42901 atagtaagaa cggatctact gaaacacaac agcttcttca aacactaaaa aaggagaaag
    42961 aacaactgat cgactggtct gatcctactc agatctctca tgtctttttc cacagcttga
    43021 tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca aggatacaaa gattatatgg
    43081 tgactgttga agaattcaac cgttcgatcg aacaacttta tcaaaatgat tttgttttag
    43141 ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa tctttccttt aacgggcttg
    43201 cgctacctga aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc tattatgaat
    43261 atatggataa ttccggtttt cctagccgat tggtcgtatc aaataacaat gaaatcaaaa
    43321 atgtctataa agagaaagat aaagaagaaa tcggtgactt tgacatggtt cctctgatcg
    43381 atgcctttgt caagaaacat cctgattttt cttatcgagg cgcaaaaggt accttggccc
    43441 tgacaggtta caacggagtt ttaggttatc gaacatcaaa atctcaatat ggagacaatg
    43501 aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc ggaacagttg aagaaagacg
    43561 gctggagctt tgcttcccat acatggggtc acttgaatat gactcaagct tcactagaag
    43621 atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc acctatccta ggaaaaacaa
    43681 atattctgat ttatccattt ggtgcagaca tcagcgactg gcagccttat tcagcagaaa
    43741 atcaaaaatt tgcttacttg aagagtcaag gtttcgatat tttctgtaat gtcgatgctt
    43801 ctacaccagc ttggggacaa ttaggtacgg atttctatcg aaatgctcgg atcaatatcg
    43861 atggcattcg ttttcaatct gatttagacg gaaaaaatcc tattcttgat ccattcatca
    43921 atgtccatga tgtctatgac aaagaagcac gcgaatctgc ttgattttac gtcatttatg
    43981 aaccaaaaaa gccgaactga cattttatcg tcagtttcgg cttttttatg ctttatttct
    44041 tcttcggtgc gattcccttg atcaggactt ctgtccattc tgctgtatat ggaagcagtt
    44101 ttgcgtatat ctccggctta gaaattcctt ctaccattgc ttgaaaatag aggatcaaga
    44161 actcatccga atatttcaaa tccacttttc cttctttccg ccctcgatgg aaaagatcaa
    44221 gcatgatact gaaactttct tgcgtatacg catccatcgc gtcagaaaat tctttttttt
    44281 ctttttttgt atagtatgtc atcaaatcga tataaaattg tttgttcact cgattgagat
    44341 aacggatttt gttttcactc atggcaatca gtgtttcttc aaatggaaga ttttccgcaa
    44401 tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc aaaaaatact tcataaatca
    44461 gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga gcgttctcag
    44521 ctaccttatc cattgtcact ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg
    44581 cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc aaaaccgttc atttgtagag
    44641 cctcccgtaa ttttaaactt ttataatata tat
//
LOCUS       DAZKCE010000010        41656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000010 DAZKCE010000000
VERSION     DAZKCE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.010"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..789)
                     /locus_tag="ACG8KQ_000488"
     CDS             complement(<1..789)
                     /locus_tag="ACG8KQ_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9103458.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIK"
     gene            complement(762..1442)
                     /locus_tag="ACG8KQ_000489"
     CDS             complement(762..1442)
                     /locus_tag="ACG8KQ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9103459.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1465..2403)
                     /locus_tag="ACG8KQ_000490"
     CDS             complement(1465..2403)
                     /locus_tag="ACG8KQ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9103460.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2648..3499)
                     /gene="ispE"
                     /locus_tag="ACG8KQ_000491"
     CDS             complement(2648..3499)
                     /gene="ispE"
                     /locus_tag="ACG8KQ_000491"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9103461.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3653..6226)
                     /gene="mprF"
                     /locus_tag="ACG8KQ_000492"
     CDS             complement(3653..6226)
                     /gene="mprF"
                     /locus_tag="ACG8KQ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9103462.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6367..7173
                     /gene="proC"
                     /locus_tag="ACG8KQ_000493"
     CDS             6367..7173
                     /gene="proC"
                     /locus_tag="ACG8KQ_000493"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9103463.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7252..8808)
                     /gene="rny"
                     /locus_tag="ACG8KQ_000494"
     CDS             complement(7252..8808)
                     /gene="rny"
                     /locus_tag="ACG8KQ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9103464.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9219..10268)
                     /gene="recA"
                     /locus_tag="ACG8KQ_000495"
     CDS             complement(9219..10268)
                     /gene="recA"
                     /locus_tag="ACG8KQ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9103465.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10377..11618)
                     /locus_tag="ACG8KQ_000496"
     CDS             complement(10377..11618)
                     /locus_tag="ACG8KQ_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9103466.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11858..12436)
                     /gene="pgsA"
                     /locus_tag="ACG8KQ_000497"
     CDS             complement(11858..12436)
                     /gene="pgsA"
                     /locus_tag="ACG8KQ_000497"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9103467.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12645..13568)
                     /locus_tag="ACG8KQ_000498"
     CDS             complement(12645..13568)
                     /locus_tag="ACG8KQ_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9103468.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14307..15593)
                     /locus_tag="ACG8KQ_000499"
     CDS             complement(14307..15593)
                     /locus_tag="ACG8KQ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9103469.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15586..16854)
                     /locus_tag="ACG8KQ_000500"
     CDS             complement(15586..16854)
                     /locus_tag="ACG8KQ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9103470.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17106..17567
                     /gene="mscL"
                     /locus_tag="ACG8KQ_000501"
     CDS             17106..17567
                     /gene="mscL"
                     /locus_tag="ACG8KQ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9103471.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17626..18072)
                     /locus_tag="ACG8KQ_000502"
     CDS             complement(17626..18072)
                     /locus_tag="ACG8KQ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9103472.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18165..18410)
                     /locus_tag="ACG8KQ_000503"
     CDS             complement(18165..18410)
                     /locus_tag="ACG8KQ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9103473.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18525..19103)
                     /locus_tag="ACG8KQ_000504"
     CDS             complement(18525..19103)
                     /locus_tag="ACG8KQ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9103474.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19180..19710)
                     /locus_tag="ACG8KQ_000505"
     CDS             complement(19180..19710)
                     /locus_tag="ACG8KQ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9103475.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19714..20574)
                     /gene="rlmB"
                     /locus_tag="ACG8KQ_000506"
     CDS             complement(19714..20574)
                     /gene="rlmB"
                     /locus_tag="ACG8KQ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9103476.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20567..20962)
                     /locus_tag="ACG8KQ_000507"
     CDS             complement(20567..20962)
                     /locus_tag="ACG8KQ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9103477.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20959..22371)
                     /gene="cysS"
                     /locus_tag="ACG8KQ_000508"
     CDS             complement(20959..22371)
                     /gene="cysS"
                     /locus_tag="ACG8KQ_000508"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9103478.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22368..22904)
                     /gene="epsC"
                     /locus_tag="ACG8KQ_000509"
     CDS             complement(22368..22904)
                     /gene="epsC"
                     /locus_tag="ACG8KQ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9103479.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(22990..23229)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23226..24686)
                     /gene="gltX"
                     /locus_tag="ACG8KQ_000510"
     CDS             complement(23226..24686)
                     /gene="gltX"
                     /locus_tag="ACG8KQ_000510"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9103480.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24856..26013)
                     /locus_tag="ACG8KQ_000511"
     CDS             complement(24856..26013)
                     /locus_tag="ACG8KQ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9103481.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26047..27417)
                     /gene="radA"
                     /locus_tag="ACG8KQ_000512"
     CDS             complement(26047..27417)
                     /gene="radA"
                     /locus_tag="ACG8KQ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9103482.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27480..28007)
                     /locus_tag="ACG8KQ_000513"
     CDS             complement(27480..28007)
                     /locus_tag="ACG8KQ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9103483.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28237..29043
                     /gene="proB"
                     /locus_tag="ACG8KQ_000514"
     CDS             28237..29043
                     /gene="proB"
                     /locus_tag="ACG8KQ_000514"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9103484.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29040..30287
                     /locus_tag="ACG8KQ_000515"
     CDS             29040..30287
                     /locus_tag="ACG8KQ_000515"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9103485.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30363..30707
                     /locus_tag="ACG8KQ_000516"
     CDS             30363..30707
                     /locus_tag="ACG8KQ_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103486.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30824..31150)
                     /locus_tag="ACG8KQ_000517"
     CDS             complement(30824..31150)
                     /locus_tag="ACG8KQ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103487.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31765..32967)
                     /locus_tag="ACG8KQ_000518"
     CDS             complement(31765..32967)
                     /locus_tag="ACG8KQ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9103488.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33084..33599
                     /locus_tag="ACG8KQ_000519"
     CDS             33084..33599
                     /locus_tag="ACG8KQ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9103489.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33798..34244)
                     /locus_tag="ACG8KQ_000520"
     CDS             complement(33798..34244)
                     /locus_tag="ACG8KQ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9103490.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34291..35769)
                     /gene="msr(C)"
                     /locus_tag="ACG8KQ_000521"
     CDS             complement(34291..35769)
                     /gene="msr(C)"
                     /locus_tag="ACG8KQ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9103491.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36123..36752)
                     /locus_tag="ACG8KQ_000522"
     CDS             complement(36123..36752)
                     /locus_tag="ACG8KQ_000522"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9103492.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36736..37200)
                     /locus_tag="ACG8KQ_000523"
     CDS             complement(36736..37200)
                     /locus_tag="ACG8KQ_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9103493.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37687..38955)
                     /locus_tag="ACG8KQ_000524"
     CDS             complement(37687..38955)
                     /locus_tag="ACG8KQ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9103494.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39186..39923)
                     /locus_tag="ACG8KQ_000525"
     CDS             complement(39186..39923)
                     /locus_tag="ACG8KQ_000525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9103495.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(39990..40559)
                     /locus_tag="ACG8KQ_000526"
     CDS             complement(39990..40559)
                     /locus_tag="ACG8KQ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9103496.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40687..41559
                     /locus_tag="ACG8KQ_000527"
     CDS             40687..41559
                     /locus_tag="ACG8KQ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9103497.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT        11799 a         8987 c         7427 g        13443 t
ORIGIN      
        1 tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg
       61 aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca
      121 aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg
      181 aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc
      241 cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg
      301 ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat
      361 accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat
      421 cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa
      481 tactaacgct agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt
      541 tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt
      601 cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg
      661 agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg
      721 ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag
      781 caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg
      841 atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt
      901 cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca
      961 agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt
     1021 ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa
     1081 gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga
     1141 tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca
     1201 acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc
     1261 tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg
     1321 gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca
     1381 tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc
     1441 accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt
     1501 ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa
     1561 aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga
     1621 agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag
     1681 ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg
     1741 ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg
     1801 gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat
     1861 ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc
     1921 ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc
     1981 gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga
     2041 ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga
     2101 atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc
     2161 atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc
     2221 agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat
     2281 cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt
     2341 tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt
     2401 catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa
     2461 tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta
     2521 tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca
     2581 ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc
     2641 ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat
     2701 tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc
     2761 cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga
     2821 ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct
     2881 gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt
     2941 gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca
     3001 caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa
     3061 atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg
     3121 ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg
     3181 cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga
     3241 tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat
     3301 tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg
     3361 ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact
     3421 catgataccc atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg
     3481 ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat
     3541 catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc
     3601 aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt
     3661 ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga
     3721 gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt
     3781 cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat
     3841 taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc
     3901 caacattagc taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata
     3961 caataaacag attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc
     4021 gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga
     4081 ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc
     4141 gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta
     4201 cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca
     4261 tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa
     4321 caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt
     4381 agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa
     4441 actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc
     4501 atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat
     4561 aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt
     4621 ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag
     4681 ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg
     4741 agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta
     4801 aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc
     4861 tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc
     4921 tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg
     4981 tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct
     5041 ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat
     5101 tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga
     5161 ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag
     5221 cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa
     5281 atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta
     5341 gaacataata aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag
     5401 acaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca
     5461 tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa
     5521 tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc
     5581 caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct
     5641 ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc
     5701 agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa
     5761 gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta
     5821 aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca
     5881 caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact
     5941 cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat
     6001 acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc
     6061 ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg
     6121 cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac
     6181 tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct
     6241 aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt
     6301 tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag
     6361 gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt
     6421 ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa
     6481 aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt
     6541 gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg
     6601 gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca
     6661 ttaatagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg
     6721 aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg
     6781 gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta
     6841 gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac
     6901 atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc
     6961 actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa
     7021 aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta
     7081 gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc
     7141 gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac
     7201 aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc
     7261 ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag
     7321 ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat
     7381 ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc
     7441 tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc
     7501 taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag
     7561 gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac
     7621 tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc
     7681 aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg
     7741 gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg
     7801 cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag
     7861 atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat
     7921 acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat
     7981 tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc
     8041 gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag
     8101 cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc
     8161 attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg
     8221 aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc
     8281 agattctttt accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat
     8341 gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc
     8401 ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag
     8461 tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt
     8521 cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc
     8581 tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa
     8641 tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga
     8701 tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc
     8761 aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc
     8821 catactatct ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc
     8881 gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt
     8941 tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa
     9001 cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa attgagaaac
     9061 ttttctatta aattattctt tgtagatgaa cagatatttt tattttatgt acccactcgt
     9121 ataattaaat aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac
     9181 ctactcttca gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat
     9241 tcttcttgtc ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct
     9301 ctaactagag cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg
     9361 ttttcccgtc cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca
     9421 acaatgtctt tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca
     9481 tacatgacat ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg
     9541 atctttgtac ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact
     9601 tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc
     9661 gcaaacatca cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt
     9721 ttgttgatcg aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg
     9781 acatgtgagt cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa
     9841 tcgactacta cgatatctac agccccactt gatactaaag catcagcgat ctctagacct
     9901 tgttctcctg tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt
     9961 gcatattgcg gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt
    10021 tgtacttctg caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat
    10081 acttcgatga tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga
    10141 gagccacttg gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag
    10201 cctttaccat agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca
    10261 tctgccaata cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta
    10321 tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat
    10381 tttcttaaaa gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata
    10441 gcagcacgat ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata
    10501 gcgatccata ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg
    10561 aaagctacac tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca
    10621 cattctttgc tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca
    10681 gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca
    10741 atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc
    10801 tgcttgtttt ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat
    10861 ccataaaaat attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga
    10921 ttccctgcct gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg
    10981 gcatatggtg cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc
    11041 agtcgcgatt caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa
    11101 ggaaaacgtt cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta
    11161 ggcggaccgg gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca
    11221 agccctgtac gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg
    11281 tttttggtca tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc
    11341 gtattttgta tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct
    11401 tctgtcggtc ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca
    11461 atcaactctt ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata
    11521 cctagatcag ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt
    11581 aaaagttctg ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag
    11641 tatacgctgt atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat
    11701 cttgtcacag ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag
    11761 tcacaatata cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt
    11821 tagacggcag gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac
    11881 cgctgttttt gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa
    11941 gcatgatttg atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca
    12001 agataatagc aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga
    12061 cttctccatg ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa
    12121 tgatcgctac tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca
    12181 taactagcat tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc
    12241 gcgcacgggc aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg
    12301 cacctactag ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta
    12361 aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt
    12421 tatttggtaa attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa
    12481 gtttccaccg atattattta cttttccaaa tgaatttatt caaaggagta tttttattga
    12541 aattgatctt tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt
    12601 ttttatacct acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga
    12661 agagtaaggt tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat
    12721 tctccattcg ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt
    12781 gctgtttccg gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca
    12841 taggcattgt ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt
    12901 acaggcttgg tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta
    12961 gctattgcca ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct
    13021 ttggtctgcg tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc
    13081 gaagaagctg tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc
    13141 acgacaacga caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa
    13201 aagcgacttg gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca
    13261 ggctgcggac gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact
    13321 aaatggtctc catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa
    13381 aatttaccag gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga
    13441 atttttgtaa tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt
    13501 aatttttctc cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc
    13561 cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt
    13621 cacagctcct tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac
    13681 aatatccaat aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa
    13741 cggcaggctc acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct
    13801 caaaaaccgg caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa
    13861 aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
    13921 agtccaacca agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga
    13981 tgcagggcag ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc
    14041 aataagccga aatatcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    14101 tgaagctgac cacttctgtc acaacctctt tgattacttc gtttgatttt attttaatat
    14161 aagttgatta aaagagagca tgatttgttt tattttctca ctaatttttc caacacaact
    14221 aagaatacca taaaagctga ttttggagga ggttcctact acaagtctaa aacgaaattt
    14281 aagacttttc ctcataaaat actggtttat tttggtctca tgtaaaactc gctgaaggcc
    14341 tcttcactta taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg
    14401 atttcaggaa gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca
    14461 atatattcga tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa
    14521 tcgagatttg tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact
    14581 ttttctgctg ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca
    14641 cgatccaagc tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct
    14701 agatagtttc gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta
    14761 tagcgtaaca gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca
    14821 aatttatctc gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt
    14881 tcagggaaat aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc
    14941 cgaataagtt tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg
    15001 cttggctgat aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata
    15061 ctttcaaccg tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt
    15121 atgccaaaaa acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg
    15181 attccttttt ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc
    15241 agagtcgtca gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta
    15301 aaacttgtga aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca
    15361 tcttcttttt caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc
    15421 ttttcttttt caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac
    15481 aaaccatatg tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc
    15541 agtacttcat gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt
    15601 tcctcctgct aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc
    15661 tttgatcgtt acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg
    15721 cagccagctg tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag
    15781 atattggttt ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt
    15841 tcgcaaactt tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc
    15901 gataccggtt tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata
    15961 agctagactt tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa
    16021 taaaccgtta aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg
    16081 ataagctagg ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat
    16141 attcacttga ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg
    16201 taattgccca actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc
    16261 gatttgatca tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt
    16321 gtccaaatct tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata
    16381 aagttcttgc aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata
    16441 tagacgcaga ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat
    16501 atttggataa aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc
    16561 attgacatat tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt
    16621 ttttctaact gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt
    16681 tgtctgatcc gaataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt
    16741 cgtgatgact tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta
    16801 ttgttttgtt ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat
    16861 atgctccttt tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt
    16921 acttattcct ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg
    16981 ttacacttta tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt
    17041 atattgtttt tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt
    17101 cattcatgat caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg
    17161 tcggcgttgt tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga
    17221 tcacaccact aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag
    17281 cactttcaat tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta
    17341 ttagtgctat cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg
    17401 caaaccgtgc gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta
    17461 ctgcagaaga ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca
    17521 atacacatcc agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa
    17581 aaaaagaaag ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg
    17641 ttcctttttc atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt
    17701 gaatccaaac gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt
    17761 ttctggaaaa tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc
    17821 atctttacgg ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc
    17881 atatactcgc gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc
    17941 ttctccatca atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc
    18001 gattcgttct ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac
    18061 ctgatattcc actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca
    18121 caaaaaagca aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt
    18181 aaaaattcga tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca
    18241 aaagaatttt cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa
    18301 acgccgcggc gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg
    18361 ccgatacgac cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc
    18421 tctactatca actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga
    18481 aatgcaataa aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa
    18541 aatttcaagt gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg
    18601 tgatccattg ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga
    18661 aaaattttag cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat
    18721 aaaagatgct gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct
    18781 tctgtctcta ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg
    18841 ttctgcttcc tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca
    18901 aagctaacac ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct
    18961 tcttgttccc aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt
    19021 aaaagtggaa aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca
    19081 gcgcgcaata actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc
    19141 ttatcatcaa tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa
    19201 atcgtccaat ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg
    19261 gaaatcaata aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat
    19321 cgtcttatat aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg
    19381 ttctgctaaa tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc
    19441 gattctctcg atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg
    19501 gtatttctta taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac
    19561 ttccattcct tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc
    19621 cgccattttg tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta
    19681 gccgtctaca atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc
    19741 ctgatacata agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat
    19801 cgggatcgtc agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc
    19861 attaccaatg actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt
    19921 catatctgtg ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa
    19981 attcgtcacg cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat
    20041 aggtgtgatt ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc
    20101 tgctgtccgt aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa
    20161 gaaggggtct tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc
    20221 ataaggtgta atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa
    20281 tttttgttta ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc
    20341 ttctactttt tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg
    20401 tagcgcttct actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt
    20461 ttgatcagaa actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt
    20521 tttgtcttca aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt
    20581 caccaacttt ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca
    20641 aatgcagata gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag
    20701 cattttttgc gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt
    20761 cttctgtcaa aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag
    20821 aaacataatt cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca
    20881 ggtggtcacg aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt
    20941 tcaattgtgt gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc
    21001 taataagatc cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg
    21061 attgcttctt gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc
    21121 ttctgctgta aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg
    21181 tgccaagaca gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag
    21241 ctcatagacc actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa
    21301 tcgctgttct aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga
    21361 ggcagtttct tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt
    21421 ggctgcttct ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc
    21481 catgaagaaa cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa
    21541 attccctaga gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg
    21601 catccagtaa ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc
    21661 atgatgagga aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg
    21721 ttttgttgcc ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga
    21781 atcccaagaa atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc
    21841 tttttgctgc tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga
    21901 ttggtcactt aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga
    21961 agattcataa gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat
    22021 atgatccatg actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc
    22081 ttcaaatgct cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc
    22141 tgtacggatg attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc
    22201 tcgatattca aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat
    22261 atggatataa ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat
    22321 agggatgaat tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc
    22381 ctcacttttt ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg
    22441 tctggaacat ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt
    22501 accggtccta atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt
    22561 cttttacccg tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg
    22621 tcctcgattt ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca
    22681 gttccaatcg ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca
    22741 ttgatcttcg ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa
    22801 aacaaggcat gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga
    22861 tcgtttctct ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt
    22921 cgtaagaatt ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa
    22981 gtttccttat caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat
    23041 ctctgacagc taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt
    23101 tcctgttttc tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga
    23161 taccttgcac cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat
    23221 cgtgtttagt tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg
    23281 atcgtttcag gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg
    23341 aaaagatttt ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca
    23401 gttggagcat ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta
    23461 ggaactgttt caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg
    23521 aaaaatagcg tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg
    23581 ctaacgatct tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca
    23641 tcagcgatta gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg
    23701 taatggttgt ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg
    23761 cgttttggat cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca
    23821 ggtgaccagc ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg
    23881 cgatattgtt cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt
    23941 tcactgttga tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca
    24001 tagatcatca gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg
    24061 attttcatca aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt
    24121 ttttggatga caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc
    24181 atatctgtaa atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga
    24241 ccttgtgctt ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa
    24301 tgaggcattt ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg
    24361 caatagcatt tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt
    24421 tctttacgtt cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc
    24481 caattcattc ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta
    24541 cgtttttggt cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac
    24601 aaataattga ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt
    24661 gcatagcgta cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta
    24721 acagcaaaaa ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca
    24781 caaattttac cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt
    24841 ggctgaattt cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc
    24901 gattcctcgt cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag
    24961 ggcacttgtt acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac
    25021 aaccatcgtg ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac
    25081 tactagaaca ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc
    25141 attgatatta agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt
    25201 ggttacaatg acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact
    25261 gatatcttca aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc
    25321 atttaggata tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc
    25381 gatgtattgt agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc
    25441 agtctttgta atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata
    25501 tttatggaaa ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt
    25561 ttcttgctcg atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt
    25621 cgttcccatt ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact
    25681 attgacgaat ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa
    25741 tccgatgatc gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt
    25801 ttgttctact tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa
    25861 gaaaataagt gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg
    25921 ttgaccgatt gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat
    25981 gatcatcagc aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt
    26041 tattagttag cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc
    26101 gattcctttt ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa
    26161 gccgagtttt tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc
    26221 agtcagtcca atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct
    26281 tgaagcaata ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc
    26341 tgcagctttt aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac
    26401 tgccatgatc agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc
    26461 gaacatagta ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat
    26521 tgccacaacg atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga
    26581 aggattagcg acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt
    26641 tgagccaaat cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc
    26701 aaaatataac accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc
    26761 tttggtaaca tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag
    26821 ttctgcagtg gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg
    26881 cgtcatcgtt tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg
    26941 actgatttcg ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag
    27001 gcgttccgcg cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt
    27061 tccgcctatg gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc
    27121 tggatcgcca ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt
    27181 cagttcttct aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt
    27241 tgttggtttt gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg
    27301 ttcgatttct tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt
    27361 aggcgacaca tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt
    27421 accgcctcct tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt
    27481 tatttactgg aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct
    27541 tttaaaaacg gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc
    27601 ttgaaaccaa agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa
    27661 taatcactgt caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg
    27721 cttgaacggt tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta
    27781 ggcacgaggg ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct
    27841 gcgactgttt ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct
    27901 tctgctgctt caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata
    27961 ttttgatctt tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcctccc
    28021 taaataaact actcctattc taacatatcc gaggctgtaa aagactttct ttacaaattc
    28081 ttcaagaaat tctgctatct taaagtaaac cattaattcg ttctttttcc aaacgactgc
    28141 aagttgtaca aaaaaactat tttttacatg actttactcg tcaaaacaag tagaatagaa
    28201 cgaaattaat gagtggatat ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca
    28261 aaacgaatta ttgtcaaagt aggaacaagt acattgattt atcccaatgg aaatatcaat
    28321 cttgcggcaa tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa
    28381 gtgatcttag tctcatcagg agctgtaggc gttggtatgc ataaattaca tttgtcagaa
    28441 cgtccgactt tgattcctga acagcaagct gcggcggctg ttggtcaagc cgaactgatg
    28501 aatatctata atcaaagatt cagtgtatat aatcaacaga cggcacagat cctcgtgaca
    28561 agagacgtgg tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt
    28621 ttgattatgg acacgatccc tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta
    28681 gatcatctga caaaatttgg cgataatgat cagctgtcag ccatcatggc aaaacttgtc
    28741 gcggcagacc ttttgatcgt cctttctgat atcgacgggt tttattcaga taatccgact
    28801 acaaatcccg aggctcagat gtatcgctat atcgataaaa tcgatgaaaa gattctggca
    28861 caagcaggtg gtgctggtag cacatttgga acaggcggta tgcagagtaa attaaaagcc
    28921 gcttctagga tatttgaagc aaacagcgca atgattttag caaatggaaa aaatccggca
    28981 attatttttg atattcttgc aggaaaagaa gtaggtactt tatttaagga ggacttgaaa
    29041 tgactgactt gatgcaatta ggacaacagg caaaagatag cgcgcaacaa ctagcactta
    29101 tgcctacaaa ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc aaaaagaatc
    29161 aacaagcgat cattgaagca aatcataaag acttagaaaa agcagtcgaa aacaatatca
    29221 gtgaaacgat gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc atggctacag
    29281 agatcgaaaa aattgcttcg ttagacgatc ctattggaaa agtagatgag atgtggacga
    29341 acaccgacgg attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt
    29401 acgaatctcg tccaaatgtc acgacagatg cagctagtct agcattcaaa tcagggaatg
    29461 ccgttatcct tcgcggtggc aaagaagcat tcttctcgaa tcaattatta gtccaattat
    29521 tgcagcaagt actggtcagt gctggcgaat ctccttatgc catccagttt gtcgatgata
    29581 cttctcatga aactgcagga cagttgatgc gattgacaga atacttagat gtgctgattc
    29641 ctcgaggcgg agcaaaatta atccaacgag tcaaagaaca ggcaacggta cctatcattg
    29701 aaacaggtac aggaaataat cacgtatata tcgataaaga agcacagtta tctatggcag
    29761 tgaacatcat cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat
    29821 tgctgatcca ttctgagatc gcaccttttt tcttgccagc tattgaaaaa gaactggtag
    29881 aacacggggt ctctttacgt gcggatgctc gtgcgttaga atatctagag acggctgctt
    29941 tagcagaaga cacagattgg gatactgaat atctagatta tatccttgct gtcaaagtgg
    30001 tcgattcttt ggatgaagca attgcacata tcaatcggca taatactaaa cattcagaaa
    30061 cgatcgtgac agatagttat gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg
    30121 ctgtctatgt caatgcctcc actcgtttta cagacggatc ggtatttggt tttggtgcag
    30181 agattggtat ctctactcaa aagttgcatg cccgtggtcc aatggggtta aatgaattaa
    30241 cttccacaaa atatatcatt tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa
    30301 acattattcc cgccttttgc cttttttctg taaaggttct atccaactag aaaggtgtca
    30361 atatgtttga aattttaatg atttttttta tttaccttat ttctctgaat atcgctgctt
    30421 ttctgggagt cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc
    30481 aacgggaaaa atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct
    30541 tgcacattag ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt
    30601 tcgatcattc aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt
    30661 ataaatatca gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac
    30721 gataagtaga gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca
    30781 aagattttgt cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta
    30841 aatctgtttc ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac
    30901 gaagcagctc atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag
    30961 ccagtagctg ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc
    31021 tagtttcacc ttccgcattg attattggca attctttgat atagccacga taaatcaacc
    31081 gatcatctga aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt
    31141 cttccagcat ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga
    31201 aaattttcca gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat
    31261 cccctttttc cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt
    31321 caacaaacat gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag
    31381 tctcacaacc tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    31441 cggtaaaata tgagaagcta tttttccgtt ttgtttctct aatacttagg ataaaacgct
    31501 gattttacaa cctctgttaa acggtgttca atcagctgtc cttcggtatt agctcaaaaa
    31561 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg acaaaacgct
    31621 gattttacaa cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    31681 ccaaacgaaa aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac
    31741 agctttttca caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg
    31801 ataaatgata caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct
    31861 ttcaccaata tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa
    31921 gcggacaaaa ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc
    31981 aatcgtcgtc aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat
    32041 cggtacaagg actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa
    32101 gacaaatgct gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac
    32161 gaaaatcgaa gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa
    32221 ttcagaatag ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt
    32281 atataacaat gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc
    32341 ttcccaaagg cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc
    32401 tataaaaccg caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact
    32461 gcccaagacc ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata
    32521 catcatgatt gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt
    32581 agagacaaat aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat
    32641 attcccagat aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa
    32701 tgtttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt
    32761 gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc
    32821 agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt
    32881 agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat
    32941 atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta
    33001 ttttacatgt caatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa
    33061 ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt
    33121 tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc
    33181 atcacagtca gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt
    33241 tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc
    33301 ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag
    33361 caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta
    33421 gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc
    33481 tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc
    33541 agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag
    33601 ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag
    33661 caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc
    33721 actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt
    33781 caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc
    33841 gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc
    33901 atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac
    33961 atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg
    34021 ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag
    34081 acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa
    34141 tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac
    34201 atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt
    34261 aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat
    34321 tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg
    34381 agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt
    34441 tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa
    34501 aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt
    34561 caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa
    34621 ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt
    34681 ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg
    34741 gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt
    34801 acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg
    34861 gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa
    34921 acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc
    34981 taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga
    35041 caaccgatcc agtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc
    35101 cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga
    35161 ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata
    35221 attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc
    35281 atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc
    35341 ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat
    35401 cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct
    35461 ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt
    35521 taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt
    35581 ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt
    35641 accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc
    35701 aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa
    35761 attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa
    35821 gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat
    35881 aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa
    35941 aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt
    36001 gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt
    36061 tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc
    36121 ttttattcga acatagtgat cttttctggt gctaaacgaa cagactcgta accttcttct
    36181 accgcttttc ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat
    36241 gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca
    36301 cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc
    36361 atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg
    36421 acttcttttg gcatcttccc tgattcgtaa gcttggttgt agatttcttc gccatattcg
    36481 taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta
    36541 ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag
    36601 cgccatggtt tcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc
    36661 atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc
    36721 ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt
    36781 tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac
    36841 atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctt
    36901 gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt
    36961 tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc
    37021 ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag
    37081 tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac
    37141 gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat
    37201 aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa
    37261 aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat
    37321 agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa
    37381 aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag tagataattt
    37441 tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt
    37501 ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt
    37561 tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca
    37621 tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac
    37681 ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca
    37741 atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa
    37801 tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc
    37861 gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta
    37921 aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc
    37981 atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg
    38041 ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc
    38101 atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc
    38161 agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt
    38221 ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac
    38281 gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat
    38341 ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc
    38401 tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact
    38461 ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact
    38521 ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc
    38581 ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt
    38641 caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg
    38701 agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc
    38761 ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtacttt ctgtatcgtt
    38821 ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc
    38881 agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa
    38941 attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt
    39001 aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttaat
    39061 aaatttgctt tttctgcata aaaagacgta aatgaagaat attcttctcc attcacgcct
    39121 taaattgatg gtcgtttgaa taactattta acaagtaatt ctctctggat attatccgtt
    39181 atggattata cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga catccggtct
    39241 ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cagatgttaa tccccattct
    39301 ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca
    39361 gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga
    39421 cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca
    39481 ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt
    39541 tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc
    39601 atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca
    39661 ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg
    39721 acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat
    39781 tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt
    39841 tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc
    39901 actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat
    39961 actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt
    40021 ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc
    40081 gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg
    40141 atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga
    40201 tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct
    40261 aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct
    40321 atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct
    40381 actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca
    40441 gtattcagcg ctgctaaagg ctcttcgttt tccgcttgga tctgatggat cacttcaaag
    40501 agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt
    40561 ttgctccctc cttttatcct tactttataa cggacggctt atgatgtcta atacttataa
    40621 aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg
    40681 gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc
    40741 agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat
    40801 ttagaagaag aacttggtgt gacacttttc actcgaggct cccgagagat acagttgacg
    40861 gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg
    40921 gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa
    40981 actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat
    41041 atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga
    41101 ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga
    41161 ctgccagccc aagatcgttg gggacttttg atacatcgga atcatccttt agcaaagcaa
    41221 acagtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca
    41281 gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga
    41341 acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta
    41401 gctctagatg ggatcatcaa tacttatggg acggaaaaag tattcatccc actcgatccg
    41461 ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct
    41521 cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct
    41581 ttcagctttt taacaacgaa taagttaagc aaaagatcca atgattaaat aacataagtt
    41641 tcttaatttg ttcctt
//
LOCUS       DAZKCE010000011        36606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000011 DAZKCE010000000
VERSION     DAZKCE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.011"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1729)
                     /locus_tag="ACG8KQ_000528"
                     /pseudo
     CDS             complement(94..1729)
                     /locus_tag="ACG8KQ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2052..2531)
                     /gene="rlmH"
                     /locus_tag="ACG8KQ_000529"
     CDS             complement(2052..2531)
                     /gene="rlmH"
                     /locus_tag="ACG8KQ_000529"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9103498.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2959..4248
                     /locus_tag="ACG8KQ_000530"
     CDS             2959..4248
                     /locus_tag="ACG8KQ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9103499.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4287..4892)
                     /gene="ytpR"
                     /locus_tag="ACG8KQ_000531"
     CDS             complement(4287..4892)
                     /gene="ytpR"
                     /locus_tag="ACG8KQ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9103500.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5097..5561)
                     /locus_tag="ACG8KQ_000532"
     CDS             complement(5097..5561)
                     /locus_tag="ACG8KQ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9103501.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5583..5903)
                     /locus_tag="ACG8KQ_000533"
     CDS             complement(5583..5903)
                     /locus_tag="ACG8KQ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9103502.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6047..7126)
                     /gene="pepA"
                     /locus_tag="ACG8KQ_000534"
     CDS             complement(6047..7126)
                     /gene="pepA"
                     /locus_tag="ACG8KQ_000534"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9103503.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7326..7676
                     /locus_tag="ACG8KQ_000535"
     CDS             7326..7676
                     /locus_tag="ACG8KQ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9103504.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7899..8846
                     /locus_tag="ACG8KQ_000536"
     CDS             7899..8846
                     /locus_tag="ACG8KQ_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9103505.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8903..10321)
                     /gene="pepV"
                     /locus_tag="ACG8KQ_000537"
     CDS             complement(8903..10321)
                     /gene="pepV"
                     /locus_tag="ACG8KQ_000537"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9103506.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10470..11315
                     /locus_tag="ACG8KQ_000538"
     CDS             10470..11315
                     /locus_tag="ACG8KQ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9103507.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11319..11759
                     /locus_tag="ACG8KQ_000539"
     CDS             11319..11759
                     /locus_tag="ACG8KQ_000539"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9103508.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11809..12003
                     /locus_tag="ACG8KQ_000540"
     CDS             11809..12003
                     /locus_tag="ACG8KQ_000540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103509.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12139..12825
                     /locus_tag="ACG8KQ_000541"
     CDS             12139..12825
                     /locus_tag="ACG8KQ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9103510.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13233..13922)
                     /locus_tag="ACG8KQ_000542"
     CDS             complement(13233..13922)
                     /locus_tag="ACG8KQ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103511.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13897..16026)
                     /locus_tag="ACG8KQ_000543"
     CDS             complement(13897..16026)
                     /locus_tag="ACG8KQ_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9103512.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16074..17411)
                     /locus_tag="ACG8KQ_000544"
     CDS             complement(16074..17411)
                     /locus_tag="ACG8KQ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9103513.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17663..18022)
                     /gene="rplT"
                     /locus_tag="ACG8KQ_000545"
     CDS             complement(17663..18022)
                     /gene="rplT"
                     /locus_tag="ACG8KQ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9103514.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18075..18275)
                     /gene="rpmI"
                     /locus_tag="ACG8KQ_000546"
     CDS             complement(18075..18275)
                     /gene="rpmI"
                     /locus_tag="ACG8KQ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9103515.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18347..18871)
                     /gene="infC"
                     /locus_tag="ACG8KQ_000547"
     CDS             complement(18347..18871)
                     /gene="infC"
                     /locus_tag="ACG8KQ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9103516.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18915..19040)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19191..19823
                     /locus_tag="ACG8KQ_000548"
     CDS             19191..19823
                     /locus_tag="ACG8KQ_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9103517.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19825..20730)
                     /locus_tag="ACG8KQ_000549"
     CDS             complement(19825..20730)
                     /locus_tag="ACG8KQ_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9103518.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20815..21474)
                     /locus_tag="ACG8KQ_000550"
     CDS             complement(20815..21474)
                     /locus_tag="ACG8KQ_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9103519.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21790..22734
                     /gene="mvk"
                     /locus_tag="ACG8KQ_000551"
     CDS             21790..22734
                     /gene="mvk"
                     /locus_tag="ACG8KQ_000551"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9103520.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22727..23704
                     /gene="mvaD"
                     /locus_tag="ACG8KQ_000552"
     CDS             22727..23704
                     /gene="mvaD"
                     /locus_tag="ACG8KQ_000552"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9103521.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23717..24802
                     /locus_tag="ACG8KQ_000553"
     CDS             23717..24802
                     /locus_tag="ACG8KQ_000553"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9103522.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24799..25863
                     /gene="fni"
                     /locus_tag="ACG8KQ_000554"
     CDS             24799..25863
                     /gene="fni"
                     /locus_tag="ACG8KQ_000554"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9103523.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26112..26915)
                     /locus_tag="ACG8KQ_000555"
     CDS             complement(26112..26915)
                     /locus_tag="ACG8KQ_000555"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9103524.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26935..27777)
                     /gene="kduI"
                     /locus_tag="ACG8KQ_000556"
     CDS             complement(26935..27777)
                     /gene="kduI"
                     /locus_tag="ACG8KQ_000556"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9103525.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27793..28800)
                     /locus_tag="ACG8KQ_000557"
     CDS             complement(27793..28800)
                     /locus_tag="ACG8KQ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9103526.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28807..29451)
                     /locus_tag="ACG8KQ_000558"
     CDS             complement(28807..29451)
                     /locus_tag="ACG8KQ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9103527.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29551..30309)
                     /locus_tag="ACG8KQ_000559"
     CDS             complement(29551..30309)
                     /locus_tag="ACG8KQ_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9103528.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30436..31587)
                     /gene="fucO"
                     /locus_tag="ACG8KQ_000560"
     CDS             complement(30436..31587)
                     /gene="fucO"
                     /locus_tag="ACG8KQ_000560"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9103529.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31669..31980)
                     /gene="rhaM"
                     /locus_tag="ACG8KQ_000561"
     CDS             complement(31669..31980)
                     /gene="rhaM"
                     /locus_tag="ACG8KQ_000561"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9103530.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31982..32827)
                     /gene="rhaD"
                     /locus_tag="ACG8KQ_000562"
     CDS             complement(31982..32827)
                     /gene="rhaD"
                     /locus_tag="ACG8KQ_000562"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9103531.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32841..34097)
                     /gene="rhaA"
                     /locus_tag="ACG8KQ_000563"
     CDS             complement(32841..34097)
                     /gene="rhaA"
                     /locus_tag="ACG8KQ_000563"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9103532.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34094..35554)
                     /gene="rhaB"
                     /locus_tag="ACG8KQ_000564"
     CDS             complement(34094..35554)
                     /gene="rhaB"
                     /locus_tag="ACG8KQ_000564"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9103533.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35707..36576
                     /locus_tag="ACG8KQ_000565"
     CDS             35707..36576
                     /locus_tag="ACG8KQ_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9103534.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
BASE COUNT        10639 a         7703 c         6713 g        11551 t
ORIGIN      
        1 gattaagctt cattattata atataaagtt taaatttggt ttattactta ctttttctta
       61 ttctctagta agctaatatt gaaaaaaatg taatcagatc attattttta tatgttgtaa
      121 cttcctgata agaaaactaa ttaaaagaaa gaggtaacta cataaactaa ttaaaaaagc
      181 taacttcata gttggaggat aaatggaaga aacttcaatg attgtttctc cttgtgttcc
      241 ttcaatcgac actagtccta ttatcaatct ctcttttttg tccatttact tttgcgactg
      301 ttccccaata atcaaataca ggaagcgtta ttttttgtcc tttagattca tgtactgtat
      361 agactatttt cgaatccatc acttgataag gcatagtatg ccattccttg ccaattctta
      421 cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca tagtcataaa
      481 gtcctccggg aaggagtgtc gtgtaacgaa taagattttc ttgattcatt accaagtgat
      541 ctctctgagt gacaacttta tgttcatagt ttgcttttgt tgcagcagat acagccaaca
      601 gtaataaaac aattgccgtt atattaacta cttttctttg cttagctgat gtgctcttca
      661 tgaaaacgaa cgataaaatc aacgagccaa acagtacttg aaagcctaag attcgccaag
      721 gaaattgaat catttgaatg ggtgtatttt gtaataaaga ccatggaaaa aatttggtgg
      781 ataagagcca agagactacc gcactaaggt aaatcacttt ttctttgcga ttaaatcctt
      841 taaaaaagaa gagtggacag atcaaagaaa taataccaat gattcctacc gtaaaggttg
      901 tcaggtcatt attcatcgca ccaatcaaac tatccgagac atttaatgct tggtgttgta
      961 aggtcgtttt atctggctca ttaatcgatt gaaccatgat ttgtcggaac atcggatacc
     1021 aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt tgacaactcc acaatcaacc
     1081 ggatgctttc tttttttctt gtttcactta ctacaaagcc gcctaagact agtaagccaa
     1141 cggctattac actcataaaa gtggaaagaa catgtgtata aatgagtaag ctcatactga
     1201 ctgctaatgg aattgcacat ggctttttct cataaatcag tgcgtaagca taatacataa
     1261 ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc agaacgatga taaatatcaa
     1321 tcgtacggta cccataaaat gtatataaac agctaaatat aaaagccgca tcgattcttt
     1381 ttaaaaattt tagtccataa tgaaacatca gcatgatagt acaaacattc aatagccaaa
     1441 catataagat ataactaacg attaaattat tacttatcca ataaaaaatg actgccggaa
     1501 aaaaggtcaa gtagggataa aaataattca ctccgctccc ataatgatta aacgtcgtaa
     1561 aattgattgg accagaaaaa attgaagata accctttgac tctagcaaaa tggaaggcaa
     1621 tatcccccga tgtagaaata gtatgaacat ttctaaaata attgattgaa taaagtccag
     1681 caataattgc taggagaatg gacaatatta tttttttctt tacttgcatt acttaattat
     1741 atactccttt catggattgc tttatctagt ataacttaaa ttttaaataa atcgttattt
     1801 atttttactc tcacttaaaa aagcactaaa attataagag agggaggtaa tggtctgaac
     1861 accaagaatt gttccaacat cgaattgctt aactaatgag ttcaaagctt aattatgtgg
     1921 ctcttatatt taccgagcgt ggattgcgat ctctttagcg aaacaaacat ctttcataaa
     1981 aaaaacagcc atatccgctt tcttggatat gactatttac tactacttta ctatctttca
     2041 ttcttcttgt ctcacttatg ataaggcgcc cccgcattga tccggaaggc acggtagatt
     2101 tgctcgacaa ggatcagacg catcaattga tgtggatacg tcatttttcc aaaagagatc
     2161 tgagcattgc ttcttttcat gacggcttcg ctgagtccta aagagccgcc gataacaaaa
     2221 acaaggtggc tttttccttg gatacctaat tggtcgattt gtttagcaaa agattcactt
     2281 gtaggatttt tcccttcaat tgctaaggca tagacgtatt cttgttcttt gattttcgct
     2341 aagatccgtt ccccttcttt ttcttttact tgtctcattt gcgcttcgct taaattctcc
     2401 ggggcttttt cgtcaggaac ttcgatcaat tcgatttttg cataggcatt cagccgtttg
     2461 acatactcat tgataccttg gatcaggtat ttttctttta attttcctac tgagataatt
     2521 ttgatattca tgttttctct cctttggtct tcatgctcac tttgtttatt ataatcaatt
     2581 ttttcgtcta tatgtttatt ttggctattt ttgttaaaaa gatttcccca acagttatgc
     2641 acaacatcca caggtttatc cacagaaaac agtgttattt taccttattt catcacattc
     2701 attaggcaaa catacgtttt ataagaattt atccacttta tacacaggct gtggatatgt
     2761 tttatccctt atttttccaa tcctatccgc gtagttttac acagtatctg tggaaaaaga
     2821 aaatcaaata ttttcatctg aaaacaatga tttttcctaa agtttttcat aaaaagttca
     2881 cagtctcaaa gtagactaaa gtcaacaaat aagactatac taacagtaga taaatgaatg
     2941 taattggagg aggaacacat ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa
     3001 aataatagcc tctggcgtaa gttaggtctg gggcttgttg gagggatcgt tggaggacta
     3061 ctgacagctg gcatttttta tgcagcgatg ggcactggca acaatagttc tactgctaca
     3121 agcggcaatc agaattctgc cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat
     3181 tctgatatca ccaaagcggt agataaagtt caagacgctg tcgtttctgt tatcaactta
     3241 caaagtcaaa accaatcaag cggttttggc ggactattcg ggcagcaaga agaaagtagc
     3301 agctcagatg atagtaattt ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa
     3361 gacgggaata ctgcttatgt agtcacgaat aatcacgtag tagatggcca gcaaggtctt
     3421 gaagtcttga tgaaagacgg tacgaaggta aaagctgaat tagttggaac agatgcttat
     3481 tcagatttag cggtgttgaa gatcaattca gacaaagtcg aaacagttgc ctcttttggt
     3541 gactcaagtg ctctgaaggt tggtgagcct gcgattgcga ttggttcccc actaggatct
     3601 gaatatgcaa attctgtcac ttcaggaatc atttcttcct tgaaccgaca agtgacaagt
     3661 acgaatgaat cgaacgaaac agtcaatatc aatgcgatcc aaacagatgc agcaatcaac
     3721 cctggaaatt ctggtggtcc tttagttaat atcgaaggac aagttatcgg tatcaactca
     3781 agtaagatcg caagtacttc tgaatctact tctaatgtaa gcgtagaagg aatgggattt
     3841 gctattccaa gtaacgatgt agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg
     3901 acacgtccag ctctaggtat cacaatggtc gacttatctg ccgtttctac tcagcaacaa
     3961 gaacaaatct tgaagattcc ttcatctgta actaacggtg tcatcgtgac aaatgtccaa
     4021 acagctacac cggctgaaaa agctggattg aaacaatacg acgtcattac taagatcgat
     4081 gacaaagaag tttcttctgg tgtagaactt caatctgctt tgtataagaa aaaagtggga
     4141 gattctgtca aaatcactta ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg
     4201 acgatcgatc aatcggcatt aaaacaaaat caaaatagtg aaaattaata aaaaagaggt
     4261 tgtgacaaag acttgtcaca acctctttat tttggaaatg cttctccgac tacggcatcc
     4321 tcaggcaata ctaagattcc tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg
     4381 gaacagatca ttccatagct atctacccct cgcaaagttc ctggccagat catcattcca
     4441 tctggcatca tagctcctgg ttttgcaacg acaacttttt gtccagcttc gatattgggt
     4501 gcaccgcaaa cgatttgcaa tactgtacca ttgtctactt ctgtctcagt aatcgataaa
     4561 tggtcagaat caggatgtgg gacgcatgtt tttacgtacc cgacaacgat cttcggttca
     4621 tgatcagctt caagctgttc ttcgaatcct gcttctttga tcaatcgatt caatttcgct
     4681 aattcctcat ctgttaagct tacttggccg ttcccttcga ttgttagatg atcggagata
     4741 tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc aacattccat
     4801 ttgcgttcaa cagtgttttc tgttccttta tcgtcggcta taataaccat caaggtgtct
     4861 ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc ctttgctgag
     4921 atttatctta acaaaaaatt gcattcactg catgaaattt ttttcatgct ttatttccga
     4981 agaagcctct tctggaacac catttattcg taacaaggga ctgtgacaag acttttgtca
     5041 cagtcccttt actgttttag ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa
     5101 ttacttggct gttcttcttc gtcagaaacg atcaagacat cacaaggtga atgacgtatc
     5161 acgtagctgg ctacacttcc tgtcataaag cgttcgactg catttagacc tgactgccca
     5221 accatgatta gatcgatctc atattttttt ggcaatttga ctgccatgga ttcacgaaca
     5281 gaaccatatg caataattcc ttctatattt gtgtaatcaa cagatttccc atattcttta
     5341 cattcttcga tcatttcctt ggctgtttca gtttgctgat cgactagatt ttgatcaata
     5401 ggtgcatagc ccataaagtt ataaaattgc tgatcgatta cgtttgctac atatactttc
     5461 ccattattac ggcgagccac ttctaccgct tttttgaaag cagcaagtgc ttgttcacta
     5521 ccgtcgatac ctactaaaat attcttatat tcttgattca tcttgacccc tcctaacaga
     5581 tgtcagcctt ttgttacact ttctaggaac gattcgattt cctgttttgt tttacgatct
     5641 ttattcacta gacgacctaa ttcttttccg tcttggatca cgacaaaact tgggatacca
     5701 aaaatgttcc attcagcggc tacatctatg tacttgtcac ggtctacttc aataaattgc
     5761 caatcagaaa agtcattttc aatttcaggc atttgcggtt tgataaaacg gcagtctccg
     5821 caccagccgg cagtaaagaa aaatacactc tttccagttt ctacgtagcc tgctaattct
     5881 tctaaactat ttgggataat cattctttgt cctctacttt ctgtttatgt atttttcaat
     5941 taaattatac acctttcttt cgaactaaca aacattgatc ctctttttat aaaaaaaatc
     6001 tggcaaaaag caatcttttg ccagatttta tagtttcact atcgcattat tttccgtata
     6061 cgatcgtatt tactgttgat ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg
     6121 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     6181 cgccaatcac tgtacttggg attccttcgt tttgtgtatg agcagcacct gcatctgtac
     6241 cgccttttga aacaaagtat tgataaggga tattgtttgt ttcagcgata tccagcaagt
     6301 actcgcgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     6361 ctcctaaatg tccgaatgtg ccatttttcg tcactgtatc atctgctgca gagcagtcta
     6421 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     6481 cttcttcctg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgcaacg
     6541 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccatgat ttactgataa
     6601 tattctttcc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa
     6661 ggacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatcgg
     6721 ttacttctac tgatttttgt ccgcttgttc ctcgcaataa atgtggggga acggaagaag
     6781 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa
     6841 cgtaaggatt ccagccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     6901 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacggggt gcatccgctt
     6961 cttcatgatg gcgcagacca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     7021 gcggttccat atgactttcc atataagcgc gaacatcttg ttcaaaaccg cttgttcctt
     7081 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     7141 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     7201 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     7261 aaaatatgag agtgtggtaa aataagcaca aggagtaaca agtgagaaga aggaggtatg
     7321 tccacatgta tgaagaaagc gaactaagct attttaaagg cgggttagca gttggaatcg
     7381 gtctaggatt attgagcggg gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt
     7441 cagcagacca agtacttgaa aatatcaagt catcttttct aaaagaaggt gcaatcgaag
     7501 gttcttggat ctcttttgaa aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt
     7561 atcggggagg tctttctcga atagaagatg gcgaaactgt ttattatgaa tttttagcag
     7621 atgctcatac aggaaccgta ttagaaattt caagaaaaaa aggtgcaaca gattaatacg
     7681 tcaaaaaagt gagagaacga atacgcaccc actattcaaa agagaggtat catgtccttc
     7741 ttttgtagtg ggtggttttt tagatagagc tttccaaaca tatattccag tattttcttt
     7801 ttaaataaat gatatttagc gggaaaaact agcatttcct ttgattatga ggtattttta
     7861 atttagcttt tgttttctat agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg
     7921 ctgttctgat tccttgttac aatgaggaag caacaatttc aacagtcatt gcagacttta
     7981 aacgagaatt accagaagca gatatttatg tctatgataa taattcgacc gatcgaacat
     8041 acgaattggc cgttcaaggt ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa
     8101 atgtgatccg ccaaatgttc tttgatatcg atgccgacta ttatttaatg gtagatggcg
     8161 atgatactta tcctgcagaa gctgtacatg gtttgctcga gaaattacgt tctggtgaag
     8221 cagatatggt cattggtgat cgcctatcaa atggaaccta ctttgatgag aataagagac
     8281 ctttccacga ttttggaaat aaccttgtta gaaatacaat tactagaatg tataaaacaa
     8341 aaattcttga tgtcatgacc ggctatcgag gattcaatcg tatctttgtc aaaagttttc
     8401 ctattatgag ttctggtttt caaatcgaaa cggaattgac gatccatgct ttagataaaa
     8461 aattcaagtt cgttgaaatc ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa
     8521 aattgaatac attctctgac ggattcaaag tgatcatgat gattattaaa atgtttaaag
     8581 attataagcc tttgcttttc tttgggattt ggaccatctt cttcttttta tttggattgg
     8641 tcacggggat tcctgtgatt cgcgaatata tgctgacaag ctttattaca cgcatccctt
     8701 ctgcgatttt aagtactgga ttgatgactt tagcacttct ttcattagta acagggttga
     8761 ttttggatac agtcgtcacg aacgctaaga aagaatatga attgaatcta tatcatgttt
     8821 atcatgaata ccacagtaga aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat
     8881 tcttgatcct ctttttaatt aattatttcg ccaaacgata gatggcatct gcatagattg
     8941 ctgttgctct gaacaagtca tcaagactca taaattcgtt tgcttgatgc attgtatcag
     9001 tatatcctgg gaacatcgct ccataagcaa cgccacgttt caacaaacgt ccgtaagttc
     9061 cgccaccgat gatttgttcg tagccttttt ctccagtgtg gtcttcatag acttgcaata
     9121 atgtagccac caatggatca tccattggta cgtaatgtgg ttccattacg cgagcacctc
     9181 gagtaactgt tgcgccttct tgccccactg tttgttcgat gtcactttgc aatccgtcta
     9241 ctgttacacc ttttggaaaa cggaaattca tattgatgaa gttcgcttcc tctgtaccat
     9301 tttcttcaaa agcaaatagt ccagcattca ttgtcaattt gcccattttt tcatcttcat
     9361 aagcgactcc tagtttttct ccataaaaat cttcatggac ataagcagct gctacatgga
     9421 taaattgttt agcagatcct aggaattcat agttatctaa gaacaatgcc aagaaagaac
     9481 ctgcattgat accagattgt gggcttgcac catgtgcgcc tttaccaact aattcgattt
     9541 ttacatacgt attgtccgct tcaatcgttc cgcttactgg atttgcttcg atgaattggt
     9601 aaaatgcttc ttccatcgcg atagctgcat ctgcacttgg tacttgaagt gctgcagttg
     9661 ctgttcctgg caccatattt tcgcgaagtc cggaaacaaa agatttcaag acataatctc
     9721 ctgcattgtc gcctttgaag cgtaaagcta aagaaacatt tcctttttca ccattgatga
     9781 ttgggaattc agcatctggt gagaaaccaa aatctggttc ttcttcatgt tcgaaataat
     9841 aagccatatc gccccagccg ctttcttcat cgctgccgac tacgaagcga actttttttg
     9901 atagtttcaa gtcaagttct ttaatgatct tcatcgcata gtaagctgcc atacttggac
     9961 ctttgtcatc gcttgatccg cgtgcaaaga tctttccatc cttgattact ggttcataag
    10021 gatctgtgtc ccagccatct cctgcaggaa cgacatccat gtgtccaaat atccctagcg
    10081 tttcgtctcc ttcgccaaga tcaatatgcc ctgcatagtt atcgacattt tttactttaa
    10141 atccatcacg ttcgccataa gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa
    10201 atggtgcatc tggtgttact tttgagtcat cgcgctcgct gttgacacgt aaaagatttt
    10261 tcaagtcttc aagcaagtct tctttacgtg cttctacttc tttttgccaa tcaattgtca
    10321 taaagttaat cctccactta tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa
    10381 aaatagttca aaaaatctga caattccttt tcttatctgt ttttctctaa aaaaaatata
    10441 cgctataatg agtaaaagga ggtttttaga tgctgataaa cgaacaacta gtgaaaagag
    10501 tcatcacacc tcgtccaaaa atttcacata aaggaacatt tggacgtatc gttttgattg
    10561 gcggcaatga acaatatggc ggagcaatca ttatgagtgc tttagctgcc gtacacagcg
    10621 gagcgggtct cacgactgta gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc
    10681 tgccagaagc aatgattgta gtttggacgg atactgacca gtttctttcc gtaataaagc
    10741 aagcagatgt actcttgatt ggtcccggat tgggtacttc ttctaagagt ttggaacgtt
    10801 tcaaacaagt actagaaaac caatcaaaag aacaatggct ggtgattgat ggctctgctt
    10861 taagtttact tgctgctaat catcttttgc taccctatcc cgaacaaact gtacttacac
    10921 ctcatcaaat ggagtggcaa cggttgagtt ctctaccgat acaagaacaa cgggataaag
    10981 aaaacaaaaa gaagcaacag gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa
    11041 aaatttatgg tcaccagcac ttctacacca atcctttagg ttctgctgct atggcaacgg
    11101 gcggcatggg tgacacttta gctgggatga tcacaggatt tttagcccag tttcctcaaa
    11161 atgaccaaac gattgcagca gctatttatc ttcatagttt cattggagac cagctggcaa
    11221 aagaacaata tattgttttg ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt
    11281 atttttgtaa aaaatcaatg gaaaacgagg catgagtcat ggagaaattt acaggttatg
    11341 tgcgagaacc tttttattat gaaacagatc aaatggggat tattcatcac agcaattata
    11401 tccgctggtt tgaagaggct cgggtagcct tattggtaca tctagggttt ccctataaaa
    11461 aaatcgaaga atctgggatc atcatccccg ttctggaagt aaattgcagc tataaagaaa
    11521 tgatccatta cggagaaaaa gtgagcatcc atccaactat ccaaaagtat acggggacac
    11581 ggttggattt tagctacgaa attgtgggaa ctgaagacaa aaaattaaaa actactggca
    11641 gtagtaaaca ctgtttttta ctttctgatt cgcaacgatt agtgaagctg agcaaagtca
    11701 atccggagtt agatcagtta ttccaagcgt acgcttcttt ggatgatgta aattcttaat
    11761 aaagaccgtc taagcatttt tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa
    11821 aagcaggtga tccccatgta tgtcgttaaa gtaatgcacg gttacatcga taaaactggc
    11881 tgtcgcactc gggaaaaaaa cttagacaat ctgctcattt tcaaagacaa aaaagaatcc
    11941 gaagcttttg ctaaaagaat cggcggtcgt gtcaaaccga tccaagaggt acgaccagat
    12001 tgattcaata tttttctttt cgaggctcta ataggcatgc ctatcagggc tttttttgca
    12061 gactgtgttt ttatgcccac acgatttgta agcgatttat gttataatag attatatctg
    12121 catagaaagg aaggttaaat ggttatcaat aatttgcaac agcaagctta tgaatctata
    12181 cgtaaacaaa tcatttattc cgagttagca cctggaagta aaatctccga taaagtgtta
    12241 gaagaacaac tagctatcgg gcgtacaccg attcaagaag cactgatcca actgcgcaat
    12301 caagagcttg taaaaaccat tccacagtcc ggcacttatg tttcaaaaat cgatatccga
    12361 tctgcttcat tagcccgtta tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc
    12421 agtgcgaaaa tgaccaagca agatcaagct aaactagaaa aaatactaga acaaaccgat
    12481 caggcagtaa tcgctcaaaa taaaaaagct ttttttgatt tggatactga ttttcaccga
    12541 acttgttatg aaattgctgg aaaacgagaa atttgggatt ggctagaaag ttatagtacg
    12601 cacttgaacc gtttcagatg gttacgtttg acgatttcag aattagactg gggtcgagta
    12661 ttagatgaac atcagacaat gcttcaatca atgattgatc ataactttga tgaagtcgga
    12721 tttctttgca cgatgcattt acatatgatt atcgaggagc aagaatacgt gattcataat
    12781 taccccgatt attttgaaga tattcaggac agacccatca aataagaggt tttggaatca
    12841 gcgttttgac ctgagtatta gagaaagaaa cggcaaaata gttcctcata ttttgccgtt
    12901 ttttttgagc taataccgag ggtcagctga ttgcacaccg tttattgaga ggttgtgaaa
    12961 aagctgccct gcatcaagta ataagaacag ccaagtaaaa atagcttctt atgtttttaa
    13021 cttggctgga cttattaccg cagatgcaag cttttgaaca ccgttttcca agaggtcgtg
    13081 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    13141 ttgaatattt tgacttgtga ccgaggagtt gacaggagaa catcgtttat tcggaaaaaa
    13201 aggactgtga cacgactttt gtcacagtct ctttatttgt aatctaaatc atactccgta
    13261 catctttttt ctattttttt tatttcttct aagatccctt ggctacgaat ttcattatat
    13321 ggccaaagta agactgcctc taatacataa ggcgaaaaat cgctttgatt catctctttt
    13381 gaaaaactca tcgctgctat ctcgctcaat gcacgaatgt ttgattgatg tgtataaaaa
    13441 ggcaaccgcc acagcacaat tttttcgctt tgcgtataaa ttcccttaac agaagcttcg
    13501 atgtggtgaa atagttcatg tccaataatc aaagcttgcg ctgtttcttt agagatcgaa
    13561 gctagtcctg aacattttaa tttttctaac ggctccgtca tcaatcgaat cccttcatct
    13621 tctgcataag ttgcaaaagt gattcgttcg tttatttgct ctgattcaca ggaaacgatt
    13681 ggaacagcca tttgttcaca aatttctcct acatcggcaa aagggtactt ttttttgatg
    13741 ctacatgccg tttcgatacc acaatcgatt gattgttgta taagaatttt ctgcttggac
    13801 aaaggaatct ttttccaaaa taaatctcgc cgaaactggt agaatcccca aatttcttcg
    13861 gggatctgtt gtaattcgtt tagtaactct tctaacttat gtttgtttga tatttccggc
    13921 aacgacgatc acttcttcct ccggttccat agtagatact tccagatcca tcaaaagctg
    13981 tagttgttcc agatccgtcg ctgtaaaatc agagactctt gctcctaagc agagataaaa
    14041 ttctggtcgg gcaatcaatt cagtttgata aactgccata tcttcccaaa gttgttcgac
    14101 agctgtcata taattggctg cagttgtttt catacatgaa gcatgtccac gttggacctt
    14161 gataaatcct ttttgatcga tgatccgaag acttccagca ttttttgaac gattctgttc
    14221 gccgcataca taaaagaaag gtgtattttc caataagcaa acttcatttt tatttaatcg
    14281 catatcctct gcagcaagct ctagtgcttc ttctgttgtg atctccttgg ttagatcggt
    14341 tgctttcact tccgtagaac cagtagcgat agccgttact tttgaagtct gtggatcgat
    14401 ttctacgtgg acttcgatcg attctggtgt agcgcctgat tcaatcgctt tattcatcgc
    14461 ttcatttttc aacgatcgta tatcttcttt acttggcgaa gggatgatac gttctacaac
    14521 atctcggacc attgcaagtg ctacaccaat agaagaaatc acttcggcgt tttcaggaat
    14581 cgaatatttc acacccattt tattactaaa gtaagtaatc aatgaggcag cgccgccccc
    14641 tacacctaca agagaaatct gatctttctc caagtgatac ttgtctgcca attctaaaat
    14701 caccggttca atcttagcat aggatttttc catgatctgt tctgcgatgt cttcaaccgt
    14761 cgtatgacaa taatcagcta atgcttggat ggcttttcgt gcagatggga catttccata
    14821 agaaaaatgt tcttcttgca ctaatccaag gacattggct gcacatgtat ttgtaatagt
    14881 cacttcttct ccgtcttcca tcactacctt gacgtaatca gcagggtctc cttctttagg
    14941 agagaaaaat tcaaccttag gacctttgat tttttctgtc tccgtaaata ctgcataatc
    15001 tagaccggca atatgtgcag aacgtggacc gacatcgatg attcctgact gattagcccg
    15061 taccatcgaa ccgcctgcca cacctagaac ccgaacatct aatgaagaga tatatgttgg
    15121 gtggccacct acaattgaat aatcgattgc tggacgtcca tttttaatga ccccgatatt
    15181 ggttgttgtt ccgcctactt caaaataaac accattagac gctctcaagt acatcaaaga
    15241 gcccatgact gatgctgctg gaccggaaag catggttaaa acaggtcgtt ttttcatttc
    15301 attgatttcc attactccgc cgtctccgcg cataatcatc aaagaaacat tgacccctgc
    15361 ttctcgtacg gaatcttctg tagaggttgc cgtatctagc atttttggta aaatagatgc
    15421 attgattgct gctgtccgag ttcttcttgt caatccatat aattttgtga tgtcagaagc
    15481 catcgttgta gggatcgatt ttaccgatgc tgcttcataa acaactcgtt ctggttcccc
    15541 attatctact ccaaatgcca tagaagaaac caagacctcc gctctttctc tttctaaaga
    15601 agaaatcgtt tctgaaacac ggtctacatt taagtgtttg acaggtaaga atgcattgac
    15661 aatctctatt tttttcttat tccctaaatc gatatcgttc aatctagttt gtcgttttgc
    15721 taaaaaacct tctaatccac cttttgccat cccgattacg ccgacttttg ctacgtctcc
    15781 ttcaatcagt gcattagtcg cttgtgtggt agaatgtgca acaaacacaa catcttctgg
    15841 actgatatta ttttccagca aacaattttg aaaagactga acgacacctg cggctactcc
    15901 acggacatca tcgtgggttg ttttcactga agattttccg atgatttcgt gcgtcgcatt
    15961 atcgattgcg actgctttcg tatgtgtacc gccaacatca attcccattc gcacaaaacg
    16021 tttcattttc ttttcctcca ttcatctaat cgtttttatc gacgttttgt ttactaggcg
    16081 ttcccgtaca ataagaacgt gatcaggcat aagatcgctc cgatgatcca accagttggg
    16141 atggatttct tcatatattc tcgagaagtt acttttgtat aacctaatcc ccaagcaacc
    16201 caagattgtg taatatctaa atgctgagga gcaatcgttg tgattgcaaa taacgggaac
    16261 aaaaaggctg gcgacatcgt tggattaaca gctaatacga cagctaaaat agctgagcct
    16321 gaaccgacta gattcattgg accgcggaaa aatcctagtg gtgtcaatat agcaaatacg
    16381 attgccaaga ctaatggtga ttgcggtatc caatctccaa aaatagctga aaaataagga
    16441 gaagcatacg cagctgaatt attgaacatc gctaaggtca tcaagaaacc aaccattggc
    16501 gcaacatcta ctgcgccatc tgtaaataat ttcgcaaact gacgacagat ttctttataa
    16561 cctccgcgca attttccagt aaacagcaat gcccagatac ctgctagaat aaaacctaag
    16621 atgattggaa gatctaaaag aacgacccct aaaactggta ataatacggc taaccatgag
    16681 atcattggtg cgtcgccacc ttcagctggt acgtttgctg ccattgcata gcgtttcttt
    16741 ttgttcattg atatattagc gaccgttagt accacaacta agcttacaat catcccaatc
    16801 ataccaattt ggaaatagtc attataagta tagctttcag ccgctggatt gaaaccagca
    16861 taaatcgttt gatattgttt gaaattgacg atattagcaa agattccagc cataatcgag
    16921 cccataaatg agaacagtgc aaccggtact gaaataccta atgacatcaa gattggcaac
    16981 acaataactg caatagaaat cactggtccg attcctgtca tcgacatgaa tataacagcg
    17041 gttacaatac atagtaagct catcgtgatt cttggcttgt cccctccaag ttcaactacc
    17101 tttcgaatca gggtagctgc gattccgcta tctactagaa cacgcccgaa aaaggcacca
    17161 aagaagacat ttaccaagat cgatttggcg tactcagctg gccccgtcgt atagatatgc
    17221 gtcaacacat caattactcc ttgcccttcc atcgccgggt ttggtgaaaa cgtgtttccg
    17281 actagcgcta atcccatcca tacagtagcc atgatggcaa agccgaccat caagttgtag
    17341 ccttttacac agtaccagac catgaaaaag aatgagataa ctaacaaaat accaataata
    17401 atatcactca tgttattcct ccgtccattt tgtttttctg ataagcaaac taatatatca
    17461 gtcaaactca ttctaactta tgtagttagc gctttcatta aagggtgacc ataaatgttc
    17521 agttttcaat taaaaaacct taattatgct attttagact tatacgttat tgtcctgttt
    17581 catttgatgg aggattgtaa ataaaaaaag aggaaagaac ataacttttc gttatcttct
    17641 ttcctcttta taattgttta gcttatttag ccaaagcttc ttttgcttgg tcagccaatg
    17701 cagtaaatgc tgctgcatcg ttaacagcca agtcagctaa cattttacgg ttgatgtcga
    17761 tttcagcaac tttcaaaccg tgcattaatt ttgagtagct caagccattc atacgagctg
    17821 ctgcgttgat acgagcaatc cacaatttac ggaagtcgcg tttcttttga cgacgatcgc
    17881 gatatgcgta gttgtatgaa ttcattactt gttcttttgc tgttttaaat aaagtgtgtt
    17941 ttgaaccgta gtaacctttt gctaatttta gcactctttt acgacgttta cgagttactg
    18001 ttccaccttt aacacgtgcc atgtataatt cctcctaata aaaataaatc ttcagattct
    18061 gttatttgta cttcttattt catttttgct agttgttggc gaatacgttt gaagtcgcca
    18121 ctagaaacca tgcttgcttt acgcaattga cggcgttgtt tttttgtttt gccgtggaaa
    18181 cggtgacttg taaacgcgcg gaagcgtttc aatccgcctt taccagtacg tttgaaacgt
    18241 tttgctgatc cgcggtgtgt tttttgtttt ggcatgacta tatttcctcc tcaaaatctt
    18301 tcgttatccg actatcggct gtccaacagc cagttagtca ttcaaattac ttactgtcgt
    18361 ttttcggtgc cagcgtcaag aacatgctgc gtccgtccat tttcgctttt tgttccactg
    18421 ttgcaatatc cgcagtttct tcagctaagc gattcaagac tttttgacca atttctttat
    18481 gggtaatggc acggcctttg aaacggatag aagctttcac tttatcgccc ttttccaaga
    18541 acttacgtgc attacgaagt tttgtattga agtcattcaa gtcgatcgta ggacttaagc
    18601 gaacttcttt tacattgatc actttttgtt ttttacgcgc ttcgcggtct ttcttttgtt
    18661 gttcgaaacg gtatttaccg tagtccatga ttcgcgcaac tggtggtttc gcaccagggg
    18721 caactaacac aagatccaag tttgcttgtt ctgcaatatt caatgcttct gcttttgtct
    18781 taactcctaa ttgctcacca tctgaaccga tcaatcgtaa ctctcgtgca cgaatgccgt
    18841 cgttaaccat catatccttt gctatggtca ttcacctcca aatattatga gagaaagaaa
    18901 agcttaccga ataaacggca ataaaaaaac gagttctctt acagaacccg cgcgtacttc
    18961 acctaataga accagtgaaa actatctagc ccagtgacat tatcaactaa ggcgagaagc
    19021 gggtgcttct gcttttattt ctcaaccatc atagtatagc aaaaactaat ctttttgtca
    19081 atgtttttca tacagtttgt cttccttttc tgaaaatgat tagattagca gactttcttg
    19141 cagtgattcc cgcttctttg ctatactaaa gaaaaacgag gtgaacccac atgaaaaaaa
    19201 tcgaacctat tccaccttct cttcctcttc ttacaaaggg agagttttat ttagaagatg
    19261 aggagatctt ctctggtctt ctgatcaacg ggggtgacca aagctatctc gatgccaaaa
    19321 atatggtatt aagagagagt catatccaaa aacttacgat gcaaaaaaca gttttacgtc
    19381 attttgaatg cagtaatgtc atttttgaaa aatgtgattt ttccaatctg gagtggattg
    19441 gtggcagttt ccaccaagtc gtgtttcatc aatgtaaatt aacaggtacg aattttgccg
    19501 aaagttatct tcgagactgt actttcactg attgtattgc ttcgatggct tcctttagta
    19561 gtacaaactt gaaagctgtc tccttcgatc attgtcaatt agaagacagc gaattttatg
    19621 aggtcacttg gaaaaacctt tttttgtctg acaatcagtt gaccggttcg aactggtttc
    19681 gaacttctct gaagggatta gactttacga ctaatttctt cacagcaatc gctttatctc
    19741 aagattcttt ggcgggacta atcgtcaacc aagagcaagc acttgtgatc gcccaagccc
    19801 ttggtttgca gatcaaagac tagatcaacg aatccgccgc cacagtgcaa taagcatcca
    19861 aatagataag accaaactga tgatcaaggc gattccccat ccgaaaacgg aatgggtaaa
    19921 gggtaactgg agattcattc cgaagaatcc tgtcacaata gttggaacgg tcaagatcag
    19981 cgaccagacc gtcaaaaatt tcatcgtgtc attcaagttg ttattcagca aattattata
    20041 agtgccagag agctggtcta aaatctgcga agctaagttc gtcatttcaa ctgcttgtcg
    20101 tgcttcgatc agcgcatcgt ccaactcttc tttttccttt tctgacaact tgcgataaat
    20161 agccaatgcc ttgatttgtt ctaaaagtac tacattttgt tttgttcctg taactaagta
    20221 aaccaaaccg atctctaaat ctgaaagttg cagcagattc ttgttcgttg ttttttctct
    20281 taatttcaga ttaagccgct gacgttcact attcacttcc tcgattaaag gaaaaaagga
    20341 atcagaaatc aaaaacaatg tacgaaacaa taaactgtat acactcatgt ctggcattcg
    20401 ttccaaaagc gatcgaatta gtcgaatcac gtactcggta ttcggcgtgg taaatgtaaa
    20461 caaaccgtct tcttttaaaa taaaagcgat aggactagtt tcataatgat tatcgacttt
    20521 ttgctggtgc ggaacatttg atacaagcag taacgcttca tcaaaagaat cgtactctac
    20581 gcgcgcccgt tcatttttat ctagagaata agtcagcatc tcgtcactaa tgccatattc
    20641 ttcttttaag tttttaacag ctagcacatc gttttgctgt acattgatcc aaaaattacg
    20701 gtcttgatta aaatagtgtt tttcccgcat actctatcct cctgacattt gtttccagta
    20761 taaagaaaaa agtcgctcaa ataaaggttt accctttatt tgagcaactg taaatcaaat
    20821 ggctactttg gtttggcgaa tctctgctaa tttttcttcg atcattttca agacttcttc
    20881 ttttgcttga ggatcttcca caaaattata gcggtctcca tcgatttgga tttttggact
    20941 ttctttgtat tcttcatacc attgatcgta acgtctgttc aattctttgt aataatcgta
    21001 tagacttgga tcaaaggaca attgttcata cgaacgacca cgtttttcga tacgctccaa
    21061 catcgtatca aaagatacac gaatatgaac taataaatcc ggatgttttt tatgggcagc
    21121 atagggaagt tcttccatca tgttttccag cagttcatca tacacacgaa cttctgtttc
    21181 tgttgctctt cctagatctg catttagatg gaaaagcagt gaatcttcat aaatcgaacg
    21241 gtccaataca ttattgtcat ctttcattgc ttgtttgatg cttgcaaaac gtttattcaa
    21301 aaaataaatt tgcagcaaaa aagcatactg ttcagggttt gcataaaata agggcaagac
    21361 ttcattatca tcaatcgatt cataaaatgc ctgactatca aagtgttcag ccatcatttc
    21421 tgttaaactt gattttccgg caccaattgt acctgctaaa acgatcacgc tcatttacct
    21481 ccagctcact tcatcactaa atattgtgtt taactccaac ctttttaaat atacccccaa
    21541 tatctagtag tgtcaattgt atttttttct agtcaaagaa gaaaaaaagt caaaaaattc
    21601 ttaagaaaaa agtgtttttt ctttagaaaa cgtattctta gttttacttc tgagatggtt
    21661 ttagtaaaat aaaggaggaa tgttttttga gacccttagt ggacaaaaaa attcgtgaag
    21721 cagcgcaact cgttttgggc gctttatttt ttgcttaaaa atcactttta cgtgagaagg
    21781 agttagatta tggcaaacta tggccaagga gaatcaagcg gaaagatcat attgatgggc
    21841 gagcacgcgg ttgtttatgg agaaccagcg attgcctttc ctttctatgc aacaaaagtc
    21901 accgcattcc ttgaagagct ggatgcaatg gacgatcaac tggtttcttc ctactattca
    21961 ggaaatttag ccgaagctcc tcatgcatta aaaaatatca aaaaattatt cattcactta
    22021 aaaaaacagc atgacatcca aaaaaacttg caactgacca ttgaaagcac gattcctgct
    22081 gaacgtggaa tgggatcaag cgctgcagtc gccacagcag tcactcgtgc tttttatgat
    22141 tacttagcat ttcctttgtc tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa
    22201 atcgcccatg gtaatcctag tggaatcgat gcagccgcta ctagcagctt gcagccgatt
    22261 tattttacaa aagggcatcc tttcgactac ttttctttga acatcgatgc ttttttgatt
    22321 gtcgctgata caggaatcaa aggacaaaca agagaagccg tcaaagatgt cgctcacctc
    22381 tttgaaaccc agcctcatga aactggacaa atgatccaaa aattaggata cttgacgaag
    22441 caagcaaaac aagcaatcat tgaaaattca ccagaaacgt tggcacagac aatggatgaa
    22501 tcacaatcac ttctggaaaa gctgacaatc agcaatgatt ttcttaatct attgatccaa
    22561 acagcaaaag ataccggggc attgggtgcc aaattaactg gcggtggacg cggtggctgt
    22621 atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat
    22681 gcgggtgctg ctgaaacttg gatacaagga ttaggagtac atacctatgt ttaaaggcaa
    22741 agcacgcgca tataccaaca ttgctctaat caaatattgg ggtaagaaaa atgaagaact
    22801 catccttcca atgaacaaca gcctttcgtt aacattggat gcattttata cagagacaga
    22861 agtcatcttt tctgatagct atatggtaga tgaattttat ctagatggca ccttgcaaga
    22921 cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag aagccggcct
    22981 ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct accgcagctg gattagcctc
    23041 ctctgccagc gggctagctg ctttagcagg cgcttgcaat actgctctta agcttggatt
    23101 agatgatctc tctctttcaa gatttgctcg acgcgggtct ggttcagctt gccgaagtat
    23161 tttcggtggt ttcgtcgaat gggaaaaagg ccatgacgac ttaagttctt acgctaagcc
    23221 agtcccttcc gattctttcg aagacgattt agcaatggtt ttcgttttga tcaacgacca
    23281 gaaaaaagaa gtgtccagca gaaatgggat gcgtcggaca gtcgaaacat ccagttttta
    23341 tcaaggctgg ttagattccg ttgaagggga tctatatcaa ttgaaacaag caatcaaaac
    23401 aaaagatttc caacttctcg gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac
    23461 gactttagct gcccagcctc cattcactta ctggtctcct gacagcttaa aagcgatgga
    23521 tgcggtgcga caattgagaa agcaaggcat tccatgctac tttacgatgg atgctggccc
    23581 taatgtcaaa gttttagtgg aaaacagcca tttatctgag gtacaagaaa catttaccaa
    23641 attatttagc aaagaacaag taattactgc acatgctggt ccaggaattg cgattattga
    23701 ataaagggag atttttatga ttgaagtatc tgcaccaggc aaactctata ttgccggaga
    23761 atatgcagtt gttgaaacag gccatccagc agttatcgct gcagtcgatc aattcgtgac
    23821 agtcactgta gaatctgcac gaaaagtcgg aagtatccaa tctgcacaat atagtgggat
    23881 gcctgtacgt tggacaagac gcaacggaga attagtttta gatattcggg aaaatccttt
    23941 tcattatatc cttgctgcta ttcgcttgac tgaaaagtat gcacaagaaa aaaacattct
    24001 tttatccttt tatgatctga aagtaacaag tgagttagac agttcaaatg gccggaaata
    24061 tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga atgtttttta
    24121 cgcgttgaat ttatctcagt tggagatttt caagattgca gcactagcca atttagcagt
    24181 tcaagataat ggttcctgcg gcgacatcgc tgctagctgt tatggtggct ggatcgcttt
    24241 ctcaaccttc gaccatcctt ggctccaaga acaagaaact cagcattcta tcagtgagtt
    24301 acttgccctg gattggccag gtctatccat tgagccattg attgctcctg aagatttacg
    24361 tttattgatt ggttggacgg gtagccctgc ctctacttct gatttggtcg atcaagttca
    24421 ccgttcgaga gaagataaaa tggtggctta tcagcttttc ttaaaaaata gtacagaatg
    24481 tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta acgttgatcc aacagatgat
    24541 tcgaaaaaac cgacaattac tgcatgattt atctgcaatc actggggtcg tcatcgaaac
    24601 gcctgctttg aacaaattgt gtaatttagc tgaacagtat gaaggagccg caaaatcttc
    24661 tggtgcaggt gggggcgatt gcggaatcgt aattgttgac cagaaatctg gcattcttcc
    24721 tttaatgagt gcatgggaaa aagcagaaat cactccactg ccgttacatg tctatagcga
    24781 tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc taaagccttt
    24841 catgataaac aaaaaaacga atttgacttc gtacggatca tccataaccc tttgccgcaa
    24901 atagcagttt ctgatgtcga tctaagtaca caggcagttg ggtttacact aagcagccca
    24961 ttttatatca atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt
    25021 gcaatcgtcg caagagaagc agatttgatg attgccactg gttcagtcag cgctgccttg
    25081 aaagtccctt ctttagccga tacttatacg attatgcgac aagaatatcc tcacgggaaa
    25141 atcattgcca acatcggtgc agggacttct gtcgaaagag ccaaagaagc tatccgattg
    25201 tttcacgcag atgccttaca gattcatttg aatgcacctc aagagttggt tatgcctgag
    25261 ggagaccgtg attttaccaa ttggaaagta cttattcaag aaactcaaac agccattgac
    25321 gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa gagaaacgct caatgatcta
    25381 gctgctttag gagttcatac agttgatatc agcggtcgaa gcggaacaag cttcacccag
    25441 atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt tagctgactg ggggcaatcg
    25501 acagtctctt cattgcttga agcaaatgaa gcagacactt ctatggaaat cctagcctcc
    25561 ggaggtatcc gcaatgccta tgacatattt aaagcccttt gtctcggcgc aaatgccgtg
    25621 ggtacttcag gaaccgtact gacgcatctg atgaatcacg gcgtagaaga aactattata
    25681 ctgatgaagc aatggcaaga agagcttcgt ctgctttata ctatggtcgg cgcaacgaat
    25741 accgcggccc tacaccaaca atctctgatc ttttccggac cagtgaagga ttggtgcgaa
    25801 gcaagaggga tcgacttagt gaagtatgga agacgcacag aaaagagcgt gggacaaaag
    25861 taaagaacac ttttgtctca cgctctaaac acgtacaaac ggcggaagta aaagcaactc
    25921 cttcggaaat aagctaaaat tcacaaaaat ttgaaaagca attttcgtga attccttctt
    25981 atttcttgga gttaaatgct tctgtcccag cctctttttt cgaataaatg atgtttaatc
    26041 agctgtcctt cggtattcgt tcaatccgct gatttcacaa cctctccact aaacagtgta
    26101 ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag
    26161 acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa
    26221 tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga
    26281 tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa
    26341 cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc
    26401 gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat
    26461 tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat catatccgga
    26521 ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag
    26581 tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga
    26641 atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca
    26701 tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata
    26761 ccttcatcaa ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct
    26821 aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt
    26881 ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt
    26941 taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc
    27001 ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat
    27061 aatggcttgt tcattgtcta caactaaatg ttttgtttca tctggtttgc ccatcatgtg
    27121 gaacacacgt gtatcttcat tggcataatc aaagtagaca taagcttcca tacgacgttc
    27181 atgtgtatga caaggcatcg tattccaagc gcttcctggt tctaaaatcg tataacccat
    27241 ttgtaattga cagctttcgc atacatttgg atggatgtat tggtagattt tacgttcatt
    27301 cagtgtcagg ccttcaccag tctccattgg ttttacttgg tcgatactga tttttacatt
    27361 tggatattta tgatgagctg gaacagaact aatatagaat ttagctgggt tttcagcatc
    27421 ttgagaagaa aatacaacat gtcgtgtttc ttttccgata tagtatccgt cttgtttttt
    27481 catttcttct ttttgcccat cgatctcgat gaatcctggt ccaccgatat tgatcacacc
    27541 tagttcacga cgctctaaga agtaatcaac acctagttct ttgtctaatg tgatttctaa
    27601 ttctttttca gtaggtgtca ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa
    27661 actgattttc cctggttcaa ataccttttc tactaaaaat tcttttctca attgttccgt
    27721 tgaataatga cggatatctt ctggactgtg tgtataacgt gtttccattt cttgcatttt
    27781 cttctactcc ttctatcgaa tgatttttcc agagccgcat gctaaaaatt gtgcgacttc
    27841 ttcttgacta aattggttgc agtccccatg gatcgtatgc ttcaaggcag aagctgccgt
    27901 agcaaaatca atgatttctt gcggagacat attggataaa atcccatgga gcacgccacc
    27961 tgcaaaagca tctccgccac ctactcgatc cacgattggg gtgattctat ggacttggga
    28021 ttccacatat tcatggttca tccacaatgt ccccttcaat tcattttcac ttgctgaatg
    28081 aacggtacgc ttggtcgaat aaaacacctg aatattcggg aacttttcct gcattttttg
    28141 ataataataa attagaggtt gttcttctgc ttctggcgct ttctcgatac ctaacaaata
    28201 aagagcatcc atttttccag ctgagcagat atctactaaa ggtaaaagct gatggattac
    28261 ttcgctggct tcttgctgtg tccataattt tccacgatag ttgatatcga agctgatcag
    28321 gcagccagct ttttttgcag cttctatcac ctgtttcgtc attttttgcc attttttaga
    28381 caaagctggg gtgattcctg aaacatgaaa tatatctatt ccttggaata actcctctag
    28441 cgaccattcc aaatgatcca tttcagcaaa acttgatccg gcacgatcat agatcactga
    28501 ggcggcgcgt tcgccactac cagtttccat ataataagta cctagtcttg atccaccaga
    28561 taatacctgt ttcgtatcta ctccataaca ttgcaagtgc tttttgactg cttttcccaa
    28621 tgcgttatca ggtacttttg tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat
    28681 tgctacgttt gcttcccctc cgccataatg ggcagcgaat cgttcacttg aatcgatacg
    28741 ggttccctgc tctgtggaca atcgcaacat gatttctccg agtgtcacta cttttcccat
    28801 aagcagctac ctcttgattt ctttcatttt tgctgcatac tgttgcgcga cttccgttac
    28861 tttgtcaaaa tctcctgtag ctgcgggtgc taaaaggttc ccacctactc cgactgttac
    28921 gacaccagca tcgaaccact ctttcatatt gtccagactc acaccgccag ttggcatgat
    28981 attgacttga ggcatcggcg ccttgaaggc agaaatgata cttggtccat aagcgcttcc
    29041 aggaaatagt ttgacgatat ctaccccgct tttcagcgct gttttgatct cggtaatagt
    29101 catacatcct ggtaagtaag gaatttgata cagattgcac atctctgccg tttcttgatc
    29161 aaaggaaggg ctaacaatat actctgcgcc agctaagata gccaagcgag ctgtgacagc
    29221 gtctaatact gtgcctgcgc caatcactat ttctggttgg tctttgtaga atgacaaaag
    29281 ttctttgatt acttgatctg cttgagggac tgtaaaagtc aactcaatac cagttagacc
    29341 acctttgatg attgcgtggc aagcattcaa agcttcttct ttgctttttc cgcgtaccac
    29401 cgcaatcact ccggcgtttt tcaatcgaga taaaatatca atttttttca tcttctccac
    29461 ctatttccta tttagaaatt ttattttata acttggaaat ctatacccat attatacggc
    29521 agaaaagaaa aattgcaaac gcttttttca ttatttttga attctttgct cgatttcgct
    29581 ttttgtttgc ttgagggcac tgattatttg ttcttttgtc tcatctgtta aacgatattt
    29641 cggcatactt acactaaatg caccaatgat ttgttcgttt ttcacgattg aggctcctat
    29701 acaaaaaata tcacgttcga tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt
    29761 tttgatttcc tttttcaacc gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa
    29821 cggatgcttt tcaagataat cagtgtactg ctcttcactg aattctgcta atacggcttt
    29881 gcccatcgca gaattgtata atggtcgggt gattccgatt tttgaagaca tgcggatcgt
    29941 ttgatgtttt ggatctagtt tgttcacgta caagatctca ttgttgtcca atacccctaa
    30001 gtggatagtc tcatcgatct tttgctgcag ctgttctaaa aacggtaaag tcaactctac
    30061 caaatcgatt tgttcaatgt ttttattcgc gtagcgaata agctttgcac ctaaacgata
    30121 gtttttctct gaatttttat ttacgtagcc gatcatcaac aaagtatcta gtattttcaa
    30181 gactgtagaa gaggtcattc ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt
    30241 ttccgctaaa tagtctagta ttttagaggc tttgatcaat acagttccat aaggcttttt
    30301 attttccata taatattctc ctatttcttt ataagaaaaa ttatagcgga aataccatca
    30361 atttaaaaga gatatccaga tataaactac catttctctt tacaggcaat ttaatgttga
    30421 agatccgctt cgtttttaca tcaaactttg atataaagca atgatttctt ctactgtcgt
    30481 ttctcttgga tttcctggtg tgcatacatc tcgcaaagca tcttctgcaa ttgctggaat
    30541 atctgcttct tttacaccta attcagaaat ggtttctggg attccaaccg ctttgctcaa
    30601 ccgatcgatc tcaccacaag ccgcttctct tacatcttct aaagacattt cagctgctcc
    30661 tttaatccct agaacaagag cgatctcacg gtacttttct ccagtatatt ctttgttata
    30721 tttcatgaca gttggcaaaa gtgctgcaca cgctacgcca tgaggaatgt tgtaccatgc
    30781 acttaacggg tgagccatcc cgtgtaccag tcctaaaccg acatttgaga agcccattcc
    30841 cgcaatatac tgtcctaatg ccattttttc tcgcccattt tgatcgccat ctacagaagc
    30901 ttccaaagaa tggccgatga tttcgattgc ttttaaatgc agcatatcag tcatttccca
    30961 agcgcctttt gtgataaacc cttcaatagc atgcgtcata gcatccatac ctgttgatgc
    31021 agccaatttt ttcggcattc ccatcatcat atcgctgtcg atgaacgcga cgattgggat
    31081 atcatgagga tcaacacaaa caaattttcg gtgatttact tcatctgtga tgacgtagtt
    31141 gatcgttact tctgctgctg ttccagaagt cgttggtata gctaatatag gtaaacatgg
    31201 atttttcgta tcagcaacac cttctaaact aatgacgtct gaaaactctg gattcgtcaa
    31261 aataatccca atggcttttg ccgtatcgat tggcgaacct ccaccgattg cgatcaaaca
    31321 gtctgcctct gcctctttaa caaaagccac tccatctttt acattttgga tcgttggatt
    31381 aggtacgatc ccgtcgtata aagcataagc gattgctgct ttatctagaa gttctgttac
    31441 ttttgttgct ataccgtgct cgatcaatcc tttgtcagta atgacagctg ctttgccgaa
    31501 tcctcgctgc tgaaattcag ggacgatcga ctcaatcgct ccttttccaa aatacgatgt
    31561 ttcatttaaa atcattcgat tactcatttt ctattcctcc taatttgtat tatttttcac
    31621 aataaaaacg aaagaacccc aagcaaccgg ttgcccagtt gcttgttgct acagttcaaa
    31681 gactggcatc aattcccaac tgactggaga ggcatctgga tttgtctcca tgatatcttc
    31741 catgtacttc caccattttt gattgataga tgtatttgcc attttttccc ataacttttc
    31801 atcttcgatc actaagtaac caaataacaa acttgtactc ggatcaagat agatcgaata
    31861 gtcgatgacc ccgtgttcac gaagtgctgt acgcatttct ggccaaagtt catcatgtct
    31921 ttttttgtat tcttcttgtt tttctgggta aactttcatg caaaaagctt ttttcaccat
    31981 gtcagacacc tgctttcgta tgaacttcct ccaaataacc tgcttttgga gtgacaccaa
    32041 acgcatctgc taaatgccac aggttttcgt ccgtgattgt ttgcaagatt ggtccttgcg
    32101 ctttgacgta cgtatagact tctgctgcct tttctgctgt ctcgatcaaa ccaaagactt
    32161 catccatgtc ttttcctgac ccgtagattc catgttgtgg ccataaaacc aatcgagttt
    32221 ctttcatttt ttcagctgtt gcttccccaa tctcatttgt tcccggaacc atccacggaa
    32281 tgatggaaat tccttctgga aaaacaacta agcattccgt acacatttgc catagcgtac
    32341 gactaaagga acgttcgtct aattcatgcg taaaactcat cgctaataga tgactagcat
    32401 ggcaatgcat aacgatccga ttatctgggt ctacttctaa acgggcgata tgactcatga
    32461 aatgacttgg caactcgctt gtaggaaggc attcgttttc cagcccccac aaaagttcca
    32521 gtgttttgcc atcttcagca actcgaacga ttccaagatt ctcggcaggt tcgctcgcta
    32581 catttttaaa atatcttcct gaaccagtta caataaaata gcatcctgct aaacggctgg
    32641 catcaaaaat catcgagatg cggcggatca ctttttcagg atccaagaac ggtgtgatct
    32701 cttcttcttt tagtaaataa gaaatattcc cgccatttct ttcatcccat cccaagcgat
    32761 aaagattcga cgtcgtttcg atcatttctt tcacataagg cgcttgtaaa atatctattt
    32821 ttttcaatat agccacactc ctattttctt aattccaaca cgtctttttg atattcctgg
    32881 acttcttcca gccaatccaa accaactgga acttgatttt tgtaacagaa ataattccat
    32941 acatcggcat acgggaaatc tttcaattct tccgtataag ccattcttga agtaaagtca
    33001 aaagccagct ccatttcttt caatcgctca actggttcca gcatagcttt cagcagtgct
    33061 ttttgtgtat ttctcgtacc gatgacccaa gcagcgatac ggttgatcgt tgcatcaaag
    33121 aaatccagac cgatatttgt tttttccaac aattcgttac ggactaattc tttagcgatt
    33181 tcctgcaatt catcatccat caaaaccaca tggtctgaat cccaacgcac tggacgggaa
    33241 acatgcaaca tgattccttt gctgaataaa gctaatgaag ataatttatt ggaaatcact
    33301 tctgttggat gaaaatgtcc agcatctaga caaacaagtt tgtcacgagt caacccgtaa
    33361 cccatataaa attcatggga accgactgta taagcttcag cgcctaaacc aaatagtttg
    33421 ctttctactg catcttgtac ataccgttca tctaactctt ctgtaaaaat ttcatcgagt
    33481 gattccatta aacgttcccg tggggtaaaa cgatcaatcg gattatcttt catcccatca
    33541 ggtacccaaa agttattgat acttgtttgc ccagtttctt tcccaaagta ctccgcgatt
    33601 ttgcggcttc ttttcccgtg ttcgatccag tattcacgaa cttctttatc tggatgagcc
    33661 aatgtaaagc cgtctttcat cattggatgc gaaaagaaag tcggattgaa gtcaagaccg
    33721 atttcttctt tttttgccca ctctacccat ttttcaaaat gtttcggctc aatttcattt
    33781 agatcgacag attcttctgt atccaaatag atagcatgaa ggttcaattt gtgtttgccc
    33841 ggtatcaacg aataagcctt ctccagatct tgtctaagct cttcaggtgt tcgtgcggct
    33901 cctggaaaat tccctgtcgc catgattcca ccggtcaatt cgccatccgg tgtcaagaaa
    33961 cctttgacat catctccttg ccagcaatgc atagaaattt tgatgtcctg tatcttttct
    34021 agtacagcat ctgtatctac accgatagtt gcatattttt cttttgcttg ttcgtaattt
    34081 ctttcaatcg tgttcatacg attactcctt tgattttatt cacgtaccat tccatcgtgt
    34141 tttgcttatt ttcatctggg taaaaagttt cacatggaaa cgactgtttg attacatttc
    34201 ttgcttgttc tatattcgaa aactgacctg tactgagcat ttgcatggca acattcccga
    34261 ttgctgtggc ttcactaggt cctgcttcta caatgattcc ggcataatct gctgtcaatt
    34321 gattcagtag acgattattc gatccgcccc ctacgatgtg cagcttttcc aatttcgttc
    34381 cagttaagtg ttctactttg atcagctctt gtgcgtaaca aagagcaaga ttatcataca
    34441 cacaacgagc gatagctccc gatgagacag ggactggctg ctctgtttca aaacagtatt
    34501 gctgaatctc ttctatcata tttgggggat tcaaaaatcg agggtcattg atttccatca
    34561 cttgctgcag cggtttttct tttgcagcaa gctctgccaa ttccgcataa ctgtactcgt
    34621 actcttgcat tcgggcaact tcctggatca accacatccc catgatgttt ttcaaaaaac
    34681 gcaccgtgtt atgggcaccc cattcattcg tataattttc agaaaaagca ttgcgagaaa
    34741 cattggcgat ctttgtttca atccctaaaa gcgaccatgt cccactgctg atataacccc
    34801 aacgttctcc ttgacctggc gttcctaata cagctgacgc cgtatcatga cttgctactg
    34861 tgataaaggt cacatctggc aaatcatatt ttgaaaattg ttctttttgc agatctccta
    34921 gtatcgttcc cggctctgtc aatggtggaa ataattcctt cgagacaccg attttttcaa
    34981 gaagttctat ttcccattct tttgtccctg cattcagcag ctgcatagta gatgcattag
    35041 ttttctccgt caccatgtta ccagtaaaga cataccctaa ataatcaggt atcagtaaca
    35101 atttatgtgc ttttgccaaa ttttcttttt cttcagcaaa caattggaac aaggtattga
    35161 aaggctggat ctgaatccct gtctgctcat acaacttatc tagagaatag atttcagaaa
    35221 actgttctac tgctccattg gtacgcttgt cccgataagc aatcggatca tttatccgat
    35281 ccccttgttc atcaatcaga cagtaatcga ctgcccatgt atcgatcccc gcaaaaaggc
    35341 gatcaatccc tgtctgcttt aatttttcaa atccaatcaa aatctctttg ataagataat
    35401 cgatcttcca gcgatcgtga ccgtctttgt tccgaaagcc gtttttgaac cggtgtactt
    35461 cttccaagat caaagaacct tttgcatccc tcgtactttt cattagtcgt ccgcttgatg
    35521 caccgatatc gatggcaaca aatgttggat tcatttctcc acctctttta ctttattgtt
    35581 gattagcact ttattaataa agcgttatca tttattgaca atgctatcat aatacagaat
    35641 cttatatttt gcttcacact attttggtat ataattgcat tatcttgaca agttgggagt
    35701 gtttacatgg attactggga aaaagccaac cactcatggt cggaagactc aaaaagagtg
    35761 atcttgacgc ctacacaaag aagcaggaat ttattttttt atatccaaga aatcggttac
    35821 ttcaaagcac caatccctta ctatactgaa cgagcacatt taccttctta cttgataaag
    35881 tttactttgt ctggcaaagg ttcgcttaca tataatgata atcagcaaca gctaaaagct
    35941 ggagacttat tttttattga ctgccaagaa tatcagcgtt atcaaacgat cagcgaagaa
    36001 ccatgggaaa tggattggat ccatttgaat ggtcagaacg tccaatcatt ttatcaggag
    36061 ttcacaaaag acggccataa caccttccat tcaacaggag atccatcaga aaacaagatc
    36121 caccatttga tcacagaatt gatccacatg caacaaggca cgaatgcccg taccgatttc
    36181 cagtcctctg tagctatcca cgagttgctg aatgaactga ttttgcaaaa gtatcagcta
    36241 gatttcgaag aagaggatat tcccgcctac gtttacaaac tgaagcaata cttagacgaa
    36301 catttcaaag aaacgattac acttgatcaa ttagaacaac aattcttatt gaataaatat
    36361 cagctgagca aagatttttc caaatttatc ggttctccac caatcgatta tttgatcagc
    36421 aaaaaacttt cttacgctaa agaccttctt cggtatacag aatttacggt tcgaatgatt
    36481 tccgaagaaa tagggattga gaattttgct tactttagcc gcctgttcaa agcaaaaaca
    36541 ggactatcgc cgagagactt tcgtaaattc agctgattca tataatatgt acgtcatttt
    36601 ttcaaa
//
LOCUS       DAZKCE010000012        36455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000012 DAZKCE010000000
VERSION     DAZKCE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.012"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..579
                     /locus_tag="ACG8KQ_000566"
     CDS             259..579
                     /locus_tag="ACG8KQ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9103535.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            733..1638
                     /locus_tag="ACG8KQ_000567"
     CDS             733..1638
                     /locus_tag="ACG8KQ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103536.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1635..2408
                     /locus_tag="ACG8KQ_000568"
     CDS             1635..2408
                     /locus_tag="ACG8KQ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9103537.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2589..4013)
                     /locus_tag="ACG8KQ_000569"
     CDS             complement(2589..4013)
                     /locus_tag="ACG8KQ_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9103538.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4224..4670
                     /gene="nrdI"
                     /locus_tag="ACG8KQ_000570"
     CDS             4224..4670
                     /gene="nrdI"
                     /locus_tag="ACG8KQ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9103539.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4735..6717)
                     /locus_tag="ACG8KQ_000571"
     CDS             complement(4735..6717)
                     /locus_tag="ACG8KQ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9103540.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6731..7627)
                     /gene="murQ"
                     /locus_tag="ACG8KQ_000572"
     CDS             complement(6731..7627)
                     /gene="murQ"
                     /locus_tag="ACG8KQ_000572"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9103541.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7630..8691)
                     /locus_tag="ACG8KQ_000573"
     CDS             complement(7630..8691)
                     /locus_tag="ACG8KQ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9103542.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8902..9429)
                     /locus_tag="ACG8KQ_000574"
     CDS             complement(8902..9429)
                     /locus_tag="ACG8KQ_000574"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9103543.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9496..10998)
                     /gene="eat(A)"
                     /locus_tag="ACG8KQ_000575"
     CDS             complement(9496..10998)
                     /gene="eat(A)"
                     /locus_tag="ACG8KQ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9103544.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11256..12377)
                     /locus_tag="ACG8KQ_000576"
     CDS             complement(11256..12377)
                     /locus_tag="ACG8KQ_000576"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9103545.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12503..13444
                     /locus_tag="ACG8KQ_000577"
     CDS             12503..13444
                     /locus_tag="ACG8KQ_000577"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9103546.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13594..14490)
                     /locus_tag="ACG8KQ_000578"
     CDS             complement(13594..14490)
                     /locus_tag="ACG8KQ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9103547.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14759..16099
                     /locus_tag="ACG8KQ_000579"
     CDS             14759..16099
                     /locus_tag="ACG8KQ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9103548.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16317..16760
                     /locus_tag="ACG8KQ_000580"
     CDS             16317..16760
                     /locus_tag="ACG8KQ_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9103549.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17264..18682)
                     /gene="nhaC"
                     /locus_tag="ACG8KQ_000581"
     CDS             complement(17264..18682)
                     /gene="nhaC"
                     /locus_tag="ACG8KQ_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9103550.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19085..20308)
                     /locus_tag="ACG8KQ_000582"
     CDS             complement(19085..20308)
                     /locus_tag="ACG8KQ_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9103551.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20339..21178)
                     /locus_tag="ACG8KQ_000583"
     CDS             complement(20339..21178)
                     /locus_tag="ACG8KQ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9103552.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21195..21875)
                     /locus_tag="ACG8KQ_000584"
     CDS             complement(21195..21875)
                     /locus_tag="ACG8KQ_000584"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9103553.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21877..22950)
                     /locus_tag="ACG8KQ_000585"
     CDS             complement(21877..22950)
                     /locus_tag="ACG8KQ_000585"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9103554.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23006..23244)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23287..23520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23597..24268)
                     /locus_tag="ACG8KQ_000586"
     CDS             complement(23597..24268)
                     /locus_tag="ACG8KQ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9103555.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24355..27003)
                     /gene="mgtA"
                     /locus_tag="ACG8KQ_000587"
     CDS             complement(24355..27003)
                     /gene="mgtA"
                     /locus_tag="ACG8KQ_000587"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9103556.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27345..27519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27692..28129
                     /gene="ndk"
                     /locus_tag="ACG8KQ_000588"
     CDS             27692..28129
                     /gene="ndk"
                     /locus_tag="ACG8KQ_000588"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9103557.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28151..29017)
                     /locus_tag="ACG8KQ_000589"
     CDS             complement(28151..29017)
                     /locus_tag="ACG8KQ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9103558.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29177..31270)
                     /locus_tag="ACG8KQ_000590"
     CDS             complement(29177..31270)
                     /locus_tag="ACG8KQ_000590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9103559.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32019..32642)
                     /locus_tag="ACG8KQ_000591"
     CDS             complement(32019..32642)
                     /locus_tag="ACG8KQ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103560.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32801..34198)
                     /locus_tag="ACG8KQ_000592"
     CDS             complement(32801..34198)
                     /locus_tag="ACG8KQ_000592"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9103561.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34230..35201)
                     /locus_tag="ACG8KQ_000593"
     CDS             complement(34230..35201)
                     /locus_tag="ACG8KQ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103562.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35575..36000
                     /locus_tag="ACG8KQ_000594"
     CDS             35575..36000
                     /locus_tag="ACG8KQ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103563.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10549 a         7602 c         6389 g        11915 t
ORIGIN      
        1 attaataaat aacaacttct ttctatgaaa ttgcaaagca ttcatcttct tttccgctaa
       61 cgatatatcc gtcagtgctt ggtacacttt actaaaaagt atgagattgc cgatatctgg
      121 ataggtgtgc tatactacag aaaaagatat acaatctgcc accgggtagg ataaagagga
      181 tgatcattcc attaggtctt tgaagggatg attttcctct ttttgtgtat ttttttatag
      241 ttaaggagga atgtaagtat ggcatggatg gaattaatca tagcaggaat tttggaagta
      301 ttctggtcaa cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac
      361 acagtgattg gcatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgccg
      421 atgagtctct cttatcctat ctggacaggg attggcgcag ttggttcggt cataattggt
      481 gtggtcttgt ttcatgataa gctgaatctt tccacttggt tctttgtggg gatgctactc
      541 atcagtatca ttggcatcaa gattactagc ggccactaga cacttggaat aagtactgat
      601 cagaaaaaat aagccttttt atttaatatt caattttttt atgaaaaaat aagaaataag
      661 tccaatgacc tagcgacttc tccactcatc aggtgtatgc ttgtactatc tatagaaaag
      721 gagtgaatat ttttgaaaag tctttcggtc caagaattat ccaagaactt tggtcacaaa
      781 caagccctca gcgatgtcag ttttagctta tcccctggca aaatcgttgg attagtaggt
      841 cctaatggcg ctggaaaaac aacgatcatg aaagctattt taggactgat ccattactca
      901 gatggaacaa tctgtattgg tgaaaatgag atttctccct ctagtcataa agggttggaa
      961 caagtaggag cattgatcga atacccgggt atctatccat tcttaacagg ctatgatcat
     1021 ctgaagttat tctctgaaac caacgatgta tctgctatcg acactatcgt gaatcagctc
     1081 aagatggaaa agtacattca caaaaaagcg aaatcctact cgttgggaat gaaacaaaaa
     1141 ttgggaattg ctcttgcctt actgaaccat ccgtctatcg ttattttaga tgagccgatg
     1201 aacggtttgg atccacaagc aacaagagat gtgcgaaaaa tcattaccga cttggctact
     1261 cagggcacct ctttcttgat ctccagtcac ttactcagcg agcttgaaaa aatggtcgat
     1321 gagctgatcc tgatcaatca aggaaagatc atcaaacaat gtacaatggc tgaattgaac
     1381 caaacagcgc aagatttcat cgttatctct acaacaaatg atctagctgc tcgaacgatc
     1441 ttgacagaag ctggatatac tttggaagat cctgataaaa tcaagctttt aaaagaaaac
     1501 gaagaattat taggaaccgt gatccagcta ttgtctgcac aaaatatcca tgtgacagac
     1561 gtccagcatc tgacgaatga tttggaatct tctctattgg aaattctgga ttcagtaaag
     1621 ggggaagaag aaaaatgaca ttgcttgtaa aacaagaatt atttaaattg atcaagaaaa
     1681 agtcaactgc tgttttatct gttctattag ttgttttgct cataggaaca gcacttctag
     1741 ccaaaaaata cacgacgatc attgatccag ttgaaatgac tgctcagctt tttagtgcca
     1801 catcttggat tgtctttatc atgattgcag ctgcaagcac gatcatttcg atggaagcac
     1861 aatatggtac attgaaaaat ctcctttatc gaaagtattc tcgaggagaa atattagtaa
     1921 gcaaatggat cacattagtt atttattctg tttatttata ccttttagct attattgtca
     1981 ctgtgttgat gaaactgatt cttttccctt cgatttcctt cacggagaag gtttctactg
     2041 gtcaaacact gatccaaagc ttgttcactt atactttggg tagttatatc ggtttatggc
     2101 tgattttaag tttagtgttg atgattgctt gctttatcaa tagttccggc gcatccatct
     2161 cagcaggaat cgtcttttat tttgcttcgt caatcatatc tggtatcctt attgcactca
     2221 tccaaaaatg ggagtggatc aaatggaatc cgatcagtat gttgaatctg caaaaccaaa
     2281 ttggaaacga agagatcatg aagcaattga ctcatctttc tactaatcaa atgctattcg
     2341 gtaatttagc ttacatcgtt ttgttcttag cattaggata tctcgtattt aagcgaaaaa
     2401 atgtatgatg actcatcaaa aaagaggctg tgaaagagac gttcagcctt attgccgatt
     2461 ggctgtctct taaacaccgt ttattctgaa aaaggagctg tgacaacatt tgtgtcacag
     2521 ctcctttcct agtatgtttt tgaataaacg acaggctcac aacctctctt tatacttctt
     2581 ttatacttct atacgtgaag catcccagtg gcaaataaaa ccattgcaat cactccgacc
     2641 atcgtcacac ctataagtaa aatcttatct aagtggatat gttgtacagg ctcgttttgc
     2701 tccttacgtg ctttgaggta aaaataaatg cctggcaaga aaagaacaag aacaagcatt
     2761 acttcattaa aggctacgac tagcatagcc catacttgaa aggcacttgc taaaagccct
     2821 actaaaaatt ggacccattc tttccgctga taagaaagtt tcatttggta caatcctaag
     2881 aaaagccacg tgaagaaaat agctgctgta ccaagtgtgt aagcaaagtt ataagcactg
     2941 tcagtgaata ataatgaaag taaaaagaca ttcgtacata acgtcgtcaa tacgatagca
     3001 taagttggtg ccccattttt gttgagtttt ccccatgctt taggcaaaag tccgtcttca
     3061 gccatgtttc ccattgtttc aatcggtaag atcgtccatg agagccaaac accaactaaa
     3121 gaaatgatca aaccgccgtt gatcacgact gctccccatt taccaaccat catttcaaac
     3181 acatagccca tagcaggctg cgccttgatc gtttgcagct gattcatcgt cattgtccca
     3241 tatggaagca tggaaaccaa gacgtaaata ataatcaagc cgaatatccc tagaatcgtt
     3301 gctttcccaa catctgattg tttttttgct cgtgaaccta cgacagaggc tccttcaata
     3361 ccaacaaata cccaaagcat caccatgatg caactcaata cttgactgga aaacgggatc
     3421 ggctgtccat tcgcattcaa tataatgtcg ctagaaaagt tatcaatgaa ttgttggatc
     3481 gaaaacgtgc tgatagctac tacaataaat aaaaataatg gcactaattt acagaatgtg
     3541 acgatagcat tgacgaaact agcattttcg actccccgat tcactagcca tgcatatccc
     3601 cataaaaaga ctgtactcat cacgatcgat gccagatttt gcccaccttc aaatactggg
     3661 aagaaatatc ctaatgcgct catcaacaat gttgcaaaag ctacgttgcc taaccagcat
     3721 gtgagccagt aaccccaacc gctgataaaa ccagcgaaac ggccaaaacc ttcttctgcg
     3781 taactaaaga tcccgccttt taaatctgga cgttttttat tgatattatt gatcgacaag
     3841 gagagcatca acattgctgt tcctacgagt acccaactga aaatagctgg gccgccagcc
     3901 gatccggaga tgtccgtcat aataccaaat acgccacctc caatcgctga accaattact
     3961 aaggcagcaa gtggaattaa accaattcct tttttctgtt gctctgtatc catgtaagca
     4021 tcctccaaaa taaaaccgct tttatcaaaa agtataacaa acctttttta tttttactta
     4081 attaataaaa actttaatag aaatataatt ctaccggctt ttttctcatt atacaagcaa
     4141 agcgcctagt ccctttccaa attacaccac caaaaaactt aaaaaagatg ctatactaaa
     4201 aggaaagaat tggaggatga tccatgaaca ttttatatat atcgatttca ggaaatacgc
     4261 gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat gcatgcagaa gatcctgtca
     4321 atcctgaagt tactttaaaa gaaatccatg aaaatagtga ttttacgaaa gaaacagaac
     4381 cattttttac tttcgttcct acttatttag acggcggaaa tggtttagat aacggtgata
     4441 cagaaatctt gaccgaaacg atgcgtgaat atctggaaca tgaagacaat cataaacttt
     4501 gtcttggtgt agtcggtagc ggaaataaaa acttcaataa tcagtattgt ctaacagcca
     4561 aacaatatgc acaaacattt ggctttcctt tcttggcaga ttatgaatta cggggaacac
     4621 catctgatgt gacacgaatt tatcagattt tagcagaaaa caataaataa taaaaaagaa
     4681 gaagctgaaa aatagatttc agtttcttct ttcgtttcta ttgtttctat catattaagc
     4741 tttgacatcc atcacaggtg cttttgcttt tgctttacct gttttttcca agacaaagtt
     4801 ttttacacga tccatattgg ttaccaagac gatcatggat gtttctttgc ctgctgcttt
     4861 gatcgcttct aagtcagctt ctgctacaag cgtatcaggt gtcacttgct gcccttctgt
     4921 tactttcaca tcaaatggcg taccgcctaa gtcaactgtg ttgattccca tatgaagcaa
     4981 gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt ttcgttggga agacagagct
     5041 gactacccca gtaactggag aatagatatg tccgttttct ggttctacaa agtagccttc
     5101 acccatcatc ttttgtgcaa aaactggatc acttgcttta tctaaagaaa caactgttcc
     5161 gtcagcaact gatgagagga tagtcacact ttcagctgaa gtttgaggta ctactttttc
     5221 aggcacttca tttactgtgg ctgtttcagt cgctaattct tctttttcta aacggtcttt
     5281 aggaataccg aagaagaatg tagcaacgaa gccaccagca taagctgcca ataatccaag
     5341 gatgtagcct aaccatttac cgtcagagat caatgggatc aaagcggctc ctgaaggtcc
     5401 aatagcgata gccccgacat ttccgatcat tccgatcact gccccgccga tacctccacc
     5461 gatacaagca gtaataaatg ggcgtcctag aggtaatgtt actccgtaaa tcaatggttc
     5521 gccgattcct aggatcccga ctggtaaagc tcctttgatc atttctgtta attctttatc
     5581 ttttttgcag cgaatccata atgccaatgc tgcacccact tgtcctgcac cagccatcgc
     5641 aagaattgga agcaatagcg tacgaccagt ttcatcaatc atttgcacat ggattggagt
     5701 caaaatttga tgcagtccga acataaccat cggcaagaat aatgtgccta gaacgaagcc
     5761 ggcaaatgct ccaccaacgc caagtaccca gttgatccca ccgaccatgc catcagaaat
     5821 gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga ccgatggcaa tcaatgaaag
     5881 agttggtgta acgatgatat ctaccgcatc aggtactact ttatgcagtt ttttctcaac
     5941 gattgacaat agccaaacag cgaagatgac acctaggatc ccaccttgtc cagctgcaag
     6001 agcttcacca gtaaataagt ttttcagtgg tgcttccgga ttcatccctg tcagcatcac
     6061 aacggccccg ataactccac caagagttgg tgttgcacca aaaacttgtg cagcattgat
     6121 acctgtatag ataactaggt agctaaacat cccgtttttc aagatgttca tcacatcgat
     6181 atattgttgc catgttgctg catcaagtgt tccggctgtg actaagtttg ctaaaactgc
     6241 ggcaatccct gccacgatcc cagtaccgac aaatgcagga atcattggca caaagatatt
     6301 agaaatgtct tttaataccg atttgatttt agatggtttt tgtttcgatt tttgtgccgc
     6361 tttcatttct tgtgcccgtt cgtttacacg gtcttttcca gatgctgatg aatttgctgt
     6421 agctgtactt acagaagaaa gtttttctcc taatttcacg cctgccaaat caaccatttg
     6481 ctgtgctact ttgttcactt tcccaggtcc gatgatgact tgaagctgtt catcttctac
     6541 tacaccaagc actcctggaa tcgcttttaa ttcagagaca tttacaagat catcattgat
     6601 gatcttcata cgcacacgag tcatgcagtg gacaatgctt tctacattgc ccatgccgcc
     6661 tgccccttta taaatttgtt ttgccagttc ttccaaagtc agttcgtttt ttgccatggt
     6721 cttttccccc tcataaggtc ccttttacaa atccgtttgc ttcagctaat tttgcttgag
     6781 cttcctcttt atcactattt gtcaaaatca tgacgatagc cagcttaaca ttttgatctg
     6841 cttcttcaaa gtacttttca gctgtttcgt aatcagcttc tgtcgcttgc atgatgattc
     6901 gttttgagcg ttcgattagt ttttcatttg ttggtttgac atcgaccatc aagttattga
     6961 atactttacc tataccaatc atactgatag tagaaatcat attcaagata agtttttgtg
     7021 ctgttcctga cttcaatcga gtagagcctg tcaaaaattc tggacctgca tcgacttcga
     7081 ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt gcaagaaatc gttcctgtga
     7141 cagcaccgat cgattttgcg taatctaatc caccgatcac atatggtgta cgaccgctag
     7201 ctgcgatacc aataactata tcattttttg tcaaattcag cttgattaga tcttcttgcc
     7261 ctagctcata agaatcttcg gccccttcga cagctacagt cattgcttct tctccaccag
     7321 caattagacc tatgaccatt tccggttcta caccgaatgt cggtacgcat tcagcagcat
     7381 ctaacacacc tagtcttcca cttgttccgg ctcccatata aatcagacgt ccaccttttt
     7441 taaaggactc aatcgtgtta gcaatcactg gttcgatact tgtcagttct ttttcaaccg
     7501 cagcggccac tttctgatct tctttgttca ttagttgaac ggcttgagaa atactcatct
     7561 catctaatcc aaaagtttct tggtttctgc gttccgttgt taggttctct aaattgattt
     7621 tgctcatttt tatcttcctt cttttcttat aaattgatag ttcataccag ctttgatttg
     7681 gttgatcaat ggtagctctt ctttgataac tttagctact acattcactt tttcatccgc
     7741 tggcaaatca tatttggtta attggatctc acccatatag cgcaaatact tttcgttatc
     7801 taaagtaata gaccctttca tacgcgtagc tgtatttctt gctgggatag gcggaatctc
     7861 tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca tcgtcttgcc gattcgtatg
     7921 cttccctaat atatattcgt aaaattcttc atctacaggt tccgtatgga gcaagactgt
     7981 ctgttctttt tggaaagaag caaattgctc ctgaacgttt tctgacagac ctgcatctcc
     8041 gatgtaaaca atatccgtgt tacttgctaa tagatctaga gcgcctgcaa gaggatggat
     8101 ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt gggcctctca actctgtatc
     8161 tcctggtaca aaaccttgga caaggagacc ttgaattttg agccattgat tttttttctg
     8221 ataccattct ttgtccagcc ctgtttcggg acgtggataa taattatgcc aagcttcaat
     8281 ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata tctttaggcg tgatcgtgct
     8341 agcattcaaa ctgattttca attgatgtga ccagtcggca atctgctgat tggaaatatg
     8401 ataatccatt cttaaacctg ttacaccaat tgctttcaaa ggcgttagat cttctaatga
     8461 aaaacccgct cgttccaatg cctctcctga aatatcgacc atcaattcta gtttattctt
     8521 tttcgcttcg cttcctagtt cgatcaatcg cttttgatat gccgaaacat catcttctgg
     8581 tatatgcatg gaagtgaaaa taccgacaaa gccattggat gccatttggc ggatatactg
     8641 tttcttctct tctgacaaag aatcgttcat aaatattgaa aagccaaaca tattatccct
     8701 ctttttctgt atccgtcttc atttctatga ctagagtata tatctaaaga aacaaaattt
     8761 caatactttt attaaaataa ttaaatttca tttctaaagg ttgagtttat attatcaaaa
     8821 gaattgtcat aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat
     8881 ttaaataaca caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa
     8941 tgtaaagaac ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc
     9001 attatggcaa actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg
     9061 aacgatccgt gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc
     9121 taaacgtgct ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc
     9181 catttctacc aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg
     9241 tgctgtatct cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa
     9301 atctactacc ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac
     9361 atctgcccgt ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac
     9421 ataaagcatg tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc
     9481 ttgagattac aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag
     9541 gaaggtttga tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc
     9601 taattgttct tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc
     9661 agctggctgt gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg
     9721 ctcgatcttg ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa
     9781 tgcttggtag tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc
     9841 ataattttgg cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac
     9901 cttgccgttg aatgccccca gaagataatg gataatggat gactttcctg caccgttagg
     9961 acctgaaatc gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag
    10021 attttcatac cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg
    10081 gctattcatc gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct
    10141 gatctgggtc tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc
    10201 agcacccact gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt
    10261 ctttgtttta tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt
    10321 tagcctactg acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag
    10381 ttttttctgt tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc
    10441 gattgctaaa acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat
    10501 gaagccttgt tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa
    10561 atgattcgtt ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag
    10621 taggactttt gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg
    10681 atctgtttgc atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc
    10741 atttaacacg taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa
    10801 ttcttgctga tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa
    10861 agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt
    10921 tagatttgct tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt
    10981 tatttcgatt ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa
    11041 ctggctaaag aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc
    11101 aagccaatcc cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc
    11161 agctttcatc aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg
    11221 aaattacgat agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat
    11281 ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct
    11341 tcttctaaac ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt
    11401 ccttctagct cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt
    11461 gcaagcactg ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct
    11521 tctcctgtaa tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg
    11581 actaaatcga aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg
    11641 cctgctgctc cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc
    11701 tgagcaatct ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt
    11761 tgtcttccaa attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca
    11821 gcgataatca attttacttt agaaacaaga acgtttcttc gatcaaaatg aagtttttta
    11881 gtagataata aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt
    11941 aatcctagac caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact
    12001 tcttgaagca gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata
    12061 gtttgatccg ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact
    12121 gcggtgtttt ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag
    12181 agatccatcc catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca
    12241 gtattttctc cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag
    12301 ccatttagaa ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt
    12361 gccgccaaaa tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat
    12421 ttttttcaaa caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca
    12481 ttgaataaag gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag
    12541 ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc
    12601 tgctcacatt gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt
    12661 ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga
    12721 cttgatcgta gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc
    12781 cattattact gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt
    12841 actggaaaaa tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg
    12901 tctagctggt cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt
    12961 ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt
    13021 aaatccgatc tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg
    13081 aacattgaat ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa
    13141 agcggcagag agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga
    13201 aggaatcata ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga
    13261 caacatcggg ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac
    13321 tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac
    13381 accatattgt gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga
    13441 ataaaaggct ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc
    13501 aataaaagat caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt
    13561 ttcagtacat aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc
    13621 atattcgtat tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc
    13681 tgcatcataa aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc
    13741 cggtaattca tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg
    13801 ataatgagca atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa
    13861 tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc
    13921 ccctgttttt ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg
    13981 tggaagaatg aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc
    14041 tgctcgttct tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct
    14101 agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt
    14161 tgcttccaaa aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt
    14221 cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt
    14281 ggtaaatggg cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat
    14341 cagctgataa tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc
    14401 ttttccggac caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt
    14461 tgcaactagc agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt
    14521 tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga
    14581 aaatttaaca tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct
    14641 aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat
    14701 attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat
    14761 ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg
    14821 tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc
    14881 ttctgtattg attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct
    14941 gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc
    15001 ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat
    15061 caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat
    15121 tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc
    15181 ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat
    15241 cttactgatc atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt
    15301 ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat
    15361 catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac
    15421 ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg
    15481 aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa
    15541 tactttagca ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc
    15601 atttgcagcg gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc
    15661 gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc
    15721 tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac
    15781 cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta
    15841 tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc
    15901 ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt
    15961 agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt
    16021 attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt
    16081 cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg
    16141 agctattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac
    16201 agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt
    16261 tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga
    16321 aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga
    16381 tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg
    16441 cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg
    16501 aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct
    16561 tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc
    16621 ctcatcattc tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa
    16681 aaaaattagc ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa
    16741 tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag
    16801 ttatcagctt caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg
    16861 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt
    16921 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
    16981 gtaaaatcag cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat
    17041 tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt
    17101 tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat
    17161 ttttgtataa atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa
    17221 agggattgtg acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg
    17281 ctgtcttgct tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga
    17341 aggcactgac catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag
    17401 tgagtgtagg aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta
    17461 agtagttgat tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc
    17521 ctgctttttc ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt
    17581 caccaataaa aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg
    17641 tgttcgtcat ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca
    17701 acaatccacc taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga
    17761 ttcctccgcg gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg
    17821 attcgatgac cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc
    17881 ctactgctac agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac
    17941 acagtaacat taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta
    18001 gaacatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa
    18061 tcatgaataa aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga
    18121 tgtgtttgaa caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg
    18181 gagacatctt atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat
    18241 ttaagcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa
    18301 aggcactccc tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc
    18361 actggacact gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta
    18421 tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt
    18481 tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat
    18541 acaacatgac cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga
    18601 caacgccaat cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac
    18661 ttggtgttcc tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa
    18721 aaagttaaga tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat
    18781 aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca
    18841 gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca
    18901 attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac
    18961 tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa
    19021 gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac aagattattt
    19081 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct
    19141 tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt
    19201 ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt
    19261 ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga
    19321 cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt
    19381 cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag
    19441 taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac
    19501 ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc
    19561 gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta
    19621 ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag
    19681 cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg
    19741 ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag
    19801 attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct
    19861 caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc
    19921 ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc
    19981 tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg
    20041 gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac
    20101 gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt
    20161 ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt
    20221 cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca
    20281 attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt
    20341 attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta
    20401 ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt
    20461 catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt
    20521 ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga
    20581 tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa
    20641 tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct
    20701 tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa
    20761 catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt
    20821 tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt
    20881 gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc
    20941 catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa
    21001 tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa
    21061 tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc
    21121 ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt
    21181 tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt
    21241 aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga
    21301 ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg
    21361 gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa
    21421 ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc
    21481 gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac
    21541 ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact
    21601 aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg
    21661 atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg
    21721 cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc
    21781 caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa
    21841 acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt
    21901 gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa
    21961 ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc
    22021 taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata
    22081 tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc
    22141 caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa
    22201 tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt
    22261 gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac
    22321 cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc
    22381 taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag
    22441 tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat
    22501 tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc
    22561 caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc
    22621 agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa
    22681 atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg
    22741 actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat
    22801 cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc
    22861 tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa
    22921 acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc
    22981 ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg
    23041 ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag caagatatcg
    23101 gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc
    23161 aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg
    23221 tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta
    23281 tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa
    23341 gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt
    23401 gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc
    23461 tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga
    23521 tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa
    23581 attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa
    23641 gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga
    23701 caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc
    23761 ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag
    23821 ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg
    23881 tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag
    23941 tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg
    24001 catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct
    24061 tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac
    24121 tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa
    24181 attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac
    24241 catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca
    24301 tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa
    24361 gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc
    24421 gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc
    24481 agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac
    24541 aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg
    24601 gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat
    24661 gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca
    24721 aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa
    24781 gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc
    24841 tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa
    24901 caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga
    24961 aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa
    25021 agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat
    25081 cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc
    25141 cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt
    25201 tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc
    25261 atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt
    25321 aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt
    25381 gtccccagtc agaactttta cagctacacc atgttctttc agagctttca acgcttgttt
    25441 cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc
    25501 atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac
    25561 tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt
    25621 tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc
    25681 aatcgcacct tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat
    25741 acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt
    25801 atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt
    25861 ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca
    25921 atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca
    25981 taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt
    26041 tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc
    26101 aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt
    26161 tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag
    26221 catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt
    26281 ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact
    26341 tccactgatg acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg
    26401 atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc
    26461 ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc
    26521 tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt
    26581 tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg
    26641 attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat
    26701 aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa
    26761 tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat
    26821 ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct
    26881 tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa
    26941 ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc
    27001 catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc
    27061 tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc
    27121 tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct
    27181 ttactaagta attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt
    27241 agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc
    27301 caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt
    27361 tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa
    27421 gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag
    27481 cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata
    27541 acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata
    27601 cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata
    27661 atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc
    27721 agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact
    27781 ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca
    27841 gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt
    27901 cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc
    27961 aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac
    28021 tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt
    28081 tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta
    28141 aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc
    28201 gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct
    28261 tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat
    28321 agatcactgt acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac
    28381 gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa
    28441 agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca
    28501 aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata
    28561 gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat
    28621 aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca
    28681 aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt
    28741 tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact
    28801 ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc
    28861 gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc
    28921 cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt
    28981 tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa
    29041 atttttccac attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa
    29101 aaatgttttt ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga
    29161 aggttggttt gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg
    29221 gatataagtt ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc
    29281 ctttttgctt ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg
    29341 gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct
    29401 tatctgaaag acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga
    29461 ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta
    29521 ggacggtgta tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct
    29581 gagaagataa taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat
    29641 gtagtaatgt cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag
    29701 tataaccagg catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat
    29761 cccatgtatc tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga
    29821 tcccaaaatg atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca
    29881 aatacgtaaa aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat
    29941 ctactgtgct attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat
    30001 aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa
    30061 gatattgggc agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt
    30121 cggattcatc aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat
    30181 gatctctatt ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct
    30241 tttgtccaag gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata
    30301 atgagccttg aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct
    30361 ttccctgtcc tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa
    30421 tactatttaa aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct
    30481 ggaaactttc taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg
    30541 gatttggctc tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc
    30601 ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg
    30661 catcgattcc tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg
    30721 gacggttggc ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa
    30781 cagcgatagc catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca
    30841 accaaaccag tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct
    30901 tcatcaaaga aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag
    30961 atttgactgt aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa
    31021 gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat
    31081 acaaagaaag gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt
    31141 actggatcgt accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca
    31201 gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa
    31261 gttgtgtcac gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa
    31321 tgaaaatttc ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc
    31381 cgtccacttt tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata
    31441 aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt
    31501 acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca
    31561 gctttttcac aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca
    31621 caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa
    31681 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat
    31741 tatgtcagga cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc
    31801 ggtaataagc ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg
    31861 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgacct
    31921 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa
    31981 tactcaggtc aaaacgctga tttcacaacc tctgtttttc attgtccata ggattgatat
    32041 tgtccttgtg atgactcatt cgtaataatt gaggcttgat tttcttcact tcccgttatc
    32101 gtttgtccac tgccagaatc tgaaggactt gttcctgtcg actcgttttt ttgagaagag
    32161 acagttgttt tcttgacttg ttcatttgtc tttttccatt ccgctaaacg atcggcgacc
    32221 acttttttgt ctactttttc agaataagcg gtgatcactt ttccattttt caaacgggct
    32281 actttgtcgc catcatgagt caaaagcaaa acggtcaaat gcttgtcttt aaattgatat
    32341 tgtctgagag aaatcgcact aagtacgtca tcgatattaa taaattgatt ttcttctttt
    32401 ccttttggca aataaatgtc caattcgcct ttttcgtaat tggcagttat tttatactcc
    32461 gcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt gcttgctacg
    32521 attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa tgctgcggat
    32581 aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct ttttttcctc
    32641 atgctttcct ccactcctat ttaattttga ctattctatt atatacaaaa aatgaaatag
    32701 aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcac aaaatggtaa
    32761 gaatggagaa tttcacgcat ttgctctcaa ctattctttc ttaagcctca aatccgtttt
    32821 tatctgatac gtctttgaac caatgacccg attttttgat tgttcttttt ccatcttgtg
    32881 ctaagtctac tgaaataaag ccatatcgat tcttatatgc attggtccac gaccagttat
    32941 ccatacatgt ccacatatga tagcctaaac aattggcacc ttcttgaatg gcttgatgga
    33001 catatttcag atgagattgg acaaattcga tccgataatc atcgtggatc atgccatctt
    33061 ctttgataaa tcgtgcttcg ttttctacac ccataccgtt ttcagaaata aagcagtcga
    33121 tatttccata atgatctcgt acattgatca aaatatcata gattccttgt tcatagatct
    33181 cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg aaataatcct
    33241 caggcattgg accgttttta gtatcgattg gtgtttcttt tgcttttacc cggcgtggct
    33301 ggtaataatt gatacctaaa agatctactg tattttcttt gataaccgtc aaatcctctg
    33361 catggatcac aggcatgaga tcaagttctt ttactatctc aatcaattca cttggaaact
    33421 cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct atttgcgctg
    33481 ctttaacatc ttcagggttt gattcgtctc ttggatagct tggtgtcaga ttcaagatga
    33541 taccaataac accaccttgc tccatttcat ggtaagattt gatggctaac gctgaagcca
    33601 acgcttcatg atagccaact tggatcgctt ttttcatatc tgactcttct ggataatgcc
    33661 atcgatataa ataacctgct tcgactggaa cgattggttc attatgagta aaccattttt
    33721 ttactttcga accaaacaat tcaaaacaac ttttggcata tttttcatag gcttcaaccg
    33781 tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg aaatgataca
    33841 gattcacaaa gggttcgata ccgttttcaa tcaactcatc aaaataacga tgatagaaac
    33901 gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc cattggatag
    33961 atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct tgatacttcg
    34021 tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc ggttcttttt
    34081 tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt ccttctgttt
    34141 gtgggccact tgctgcagaa ccccaccaaa aatttgctgg aaattgatat tttttcatca
    34201 aagcacctct ttttcttttt tagttcgttt tagttgattc catgtatgat attttccatc
    34261 acaatcactt tttcattttc ttcctcttct tttttgattc gttttaacag ctgttctgct
    34321 gctcgttccc cgattttatc tgttggtggc gtgatcgtga ttttcggaag gatactcaga
    34381 tcgtttttat ctaaataatc atagtgactg aacgcaatat ccttcccgat tctgacacct
    34441 tcttgattga aacattcgat ggcaccaatc gacatattgt aattgctgac aaaaatagca
    34501 gttgcccctt tttctaataa tgcttttgcc ccgtaatagc cgctttttct agaatagtct
    34561 cctgtaaaga cctctaattg gatcgatgga tcattttgga ccgcttcttt ggctcctgct
    34621 aaacgttctc ttgctacgta gtctgtttga gtggcagcaa taaatccgat ttttttatgt
    34681 ccttgttcac catgatgagc gatcactttt gctacacttt ctcgatcatt cactaatatt
    34741 gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga taactggaat actcattttt
    34801 gtgagcattt caaatccaga ccactcttct tcggataaga agacaatcag cccatcgatc
    34861 acatgcttca tcaggtattc aattttttgt tcaatttttt ctggctgatc ggcaaacccg
    34921 cttaataata agctataccc attttcttca gctactcttt cgatccctga gacaacttcc
    34981 gacccaaaac ggctggaaat cccgtttatc aaaagtccaa tagagaagct tcgattattc
    35041 ttcaaccctt tagcaatgat gttttcttta tagtccaacg ctgaaatcgc ttctgctatt
    35101 tttttcatgt tcttttcctt cagttgctga ccattcaagt aacgggaaac tgttcccact
    35161 gaaacaccag ccatcttagc cacatcacgg atcgatgcca tacattctca ctcctgccag
    35221 ttacatttga tttctctttc ttgttgttcg attatgatga ccgcattctt tgcatagtgt
    35281 tgcttgtcat caatatccat ataactaaag tcttcagtag agcaacttcc tctcactatt
    35341 aattatactt aaacaagtat ttgaaccggt tcatttttaa tatagcattt ttttaaaaga
    35401 ttgcaacact tttttaaaac gctttctgaa agtggcggat tttttgcctt tttaaaattt
    35461 tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc atccgtttga
    35521 agttttacat tccaatggta taatttcttt atcctatcaa atgggggaag tattttgaag
    35581 aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg ctgtgatttg
    35641 tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa
    35701 aaagcagata ccaccaacag tgtatatacg ctaaaagtac gtaaagatat ggatttatcc
    35761 gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta tcagacacaa
    35821 ggaactgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat caagatcact
    35881 accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaaac aacagtcata
    35941 tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg gcaacatcta cagtctatag
    36001 tgccgtctgt tggatcttct tttgtttctc cccttctttt ctgctatagt aaagaagcaa
    36061 gatgattgta aaggagacga aatggatgat cgatagtcct gattattgta catgtaataa
    36121 caacaaaaat atccatacag aatttaatga ccaaggaagc ttgagcatat gcgacaactg
    36181 tcacaaacta gtcaaagatt catttaaaac atttgaatct tatcctgttc aaaattatgt
    36241 aaaaagtaga agaagataaa caaatccaag caaaactcac tatggctgat ttttatgaac
    36301 ttcaaactca gcttcttcct ttgatcaagc ttcatcagta aaacaaacag tggatcattt
    36361 cagtttacaa atcaaagagt aaagatttat ttatttcaaa tatttgaata taaaataacc
    36421 aaacgataga aaggcttatt ctacatcacg tagta
//
LOCUS       DAZKCE010000013        36243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000013 DAZKCE010000000
VERSION     DAZKCE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.013"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..264)
                     /locus_tag="ACG8KQ_000595"
     CDS             complement(52..264)
                     /locus_tag="ACG8KQ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103564.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            451..918
                     /locus_tag="ACG8KQ_000596"
     CDS             451..918
                     /locus_tag="ACG8KQ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103565.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            915..1157
                     /locus_tag="ACG8KQ_000597"
     CDS             915..1157
                     /locus_tag="ACG8KQ_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFP9103566.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            1175..1477
                     /locus_tag="ACG8KQ_000598"
     CDS             1175..1477
                     /locus_tag="ACG8KQ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103567.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1549..3603
                     /locus_tag="ACG8KQ_000599"
     CDS             1549..3603
                     /locus_tag="ACG8KQ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103568.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            3603..6215
                     /locus_tag="ACG8KQ_000600"
     CDS             3603..6215
                     /locus_tag="ACG8KQ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFP9103569.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            6212..8743
                     /locus_tag="ACG8KQ_000601"
     CDS             6212..8743
                     /locus_tag="ACG8KQ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9103570.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            8795..9127
                     /locus_tag="ACG8KQ_000602"
     CDS             8795..9127
                     /locus_tag="ACG8KQ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103571.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            9128..9721
                     /locus_tag="ACG8KQ_000603"
     CDS             9128..9721
                     /locus_tag="ACG8KQ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103572.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9884..11578
                     /locus_tag="ACG8KQ_000604"
     CDS             9884..11578
                     /locus_tag="ACG8KQ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9103573.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            12266..14143
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_000605"
     CDS             12266..14143
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_000605"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9103574.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            14274..14651
                     /locus_tag="ACG8KQ_000606"
                     /pseudo
     CDS             14274..14651
                     /locus_tag="ACG8KQ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            14651..15856
                     /locus_tag="ACG8KQ_000607"
     CDS             14651..15856
                     /locus_tag="ACG8KQ_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9103575.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            15872..16516
                     /locus_tag="ACG8KQ_000608"
     CDS             15872..16516
                     /locus_tag="ACG8KQ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103576.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            16527..17333
                     /locus_tag="ACG8KQ_000609"
     CDS             16527..17333
                     /locus_tag="ACG8KQ_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103577.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            17355..17585
                     /locus_tag="ACG8KQ_000610"
     CDS             17355..17585
                     /locus_tag="ACG8KQ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103578.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            18131..18406
                     /locus_tag="ACG8KQ_000611"
     CDS             18131..18406
                     /locus_tag="ACG8KQ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103579.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            18448..18870
                     /locus_tag="ACG8KQ_000612"
     CDS             18448..18870
                     /locus_tag="ACG8KQ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103580.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            18889..20481
                     /gene="mobP2"
                     /locus_tag="ACG8KQ_000613"
     CDS             18889..20481
                     /gene="mobP2"
                     /locus_tag="ACG8KQ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFP9103581.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            20493..20828
                     /locus_tag="ACG8KQ_000614"
     CDS             20493..20828
                     /locus_tag="ACG8KQ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103582.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            20975..21169
                     /locus_tag="ACG8KQ_000615"
     CDS             20975..21169
                     /locus_tag="ACG8KQ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103583.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            21201..23573
                     /locus_tag="ACG8KQ_000616"
     CDS             21201..23573
                     /locus_tag="ACG8KQ_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9103584.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            23634..25094
                     /locus_tag="ACG8KQ_000617"
     CDS             23634..25094
                     /locus_tag="ACG8KQ_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9103585.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            25105..27309
                     /gene="topB"
                     /locus_tag="ACG8KQ_000618"
     CDS             25105..27309
                     /gene="topB"
                     /locus_tag="ACG8KQ_000618"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9103586.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            27325..27474
                     /locus_tag="ACG8KQ_000619"
     CDS             27325..27474
                     /locus_tag="ACG8KQ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103587.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            27548..28141
                     /locus_tag="ACG8KQ_000620"
     CDS             27548..28141
                     /locus_tag="ACG8KQ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9103588.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            28157..29056
                     /locus_tag="ACG8KQ_000621"
     CDS             28157..29056
                     /locus_tag="ACG8KQ_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9103589.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            29059..29544
                     /gene="ssb"
                     /locus_tag="ACG8KQ_000622"
     CDS             29059..29544
                     /gene="ssb"
                     /locus_tag="ACG8KQ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9103590.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            29910..30170
                     /locus_tag="ACG8KQ_000623"
     CDS             29910..30170
                     /locus_tag="ACG8KQ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103591.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            30611..30817
                     /locus_tag="ACG8KQ_000624"
     CDS             30611..30817
                     /locus_tag="ACG8KQ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9103592.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            30817..31068
                     /locus_tag="ACG8KQ_000625"
     CDS             30817..31068
                     /locus_tag="ACG8KQ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103593.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            31083..31520
                     /locus_tag="ACG8KQ_000626"
     CDS             31083..31520
                     /locus_tag="ACG8KQ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103594.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            31513..32220
                     /locus_tag="ACG8KQ_000627"
     CDS             31513..32220
                     /locus_tag="ACG8KQ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103595.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            32385..32585
                     /locus_tag="ACG8KQ_000628"
     CDS             32385..32585
                     /locus_tag="ACG8KQ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103596.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            32601..32780
                     /locus_tag="ACG8KQ_000629"
     CDS             32601..32780
                     /locus_tag="ACG8KQ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103597.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            33121..33384
                     /locus_tag="ACG8KQ_000630"
     CDS             33121..33384
                     /locus_tag="ACG8KQ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFP9103598.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            33378..33728
                     /locus_tag="ACG8KQ_000631"
     CDS             33378..33728
                     /locus_tag="ACG8KQ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9103599.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(33752..34366)
                     /locus_tag="ACG8KQ_000632"
     CDS             complement(33752..34366)
                     /locus_tag="ACG8KQ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9103600.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            34680..35102
                     /locus_tag="ACG8KQ_000633"
     CDS             34680..35102
                     /locus_tag="ACG8KQ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103601.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            35112..35315
                     /locus_tag="ACG8KQ_000634"
     CDS             35112..35315
                     /locus_tag="ACG8KQ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103602.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            35526..36110
                     /locus_tag="ACG8KQ_000635"
     CDS             35526..36110
                     /locus_tag="ACG8KQ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9103603.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        13464 a         5716 c         7377 g         9686 t
ORIGIN      
        1 atagtcaata aaaaataaga aaagccttat ataattttgg cttttcttat tttatttgtt
       61 agttgaagta ttaatatcaa cgatttcttc agccaattta tttaatttca tagcatttat
      121 gaatctcatg ttgtctatac tagagcgttt gttggcaagg tcacttactg tagttaaggg
      181 tacgccagat tctatggata tagaacgaac tgatttacca ctatcaaaca gcaaccattc
      241 gattttcttt ttatcagcgt acatttcaat gctccttctt gttttttctt tattatagct
      301 cttttttgta atgaatcaca atatcaagag aaaaattttc tatagaaagg tggattttta
      361 tgagtattcg ttctccgtgt atccaaatga ggtttgttgc tactttcgtt atttgatata
      421 ctaaaaataa acaacgaaag aaggaataaa atgaagaaaa agcaactatg gtttgtctct
      481 atgtttgcaa tattttttat tattttaagt ggatgttcaa gtaataagaa atcggaagct
      541 aaagaagata atagtttatt gcaaccagga actgtttgga aagaagaagt aggtggtctt
      601 attcataatt taaaaattat agatgatact acctgggaat attctgaaag tgtatggcat
      661 ccagatcctg tacaaataac agttaagagg caaaaagact atgaaggttt agaaagatac
      721 aaaatagtag attctgcagg cattagggaa tttataaaca aacctgattc attatttatt
      781 gttgttccct atgagaagga tggcgtaaaa aaaataattt ttcttggatc tagcaaggat
      841 gagaagcgaa cgaaagagaa attaattcat gatggatcag agttaaataa atacaaatta
      901 caaaagacta gtgagtgatt gctagtcttt ttatttatcc tgaaggagga atcacagtgg
      961 caaaagttaa aacaccattc gaaaaagccg cgtctaaaat cttagaacgc cgtggtgttt
     1021 ctgaaataca gtggaaaaaa gaagttcttg aaaaagcaca atttgaccta ttacgaggaa
     1081 aagataagga cttagaagaa tatatctatc aacgtgaacg tgaaaaactt gtattcgatg
     1141 aaattattaa acattaaaag gagcgaataa tcttatgttt gaatcaatta caatgaattt
     1201 tttaactcaa ccgaaagtag tagctgcagt tgatattttt agagcattac aaactgccgc
     1261 aactagtttg aaaaataatc tgatgatttt tgcggctgta gctgccgttg cctccattgc
     1321 tcttggaggt attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt
     1381 aggttatgta ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat
     1441 gttctcaatg tttggacaaa ccccacaagg actataaaaa aaacaagtag gaggagtaaa
     1501 aagtacgtgg ctacttttta ctcctttttt tatgaaagga gaatattcat gttttttacg
     1561 atttcaggaa atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga
     1621 aagcgtactt cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag
     1681 ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc
     1741 acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca
     1801 ctggattatc aaatttcaga aggtttttat cctacattga tagatttaat ccgccttgat
     1861 gtgtttaaag atacggatga aactatgagt gtggaagagt cttatcgaaa cgaaaaactg
     1921 ctacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgaatca agtgagtgaa
     1981 gtcgaagtaa atgaagaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa
     2041 gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt
     2101 gaggaagaga acgatgtgat gggtcaattg gaggtagatc cctttgaaga aacaatggag
     2161 gatgaagaga cggacgaagt agaagttgat ccagttgaag ctaatgaaga gtcaacatgg
     2221 actgaagggg attttcagga atatattgaa ccacttatca gtgaagaagt agaaaaaaac
     2281 gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag
     2341 catgaatgga tgaaagaaaa aaacaaatta tttttaaatg agctttattc gaaatatcaa
     2401 ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa
     2461 ggcacacttt tagaacagtt agagtctgtc caatcaacaa attggcatga gcttgcacaa
     2521 gaagatttat tagccacctt tgaagaacgt caacaagaaa cagatcaaga aattaatgag
     2581 tatgcgacgg aacagtcgtc taattgggag aaagtaaaag aggaactaaa taaagaagaa
     2641 cagcgagtga ttgaagaaga agtacaaaaa gtcaaagagc ggtacgaaaa gaaacgcgac
     2701 aatgcttatt ccttgtgtgt agaaaagatc aacaatttta ctaagacgaa tcaagaagcg
     2761 ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta aagaacgaaa agaagagttc
     2821 taccgcggtt tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat
     2881 aagctatatc aaacaacgac tgaagttctt atggaaaaac acgaacagat ccagcaagaa
     2941 ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa
     3001 gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgtg aacaattaga aataagaaaa
     3061 cgggaacagg atcttgcgga acaaaaaatg gcgatagaaa aacaacggtt agagcaagaa
     3121 aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgtcaa
     3181 gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac gccagtgaac
     3241 aaccaagcaa atttaactcc ttcaccccag cgttccttta acggatggat ggtgggatgt
     3301 attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat
     3361 caatcttcac aggctgattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa
     3421 caagcaagaa aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca
     3481 acaacagagt caagcatgga aaatagcaaa gagagtgatc ctagtaaggc taaagaagga
     3541 tcaagcgatt caactgtaca ctcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa
     3601 taatgcgtat tagagaaaca acgagacgaa aagacaaacg attcaattta gaaaagaata
     3661 ggtatctttc tcatttctat cgctatactc cttcaggaga agagctacca gaaaaacatg
     3721 acaaacgtat ttctcaaaag attttttata gtggcttagc ttatcttcct ttaaactatg
     3781 ttgttacaag aataccagaa gcatttcatc gatttgagat tattccagga agtttacaag
     3841 gagtgaatat ttttcagtta caagaaatgc cgcagttatt ccattttgat tcaactggtt
     3901 tttggaatcc tttgacccca acaacaagtt taggcacacg tttagttgtt tctacgattt
     3961 gtgttgttcc agctgtactt atgggattga aaatggacat gtggtttcgt ccgctagcag
     4021 atggacaaaa aggggataat cgattaatga ccttaaaaga aatccaacag atttatccgc
     4081 aaatacctga aacaaaaact acttttccag gttatggagg tattccaatc acacattaca
     4141 aaaaatattg gtacattcaa acagatactg ttaatacgtg tattgttggt acctctcgat
     4201 cagggaaagg tcagattgaa gtgttagcca ccattgacaa tttatctcgt gcagaaaaac
     4261 aaagttcaat ggtcgttaat gatccaaaaa cagaattata tgttggtagt aaagatgaac
     4321 tagaagctag agggtatgat gtatatgcct ttaatatcac cgatccactg caatcaatga
     4381 gtgataatcc attggcactc atcgtgaaat actggaaacg aggagacata gatactgcaa
     4441 cacagttaac caatactttc acaaatacga tttattatga tgccaacgct tcagataaca
     4501 aatactttaa cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac
     4561 aagctgatca aacaggagat tactccaaat tgacaccgaa taatctcgtt gagcttcttt
     4621 ctgagattgg cggttttaat tattatccag atccagacaa tgaattcaaa cagttgaatg
     4681 ccttagatga atttatgaat cagttgccac ctggaaacat tgcaaaaaaa caatatgggg
     4741 caacgaaaat tggatctgat aaagcgaagg gaaatatttt atcaacggca atcacgggat
     4801 tgaatccatt tactctaacg aaaattgcaa agatgacttc tcaatcaacg attgattata
     4861 aatcggttgg gtttccaaaa tacttagatt tgaaattaca tgaaagtctt ttaaatcaac
     4921 gagtcgtagt agaattttat cataagggaa agaaatttca tgaagaacgt gtcaaagtag
     4981 gctataaagg atttagtgaa atcaattttg attgtcattt agttgcaggt gatcaagttt
     5041 gtttaaaata cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt tctcaaagag
     5101 tattgaaaga cgaagagggt aatatcgttt atcggaaaaa acgaggagaa acccatttgc
     5161 cagaattaga aaaagaagcc gttctaacat tagatgagga aaaaagtaac ttattagtag
     5221 agtctattcg gatgcactat accgataaac caatggctat ttttatgctt acacctgatt
     5281 atgatccatc taaccatatc attgttagta tttttcttag tcagttatat aaagaattat
     5341 ctacgcaatg tgttaagact aaaggagata aatgtcaccg caggattcat tttattttag
     5401 atgaatttgg gaatatgcca ccaatggata atatggaagg aatcatgacg gtcacagctg
     5461 gccgaaacat gttgtgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg
     5521 gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga
     5581 cagatgataa taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag
     5641 agaacagtac catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag
     5701 atcgtatctt aactcctgaa agaatttcta cttttttaga aggagaaacg attgtactcg
     5761 ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc
     5821 atggggaaac gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg
     5881 atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa
     5941 ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg
     6001 atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tcttttgaga
     6061 atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcagca gatcgacttc
     6121 aagaatatgt tgaaaagttg atcaaaaata aagaaatttc tgaaatgatt gggaaacggt
     6181 tgttattgga atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat
     6241 agccgactgg tggtcaggct tattcggaga aggggatgca aaccccaaaa ccgtaacgaa
     6301 agtcttacaa gattatggga attatctgca gtatcaagat gtatttagtt ggatttggaa
     6361 cacttttctg tggggattga ttaaaggatt atataatctc aatactttac tagaaaaaac
     6421 catttatcaa agctttgatt taaaagactt attgaacgcc gctggggtaa atgcactatt
     6481 tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg
     6541 cttgaaattt tttatgagta aacacccacc aaaagtcaaa aatatcttgg ttaatttatt
     6601 tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag
     6661 taaaagcttt tacggagacg tcactgcagc aaataaagca gataaaacgg gatcacctgc
     6721 ttttcaaatt attcaaaata atgtgatcga tgtcagtacg ttactagcaa ctgacccgaa
     6781 taaagtagaa tccctaccaa cagataaaag aaatgcttta acagcgaaaa attttaaaac
     6841 agcgaatatc aatgggatta ttactcctga acaagctaaa ggtatggcag gtaatgataa
     6901 aagtgggtta tcagatatac aaaaggatcg gttaaaggca ttacaatacc gattggaact
     6961 agatgataat gggaaagaaa ttcctgttca aattagtgac gaaggcttag caaaatatgt
     7021 ctatacttca gggtatcgtc gatacagtag taaaccaggt attattttag ctggtgaatc
     7081 ttctttggca gtagcctatt tatttatcct atttacgatc gttacttgca ttattgagct
     7141 ggtatttaaa aaattctatt tagtgattgc cgcgtcaacg gactttgaga caggtcaacg
     7201 gatgaaaagt gcgatagaag atatttctca atccttcttg ttattggcgt ttacgatttt
     7261 agaattacga atctatacct taatgctttc aggtatcggt gatttgcatg cggcaggaaa
     7321 gatcaatgga ttcttgtatg tcgttgcttt gattgtacta accattgcgc tattcaaagg
     7381 ctctcaatca gttacgaaaa ttttcggcgt tgatacaagc ctgaaaaacg gcagtaattc
     7441 tcttcttagc atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa
     7501 ccttgcaagt gctggtaaga gtggtttaag ccatttaaat gatattcgta aaaatggatt
     7561 tggccatcga aaagatgtta cccctgataa cggggaagaa caatcagacg ataaagctac
     7621 aactgtacct aatagtaaga aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt
     7681 ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagctga
     7741 agatcttaca gaaaaagcca aagacacctt agaagataaa ttagacggca cgagagctaa
     7801 agaattggct gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc
     7861 ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa ggacaagtca atgcagcagt
     7921 gaaagctaat acaacaaccg aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa
     7981 agatcaccaa gaagcgttgc actctaatga aaattcgatt gaagagaaaa attccataga
     8041 agaaagaaat tcagaagaag gaaaaaatgg acagttacca aaaactgatc cagaacaata
     8101 tcgctataaa cctaacagtg accttgaaaa tgagaagcga atttcagatt tatcaaaagt
     8161 aaatcaagaa gacaaagcag aaaatgaggg acagttacca aaaactgatc cagaacaata
     8221 tcggtataga tctgttaact ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa
     8281 tcaagaagta caaacggatg aggattccat cgttctgcca aatgataatg gaatcgaagt
     8341 tggaactgaa aaatcaacga aaattggttt acctaaggca caaaatttac caatgaaaga
     8401 tagtcgtttg aatcaattca aggataatga tcaaagtttt gttcatcaat cacctgcagg
     8461 tactgttcaa atgaaaccaa gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta
     8521 caatttatct gaaagtggtg agacagggaa tgcgtccgtt caaaatgttg tgacaagaga
     8581 agctgcacca aaacctattc aaaacgttca tacaggttac aagccagcaa atgtggcatt
     8641 tactgcacca acaaaaataa atgtgacaag agaaacaaaa aacaggaaca atgcattgaa
     8701 agaacaacta ttgagggata tcaaacagga tatcaaacca taaaaggcct acaagttagg
     8761 ccttttttct atgtatgaaa aggagaaatc atttatggat aaatatagta acatgaaaaa
     8821 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
     8881 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
     8941 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
     9001 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
     9061 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
     9121 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
     9181 aaaaccagaa gagcgctcta tcttggactt atttccatat aaatcctgta cagaagaggg
     9241 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
     9301 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
     9361 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
     9421 aatcgtgcaa aagcggcaat tattagataa aaatcgtatg gaacagtcct taggaagaaa
     9481 tctacaacga ttaaaaaaat atgaaaagaa attagtcgaa gaaatccagg aattgaaacg
     9541 agcagaacaa gaacgtcctg acttaagctt ctttttcgtc attgaaggaa agacaatcaa
     9601 ggatttacaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
     9661 actctacaaa aaagaattga tcaagattct gtatcgaatg aacaatatga atgatgagta
     9721 ggaatggaga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaagg
     9781 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaagggcaat
     9841 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
     9901 aacagctttt tcaccacaaa atgatcaaaa agtacttgat ccattaaagc aactaggaaa
     9961 tcgggaaata gaaattgtaa aatccaaagg gtatgattta gattttttaa aacaagtgat
    10021 gccacaaggt gggttaaatt ttgatgaaga atatgtgacg tatggtaatg gttattcacg
    10081 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    10141 gaacaatcct catacagtgg ccacactcga tgttagcaca gatgaaaagc aaaaagtgat
    10201 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcga tcagataatg aacgaaaagc
    10261 gactgaccga aataaaagcg cggaagaata cctggaacta gcggaatatg ctagaaaatt
    10321 gacttctaat ggggaaattt ctaagcaagt caaagtgaga gtacatgttt acggaaacac
    10381 gattcaagaa gtagatgaac gagcgcagga actgatcgaa aatctcaaag ggatggatta
    10441 tcgtgcggtg acttatctgc ataaaaccaa acgagattgg caatcgatgt ttaccatgtt
    10501 cgatgaacaa gctgaatggt tcggaaatat caaagggcaa tcactaccat cgttaaacat
    10561 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggaggga cacaatatgg
    10621 ggaaaccatg acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt
    10681 gtctactaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    10741 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    10801 ctttattcct ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg
    10861 aatgatcaat cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata
    10921 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacacactt
    10981 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    11041 agaatttaag acctacttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    11101 tacttcctca aattcgcgtt caaatactga tacgcaagtc acagggaaag atccagaagc
    11161 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    11221 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    11281 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    11341 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    11401 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    11461 aattaaagaa aaacaaatgg aagaacaaga cttcaaacgc ttcttggcga tgattgatga
    11521 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    11581 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    11641 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    11701 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    11761 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    11821 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    11881 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    11941 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    12001 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    12061 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    12121 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    12181 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    12241 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    12301 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    12361 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    12421 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    12481 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    12541 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    12601 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    12661 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    12721 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    12781 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    12841 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    12901 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    12961 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    13021 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    13081 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    13141 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    13201 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    13261 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    13321 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    13381 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    13441 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    13501 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    13561 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    13621 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    13681 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    13741 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    13801 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    13861 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    13921 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    13981 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    14041 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    14101 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    14161 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    14221 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    14281 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    14341 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    14401 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    14461 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agtgggtaaa tcaatgacga
    14521 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    14581 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    14641 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    14701 aaagcggcgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    14761 cactgtgttc gtgaccctat ttctaatact tgcggcgggc tcgattgata attcggattc
    14821 agattcaagc agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    14881 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ccgtaggtgc
    14941 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt acgaatcagc
    15001 gtttggtaca tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    15061 tggttgtctg tttcctaaag gaactgcacg aggaattggt ggcattgaag gtggctggta
    15121 catgaagttt cccaactcaa aagctgcatt ttcctattac ggctttatgg tagccacaca
    15181 atccaatttt aatgcttgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    15241 acgaggcggt tatgccgctg caggaattac ggaagatagt ccgtattata caggttgtat
    15301 gtcaattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    15361 tgaaggtggc aataacaacg gcacgattac tggtgaatgg acgaatccat tccctggcag
    15421 cagtttagat aaaagttcct tcagcggtgg tcaattattt ggaacgaatc caggcggtga
    15481 attccgtccg aacggcttcc atgatggatt agattttggt tcagttgatc atccaggaag
    15541 tgagattcat gcggttcatg gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    15601 tttgggtgcg tgtgtgatcg tgattaatta cgatggcttg aatatggtgt atcaagaatt
    15661 tgcaaatagt acaggaaatt ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt
    15721 aattgggata cgagatacag cacacttgca tttaggattt actcgaatgg attggcgtca
    15781 agcacaagga catgcattca ttgatgatgg tacatggatc gatccgttac catttttgaa
    15841 tagcagtaaa aaatagatca ggagaaaaaa tatggaaaat aaaacgttaa aaatcgtctt
    15901 aacagctgca attagcgtta gtttaatcgg caatattgcg ttgttttggc agctgaaaga
    15961 ggaacaagca gcaaatacct ataaaattga agaagtggct aaaaacgcgt ctgagaagct
    16021 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    16081 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    16141 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    16201 aagcagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    16261 attaagtcct acagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    16321 aggtgacgtg caaacttacg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    16381 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    16441 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    16501 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    16561 aaaaacgaaa aagttatctt agatggctgg gaatctttat gattctcggc tttttgattc
    16621 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    16681 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    16741 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    16801 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    16861 tgattaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    16921 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    16981 cacaagatct gattttttat attcacgaag ataaggtcaa agatcacgtg aaagacgaag
    17041 attatgaaca acatgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    17101 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    17161 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    17221 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    17281 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    17341 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    17401 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    17461 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gataccttaa
    17521 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    17581 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    17641 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    17701 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    17761 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    17821 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    17881 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    17941 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    18001 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    18061 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    18121 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    18181 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    18241 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    18301 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    18361 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    18421 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    18481 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    18541 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    18601 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    18661 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    18721 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    18781 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    18841 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    18901 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    18961 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    19021 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    19081 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    19141 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    19201 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    19261 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    19321 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    19381 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    19441 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    19501 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    19561 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    19621 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    19681 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    19741 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    19801 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    19861 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    19921 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    19981 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    20041 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    20101 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    20161 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    20221 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    20281 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    20341 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    20401 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    20461 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    20521 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    20581 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    20641 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    20701 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    20761 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    20821 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    20881 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    20941 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    21001 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    21061 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    21121 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    21181 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcggttgg
    21241 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    21301 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    21361 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    21421 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggct
    21481 ataacaagta cggcggactg gatatgacga tcgataatat tccaagaatc aaagaagaaa
    21541 tcgaacgctt tgaaaaaggt gaatcgactt tttcagccgc cacgatccga aaatatcaac
    21601 ggaagttgga aacattagaa cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc
    21661 tccctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aaaaatccaa
    21721 ccatttattt tttgaaaggc tatcgaaaag ttgctttaga actgaatgaa aacgggggat
    21781 ttgaggaatc aaaaaaatac caagccacgt cggaagagga aaaagcagct gttcgtgaat
    21841 tacttgaaga ggctgcacca actgattctc tgaaggagaa agaaaaagat catttctatc
    21901 gagtagaatt caacgaaaat gatccagaaa aaggattgaa aagttatggt ggggagatcg
    21961 taacattaga attgatcgat caacttaaaa cgttggataa ccaatacgct gcagaagcag
    22021 gttattataa attctatttt gaagaagtga agaacggaga agtagtagat cagatccgaa
    22081 tggatctagg ggatggcgtc aacagtaatc accccatcta tttagagtta gcaaaattgt
    22141 gcgaacaagt acctgagaag gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag
    22201 aacaaatgca gtcgaaagaa acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag
    22261 aagagatcaa acaagaagtt aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc
    22321 atacccattc ttctgaagac gtcaaagaac ttttgaactt tatgtctcgt tttccagaac
    22381 gatcctttag aaatcaatta ttgattgagg aacaatttcc aggtgcaacg gcttgtttag
    22441 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggggaacaa gggaacaaga
    22501 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    22561 cagaagctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    22621 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacagtta aaacctgaag
    22681 actatccgaa aatctttccg aaccgtgtct ttgattttca attggataaa aaaggacagt
    22741 cagaacttca agctgggatt caagcggtag ccaacaatat tggaatacaa attcgagata
    22801 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tatgttcgta
    22861 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    22921 tagctacaag tattcatgaa ttagcccatg caagaatgca caaattttca gaattagata
    22981 ccgcaactaa agaattgcag gcagaaatga cttcttatat tgtgtgcaaa cattttggaa
    23041 tggacacttc ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt
    23101 tggatgataa ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa
    23161 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    23221 tccaaccagc agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga
    23281 atattgtgcc cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag
    23341 atacgggtcg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    23401 aagtgatttt aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag
    23461 aatttgcgct aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac
    23521 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt
    23581 aaaaaagact atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg
    23641 aaaaaaaaat gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa
    23701 acaatggaat ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat
    23761 ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca
    23821 ttattgaatt agctaaactt tttttcatag acaaagagat ccaagactct aagcaacaat
    23881 ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc
    23941 ttcattttga aacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa
    24001 aaggaaggaa ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag
    24061 aaaaagaagg gttgattgcc gaactaaagc caaaatatga acggcaaaat ttcttagttc
    24121 gagacgatcg tttagatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga
    24181 cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca
    24241 catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta aagatcggtg
    24301 accctaagca gttaaaattt tttgagagta gtattgattt acttagctat gctgcactga
    24361 atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga
    24421 tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat
    24481 ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat
    24541 taatgggcgc agtcgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta
    24601 atgattggca agtgccaaaa gaatacaaaa tcatttatga agaagtcgca aaagaagaaa
    24661 aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg caattaacga
    24721 accaactggt ttccgctcac aaagtaaatg cgttttttgg ccagcagctt tctgttaacg
    24781 attcaataga agccattaat ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag
    24841 tttgtgaacg cgtggatggt acgtacgatt ttgatcgttt ttatcaagaa aaaggggata
    24901 ttaatgcgca gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc
    24961 atgaacatga atttatacct gaagtaaaaa aggactggaa cgatcaattg aagcatgaga
    25021 tccagcaaca agaaatcaga aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac
    25081 aagaaaggga gtgaaatcgt catgatgaaa accgttattt tagcagaaaa gccgtcacaa
    25141 gcgaaagctt atgccgatag cttttctaaa gctacccgaa aagatgggta ttttgagatc
    25201 caagatcgat tgtttacagg agaaactgtg atcacgtatg gttttggtca tttagtcgag
    25261 ttagattcgc ccgatatgta cgatgaaaaa tggaaacaat gggcattaga acatttgcct
    25321 attttcccca atcaatacca ctatcatgtt cctaaagata agaaaaagca gtttagtgtc
    25381 gtcaaacgac agctgcagtc agctgatacg attgtgatcg cgactgatag cgatcgtgaa
    25441 ggggagctga ttgcctggtc aatcattcaa caagcaggcg ccaatcaagg aaaaaccttt
    25501 aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt ccaacagtta
    25561 cgagacgcag aagagaccta tccaaaattt gaagaagtac aagcacgtca gatcgccgat
    25621 tggttaattg ggatgaacgg tagcccactg tatagtttac tcttgcagca aaaaggcata
    25681 ccaggaagtt tttctttagg tagagtccaa accccaacac tctatatgat ttatcaattg
    25741 caggaaaaga ttagaaactt ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc
    25801 gctcaaaatg gcgcatttga tgcgaagctt gatccaaatg aaacccaagc gacacaagaa
    25861 gcatttgaag cctatttaaa agaaaaaggt gttcaattag gaaaacagcc aggtacgatc
    25921 cttcaagtcg aaacagagaa aaaaagtgcg gctagcccac gtcttttttc tttatcgagc
    25981 cttcagtcta aaatgaatca actcatgaaa gctagtgcca aagatacttt ggaagccatg
    26041 caagggttgt atgaaggaaa atatctaagc tatccgcgaa cggatacccc ttatattacg
    26101 gaaggggaat atgcgtatct gttggatcac ttagacgaat acaagcattt tttaaaagcc
    26161 gaagcgattc cgactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
    26221 caggaacatt acgcgatcat tcctacaaaa acggttatga cagcagcggc ttttgaacaa
    26281 ctaagtccac tgcagcaggc tatttatgaa caagtgttaa aaacaacggt ggccatgttt
    26341 gcggaaaagt atacctatga agaaacaacg atcttgacgc aagttcagca gcttcagtta
    26401 aaggcaatcg aaaaggtgcc gctagatcta ggttggaaga agttatttgg aaaagagagc
    26461 gacggaaaag agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg
    26521 acggtggatc tacaagttct tgaaaaagaa accaagccac cacagcctta taccgaaggt
    26581 acgttgatca cggcgatgaa aacagctggg aaaaccgttg atagtgaaga agcgcaatcc
    26641 atattgaaag aagtggaagg aatcggtaca gaagcgacac gggcgaatat tattgaaacc
    26701 ttgaaacaaa aggaatatat caaggtagag aaaaataagc tcgtggtgac gaataaaggg
    26761 attttgcttt gccaagccgt ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc
    26821 aaatgggaaa gctacttgtt aaagatcggt gaacgaaaag gaacacaaac cacttttcta
    26881 gccaatatcc aaaaatttgt ctctcactta ttggaagtcg taccagggca aatccaatcc
    26941 actgattttg gatcgacgtt acaagaagtg aaagcagcca gtgaaaagca agaagccgcg
    27001 cgtcatttag gaatttgtcc aaaatgccag gaacaagaag tcttactcta tcaaaaggtg
    27061 gcagcatgta cgtctgaagc ctgtgatttc aaactatgga caacgattgc caaaaagaaa
    27121 cttacagcga cacagctgaa agagatcatt caaaatagcc gaacaagcca accagtgaaa
    27181 gggttaaaag gtcagaaggg ttcgtttgaa gcaacgattg ttttaaaaga agacttcact
    27241 acaggctttg aattttctga aaagaaaaaa acgaacttca agaaaagaac acgacgcact
    27301 acaaaataat ggagggttaa tcttatgcgt aatacaacac acaaacaaca agaaatcgaa
    27361 aatcaaaaag ccaaaattcg ttcagaacag gaaaaaatgc gtgccagcaa acgaagactg
    27421 cagcaattga agaaccagca gcggattcaa aaagaaatcg aattagatcg ataaattcct
    27481 ttcttgttaa tataaaacta acatgttata gttgtgttag ttttatatta actttagggg
    27541 aagagtaatg aaagacaaaa tccaatgtat ttttgataaa cataatggca acctatcctt
    27601 gaaggatgct agaagtgaag gattatcgcc tatgacgtta gatcgattag ccgaaaagga
    27661 taaaatagaa aaggtagcac caggtttcta taccttaaag gatgcgttcg tggatgaatt
    27721 gtatctcgct cagagtatct attccagagg gattttttct catgagactg cgctcgatct
    27781 ttttcaagta ggaacatata tccctaaaaa gatcaatatg atgtttccta aaggatacaa
    27841 tgtatcaaag gagaaattag atcagtatgc cattcaaccg cattacacta ataaagaaaa
    27901 ttttgacctg ggagtcacag aaacagaaag cttttacggc aataaaattt tagtctatga
    27961 tttagagcga acgttatgtg acatgtggaa tccaagatac aaagcaagcg tagaggtaaa
    28021 acaagaagca ttaaaagaat atatgacaag taccagtaga aatacaaata agctaagaag
    28081 atacatggat attttaaaat caccaaaaga aatgactttg tatatgttgc cactttattg
    28141 agaggagtaa agatgaatga caccgacaca atttaaagag aaaacaagaa atttcgcagc
    28201 agagaaacat ttaaacccac aaattgtcca aagatattat atgatggaaa aattattaga
    28261 atgtattgtt gaatcgcctt atcatgatga ttttgtatta aaagggggat ttctgattgg
    28321 atcaaaatac gggttagaaa accgttcaac cgttgatatt gatacgactt atcgaaattc
    28381 gaaactaaca aaagaaaagt tagaaactgt tttttctgaa ttaacagcta atcctacaaa
    28441 agatggaatt cgttttgaat taaagggatt aacagagacg agggaagccg attattatcc
    28501 aggttttcaa gcaaagttta ttgcttatct tgaaaatgca cgtgtacctt ttaaattaga
    28561 tattacttca ggtgattcta ttattccagc agcattgatg tatgaacaca aattaatgtt
    28621 tgaagataaa acgattcata ttccagctta tcctacggaa caaattctag ctgaaaaatt
    28681 acatgccacg tttagttttg gaaaagataa ttcgcgcatg aaagattttt atgacctgta
    28741 tatgattccg aaattagaac gtatcgatga gaaaaatttg tataagagtg ttcaaggaac
    28801 attcaaggaa agaaataatc aatcggtgtt taagcatttg tatgatcagg aaatgcctca
    28861 attggtgaaa gatgagggct tgagagaaaa gtggaaaaga taccaacaag aaaactattt
    28921 tgccaaagac atttctttcg aatctacact aacttccgtt gattccctga tgcaatcaat
    28981 tgtagagcaa gagaaggcag aacgaaaaca aaattttaag agatcacaac aaatgaatca
    29041 agaaatggag agataaagat gatcaataat gtaactttag tagggcgatt aactaaagat
    29101 ccagatttac gttataccgc aagtggcaca gcagtggcga ctttttccct agccgttaat
    29161 cgtaatttca cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg
    29221 cgcaaaccag cagaaacgat ggcgacatta gctaaaaaag gcagcttgat cggcgttgtc
    29281 ggacgaattc aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa
    29341 gttgtggccg ataacttcca actattagaa agtaaagcag cgacagaaag ccgtatgcac
    29401 gctgatcaat cgtccagcag tccaagccct accacctttg aacaacggga tacagcgacg
    29461 cctaacaaca atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt
    29521 agcgatgatg atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta
    29581 tgttataatt tagacaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg
    29641 ggctagtggc tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt
    29701 cttggcaaat ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat
    29761 tgcaaaaaca aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag
    29821 gccggggtag cagacagcaa gactattata gcataattga aatatccaaa tgattggatt
    29881 tttgctataa ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa
    29941 tccgagtaat aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat
    30001 cgattaattg tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg
    30061 gaaaatacaa tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta
    30121 gcatgtatgt tagaaatccc gatcatttta cgcgatcgat gggcatataa ataaaagaag
    30181 agttgtagtc ctaaatgagg attgcagctc tttttgtttt acatagaaac aggaaaagac
    30241 ctttttagga agacacaagt gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc
    30301 accgccgttc gagaagaaag gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc
    30361 aaagcaagac aaaatagaca aaacttcctc tcaccaaaag gttcgttcct gttcgttatc
    30421 tctttattga tttgccagac cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga
    30481 caagggtaaa tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga tttattctgg
    30541 ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct aagaggaggc
    30601 agaatcaaac atgcgaaaga aatttgatta ttggggtgtt cccttcagcg aattattccc
    30661 aaactatcat gcaccacata ctgttgaatg cgattgtgga gaaagagcga aatgtatcaa
    30721 aagctatcgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt cttatggcga
    30781 ttacattttg attgatgaga aaggaaagaa acgctgatga aaacacaaca caaattattg
    30841 actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca
    30901 gaagctttac ttattccgcg gtactggacg atagaaaccc ctttagtaga ggatgcaccg
    30961 actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta tcaagaaaac
    31021 atggaaactt atgaaatcga atattggtta accaaattag agaactaaaa aggagaaaaa
    31081 atatggctca aaattactat gacgaatttg tcaaattacc tttggataaa atggcacaaa
    31141 aaatggagga tatgacgttc ctataccatg aaacacgtgt acccaaaaaa cattacaagg
    31201 aaaaattgtc agtcgcagtg gaagaaatga ttgaatcagg tgtcgaaatg aatctcattg
    31261 cgacgtacta tcgaacactg gaagaactga aaaaacaaaa tggaaaatgg tttttccaag
    31321 ccttactatg cttggaagta ggtgtcaaac caagcactat caaaccttcc gaatatcaag
    31381 ccttagaact gacttatgca aaattcgcac agattaagaa agctaaagca atggctagcg
    31441 actggctaga tacttttgaa agtatcgaaa aaaatggtgc attttatacg attgaatcgg
    31501 aggagaaaga taatgaataa ggtggtttat cagctaacaa cagaagatgt ttcgcaattt
    31561 ttaacaagca aagaacactt gaatgaagta atcagacgga ttcaaaaaga ctttagttgt
    31621 atggaagaaa tgaagtactt tattcaaaga gtggaagaac agttgtatat ggaaagcgaa
    31681 acaagtcaaa cgaaggtcac agtcagccat aatcatttag acgtgtataa acgtcgtcct
    31741 gaaacggtaa aaaaaggaac agagcttcta cgactagaaa cagaaaaata catcatgaat
    31801 gtgtacctag aaaggagtga gctggtcgaa aactttgtca gctacacgat ttgtaggaaa
    31861 cactattact cagaatcaga atattattca attttcgaga gcatggaatt aatggaagaa
    31921 gaaatggaag agctaaacag caaaaatgcg acggtagacg attattacct tgtatttact
    31981 aggtttttga cgtgtgggac ggaatctatc gtgttaatgg gaatggatta tgaactcgct
    32041 gtacccgaag catttttgag ggaatatgta gaagaagtag ataacccagg cgacgactgg
    32101 tacacgactt taactgattt tttagacacc tacacttacg acgatacaga agaaatccaa
    32161 aatgaacttg accgtaaacg aatgccatat caacgatttg aaaggaaaaa aggtaaataa
    32221 ccatttatac cgaaaagtga ggatgtgtga aataagaaca aaaaaccttg aaattaaggg
    32281 aatggttctt atttcgcacc aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat
    32341 aagcgggcaa agcccgataa cctaagagaa agaaggagat ccatatgaat aaaatcattc
    32401 ctctattgtt cgtactgttt gttggcgaga tacttatgcg tcatgctgca gctgtacatt
    32461 taaacttcgt agtattctta ggaatactgg tattttgttt gatcttttgg cgatttgtat
    32521 tagcccttgt gagtctacta ttcaagatga ttgcagccat atttttcata gggttatttc
    32581 gatgaaagga gagagtacta atgccaattg caataggaaa taaacggtta cccgtgacat
    32641 tagatgaaaa gagacaaaaa gaattgcagc aactaaagca gaagtacgga aaaagtgaat
    32701 ctaagattat gtgtattgcg ttagatctat tgatcgcgca agaaaaagca ggatttgata
    32761 taccagcact aaaaaagtga cgtcactttt catttgaaga aacaaaagtg atagcactta
    32821 attgttccta ctatttagaa tattttctat gtccttttta ttccattttg tatcaataac
    32881 tatgaaatct ttaaagtatc cagaagagat tatttttaga tcaatttttt tcttacctga
    32941 ttctaaagat tttgttttct taaacatatt atgatttcct ttcgactatt ttcatataaa
    33001 tgttaacata gtttatggat tcatggaatg ttaatctaat ttaatagaaa aggataatta
    33061 agtatatacg ttttttctta tgtatgttat actatgtata tacagaaaga ggtgtctact
    33121 atggaaatgt tacctataaa aaaatgggga aatagtaatg gccttagatt gccaaagtat
    33181 attatggaat atcttggaat tcatacagaa gacaaagtga agataataca agaagaagta
    33241 aacggtcaga agcggctaat tatcgaagcg gctaatatag aaaatgaatt aacaattgaa
    33301 cagttatttg aaaattacac tgatgaaaga aatcatgtga cgattcaaaa tttaggtgaa
    33361 gcagtaggta atgaaaaatg gtgagaaaac ctagacaagg cgatattcta ttattgaata
    33421 cagcacctcg atctggacac gaacagacag gtaaaagacc ttatattgtt ttaagtcatg
    33481 atattattgc ggattatagt aacgttgtta cagttgctcc aatttctagt acaactagaa
    33541 actatccgct atatgtaaat ataaactcag aatatgaaat gaagacaaca ggaaaagtat
    33601 tattagatca actaacgaca attgattatg aagccagaga gtgtattttt ttggaaaaag
    33661 cgcatgatag tttagttgag gagttattga tgaaagttag aacagttttt caaaaagtaa
    33721 gcaaataaaa tagaaacagc agcatgatca actattcgtg attgaccatg ctgtcttttt
    33781 ttattctata tacggtttgt ctcgtaacat tgtagtcttt tgcaatctta gcgatcgcaa
    33841 cacctttttt tagatcctca acgatgtttt tataaacaag acgacgttgg ggatcttttg
    33901 cattggcact gtatagctta ggacgtcctt tataaacacc gttcgctttt gcaatcttaa
    33961 tgccttgtgc ctgacgtcgt ttagattcgg tacgttcttg ttcagcgatc atggccaaaa
    34021 tttgaatgat caaatcttta atgaatttat ccaggagagg attgccaatt gcttcggcca
    34081 tgatcggtag acttgtgatg atcagctgca catttttctt cttcaagtaa ttgactgaag
    34141 cgatgatctc gtcgtaattt cttccaagtc gatcaatagc ttccacaata aaaatatctt
    34201 gatctcggac gaaatccagt gcttcttgaa aaacaagtcg atgagtaatc gttgcgccag
    34261 attgtttctc aatgaaaatt ctctctgcac cgaatttttt catttcttca atctgccgtt
    34321 gttcattttg atcgatggat gagacacgta tgtatgcaac tctcaaaatt tttacctact
    34381 ttctgcaact atttaaaaaa caccctaatc atacacgaga aaaccagatt tttcaatgtc
    34441 gcggtcgtgt ctagttaggg tataccttat atagacaaaa actaatgatg gaggaaatag
    34501 tatgtgtcac ttttcttttt aaatggcaat attttttgta aataaatgtc aatcaagaaa
    34561 atttaaacga ttatgacatt aaaaatatat tttatgaatt ttctagtata taacttgatt
    34621 tatctgttaa gatttattaa gaaaacgttt tcttattttt gatttcgagg tgatagacaa
    34681 tgaaaaataa gtttatttta tctttaggta ttttaactat tggaattagt gtaactggaa
    34741 ctacaattcc tacatttgct gatagtacaa atgaaccaac tactgtagta gataataacc
    34801 aagaattaac tcctttagat aggaacggaa tagccattgg taacccaact aacgacttaa
    34861 ctgttactga aaattattta atagtttctc cattacttgc tcgagcagct tctaaaacga
    34921 tacgagtgac aggaacgatg atttttgata aagttggttg gccttctaga cctcctcaat
    34981 cacggaattg gaaagaaaaa agaaatggtt attggtacta tggaaaattg tatttacaaa
    35041 gttataaatc agctggaaac aattggattg cgacttattc aggaacctta actagaaagt
    35101 agggattttt tatgccagaa tttgtgaaca caccaaattt ttatcaagat agcgatattc
    35161 taagtaaaat taatttaccg aaagaagaat cagctttaca agttcagcga actttacagg
    35221 agacacaaaa aattcaaaaa tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa
    35281 aaagagacca aaaaacatat caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt
    35341 cgacattctt tttttctaat tgaaagtgtt cataaaatca cccatttctg gacactttct
    35401 ttttttgctt tatttttcaa aaaaacaatt catttcatgt tcataaaccc ataacaatat
    35461 ctatgttcag ttaaactata tggataaagt tatgttacaa taaaattaat gaaggagtga
    35521 tttttttgaa aataggatac gcccgggtat ctactggttt acaaaatttg gatttacaag
    35581 aagacagcct aaataaattt ggttgtgaaa aaatttttac tgatcatatg agtggggcaa
    35641 aaagtaatcg accaggatta gaaatggcca ttgattttgt tcgctctggc gatacacttg
    35701 tcgtttggcg cttagatcgt ttaggtagaa atatggaaga tttgatttca atcgtcaatc
    35761 gactgaatga gcgtggtgtg agttttcata gtattcaaga aaatattacg atggacaaat
    35821 caagttctac aggacagctc atgtttcatt tgtttgcggc atttgcagaa tttgagcgta
    35881 atttgatcct ggaacgatcc gcagcaggac gagcagctgc tcgtgcaaga ggacggtttg
    35941 gtggtcgtcc tgaaaaattg tctgaacaag atttggaatt actgaagact ttagtggata
    36001 gcggtacacc tattaagact attgcggaac ggtggaacgt ctcaagaacc accatttatc
    36061 gttatttggc aaaaatggag gagaaaaatg gaaagacaaa ttctcattag tttttgtcat
    36121 ttaatgctga aaatttttgc catttatcta gaaaagtgac aatagtatga gaattttttg
    36181 taagtgtcta ctgataactt attctcaaat gttgtaagga ttttattcat ttgttaatat
    36241 taa
//
LOCUS       DAZKCE010000014        34545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000014 DAZKCE010000000
VERSION     DAZKCE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.014"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..2144)
                     /locus_tag="ACG8KQ_000636"
     CDS             complement(252..2144)
                     /locus_tag="ACG8KQ_000636"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103604.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2141..3868)
                     /locus_tag="ACG8KQ_000637"
     CDS             complement(2141..3868)
                     /locus_tag="ACG8KQ_000637"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103605.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3884..4534)
                     /locus_tag="ACG8KQ_000638"
     CDS             complement(3884..4534)
                     /locus_tag="ACG8KQ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9103606.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4738..5034
                     /locus_tag="ACG8KQ_000639"
     CDS             4738..5034
                     /locus_tag="ACG8KQ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9103607.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5206..5826
                     /locus_tag="ACG8KQ_000640"
     CDS             5206..5826
                     /locus_tag="ACG8KQ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9103608.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5918..7084)
                     /locus_tag="ACG8KQ_000641"
     CDS             complement(5918..7084)
                     /locus_tag="ACG8KQ_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9103609.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7096..7449)
                     /locus_tag="ACG8KQ_000642"
     CDS             complement(7096..7449)
                     /locus_tag="ACG8KQ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9103610.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7577..8599
                     /locus_tag="ACG8KQ_000643"
     CDS             7577..8599
                     /locus_tag="ACG8KQ_000643"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9103611.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8618..8779
                     /locus_tag="ACG8KQ_000644"
     CDS             8618..8779
                     /locus_tag="ACG8KQ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103612.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9038..9472)
                     /locus_tag="ACG8KQ_000645"
     CDS             complement(9038..9472)
                     /locus_tag="ACG8KQ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103613.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9582..11441)
                     /locus_tag="ACG8KQ_000646"
     CDS             complement(9582..11441)
                     /locus_tag="ACG8KQ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9103614.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11442..11582
                     /locus_tag="ACG8KQ_000647"
     CDS             11442..11582
                     /locus_tag="ACG8KQ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9103615.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11559..12719)
                     /locus_tag="ACG8KQ_000648"
     CDS             complement(11559..12719)
                     /locus_tag="ACG8KQ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9103616.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13026..14450)
                     /gene="araA"
                     /locus_tag="ACG8KQ_000649"
     CDS             complement(13026..14450)
                     /gene="araA"
                     /locus_tag="ACG8KQ_000649"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9103617.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14466..15164)
                     /locus_tag="ACG8KQ_000650"
     CDS             complement(14466..15164)
                     /locus_tag="ACG8KQ_000650"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9103618.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15121..16782)
                     /locus_tag="ACG8KQ_000651"
     CDS             complement(15121..16782)
                     /locus_tag="ACG8KQ_000651"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9103619.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17000..18400
                     /locus_tag="ACG8KQ_000652"
     CDS             17000..18400
                     /locus_tag="ACG8KQ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9103620.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18652..19731)
                     /locus_tag="ACG8KQ_000653"
     CDS             complement(18652..19731)
                     /locus_tag="ACG8KQ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9103621.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19817..20776)
                     /locus_tag="ACG8KQ_000654"
     CDS             complement(19817..20776)
                     /locus_tag="ACG8KQ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9103622.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20788..22299)
                     /locus_tag="ACG8KQ_000655"
     CDS             complement(20788..22299)
                     /locus_tag="ACG8KQ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9103623.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22321..22884)
                     /locus_tag="ACG8KQ_000656"
     CDS             complement(22321..22884)
                     /locus_tag="ACG8KQ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9103624.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22890..23753)
                     /locus_tag="ACG8KQ_000657"
     CDS             complement(22890..23753)
                     /locus_tag="ACG8KQ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103625.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23767..24663)
                     /locus_tag="ACG8KQ_000658"
     CDS             complement(23767..24663)
                     /locus_tag="ACG8KQ_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103626.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24838..26127)
                     /locus_tag="ACG8KQ_000659"
     CDS             complement(24838..26127)
                     /locus_tag="ACG8KQ_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9103627.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26099..27616)
                     /locus_tag="ACG8KQ_000660"
     CDS             complement(26099..27616)
                     /locus_tag="ACG8KQ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9103628.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27814..28704
                     /locus_tag="ACG8KQ_000661"
     CDS             27814..28704
                     /locus_tag="ACG8KQ_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9103629.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28777..29601)
                     /gene="nadE"
                     /locus_tag="ACG8KQ_000662"
     CDS             complement(28777..29601)
                     /gene="nadE"
                     /locus_tag="ACG8KQ_000662"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9103630.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29613..31085)
                     /locus_tag="ACG8KQ_000663"
     CDS             complement(29613..31085)
                     /locus_tag="ACG8KQ_000663"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9103631.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31722..32084)
                     /locus_tag="ACG8KQ_000664"
     CDS             complement(31722..32084)
                     /locus_tag="ACG8KQ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9103632.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32307..33059
                     /locus_tag="ACG8KQ_000665"
     CDS             32307..33059
                     /locus_tag="ACG8KQ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9103633.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33199..34122)
                     /locus_tag="ACG8KQ_000666"
     CDS             complement(33199..34122)
                     /locus_tag="ACG8KQ_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103634.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34137..34409)
                     /locus_tag="ACG8KQ_000667"
     CDS             complement(34137..34409)
                     /locus_tag="ACG8KQ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103635.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10190 a         7201 c         5949 g        11205 t
ORIGIN      
        1 ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
       61 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat
      121 agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt
      181 ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa
      241 tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag
      301 aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc
      361 atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt
      421 gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct
      481 acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca
      541 attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg
      601 ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca
      661 atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct
      721 gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa
      781 agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt
      841 tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc
      901 tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg
      961 aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat
     1021 ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact
     1081 gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt
     1141 gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact
     1201 ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg
     1261 atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg attttctttt
     1321 tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca
     1381 tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac
     1441 acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac
     1501 gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg
     1561 actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata
     1621 tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt
     1681 acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca
     1741 cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt
     1801 actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg
     1861 ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc
     1921 cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca
     1981 gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag
     2041 aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga
     2101 ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc
     2161 ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg
     2221 ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg
     2281 tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt
     2341 ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga
     2401 tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga
     2461 agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat
     2521 ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac
     2581 catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc
     2641 ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga
     2701 tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg
     2761 aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt
     2821 ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg
     2881 cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt
     2941 catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa
     3001 tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca
     3061 gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta
     3121 cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa
     3181 ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc
     3241 gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt
     3301 ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc
     3361 cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat
     3421 ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac
     3481 gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca
     3541 aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga
     3601 catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg
     3661 cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa
     3721 tataatcgac atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca
     3781 aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca
     3841 aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt
     3901 ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt
     3961 cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta
     4021 attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca
     4081 attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt
     4141 ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt
     4201 tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg
     4261 attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag
     4321 agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat
     4381 actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt
     4441 cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt
     4501 ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat cctcctttct ttcaagatga
     4561 cacattgtca tatgacgctg tgtcattata acacctccta cctgttttgc aagacatttg
     4621 catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt
     4681 tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg
     4741 gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt
     4801 ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc
     4861 cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt
     4921 ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt
     4981 cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg
     5041 cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg
     5101 tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt
     5161 ctcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta
     5221 gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct
     5281 tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat
     5341 cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct
     5401 ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa
     5461 ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta
     5521 aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc
     5581 cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa
     5641 tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta
     5701 aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct
     5761 gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca
     5821 ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca
     5881 tttgtacctt ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg
     5941 gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta
     6001 ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc
     6061 cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta
     6121 ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt
     6181 tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa
     6241 atgatcctaa gtaagggatc aggttcaaaa aacccgcaat aatccccaac gtgacagcat
     6301 actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag
     6361 ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg
     6421 tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt
     6481 ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta
     6541 ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat
     6601 cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga
     6661 acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga
     6721 agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc
     6781 atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt
     6841 ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa
     6901 tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt
     6961 ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt
     7021 gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt
     7081 ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct
     7141 atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat
     7201 caatgatacg tgagctactg tctctaaatt cccattggcc atcggaccga cataatcgat
     7261 cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc
     7321 ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa
     7381 caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc
     7441 ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc
     7501 tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta
     7561 ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag
     7621 taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt
     7681 agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac
     7741 agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt
     7801 gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca
     7861 attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga
     7921 tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa
     7981 ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg
     8041 tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct
     8101 tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa
     8161 tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa
     8221 taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga
     8281 cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc
     8341 taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca
     8401 aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag
     8461 caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca
     8521 tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg
     8581 tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga
     8641 cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta
     8701 tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt
     8761 acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac
     8821 ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc
     8881 gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt
     8941 atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt
     9001 agataactct agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag
     9061 tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg
     9121 tattgaatag atcttcatct acccctgatt caagatcgat actttggatc atttcttcac
     9181 agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg
     9241 ctggtggacg caaagtactg actttcttaa gcattgcccg atattttttt gttccttctt
     9301 caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat
     9361 caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt
     9421 ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt
     9481 tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag
     9541 tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa
     9601 ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg
     9661 tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga
     9721 tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa
     9781 aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg
     9841 gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat
     9901 tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct
     9961 ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc
    10021 gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca
    10081 agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg
    10141 tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg
    10201 agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc
    10261 gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa
    10321 acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat
    10381 tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg
    10441 taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat
    10501 tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga
    10561 tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga
    10621 ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca
    10681 gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct
    10741 ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg
    10801 ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta
    10861 ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt
    10921 ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg
    10981 cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact
    11041 gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa
    11101 tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat
    11161 acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc
    11221 agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa
    11281 gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat
    11341 aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc
    11401 tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc
    11461 tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac
    11521 ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt
    11581 aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc
    11641 tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta
    11701 cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat
    11761 atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt
    11821 gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct
    11881 tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc
    11941 aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta
    12001 gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt
    12061 cctacattga taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat
    12121 ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact
    12181 gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag
    12241 acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca
    12301 agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct
    12361 gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc
    12421 ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca
    12481 cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc
    12541 accccaacac cagctcttga aattgctagc aaatctggtg taagccattc atctttcact
    12601 ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc
    12661 ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata
    12721 aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat
    12781 ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag
    12841 gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata
    12901 tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt
    12961 gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc
    13021 tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga
    13081 tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa
    13141 tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta
    13201 ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta
    13261 ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc
    13321 gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca
    13381 gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct
    13441 agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc
    13501 aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc
    13561 atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta
    13621 gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga
    13681 gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta
    13741 tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt
    13801 acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct
    13861 tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact
    13921 tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt
    13981 tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca
    14041 ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata
    14101 tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt
    14161 tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg
    14221 atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca
    14281 gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt
    14341 tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa
    14401 tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac
    14461 tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta
    14521 tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc
    14581 atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc
    14641 caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg
    14701 cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt
    14761 tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc
    14821 gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga
    14881 gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta
    14941 tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg
    15001 tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt
    15061 tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag
    15121 tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt
    15181 ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat
    15241 aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg
    15301 tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata
    15361 ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac
    15421 tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc
    15481 cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat ccattccgat
    15541 tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg
    15601 gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc
    15661 ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt
    15721 gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc
    15781 ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc
    15841 tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc
    15901 tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtccgttt
    15961 ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg
    16021 gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt
    16081 gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt caatcgctac
    16141 gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt
    16201 tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt
    16261 aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc
    16321 ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta
    16381 ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg
    16441 aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact
    16501 gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc
    16561 catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat
    16621 accattttct agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga
    16681 atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc
    16741 atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc
    16801 ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact
    16861 tttgtacgta catgtcaagc ggttatttac aagaatacaa taaaaaacac ttgttttgta
    16921 cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt
    16981 ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt
    17041 ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt
    17101 acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac
    17161 gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa
    17221 gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct
    17281 ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg
    17341 tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc
    17401 tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct
    17461 ttcctatgtt gcagattatt tgctaaaagt gttgcctgaa acgatggctt ggcgcgtgat
    17521 gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga
    17581 gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta
    17641 tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca
    17701 agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt
    17761 cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta
    17821 ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg
    17881 gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga
    17941 caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttcattcat
    18001 cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt
    18061 gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat
    18121 cataggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt
    18181 taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc
    18241 tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca
    18301 agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa
    18361 aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg
    18421 tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa
    18481 aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt
    18541 ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa
    18601 cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc
    18661 gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc
    18721 ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt
    18781 tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt
    18841 tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat
    18901 taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa
    18961 ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc
    19021 ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta
    19081 ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat
    19141 caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt
    19201 taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac
    19261 ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc
    19321 taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt
    19381 actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat
    19441 cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt
    19501 caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt
    19561 acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac
    19621 gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc
    19681 acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc
    19741 ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa
    19801 tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt
    19861 gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc
    19921 ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg
    19981 aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa gaaacgaata
    20041 ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc
    20101 acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt
    20161 tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt
    20221 catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt
    20281 cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat
    20341 atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt
    20401 ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta
    20461 cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt
    20521 aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg
    20581 gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg
    20641 actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat
    20701 aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat
    20761 aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta cattccaaga
    20821 gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc
    20881 aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa
    20941 tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg
    21001 ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact
    21061 gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg
    21121 tttcaatact tgacctttag catatttcga taagtgttgc atcacataat aagttggatt
    21181 cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga
    21241 ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa
    21301 taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc
    21361 cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt
    21421 tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg
    21481 acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg
    21541 aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc
    21601 tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg
    21661 tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt
    21721 tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg
    21781 accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt
    21841 ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt
    21901 ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac
    21961 atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca
    22021 tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata
    22081 accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata
    22141 agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat
    22201 ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat
    22261 ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc
    22321 ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa
    22381 gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca
    22441 acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg
    22501 taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga
    22561 ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa
    22621 ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa
    22681 atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca
    22741 ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca
    22801 cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata
    22861 aaatttagtc tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct
    22921 tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc
    22981 gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct
    23041 gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc
    23101 caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac
    23161 atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa
    23221 gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata
    23281 cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat
    23341 agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa
    23401 actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat
    23461 acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa
    23521 gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta
    23581 ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa
    23641 ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca
    23701 ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt
    23761 tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat
    23821 agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg
    23881 tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc
    23941 accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa
    24001 aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg
    24061 atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc
    24121 agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt
    24181 cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa
    24241 caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga
    24301 cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt
    24361 atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag
    24421 tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg
    24481 tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa
    24541 actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga
    24601 tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt
    24661 catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct
    24721 gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag
    24781 gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta
    24841 tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc
    24901 tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg
    24961 ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat
    25021 aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt
    25081 ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac
    25141 agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat
    25201 agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc
    25261 gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc
    25321 attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg
    25381 gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt
    25441 agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc
    25501 agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg
    25561 tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg
    25621 tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata
    25681 ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac
    25741 tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc
    25801 aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg
    25861 aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc
    25921 atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa
    25981 cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc
    26041 accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt
    26101 acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata
    26161 gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg
    26221 gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag
    26281 aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt
    26341 cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt
    26401 ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc
    26461 ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc
    26521 tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacgtttacc aattgagcaa
    26581 gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata
    26641 gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg
    26701 ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt
    26761 tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag
    26821 aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt
    26881 tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg
    26941 ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct
    27001 caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt
    27061 cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg
    27121 tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc
    27181 ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca
    27241 gattgacagc catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa
    27301 tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg
    27361 ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc
    27421 taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt
    27481 tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt
    27541 caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca
    27601 acattgtggc ttccataact gacctccttt aatttataca tgaataatac acctcgttga
    27661 aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt taattaacca
    27721 aaatattaat aatgattaaa acaaataatt taatataact tttattaaca aatatgttaa
    27781 tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa cgaagaatcc
    27841 ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca gctactttcc
    27901 aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag tgctatcacg
    27961 acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa agtaggacaa
    28021 caaaaaattt cgagccttcg tgtcgataaa atcgatatta gttttccaga aaaaatattc
    28081 aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa ttatgcaatt
    28141 gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga tgaaccacgc
    28201 tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag cggatttgtc
    28261 gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt ggaagtttct
    28321 gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga tatcactttc
    28381 agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc tgatatccgc
    28441 ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt gctgaagacg
    28501 atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa catcacgatc
    28561 aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa agacgatgcc
    28621 aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga tcaggatatc
    28681 aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac aaaaacagcc
    28741 aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat catccaaaac
    28801 ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga ttttttcttg
    28861 tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat atgtgacacc
    28921 tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag tcggaatttt
    28981 tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc
    29041 caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga agaatccagt
    29101 aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc cagctacggc
    29161 atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat cgctgattcc
    29221 cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga tatttactcg
    29281 cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt catctgcttg
    29341 ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg tttcttcacg
    29401 catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct
    29461 gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt tcataaagtc
    29521 aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta attcattgat
    29581 aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag ttctgctacg
    29641 ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt agacagatcg
    29701 actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga atctagattt
    29761 tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg
    29821 ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt attgataaac
    29881 gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc atcccataat
    29941 gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca cacttgtttt
    30001 ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc catcattttt
    30061 ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg ctgatcatag
    30121 gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg catctttaaa
    30181 ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc cgtaaatccg
    30241 gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc actgtcgatg
    30301 cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat cgcacttggc
    30361 acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt atgggtttta
    30421 gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga atgagcatgg
    30481 gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt tgcatctgct
    30541 ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg tgcccgtcta
    30601 gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc tgcttttgtc
    30661 gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa ttggcagtgc
    30721 gctaaaggcc cttccacttg gatcaatggt tcatttgcaa aaactagttc tccttcacga
    30781 gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa accttcagga
    30841 tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag attctctaaa
    30901 taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct
    30961 cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca gtatgtctgc
    31021 atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta
    31081 ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata acaaaaaaaa
    31141 ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac ggatatttct
    31201 tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt aacctaaacg
    31261 agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc gttttctatt
    31321 tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga
    31381 tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag
    31441 caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat
    31501 gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc
    31561 gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga
    31621 atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt gtatacatta
    31681 gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga atccttcgta
    31741 tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat tccggcacgt
    31801 tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat ttttaaaacg
    31861 tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg
    31921 acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa cgaaaataat
    31981 ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg ataaaatgtc
    32041 ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt cctcttttct
    32101 atatagtcga tttagatcca atcttaagtt atgttaccat ctttcctagc attaaggaat
    32161 cggttacaat tgaaccttta aagacaaaag gatttacctt ttatcactta atagtataac
    32221 ataatttcat gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt aaaggattat
    32281 gcttacattt gtcaggagga aaaataatgg agtacacaat aaagaaaatg tcagagatat
    32341 caggagtcag tccgagaacc ttgcgctttt atgatgagat tggactgcta aaaccagcta
    32401 ggatcaattc ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca
    32461 tcttatttta tcgaacaatg gcattcaagt tggatgatat ccaagaggtc ttagacaatc
    32521 catcttttga ccaccagaaa gctttaatca agcatcagca aatgttgctg gaaaaacggg
    32581 cacaaatcga taccctcttg acaactgtcc aacagacact tgatatgtac gaaggaggaa
    32641 gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg
    32701 aagaatccta tggacaagag attcgacaaa aatacggaaa aaaaaaggtt acagcatcca
    32761 atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc
    32821 agatgttatt tgatctcact ctttacttaa aagaaccaga agatgaaagc ttagcagacc
    32881 gtatcttcca agctcataag aagtggttat cttacagctg gcctgaatat cagccaaaag
    32941 cgcataaagg actaggaatg atgtacgaag ccgatgaacg gttcactgca tattatgacg
    33001 agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat
    33061 cttttacatg atccaacaaa aaagtacacg taaaatgacc taataacaaa acagcagaca
    33121 cccaagttag atttttatgc taacttttgg tgtctgctcc attctttcac aagttttttc
    33181 atcctacttt aaatatattc atcgttgaat aaacgtataa ggtattttca ctttatcctt
    33241 tttttctgta agaaacaatt gaaatgccat ttctccgcaa gcttccagat ggtggtcgat
    33301 ggttgggaaa ccaagaagct cgctgatcag caaattatcg cgtccaatca ccactggtgt
    33361 cttatcttgc agatagcttt gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac
    33421 accatcaact ccaagctctt taaaaaagaa gcctgcttgt tttcctttct ctgcatctat
    33481 acaatcccaa aaaacaagtg attcatcaaa agatggaaaa agttgatgac atagctgcaa
    33541 agtgattttt gagttataac ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt
    33601 tataccttgc tgcttcaaat aagtaagacc ttctgtaatt gattgttctc gatcaataaa
    33661 gacacagctt gcctgttttc ccttgatttc ttcacaaaaa actagaggtc catatttttg
    33721 atatgccaat aaatcatcta gtgtattggc acgagacgta atgatcaatc catcgaaagc
    33781 tttagctgca aattcttcaa gataccttcg ctctttttct ttctgatagc ccgtcggtaa
    33841 aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg atcccactga tcaactgatc
    33901 aaaatacgga tgattggtat aaggcagaat cactccaatg cttttcgttt ttccataact
    33961 taactgtctg gcatttgcat tagggatata atcgacttct ttcatgaccg caagtatttc
    34021 tttccttttt tcctcatcta catatggatg tccattgatt acacgtgaaa tagtagaaac
    34081 tgaataacct gtgagctttg cgatatctcg aatgtttgcc actgctgctt cgccacctat
    34141 acaaaaagat tttcttgtaa atagtctacc acagctggac cgcattcaaa aaactgatac
    34201 atttttcctt catgcgaaaa actgatccgc tgcccgcccc aatatatatc ttttgtcaaa
    34261 ttttcgattt cattcattgg tatttctttt actatattcc tattcaaccc ttgaaaactg
    34321 tacaacgtta atacggatcc cttgaactcg acaaacgcat aaaaataatt gttcgttgtg
    34381 ccatctgtta tctctacata agtattcatt gcaagccctc cttttgatac tttgagtata
    34441 gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt tttattcctc
    34501 tttcattatt accaaagtct cttattttaa attgaataca ttata
//
LOCUS       DAZKCE010000015        34365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000015 DAZKCE010000000
VERSION     DAZKCE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.015"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            269..958
                     /locus_tag="ACG8KQ_000668"
     CDS             269..958
                     /locus_tag="ACG8KQ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9103636.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            945..2153
                     /locus_tag="ACG8KQ_000669"
     CDS             945..2153
                     /locus_tag="ACG8KQ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9103637.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2447..3718
                     /gene="serS"
                     /locus_tag="ACG8KQ_000670"
     CDS             2447..3718
                     /gene="serS"
                     /locus_tag="ACG8KQ_000670"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9103638.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4267..5751)
                     /gene="guaB"
                     /locus_tag="ACG8KQ_000671"
     CDS             complement(4267..5751)
                     /gene="guaB"
                     /locus_tag="ACG8KQ_000671"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9103639.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6256..6951)
                     /locus_tag="ACG8KQ_000672"
     CDS             complement(6256..6951)
                     /locus_tag="ACG8KQ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9103640.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7041..8141)
                     /gene="ychF"
                     /locus_tag="ACG8KQ_000673"
     CDS             complement(7041..8141)
                     /gene="ychF"
                     /locus_tag="ACG8KQ_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9103641.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8150..8341)
                     /locus_tag="ACG8KQ_000674"
     CDS             complement(8150..8341)
                     /locus_tag="ACG8KQ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9103642.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8362..9252)
                     /locus_tag="ACG8KQ_000675"
     CDS             complement(8362..9252)
                     /locus_tag="ACG8KQ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9103643.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9239..10006)
                     /locus_tag="ACG8KQ_000676"
     CDS             complement(9239..10006)
                     /locus_tag="ACG8KQ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9103644.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10018..10731)
                     /gene="rsmG"
                     /locus_tag="ACG8KQ_000677"
     CDS             complement(10018..10731)
                     /gene="rsmG"
                     /locus_tag="ACG8KQ_000677"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9103645.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10963..12864)
                     /gene="mnmG"
                     /locus_tag="ACG8KQ_000678"
     CDS             complement(10963..12864)
                     /gene="mnmG"
                     /locus_tag="ACG8KQ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9103646.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12881..14278)
                     /gene="mnmE"
                     /locus_tag="ACG8KQ_000679"
     CDS             complement(12881..14278)
                     /gene="mnmE"
                     /locus_tag="ACG8KQ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9103647.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14577..14960
                     /locus_tag="ACG8KQ_000680"
     CDS             14577..14960
                     /locus_tag="ACG8KQ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103648.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15128..15886)
                     /gene="jag"
                     /locus_tag="ACG8KQ_000681"
     CDS             complement(15128..15886)
                     /gene="jag"
                     /locus_tag="ACG8KQ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9103649.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15904..16722)
                     /locus_tag="ACG8KQ_000682"
     CDS             complement(15904..16722)
                     /locus_tag="ACG8KQ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9103650.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16719..17075)
                     /gene="rnpA"
                     /locus_tag="ACG8KQ_000683"
     CDS             complement(16719..17075)
                     /gene="rnpA"
                     /locus_tag="ACG8KQ_000683"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9103651.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17193..17327)
                     /gene="rpmH"
                     /locus_tag="ACG8KQ_000684"
     CDS             complement(17193..17327)
                     /gene="rpmH"
                     /locus_tag="ACG8KQ_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9103652.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17850..19184
                     /gene="dnaA"
                     /locus_tag="ACG8KQ_000685"
     CDS             17850..19184
                     /gene="dnaA"
                     /locus_tag="ACG8KQ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9103653.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19382..20512
                     /gene="dnaN"
                     /locus_tag="ACG8KQ_000686"
     CDS             19382..20512
                     /gene="dnaN"
                     /locus_tag="ACG8KQ_000686"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9103654.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20741..20983
                     /gene="yaaA"
                     /locus_tag="ACG8KQ_000687"
     CDS             20741..20983
                     /gene="yaaA"
                     /locus_tag="ACG8KQ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9103655.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20970..22094
                     /gene="recF"
                     /locus_tag="ACG8KQ_000688"
     CDS             20970..22094
                     /gene="recF"
                     /locus_tag="ACG8KQ_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9103656.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22091..24037
                     /gene="gyrB"
                     /locus_tag="ACG8KQ_000689"
     CDS             22091..24037
                     /gene="gyrB"
                     /locus_tag="ACG8KQ_000689"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9103657.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24057..26528
                     /gene="gyrA"
                     /locus_tag="ACG8KQ_000690"
     CDS             24057..26528
                     /gene="gyrA"
                     /locus_tag="ACG8KQ_000690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9103658.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26764..27063
                     /gene="rpsF"
                     /locus_tag="ACG8KQ_000691"
     CDS             26764..27063
                     /gene="rpsF"
                     /locus_tag="ACG8KQ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9103659.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27113..27625
                     /gene="ssb"
                     /locus_tag="ACG8KQ_000692"
     CDS             27113..27625
                     /gene="ssb"
                     /locus_tag="ACG8KQ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9103660.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27651..27887
                     /gene="rpsR"
                     /locus_tag="ACG8KQ_000693"
     CDS             27651..27887
                     /gene="rpsR"
                     /locus_tag="ACG8KQ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9103661.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28030..30003
                     /locus_tag="ACG8KQ_000694"
     CDS             28030..30003
                     /locus_tag="ACG8KQ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP9103662.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30009..30461
                     /gene="rplI"
                     /locus_tag="ACG8KQ_000695"
     CDS             30009..30461
                     /gene="rplI"
                     /locus_tag="ACG8KQ_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9103663.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30735..32102
                     /gene="dnaB"
                     /locus_tag="ACG8KQ_000696"
     CDS             30735..32102
                     /gene="dnaB"
                     /locus_tag="ACG8KQ_000696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9103664.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32882..34174
                     /locus_tag="ACG8KQ_000697"
     CDS             32882..34174
                     /locus_tag="ACG8KQ_000697"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9103665.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10480 a         6691 c         6815 g        10379 t
ORIGIN      
        1 catgtgaaag tcaataagaa aatgcttatt tatcaacatt tctagcttac atgatcattg
       61 gttagttctg gtcctttttc gttcgtcttt ataactccga gagacgtaaa atccttttgt
      121 tttcagttta atgttcatgt tcttgtctta ttcacaccaa ctagatgtct caccttctaa
      181 aattttcctt aacaaagtgc tgcttttatc tatttctgct tttttttgct agaataatag
      241 taacaatatg actctggagg atttatttat gaaaattttg attgctgacg atgataaaga
      301 aatcgttgaa ttattaagta tctatgttca caatgagggg tatgaagcag taaaggctta
      361 tgatgggaaa gaagcattat ctaaaatcag aacaatccct gatattgaat tacttatttt
      421 agatatcatg atgcctgaga tggatgggat gcaagtagtg aaagagctac gaaaagaatc
      481 ccaaatacca atcattatgc tgacagcaaa aactactgat atggataaga tcaaaggact
      541 ggtcgctggt gcagatgatt atgtgaccaa gccatttaat ccgttagaag taatggctag
      601 agtaaagtct ttattacggc gtacaaaaat gcagattgcc ccagatcagc ctgatgtatt
      661 agaagtaaat gcattaatca tcaataaaga ctctcatgaa gttaaaacaa tcgatgggaa
      721 agaaatccag ttaacagctt tggaattcgg tatcctctac ttgcttgcta gtcatcctaa
      781 tcgagtattt agcgcggatg aaatttttga gcgtgtttgg aaacaagaaa gtattgtctc
      841 tgctaaaact gtcatggttc acgttagtca tttaagagat aagattgaag aagcaactgg
      901 tggtgaaaaa gtcattcaaa ccgtttgggg agtgggctat aaaattgacg caagatagta
      961 aacgagcaac tgaaaaacgc cgtcggatca atttgacttc aaaagaaatc agcgaattgt
     1021 tagcggaagg aatcatcacg attatccttt tgcttctatt aaatgtttct attctggttg
     1081 ttatcagttc ggtgatcaac agttcacctt ctttgacgaa tgcgatttgg gattcaaaga
     1141 atatttttgc agaacgattg aacactgatt tgttctggaa cggccggaat tttattattc
     1201 cctttttctt tctattagat attggtgttt tgtattggcg cttgattcga agatatcgcc
     1261 aaatgcaatt gcggcatatc atcagcgaac ttcactacat cgcaaacgga aattatgatc
     1321 atcgtatccc ttttgaactg agtggtgact taagccgagt ggtaacaagt atcaatggat
     1381 tggtggatag taccgtagca gcgattgaag acgaacgtaa aatcgaaaaa tcaaaagatg
     1441 aattgatcac gaatgtcagt catgatatcc gtaccccact cacgtccatc attggatacc
     1501 taggactgat agaagatggc cagtatcact cagaagaaga tcttttaaag tatacgcata
     1561 ctgcttatat caaagcaaag cagatgaaat ctttagtgga cgacttattt gaatacacta
     1621 aggtacgcca gcctgctgtc ccggtgaatt tttctgcctt tgacatgatc cagttgatcg
     1681 aacagttagc agctgatttt gagttggaag cttctaaaaa aaatatccaa atacttgttc
     1741 aatcaaaagt agactccctt atcatggatg gcgataccga aaaacttgta cgtgtcttca
     1801 ataatctttt gacgaatgcg ttgaaatacg gaaaaggagc aacgaagatc gtaatcgaag
     1861 tagaacgtat cggttcagaa gttgttgcaa cagtcaaaaa taacggagca atgatcccac
     1921 aacaggcaat cgataatcta ttcgaccgtt tttatcgtgt agaagaatct cgttcacaag
     1981 caactggagg aactggactt ggtttagcaa tcgctcaaag cattgtggct ttgcacggcg
     2041 gatacattta tgcaaaatca gataagcaat ggacttcttt catcatgcac cttccaatca
     2101 agaaaaatga aaaattacct attgatacac aggaagttat tgacgaaagc tgaaatattc
     2161 gctattctta ttacaattat tagaataaat gaaaaacaca gaacccagta cactttattc
     2221 tttttcagag agctgatggc aggtgtaaat cagcaaagga taatttggaa gttccagttc
     2281 gtttacgtgc cctttcgaaa taagaaaggt cggttcaaca gccgttatgt gttcaacaag
     2341 tgcagctgac gctgaacttg ggtggtaccg cgaatttcag actgattcgt cccaagaata
     2401 atgggatgga tcagtctttt tttattttca aaaaggagga aatcatatgt tagatgtaaa
     2461 aatgatgcga caaaatattg aagaagtaca agaaaaattg atgactcggg gcgtaaaaaa
     2521 agaaacatta gctgaattca tggaactaga tgaaaatcgt cgtaagctac tagtcaaaag
     2581 tgaagaatta aaaaaatatc gaaatgatgt ttctgctgag atcgcccaat tgaagcgtgc
     2641 aaaagaagat gcttctgcta aaatcgctga aatgaaagaa gtcggtggca acatcaaagc
     2701 attggatact gaaattgctg aagtggatga aaaattgaaa caaattgcaa ctactttgcc
     2761 aaaccttccc catgattctg tgccagttgg aacagatgaa gaggacaatg tagaaatccg
     2821 tcgttggagc gaaccacgta catttgcttt tgaaccaaaa ccacactggg aaattgcaga
     2881 aaatcttgat attttagatt ttgaacgtgg cgcaaaagta gccggtagcc gttttgtcta
     2941 ttacaaagga ttaggtgctc gattagaacg tgctgtctac aacttcatgc ttgatcagca
     3001 tatttacgaa cacggctata ctgaaatgat cactccttat atcgtcaaca gtaaagcgat
     3061 gtttggtacg ggacaatttc caaaattcaa agaagatgtt ttccaattgg agaatactga
     3121 tctaacatta atcccaacag ctgaagtacc attaactaac tattataatg atgaaatttt
     3181 agatggtgca gatttaccta tttatttcac tgcactaagc cctgcttttc gttctgaagc
     3241 cggaagtgct ggtcgggata cacgaggact gattcgtctt caccaattca ataaagtaga
     3301 aatggtcaaa ttttcggatg tagagcattc ttacgaagaa ttggaaaaaa tgacagctga
     3361 tgcagaagat atcttgaaaa aattgcattt gccttatcgt gtgatggcct tatcaactgg
     3421 agacatgggc ttttctgcag ctaaaactta tgacttagaa gtatggatac ctgcacaaga
     3481 tacttatcgt gaaatcagtt cttgttctaa ctgtgaagat ttccaagcac gccgcgcaat
     3541 gatccgttat cgcgataatg atggaaaaat ccactatact catactctga atggttcagg
     3601 acttgcagtt ggccgtacag tagctgccat tttggaaaac taccaaaatg aagatggttc
     3661 tgtcacagtt ccagaagcgc tcgttcctta tatggggaat ctgaaagtta ttaaataaag
     3721 aggctgtgaa aaagccgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc
     3781 cacatttttg cataaattgg acttaatacc gagaaactgg cttttgaaca ccatttatcg
     3841 agaggttgtg aaatcagcga attaacctga gtattagaga aaaaaatgga aaaatagtcc
     3901 ctcatatttt accgtttgtt gaactaatac cgagggtcag ctgattgaac accgtttagt
     3961 ggagaggtcg taagcctacc ccccagctcc tagttacaag gacaaattca aaaaacagct
     4021 cttcatattt tatgaatgtt gtacttgtga ttcggaggtg gcaagggaac actgtttatt
     4081 caaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat tcacgaaaat
     4141 tgcttttcaa atttttgtga atttcttctt atttccgaag gagttgcttc tacttccgcc
     4201 gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta
     4261 atgtttttaa gattcaactg aatagtttgg tgcttctttt gtgatttgta catcatgagg
     4321 gtgtgattct tttaatccac tgccgctcat ttggataaat tgcgcttcat cacgtaattg
     4381 ctgtagattg gctgccccaa cataacccat tcctgaacgc aaaccgccga tcatttggaa
     4441 aataatatct gcaacacttc ctttataagc aacccgacct tcgattcctt ctggtactaa
     4501 tttatttgct tcattgacac taccttggaa gtaacggtct tttgaacctt tttccatcgc
     4561 gcctaatgag cccatacctc ggtatgtttt gaatcgacgt ccttggaaga tttcgaattc
     4621 acctggtgat tcatctgtcc ctgccaacat acttcctagc atcactgcat gtccgccggc
     4681 agctaatgct ttgacgatgt caccagaata tttgatccct ccgtctgcaa taatcgcttt
     4741 gccatattga cgagcaactg atgctgcatc ataaatagct gtgagctgag gtacacctac
     4801 accggctacc acacgtgtcg tacaaatcga tcctggtccg ataccaactt ttacgacatc
     4861 tacacctgca tcatataaag cttttgtcgc ttcagccgtc gcaacgtttc ctgcaatcaa
     4921 tgttgcatct gcaaaagttg aacggatttc ttgaattttt cggatcacac ctgcactatg
     4981 tccatgagct gtatcgatga tgattgcatc tgcccctgct tctaataaag cattcgcacg
     5041 ctcaaatgta tcgcttgtta caccaacggc tgctgctaca agcaatcgac catgttcatc
     5101 ttttgctgca tttggaaatt cgatcacttt ctcgatgtct ttgatcgtga tcaatccgct
     5161 taaacgacct tcgttatcta cgattggtaa tttttcgatt ttatgttttt gcaaaatctt
     5221 ttctgcatct tttagagagg tacctactgg ggcagtcact aagttgtctt ttgtcatcac
     5281 atcagcaatc ggcatgtgat aatctgttac aaaacgcatg tcgcggtttg taattatccc
     5341 taccaatttt ctgttttcaa gtgtttcaac gattggcaca ccactgatac gatatttgct
     5401 catcaattct tctgcgtctg ctacaagatg ctgtggcgtt aagaagaacg gatcaatgat
     5461 tacgccactt tcagaacgtt tgacttttct tacttcatcc gcttgctggc tgatcgtcat
     5521 gtttttatgg atcacaccta gtccgccttg tcttgccata gcaatggcca tcttgctgtc
     5581 ggtaacagta tccatacttg cactgatgat tggaatattc aacttaatat ttttagctaa
     5641 ttgtacgctc atatccactt cgtttggtaa cacgtgactt tctgctggta tcaataagac
     5701 atcatcaaaa gtaaatcctt ttttcgtgaa ctttgtttcc cagttggaca tttttagtac
     5761 cactcctcta atttagttta tctattaaaa taattgattt tttcatttaa gtcaatgatc
     5821 tacttggaat caagttagct ttctcataga ataccaatat aaagcgttta catctgtgtg
     5881 tattaaaaga gattgtaaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     5941 acagcttctc atgtttttga attggttggg tttattaccg cagatgcaaa cttttgaaca
     6001 ccgtttagtg gagaggctgt gaaaaagctg cttagtttcg agtagtaaga aaaattttgt
     6061 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     6121 acaccgttta tcgtaaaaag ggactgtgac aagacttttg tcacagtccc ttactattat
     6181 ttaactatgg ataaacaaaa tgaggttctt tactacccct agatatctag caaaatagct
     6241 gatctttttg ctagtttatc ttcttgggac ggataaactt ccaataatcc cgtatttttt
     6301 cttcaagtag taacggattg ctaaagcagt tgccccgata atgataacta ctactggatc
     6361 taatactgga ttgataactg gtggcaatag tgttgtcgca gaaaacagta cgacccaaag
     6421 aagtgtcgtt ccacctagga taagcaatgt cttacccatt cctggtttct tgcttcgatc
     6481 tgctccggga tattcatatt gatagatgta tttgtacatc atataaaaga cccagccacc
     6541 gattgcagaa cctagaacga gtgtagaaaa gccataagct ggcgttgttc cacgtgaaaa
     6601 caatccggta atcgcaatca tgatacttaa tagaccaagc aacagcaacg tattatctaa
     6661 ccacatcaat aaaggggtgg atgaattttt tacttctgct ggcttattga tgattgcttc
     6721 cgttctttca gagactgtcc caaacaattg acgggctgtt cttccgcctt tttgttctgc
     6781 aactaaagta ggcagaatgt catgaagggc ttttactttt tcttcttcag acaaattcgc
     6841 tgcttctaaa gattttttca aatcaaaaat gtactgttga tttcttttgg ttaattttgt
     6901 ttctaaacta ttgttttctt ttacgattgc tcgtaattgt tctggttcca ttatctaata
     6961 gtcctcctta gccgttcctt ttctcaacgg tcgtcacaaa agacattcta ccatattcta
     7021 cagctagttt gatagccctt ttacacatta aagcggaata acattacgtc gccgtcttgt
     7081 acgacataat cttttccttc taatcgaaca cgtccagctt cttttgctgc atgcatgttt
     7141 ccgtaatgat ccaagtcttc aaaagagaca gtttctgcac ggataaatcc gcgttcgaaa
     7201 tctgaatgga tgattcctgc tgcttgtggt gctttgattc cttttttgaa tgtccaagca
     7261 cgtacttctt gttccccagc agtaaagtaa gtagctaagc ctaacaaatc ataagcagca
     7321 cggatcaact gatccaaacc tgattcttcg attcctaaag cttctaaaaa ttccgctttg
     7381 tcttcttcat ctaattctgc gatttcttct tctgcacgag cactgaccac gatcacttct
     7441 gcattttctt ctgcagcaaa gttgcgcact tcttgtacat aatgattcgc gtctgcatct
     7501 gctacgtcat cttctgagac attggctaca taaagaattg gttttgtcgt caataaaaat
     7561 agtgatttaa cgattttttg ttcttcttca gtaaattcga tcgttcgggc agattttcct
     7621 tcttccagta caggtttgat tttatccagt acagctagtt ctgccactgc atctttatct
     7681 ttggttttag caacttttgc tacacgagta tagcgtttgt tgatagattc taagtctgct
     7741 aacactagtt ccaagttgat cgtatcaata tcttctagtg gatcaacacg tccttctacg
     7801 tgagtaatat tatcatcatc aaaacagcga acaacgtgac aaatagcgtc tacttggcgg
     7861 atatggctta agaattgatt tcccaaacct tctcctttac ttgcgccttt tacgattcca
     7921 gcaatatctg taaattcaaa agtcgtcgga acagtttttt tcggatgtac aagttctgtt
     7981 aagcgttgca aacgccagtc aggtacttct accatcccta cgttggggtc gatcgtcgca
     8041 aagggatagt ttgctgcttc tgctcctgct tttgtgatcg cattaaacaa agttgatttt
     8101 cctacgttgg gcaacccaac aattccagca gtcaatgcca taaatttatt cactctttct
     8161 ctgcattttt ttcaattact ttttttaatt tcttttcaaa atctcgacgg gtcatcatga
     8221 cgatatgtcc gcaattcata catctgattt tgatatctgc gcccattcga atgacttccc
     8281 agcgattcgt ctggcaagcg tgaggttttt tcatttcaac gatatcgtgc aagtcgtaca
     8341 taaaaatagc ctcctttttg tttattcttc atcaaattga atattcaaga tatcaagaat
     8401 tcgagtcaaa tctttttgag aaagatattc aatctcgatc ttacctttcc catttttttc
     8461 ttggatagct acacttgtac caaatttatc catcaaacga tcttcgcttt ctcggatata
     8521 atatggtttt tcctttgcta gccttgggat ttttttcttt tcttttccat cattttgatt
     8581 taactcagaa acaagctgtt ccagttggcg gacagttaaa ttttcagtta ctgcacgatt
     8641 ggctaatttc aagatcaatg ttttattttt taaacctaac agcgtacggg cttgtcccat
     8701 agataaacgc tgatcttgta ccatttcctt gacaagatcc ggtaaagaaa gcaagcgcaa
     8761 ataattggcg atataaggtc tgctctttcc taaacgacta gctacttctg cttgtgttag
     8821 tttcaggttt ttcatcagca tctcatatgc ttctgcttct tctaacggat tcagatcttc
     8881 tcgctgcaag ttttccagca ctgctacttg catcatcgct tcttcgtcaa agtctctaac
     8941 gatagcaggg atcgtttctt tttcagctag tttggacgct cgaaaacgtc gctcccctgc
     9001 aataatctca tatcctttta ctgcggaacg gcgtacgatg atgggttgaa agacacctga
     9061 ttgttcaata gaattagcta gttcctgcaa agatacttct tcaaatgttt tacggggctg
     9121 atacggattt ggtcgcagct cattcaatgg tagctggaca acggtatcat cttttacatc
     9181 caccgtctct aagttttcta aatcttgaaa cagtgcatcg attcctcggc ctaatccttt
     9241 acttttcttc acgacttacc acttcctttg ccaatgcttg gtagacttca gctcctcgag
     9301 aacgtggatc gtagtcgatg atcggttttc catgactagg tgcttctgat aaacgaatat
     9361 ttcttggaat gatcgtttcg taaacttttt cgcggaagta tttacgtact tcttctacga
     9421 cttcatttcc cagatttgtt cgtgcatcat acattgtcag taagactccc tcgatctcta
     9481 attctggatt aaagtgtttt tggactaaac gaaccgtatt cagtaattgg cttaatcctt
     9541 ccaatgcgta atactcacac tgaacaggta tcaaaatcga atcacttgct gtaaatgaat
     9601 tgattgtcag atggccaaga gaaggcgggc aatcgatcaa aatataatca tactgactgc
     9661 ttacttctgc taaagatcct tttaatcttg attctcttgc catcattgac gtcaattcga
     9721 tctctgctcc tgctaattgg agagtcgcag gcacaatact caaattttcg tgttcagtga
     9781 tcaaagttgc ctcatcaatt ggcaattcat tgactagaac atcatagatg tctcttgtca
     9841 catcaggttt tctgatcccg acaccactgg tagcatttcc ttgtgcatcc atatccacta
     9901 gcaatacttt tttgccaagg ctagcaagac aagcacctag attcaccgtc gtcgttgttt
     9961 tgccgacgcc acctttttgg ttcgcaacag atattatttg tgccatctct tctcctccta
    10021 tttgattggc tgcttattag gcagtccggg ctttcttggg tatttctttg gggttggttt
    10081 ctttttttga ataacgatga tatgccgttg atcagagaca ttcggcaagg taaccgcctc
    10141 ttctttgagg aatttaccac ctaataaagc aatcgcttgt tttgcttctg tcaattcctc
    10201 ttcacttttt gctgctttca atgcataaaa atagccttct tgtttagcca agggcaaaca
    10261 caattccgct aacacattca aacgagcaac cgcacgagca gtgacaaggt caaatgatgc
    10321 acgaaagtga ggattttgcc caaatgtttc tgcccggtca tgatataagg aagcatcgat
    10381 tcctaataat cgggttaact cagataagaa ggttattcgt ttattcagag aatcaacgat
    10441 cgttatctgc agttctggaa aggcaatttt taaaggtatg ctaggaaacc ctgcccccga
    10501 accaacatcg cataaagata aaggttgcgt aaagtctact tcaaatgcta atgtaagtga
    10561 atcgtaaaaa tgcttcagat atacgtcttt ttcatccgta atagctgtaa ggttcatctt
    10621 ttcgttccat tcgactaatg tccgataata aatagcgaat tgattcattt gatgatcggt
    10681 aagctctatc cctttttctg ccaaggattg gcgaaattct tctggtatca taaatattcc
    10741 tttcattgtg aaacagcaat tgtttcacag attctctccc tactttaacg caaaatctct
    10801 agacatgcaa tccttccata tggattctta aaaatttgaa aagtaagaaa agccgataga
    10861 ctttttcttt ctagtatgac tcataaaata tacacagaag agagaactag ctttgagcaa
    10921 tttctgcaaa aactagttct ctcttctatt tattattacg tattacgctg gttcactgac
    10981 tttcgcaatc ttcccttgtt cgatatagac cattaggata cttaggtctg cagggtttac
    11041 gccactgatt cggctagctt gggcaattgt ttctggttgg attttctgca atttttgttt
    11101 tgcttctgtt gctaatccgt tgattgctgc gtagtcgatg ttttctggaa tctttttcgc
    11161 ttccatgcgt tttaattttt ctactttttc catggctttt ttgatgtagc cttcatattt
    11221 gatttggatt tctacttgtt cgatttcttt tcgtgtcaat tcttcatttt ctggaataaa
    11281 ccgtagaagt tctgcataag taatctcagg acgtcttaaa aagtcgcttg ctagtacacc
    11341 atcttttaat ggtgccgcat taatcgttgc taaaaagtct tggacttctt ctgttggttt
    11401 gattcgtaca ttcatcaaac gtttgatttc ttgttcaact gcttcttttt tcttcaaata
    11461 attcttgtat tgatcttctt ttactaagcc tacacggtga cccatttctg ttaatcgcaa
    11521 atctgcatta tcatgacgta aaagcagacg gtattcagca cgtgaagtca gcaaacgata
    11581 aggttcgttc gttccttttg ttaccaaatc atcgatcatg acaccgatgt aaccgtcact
    11641 tcgtttgatc actaaaggtt cttttccctg aactttcaaa gctgcattga taccggcaat
    11701 cagcccttgt ccagccgctt cttcataacc gcttgtacca tttgtttgtc cagctgtata
    11761 aagattttca acgactttag tttctaatgt cggacggagc tggtgtggct ctactacgtc
    11821 atattcgata gcatatcctg tacgcatcat ttctactttt tctaatccag caatagaatg
    11881 aagcatgttg acttgaacat cctctggtaa agaagtagat aaaccttgaa catagacttc
    11941 ttccgtattt aagccttctg gttccaaaaa taattgatga cgtggtttgt ctgcaaaacg
    12001 cacgatctta tcttcgatgg atggacaata gcgagcgcct accccttcaa cgatccctgt
    12061 aaacattggg gcacgatgta agttgtctcg aataatttgg tgagtcgtct cacctgtata
    12121 ggtcagccag caagattcct gatctagttt ataggcacta tctggtgtgc tgaagctaaa
    12181 atgattcggc tctttgtcac caggctgctc ttctgtaaca gaatagtcga tcgtacttga
    12241 ttttactcga ggcggtgttc ctgttttgaa ccgttcgata gcaaagccaa gttcttttag
    12301 atgattcgct agtccaatag aaggttgaga gttattcgga cctgatgaat atttcaattc
    12361 accgataatg atttctccgc gaagtgctgt tccggctgta ataacaactg ctttggcccg
    12421 gtagctcgct cctgtggacg tcactactcc atgacaaaca ccatcttcga cgatcagttc
    12481 ttcgacgatt ccttgtctta gcgtcaaatt tggttctttt tcgatcgtat gtttcatttc
    12541 tgttgaataa gcatgtttgt ctgcttgtgc tcgcaatgct cggacagctg ggccttttcc
    12601 tgtgttcaac atacgcattt ggatataggt tttatcgata ttcttaccca tttctccgcc
    12661 taacgcatcg atttctcgaa cgacaacgcc tttagctggt ccgcctacag aaggattaca
    12721 tggcatgaat gctaccatgt ccaaatttat tgtcagtaat aatgtttttg atcccatacg
    12781 tgcagcagca agtgcagctt ctgaaccggc gtgtcctgcg ccaacaacga tcacgtcata
    12841 cgcatctgct tcataatgtt ccaatcgttt gttcctcctt ttattttcct aaacaaaatt
    12901 gactgaataa ttgagtgatt agctcatctt gtacactgtc gccgacaact tctcctagat
    12961 agtcccagca acgcgtcata tctatttgaa cgagatccac aggcattcct gaatcaatac
    13021 ctgcaatgac ttcttcaagt gagagggaag ctttttctaa caacgcgata tggcgggtat
    13081 tagaaacata agtagcgtct ctttctcctg tttctccgct gaagaataaa tcagaaatag
    13141 ctgattccaa acgatctagt ccgtcgtttt tagctacgga aattgaaaaa actggttcat
    13201 tttcgatcaa tttcttcaat ttttcttgtt ccagctgtgc tggcaaatct gttttattta
    13261 gaagaatgat ccgtttcaat ccacttgtcg cttccaataa ttgttcatct tctgctgtca
    13321 gtggttcgct ttggtttaga acaagtaaga tcaagtctgc ttctgctaat gcttttcggc
    13381 ttctttcgac cccgatccgt tccaccacat cttctgtttc ccgaattcca gctgtatcga
    13441 ttagcttgag cggtaccccc cgaacattga catactcttc gattacatct cgagtcgttc
    13501 ctgcaatatc cgtaacgatt gctttttctt ctcttaacaa atggtttaac aaacttgatt
    13561 tgccgacatt tggccggccg atgattgccg tacttaaccc ttcccgcaga accttccctt
    13621 gcttggatgt agcaagtagc gcttggattc tttgttggat catctgcgct ttttcaagca
    13681 ataacttcgt agtcaattct tcgacatcat catattctgg ataatcaata tttacttcta
    13741 cttgtgctaa tgtctctaaa atctcttgtc tcaatgagcg gatcaaagca gacagattgc
    13801 cgtctaactg attaagagct agtcccattg ctttgtctgt ttttgcccga atcaaatcca
    13861 tcacagcttc tgcctgtgaa aggtcaacac gaccattcaa gaatgctcgt tttgtgaatt
    13921 cgcctggctc agctaatctt gcaccttctc ttaacaatag ctgcaaaatt tgattgacta
    13981 caacgatccc accgtgacaa ttgatttcta ctacatcttc ccgcgtaaat gtctttggtg
    14041 ctctcatcac ggagaccatg acttcatcga caagctgatt gctcttggga tcaacgatat
    14101 gtccataatg gatcgtatga cttggtactt ctgataatcg tttgtttcca gattgataaa
    14161 ctttatctgc tagttgcaca gcttgatcgc cacttaaacg aacgatactg attgcacctt
    14221 ctccaggtgg ggtagaaatt gccgcaatcg tatcaaattc taaagtaatt tcagccattt
    14281 tttcacttcc tttttcattt tgcgcacaaa aaaagtgccc actccaccct ttatgcacaa
    14341 taaacggtga acttcgcact ttgactgctt gttcttgatt aaattctggt catagattat
    14401 cacacttgct ataaggaatc aagtgtttaa aacaattaga tgaagttgag cctgttctgc
    14461 ttccattacc ttatatttaa ttgtccttgg atgtctcttt gcgttctttg gtttattcga
    14521 agagaagata ctgtataaac aaggctttat ctttcttaaa aaaaacggta cactaaatgg
    14581 tagcgctatc taaactatac acttttgaaa ggagaaaata tgtgagcccg ttcagatctg
    14641 ctgcattgga agaagaaagt gaccctcgga tcaacgatgt cgttttatct acagggaata
    14701 tcaaccagaa atatatcgtc cgcgatatcg tttttgttgt ggaatgctta gaagctgatt
    14761 tgtttgaccc atcttttccc aaagatgacc ttttcttaac aacgaagagg aaattaaaga
    14821 cgaaggtact tgaatatggt gcgaatgcag taatcaactg tcattttgag tatcagcaaa
    14881 aaaaacgcga cggaaagtat tttctggaac tttttgctta tggaacagtg atccaattta
    14941 tccaaacaac gattggttaa atgaaacaga caaaaaaagc ggatcaaaac tgaagacagt
    15001 ttcgatccgc ttttttctat caaagttagt gactacttca tcgtatactc tgataagaat
    15061 gatttctagt aaaatatact tggtttgaaa aactttgtca acgtgattta ttcaaaaagt
    15121 aatggaatca ataatatttt ttagcaggtt ctactactaa ataacgataa ggttcctcac
    15181 cttccgaatg tgtttttaca tgttcatctt tgcttaatgc agcatggatt tgtttacgtt
    15241 caaatgcagg cattggttct aagaaaacag gctgccctgt ccgttttact ttttcagccg
    15301 tgcgatcagc taaacgctga atgatctctt ggcgcttttc acggtaattg cctacattga
    15361 ctactacaga cagcttattt cttgctaaac gatggatgaa gacttgtgcc agatattgca
    15421 atgcattcag tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt gtttctaaat
    15481 ggaaaacaac catattattt gattctcttt ccattttgac aagagctgga gcatcaagct
    15541 gttttgaaat ttcagtaaga taaactgcca attccgttaa agctgcttca tcacctagct
    15601 cacttacttc ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg atctcagctg
    15661 ttggttcttc ttccaaaatt tcttctacag tctgttctac tgtttcagaa actttttcgc
    15721 tgatacttgg ctggatcgaa agccgtgcat tctttcgacc cattcctagg aaaccttttt
    15781 ttccttcttc cagtatttcg atttcgactt ggtctttggc caaacccaaa tcagccagac
    15841 ctagttggac agcttcgtct attgttgcag cttcataaat cggcattagc attacctccc
    15901 ctattatttc cgttttttct taggattcat tgctttctta agagcacgtt ctctttcgcg
    15961 gattctccgt gcttcttctt cacgctcttt tcgaattttg aaagggttgt tgatcagcag
    16021 cgtttgtaca acttggaagg cattggaaac tacccagtat aatgaaagac cgctggctaa
    16081 attgacaccc atcactaaaa tcattacagg catcacgaaa ttcatgatct tcattgatgc
    16141 attcgattca atttgactca tgctagataa atacgtactt gcaaaagtaa agattgctgc
    16201 taaaatcggc aaaataaatg ttggatccgc gttccctaag ttcaaccata agaaatgccc
    16261 ttgtttcaac tctggtacac gtgaaataga ttgccataac gccatcaaga ttggcatttg
    16321 tactaataaa ggaagacaac cagcatatgg attgacgcca ttttctgcat ataaacgctg
    16381 ctgctcttct cttaacttac tttgtgtttc cggatctctt gaactatatt gttgctgcag
    16441 tgcttttaat ttcggttgta attcttgcgt tttacgcatg ctctttgttt ggaaatgcat
    16501 caacggcaat aggatcacgc ggatgatcag tgtaaataaa atgatcccta tccctgcatt
    16561 accaaatgac aacgccttga tcgcctcagc aaagtaataa acaatataac gatcccaaat
    16621 tcctgtactc tgtgcgctga cttccccagt tccgcaaccg gataagacga tgaccaaact
    16681 aacgataccg gccatcaaaa gtaaacgttt gtatttcttc acttactctt ttcctctctg
    16741 tcgattattt ttgcaagttt taagacgtgg actaaattgg agcgaacctc atcataagtc
    16801 aaccccttca cacttggtct agcaatcaca ataaaatcca actccggatc aatttcttct
    16861 tttatagaat aaactgctgc tcggattttt cgtttgactt cattgcggtt taccgcgttg
    16921 ccgacctttt ttcctacaga taaaccaact cgaaaatgag gctgtcccgg tttttccaaa
    16981 cggtaaacga cgaatttgcg gttagcaaaa gaatctcccg tatggaacac ggcttgaaac
    17041 tctcgttctt tttttactcg atacgctttt ctcattagtt ttctttcctt ttctatctct
    17101 cacttacgct cataatcata ccataattac gattgtcctt tcatcatttc cctaaaaaag
    17161 agtataaaaa aaaccactga aaccccagtg gcctaagctg aaagaacttt tcttcctttg
    17221 cgacgacggc tagctaacac acgacgtcca tttttagtac tcatacgttt acggaatccg
    17281 tgaacttttt gacgtttacg tttatttggt tggtatgttc ttttcatata ttccacctcc
    17341 aaaatgttct tatctatttt ataaacacag acatactttg atagtataac gacaaagtag
    17401 tgattttgtc aatataaaat agaagatcga ttgttaaaac ttatccacaa gattattttt
    17461 ttctatggga atcctatgtg gaaaaagtta tgcacaggga ttatccacag ataataaaag
    17521 gctggtttta cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag gcgatttcct
    17581 gtgtattccg tctcctaact attggtatat ctgtattttt tatccacaga cgcaagagga
    17641 aatctattgg tttttacgag ttttccacct gtgcattagt ggtgtggaaa actttttttg
    17701 acaactgtgt ttttctcttt tttctttttg tcgattgtgt ggataattgt aattaaaaaa
    17761 tttttttctt cggtttctgt ggaaaactat tgaaaaaaat gctaaattag tactagttat
    17821 tcacttttta atgaaggagg aggccttata tggtatccct cgatgcttta tggaatgaat
    17881 taaaagcaac ataccaaaag gatttatcgc ctgctagcta taatacatgg atcgaaacag
    17941 cgaaccctcg aacccttgat caaaaccagc tcgttgttga ggttcctagc aagattcata
    18001 aagaatattg ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat atgttatcag
    18061 gaaatgagat tatccctcgc tttattactg gcgaagaagc agagcaagaa gaagttatag
    18121 aagaaaaaaa tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg ctgaacccta
    18181 aatatacctt tgatacgttt gtcattggta aaggaaacca gatggctcat gcagcagcgc
    18241 tcgttgtagc agaagatcca ggttctattt ataacccgct cttcttttat ggaggcgtgg
    18301 gacttggtaa gactcacttg atgcatgcaa ttggtcatca aatgctgcaa agtcagccta
    18361 atgccaaagt aaaatatgta agcagtgaaa cttttgctaa tgatttcatc aattcgatcc
    18421 aaaacaaaac agcagaagag ttccgtcaag aatatcgaaa tgttgattta ctgctagtgg
    18481 atgacattca attttttgca gaaaaagaag ctacgcaaga agagtttttc catactttca
    18541 atgccttgta caatgaaggg aaacaaatcg tactaaccag tgatcggttg ccaaacgaga
    18601 ttccgaaact gcaagaacgc cttgtttctc gctttgcatg ggggctgtct gttgatatta
    18661 caccgccgga tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg gctgaacgtc
    18721 tagagatccc agatgacaca ctaagctaca tcgcaggaca aattgattct aacattcgtg
    18781 aattagaagg tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt gaagatattt
    18841 cgactagtct agctgcagac gcactgaaaa cgttaaaatc aggaaaagga catcctcaat
    18901 tgtcaatcct gcagatccaa gaagaagtgg caaagtatta tcacatccaa ttgaaagatc
    18961 tcaaaggaaa gaaacgcgtc aaatctattg ttgttcctag acaaatcgct atgtatttgg
    19021 cacgtgagct cacagataat tctttaccga aaatcggagc tgaattcggt ggcaaggatc
    19081 atacaacggt tatccatgct catgaaaaaa tccagcagct gatggattct agtgtcagta
    19141 tgcaaaatga agtatcagaa atcaaaaact ttttactaaa ttaatttgtc gattctatca
    19201 actgaaaata gcatgtggat aaaagaaaaa aacgttcaaa agttatacac actttttcca
    19261 cagctgtttt ttcgtttctc tcttacgttt tttgagtttt ccacagaatc aacatgccct
    19321 attactttta ttattctttt taactaataa taaataaaca aacattcaaa ggagatacat
    19381 catgaaagtt actttaaacc gagctagctt tatgcaggaa ttgcaaactg ttcaacgagc
    19441 tatttcaagc aaaaccacga tccctatttt gacaggtgta aaaatcacac tgacacaaga
    19501 aggtttgact ttgacgggga gcaacgctga tatatcaatt gaaacttttt tgtctgttga
    19561 aaacgaaaaa gcaaatatgc aaatcgaatc tactggttcc attgttttac aagcacgttt
    19621 ctttagcgaa atcattcgga gacttcctga agaaacattt actttagaag ttttagaaaa
    19681 taaacaagta gcgatcactt ctggaaaagc gaattttatc gtaaatggat tagatgcaga
    19741 taactatcct catcttcctg ttgtcgaaag ccataaccag atgaaattac ctgtacacgt
    19801 attgactaaa ctaatcaacg aaacagtttt tgctgtctca caacatgaga gtcgtccaat
    19861 cttgacaggt gtgcatttca ttttatctga taattcttta ttagctgtag ctactgattc
    19921 tcaccgtcta agtcaacgcg tgattccagt agaacaagcg gctgatcatt ttgatattgt
    19981 tattcctgga aaaagtttga tcgaattatc tcgctcatta acaaatgaag aagaaatcgt
    20041 cgaaatcagc attatggaaa accaagtgtt gttcaaaaca gaaacgatgt atttctattc
    20101 ccgtttgcta gaaggaaact atccagatac caatcgtttg attccttcaa gttttaatac
    20161 agaggttgaa ttttctgttc ctagcttttt agcggcgatc gaacgggctt ctttactttc
    20221 tcatgaaggc cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg ttgttttata
    20281 cggaaattcg ccagaaatcg ggaaagtaga agaaagcttg agttatacag caagcagtgg
    20341 cgatccatta gatatttctt tcaatccgga ttatatgaaa gcagcgttgc gtgcatttgg
    20401 agatatgagc atcaaagtga aatttatctc tgctattcgt ccatttacat tggaaccaac
    20461 ggaagatggc gttcaattta ttcagctgat tactcctgta cgtacaaatt aacaatgatt
    20521 ttcaatgttc ttgaaaattt aattgaaaag aaatagaaaa aataaaattt taaacagaag
    20581 gccctaaaat ctcttttaag gctttctgtt tatttttgtt taaatcacct tttttagatt
    20641 aaatcgtctt atatggaaaa atatatggat aaatttgttt tattgcgaaa aaaaaggtat
    20701 aatagagtat acttttaata atgaaaatga gggatgggca ttgaaacaac aagtcattct
    20761 aaatacggaa ttcatgacgt tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg
    20821 cggacaagca aaatggtatt tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa
    20881 tcgtcgtgga cgcaaacttt atcccggcat gatggttgaa gttccagaag taggaacttt
    20941 ttttatggta aaaaaaggaa acgacgataa tgaggctgaa taagctgtat ttaaaaaatt
    21001 atcgaaatta cgaagagctg aacatcgaat tttccaagaa cctcgtcatc tttttaggag
    21061 aaaatgcgca aggaaaaacg aatcttttgg aaagtatcta cgtattagct atgacgcgaa
    21121 gtcatcggac aagtagtgag caggaattga ttggctggac tgacgatcag gcgatgattc
    21181 aaggagagat cacaaaaggt tcatccactc ttccattaga aatcttatta tctaaaaaag
    21241 gccgaaaaac caagatcaac catatcgaac agaaaaagtt aagtacttac gtgggacaaa
    21301 tgaatgtcat tttatttgca ccagaagatc tttctttagt aaaaggcagc ccgcagatca
    21361 ggcggaagtt tttagatatg gaattaggcc agatcaatcc aatctatctt tatgatcttg
    21421 tccaatacca agcaattttg aaacaaagaa accagtactt gaagcaattg aatgaaaaaa
    21481 aacaaactga tttgctttat ttggatattt tgtccgaaca actagcagcg tttggcagca
    21541 aagtattgaa agcaagagca cagttcgtca agcgattgga gttttgggct aattctttgc
    21601 atcagcaaat cacccatcaa aaagagcagc tggaaatcga gtatctcacc gcagtcgatt
    21661 ctttagaaac acatacgcaa gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa
    21721 ataaaaagaa agaccttttt cggggaacaa cgacagttgg tcctcatcgt gacgatcttt
    21781 ccttttttat taatcagaaa aatgtgcaga cttatggatc gcaaggacag caaagaacaa
    21841 ctgcactaag tgtcaaatta gcagaaatcg atttgattaa agaagagaca ggggaatatc
    21901 caatcctttt gttagatgat gtgatgagcg agctggatga taatcgtcaa ctccatctgc
    21961 tagaaacaat cgaaggaaaa gttcaaacgt tcttaacaac aacgacactt gaccatgtaa
    22021 aaaataaaat gactgttgaa cctgagattt tttatgttca acaagggaaa ctaaaaggag
    22081 aagaaactga atgacagaag aaagaagttt agtagaacgc gctaaagagt atgatgccag
    22141 tcagattcaa gtacttgaag gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg
    22201 ttcgactagt tctgagggtc tgcatcacct tgtttgggaa attgtcgata attcgatcga
    22261 tgaagtatta gcaggttttg caacgaaaat ccatgtgatt attgaaaaag acaacagtat
    22321 caccgttatt gacgacggtc gggggatacc tgtagatatc caggcaaaaa ctggacgtcc
    22381 tgcagttgaa acggtcttta ctgttttaca tgctggagga aaattcggcg gtggtgggta
    22441 caaagtttct ggtggacttc acggagtagg atcttctgtt gtaaacgcgc tttctacaca
    22501 gcttgatgtc aaagtataca aagaaggaaa tgtgtactat caagaatatc gtcgtggagc
    22561 tgtagtggac gacttaaaga ttatcgaaca aacggatcgc catggaacaa cggttcactt
    22621 tacgccagac cctgaaattt ttacagagac aactgagttt gatttttcaa aattggctac
    22681 ccggatccgt gaacttgctt tcttgaaccg cgggatgcgt atatcgattg aagacaaacg
    22741 agaagaagaa ccagtcatca atgaatacca ttatgacggc gggatcaaaa gttatgtcga
    22801 atacttgaat gcaaacaaaa ccgtgatttt tccagaacct gtatatttgg aaggtgaaca
    22861 gcaagatatc gcagttgaag tctctatgca atatacagac ggctaccatt ccaatatcat
    22921 gagttttgcc aacaatatcc atacgtatga aggtggaaca catgaatcag gattcaagac
    22981 tgctttaact cgtgtgataa atgattatgc ccgaaaacaa aagctgatga aagaaaatga
    23041 cgacaacttg accggtgaag acgtgcgaga aggattgaca gctgttattt ctatcaagca
    23101 tcctgaccct caattcgaag gacaaacaaa aacaaaattg ggcaattccg aagtacggac
    23161 agtcactgat cggttattta gcgaacattt catgaaattc ttgctggaaa acccaagtgt
    23221 gggccggcaa attgttgaaa aaggattact agcttccaga gctcgtctag cagcgaaacg
    23281 tgcgcgtgaa gtaacgagac gtaaaggtgc tttagagatt agtaatctgc caggaaaatt
    23341 agcggactgc tctagcaacg atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc
    23401 ggccggcgga tcagctaagc aaggtcgtaa tcgagagttt caagccatct tgcctattcg
    23461 tgggaaaatc ttgaatgttg aaaaagcaag catggacaag atcttggcaa atgaagagat
    23521 ccggtcttta tttactgcta tgggtacagg gtttggagaa gattttgatg tatcgaaagc
    23581 tcgctaccac aaattagtta tcatgactga tgccgatgtc gacggcgcgc atatccgtac
    23641 attgctgctg actctttttt atcgttacat gcgtcctatt gttgaagctg gatacgtcta
    23701 tattgctcag ccacctttat acggggtaaa acaagggaag aatattacgt atgtacaacc
    23761 aggaaaaaat gcagaagaag agttgaaaca agtcgttgct tcacttcctg cctcaccaaa
    23821 accaagcgtt cagcgttata aaggacttgg agaaatggat gatcatcaac tatgggaaac
    23881 aactatggac ccaagcaatc ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc
    23941 agatcagata tttgaaatgt tgatggggga tcgagtagaa ccccgtcgtg cattcatcga
    24001 ggaaaatgca cactatgtga agaacttaga tatttaatca tgaaggagga aaaagaatga
    24061 gtgaagaaat cagagaaaac atccatgatg tcaatctaac cagcgaaatg aaagactcct
    24121 tcatcgatta tgcgatgagt gttattgtcg cacgtgcact tcctgatgtc agagatggtc
    24181 tgaaacccgt tcatcgccgt attttatacg gcatgaacga attaggtgta acaccggata
    24241 aagcccataa aaaatctgcc agaatcgtcg gagatgtcat gggtaaatac catcctcatg
    24301 gagacattgc gatctatgag tcaatggtac ggatggcaca gccattcagc taccgttata
    24361 tgctagtgga tggtcatgga aactttggtt ctgttgacgg tgacggtgcc gccgctatgc
    24421 gttataccga agcacggatg agcaaaatcg ctacagaaat gctgcgtgat atcaataaga
    24481 acactgttga tttccaaagc aactatgatg atacagaaaa agaaccagtt gtactacctg
    24541 ctcgcttccc gaatctttta gtgaacggaa caacggggat cgccgtaggt atggccacga
    24601 atatcccacc tcataacttg tctgaggtag tagcggctat cgatttgctg atggataatc
    24661 ctgatgtgac gaccaatgaa ttgatggaag tattacctgg accagatttt ccaacaggtg
    24721 gtttggttat ggggaaatca ggaatccgaa gagcgtatga aacaggtaag ggttctatta
    24781 ccgtacgtgc aaaagttgag attactgaga tgccaaatgg taaagaacgg atcatcgtga
    24841 cagaattgcc ttatatggtc aataaagcaa aattgatcga acggatttct gaactgcaca
    24901 gagacaagcg tatcgaaggg atcacagatt tgcgggacga atcttcccgt gaaggtatgc
    24961 ggatcgtcat tgacatccgt cgtgacgcca gcgcatccgt tatcttgaat aacttatata
    25021 aattgacatc cttgcaaaat tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc
    25081 cgaaagtttt gagcttgaaa caaatccttg aaaactatat cgaacatcaa cgcgaagtca
    25141 tcacacgacg gacggtcttt gaaaaagaaa aagcagaagc aagagcacac atcttagaag
    25201 gtttgcgtat tgctttggat catatcgatg aaatcattgc gattatccgc gggtctaaat
    25261 cagatgacga ggcaaaagca acaatgatcg aacggttcga tttatccgat cgccagtctc
    25321 aagctatttt agatatgcgt ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa
    25381 atgaatacca agaactctta aaactgattg aagatttaac tgacattctg gcaagaccag
    25441 aacgtgttac agaaatcatc aagaatgaat tagcagactt gaatcaacga tttggtgata
    25501 aacgacgcac agaattacta gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg
    25561 aagaagaaga aatcgttatt acattgacaa acaatggtta catcaaacgt ttggcaaata
    25621 atgaattcag agctcaacga aggggtggac gtggtgtcca aggaatgggt atccacaatg
    25681 acgacttcgt caaaaatctg gtttcttgtt ctacccatga tacattgtta ttctttacga
    25741 ataacggaaa agtgtatcgg gcaaaaggat acgaaattcc agaatacggg cgtacagcca
    25801 aaggaatccc aatcatcaat ttactaggta tcgattcgag tgaaagcatc caggcaatca
    25861 ttgcggtgac gggagaagca gaagaaggcc actatctctt ctttacgacg cgtcaaggta
    25921 cagtaaaacg tacttctgtc actgcttttg ctaacatccg aagtaatggt ctgatcgcaa
    25981 ttggtttgaa agaagacgat gagctcgtca atgtgctact gacagatggt caatctaata
    26041 tcattattgg aacacataac ggctactctg tcactttcaa agaagaagca gtccgcgata
    26101 tgggacgtac agcttccggg gtcagaggga tacgcctgcg tgaggaagat tatgtcgttg
    26161 gggcagcctt aatgaaggaa aatcaagaag tattagtgat cacagaaaaa ggttacggaa
    26221 aacggacaaa agtatcggaa tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg
    26281 cgaacatcac tgaaaaaaat ggtccactag ctggattgac cactgtttct ggagatgaag
    26341 acatcatgct gattacagat aaaggtgtca ttatccgttt caacgtatcg actgtttctc
    26401 aaactggacg ttcaacatta ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg
    26461 tcacgatggc agcagtcgaa ccagaagctg cagaacaaac agttgaagaa caaggatcag
    26521 aagaataacc atcaagacta agtgtgtaag ctagcaagaa taaccgatta aaaaatctgg
    26581 ttattcctgc tggctttttt gattagttga atttctctta aaatattcga aactgttcat
    26641 tctgtattgc ttttgaccaa aatagagtgt ataattagag cttgtgagta gtctaattaa
    26701 ttagataact ctccttgctc ttaggaacct aagagccgaa agtccacagg gaggtgaata
    26761 tcaatggaaa acacgaaata tgaaatcatg tacattattc gtccaaacat tgatgaagaa
    26821 gcaaaaactg ctttaatcga acgtttcgat gcaatcttga aagataacgg agcagaagtt
    26881 atcgaatcaa aagattggga aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa
    26941 ggtatctatc acatcgttaa agtatcttct ccatcatcag caaatgctgt caatgaattt
    27001 gaccgtcttg ctaaaatcaa cgacgatatc attcgtcaca tgattgttaa agaagaagct
    27061 taattgttgt ttcatgtgaa acaatagaaa acgatgagag gagatgacta ttttgattaa
    27121 taatgttgta ttagttggac gtctgacgaa agatccagat ttgcgttaca cagcaagtgg
    27181 tacggcagtc gcaacattta ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa
    27241 tcgcgaggct gattttatca actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa
    27301 ctatgctcgt aaaggaacac ttttaggtgt tactggacgt atccaaactc gttcttatga
    27361 taatcagcaa ggacaacgtg tatacgtgac agaagtagta gctgacaact tccaattgct
    27421 ggaaagtcgt tctgcatctg aaaatcgcca acaaagcggc ggattccaaa gttctggtca
    27481 atcagctggc ggatttggcg gaaacaataa ctctaatcaa acatctcaat catccaacgg
    27541 tatgcctgat ttcgaccgtg atacttctga cccattcggc tcaagttcaa cgatcgacat
    27601 ttcagacgat gatttaccat tctaaacgaa tttgatagga gggaaataga atggcacaac
    27661 aaagaagagg cggacgtaaa cgtcgtaaag tagactacat tgctgctaac catattgaat
    27721 atatcgatta taaagatatc gaattactaa aaagatttat ttcagaacgc ggtaaaatct
    27781 tgccacgtcg tgtaactggt acaggcgcta aaaaccaacg taaaattact gttgcaatca
    27841 aacgcgctcg tatcatgggc ttgctacctt tcgttagcga agactaataa atcctaggaa
    27901 ttgaatgact gatccttttg atcagctatt caattccttt ttttctgtaa aaataacctc
    27961 tattcgttta gtcgctcgaa tgacgaaagt tatggtaaaa tcaaattagt catatatggg
    28021 aggaaaatta tggaaaataa aaacaatcat ttatccccga aacctctctt ccttatcctg
    28081 tttatattaa tcgaagtatt ggtagtctta attttaccat ttcgttttgt tagcgcttcg
    28141 gtcattctat tgatgaattt atggggtgtt cgctattggt tgaaaatgaa acgttggctt
    28201 attttaaata aacgtgaaaa aatccagcag gcttcgactg tagctgaaac caatctagct
    28261 tatgtctcag aagtcatgcc tgtaggagtg attgcttatc agccatcaag ccaaaaaatc
    28321 gaatggctca atccattagc attgaaaatc aaagatcaaa gtggattaac agaggaagaa
    28381 ttgctagata ctttttttac tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat
    28441 aaaacatttc agtttgatat ggatacagta aagggtgttg tttattttaa tgatattacc
    28501 caaaaccagt tgttaaaact ccatcagacg aacagtcagc cggtgattgg tattttatct
    28561 attgataact atgcagatgc cattgataaa ttagatgaca aagagatttc ttatttgaac
    28621 tctttcgtca ctacactagt ttctgactgg atgaatgaat accatgtctt tttcaaaaga
    28681 atcaacgcag aacggttttt cttcgtagca cagatgatcg atctgaaaaa gatgatggat
    28741 aaaaaatttg atttgctgga tcgctttcga gcagaagctg aaaaacagag ctcgcctatc
    28801 acccttagta tggggattgc ttatggctca agaaccctcg atgaaatcgg tggagaagct
    28861 cagaataatt tagatgttgc tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta
    28921 aaagaaggag caaaacctgt ttattatggg gggaaatctg ctaagaatgt caaacgcacg
    28981 cgtgttcgtt ctcgagcgat gagtaccgct ttgcgtcgga tttttgatga aacaggcgat
    29041 gtgtttatca tggggcacaa gtttccggat atggatgctt taggctctgc ttttggtgtc
    29101 agttgtctgg cacgcttcaa ccagaaaaaa gcttacatcg ttatcaatga gaacgagatc
    29161 attccagatg tagaacggtg tcttgaagaa atccataagc atcctgaatt agaaatgcaa
    29221 ctaatctctc cagaacgtgc catagagctg aaaaaagaag aggacctcct cgtgatggtg
    29281 gattaccata aaccttctct atcgatctct cagggcctat atgagcaatt tgataagatt
    29341 gtgatcattg atcaccatcg tcgtggggaa gagtttccaa gtaaacctct gctgacttat
    29401 attgaatctt cagcttcttc agcttcagag ctagtttctg agctgatcca ataccaaagc
    29461 agcaaaacaa atcgcttaga tcgttttgag gccacattat tgcttgcagg gatggttgta
    29521 gatacaaata gtttcaatgt ccgtacaact gcacgaactt ttgacgttgc cagctattta
    29581 aggtcttgtg gagcagacgc atccttagta caatatttat tgagctcgga tttagcgaca
    29641 tacttggaga tcagcaattt gattgctgga agcgactatg tgacttctga tattgttgtg
    29701 gcggcaggga cagacgaaaa agagtataat agtgttatag cagcaaaaac agcagataca
    29761 ttgctttcga tgatcaatat caatgcagct ttcgtcatta caaagcggac agacggcttg
    29821 attgggatca gtgccagaag taccggcacg atcaacgtcc aaatcatcat ggaatcctta
    29881 ggtggcggag gacacttcac aaacgcagct actcaactgg aaaatacgac agttgaggaa
    29941 acaaaacaga agctatttgc tgcaatcaaa gaaaatatga atcaaatata tgataaggag
    30001 taatccaaat gaaagtaatt tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca
    30061 tcaaagaagt tccagcaggt tatgcacaaa attatctaat caaaaataac ttggcaaaag
    30121 aagcaacgaa aagcagtatc agtcagttag caggacaaaa gaaagcacaa gaaaaacacg
    30181 aagctgaaat cttggaagaa gctaaaaagt taaaagaatt cttggaaaaa gaagatactg
    30241 tagtcgaatt gaaggcgaaa gcaggggaag acagccgttt gtttggttcg atcccttcaa
    30301 aacaaattgc tgaagggtta aacaaacaat acaaaatcaa attagataaa cgaaaaatcg
    30361 aattagaaaa tccgatccgt tcactaggct acacaaacgt acctgttaag cttcatcatg
    30421 aggtgacggc tacgatcaaa gttcatgtcg tacaagaata acaaatcttg atagcgatgt
    30481 tcagctggta agtataacca gaaggaaaaa agacaaaccg aaatttatct gagaaaacag
    30541 ctgcagcaat gaaagcaaaa gctgcaaatc gatttcagat tgatttcggt ttcttttttg
    30601 ggaacagcaa aatagcataa agaaaacgag tataataaaa gagaactatt tttttattac
    30661 actttcggta cgaatgatga cttttctctc attttcttgt aaaatgaagc aattgaagac
    30721 aaggagcgat acgtatgaat gaagtgtggc aggatcgaat cccaccgcaa aatattgaag
    30781 ctgaacaagc ggttttaggc tctgtttttc ttgacgcaga aacgatcatt gatgccatgg
    30841 agtacataga gccaaaagac ttttatcgtc gtggccatca aattattttt gagacaatga
    30901 tcgagttaaa tgatcgtaat gaagcgattg acgtggttac tgtcaaagac cggctggaac
    30961 aagccaattt gttagaagat gccggtggtt taagttatct ctcagacttg gcattagcag
    31021 ttccgacagc agcaaatatc gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc
    31081 gtacattgat ccagacagca acaggaatcg ttacgaaagg attcgagcaa ggagaagaag
    31141 ttcaaacaat tttagatgat gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc
    31201 gaagcggctt tttatctatt gctgaagtac tgaactccac gattgaaaat atcgatcgac
    31261 tagctcaaaa caatgaagaa atcacaggtc taccaactgg ctatcaaggt ctggataaaa
    31321 tgacagcagg tttccagccc gaagaactaa tcattcttgc tgcacgtcca gcggtaggta
    31381 aaacagcgtt tgcattgaat attgctcaaa atgtaggaac aaaaacagat cgttctgttg
    31441 cgatatttag tttggaaatg ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag
    31501 gttcgattga ggccagccat ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc
    31561 tgatcgttgc gatggggagt ctttctcgag caaatatttt tatcgatgat acaccaggga
    31621 tcaaaatctc tgaaatccgt gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag
    31681 gcttgatatt gatcgattat ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac
    31741 aagaagtatc agatatctct cgccaactga aaaaactggc gaaagagctg aaagtacctg
    31801 tcattgctct ttcacagtta tctcgtggag tagaacagcg acaagacaaa cgaccagtgc
    31861 taagtgatat tcgtgaatcc ggatcaattg aacaagatgc ggacatcgta gccttcttgt
    31921 atcgagatga ttattatgaa agagaaggcg gagatgacga tgatggaaac gagccaaaaa
    31981 atgacaacgt catcgaagtc atcatcgaga agaaccgaag cggtgcacga ggaacagttg
    32041 aactactgtt tatcaaagaa tacaataagt tttcgtcgct ttcgccaaga gaagaatttt
    32101 aaagaagagg ttgtgagcct tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt
    32161 aaccgcagga acgactagag tacttaattt aaacagatct ccaaagggat tgtgacaaaa
    32221 gtcttgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgacc cttggtatta
    32281 gctcagcaaa cgtcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt
    32341 caaaacgctg atcttacgac ctcttttcct taaacggtgt tctcctgcca actcctcggt
    32401 cacaagtcac aatatcatca aacatgagaa gcagtttttt gaaattgctc cttgtgactc
    32461 agagttaaac ggcaggctca caacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    32521 ggtaatacgc ccaatcaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatt
    32581 acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaaa agtagcttct
    32641 tcgaaaaata agtcgaaatt tcacaaaaat ttgagaagca attttcgtac attccttctt
    32701 atttcttgaa gctgaccact tctgttacaa cctctttttt aataaccatt ctaaaaagat
    32761 aaagttcgtt ttttaagcgt ttttataaaa taaaaataaa aatcattcga ttttatttga
    32821 aatatgtatc tattattgat acaatggttc aggattaaat aatatagaac gaggtgttca
    32881 catgtcatct gtagtagtag taggtacgca atggggcgat gaagggaaag gaaaaatcac
    32941 agactttctc agtgaaaatg ctgaagtgat tgcacggtac caaggtggcg acaatgcagg
    33001 gcacacaatt aagtttaacg gtgtgactta taaactgcat ttgattcctt ctgggatttt
    33061 ttataaagac aagatcagtg tgatcggaaa cggcgttgta gtcaatccta aatcattggt
    33121 taaagaatta gcttatttac atgaaaaagg tgtagaaaca agtaatttgc gtatttctga
    33181 ccgcgcgcac gtaatcttgc cttatcatat caaattggac caattacaag aagatgctaa
    33241 gggcgaaaat aagattggta caacgattaa aggaatcgga cctgcctata tggataaagc
    33301 agctcgtgta ggtatccgta ttgcagactt attagataaa gaaatttttg aagaacgtct
    33361 tcgtttgaat ctagatgaca aaaaccgtca atttgtaaaa atgtttgatt cagaaccact
    33421 tgattttgac gatatttttg aagaatatta tgaatacgga caacaaatca aacaatacgt
    33481 cacagatact tccgttatct tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga
    33541 aggcgcacaa ggtgtaatgc tggatatcga ccagggaact tatccttttg ttacttcttc
    33601 taacccagta gccggtgggg taacaatcgg aagcggtgta gggccttcaa aaatcaataa
    33661 agttgtcggc gtctgcaaag cctatacgtc acgtgtcggt gatggaccat ttccaactga
    33721 attatttgat gaaacaggag aacaaatccg cgaagtagga cgagaatacg gtacgacaac
    33781 tggtcgtccg cgtcgtgtag gctggtttga ttctgtagtt atgcgccatt ctaaacgagt
    33841 ttcaggtatt accaacctat ctttaaactc gatcgatgta ttaagtggat tggaaacagt
    33901 aaaaatttgt acggcttatg aattagatgg cgaactaatc taccactatc cagcaagctt
    33961 gaaagagttg aaccgctgca aaccaatcta tgaagaattg ccaggctggt cagaagacat
    34021 tacaggatgc aaaacacttg cagaactgcc agaaaacgca cgtaactatg tacgtcgtat
    34081 ctcagagctt gttggtgtac gtatctcaac attttctgtc ggacctgata gaacacaaac
    34141 aaacatctta gaaagcgtct gggcgcaaat ctaaggataa aaacgacgac tgaatcagga
    34201 cgttcttacc agagcgagtc gaagtgttta tctatacgat aataataact tgaagaggta
    34261 gtgaaagaga cgtttagcct tattgccgat aggctgtctc ttgaacactg tttattcaga
    34321 ataagggact gtgacaaaaa ccgtgtcaca gtcccttttt ccgaa
//
LOCUS       DAZKCE010000016        33508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000016 DAZKCE010000000
VERSION     DAZKCE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.016"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..615)
                     /locus_tag="ACG8KQ_000698"
                     /pseudo
     CDS             complement(1..615)
                     /locus_tag="ACG8KQ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            763..1905
                     /locus_tag="ACG8KQ_000699"
     CDS             763..1905
                     /locus_tag="ACG8KQ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9103666.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEETETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPTLSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHLTPYSQRQTIEQDSAID
                     SGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNKSKPEIT
                     PGEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQ
                     GIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTF
                     ASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(2031..3110)
                     /locus_tag="ACG8KQ_000700"
     CDS             complement(2031..3110)
                     /locus_tag="ACG8KQ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9103667.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3219..3659)
                     /locus_tag="ACG8KQ_000701"
     CDS             complement(3219..3659)
                     /locus_tag="ACG8KQ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9103668.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3716..4126)
                     /locus_tag="ACG8KQ_000702"
     CDS             complement(3716..4126)
                     /locus_tag="ACG8KQ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9103669.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4307..4741)
                     /locus_tag="ACG8KQ_000703"
     CDS             complement(4307..4741)
                     /locus_tag="ACG8KQ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9103670.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4841..5689)
                     /locus_tag="ACG8KQ_000704"
     CDS             complement(4841..5689)
                     /locus_tag="ACG8KQ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9103671.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6078..7844
                     /locus_tag="ACG8KQ_000705"
     CDS             6078..7844
                     /locus_tag="ACG8KQ_000705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9103672.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7946..9205
                     /locus_tag="ACG8KQ_000706"
     CDS             7946..9205
                     /locus_tag="ACG8KQ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9103673.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9352..10653
                     /locus_tag="ACG8KQ_000707"
     CDS             9352..10653
                     /locus_tag="ACG8KQ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103674.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10656..11507
                     /locus_tag="ACG8KQ_000708"
     CDS             10656..11507
                     /locus_tag="ACG8KQ_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9103675.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11520..12335
                     /locus_tag="ACG8KQ_000709"
     CDS             11520..12335
                     /locus_tag="ACG8KQ_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9103676.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12486..13652
                     /locus_tag="ACG8KQ_000710"
     CDS             12486..13652
                     /locus_tag="ACG8KQ_000710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9103677.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13882..14529
                     /locus_tag="ACG8KQ_000711"
     CDS             13882..14529
                     /locus_tag="ACG8KQ_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9103678.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14613..15557
                     /locus_tag="ACG8KQ_000712"
     CDS             14613..15557
                     /locus_tag="ACG8KQ_000712"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9103679.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15692..17143
                     /locus_tag="ACG8KQ_000713"
     CDS             15692..17143
                     /locus_tag="ACG8KQ_000713"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9103680.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17164..18000
                     /locus_tag="ACG8KQ_000714"
     CDS             17164..18000
                     /locus_tag="ACG8KQ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9103681.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18000..18659
                     /locus_tag="ACG8KQ_000715"
     CDS             18000..18659
                     /locus_tag="ACG8KQ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9103682.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18785..19474
                     /locus_tag="ACG8KQ_000716"
     CDS             18785..19474
                     /locus_tag="ACG8KQ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103683.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19480..20436
                     /locus_tag="ACG8KQ_000717"
     CDS             19480..20436
                     /locus_tag="ACG8KQ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9103684.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20480..20992
                     /locus_tag="ACG8KQ_000718"
     CDS             20480..20992
                     /locus_tag="ACG8KQ_000718"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9103685.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21248..21796)
                     /locus_tag="ACG8KQ_000719"
     CDS             complement(21248..21796)
                     /locus_tag="ACG8KQ_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9103686.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21885..23066)
                     /locus_tag="ACG8KQ_000720"
     CDS             complement(21885..23066)
                     /locus_tag="ACG8KQ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9103687.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23302..24810)
                     /locus_tag="ACG8KQ_000721"
     CDS             complement(23302..24810)
                     /locus_tag="ACG8KQ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9103688.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24969..25160
                     /locus_tag="ACG8KQ_000722"
     CDS             24969..25160
                     /locus_tag="ACG8KQ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103689.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25363..26280
                     /locus_tag="ACG8KQ_000723"
     CDS             25363..26280
                     /locus_tag="ACG8KQ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9103690.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26273..27346
                     /locus_tag="ACG8KQ_000724"
     CDS             26273..27346
                     /locus_tag="ACG8KQ_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103691.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27494..28012
                     /locus_tag="ACG8KQ_000725"
     CDS             27494..28012
                     /locus_tag="ACG8KQ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103692.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27993..28385
                     /locus_tag="ACG8KQ_000726"
     CDS             27993..28385
                     /locus_tag="ACG8KQ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9103693.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28461..29396
                     /locus_tag="ACG8KQ_000727"
     CDS             28461..29396
                     /locus_tag="ACG8KQ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9103694.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29529..30308
                     /locus_tag="ACG8KQ_000728"
     CDS             29529..30308
                     /locus_tag="ACG8KQ_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9103695.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30301..30618
                     /locus_tag="ACG8KQ_000729"
     CDS             30301..30618
                     /locus_tag="ACG8KQ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9103696.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30862..32472
                     /locus_tag="ACG8KQ_000730"
     CDS             30862..32472
                     /locus_tag="ACG8KQ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9103697.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32629..33477
                     /locus_tag="ACG8KQ_000731"
     CDS             32629..33477
                     /locus_tag="ACG8KQ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9103698.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11085 a         5976 c         6510 g         9937 t
ORIGIN      
        1 ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt catcataaaa
       61 ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat attgaatttg
      121 tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag acaaaccgta
      181 ctatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg cactatagtt
      241 tgtttgctga ctgaaactga taatcgaatt tgtctgacag ctgaacaaag aagtcaagat
      301 ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc agtaagccgg
      361 atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat gcagttctgt
      421 tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa aatttgattc
      481 agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat gttcagatag
      541 gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg ttttaatcgc
      601 ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact tgaaaatatt
      661 actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga atctctacta
      721 tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata ttcaaccatc
      781 ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac agtccacaca
      841 gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa tcgaattacc
      901 tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa aggacaaaca
      961 gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact acaatcagct
     1021 gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa cactttgact
     1081 atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga cttgttaaaa
     1141 ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca tttaacgccg
     1201 tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg agatcttgtt
     1261 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
     1321 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
     1381 ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc agatgtaact
     1441 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
     1501 aatgaaactg aaaatccaga aaaactagag gtagatccag aagaaaaacc agatgtgact
     1561 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
     1621 attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat
     1681 gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta
     1741 aaaagtagca aggatacaac ctttgctagt cctgaaacga aaaataaaca gctaccaaaa
     1801 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1861 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1921 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa cgaccccaaa ataccagatt
     1981 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     2041 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     2101 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     2161 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     2221 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     2281 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     2341 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     2401 ccaaatacag ccaaggcgat tgtagtgagc aacgtgttta aaagagcgat aacaaattga
     2461 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     2521 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     2581 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     2641 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     2701 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2761 tcgtaaaaat caataacgga ctggatcata tcaaatgttt gatgaataag catcgggata
     2821 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2881 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2941 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     3001 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     3061 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     3121 aatatgaaaa taagaaagag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
     3181 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
     3241 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
     3301 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
     3361 ccttgaggag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
     3421 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
     3481 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
     3541 agatactggg tataggtaag attcagcggc ttgaggaggg aagtgtagtg tctaatgatt
     3601 tccttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
     3661 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
     3721 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
     3781 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
     3841 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
     3901 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
     3961 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
     4021 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatga acttctccag
     4081 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa ttaccacctt
     4141 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
     4201 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
     4261 atatacaaaa aaatatcaga gacaaaaact aatctcatca taaacattac atatatttct
     4321 caaaaagttg ttttctttct tcaaaactta cttcacgatg gacctgatga agcatccata
     4381 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
     4441 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
     4501 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
     4561 gagacgtgtt ttcatccagc atatggaatc tcgtgccata caaggtaaaa atgacttcat
     4621 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
     4681 acagttctag tgctttcaaa ctatcattta atacaaaatc tatttccgtt cctttatgca
     4741 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagttaa
     4801 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
     4861 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
     4921 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
     4981 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
     5041 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
     5101 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
     5161 ataaaatagc ataaagaaac tcttctattc caaaaacaaa cagattaaga gaaacaacga
     5221 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
     5281 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
     5341 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
     5401 ctggaactat tgcaatgatt aaaggaaaaa acaagcttcc gaaaagtact ttaaaaaagg
     5461 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
     5521 ctatagctcg gtcaaatcct aaaacagctt ccagtaatat tcctagaccg ctgacacctc
     5581 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
     5641 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt tatccacttc
     5701 cttaattcat taataatact cttatcttat tataacttac ttttcacctt aagttaactt
     5761 taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct agaatatact
     5821 tctacgttaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
     5881 agataaattc aggacactaa atagtacagg aagtagatta aaacttttaa aagttttaag
     5941 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
     6001 catttttgaa agcgttgtct tttgtttctc tatcacttat agtgatgacg tcatattaaa
     6061 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
     6121 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
     6181 aaaaagtata tgtgctttgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
     6241 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
     6301 tgacgagcga aacaaggcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
     6361 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
     6421 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
     6481 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
     6541 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gacttcttcg
     6601 ggggagattt gcaaggtgtg agggatcatt tggattactt gacggacctt ggcgtcaatg
     6661 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
     6721 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
     6781 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
     6841 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
     6901 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
     6961 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
     7021 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
     7081 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
     7141 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
     7201 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattc acagattcga
     7261 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
     7321 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
     7381 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
     7441 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
     7501 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
     7561 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
     7621 ttatttctga aggttctact cagtggctaa tagtatatga tcgtgaagat aagctctatt
     7681 tcacacggga actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
     7741 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
     7801 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
     7861 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
     7921 gttgaataca caggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
     7981 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
     8041 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
     8101 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
     8161 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
     8221 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
     8281 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
     8341 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
     8401 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
     8461 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
     8521 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
     8581 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
     8641 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
     8701 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
     8761 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
     8821 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
     8881 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
     8941 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
     9001 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
     9061 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
     9121 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
     9181 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
     9241 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
     9301 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
     9361 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
     9421 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
     9481 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
     9541 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
     9601 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
     9661 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
     9721 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
     9781 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
     9841 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
     9901 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
     9961 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
    10021 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
    10081 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
    10141 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
    10201 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
    10261 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
    10321 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
    10381 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
    10441 atgatctatc tgtttaacaa tggtggtccc ggaagcgtag gcggaggagc tggttctact
    10501 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
    10561 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
    10621 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
    10681 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
    10741 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
    10801 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
    10861 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
    10921 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
    10981 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
    11041 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
    11101 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
    11161 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
    11221 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
    11281 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
    11341 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
    11401 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
    11461 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
    11521 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
    11581 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
    11641 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
    11701 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
    11761 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
    11821 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
    11881 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
    11941 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
    12001 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
    12061 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
    12121 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
    12181 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
    12241 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
    12301 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
    12361 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
    12421 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
    12481 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
    12541 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
    12601 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcttctt
    12661 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
    12721 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
    12781 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
    12841 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
    12901 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
    12961 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
    13021 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
    13081 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
    13141 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
    13201 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
    13261 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
    13321 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
    13381 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
    13441 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
    13501 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
    13561 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
    13621 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
    13681 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
    13741 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
    13801 taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt
    13861 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
    13921 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
    13981 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
    14041 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
    14101 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
    14161 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
    14221 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
    14281 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
    14341 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
    14401 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
    14461 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
    14521 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
    14581 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
    14641 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
    14701 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
    14761 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
    14821 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
    14881 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
    14941 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
    15001 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
    15061 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
    15121 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
    15181 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
    15241 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
    15301 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
    15361 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
    15421 tacagattgg tacagagctt cataaagaag ggccggaaat tttttctaga atcataaaag
    15481 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
    15541 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
    15601 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
    15661 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
    15721 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
    15781 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
    15841 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
    15901 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
    15961 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
    16021 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
    16081 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
    16141 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
    16201 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
    16261 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
    16321 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
    16381 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
    16441 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
    16501 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
    16561 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
    16621 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
    16681 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
    16741 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
    16801 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
    16861 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
    16921 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
    16981 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
    17041 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
    17101 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
    17161 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
    17221 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
    17281 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
    17341 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
    17401 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
    17461 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
    17521 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
    17581 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
    17641 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
    17701 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
    17761 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
    17821 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
    17881 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
    17941 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
    18001 tgtactttcc cctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
    18061 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ttggtagggt
    18121 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
    18181 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tatttcgggt
    18241 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
    18301 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
    18361 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
    18421 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
    18481 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
    18541 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
    18601 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag tcaatctgaa
    18661 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
    18721 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
    18781 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
    18841 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
    18901 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
    18961 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
    19021 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
    19081 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
    19141 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
    19201 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
    19261 ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
    19321 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
    19381 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
    19441 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
    19501 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
    19561 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
    19621 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
    19681 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
    19741 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
    19801 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
    19861 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
    19921 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
    19981 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
    20041 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
    20101 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
    20161 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
    20221 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
    20281 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
    20341 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
    20401 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
    20461 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
    20521 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
    20581 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
    20641 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag gcgtggatcc
    20701 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
    20761 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
    20821 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
    20881 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
    20941 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
    21001 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
    21061 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
    21121 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
    21181 tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg agtattattc
    21241 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
    21301 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
    21361 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
    21421 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
    21481 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
    21541 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
    21601 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
    21661 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
    21721 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
    21781 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
    21841 aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac cagcaagcca
    21901 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
    21961 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
    22021 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
    22081 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
    22141 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
    22201 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
    22261 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
    22321 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
    22381 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
    22441 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
    22501 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
    22561 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
    22621 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
    22681 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
    22741 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
    22801 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
    22861 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
    22921 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
    22981 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
    23041 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
    23101 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
    23161 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
    23221 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
    23281 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
    23341 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
    23401 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
    23461 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
    23521 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
    23581 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
    23641 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
    23701 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
    23761 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
    23821 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    23881 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    23941 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    24001 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    24061 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    24121 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    24181 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    24241 cagttggttc aagcaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    24301 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    24361 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    24421 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    24481 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    24541 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    24601 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    24661 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact aacttcccac caaattctaa
    24721 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    24781 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    24841 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    24901 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    24961 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    25021 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    25081 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    25141 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    25201 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    25261 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    25321 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    25381 aatgcaaatt atgagccaga cgaactagag gagacgaacg cggatcagga tacaggacaa
    25441 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    25501 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    25561 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    25621 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    25681 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    25741 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    25801 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    25861 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    25921 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    25981 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    26041 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    26101 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    26161 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    26221 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    26281 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    26341 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    26401 agaggaaaca aaaatacatg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    26461 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    26521 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    26581 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    26641 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    26701 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    26761 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    26821 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    26881 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    26941 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    27001 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    27061 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    27121 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    27181 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    27241 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    27301 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    27361 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    27421 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    27481 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    27541 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    27601 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    27661 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    27721 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    27781 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    27841 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    27901 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    27961 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    28021 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    28081 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    28141 gtcaaaactt ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta ctgagtattt
    28201 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    28261 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    28321 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    28381 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    28441 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    28501 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    28561 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    28621 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    28681 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    28741 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    28801 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    28861 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    28921 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    28981 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    29041 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    29101 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    29161 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    29221 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    29281 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    29341 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    29401 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    29461 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    29521 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    29581 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    29641 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    29701 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    29761 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    29821 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    29881 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    29941 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    30001 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    30061 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    30121 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    30181 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    30241 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    30301 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    30361 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    30421 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    30481 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    30541 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    30601 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    30661 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    30721 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    30781 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    30841 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    30901 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    30961 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    31021 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    31081 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    31141 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    31201 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    31261 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    31321 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    31381 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    31441 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    31501 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    31561 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    31621 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    31681 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    31741 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    31801 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    31861 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    31921 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    31981 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    32041 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    32101 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    32161 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    32221 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    32281 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    32341 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    32401 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    32461 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    32521 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    32581 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    32641 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    32701 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    32761 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    32821 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    32881 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    32941 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    33001 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    33061 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
    33121 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    33181 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    33241 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    33301 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    33361 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    33421 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    33481 cttttattca aaaaacatac caaaaata
//
LOCUS       DAZKCE010000017        33366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000017 DAZKCE010000000
VERSION     DAZKCE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.017"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..505
                     /locus_tag="ACG8KQ_000732"
     CDS             2..505
                     /locus_tag="ACG8KQ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103699.1"
                     /translation="MNFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDS
                     GLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNR
                     KIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNP
                     KTYDILA"
     gene            complement(566..931)
                     /locus_tag="ACG8KQ_000733"
     CDS             complement(566..931)
                     /locus_tag="ACG8KQ_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9103700.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1007..1252)
                     /locus_tag="ACG8KQ_000734"
     CDS             complement(1007..1252)
                     /locus_tag="ACG8KQ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9103701.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1322..1576)
                     /locus_tag="ACG8KQ_000735"
     CDS             complement(1322..1576)
                     /locus_tag="ACG8KQ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103702.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1740..2063)
                     /locus_tag="ACG8KQ_000736"
     CDS             complement(1740..2063)
                     /locus_tag="ACG8KQ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103703.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2250..2323
                     /locus_tag="ACG8KQ_000737"
     tRNA            2250..2323
                     /locus_tag="ACG8KQ_000737"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2284..2286,aa:Ile,seq:cat)
     gene            complement(2708..3205)
                     /locus_tag="ACG8KQ_000738"
     CDS             complement(2708..3205)
                     /locus_tag="ACG8KQ_000738"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9103704.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3245..4054)
                     /locus_tag="ACG8KQ_000739"
     CDS             complement(3245..4054)
                     /locus_tag="ACG8KQ_000739"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9103705.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4388..5245
                     /locus_tag="ACG8KQ_000740"
     CDS             4388..5245
                     /locus_tag="ACG8KQ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9103706.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5238..6140
                     /locus_tag="ACG8KQ_000741"
     CDS             5238..6140
                     /locus_tag="ACG8KQ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9103707.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6173..6523
                     /locus_tag="ACG8KQ_000742"
     CDS             6173..6523
                     /locus_tag="ACG8KQ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9103708.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6572..7495
                     /locus_tag="ACG8KQ_000743"
     CDS             6572..7495
                     /locus_tag="ACG8KQ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9103709.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7573..8466
                     /locus_tag="ACG8KQ_000744"
     CDS             7573..8466
                     /locus_tag="ACG8KQ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9103710.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8504..9067
                     /gene="amaP"
                     /locus_tag="ACG8KQ_000745"
     CDS             8504..9067
                     /gene="amaP"
                     /locus_tag="ACG8KQ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9103711.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9081..9269
                     /locus_tag="ACG8KQ_000746"
     CDS             9081..9269
                     /locus_tag="ACG8KQ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9103712.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9282..10184
                     /locus_tag="ACG8KQ_000747"
     CDS             9282..10184
                     /locus_tag="ACG8KQ_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9103713.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10216..10458
                     /locus_tag="ACG8KQ_000748"
     CDS             10216..10458
                     /locus_tag="ACG8KQ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9103714.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10714..11085)
                     /locus_tag="ACG8KQ_000749"
     CDS             complement(10714..11085)
                     /locus_tag="ACG8KQ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103715.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(11827..12906)
                     /locus_tag="ACG8KQ_000750"
     CDS             complement(11827..12906)
                     /locus_tag="ACG8KQ_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9103716.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            13093..13791
                     /locus_tag="ACG8KQ_000751"
     CDS             13093..13791
                     /locus_tag="ACG8KQ_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9103717.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(13959..16598)
                     /locus_tag="ACG8KQ_000752"
     CDS             complement(13959..16598)
                     /locus_tag="ACG8KQ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9103718.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(17217..18248)
                     /gene="queA"
                     /locus_tag="ACG8KQ_000753"
     CDS             complement(17217..18248)
                     /gene="queA"
                     /locus_tag="ACG8KQ_000753"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9103719.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            18441..19615
                     /locus_tag="ACG8KQ_000754"
                     /pseudo
     CDS             18441..19615
                     /locus_tag="ACG8KQ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            19619..20260
                     /locus_tag="ACG8KQ_000755"
     CDS             19619..20260
                     /locus_tag="ACG8KQ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103720.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            20257..21174
                     /locus_tag="ACG8KQ_000756"
                     /pseudo
     CDS             20257..21174
                     /locus_tag="ACG8KQ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            21171..21842
                     /locus_tag="ACG8KQ_000757"
     CDS             21171..21842
                     /locus_tag="ACG8KQ_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103721.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(22383..22871)
                     /locus_tag="ACG8KQ_000758"
     CDS             complement(22383..22871)
                     /locus_tag="ACG8KQ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9103722.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(23072..23338)
                     /locus_tag="ACG8KQ_000759"
     CDS             complement(23072..23338)
                     /locus_tag="ACG8KQ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9103723.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(23338..23601)
                     /locus_tag="ACG8KQ_000760"
     CDS             complement(23338..23601)
                     /locus_tag="ACG8KQ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9103724.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            24122..24262
                     /locus_tag="ACG8KQ_000761"
                     /pseudo
     CDS             24122..24262
                     /locus_tag="ACG8KQ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(24404..26227)
                     /locus_tag="ACG8KQ_000762"
     CDS             complement(24404..26227)
                     /locus_tag="ACG8KQ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9103725.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(26825..29530)
                     /locus_tag="ACG8KQ_000763"
     CDS             complement(26825..29530)
                     /locus_tag="ACG8KQ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9103726.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(29836..30783)
                     /locus_tag="ACG8KQ_000764"
     CDS             complement(29836..30783)
                     /locus_tag="ACG8KQ_000764"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9103727.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            30973..31242
                     /gene="rpsN"
                     /locus_tag="ACG8KQ_000765"
     CDS             30973..31242
                     /gene="rpsN"
                     /locus_tag="ACG8KQ_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9103728.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(31418..31780)
                     /locus_tag="ACG8KQ_000766"
     CDS             complement(31418..31780)
                     /locus_tag="ACG8KQ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9103729.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(31791..32912)
                     /gene="alr"
                     /locus_tag="ACG8KQ_000767"
     CDS             complement(31791..32912)
                     /gene="alr"
                     /locus_tag="ACG8KQ_000767"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9103730.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(32928..33278)
                     /gene="acpS"
                     /locus_tag="ACG8KQ_000768"
     CDS             complement(32928..33278)
                     /gene="acpS"
                     /locus_tag="ACG8KQ_000768"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9103731.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT        10194 a         6706 c         6393 g        10073 t
ORIGIN      
        1 tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct attttcaggg
       61 ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag caatggcagg
      121 ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag ccacgacaga
      181 ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac gatgcatggg
      241 aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag
      301 caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat cgtatacaga
      361 cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt ctagggaagt
      421 tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg aagataaccc
      481 taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat gaagtaagag
      541 gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc tccaaatttg
      601 ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg ttgcgctcta
      661 cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga tttttccact
      721 ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga tgatattgga
      781 ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc tttccccagc
      841 catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga cgaggaagct
      901 ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat atagattgtt
      961 aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc
     1021 ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag tcagcgatat
     1081 agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga tatttgaata
     1141 aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca gttcttaaga
     1201 aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc atttaatcac
     1261 ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca atcaatagta
     1321 ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa gacaaaacca
     1381 gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt
     1441 tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt tctttgttcc
     1501 aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc
     1561 tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag ataacatgtt
     1621 attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat
     1681 gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc
     1741 tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg
     1801 atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca
     1861 aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga
     1921 agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc tgaggtttga
     1981 taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat cgcaataaat
     2041 ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt ttctaaaata
     2101 agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa taagaataac
     2161 aatattattt tacttatatt ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa
     2221 aagtaacaaa aataaatttt atttatgatg tacctttagc tcagttggtt agagcagacg
     2281 gctcataacc gtccggtcgt aggttcgagt cctacaaggt acataagagg ttgtgaaaaa
     2341 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt
     2401 ggctgttctt attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa
     2461 atcagcgttt tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc
     2521 cgtttgttaa gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg
     2581 agcctgccgt ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt
     2641 aggactaaaa cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata
     2701 gatattttta tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt
     2761 ctccgtgttt tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag
     2821 cactctgcag gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt
     2881 cgatctcttt ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt
     2941 caggtggaat cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggagaaagaa
     3001 gagggatcaa ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat
     3061 cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac
     3121 gttctgccaa ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat
     3181 tttgcatatc aataataact aacattttct ttcctcctac attcatatcg tcattttatt
     3241 ctttttaata gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta
     3301 cttggtttgc aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc
     3361 tgtaattcaa catagcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt
     3421 cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg
     3481 tgatttctaa gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc
     3541 tttgtagctt ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa
     3601 gagaaggatc ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat
     3661 gttcataata agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt
     3721 gggtttctcg gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc
     3781 tcagtgtatc gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca
     3841 tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa
     3901 aaatatcttt agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt
     3961 catcaaattt ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca
     4021 aggtaccgtc taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat
     4081 atctattttc ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct
     4141 ttgtcattat gaattttttc aaaaaacatt cttaagaaaa aggtggctat atatagtagt
     4201 ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg
     4261 cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta
     4321 aaaccaaatg aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt
     4381 gattttgatg gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta
     4441 tgctccttac ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg
     4501 cataaaattt tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga
     4561 aacggtaaat aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat
     4621 ctacgaagac ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt
     4681 accaaactta ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt
     4741 agtactagac gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc
     4801 tattatcgat ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt
     4861 gtacttcaat tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac
     4921 agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga
     4981 tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca
     5041 gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa
     5101 agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac
     5161 gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac
     5221 gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa
     5281 aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta
     5341 aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac
     5401 gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag
     5461 aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg
     5521 cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca
     5581 tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag
     5641 cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat
     5701 caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa
     5761 atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg
     5821 accaagatgc attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga
     5881 atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg
     5941 aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt
     6001 ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg
     6061 ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa
     6121 cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag gaatggaaat
     6181 tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat
     6241 cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa
     6301 aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat
     6361 cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg
     6421 attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt
     6481 actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc
     6541 aaaaaggagg tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac
     6601 agatgaaaaa aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca
     6661 agcaatcgaa gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga
     6721 agtttctcgg atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt
     6781 gattaatcta gatcagtcag ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt
     6841 actttccgat aatctcttga ttacttgtat cgatagaaaa accgaatttg tggatcggct
     6901 gctgacaaag catggaaatc atttaaactc gtttgaaaag atcatcacgt atacattatt
     6961 agatatttac acccattacg tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt
     7021 ggataaagca gctcgtaaaa caacggaaaa tgaagaactg tataaacagg cagatcttga
     7081 aagagacatc gtatatatcg atcatacctt aagagatcaa cgggaaacaa tggatcaatt
     7141 atggaattct gaaactttca tcaaacgttt agacgatgaa cagctactct atgatgtcca
     7201 tttgagacag cgacaaacag aaaaaatgat cgagatctat cgagatttat tagagagtat
     7261 tggtgatcta ttcaacggga tgatggacaa taatctaaat catatcatga aatatctaga
     7321 ttcagctgca ttaatcattt ccgttccggc gttgttcgca gggatttggg gaatgaatac
     7381 aggaggatta ccgggtaaaa gttctgctct aggctttata ctcgtagtct ttggctcttt
     7441 gatagcagca gtagttttag gttaccattt ataccggaaa gattatacaa attaaagaat
     7501 ctatttaaaa caggtatcaa acaaagaaat ttgttttggc aatacaattt aaaaactagg
     7561 aggaatacag caatgacaga accaaaatta atcgacccgc gaaacttgta ccatacggag
     7621 aaatttccta aacaagatca ggatacacca gctttacagc agaatatgat tcctgttcct
     7681 gattgtggag aagaatccta tgaaggcaat aatcaactag aaaaccgccg tgtactaatc
     7741 actggtggag attcagggat cggtcgtgca gcagcgattg catttgctag agaaggggca
     7801 gatatcgctc ttcaattctt cccaggagaa gaaaaagatg cagaggaagt agcaaaatac
     7861 gtgaaagttg caggacgtaa gattgtctta ctaccttttg atcttcgtga cgaacaagca
     7921 cctaaagaaa tcatagataa agccgtgagt gaacttggcg ggctagatac gatggtacta
     7981 aacgcaggac agcaaatttc ttgtcagtct attgctgagt taccacttga gcaagtcaaa
     8041 gacacattta tgataaacat catcgctatg tttgctttag tgaaagaagc tgttccgcat
     8101 atcccagcag gaggagcaat cgtaacaaca acttctgttc aagcattcaa tccaagcgaa
     8161 catttattag attacgcagc aacaaaagct tcaatcgcta actttacgat cggtttagca
     8221 aaacagcttg cacctaaagg aatccgtgta aatggtgtag cacctggtcc aatctggaca
     8281 ccattacaac tagacgaagg acaaccttct gattcgatcc ctgaatttgg tcagcaaagt
     8341 ttactagaac gtgcaggtca gccaggagaa ttagcaccag tttatgtttt cttagcatcg
     8401 aataaagcaa gttatgttac agcccaaatt tacggagtaa ctggtggaga agcgatcaac
     8461 ttataagaag ttgatcgttt cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa
     8521 aacgatcgga gtgatcggaa cattactttt gctatctgtg cttttgtttg cgatactatt
     8581 aagtgcaacc tatccattgc cttttcagct agagaagttt cgattctttt ccatcactaa
     8641 ttcatacgtg caacaatatg tattttggct tgccgttgca tttgctattt tgacaattat
     8701 cacgttactt gttttcctgt tttatccaaa aacaatcgga aactttctac tgaaacatgg
     8761 tgatggggag ttgacgttgg ataaaaaagc aattgaagga atggttcgtt ctcacttgca
     8821 tgaagatgaa ttcatccatt caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc
     8881 cgtcaatgta aaaggggagt tgaaacgaac ttcagcactc attggtaaaa caggaacatt
     8941 gatgaaagaa atcgaaaatg aagtacaaca catattggga acaaaagagc cagtgaaagt
     9001 atcggtgaaa tacacaggct ttgaagaacc agctaaacga agcaatgacc gcaaacgagt
     9061 agaatagggg gggaccaaaa atgaaagaac tattttcgac atacaagctc cctatcatct
     9121 ttggggtact tggattgata ttagccgttt tatggatcac cgtgggcttt ttcaagacct
     9181 tattgatctt gatcatggca gcgatcggcg tcgctatcgg tttttatctg aaacaaacga
     9241 aaatcttaga tcactatttt aaatcttagg aggaaacatc catgaataac aataacaaaa
     9301 ataataacaa taaaatgaac aaccaaaaac cagtgtctgc acatccagac ccaacgacac
     9361 caaaccaatc aaatggtgtg aacgatgaaa agaacaaaac agcaccagta tctgcacatc
     9421 cagatccagt agtccctaac gttcaacaag gagtagaaga gaagaaacat gatgcagaaa
     9481 agcatgcacc tcatcatcca ggaactccag aatctaaagt tccagaacaa catgatgcaa
     9541 tcgatccttc taaacctgtg gaagagcaaa aacatccttc tgaccatgcg aaagcacaag
     9601 aaaaacttag ttctgaccac acaaacggac aaggaaaact tggttctgat catacaaata
     9661 accaaggaaa gcatgattct gaacaggaag attcaatgcg cggtgagctg aattatgaag
     9721 ataaagttgt gcaaaaaatc atcggaatcg caattgaaca agtagacggt ttattatctg
     9781 caaacggtgg tttcttctct aatgtggcag gaaaacttgt gaatacagat aatgtaacgg
     9841 ctgggatcga aacagaagtc ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg
     9901 aatatggcaa agatatcgaa aaaatctttg aagaaatgca agaagtaatc ggcaaagaag
     9961 taaaaaatat gacacattta gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa
    10021 caaaagaaga atttgaaaaa gatcaagaaa cggtgcaaga caaagtaact gacgcagcga
    10081 aaaaaaccgg tgaatttgct tcgaatcaaa cagataaagt aaaatcagca gctggaagcg
    10141 gcgcacaaaa agtaaaagaa aatacagaac cacgggttca gtaataagga agtaaaacat
    10201 taggaggaac gaaacatggg atttatttgg tcacttatcg tcggaggaat tatcggagca
    10261 attgcaggag caatcacgaa taaaggttct tcgatgggga ttattgcaaa tgtcgtagca
    10321 ggtttagttg gatcagcact tggtcaagca ttattaggtt cttggggacc aagtattgca
    10381 ggaatggcaa tcgtgccatc attgattggt gccatcattt tagtactaat cgtatcattc
    10441 tttgctcgaa aactgtaaaa agacttttaa aaagaggtcg tgaaaaagct gttcttcatc
    10501 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttgggcatat
    10561 tatcgaagat gcaagctttt gaacactgtt tatacggaat aagaggctgt gacaagactt
    10621 ttgtcacagc ctcttattta tgcgggcaaa ttcccaatta gatttttaaa agtttttcct
    10681 taaatgtgtc tttattgata cagagaagct tctttaataa aacaatgcct ccctaaattt
    10741 tcttgagata ggaaaggtga acatacaaaa taatacgacc actttgaaaa cccagatgat
    10801 ccacaaatgg atcgtatgat actgatgaag taaaaagcaa tggatcagaa actcaaagag
    10861 attgaaaaag acaaggaaga aataatatct cttttttact cttgtctgtt ttagccaata
    10921 atagctcgta aagtaatcta gacaaacaaa tagaagtaaa caaagtttcg taagagcagc
    10981 aatcgtatca tgttgctctg ctagacgatt gacgagcagg aaaaatgttg gtaatggaat
    11041 atagatcacg agtaatgaaa gaaaaaagta aacaataaat ggcatactat tcctcctaat
    11101 tgtgtgagtt cgccctcatt ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg
    11161 taacaaatgt tatctaatgt atttttaaat aaataatcat tattatcaaa ataatgttaa
    11221 aaataaaaaa gaattgtcca tcactgttat tcaaatcatt ttatcaaaac tacatagtaa
    11281 atgttaaaaa gcaacatgac ataatatata gaaggcttta cagtatatat gggagcttac
    11341 taaaaaagaa tggagtaaga gttaacacaa tgggccatta gctgaagttt taagcacttc
    11401 aaggtatgaa acagaatggc ctgtaatact acatgattat ttagactgct caaactgcta
    11461 tcagtctggt ggaatgaata aagtattata ctaaaagcag atccaactac aacgtaacag
    11521 gaaaccagtt gtatcaggaa aagtgatagt acatggagaa gaaaaggccc cctttgtatg
    11581 agtcagtaca tatcttattt atagataaaa cggtattcaa tcgaaataaa aaatctcgta
    11641 aatttcaagt ttaagttagc cacccatggt gacccggttg ctcgttacca ccaaccgtct
    11701 aatttttcta atcgagtggg cccttgacaa tgagctgagg catggtagtc tcccgactgg
    11761 ctggccccgg ccagctttcc agacacaagc catgccgagg gaggctcgtt gtcaagggcc
    11821 cacgtgtcaa gccgtcaacc gttcctgcct aaatgcctgg acgaacgtgt catgcggcgt
    11881 tgcatagccc aaaatctttc gcgggtaatc gttcatccaa cgctgaatcc gcaagatttg
    11941 gttttcgctc acgttgccga ttgggctgcc cttggggatg aaacggcgaa tcagcttgtg
    12001 ttggttttcg ctcgtcccgc gctcccagga agcatagggg tggctgaagt agatgtttgc
    12061 cacatcctgc aaggcatcgt gcagtcccgc gaactccgag ccgttgtccg aggtaaccgt
    12121 cttgaacagc tggccgaagt acgcaccagc ccgttctctc agctgtcgga ccgcttggtt
    12181 gaccgattcg gcatccttcc cggccagctt cagaatgacc tcgaagcgtg tctgccgttc
    12241 caccaacgtc agcagcacgg catccgactt ggtcttgttt cctacgaccg tgtcgacttc
    12301 ccaatggccg aaggttttcc gggtaccaac cgcctccggg cgctcttcaa tggatttccc
    12361 tagcacccgt ttattcgggc ggttgccatg acgggacgat ttcggtttgc gggacagttt
    12421 ttctagcagg tccatattct tcgttttcat gatgccacgg tcgatccact gatagagggt
    12481 ggtggtccca gggataatgg ccgggtcgaa caattcgtgt tttctggcaa agcccaccac
    12541 cacgtccggg gaccattttt ccaacagcat catgtcgtcc gcccattcaa tgaaggcgtc
    12601 cgtgtcggcc cataagggcc gtctgccgca gttcaagcgg ttcttctcgt aggccgcctg
    12661 cccggcatcg gcgtcgtaga cgtcatgata atagtcgtag accttcccgt tctgattctg
    12721 gcggttaagc tgagtaacgg tcccgcgttt gatttcgttg tttatggtct gcggcacgca
    12781 gcccaggaca tccgcaacct gacggttgga atagttttcc tttttcagga tggcgatttg
    12841 gcaacgttct gagtaggtta ggtgttttcc cttgcgtgaa actgtgttat catggttttg
    12901 cgtcatgtga attcatcctt gtctattgag agttgtggta acttcaatat aacatggaat
    12961 tcacgtggcg tttttttatt tccaaatggc taagttaatt ataaaatccg ccaaaaaaag
    13021 aaaaacatta tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag
    13081 gagggaaaaa tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa
    13141 ggacgttggg gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact
    13201 attcttctta ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg
    13261 agcgtctcag aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt
    13321 tccagcatca ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta
    13381 cgtggacaaa aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg
    13441 tttatcggcg gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt
    13501 ttgctattag ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt
    13561 tactacgata tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca
    13621 agtcgaaaat tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc
    13681 ggctggcata tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac
    13741 atttctgcaa caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg
    13801 tgacttgtga ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac
    13861 attcagccca ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat
    13921 agttccagta aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc
    13981 ggtaatcgat ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct
    14041 accaagaacc agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc
    14101 agcaaagcaa cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta
    14161 ttggtcgcta aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga
    14221 atcgatgaat aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct
    14281 acccatgggc tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg
    14341 gtgaagaccc ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca
    14401 tttttcggaa tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca
    14461 aatcctggaa gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt
    14521 gctccccatc ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt
    14581 agcaaaaagt tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg
    14641 tctacgatcg tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca
    14701 tctgttcctg tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca
    14761 ttcacaccat ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc
    14821 cggatcttgt cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct
    14881 aattcttcgt ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc
    14941 aagataccta attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg
    15001 actgttttga tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt
    15061 ggatcgatca ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa
    15121 atatcttgcg gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt
    15181 ttaccaaagc gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga
    15241 gcaagaacat cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact
    15301 gttgtcatca tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct
    15361 tctaattctg cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt
    15421 ggattacctt tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt
    15481 gcagccattt tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact
    15541 tcatcttctt tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt
    15601 ttgtctgagc agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg
    15661 atcgcatgtt tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca
    15721 gtcaaggttt ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg
    15781 agtggttctc cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc
    15841 atactgattc gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta
    15901 ttttcgttca aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg
    15961 atcgctttcc cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca
    16021 cctaatgact catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct
    16081 ccagtcaacg ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca
    16141 gaaatagcat caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca
    16201 acagtggtca ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag
    16261 gattccagtg ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca
    16321 acgatagcga tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta
    16381 taaaaggaaa tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga
    16441 aagaattttt tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt
    16501 tttcgccgtt catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc
    16561 ttctttactt cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt
    16621 cgtacaaaaa aaggtactta ttttactata acatcctttt tttatttact tcaaataaag
    16681 ggcttatcga ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct
    16741 accaaaggat gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa
    16801 tttctcgata gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca
    16861 tgcgtgtaga gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta
    16921 tccgaaacaa gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga
    16981 ataggaacaa gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac
    17041 atgaagaaat tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt
    17101 atttgaatcc ttccgaggta caagaaagcc gagcttatcg agagctcggc tttttctttg
    17161 tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc ataaccttat
    17221 ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat cgcatgctgg
    17281 taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac taaagtcgat
    17341 tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata gccaggcgta
    17401 atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt cgttccaatc
    17461 gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc atttttacgt
    17521 acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact atgcatatgg
    17581 tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac atgtagtgtc
    17641 agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc
    17701 aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt ttgatagcga
    17761 tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc caatgattca
    17821 agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc gtgttccaat
    17881 tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt cccaactttg
    17941 gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt cgttagaaga
    18001 aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc aggcagaact
    18061 cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat ttcatggaaa
    18121 tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga atgatcgcgg
    18181 tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa atcttctgtt
    18241 gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat gtttttcgtt
    18301 ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat aaaaaaacta
    18361 ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc ttcattgaaa
    18421 atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg
    18481 gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc atttgtttta
    18541 tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga atgactgatc
    18601 caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat ccagtggaat
    18661 tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac atgacgatcc
    18721 gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa
    18781 cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat cgctacccac
    18841 atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc
    18901 aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact cgtgattcct
    18961 tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc
    19021 atgacatgga tgaagcgatt aaactagcaa gccgtatcgc catcatgagt gaaggccgtg
    19081 tgatcaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
    19141 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
    19201 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
    19261 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
    19321 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
    19381 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
    19441 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
    19501 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
    19561 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
    19621 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
    19681 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
    19741 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
    19801 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
    19861 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
    19921 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
    19981 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
    20041 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
    20101 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
    20161 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
    20221 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
    20281 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
    20341 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
    20401 agtctagttg ccggaatggt ttaacattat acagataaaa ataccgcaat catcaacaat
    20461 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
    20521 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
    20581 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
    20641 ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
    20701 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
    20761 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
    20821 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
    20881 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
    20941 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
    21001 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
    21061 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
    21121 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
    21181 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
    21241 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
    21301 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
    21361 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
    21421 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
    21481 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
    21541 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
    21601 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
    21661 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
    21721 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
    21781 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
    21841 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
    21901 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
    21961 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
    22021 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
    22081 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
    22141 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
    22201 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
    22261 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
    22321 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
    22381 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
    22441 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
    22501 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
    22561 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
    22621 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
    22681 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
    22741 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
    22801 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
    22861 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
    22921 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
    22981 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
    23041 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
    23101 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
    23161 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
    23221 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
    23281 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
    23341 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
    23401 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
    23461 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
    23521 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
    23581 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
    23641 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
    23701 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
    23761 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
    23821 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
    23881 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
    23941 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
    24001 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
    24061 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
    24121 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
    24181 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
    24241 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
    24301 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
    24361 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
    24421 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
    24481 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
    24541 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
    24601 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
    24661 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
    24721 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
    24781 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
    24841 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
    24901 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
    24961 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
    25021 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
    25081 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
    25141 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
    25201 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
    25261 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
    25321 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
    25381 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
    25441 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
    25501 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
    25561 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
    25621 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
    25681 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
    25741 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
    25801 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
    25861 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
    25921 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
    25981 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
    26041 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
    26101 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
    26161 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
    26221 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
    26281 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
    26341 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
    26401 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
    26461 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
    26521 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
    26581 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
    26641 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
    26701 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
    26761 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
    26821 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
    26881 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
    26941 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
    27001 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
    27061 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
    27121 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    27181 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    27241 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    27301 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    27361 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    27421 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    27481 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    27541 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    27601 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    27661 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    27721 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    27781 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    27841 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    27901 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    27961 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    28021 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    28081 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    28141 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    28201 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    28261 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    28321 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    28381 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    28441 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    28501 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    28561 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    28621 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    28681 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    28741 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    28801 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    28861 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    28921 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    28981 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    29041 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    29101 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    29161 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    29221 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    29281 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    29341 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    29401 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    29461 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    29521 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    29581 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    29641 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    29701 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    29761 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    29821 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    29881 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    29941 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    30001 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    30061 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    30121 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    30181 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    30241 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    30301 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    30361 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    30421 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    30481 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    30541 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    30601 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    30661 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    30721 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    30781 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    30841 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    30901 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    30961 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    31021 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    31081 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    31141 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    31201 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    31261 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    31321 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    31381 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    31441 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    31501 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    31561 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    31621 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    31681 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    31741 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    31801 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    31861 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    31921 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    31981 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    32041 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    32101 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    32161 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    32221 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    32281 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    32341 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    32401 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    32461 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    32521 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    32581 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    32641 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    32701 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    32761 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    32821 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    32881 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    32941 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    33001 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    33061 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    33121 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    33181 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    33241 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    33301 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    33361 acaaaa
//
LOCUS       DAZKCE010000018        32349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000018 DAZKCE010000000
VERSION     DAZKCE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.018"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1951
                     /locus_tag="ACG8KQ_000769"
     CDS             53..1951
                     /locus_tag="ACG8KQ_000769"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9103732.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2281..3000)
                     /locus_tag="ACG8KQ_000770"
     CDS             complement(2281..3000)
                     /locus_tag="ACG8KQ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9103733.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    3190..3237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3337..3669
                     /locus_tag="ACG8KQ_000771"
     CDS             3337..3669
                     /locus_tag="ACG8KQ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103734.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3656..5659
                     /locus_tag="ACG8KQ_000772"
     CDS             3656..5659
                     /locus_tag="ACG8KQ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9103735.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5678..6148
                     /locus_tag="ACG8KQ_000773"
     CDS             5678..6148
                     /locus_tag="ACG8KQ_000773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9103736.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6169..6753
                     /locus_tag="ACG8KQ_000774"
     CDS             6169..6753
                     /locus_tag="ACG8KQ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFP9103737.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6768..7754
                     /locus_tag="ACG8KQ_000775"
     CDS             6768..7754
                     /locus_tag="ACG8KQ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9103738.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7744..8055
                     /locus_tag="ACG8KQ_000776"
     CDS             7744..8055
                     /locus_tag="ACG8KQ_000776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9103739.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8077..9858
                     /locus_tag="ACG8KQ_000777"
     CDS             8077..9858
                     /locus_tag="ACG8KQ_000777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9103740.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9851..11227
                     /locus_tag="ACG8KQ_000778"
     CDS             9851..11227
                     /locus_tag="ACG8KQ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9103741.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11234..11869
                     /locus_tag="ACG8KQ_000779"
     CDS             11234..11869
                     /locus_tag="ACG8KQ_000779"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9103742.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11899..13254
                     /locus_tag="ACG8KQ_000780"
     CDS             11899..13254
                     /locus_tag="ACG8KQ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9103743.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13446..14840)
                     /locus_tag="ACG8KQ_000781"
     CDS             complement(13446..14840)
                     /locus_tag="ACG8KQ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9103744.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14932..15903
                     /locus_tag="ACG8KQ_000782"
     CDS             14932..15903
                     /locus_tag="ACG8KQ_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9103745.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15900..16682
                     /locus_tag="ACG8KQ_000783"
     CDS             15900..16682
                     /locus_tag="ACG8KQ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFP9103746.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16851..17222)
                     /locus_tag="ACG8KQ_000784"
     CDS             complement(16851..17222)
                     /locus_tag="ACG8KQ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9103747.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17356..17799
                     /locus_tag="ACG8KQ_000785"
     CDS             17356..17799
                     /locus_tag="ACG8KQ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9103748.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17842..18651)
                     /gene="yidA"
                     /locus_tag="ACG8KQ_000786"
     CDS             complement(17842..18651)
                     /gene="yidA"
                     /locus_tag="ACG8KQ_000786"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9103749.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18670..20037)
                     /locus_tag="ACG8KQ_000787"
     CDS             complement(18670..20037)
                     /locus_tag="ACG8KQ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9103750.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20021..20851)
                     /locus_tag="ACG8KQ_000788"
     CDS             complement(20021..20851)
                     /locus_tag="ACG8KQ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9103751.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21014..21451
                     /locus_tag="ACG8KQ_000789"
     CDS             21014..21451
                     /locus_tag="ACG8KQ_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9103752.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21527..22147
                     /gene="rpoE"
                     /locus_tag="ACG8KQ_000790"
     CDS             21527..22147
                     /gene="rpoE"
                     /locus_tag="ACG8KQ_000790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9103753.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22336..22842
                     /locus_tag="ACG8KQ_000791"
     CDS             22336..22842
                     /locus_tag="ACG8KQ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9103754.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22859..23758
                     /locus_tag="ACG8KQ_000792"
     CDS             22859..23758
                     /locus_tag="ACG8KQ_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9103755.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23845..24729
                     /locus_tag="ACG8KQ_000793"
     CDS             23845..24729
                     /locus_tag="ACG8KQ_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9103756.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24921..24992)
                     /locus_tag="ACG8KQ_000794"
     tRNA            complement(24921..24992)
                     /locus_tag="ACG8KQ_000794"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24957..24959),aa:Arg,seq:ccg)
     gene            25052..26386
                     /gene="rpoN"
                     /locus_tag="ACG8KQ_000795"
     CDS             25052..26386
                     /gene="rpoN"
                     /locus_tag="ACG8KQ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9103757.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26535..27626
                     /locus_tag="ACG8KQ_000796"
     CDS             26535..27626
                     /locus_tag="ACG8KQ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9103758.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27667..28668
                     /gene="gap"
                     /locus_tag="ACG8KQ_000797"
     CDS             27667..28668
                     /gene="gap"
                     /locus_tag="ACG8KQ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9103759.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28778..29968
                     /gene="pgk"
                     /locus_tag="ACG8KQ_000798"
     CDS             28778..29968
                     /gene="pgk"
                     /locus_tag="ACG8KQ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9103760.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30072..30827
                     /gene="tpiA"
                     /locus_tag="ACG8KQ_000799"
     CDS             30072..30827
                     /gene="tpiA"
                     /locus_tag="ACG8KQ_000799"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9103761.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30943..32241
                     /gene="eno"
                     /locus_tag="ACG8KQ_000800"
     CDS             30943..32241
                     /gene="eno"
                     /locus_tag="ACG8KQ_000800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9103762.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10672 a         5707 c         6890 g         9080 t
ORIGIN      
        1 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
       61 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
      121 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
      181 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
      241 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
      301 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
      361 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
      421 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
      481 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
      541 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
      601 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
      661 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
      721 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
      781 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
      841 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
      901 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
      961 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
     1021 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
     1081 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
     1141 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
     1201 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
     1261 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
     1321 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
     1381 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
     1441 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
     1501 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
     1561 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
     1621 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
     1681 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
     1741 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
     1801 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
     1861 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
     1921 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
     1981 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
     2041 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
     2101 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
     2161 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
     2221 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
     2281 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
     2341 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
     2401 ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
     2461 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
     2521 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
     2581 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
     2641 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
     2701 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
     2761 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
     2821 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
     2881 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
     2941 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
     3001 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
     3061 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
     3121 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
     3181 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
     3241 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
     3301 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
     3361 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
     3421 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
     3481 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
     3541 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
     3601 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
     3661 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
     3721 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
     3781 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
     3841 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
     3901 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
     3961 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
     4021 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
     4081 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
     4141 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
     4201 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
     4261 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
     4321 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
     4381 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
     4441 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
     4501 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
     4561 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
     4621 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
     4681 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
     4741 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
     4801 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
     4861 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
     4921 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
     4981 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
     5041 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
     5101 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
     5161 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
     5221 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
     5281 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
     5341 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
     5401 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
     5461 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
     5521 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
     5581 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
     5641 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
     5701 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
     5761 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
     5821 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc aaggtttgta
     5881 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
     5941 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
     6001 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
     6061 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
     6121 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
     6181 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
     6241 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
     6301 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
     6361 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
     6421 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
     6481 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
     6541 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
     6601 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
     6661 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
     6721 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
     6781 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
     6841 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
     6901 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
     6961 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
     7021 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
     7081 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
     7141 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
     7201 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
     7261 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
     7321 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
     7381 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
     7441 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
     7501 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
     7561 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
     7621 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
     7681 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
     7741 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
     7801 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
     7861 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
     7921 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
     7981 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
     8041 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
     8101 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
     8161 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
     8221 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
     8281 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
     8341 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
     8401 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
     8461 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
     8521 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
     8581 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
     8641 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
     8701 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
     8761 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
     8821 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
     8881 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
     8941 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
     9001 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
     9061 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
     9121 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
     9181 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
     9241 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
     9301 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
     9361 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
     9421 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
     9481 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
     9541 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
     9601 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
     9661 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
     9721 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
     9781 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
     9841 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
     9901 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
     9961 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    10021 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    10081 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    10141 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    10201 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    10261 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    10321 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    10381 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    10441 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    10501 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    10561 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    10621 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    10681 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    10741 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    10801 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    10861 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    10921 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    10981 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    11041 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    11101 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    11161 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    11221 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    11281 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    11341 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    11401 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    11461 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    11521 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    11581 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    11641 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    11701 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    11761 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    11821 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    11881 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    11941 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    12001 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    12061 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    12121 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    12181 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    12241 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    12301 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    12361 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    12421 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    12481 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    12541 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    12601 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    12661 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    12721 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    12781 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    12841 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    12901 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    12961 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    13021 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    13081 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    13141 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    13201 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    13261 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    13321 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    13381 tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca
    13441 ataggttata attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa
    13501 atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg cgcacgtgta
    13561 gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc
    13621 ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt
    13681 ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta
    13741 gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct
    13801 aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt
    13861 actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct
    13921 aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag
    13981 taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc
    14041 acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa
    14101 gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca
    14161 ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc
    14221 aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa
    14281 agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct
    14341 aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt
    14401 gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat
    14461 tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca
    14521 atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa
    14581 ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa
    14641 gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt
    14701 aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac
    14761 gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg
    14821 tcataagaat taataggcat tttctccact ccttaaaatt gtataccata caattttacc
    14881 attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt
    14941 attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa
    15001 aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt
    15061 ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac
    15121 agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct
    15181 tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata
    15241 aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac
    15301 ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg
    15361 catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta
    15421 gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt
    15481 ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc
    15541 tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct
    15601 gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa
    15661 atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg
    15721 gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag
    15781 aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct
    15841 attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa
    15901 tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag
    15961 cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg
    16021 aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat
    16081 ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg
    16141 ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta
    16201 atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga
    16261 aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct
    16321 acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc
    16381 cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa
    16441 gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc
    16501 cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa
    16561 caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag
    16621 aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt
    16681 gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat
    16741 ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa
    16801 ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg
    16861 atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca
    16921 ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc gtgtaatttt
    16981 tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca
    17041 tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca
    17101 agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt
    17161 tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc
    17221 atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt
    17281 tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta
    17341 aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat
    17401 caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag
    17461 gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa
    17521 gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat
    17581 ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca
    17641 ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat
    17701 aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga
    17761 tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct
    17821 ttctctcagt ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg
    17881 ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc
    17941 cattgctact cctagaccag catattcaat catggataag tcgttctcgt tatctccgat
    18001 agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc
    18061 tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta
    18121 tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc
    18181 gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt
    18241 tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg
    18301 cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccattac
    18361 ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt
    18421 cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt
    18481 caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga
    18541 aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt
    18601 ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc
    18661 caatatattt taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg
    18721 ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg
    18781 aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac
    18841 ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct
    18901 gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta
    18961 ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga
    19021 atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc
    19081 taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac
    19141 cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag
    19201 taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg
    19261 aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac
    19321 atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa
    19381 agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc
    19441 atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat
    19501 ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc
    19561 taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg
    19621 agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt
    19681 atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg
    19741 ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat
    19801 atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc
    19861 tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac
    19921 ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac
    19981 tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat
    20041 gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca
    20101 gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct
    20161 tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca
    20221 gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt
    20281 cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt tcctgattac
    20341 cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac
    20401 cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga
    20461 tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct
    20521 cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat
    20581 tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt
    20641 aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca
    20701 tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt
    20761 tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa
    20821 tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat
    20881 tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa
    20941 aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa
    21001 aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa
    21061 agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac
    21121 aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat
    21181 tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat
    21241 gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata
    21301 cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc
    21361 cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga
    21421 atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt
    21481 ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt
    21541 atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga
    21601 acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg
    21661 gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga
    21721 cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga
    21781 ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg
    21841 ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa
    21901 tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga
    21961 cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc
    22021 agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat
    22081 catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa
    22141 agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa
    22201 ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc ccaagtaaat
    22261 acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc
    22321 agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac
    22381 cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg
    22441 aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc
    22501 ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata
    22561 ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt
    22621 gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg
    22681 attttagata aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga
    22741 tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa
    22801 atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat
    22861 gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc
    22921 caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa
    22981 agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat
    23041 tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat
    23101 cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat
    23161 cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg
    23221 attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata
    23281 tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga
    23341 taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga
    23401 aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt
    23461 caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca
    23521 agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat
    23581 agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt
    23641 agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt
    23701 cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac
    23761 gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag
    23821 agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct
    23881 ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa
    23941 ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc
    24001 attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa
    24061 aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg
    24121 gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag
    24181 cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat
    24241 acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat
    24301 cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg
    24361 acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg
    24421 ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg
    24481 tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa
    24541 gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt
    24601 gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg
    24661 gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca
    24721 gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt agttgaacac
    24781 ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc
    24841 accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta ttcgtaaatt
    24901 atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga
    24961 atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt aactaaacag
    25021 ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa
    25081 acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca tccaagtttt
    25141 acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg aaaatccatt
    25201 ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt catcttctta
    25261 tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt ctttatttga
    25321 ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga aaatcgttct
    25381 ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag aagaagccgg
    25441 aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga ttcagcaatt
    25501 agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg
    25561 agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg aggaactggt
    25621 ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg ctgttcagca
    25681 gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg accgtagcag
    25741 cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa atgtacaagt
    25801 tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact tcaaacaaat
    25861 gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac aggaattcga
    25921 atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat
    25981 tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc ctcttacgtt
    26041 aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg
    26101 gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca ctacacgaat
    26161 tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga agaaaaagct
    26221 gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga aattggttga
    26281 attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat atcgtgattt
    26341 attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag tcaaaagact
    26401 agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca aaactgttag
    26461 acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat agttaatttc
    26521 tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt ccaactaagg
    26581 agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat attatcgaag
    26641 tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg attggacgtc
    26701 gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa acagatcttt
    26761 tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca actaagggcg
    26821 aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg catcaaaccg
    26881 aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca ggaaacagcg
    26941 aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac
    27001 tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg gctgtggtag
    27061 ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc cctgcaagag
    27121 gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga atggctgcta
    27181 aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg gcaacttaca
    27241 attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt gaagcgaatt
    27301 gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag atgactgaaa
    27361 aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc tactttttca
    27421 atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg caagatttgg
    27481 aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag
    27541 cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg
    27601 agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat aaggaggaaa
    27661 tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg tctagcattc
    27721 cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt gacagatgct
    27781 aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa cggaacagtt
    27841 gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc
    27901 aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga atgtactggt
    27961 ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa acgcgttgtt
    28021 atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa ccacgaaaca
    28081 ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg tttagctcct
    28141 atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac aactatccac
    28201 gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga cttccgccgt
    28261 gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa agctatcggt
    28321 ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt tcctgtacca
    28381 acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc
    28441 aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt
    28501 tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca aactaaagta
    28561 atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa cgaaatgtca
    28621 tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga tagacaaacg
    28681 tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc gcttcttccg
    28741 ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa cagtgaaaga
    28801 tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga
    28861 cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca actacgttct
    28921 agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga
    28981 taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat taggtaaaga
    29041 agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa
    29101 tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga
    29161 aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg tatttgttaa
    29221 cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg cttcaactgg
    29281 tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt
    29341 agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt ctgataaaat
    29401 cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg gcggaatgac
    29461 ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag aagctgataa
    29521 agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct tgccaatcga
    29581 caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg aaggagacat
    29641 tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat ttgctgatac
    29701 attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa
    29761 ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag gcgcaacaac
    29821 aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg ctgacaaatt
    29881 cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta aagaattacc
    29941 aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct gacgagtaat
    30001 cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata aattaaggag
    30061 tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac aaaactgcat
    30121 cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg
    30181 attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca gctgaaggta
    30241 cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg ttcactggtg
    30301 aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc ggacattcag
    30361 aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca aaagctatct
    30421 ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc tatgaagcag
    30481 gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat ctttcagctg
    30541 aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt actggtaaat
    30601 cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac
    30661 tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct gtaaaaccag
    30721 aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa
    30781 gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg ttgaaaattt
    30841 gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc gtatatgagg
    30901 gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat tattactgat
    30961 gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt agaagtatac
    31021 acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac tggtgaatac
    31081 gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa
    31141 gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga tgttcgtgac
    31201 caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa aggtaaatta
    31261 ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc tgactaccta
    31321 gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc aacaccaatg
    31381 atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca agaattcatg
    31441 atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc tgaagtattc
    31501 cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt
    31561 ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc
    31621 gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc tgcttcttca
    31681 gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga aggcgaaaaa
    31741 acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc aatcatctca
    31801 atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac tgacgtatta
    31861 ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca aaaattatct
    31921 gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca aatcggtaca
    31981 ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac agcagttgta
    32041 tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt tgcaacaaat
    32101 gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa atacaaccaa
    32161 ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt gaaatctttc
    32221 tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac tattgaaaga
    32281 tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa atatcagcag
    32341 gtagagagt
//
LOCUS       DAZKCE010000019        32069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000019 DAZKCE010000000
VERSION     DAZKCE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.019"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1082)
                     /locus_tag="ACG8KQ_000801"
     CDS             complement(183..1082)
                     /locus_tag="ACG8KQ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9103763.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1092..1448)
                     /locus_tag="ACG8KQ_000802"
     CDS             complement(1092..1448)
                     /locus_tag="ACG8KQ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9103764.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1803..2099
                     /locus_tag="ACG8KQ_000803"
     CDS             1803..2099
                     /locus_tag="ACG8KQ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9103765.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2622..3575)
                     /locus_tag="ACG8KQ_000804"
     CDS             complement(2622..3575)
                     /locus_tag="ACG8KQ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9103766.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3598..4353)
                     /locus_tag="ACG8KQ_000805"
     CDS             complement(3598..4353)
                     /locus_tag="ACG8KQ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103767.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4350..5309)
                     /locus_tag="ACG8KQ_000806"
     CDS             complement(4350..5309)
                     /locus_tag="ACG8KQ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9103768.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5302..6252)
                     /locus_tag="ACG8KQ_000807"
     CDS             complement(5302..6252)
                     /locus_tag="ACG8KQ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9103769.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6402..6854
                     /locus_tag="ACG8KQ_000808"
     CDS             6402..6854
                     /locus_tag="ACG8KQ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9103770.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6943..9210)
                     /gene="gshAB"
                     /locus_tag="ACG8KQ_000809"
     CDS             complement(6943..9210)
                     /gene="gshAB"
                     /locus_tag="ACG8KQ_000809"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9103771.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9397..10395)
                     /gene="ftsY"
                     /locus_tag="ACG8KQ_000810"
     CDS             complement(9397..10395)
                     /gene="ftsY"
                     /locus_tag="ACG8KQ_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9103772.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10406..11215)
                     /locus_tag="ACG8KQ_000811"
     CDS             complement(10406..11215)
                     /locus_tag="ACG8KQ_000811"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9103773.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11224..14805)
                     /gene="smc"
                     /locus_tag="ACG8KQ_000812"
     CDS             complement(11224..14805)
                     /gene="smc"
                     /locus_tag="ACG8KQ_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9103774.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14825..15511)
                     /gene="rnc"
                     /locus_tag="ACG8KQ_000813"
     CDS             complement(14825..15511)
                     /gene="rnc"
                     /locus_tag="ACG8KQ_000813"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9103775.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15859..17640)
                     /locus_tag="ACG8KQ_000814"
     CDS             complement(15859..17640)
                     /locus_tag="ACG8KQ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9103776.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17666..18580)
                     /locus_tag="ACG8KQ_000815"
     CDS             complement(17666..18580)
                     /locus_tag="ACG8KQ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103777.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18607..19569)
                     /gene="opp4B"
                     /locus_tag="ACG8KQ_000816"
     CDS             complement(18607..19569)
                     /gene="opp4B"
                     /locus_tag="ACG8KQ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9103778.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19569..20513)
                     /locus_tag="ACG8KQ_000817"
     CDS             complement(19569..20513)
                     /locus_tag="ACG8KQ_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103779.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20514..21518)
                     /locus_tag="ACG8KQ_000818"
     CDS             complement(20514..21518)
                     /locus_tag="ACG8KQ_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103780.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21786..22028)
                     /gene="acpP"
                     /locus_tag="ACG8KQ_000819"
     CDS             complement(21786..22028)
                     /gene="acpP"
                     /locus_tag="ACG8KQ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9103781.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22150..23151)
                     /gene="plsX"
                     /locus_tag="ACG8KQ_000820"
     CDS             complement(22150..23151)
                     /gene="plsX"
                     /locus_tag="ACG8KQ_000820"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9103782.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23200..25236)
                     /gene="recG"
                     /locus_tag="ACG8KQ_000821"
     CDS             complement(23200..25236)
                     /gene="recG"
                     /locus_tag="ACG8KQ_000821"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9103783.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25353..27035)
                     /locus_tag="ACG8KQ_000822"
     CDS             complement(25353..27035)
                     /locus_tag="ACG8KQ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9103784.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27087..27449)
                     /locus_tag="ACG8KQ_000823"
     CDS             complement(27087..27449)
                     /locus_tag="ACG8KQ_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9103785.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27719..27907
                     /gene="rpmB"
                     /locus_tag="ACG8KQ_000824"
     CDS             27719..27907
                     /gene="rpmB"
                     /locus_tag="ACG8KQ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9103786.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28145..30814
                     /locus_tag="ACG8KQ_000825"
     CDS             28145..30814
                     /locus_tag="ACG8KQ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9103787.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(31269..32015)
                     /gene="truA"
                     /locus_tag="ACG8KQ_000826"
     CDS             complement(31269..32015)
                     /gene="truA"
                     /locus_tag="ACG8KQ_000826"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9103788.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8897 a         6870 c         5691 g        10611 t
ORIGIN      
        1 gttcaaaaaa agaatacacg cttgcatcta gtactctgcg agttacaaat catccgatca
       61 aataaacatt cgctgattat tttccgcaaa aaaagcagca tatccacgat aaatggatct
      121 actgctctta cttatcctta tgttttacaa ggtattttct tttacacatt actaatgctg
      181 tctcaatacg gaacgattgt catcgcttgt tcaaattcct cttgcaaatg ttgtgtttga
      241 taggcctgaa aagctgggtg atgggcacgc aagaggacac gtaatttcat aggatcatag
      301 ctgtctagca actgttcttt caagacaaaa tctcgggtca gctgccattc ataataataa
      361 ccaaaaggtc tgcagctgta aatcgtgtga ccgatggatt tatcttcaaa gcgacgatgc
      421 gtatggccaa aaactacttg tttgatattt gaataacggt caagtaattc tccgaatgat
      481 tctgatccta aaaatgcatt taatttgttc caacgctcat aaggagcaga ttgatacatg
      541 ataaatgccc gtttaggaac gaaatgagtg gataaaatca cctcaagttt ttttgcttcc
      601 agctggtcta gcaaattttt tgcagctgca agcatttctg cattgacctc gagatctgtt
      661 ttcccacgct ggatgaaccg atcataccaa tacaaacgct tcattcgtac gattgcctcg
      721 taatctttca attcggaaaa ctgatagtcg taccagccat tcatccctaa aagaacggtc
      781 tgctcattta aagcaatata ccgttcattt aaaaaatgtg ggtctggaaa atggtccatc
      841 atctgttcat tgggcacatc tgccaactcg tgattgccga aatgaaatgt cgtagataat
      901 cctttattga cgaaaaattc gacaacagct aggcaacggt ctactttatt agccgtatct
      961 ccggctagat gaagccgagt gatcttgtgc ttttgcaaaa cttgccaaag ctgcatcagc
     1021 tctgattcgc cgaatttatt gatgtccaca tgtaggtcgc tgatgatcgc caattttccc
     1081 attttatccc tctagtcttt tcgaacaact tgcaagttgc cttttctcaa gccgcttggt
     1141 ccttttaatt gtaaactatt ataagttggt tctaaagcaa gtgtggatgg atcatccatg
     1201 taaagtattg cactgtcttt gattttgatt ggtccaactg gtgtatcagc cactacagaa
     1261 taaagactta gatctgtttg tgtatctttt ttgacaatga tcaatcgaaa tgccgtatcc
     1321 aattggcaac tgacttggct ttctgcaaat ggaccgtcac cgttttcgat atctaataaa
     1381 agttgatcgt ctgcttctaa tttattcttc aatacttctt gtgcttcttt tgtcacttct
     1441 aatttcatct gtatcacctc atgtcataga ataatcactt tattcatcag atgcaactaa
     1501 tttgtctggt tctgactgtt ctcaatttat tggaaagtaa gtcattcgtt tattatgatt
     1561 tctcttatca caaaagagac aactttccct tttcttcgtt cagtttcttg aacggaaaaa
     1621 gacccaaaaa gcaacctgtt atttgtttaa ccttattcct ttcttaaata acaaaaaata
     1681 ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt tgttcacgga
     1741 ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca
     1801 ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt
     1861 cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt
     1921 ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa
     1981 tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg
     2041 atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa
     2101 tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt
     2161 tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata
     2221 gcttctcata ttttctagtc tagtggactt attgccgaga agcagcagat ggaacatcgt
     2281 ttaaagaaga gatcggtaca aaataaaaat atctaaattt aagttttatc taagtttcgc
     2341 tttgtacctt tgattcaaat caaaggaggc gatcttgatg ctgaaaaaat ggatcacact
     2401 attttttgcc actccccatt ctagaatcaa cccgtatgat gtagataatg aattcacgtt
     2461 gaaaaaagat ttctcaaata aataaaaacc atccgtaaaa tcatcaatac gattttacgg
     2521 atggtttttg ataacttctt tgaggatagg ctcttgctat ttaagaatgt aagaaacatt
     2581 attgttacat agtatgagag tttaatcctc tgttatttat gctatttaaa tgcttgattc
     2641 acatcttcga tcatcagttt cattgattct aaaccgcccc cgcttaaata ccagacatct
     2701 ggttcaaggg agatgatctg ctgattttta ccagcattcg tttgtgctac cagttcattg
     2761 gcagaaatat catctttgct gtcgtctcca ccaatagctt ttgttctgtc tactacaaat
     2821 aagatatccg gatttttttc cagcacatat tcataagaaa cactttgacc gtgagtggat
     2881 gcttcgattt gatcatctgc ttgttcaaag ccaaataaat catggatgaa accgaatcta
     2941 gaaccagatc cgtaggctga taattgccct tcattcacaa gtgtgactag tgcttttttg
     3001 tcagtagctt ctgctttctt tttcgtctga tcgattgcac tagataattc ctctaattga
     3061 gttttagctt cttcttgctt accaaagatc tcccctaaag cagtgacatt ttgctggaaa
     3121 gattcccatg gatttttcgc atctaaagaa aggaaaatgg tcggagcaat ttgacttaga
     3181 tctttttgaa aatctctttg tcggccagaa atgatgatta gatctggctg aagctgatta
     3241 attttttcta agtctggttc ttttattcca cctgctgatt ctactttttt ataggatgaa
     3301 agatactcag gaaggctgct tgtagctgct ccaactacct tatctccaac accaagagca
     3361 tccatcgtat ctaaagaacc attgtcgaat acgacgactt tttctggatt atttggcacc
     3421 tcgatttggt ctccattact atctgttatc gtcaatgtag tttcagtttg tgtgctgctt
     3481 ttttttgtgt ctgcctgact ggtgttgttg ctatttccac atgctgctaa accaaatgat
     3541 gctagaatca agatacctac tactaatttt ttcatctctt ctctcctaat gttagtttta
     3601 gaaataaaga cagaatctgc ggccttcaat ctcacaaatc cgtatgtcca tttcgtataa
     3661 ttcatccaat acatcttttt ttatcatttc ttgggtcgtt ccttgcgtaa ataactgtcc
     3721 atctttcatt gcaacaattt cgtctgcata acttgcagca aagttgatat catgaagaac
     3781 aataacaatt gttttcccga tttcttgtac taaatttttt aaaatctgca tgatttgaac
     3841 cgcaaaattc atatctagat tgttcaaagg ttcatccagt aaaatataat ccgtgtcttg
     3901 cgctagcacc attgcaatat acgcacgttg gagttgaccg cctgaaagag tgtcaatcga
     3961 ttgatccgct atttcttcta acccaatgtg ttccagtgat tcctgaacga tttcctcgtc
     4021 ttttgtcgtc aattttcctt tcgtataagg aaatcgtcca aattctacta attctttcac
     4081 ggtgatttgg acatttattt cattcgattg tcgtaggatc gataattttt tggctaattc
     4141 attggatttc caagccttta cttcgttatg gtctaaatag atttcacctg tatcttttgg
     4201 tatcaaacga ctgatcatcg acaacaccgt tgactttcca gcaccatttg gaccgataaa
     4261 agcagtcaac ttcttttcag gaatcgttaa ctgtacatct ttaaccactg attttccatg
     4321 gtacagttta ttgacctttt gtaaatcaat cataggatac tcttcctttc gcgaatcaat
     4381 tttgtgacaa aataaattcc tccgacaaat tcaacgacga cactcactgt tgtattccag
     4441 ccgaaaatct gctctactgc gagctgtcct cctgtcagaa acagcatagc taccagaaag
     4501 cttccgataa atagctgacc atgacgataa gtagaaaaga cacgataact gatatttgcg
     4561 acaataaacc ctaaaaaagt aactggaccc acaagagccg ttgatgtccc tgtcaaaaga
     4621 ctgacacaac ataaaatcaa aaattcaaaa cgatctgtgt ctatcccgag gtttactgct
     4681 tgttcatttc cgagatggaa aacgtctagc acatgtcgat atcgaaataa caggtaccag
     4741 cagccgataa tgatcaaaga agcaggcaaa agatactgac tctggatatt gccaaagctt
     4801 gcaaataatc tgccttgaag caaatcatat tcattcggat ccatgatgac ttgtaaaaag
     4861 gtactgacac taccaaaaag cgtacctaaa atcatccctg acatcagtaa taaaaaaaga
     4921 ttcgctctca tttttttcaa aagaaagaaa aataacaata gactcagccc gaccatcagc
     4981 ccaacgttca ataagaaaaa aactgtacct gtctgctgaa gcatcgtcac tcctccgata
     5041 aagaagaaaa ggaccgtttg gacaaggaca tataacgact caagtcccaa tactgaagga
     5101 gtcaaaaact ggttatgcgt aattgtctga aagctgattg tggcactgct gacagctagc
     5161 gagacaagga taaatgcaaa aattttctta ctcctcaatg cacatgcaaa atgccagttt
     5221 ccatatgtat ggatcgtcaa gtacattcct gtaacaaata aaacaagaac aagcatacca
     5281 ataaaaatag atggcttttt tttaagcata tcgtcgtccc cttatcagaa gataaaggaa
     5341 gatagcccca ccgatgattc caacgatcac actgactggt atttcataag gaaaaatcaa
     5401 cacgcgagcg acaagatcgc aaaatactaa gaacaaacta cctgctaaag cagttacgcc
     5461 aattgttttg tgcacttgat ctccatatat tctagagaca agattcggaa tgatcactcc
     5521 gataaaagga atgcttccta ccatgatcaa catgactgca ctagccaatg atactaagcc
     5581 aatgcctaaa aactgcatct tctggtaatc tattcccaga tttactgcaa acgattcgcc
     5641 cagaccaatg atcgtaaatc ggtaagcaaa tagccaaacc aagagcaaca atggaacaac
     5701 agcatacaat aactcataat ttcctcgtat cacagtcgca aaattccctt gaagccatga
     5761 agtcatattt tgtaccaact ggaattgata agcaaaaaaa gtagcaactg aaccaatgat
     5821 attccctaac atgactccaa tcaacggaag catcaattga tttttctgga ggaacaatcg
     5881 cgtaagggat aaaaaaagca atgttcctgc aaaagcaaag caaaatgctg tcaaggatcg
     5941 gacaatcaag gatgcaccag gtaggaaaat catagccact aaaataccta aacgcgcact
     6001 atccatcgtg cccgcagtcg ttggtgaaac aaatttattt tgggtcaaat gttgcatgat
     6061 caacccacaa atactactgg tcgctcccac aaggatcaaa caaatcgttc gtggcaaacg
     6121 agtcactagc aagatttgta agctttgccc atctaactgg agcagttctg acaattgtac
     6181 attcgatact ccgatgaaca atgaaatact acttaaaata actaataatg ctactagtcg
     6241 tccgtagcgc atccaatcca cctttctaat tgagaatgat tttcattctt attttaagtg
     6301 aatcgttgaa caaaagcaac cgctttttca tatgttttag gtattattgg gaatatgcta
     6361 taatcgtaag aaagacaaag agaagaagga gggtattttt tatggcacaa gatgatgtaa
     6421 aaagccattt ggataaggga ctttatggta cacctttagt gaaccctgaa gaacagcata
     6481 aatatttagg gacattcagg gaacgctgct atcttagtat gactgttgct gaaatgatcc
     6541 aaccaaaaaa caaagaaaac tttttaaaag aactcaaaaa acatccagat gcttccatac
     6601 tcttgaatgg ggctctaccc ctttctgtcc aaaaccaata tatccagcta gcaacgaaaa
     6661 acaatagccg tttcactgta gtcaacgatt acgtcactgc aaatcctgag gaattcggat
     6721 tgctcttaac agcaaaagaa gcagtaaatg aacctgtgat tgatatcgaa aaaaaatacc
     6781 cagaagaaaa ctcttcgcca gctgaaaaag aaaaaccaaa aaagaaaaaa agcttctggg
     6841 atcgtttatt ttaaaacgaa aaaacacata aatttttaca tgcaaaaaaa gcttttgatc
     6901 agattgacat caaataggac aatctgtcaa aagctttttc ttttaaaata cttcaggata
     6961 taacaatttc aatacattca tcgtcaatct tctgcctttt cctgcaaaag gataaacatg
     7021 catatgcatg gctggattga aatttgcttc gatgatccca tatgcttttt tatcagttgt
     7081 aggatcgatt tctttatctg gtataatcaa atcgatacca ctaatcttag ctcctagtgc
     7141 ttctaccgca gaaacggcga tttttttgta agcttcagaa aattcatcag tcatatcgat
     7201 cgaatcacca cctgtactga tatttgagtt ctcccttaga taaacaattt gattggcttg
     7261 gggaactgat tcgatcgtca acccttgttc tttcaacatc aattgttcta attcacctaa
     7321 ttggatcaat tctaaaggtg cacgatgatt cgttccacgc aaagggtcac tatttttttc
     7381 ttcaacaagt tctttcactg ttcggatact gtcaccaata acattagcag ggactcgcaa
     7441 catgatggct tggacttctc cgtcgatcac aaagaaacga tattccgttc ccggcataaa
     7501 ttcttctact aaaaccgaac tgtcttcacg aaaagcaatg gctaaacctg cttgataatc
     7561 ttccagacta gctccttcct tgaaaatcgt gatacctaac ccgtaattgg ttgatttagg
     7621 tttgatcaca aatgcctgct cggcaaatct cggataagct tttaccgctt cttccatgga
     7681 tgaaaattct gcgcctcctg gtacccggaa cccagcttct tttaagactt ttttcgttac
     7741 tgttttgttt tccatgataa gtgggacgat atagctgtcc ttacttgtca tgtttgcatt
     7801 tttcacatat tctacatggt cttggaattg cagacgcaaa aattgatccg actcatctaa
     7861 tactttgact tggacacctt tttggatggc atcgaacata aatatctgag tagaaagttc
     7921 catttcgcga aaaccggcca actgataggg acgttcatgg ctttctttat aatattgatg
     7981 tcccaatgcc acagcaactt cttgctgact cgatagctgt gcttcttgat agagtttcgc
     8041 tgctaacgtt tgttcaggat gatccatttg agtcaatgct tcctctacta aataaagaga
     8101 atccaaccaa tccaattcaa ccagcatttg cttcattcct tcaagaattt ctctaccttc
     8161 ttgatgaaat tctgtttgat ctgatggttg ttctagtgct actttgttat ttcgagtcgt
     8221 accttctgct acccattggt cacagtcttc tttttcttct gtccataaca tgtacatcag
     8281 gaataattgt aagaaccgaa cttgctctgg attgataccc aatcttgcat aaggattcaa
     8341 atcgatattc cgcaattcaa tataacgaac acctgttgtt gctaaatctg cgactttctt
     8401 tccgcctcgg aaacgaagcg gtgtataaaa ttctttctcc tccgaaagtt tcccttcttc
     8461 gatcattcgc tcgatatctg ctaaatactg tttcatagaa gcataggaaa ctttcacatt
     8521 tggatgattc gtatagccca gcgcactgtt gcgaatactg cgaacaggtt cttgcggatg
     8581 tgattcatct aaaaagtagt tcttctcact cattggtgaa gcaccgaata aataagtgat
     8641 catccagcga tagcgcataa agtttctcgc tgttttcaaa taaagttctg ttttgaattc
     8701 ggaaaaatct tgaaattctt cctgatggct gaacaatgtt cttagcaact catcgccaaa
     8761 ttcaaaattg aaatgaatac cgcttaccat ctgttttcgc ttcccgtatt cttttgctaa
     8821 ataacgtcga tacaagacat cttcgaaatt ttttaatttt gcaataatga tctcttcgtc
     8881 tttttccggt aaggcaggtg gcatacttaa tggccagatc atctcttctt tcggtataga
     8941 acgagcagtc acatcataaa cagcagcgag atactgaaat aattcgggaa tagaatcggt
     9001 aacaggggtg atcatctctg tttgtgtttc gctaaaatct gtttggatat aggggtgata
     9061 ggatcgatcg ccaaagattg ctggatgatc ggtttttgct agatttcctg caagatcaac
     9121 ccgctgtcct tcccgttcta taccaaatcg agcttggtcg ataaaagggc gcgcattgac
     9181 atgcaataat aattgcttaa aattcatcat taagtgacct ccaattcata tgaatctaat
     9241 tataatcaaa aagtctaaga aaaagaacga aattgtttga gaaccgctta caaataagta
     9301 taaaaatgaa ggcatcaaaa acacagctat gttaaaaaca atcattcaaa caaggagctg
     9361 caacttttgt cacagctcct gctttttaga catgggttat tcttctttta gtaatccttt
     9421 gaacaatcca acgacaaaat catttggatc aaacggctcc aagtcatcga ttccttcacc
     9481 aagaccgacc aatttcactg gtaaatgcaa ttcattacgg atagcaagta cgataccgcc
     9541 ctttgctgta ccatctaatt ttgtcaaaac aagaccggtc acatctgttg tttctttgaa
     9601 ttgttttgct tgaaccatcg cgttttgacc agtggttgca tctaatacaa gaagtgtttc
     9661 ttggggtgct tctggtaatt cccgctggat tactcgtttt attttttcta attcgttcat
     9721 caagttcact ttattttgca gtcggcctgc agtatcaact agtaaaacat ctgcctgctc
     9781 tgtctttgct ctttccaaag catcgaatac gacagctgct ggatcgcctc ctgcattgcc
     9841 acgaacaact tcaacaccag cgcgttcgcc ccagacaact aactggtcga ttgctccagc
     9901 gcggaaggta tctgctgctg ccatcaatac ttttttacct tcgtttttga actgatgggc
     9961 aagtttacca atacttgttg ttttccctac accattaaca ccgacaaaca aaataactgt
    10021 tagtccgtct tcttggatat ttagttcatt tacttcattg attccttccg cttcatagag
    10081 atccacaagt ttctcgatga tcgcattttg gacttctgcc gattttttcg catttctgag
    10141 tttcacttct tgacgtaatg cttcggtgat tcgtatcgct gtttcaaaac ctacatctgc
    10201 accgataagt gtttcttcta attcttcaaa aaagtcttca tcgactgttc gaaaattggc
    10261 aaacaactca ttcatccgtt caccaaatgt tttacgcgtc ttttctaaac ccttgtcgta
    10321 cttctcctgt acttcaactt cttctgtttt ctcttcgcca gtaaaagcac gcttgatttt
    10381 atcaaaaaat cccatttgac tccccctact ctaatacaaa tttttggatc actttggcta
    10441 cgccattttc ttcattgctg gctgtcacga aatctgctgc ttctttgacg aaaggcacag
    10501 cattctccat cgctacgcct agtccagcat attggatcat cggtaaatca ttttcctcat
    10561 caccgatcgc catgatttct tcttgtttca aaccaagatc ttttgccaat aaagaaatac
    10621 cataagcttt tgtaacacct tttggcataa attccaaaag catacttctt gttttgatga
    10681 tctcatacat ttcatggaat tctgctggta tttctttgat tttaggatct aattcttctt
    10741 ggtcataagc gattactgcc ttattaagca caaattcatc tgtaatatcg cttatatccg
    10801 ctggttggaa ttgcagcagc ttattcaaca cattgtacaa ggattgcttc cttggggcac
    10861 taggcagctg taaaaccaca ttgtcagaca aaacatctag tggcagatcc aattgctgag
    10921 ccagtttgta taagcgttgg atatctgata cttccatcac tttcttttcc atgatttcac
    10981 cagtatcatt tttttgtacc aaaccgccgt tgaaagtgat gctgtagtca ccttcatcta
    11041 ccagtcctaa ttcttgcaga taatgcgcca tcgcagctaa ggggcgtcct gtacatagaa
    11101 cgatcttgac cccttgctgt tttgcttgtg ctaatgcttg tttattctca tctgaaatca
    11161 gcttttgttc atttaataat gtaccgtcca aatcgattgc tactaatttg atcattttcg
    11221 ttcctacact ttcgtttcgt tgattttcgc ctcttcattg atatcttcca tccgtacaga
    11281 aacgattttt gatacaccgg attcctgcat cgtcactccg tacagtacat ctgctgcgac
    11341 catcgttcct ttgcggtgtg tgactacgat aaattgtgtg tcatcttgga aatcgctcaa
    11401 gtaacgacca aaacgtttca cgttggcttc atctaacgct gcttctacct catctaagat
    11461 acaaaacgga acaggacaga cacggataat ggaaaagagc aacgcgatgg ctgtaagtgc
    11521 tcgttctcct ccagaaagca aacttagact ttgcagtttt ttgccaggag gttgtacttc
    11581 aatctcaata cctgttttca gcaaatcagt tggttccgtc agtactaatt cagctttacc
    11641 gccgccaaac atatttggaa agacaatttt gaattcttga cggatagctt caaatacttc
    11701 tttgaatctt gtccgcactt catcatccat ctcatccatc gtttcaaaca attggttttt
    11761 cgctgctaac agatcatctc gctgtgttgc taagaatgta tgtctttcac tgacttgttc
    11821 gtattgttcg attgaattta agtttactgg tcccagtttt tcaatctgtt cttttaagga
    11881 tgaaaccttt gttctactgt cttcgatatc ggttgtttct tgatagtctt gggaagcttt
    11941 ctcaaatgtc agctgatatt ctgactgcag ataggtcaaa tgattatcta aaatcatttc
    12001 agctcggtct ttttggactt ctaatttgga ttgttccgac agtcgtgctt tttgctcacg
    12061 attacgctct gccaagactg cttcaagctt atcgatctcc ttttgccttc tatctcgctg
    12121 ctctttggct tttgccaact cggcttttaa tgtttctcgc tgagcagata attcgttaat
    12181 ttgtttttcc aaactttctt ctgtgacttc atgatcagaa aagtctgctg tcaatgtcgc
    12241 caactgcttt tctatcgctt cttgacgttc ggttgcctct gctttttgga ctcgggcacc
    12301 gcggagctgg atctgcagat gattaaactg ctctttcaag acagcaagat ctgcttgttc
    12361 ttgtgctttt tgactttgta cttgtgctct tctggcttcc atctggtcgc tttcttgact
    12421 taacgatttg atttcttcgt cgattttttg tcgttgcgat tcaagatctg tttgtttgtc
    12481 tttcaattcg ttcgtttgct tttcgtaatc ttcgataaac tgctgcagtt cacgtgtctc
    12541 aaaagttgag atttgctttt ctttttcaaa acgttcaagt tcatttatga tgttttgtag
    12601 ctgatttgtc gcttcttgtt cttcaaaacg taattgttcg cctcgtgtac gcaaaacttc
    12661 ttgttcttcg gctaaacgag ctgtttcttg ttgcagctcc tgaacttttt tttcttgtgc
    12721 ttggagttgt ttatctgctt cttcaaattc actagtcaac tgtttcaatt cttgattttg
    12781 tacaaacaaa ctgccttggt ttcctcgttt attcgctcca cctgtcatgg atcctccggc
    12841 attcatcaca tcaccttcta aggaaaccac tcgatattga tagcggatcg tttgcgcgat
    12901 tgcattggca ctagttaagt ctttggctaa aaggatcgtt cctagcaggt tttgaaccac
    12961 agtttggatt tgatcgggat aactgacttg ttcactggca atccctataa acccttctac
    13021 agctgctgct tgtgtcaata tatgagccgg taattgtctt ggtttgattg tcgtcagtgg
    13081 aaggaatgtt gctcttcctc ctctttgctg tttcaaatac gtgatggctt gccgagcatc
    13141 tttttcattt tcaacgatga catgctgggc tgccccacct aaagctgctt cgatagctaa
    13201 cgtgaaatct gcaggaacat cgatcagttc agccactgct ccgacaatac ccgaaagctg
    13261 ctgtttatgc tgcaagacca atcgaacccc ttggtaaaaa ccaaaatagt tttcttggat
    13321 ttcttgaagg cttttcttac gagcacgtag ctgctggact tgattcatta attgatacat
    13381 tttaggctgt tcttttgcta atgcttcttg gaactttttc ccgttttgtt ggatcatttc
    13441 taacttctct tttgtttcag ttaaagatga ttttagctgc gcctgctttt gtgtcaattc
    13501 ttctttttgt aagatcaatc ggtctactga tgcttctact tccgattgct tggctaacgt
    13561 ctgcttgctt tttgctgttt cttgtatata ttgacgttct agatacttca gttcgtttcc
    13621 tacagctgct tgttcttgca tcaaatcaac atattgatct cttaattctg ccaataattc
    13681 tttcgttgat ttttggtatt tttctaattc ttgctgtgtt tttatgagat ttgcttcagc
    13741 tttttggatc tctgtttctt tttctgctgc tgctttcatc aagctttctt gaagcttttc
    13801 aaaatgttta accttttttt gtgcttctgc taatgaagtt tgatattctt gcgagctttt
    13861 ttgtgtatgc tttgtccgct cctgcaaaac atctttttgt ccttctgtct gcttcagttt
    13921 ttccgaaaga tctaataata cttgctgatt tttttcgatc agacgatcgg cttgcgcatt
    13981 ttctttacgc tgcttcgcca aaatggattc ttgttcttgg atcgactcag atagtttgcc
    14041 taattctaga ttgaatttcg caagctgtgc ttgtttgttg tcccaatctt tttttgctgt
    14101 cttgatttca gcaaccatca aagagacatc ggtttgagtc agtgtttctt ttagccgtaa
    14161 aaactccttt gctgcctcac tctgggcggc caaaggagtt aactgttctt ccaattcgtg
    14221 gatgatgtct tggacgcggc tgaggttgtc ttctgtttca aaaagcttct gttctgcttt
    14281 ctttttccgc tgcttgtatt tcaagacacc agctgcttct tcaaagatcc cacgacggtc
    14341 ttccggttta ctactgaaaa tcgcttctac ttttccttga gaaatgattg agaacgattc
    14401 ttttcctaat ccagaatcta agaacaattc ttggatatct tttaatctgc agctttgttt
    14461 attgatgaaa aactcgcttt ccccagtccg tcgataacgg cgggttacac tgatctcatt
    14521 gtactccaat ggcagataat gatcactatt gtctaaaatc actgtgactt ctgcaatatt
    14581 cagctgctta cgggtatcgg aaccggcaaa gatgatatcc ggcatttttc cgcctcgcag
    14641 acttttagca gattgttctc ctaataccca gcggatagcc tctgtaatat tgctttttcc
    14701 gctgccattt ggtccaacca ctgctgtgac gctattttca aaatcaatga ctgtcttgtc
    14761 tgcaaatgat ttaaaaccgg ctatttcaat tcgtttcaaa tacaaagctt tccctcactt
    14821 acttttacag tgctgccaat gcattttccg ccgcagcttg ttctgctaat tttttcgatt
    14881 tcccttgtcc agtgccaatc aattgatcat ctacataaac ttcgatccaa aatactcgtt
    14941 catgagctgg accttcttca ttgatcaatc ggtattcgat tgatacatca cctgattttt
    15001 gcaagacttc ttgtagtttt gttttatgat ccatctcatg tgaaaaagca cctgctttga
    15061 ctttaggaaa aatcactttt tcgatgaatg catgggtcgt gtcgaatccc tggtcaagat
    15121 ataacgcgcc taaaaaagct tcgaataggt cgcaaagcaa tgctggtcgc gtacgtcctc
    15181 ctgaattctc ttctcccttg cccaacaaaa catattgatc aaagtgacat tctttggcaa
    15241 atttcgacaa gctgtcttcg cgaacgatcg cagctcgtgt ttttgtcaat tttccttctg
    15301 gcaaatgagg atataaacgg aataaatact gagaaaccat cagttctaaa acggcatctc
    15361 ctaaaaattc aagtcgctca ttatccgata attgcagatt gcgatgctca ttcacatagg
    15421 atgaatgggt aaaagcttgt tcaagcaaat ttagatcgtg aaaaacgatg ttatagtttt
    15481 cttttagttc ttttgtcagc tggttgtcca ttttccacat tccttttatg gatatttttt
    15541 cgatacttcc ttattatact ggttttttgt caaatttgaa atggttcggt caaaaggtaa
    15601 gtaaagcttt aataatccta gtgattgacg agaagttgtg aaaaattcgt ttagtttcga
    15661 gtatgaagaa aaaattttgt gaaaatggct ttccatattt ttgcataaat ttgacttaat
    15721 accgagaaac tggactttga acaccgttta ttggagaagt catgaaatca gcgccttgac
    15781 ctgaatatta gagaaataaa cagaaaaata tcaaaaaacc gaaaaaggac atcgcccttt
    15841 ttcggtcatt tttagtgatt atgaacgtga atctgctgta acagaaacat ttgcccatgg
    15901 gtttggtaca tcataagacc aatcccagtc tttgacacgg tcgttgactg gcaatacttc
    15961 attacggaac aacgttggaa tcgcaaatgc ttcttcaaat gcatattgtt gccatttttg
    16021 atatgcttct ttttggtact cttcatcaaa tgctttgctt gaactgatat cgttgatcaa
    16081 ttttgtattt tcttctgatt cataacgagt gtagttgaac gcagcatttg gtccccatag
    16141 tccacttgga tttggatcag ttcctgtatt ccaagcagct tggtaaacat caatttcagg
    16201 atcatcgttt tctaatttat catagaaaga attgaattcg atcagacgac ctgttgtgta
    16261 ttggacatcc aaaccaattt ctttccattg ttggatatag taatctgcca atggttgcgc
    16321 tgtttcccca ccggacatcg aagcaaagtt gattgttagt ttatttccat ctttgtcttc
    16381 tcttaaacca tcgtcatcca catctttgta gccagcatca tctaatagtt ttttcgcttt
    16441 gtctagatct tgtgtgaatc ctttcatctc atcatcatgg tatgactgga atactggagg
    16501 aatcaatgaa gtcgcattgc ttctcaaacc tgcatagaaa cgttcaccta ctgcattatt
    16561 atctaatgca tagcccattg cttgacgtaa tgatttatca gccatttttg aatctggatt
    16621 gtattccaca ctctttgtat ctgcattcca tttacctaac ttgaatccta gataagtata
    16681 agaagtttgt tcacggccta agtttgtata gccagcaatt tctttatagg tatcgtaagt
    16741 atctgttggc atttggaaaa tcatatcata taatttagag ttcaatgctt cagttgcact
    16801 tgccatcggt acagatttca tcgtgatctt tttcaattta ggcgtaccat tccagtagtg
    16861 ctcgtttggc aagtattcta ctgattctcc tgcaacgata ttactcatgt aataagggcc
    16921 aaaagtcact ggatttttac gtacttgatc gcttgattcc atatctttga ttgctacacc
    16981 ttctaacgta tgttttggtg ctgcatatga ccagatacca ccacccgatt gaagcatgct
    17041 tgggtttact tctttatatt cgatttcaac tgttttgtcg tctatttttt tgattccgga
    17101 gattgtgtcg gcacttccat catgatattc ttccatacct acgatgttac ggaaagtgtc
    17161 gtcataacgg ataccagtgt aatctttatt cccaatgatt tcgtaaggga aaatcacatc
    17221 gtccgccgtt acatcttcac catctgacca tttgacgttg tcttttactt tgattgtcgc
    17281 ttttttcgta tcttcatcta gatcaagcgt tgcagctccg tcgtctgtga ttctgaaatc
    17341 gtcatcgttt gcaaatagcg gttcatgaga aggagccata aaagctgcat catatgaatc
    17401 ttggtaaaac tcccactgga acaatccttt aaactgactg tccgttgcga ccgcagcttc
    17461 taattcgccg tcttcgattg catcatcatt attttttgtt gcaattggaa aagttaattc
    17521 ctctttgtta ttgccgttgc ctccagagtt gtttccagag gtatctgtac ctccgccccc
    17581 gcatgctgca agaacgacag cagacatcag cgctgcaaat ccaaaaatcg ttttcttcat
    17641 tcctatttcc cccttcgctt ctatactagc ctaaccgttg gcgcgcatct gcagaacgct
    17701 tgaatgcttg tccaacataa ttgatactca acatcaaaac taaaataagg attgatgcgg
    17761 gtaaccagat ccattgccga ttcactaaga cttctccgtt acttgcataa ccgatcaatg
    17821 tacctaaact aggtacattt gccggcaagc cgaaacctaa gaaagtcaaa gttgtctcga
    17881 taccgatgtt tgccgcaaag ttcatcgtca aattagtaat gattaatgaa cttagattcg
    17941 gcatgatttc gcggaacatg attttaaaat cattggttcc cattgttttt gaggcactga
    18001 cataatctct tcttgcttca gataatgtct tgcttcggaa taatcgggct ttagctaccc
    18061 aataaaatgc actcatgatg aatataaagg accagacatt gtatctggaa ataatcgata
    18121 cgaagacgat aatgatcagc atgatcggta gaatcatgat aaaatcaacg atccgcatca
    18181 agatgttgtc aatgatccct ccgtagtagc cagagatgat ccctaatcca accccgatga
    18241 tcgaagtaag gatcgtgatc gcaaatccga tcgtaacaga gtttctagca cctatgatca
    18301 attgtcctaa tacatcacgt ccgccttcat ctgctcccag aatgaacttt tgtccttgtg
    18361 tcgaaactgt tcctggtgca gcgtatttgt cgaagatact aacatacatc actttgtctt
    18421 gatcggtcag catcgcaccg ataaagatca ctaataataa aaccactagc aaacctaatg
    18481 aaaaaatagc aattttatct tttttaaatt ctcgagcaat cattcgtatc cccataggag
    18541 gaatactttc ttgaactgct gtattgtttt tttcactcat cgatccattc tcctttctga
    18601 ttggttttat tgaatccgaa tacgcgggtc aacgatactc atgatgatat ctgaaagtaa
    18661 tgtccccact agcgtcgcga ttcctaaaat caatactaag gatgtgatca cggtatagtc
    18721 ccgttggccg atggaatcaa taaatagttt tccgactccc ggataaccaa aaatcttttc
    18781 gataactaca gaaccagcaa tcaacgaagt gatttcatac cccatttgag aagccatcgg
    18841 caacgctgca tttcggaaaa tatgtcgact gaatacttta ttcgtcggta cccctttgga
    18901 acgggctgtt ctgacaaaat cgagcgattt cgaatcgatt acttcacttc gcagatattg
    18961 gattgtgacg gcagtcgcca aaaatgcttg ggtaattgat ggtaacagaa tatatttcaa
    19021 acggtctatc cattgagtga taaatccacc ttgcaaacct agtgtttgtg atccacttgt
    19081 tgggaaccag cctaaacgat aaccaaaaac aaatagcatg atcaagccga agataaataa
    19141 aggaacagca aaactaaaga agttgtagat tacgacaatc ttatccaaga ttgaattttg
    19201 ataccggcca gcaagcatcc ccagaggcac agcaatcaaa taagtaagga caactgtcaa
    19261 gatcgataga tataaagtgt tgatgatccg ttcgccgatc aagtcagcaa cagagcgttt
    19321 gtataagaaa ctttgaccaa aatcgccttg caaagcattt ccgatccaac ggaagtactg
    19381 gacataccat gggtcattca agccagctgc ttgtcgcatc tgttcgatca ctgccggatc
    19441 ctgatttgga ttgatcaaac cggtaaatgg atcccccggc atcgcttggg ctaataagaa
    19501 gatcagaaca cttaaaataa taacttgcgg gatcatcaat aaaacacgtc gcagaatcgt
    19561 tttccacatt agcttgcacc tccatctttc aacgccactt tgtgtgtagt tgtgatcgta
    19621 cgcaaatcgt agactcgacc agtcgcatca tagtattctt tttggttttg gatatactct
    19681 tgttctactc ttcgtcgatt ttctttgtgg ctttccctat tttctacatc gatttgcgga
    19741 atagctgaaa gcagacgttt cgtatagata tgtcgcgggt cattataaat gtcttctctt
    19801 gtaccgattt ctacaaatcg acctttgtac ataattgcga tattgtcgca catgtgtttt
    19861 actacaccta aatcatgtga aatgaataaa tagctgaggc cgtattcttg ctggattcgt
    19921 ttcatgaaat tcaatacttg cgcttgaaca gatagatcca gagcagatac aggttcatcg
    19981 gctacgatca gttttggatt ggtagcaaca gcacgggcta ctcctagacg ttgacgttgt
    20041 ccgcctgaaa attcatgagg atatttatat agcgcatctt ccggcatccc aacgatatct
    20101 agcagtcctt ttaccttttt cttttcttct tgatcgctta accgttcaaa atttcggatg
    20161 ggttcagcaa tgatatccaa cactcgcttt ttaggattca aactggacat tgaatcttgg
    20221 aagatcatct gcacatcctt gttataaccc atttttttgc ggttgcttcg cttcgttaca
    20281 tcttttcctt catacatgat ttgtccggct gttacctttt ctaatcccac aacggctttt
    20341 cccgtagtag attttccaga gccagactct cccactaaac catatgtctt tcctttttcg
    20401 ataataaaat ccacaccatc tacagcatat acatgatccg ttactcgatt gaagaatcca
    20461 ctgcgaatag ggtaatgaac tttcaggtct ttgattgtga ttaattctgt catctacacc
    20521 tctccttttt cgtctctaaa gtggaaatgc ttatagcagg agcaacgtac caagtggttc
    20581 ggtgcgactt catgaaggat aggctcttct tcatgatcgc ttgctggtat ccatggaata
    20641 cgtggtgcaa accgacaacc tttacgaggc atgtttttca gagaaggaac gacaccttcg
    20701 atcacatgga gttctgcatc atgatttcct tcttgtggta tagagttcag taaagagcgt
    20761 gtataaggat gcttaggatg atcaaacagt tcttttacac ctgctacttc aacgaactgt
    20821 ccgccataca tcactgccac tcgatcagcc atttctgcta cgactcctaa gtcatgagtg
    20881 atcaagatga tccctgtttt tgtttcttcc tgaaggtctt tcagtaaatc aagaatctgt
    20941 gcttggatgg ttacatctag ggctgtcgtc ggttcatctg caatgacgat tggaggtttg
    21001 caggcgattg cgattgcgat gatgacacgt tgtctcatac cgcctgatag ttcatgggga
    21061 tattggcgtc caactctcgc aggattagga atccctactt gttctagcaa ttctagtgca
    21121 cgagcttgac gctgttctgc attcatcttt gtgtgatagg aaagtccttc tttgatctgg
    21181 tcttcgattc tcatgagagg atttaatgca gacaacggat cttggaaaat cattccgata
    21241 tcatttccgc ggattttatt atagagcgtt tcgttcaggt cgattaaatt cagatcgtta
    21301 tataaaatct cacctgtaat ttttgtgtta ttaggatcaa gtaatcccat aatcgtagta
    21361 gccaaggtac ttttcccgca cccagattcc ccaacgattg ctaaaatctc gtttttgtct
    21421 aaagtaaatg aaacgtcatc tactgcatcg taatagtcat ctttgagtcg gaatcctgta
    21481 tgtaggttct gaacttctaa taattggtta tcggacaatc gaatacccct ctctgcttcc
    21541 taaatggaag tttttcttaa taaaaaagga cattacacag aataaatcgt tttataacaa
    21601 aggttattct tatctttgtg taatgaatta ttaataatta tatagaaaac cgctttactt
    21661 tgcaacataa tttctgttta tcgccagaat tatcagaaaa atgactttta cgtgtcaaaa
    21721 agattaaaaa aagaaggata aaaagctgca tgcagctttt tatccttctt tgcttttatt
    21781 tgaaattatt taagatggct gctgatataa tctactgcgc caccgactgt ttcaatttgt
    21841 tcagcgtctt catctgaaat ttccgtaccg aattcatctt caagctctaa aacgaattcc
    21901 ataatactaa ttgaatcagc ttttaaatca tctttgatgt tcaattcatc cgtcacttta
    21961 tcggtatcta tctcaaaatg atttgaaata attttcgcta ctttgttaaa tacttcttcg
    22021 cgagtcaact ttttcacctc caagaatcat acacttgtca attacctaca ccatggctaa
    22081 ttttactggc atttttcagg aatgtccacc tttttttcaa tggaacggat cgcttcagat
    22141 atccaaatac tattcttcgc ttttttcaaa ttgttcgact aattgtccaa ctacatctgt
    22201 ctcaagcatt gtgtggattt ggcggatcgt atagcgaaca gcatcggggc ctgttgctcc
    22261 atgagtttta atgactggtg ctttcaagcc aaacaaaacg gcaccgccat gtttggaata
    22321 atccatttct gctttcaaag aacgtaagcc gtcttttaaa agcatagcgc ccatttttcc
    22381 tttgatccct tcatttagaa tagcagattt taatagattc atcatattca ttgctgttcc
    22441 ttcaatagat ttcaaaacag cgttgcctgt aaatccatct gtgaccacta catcagccac
    22501 accattgagt agctcacgag cttctacgtt tccaataaaa ttcaagcttg tttcattctg
    22561 aaggagttca taggcttttt tcgtcacttc actacctttt gttgcttctg ttccgttgtt
    22621 taagagtgcg acacgaggac gtccgatacc tcggacttta cgtgcataaa aagaacctaa
    22681 aataccgtat tgcaacaaat gttctggttt gttttctgcg ttagcgccaa gatccagcat
    22741 atcgaaaccg gcaccttctt tccctactac tggcaaggta gacatcaagc cagggcgttc
    22801 gatatttttg atacgtccta cgatgaacaa ccctgctgct agtagcgcgc ctgtattccc
    22861 tgctgaaaat acagcatctg cttcaccgtt tttcactgct tgtgcagcaa ggaccattga
    22921 tgcagtcttt tttcgtcgaa ttgcttttac tggctcatca tcactattga ttttttcgtc
    22981 tgtatggata atcgtgatgt ttttttcatt tgttaagtat ttcttgattt ctgcttcttt
    23041 tccgtaaagt aagaattcta cttctggaaa atcttgctgc gctaaagcca cgccttctac
    23101 aattgcttga ggtgcgtgat ctccgcccat tgcatctacg gcaattttca ttgtttccac
    23161 tcctttttcc gtcatcagta taaagtatag cacagtctgc taatcgaaat aacgattatc
    23221 ctgattttct atttggaggc tttgcgctaa aggctgatac tcaggaagca tctgccaatc
    23281 ctttatttgc caaagggctg cagcctcttc tctggctacc tccaaaacat ttccatccgt
    23341 caccaagtca gcaatcgcaa attgaggcag accagattga cggaagccaa atacttcacc
    23401 aggaccacgt aattccagat ctttttcgct tagtacaaaa ccattggtgg tctctgtcat
    23461 gattttcatc cgttccacac ctagttcatt tttaggatta gcaactaaga tacagtagga
    23521 agcatcactt ccgcgtccaa cacgtccacg aagttggtgc agctgtgcca atccaaatcg
    23581 atccgcatcc atgatcagca tgacggtcgc gttcggcacg tttaccccta cttcaattac
    23641 agtagtcgaa actaatatct gcatctggtt gtctttgaag gcttccatga ttacttcttt
    23701 ttcctgattc ttcattttac catgtaacaa gccaacttca tattttggtg caaatagttc
    23761 acgaagtttt tcatagattt ccactgcatt ttttacatct aatgcttctg attcttcaat
    23821 cagcggacag atgacataca tttgatgtcc tcttgccagt tcttttttcg tccaatccag
    23881 tacactgtcc aattgaggtg ttcttaccca gcgtgtttcg atcgggatac gtccagctgg
    23941 aagttcatca atgatagata catccatctc accatatgct gtaatagcta atgttctagg
    24001 aatcggagtc gctgtcataa ataagacatc tggtcgccag cctttttcac gaagtacttt
    24061 ccgctggttc acgccaaaac ggtgctgctc atccgtaata actagaccta aattctgaaa
    24121 ttcgactcca tcttggatca atgcatgtgt gccgatcacg acgttaattt ccccttgctc
    24181 tagtttttca aggagctcac gtcgttcttt tgctttcgtt gaccccgtaa tcaatgcgat
    24241 ggttacttca ttgggatcaa acagctgttg caagctttcc atatgttgtt gtgccaagat
    24301 ttctgtaggc accatcaacg caccttggaa acctgctgta acggtagcgt ataacgcaat
    24361 cgcggcaacc actgtttttc cgctacctac atctccctgc aaaagtcgct gcatatgctg
    24421 cggactgcga agatcccgac agatttcatt cgttacacgt ttttgtgcat ttgtcagttc
    24481 aaaaggtagc ttttgagtaa attgtttcaa tttttggaca tcataaagga tctccaaacc
    24541 attcgtttct gctttttctg cttttttcaa tccttgaaga cgcatttgaa ataaaaagaa
    24601 ctcttcaaaa acgacacgtc gtttggcttg atggttttct tcgtggtctt ttggaaaatg
    24661 catacttctt acagcagaag ctcggtcaag cagtcgatat ttctccacaa ggttgtttgg
    24721 cagattttct tcaatcaatg tgccgtattc ttgaaaccct tttttgatta gatcaactaa
    24781 tgtcgtttga cgaattgatt tgttgacgtg atatatcggc gaaaagtcat ccattgtttg
    24841 agaacctaaa atcttcatcc cgttcaaggc tttacgcttt gcatcccatt taccgtatac
    24901 ggcgatttcc tccgataaaa tgactttttc ttttagataa ggttgattaa aaaaagaaac
    24961 gttgaacaca tcatgttctt gcatcatacg aaattgcaat cggctttttt tataaccaaa
    25021 tcgattcatc acaggaggcg agactacgat tcctttcaaa gtaacctttt cttgatcctg
    25081 gatctcgttt agatttcttt cctgaatatc ttcgtagcga aaaggataat aagtcagtaa
    25141 gtcttcaatc gtttggatgc ccaagctggc aagactagct gctctttttt ctccgactcc
    25201 tgccagcata gtgattggat catctaaaga acgcataaat ttttcctccc tcctttaaca
    25261 aaaaaaggac ttggtcagcg atcatacctg atcactaacc gaaggccttt tatgaatgaa
    25321 tactgtgtta gaaagatatt ctgacacaga aattattcag cagaaaatag ataaggatag
    25381 acaggctgat ctccttggtg gatctcgatt tccaaatcag gatgtttttc tgttagtttc
    25441 tcagcaaatt catttgcttg ttcaactgtt ccttcatcac ctgccaagat cgttacgatc
    25501 tctgtgtcct cattgatcat ttgttctagt gtacttaacg ctgttgctgt gatatctggg
    25561 ttagagatca ggatttttcc atcgatcatg cccaaaaagt ctccttcttt gatctctaca
    25621 ccgtcaattg aggtatctct gatcgcgtga gtgacttgac cgctcacaac gctagacaac
    25681 atttctgtca tcgtttcttt gttttcttct aaagaagctt gatcattgaa tgaaagcata
    25741 gctgtcaatc cttgggaaat cgttcttgaa ggaacaactg ctactgggat atctgctact
    25801 tctgctgctt gatcagctgc catgaagatg ttcttattgt ttggcaagac aatcacttta
    25861 tctgcgttta cttcttgaat cgctttaaca atgtcttccg tacttggatt cattgtttgt
    25921 ccaccactga taacataatt agcgcctaaa ctttcaaaca gctcttggac gcctttacca
    25981 gcagcgatcg ctacgatacc atacgggata cgtggtattt cttttttcgc aaactcgttc
    26041 acttcttcat cgtgttccaa aatcgtctca tgctgcaagc gcatgttgtc gactttgatt
    26101 ttgactaatg aaccgaattt ttgtccataa ttcaagactt ctcctgggtt ttctgtatgc
    26161 acgtggactt tgatgatctc gtcatcattg accacaagta aagaatctcc tataccatct
    26221 aaatagttac ggaattcttc gtaatcgaat gtactatcga ctgtaggtcc ttctccgatt
    26281 tttaccatga tttctgtaca gtaaccaaaa tggatatctt ctgtcgccat ttgaccttgg
    26341 acactgcgat gatgttctgc attgaccata tcatccattt ctgctggaga aggttcatat
    26401 gtttcatccg cttggaattc tccattcaat gcacttaaaa agccctcata aacaaacagc
    26461 agtccttgcc ctccactgtc tacgacaccg acttctttta ggactggaag caagtctgga
    26521 gttttagcta aagcacgctt tgctcctttt acaacagctg tcattacttc aacgacatca
    26581 tcagtacttt ttgctttctt ttctccagct ttcgctgctt ctctagctac tgtcaaaatc
    26641 gttccttcta ctggtttcat tactgcttta tacgcagttt caactccatg agtgaaggct
    26701 gcagcaagat cagaagcatt taatgagaca acatctggaa tttgcttaga aaatccacga
    26761 aacaattgag acaaaataac gccggaattt ccgcgcgcac ccatcagcaa tccttttgaa
    26821 agcattgctg ctaattctcc tactttttct gaggttgaat ctactactgc cttagctcca
    26881 ctagtcatag atagattcat atttgttccc gtatcgccat ctggcacagg gaatacattt
    26941 aatgaattga cgtattctgc atttacttgc agacgattcg cacctgcctg gaccatttcc
    27001 tggaactggc ttgcacttat ttctgttaca ctcacctaga taatcctcct tcagatcgac
    27061 tgctgagtta ccagccagtc tcctgcctag tctggcagta cacgaacacc ttgtacaaag
    27121 acattaactg aattggcagt tacgcctaat aatgcctcta gattgtattt cactttttct
    27181 tggacgttgc gggaaacttc agagattttc gtaccatagc tgataatcgt ataaacatct
    27241 acagcaatac cattatcttc ttggcggaca actactcctc ttgagtaatt ttctttacgc
    27301 aaaatctcag taatattatc tttaatttga tttttgctgg ccattcctac aacaccgaac
    27361 acatctgtcg cggcgccacc aacaactgtt gcaattacat cattggtaat ttcaatggtt
    27421 ccagctgacg tttgaatttt tacagccatg aatgagcctc cttaacgggt aaaaaactcc
    27481 catttttctt ctatcatatt ttatcatagc gcaacttgaa ataaaaggca gttacccaaa
    27541 gtactcaaaa accttgcaaa atttcaggta taaccttgtt taatttacaa ttattcttct
    27601 caattgtcaa ttgtatctat gaaaatgtac ttgcaaattc ccattctata tgatatgtta
    27661 gtctggtatg agctgaatta ttctattgct ccaaatcaag gcaaaggagg aaattaccat
    27721 ggcaaaagtt tgttacttta caggtcgcaa gacaaaaagt ggaaacaacc gttcacacgc
    27781 tatgaactca acaaaacgta ctgttaaacc taacttacaa aaagttcgcg ttatggtcga
    27841 cggaaaacct aaaaaagttt gggtgtcaac tcgtgctttg aaatcaggaa aagttgaacg
    27901 cgtttaattg aatgatactt ggaccgttca gatagtgaac ggttttttat ttaataaaaa
    27961 tttttaaggg tgggactttt gattcctacc ctttttttgc gtttattttt gttatacgaa
    28021 caaattatga agttatttat tctaaatttt atttttttgt gaaaactaga ctctttttaa
    28081 gcagagaaaa acaaggatat gataaactga atacaacttc atagaaattt ggtggaaaat
    28141 aaatatgaaa aaaaatttaa agtcattctt tagagaaaac ggacgctacg cagcagctag
    28201 tttcctgatt ccgtttttga ttttagcact tgtttatcta agtatcggaa tctatcctgg
    28261 aagcagcaga agtattttgg caagtgatgc cttttcacag ttttctaatt ttcatgccag
    28321 cttccgaaat gttcttcttg gaaagcaaag tattttttat acatggaatg cttctctagg
    28381 attgaattat ttatctttaa tttcttacta tctaggcggg atcttcacgc cactggtgtt
    28441 cttttttcca aaccagatga tgccagatgc actgtatgtt ctgactttgc ttaaggtcgg
    28501 ctgtgccgga ttggcttttt ggtttcttgc acgtacgttc aagattcctc gctgggggca
    28561 tgttgcactt agcgtcagtt acgcattgat ctcatttatc actgcacatt cggagatcat
    28621 catgtggctg gatgcatttt tttatcttcc attagtgatt ttggggattc atcgtgttat
    28681 ggatcttaaa cggccgaaac ttttatttat cagttattta ctcttgtttt tatccagttt
    28741 ttatatgggt tttatgattg gcgtattttc tttcttatat tttatcgccc gtctattgac
    28801 aaactggtca gaatataaaa aaacgattgt tccttatggt atcacttctt tactggcagg
    28861 cggcgcttca atgatcattg tattgccagc agtgctggat cttcggtcaa atggcgaggc
    28921 actgagtcag atcactacgt tgaaaacaga agcgacttct tatctggatc taatcatgaa
    28981 aaatatgatt ggtgtttatg acacgacaaa atacggatcg attccgttcg tttacgcagg
    29041 tctgctacca ttagtcctct gtctgtttta ttttgccagt cggaaagtac cgttgaaaaa
    29101 taaatttttg tacggcagtc ttttcgccat acttatcgca agtttttatc ttgttccatt
    29161 gaatttgttc tggcatggta tgcatgcacc gaatatgttt ttattccgtt atgcctatct
    29221 gttctctttc ttagtgctcc tgcttgcagg aaaaggatgg gaaaagatag aacaagacga
    29281 tcgtgggctg ttgttaggaa tcattttggt cgtagctgct ctctttgcgg tggcatgggg
    29341 catcaaacct gaaggaagct acacctatgt gacgatgact tcctttgtat tgacagtggt
    29401 tttcttaggg atttatgctt ttgctgtggg attttatcag cataaaaaac tttcattcaa
    29461 tcatctgagt atattgttat tattgatcat gtctgctgaa gcactggtca atacgaacag
    29521 tatgatccat ggcattcttg atgattggaa ttatgcttcc cgcagtttat atactgatcc
    29581 ttatcctgac ttgaaagctc ttgtaaatga aacaaaaaaa gattccgact cgttttaccg
    29641 cttggaaaat ctggatccag tatctgcaaa cgattcgatc aactatggtt acagtggtat
    29701 cagtttgttt tcttctataa gaaatcgtaa ttcttcctct tacctggata aattaggttt
    29761 ccgttcaaga gggacaaatt tgaatatccg ttataacaat aatactttgc tgatggatgg
    29821 gtttacagga atcaaatata acatttctaa aaacgaaagc acgttcagta aatatggctt
    29881 ccgccaagct ggtcaaagcg gcgactatac actgtatcag aatgtcaatg cattgccact
    29941 agcatttact gcaccgttgt ccgttaatga tgttgagcag ccagtgacag ataatttgac
    30001 cagtcagaca aacttgatca atacattgtc tggtctaaat gaacagtatt ttacattcta
    30061 tacacctact ttgaagaatc agttgaatac gacgatcaca aagacaacga ctggcgtcac
    30121 ttactcagaa acagcagata accagccaaa agagctcact tgggaagtcc atgtgcctgc
    30181 aaatacacaa gcttacttaa gtttgttccc aaccaatttt gcgcaattag aaagttctag
    30241 tgcgacgatc actgtcaatg gtacatcgcg aaaaacgcaa atcaacattt ctggtcaata
    30301 ctatgatctt ggctattatc ctcaagagac aacagtgaca tttactgcaa gtttttatgg
    30361 gacgaaagaa ataagtttca tggagcctaa agtcgttggt ctagatgttg tagcctatca
    30421 agccgcaatg aatcagatca aagaaaaagg tgtcgaaatg gaagccactg gcagaaaagc
    30481 ttctgggact gtaaataccg ctgaagattc catgttggtg acaactattc cttatgacaa
    30541 aggatggaaa gctaaaattg atgggaaacc agtcaaggtc agcaatttca aagacgcatt
    30601 cttgatgata aaagttccag caggtaaaca tactgtcact ttcagttact tgcctgaagg
    30661 atttacgata ggtgcaatcc tttttgtcct atgtattgca ttgtttattc tttatgtctg
    30721 gtaccttgac cgcaatcgcc ccttttatgg cacgattgat ccagcaatag aagaacaacc
    30781 acgaaacaca cacagaagaa gacgtagaaa ataaagaaac gatggaacct gttcagactg
    30841 tccgcaccaa gggcaatcaa aacaacgttc catcgttttt tggttacaga aaaaataggt
    30901 aacaaaaaga tgattcactt atcaacgcct cgtccacaag tcacgatatt catcaaagat
    30961 gagaagctgt tttttgaaat tgctctttgt gactcagagt taaatgacag gctcacgacc
    31021 tctttcttaa acggtgttca atcagctgac cttcggtatt agctcaacaa acggcaaaat
    31081 atgaggagct attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacga
    31141 cctcttcctt aaacggtgtt ccagaagtag cttcttcgaa aataagccga aattttgcaa
    31201 aattttgaga agtaattttc gtaaatacct tcttatttct tgaagctgac cacttctatc
    31261 ataacctctt attcatattt cacttcatac aaataaagtc cttcagggtg tgctgtaggt
    31321 cctgctgcat tacgatcttt tttcgcaata atatctggga tactatcttc aggcaatcgg
    31381 ccattgccaa ttttcaaaag cgttcctact aagatccgta tcatcttata caagaagcca
    31441 tttcccctaa atgtaaacaa caattcgtct tttgtttcat ttacctccat cttagcctcg
    31501 tagatcgtac gtattttatc ctctatcgag cttcctgatg cacaaaaaga ggtgaaatcg
    31561 tgagtcccta ataaatcagg aagcgcccgc tgaatttttt ccaaatcaag tgggtaagga
    31621 aaataacttg cataatggcg tcgaaaagga ctgcgtggtt tccctatatc cactcgaaat
    31681 tgatacgtct tttctttgac caaatatctt gcgtggaagt cttctgaaac aatttctact
    31741 tgtcttacag caatatcctc tggtgactgg gtatctagcg cgaaacgcat tcgttctaac
    31801 ggacgctcct ctggataatc aaaatggatt acttgcccga tcgcatgtac tccggcatct
    31861 gtccttcccg aaccaaagac cgtgatttct tttccatttg ccattttttg taatgttttt
    31921 tctacttctt cttgaacagt acgcccattt ggctgttttt ggaatccatt aaaatttgtt
    31981 ccgtcgtacg caatgattgc tttgtaacgt accatgtttt tctctccttt gttcctaaaa
    32041 ggttatctgg aataatgatt tttctataa
//
LOCUS       DAZKCE010000020        31975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000020 DAZKCE010000000
VERSION     DAZKCE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.020"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8KQ_000827"
     CDS             <1..186
                     /locus_tag="ACG8KQ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103789.1"
                     /translation="VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAA
                     IITQLFLVFGEKKRSKK"
     gene            complement(390..755)
                     /locus_tag="ACG8KQ_000828"
     CDS             complement(390..755)
                     /locus_tag="ACG8KQ_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103790.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            963..1445
                     /locus_tag="ACG8KQ_000829"
     CDS             963..1445
                     /locus_tag="ACG8KQ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103791.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1642..2532
                     /locus_tag="ACG8KQ_000830"
     CDS             1642..2532
                     /locus_tag="ACG8KQ_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP9103792.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2857..5328
                     /locus_tag="ACG8KQ_000831"
     CDS             2857..5328
                     /locus_tag="ACG8KQ_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9103793.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5431..7167)
                     /locus_tag="ACG8KQ_000832"
     CDS             complement(5431..7167)
                     /locus_tag="ACG8KQ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9103794.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7164..7868)
                     /locus_tag="ACG8KQ_000833"
     CDS             complement(7164..7868)
                     /locus_tag="ACG8KQ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9103795.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7999..8502
                     /locus_tag="ACG8KQ_000834"
     CDS             7999..8502
                     /locus_tag="ACG8KQ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP9103796.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8462..8800
                     /locus_tag="ACG8KQ_000835"
     CDS             8462..8800
                     /locus_tag="ACG8KQ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9103797.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8821..10239
                     /gene="ffh"
                     /locus_tag="ACG8KQ_000836"
     CDS             8821..10239
                     /gene="ffh"
                     /locus_tag="ACG8KQ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9103798.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10351..10929
                     /locus_tag="ACG8KQ_000837"
     CDS             10351..10929
                     /locus_tag="ACG8KQ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9103799.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10933..11454
                     /locus_tag="ACG8KQ_000838"
     CDS             10933..11454
                     /locus_tag="ACG8KQ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9103800.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11533..12087)
                     /locus_tag="ACG8KQ_000839"
     CDS             complement(11533..12087)
                     /locus_tag="ACG8KQ_000839"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9103801.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12340..12615
                     /gene="rpsP"
                     /locus_tag="ACG8KQ_000840"
     CDS             12340..12615
                     /gene="rpsP"
                     /locus_tag="ACG8KQ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9103802.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12627..12878
                     /locus_tag="ACG8KQ_000841"
     CDS             12627..12878
                     /locus_tag="ACG8KQ_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9103803.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13003..13794
                     /locus_tag="ACG8KQ_000842"
     CDS             13003..13794
                     /locus_tag="ACG8KQ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103804.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13964..14485
                     /gene="rimM"
                     /locus_tag="ACG8KQ_000843"
     CDS             13964..14485
                     /gene="rimM"
                     /locus_tag="ACG8KQ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9103805.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14475..15236
                     /gene="trmD"
                     /locus_tag="ACG8KQ_000844"
     CDS             14475..15236
                     /gene="trmD"
                     /locus_tag="ACG8KQ_000844"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9103806.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15372..15719
                     /gene="rplS"
                     /locus_tag="ACG8KQ_000845"
     CDS             15372..15719
                     /gene="rplS"
                     /locus_tag="ACG8KQ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9103807.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15851..16987)
                     /locus_tag="ACG8KQ_000846"
     CDS             complement(15851..16987)
                     /locus_tag="ACG8KQ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9103808.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17103..17669)
                     /locus_tag="ACG8KQ_000847"
     CDS             complement(17103..17669)
                     /locus_tag="ACG8KQ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFP9103809.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17805..18458)
                     /locus_tag="ACG8KQ_000848"
     CDS             complement(17805..18458)
                     /locus_tag="ACG8KQ_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103810.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18508..18936)
                     /locus_tag="ACG8KQ_000849"
     CDS             complement(18508..18936)
                     /locus_tag="ACG8KQ_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9103811.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18941..19315)
                     /locus_tag="ACG8KQ_000850"
     CDS             complement(18941..19315)
                     /locus_tag="ACG8KQ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9103812.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            19619..19804
                     /locus_tag="ACG8KQ_000851"
     CDS             19619..19804
                     /locus_tag="ACG8KQ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103813.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            19817..20065
                     /locus_tag="ACG8KQ_000852"
     CDS             19817..20065
                     /locus_tag="ACG8KQ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103814.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            20096..20266
                     /locus_tag="ACG8KQ_000853"
     CDS             20096..20266
                     /locus_tag="ACG8KQ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103815.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(20368..20835)
                     /locus_tag="ACG8KQ_000854"
     CDS             complement(20368..20835)
                     /locus_tag="ACG8KQ_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103816.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            20994..21311
                     /locus_tag="ACG8KQ_000855"
     CDS             20994..21311
                     /locus_tag="ACG8KQ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103817.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            21244..21723
                     /locus_tag="ACG8KQ_000856"
     CDS             21244..21723
                     /locus_tag="ACG8KQ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFP9103818.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            21740..21916
                     /locus_tag="ACG8KQ_000857"
     CDS             21740..21916
                     /locus_tag="ACG8KQ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103819.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            22173..22604
                     /locus_tag="ACG8KQ_000858"
     CDS             22173..22604
                     /locus_tag="ACG8KQ_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103820.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            22610..22927
                     /locus_tag="ACG8KQ_000859"
     CDS             22610..22927
                     /locus_tag="ACG8KQ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9103821.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            23046..23732
                     /locus_tag="ACG8KQ_000860"
     CDS             23046..23732
                     /locus_tag="ACG8KQ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9103822.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            23707..25056
                     /locus_tag="ACG8KQ_000861"
     CDS             23707..25056
                     /locus_tag="ACG8KQ_000861"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9103823.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            25047..25520
                     /locus_tag="ACG8KQ_000862"
     CDS             25047..25520
                     /locus_tag="ACG8KQ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9103824.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            25522..26022
                     /locus_tag="ACG8KQ_000863"
     CDS             25522..26022
                     /locus_tag="ACG8KQ_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFP9103825.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            26037..28349
                     /locus_tag="ACG8KQ_000864"
     CDS             26037..28349
                     /locus_tag="ACG8KQ_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFP9103826.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            28596..28913
                     /locus_tag="ACG8KQ_000865"
     CDS             28596..28913
                     /locus_tag="ACG8KQ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP9103827.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            28910..29071
                     /locus_tag="ACG8KQ_000866"
     CDS             28910..29071
                     /locus_tag="ACG8KQ_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9103828.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            29074..29373
                     /locus_tag="ACG8KQ_000867"
     CDS             29074..29373
                     /locus_tag="ACG8KQ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103829.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            29373..29687
                     /locus_tag="ACG8KQ_000868"
     CDS             29373..29687
                     /locus_tag="ACG8KQ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9103830.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            29680..30210
                     /locus_tag="ACG8KQ_000869"
     CDS             29680..30210
                     /locus_tag="ACG8KQ_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9103831.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            30207..30476
                     /locus_tag="ACG8KQ_000870"
     CDS             30207..30476
                     /locus_tag="ACG8KQ_000870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103832.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            30406..30609
                     /locus_tag="ACG8KQ_000871"
     CDS             30406..30609
                     /locus_tag="ACG8KQ_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP9103833.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            30606..30893
                     /locus_tag="ACG8KQ_000872"
     CDS             30606..30893
                     /locus_tag="ACG8KQ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103834.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(30926..31177)
                     /locus_tag="ACG8KQ_000873"
     CDS             complement(30926..31177)
                     /locus_tag="ACG8KQ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103835.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            31380..31793
                     /locus_tag="ACG8KQ_000874"
     CDS             31380..31793
                     /locus_tag="ACG8KQ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9103836.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT        10786 a         5495 c         6571 g         9123 t
ORIGIN      
        1 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
       61 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      121 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      181 aaatagaaaa actagtgata gttttggtga tagtgatatt aaaaatcaga aaagatagaa
      241 aaagcaggag atagataaaa tgctgatatt tcagcatttt ttaagagtat aaaagagaaa
      301 aaaagtaata aatgtataca tgatataaga aaaacatata tgtataaaaa gaagaaagta
      361 gcttctcttt gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag
      421 gctaccgccc ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag
      481 atcagctggg tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa
      541 aataaatagg aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg
      601 ttcctttgaa aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt
      661 acggaatgat aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa
      721 agcaacggaa ggtttttcca gcaagttttt atcatctttc atcaccacct aacaagtgtt
      781 attattttgt tagttttatt gtaaattgaa tcctgcaata atcaattact ttttcattat
      841 ttctagatta aatgaataaa ctctcatgcc attatctcta aaaaaaaagt ttttccccct
      901 aattttttta gatagaaaat tgtatagtat gatgagagaa aaagtagttt gggggaacgt
      961 taatgagaag aaaagcagta atcgatcggc tttatttatt tttttggttt tttcctatcg
     1021 ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg tgcggagata ttaggcctga
     1081 tccaaggaag cggatttgtg gtttctagga attttccagc tttgactgtt ttctatttag
     1141 tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc
     1201 tttacttttc ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta
     1261 tcggccctaa tggtttgagc gagagtggat tgaaggattc agcttattcc tccatgtatt
     1321 tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt
     1381 tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact
     1441 attgatggtg gatcatcagt aaaaaagcat atgagctgcg taaaatgcat gtttctcata
     1501 tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt
     1561 aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt
     1621 aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt
     1681 cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat
     1741 tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg
     1801 ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta
     1861 caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga
     1921 aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat
     1981 aaagtagcag tggtcgggat ggcgccaatc gtcaataaag atacagatgt caaagatatc
     2041 accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc
     2101 gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca
     2161 ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca
     2221 ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca
     2281 tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat
     2341 gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct
     2401 tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct
     2461 gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga
     2521 aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca
     2581 actatgtttc ccacatatac atttttcctt taattgtgaa aaatagttcc tcatattttg
     2641 ccgttttttg agctaatacc gagggtcagc tgattaaaca ccgtttattc gaaaaaaaga
     2701 gctacgacat agaactgtcg tagctctttt tttgctatat gacaggaatg aaaggtgttt
     2761 gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg agaagtttgg
     2821 aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg gataaatcga
     2881 agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct ttttccatcg
     2941 tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat
     3001 aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa catatttacg
     3061 tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga atcctataag
     3121 aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt tcaggaaatt
     3181 cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt caatgtcata
     3241 cgggacggga tagaacaaac aatagatata gaagaccttg taatagatga cattgttatt
     3301 ttagagactg gacatcaaat tccagcagat gcagaggtcg tggaaggaaa actgcaagtt
     3361 aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg cgatccatta
     3421 atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa agttggagca
     3481 gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg agaacagtcg
     3541 gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat tattcctatc
     3601 ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa agaaagtgtc
     3661 gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt gctgacgacg
     3721 attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt acacaatatg
     3781 aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac aggaacgatc
     3841 acagaaaata aaatgtctgt tcaaaaagtg atcgtctcta aaaaacaaga aataatcagt
     3901 cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc aggagacaat
     3961 gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc ctatcaccat
     4021 gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga caaggtgtat
     4081 gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc ggaagagttt
     4141 gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt
     4201 gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat tgctaatcct
     4261 atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt agccatcaaa
     4321 gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc agggatttca
     4381 catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga agcagctgtc
     4441 gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg
     4501 ataaagcgta acaatacagt agctatgaca ggtgatggcg tgaatgatat tctagccatg
     4561 aaagaagcag actgcagtat agcgatggct tccggaaatg aagccgccat gcaggcctca
     4621 caagtggtct tacttgattc tgacttttca cgcatgccag aagttgttgc agaaggacgg
     4681 cgcgttgtga acaatattga gcgatcagcc agcttgtttt tagtgaagaa tattttctcc
     4741 tttttgctct cactgttttc agtgatcttt gctctgacct atcctttaga accatcacaa
     4801 atcacattga ttagtttgtt tactatcgga cttccatctt tcttgcttgc tctagaagaa
     4861 aacaaaaagc gaatcagagg gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt
     4921 ggactaacag atatgatcgt agtcggtgcg ttggtgatct gcggggtgac tttgaatcta
     4981 aataaaacag atgtttctac agcatctacc atgcttttga ttgcagtagg gttcctcgtt
     5041 ctctataaaa tctgttcacc gctgaataaa ttcagaagtc agatcattct tttctgtgct
     5101 agcggtaact ttttttcggt catctttttg cataagctgt tttcaatcac cggtatctca
     5161 gctgtatcac ttttgctgtt gagcattcta tttttctcgg cagattcgat ttttaggcat
     5221 ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt
     5281 agtcgcgtag ctaaaaaatt tctatcgata tttaaacgag aaaattgacg aaaagtatct
     5341 caaaaaagag taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca
     5401 actttaaaac gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt
     5461 cgaacctgtt ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc
     5521 ttttacaatc gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac
     5581 gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc
     5641 ttggacagaa aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct
     5701 ttttgaatat tggacagcat tttcaatcag atttttgata attggataaa ggatatctgt
     5761 tttagtagta aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat
     5821 gattggctga taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat
     5881 ctcgtaagaa ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa
     5941 ccggatcgca tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt
     6001 tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc
     6061 gtgagagacg ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa
     6121 gtcatacgcc acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact
     6181 caagtctaat gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc
     6241 aggagtcact tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg
     6301 ctgttcaggt tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag
     6361 taaacgagaa tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt
     6421 atataattgc tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa
     6481 cgcattaatc gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc
     6541 ctgattagga ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt
     6601 tttttgatag atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa
     6661 gaaaaagacc caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc
     6721 gatacgtaaa atggcttcta actggccact ttttttgact ggtaatgcga catataaaag
     6781 atcctcattc aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac
     6841 ggctttgatt tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa
     6901 taagatcttt ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc
     6961 ttccaaagca gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag
     7021 cagttgtgct ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata
     7081 atgggaaatc aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag
     7141 acgttcataa atttttttcc atttcatcgt tttggttcct gaaacttata gccaaatcct
     7201 ctaatagtca ccaagtactt tggatgtttt ggatctcgct cgattttttc tcgcaaatga
     7261 ctaacgtgta catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct
     7321 agcaatgtgt ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca
     7381 aattctttcg gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa
     7441 tcagcagtga tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta
     7501 cgaggttcaa tccgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat
     7561 ggtttggtca aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca
     7621 tctttagccg tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg
     7681 atctccatgc catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt
     7741 tctaatgcta attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct
     7801 ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt
     7861 tttttcattc aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca
     7921 gtacaaaggg ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat
     7981 agaaaggaag agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc
     8041 tatgatggac agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct
     8101 gtcatggtct gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc
     8161 attggtggaa atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc
     8221 gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa
     8281 aaacacattg atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt
     8341 tatgacggaa gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt
     8401 attcagcaaa aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag
     8461 aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt
     8521 gacagaaaaa caaatgaact acatggaatt atattatgca gatgattttt cattaggtga
     8581 gatcgcggaa gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag
     8641 caagatcttg gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga
     8701 acaggtattg gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact
     8761 acttgaatat gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat
     8821 atggcatttg aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa
     8881 ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta
     8941 gaagccgatg ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg
     9001 gtaggggcag aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat
     9061 gaagaattga cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata
     9121 ccaactgtta tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa
     9181 ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta
     9241 tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta
     9301 tttgatatgg gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca
     9361 aaagaaaaga aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa
     9421 acattgatgg atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt
     9481 gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa
     9541 ttaggtatta caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca
     9601 ctatcgatcc gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg
     9661 actgacttag aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat
     9721 atgctgacat tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt
     9781 gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa
     9841 gtgatgggta tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc
     9901 cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt
     9961 tattctatga cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc
    10021 cgaattgctg ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc
    10081 aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg
    10141 gaccaaatgt ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg
    10201 atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt
    10261 acagactgaa aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat
    10321 acaattactc taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa
    10381 agtatgcggc aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg
    10441 ttccgaaaat tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat
    10501 aaaagtgaag cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat
    10561 ggagaaacaa aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa
    10621 atcgaagcag ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt
    10681 ttttcagagt atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca
    10741 caagaagaag tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa
    10801 agaggattta aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc
    10861 atcaatgatc accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag
    10921 ggcgaataaa gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg
    10981 gcaacgaaaa cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact
    11041 tatgaagctt tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa
    11101 gcaaaaaaat atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc
    11161 acccacgcac aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt
    11221 ctaaaaaatc tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg
    11281 atcaaaccgc tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta
    11341 ttattagggg acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg
    11401 ctagacacga cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa
    11461 ataaaataaa aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta
    11521 ccgttatgct ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt
    11581 ttggaagaaa cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac
    11641 gtttccgata tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg
    11701 gcgtagatgg tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa
    11761 acctggttta tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt
    11821 tttcaatact gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag
    11881 tcccctcact tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag
    11941 atcctcattg taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg
    12001 aagcaatttc gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc
    12061 aataaaaaat ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa
    12121 tcttacaaaa gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga
    12181 agatagatta cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa
    12241 tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac
    12301 tacatcttgt gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta
    12361 aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct
    12421 cctcgtgatg gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca
    12481 gaagtagttt taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct
    12541 gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa
    12601 ttctcaaaga aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg
    12661 ttcgtccgct agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact
    12721 tttacgagta caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag
    12781 ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa
    12841 aagtgcgttt gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt
    12901 ttcgagtgca gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa
    12961 gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta
    13021 tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat
    13081 acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa
    13141 acggcttcca ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta
    13201 gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca
    13261 ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga
    13321 acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc
    13381 tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt
    13441 gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa
    13501 ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt
    13561 gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg
    13621 gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat
    13681 acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact
    13741 catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag
    13801 cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg
    13861 ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac
    13921 acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa
    13981 tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac
    14041 ggatttccca gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa
    14101 agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag
    14161 ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt
    14221 gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg
    14281 tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg
    14341 tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat
    14401 cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga
    14461 aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc
    14521 ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct
    14581 ctaacttccg cgattattca gataacaaac atcagacagt agatgattat ccatacggcg
    14641 gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa
    14701 aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg
    14761 atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt
    14821 atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt
    14881 atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt
    14941 tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat
    15001 tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat
    15061 tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga
    15121 cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat
    15181 ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt
    15241 ttctaaaatc ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa
    15301 gcacttaact attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg
    15361 agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca
    15421 tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc
    15481 gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg
    15541 aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc
    15601 atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat
    15661 tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat
    15721 catcgttatc agacagagaa agaacccttg gtattcaagg gttctttttt tattatctgt
    15781 ttttttatat taacgatttc tatttggtac atttttgatt aataaaaagg ggcaaaaaag
    15841 gggcaacaag ttacagtttt tctatatctt ctttcaattt ttcttttact ccttttgtca
    15901 catgcaaata aattttttca gtgatatcac tattttcgtg accaacgcga tcctgaatgg
    15961 catacagagg agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg
    16021 aactaagttt tttatcaatc ttcatgtcag ctttatgact tctcaaatag gtgttaattg
    16081 ctgttagttg gaaaggagtt cctttcgttg tttgaaatag aaattgacca tttggattta
    16141 attctagaag ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc
    16201 cggcagctgt ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca
    16261 tcatcgtccc atttataact actgatgcat tattattggt aatgtgtaca tcatctttac
    16321 ttaatgcgat tgcttcacca gacctcatcc cagtcaaata tagccactgg aaaaggagag
    16381 aatatctttt attgtgtgaa gttgtgaaat ctactagtct attatattca tcatcttcta
    16441 aaaatttatc tttaattttt atcgtttttg actctctttt ataatcaata actacttcat
    16501 cgataggatt tttttctaca taaccttttt tcatagcata tgaaaaaaga agattcaatt
    16561 tggatttaat tacactgacg tacttatttg acaagtcatc tttatatatc atgtcttcaa
    16621 aaatattatt caaatctatt gtattaatac cagaaacgat gtaagtctca ggtatcttct
    16681 tctttatagt gtttaatata ttattcgtag gataatatgt agattcctta acttgtcttt
    16741 tgtatataac taaccattct tcaacaagct catgaaatgt tagatcaggc ttatgaagtg
    16801 ttttttcgtt taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag
    16861 tttctcgaga tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat
    16921 caatatatcg ctcattatat ttatatactt tttttccgtt tttatcagtt ttagtttcga
    16981 tccacattgt tttcatctcc tatttgttgc tataatagga atagataagt aagccgatta
    17041 tagcaggttt atttttcatc acgtccacaa actttggcga gggaggggcg tgtttttgtt
    17101 agttattgtg gtaggttatc taaagcgtat tgtgcttgct catttgtaaa accttcaaat
    17161 ataagttgat cgtacaattg cgaatctgac attgatgccc aatcatcgta actttccgct
    17221 ttagccaaag cttgttcgtt gtaatcgaca tcaacattag ccagcgcata atcgattgca
    17281 tcacttggat attcttcaaa ctctagttgt tcacgcaaac cttgttcaga cataccagcc
    17341 caacctagat agctttcagc tttgctcaat gcatttctgt attcacgagg aacactttct
    17401 tcttgagtaa cttcttctat agtagtttca gtagtttcag tttcttcttc aacgactgta
    17461 gattctgcta ccgattctgc agaagacact tctgtcacgc tttctttagt tgtttcagcg
    17521 acggtatttt tagcatcatc actgcctcct tgcgaaccta tagcaataaa caccacaaca
    17581 accgctaaca accaaaacca tacccgttta taaaacggtt tcttaacttt atacattttc
    17641 ccatcttgac ccataacttt ttttgccatt taaatatccc tcgcttcttg ttataatata
    17701 tttgtgatct cagaaatgag gtatgagtcc gtgttgcagc acgggctttt ttctttataa
    17761 cttttttaga gattataggc aaaatagtag ggcataaaaa tgtattattg aattccgtat
    17821 ttagaaaatc ctaattgaac ttcaccggaa gtcttttgct gtgttgtacg caatgcttct
    17881 tcttcagaca ttccattctg tactttccat gcaacaggcg acatcccgta tttgttaaca
    17941 aaatcagtaa gtgataaagt gtcagcgtct tgctgagcgc ttgtttgttg gtcttctgga
    18001 ttctgttgag atgctgcttg ttgttctttc tgatcttgac tgataatatt gccagcatca
    18061 tctgtagtca atccattttc ataaagggcc acgccgaaag cttcccactc tttgttggac
    18121 caatttgcac gatcagctgg agttgactgt aaagtgcgtt gtttcatctg ttcatatgtt
    18181 tcttcttgag gtgcggtttg gattgtactc tgactggagc ttataactgt tgggctaggt
    18241 tccgccgtag cttggttgga gcttgtaact gttgaactag aatctgtttt agatgtagat
    18301 ttgctagtag atgaactggt ttcagttgtt tcttttgttt tacttatttt tgtttcttgg
    18361 ttagaagtgg tatctgttga ttcagctttt ttattatttg aacaagctga aagtagcaga
    18421 gcagtactta acaacaacat aacgctaact tttttcattt tataattcct ctttctcgtt
    18481 gtaatatgtg tgctaacacg ggctttttta tataagaaaa cgataagcgc tttctggaag
    18541 cccgtaaaga ttctttaatt cctcgatttt tttaggatat tgatcattat cttctttata
    18601 aagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt tgcttctaga
    18661 tgttcttgtt gatacataat agctggataa gccacgatga aaaatagcgt gacctaattc
    18721 gtgagcgcaa atgtagaatc tttcctcaga gtctcgcagt tcatcattta aaaagattat
    18781 cgcacgatct ctaatttctt gaaactgccc cttgggattc tcgataaaag gaacgtattg
    18841 aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt atttccgctt
    18901 caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt ttgccttttt
    18961 ctttgtcttc tttcacaata tcccagaaag tcgctatcag gatatctttt acgcgctgta
    19021 tttgttcggg tgtcaatgtt tccccaccat aagacatatt aacatttgag tctagtagtt
    19081 tatcaagttc aaccacttcc tcttttgtag cccacttggg aacattatta tttcccaata
    19141 aataatcagt tgtgacacca aaataatcag caactttctt taagttctca gattttggcg
    19201 aggctttatc ccatcttctt atttgtccat tagagatgcc cacctgtctt tctacttctg
    19261 ctatagtcac atgcttttcg tctgctaatt ctttaatctt agtaactaaa ctcattatta
    19321 tcaacctttc aaagctgaaa agaaaataaa tagcttaaaa gttatatttt tggttgacaa
    19381 ttagctttta agctagtata tttaattcgt aagctaaatt gttagctaaa taagagcaac
    19441 aaaaaactct actaatttaa aacattctct cggtcgccaa acttagaaat gttattttag
    19501 aggcttttta taagtcttat ttaactatgt attcataata gcttaaaagc taataggtgt
    19561 caacgattta gctaattttt tagcttacaa attatttgtt tagaaaggag ctatttttat
    19621 gtctgagaat ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa
    19681 agaactagct gcgcttatcg gtatttcagg agcttattta tcagatattc taaacggcaa
    19741 tcgtgatgga aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg
    19801 ataggggggg taggcgatga caaaactaaa aaaacaagat tttgtaaaaa aatataatta
    19861 ttctccatct acttatcaac gtcgaatgtc ggaactaaaa aatacagcaa ttttctcagc
    19921 ggcgtatgaa cgggtcacag gacaagaagt ttggatcaat acagaattat acgataaatt
    19981 tttgtctttc aaatcctata acaggttacg cacaagaaag gtaacaccta aagaatttat
    20041 cgagaagcat ttagttgatt tataaaaaac agaacatttt gagaggtgaa ggttaatggg
    20101 taaattcaac agagcattag tattcagcgc accgctaatc gtctatgctt tagggctttg
    20161 gggaagtaga caagcgttga taggaacgat tgtgtacatg gtttggattt ttatagggct
    20221 ggatgaagct gagtacaaaa caaaaaagcc agtcgggagg gactgactaa tgagtaagga
    20281 cgatattgta tttctgttaa ttatgttttg tgggattgga tcaataataa ttaatttgta
    20341 tttgattatt aaagaaaaat ttttcaatta aaatgagtct tttagcttta acttaaattt
    20401 tttttgataa ttagctgttc ttatgaccat tacaagtttt tcaccaggta atatatcttt
    20461 catatctaaa acttctgaaa aagcgactga gtagccagga gagattgctt gtgccaacgg
    20521 tataggagat aaaaggctac ttttatgaat gcttcccata tcaaaaccat tggcgtgata
    20581 atgtgaacaa ttaattttct taccgttgta aagatacgta cattcgataa ttgagtttgg
    20641 catttttgat gggtttgcaa ttacgccagt aagaatcatt cgattacttc tatcatgctt
    20701 aactaaaaaa taggagagcg taatttttct tttatcttta aataagttat agagggataa
    20761 aaaaaatcct gaaatagaaa tgattaaagt aatagcgact tggtaatcgc tcaaaatttt
    20821 tatcaagtaa ttcaccacct ttttactcaa attatatcaa aaaggagaga agaaataatg
    20881 caagaattag taattttgaa aaataaaaaa agacacgaac gccggcaagc atgatcgtgt
    20941 cagggaaata aactatcttt ctatatttta ccacagaaag gaatgaaccg taaatgctaa
    21001 ttgcaacgga tacactagac cgcatctttt taaaagacga atacaaactg cgcaatatag
    21061 atgcgtcagg aattttagta tttgatcttt atgacaatgg aaaaattggt atctatcaag
    21121 caagtgatat tgaagaaaca aaccttgctt tcgaacaaat tgatgattct gtggaattgg
    21181 atttagatga ggcaatccta gcttttgaac aaattgcaaa attattaaag gaggcacaaa
    21241 agaatggcaa ctctttacca actcagcgag tcatatatca aagtcctgga actggcagaa
    21301 caattggatg aagaaattct tcgcgatact ctcgattcga ttaatgaagc gatcgagtat
    21361 aaggcagaaa acttagcaaa aatagttaaa gaagtagaag ggaaagctga gttaatcgat
    21421 agtgaaatta aacgtctgca ggaacgtaag acatcgcttt tgaacaatgc taagagtatc
    21481 aagcactatt tacaagagga aatggaaaag actggtaaaa cgaagattaa aggtgaatta
    21541 ttcaacattg gaattcaaaa taatccagta tcggtcaatg taatcaatga gaacttaatt
    21601 ccaaaaggat tctttacccc tgtgcctccc aaattggata aaaagcaatt gaaggaggaa
    21661 ctgaagcacg gagatattcc tggtgctgaa ctcgtccaaa caaaaggttt gaggataaga
    21721 tgatgaagga ggagaaataa tggaacacga aaacgtaaaa gacgctttga aagctgctat
    21781 cgaaatcgcg gaagctaaag gaataaaggt tgacggcaaa ccagcaactg tccatgacat
    21841 tcaaaatttg acgaaagaac atctttattt tatcgccgac ttgcttggtt tgtcggaatt
    21901 gtatttagac aaataacccc acacggaagg agtggctagg gtggttgtat tacaagtaat
    21961 agtctgcgta acattcattt ggtcgtgcat accggtttgt atatatatca tgaagaagct
    22021 tcgccctgag tcttacaaat tatacgcaat ttacagcata acagtagctc ttgcatttac
    22081 attgtttgca atttctaggc ttccgtttta atcgtgggaa tgaattttta atgaactaaa
    22141 ttatctataa agcaaaaaaa ggagaaacaa cattgaaaaa caaactagca aaaacaacag
    22201 caatcatcgg actagcacta ggtagcggcg ttatcggcta cgcagcaagc aatgcgtttc
    22261 aagatttgga cacgatcaaa gcaaatttca acacagtcct acaatacggt caaacgaaat
    22321 cgcaacgtgt gtcagaactt gaatcacagc tatccaacaa cactcgcaca caggagcagc
    22381 tgaaagccga aattgagcaa atcaaatcgg acaagcagaa ggaaattgaa gccaagcaac
    22441 gagaagtcga acaaaagcaa caggagatcg ctacaaagca acaggaagcc gatagcttgc
    22501 gccaacaact atccacagtg caaaacgaca aggagcagtt agaacagcgc gtgagcgagt
    22561 tacggcagta tacggatcaa aaggtagggg agttggggaa atgaaagagt tgactattta
    22621 tgacccaaat atttcatggg ctaaacacac aattaaagtt tcttttatga tttggggtta
    22681 caaaggatat gttacttaca aagtgggcgg taacactaag ggtttatcat taatagctat
    22741 tgattcagat gatctatatg atgctaattt cgaggataac ccagttaatt tccgtgacct
    22801 tgatgaagat tggttctcta tggaacttac taacgataaa ggcgacagta cacttgtaga
    22861 agatgaattc gacagactag gtgattatat tgtcggtgtt gaaattattg cgcacgaacc
    22921 agagtaggag agtgaataca aaatgattaa aagaatcact atatcagttg aagaagatag
    22981 tggatcaaca atgcagcata ccgacaatcg aaaaactttt ttaaccaata gaaaggagaa
    23041 ttttgatgca gattaagaag gcgacggata tcgaacaaac caagggaaca tatttgatct
    23101 acggtgctcc aggaaaaggt aaaacttcaa cggctaaata ttttccaggg aaaacactga
    23161 tccttgatat tgatcggact tcaaaagtcc ttagaggtga gaaaaatgtc gatatcgtat
    23221 atatcgacaa cgaagatacc tggaacgact ggggcaacac gttggctgat ttaactacca
    23281 attatgtagg tgtttatgac aacgtggttg tcgataatgt cagcgaattg gaacggtgta
    23341 ttctgtccag tttaggcgca gaaggaaaaa acaacggcgt tccttcgcaa ggcgattatc
    23401 aatatatgca atttagaatg gtgaacagcc tacgctatat gaaaaattta gacagcaacc
    23461 ttatctggac cgcatgggaa gaaatcgatt tgtggacgga ttcggatgga tcttcttaca
    23521 acatcgcttt gccgcagatc aatcgaaaga ttcgaaacaa tattttggga ttatgtgacg
    23581 ttgttggtcg attgatggta aaggaagacg atgaacgcgg atttatttta caagccacag
    23641 attcaaccta tgccaaaaat caattggata atagatctgg ctgtaagcaa agtgagctga
    23701 ttttagatgg aattgtacga ctaccagaat gatttagtcg atcgtgcaag acaagcctat
    23761 gttgatggtt acaaggcacc atgtatcgta tcaccgtgcg gttctggaaa atcagtcatg
    23821 gttgcagaga tcgctaaacg aaccacacga aaaggcaaca gagtgttgtt cttggtccat
    23881 cgtcgtgaat tgattgatca gatcaaagat acgtttcaaa agatgggcgt agataatcag
    23941 cttgtaaatt tcggcatggt tcaaacagtc gtacgacatc ttaaaaccat caagaaacca
    24001 caactgatca tcactgacga aaaccatcac gggttagcag cgtcctacag aaaaatctat
    24061 gagtattatt cggacgttcc gcgattaggt tttaccgcga cacccatccg gttaaacggt
    24121 agtgggttgg gggatgtgaa cgacttgcta attgaaggag tatcggctaa atggttgatt
    24181 gagaatcata gattagctcc ttatgaatat tatgcaccta aattgatcga tacagccgaa
    24241 ttaaagaaag cttcgactgg agattttaca aagaaatcaa tggataaggc agtgaagaat
    24301 actatttatg gcgacgtgtt gaaacattat aaaacgttag cagaaggtga acaggcaata
    24361 gcatactgtc acagtatcga tgcaagtaaa cacactgctg aaatattcaa cgatgctggt
    24421 tataaagcaa cacatatcga tgcaaaaact ccaaaagatg aacgtgcaga tattatcgaa
    24481 gcgttcagaa atcatgaaat taaaatccta tgcaatgttg atttgatcgg agaagggttt
    24541 gatgttcctg attgttcgac tgtaattatg ttgagaccta ctcaatcgtt atcattgtac
    24601 attcagcaat cgatgagggg gatgcgttac cgaccagata aaacgtcaat catcattgac
    24661 catgtgggta atgtgaaccg ttttggattg cctgatatgg atcgtgaatg gagtttagat
    24721 acaaaaaaga aaacaaaaac tgatagtgat ttatctatcg ttcaatgcgc gttttgtttt
    24781 ggtgcttacg aacggccaaa aggcgataat atttgtccgt attgcggaga gttgcagccg
    24841 attgaagaac gaaagtcaga gttagaaatc gatgaatcgg ctgaattaat gaaagtcgga
    24901 gaaacgaaaa ttactttgaa ttttgagaac aataaatatt acaacatgac ggaagatgaa
    24961 gcagaaagta tagaggactt atatgccata gcaagagcta aaggatttaa acctggttgg
    25021 gcttatatgg ctgctaagag gaaggggtgg ttgtaggaat gggcggtatt tttggtaaaa
    25081 aagcagaaga tgttcaaggt tcaataataa atggcgtaac aataataggt gatactggaa
    25141 aaagagatag ttcaggcaat caaattgtaa tcgctagaaa taaaaataac gaagtttttg
    25201 aatcttctct atctaatata aaaagaggtg ttgttaacgg gacaggcgaa gagagtggct
    25261 acgttaattt aaaaaaagct aatgaaaaat accatgttga aggaacttac gttaaggctt
    25321 tgtctgtcgg tggaacaaaa ggcgttaatt gggataaacg aagaaataaa tggaaagcac
    25381 gattatattt caaaggagaa tatgtcttac aaaaggattt caacaccaaa gaagaagcaa
    25441 tccaagcacg acttgcagca gaagaaaaat atttcaaacc tatcttagaa aaatatgaaa
    25501 ataaaaagga gaatgattaa catgacagga tttaacttag acttttcaaa agcgcaacaa
    25561 gggaacgaaa tcaaagacgg tacgtacgaa gtggtcgtaa acaaagctgt agaaaatgca
    25621 actaaatcag gcgcagaatt tatcgatatc gatttgatcg ttcgtaacga tgtggatcaa
    25681 ccgttccaaa ataaacacat tttcgcaaaa atctggaaag ccaaagctac cggcaaatat
    25741 aacgaaggta tgatcatggc aattgcacaa gcgctacagt tggaagacgg aaaatcatat
    25801 aacgggtttg atgaattgct ggcggacttt gttttgaaaa ccgcctcagt acgcgtgaaa
    25861 actgaagagt caaatggata taaaaacgtc aatgtgaagt cttgggataa aacgaacaca
    25921 cggggcgtaa tgaatcacca attcaaaaat ggtgatgaac catccttcgg accggaacgt
    25981 tcaagagcga caacggttag aaatgacaat cttccatttt aattgaggtg taatcaatgt
    26041 atgaaaaaat tccaaaagag cttaaaaatc tcaaacaatg gtgtgtttat aagctagttt
    26101 gggatgaaaa gcggcagaag cacacgaaga tcccttataa cgccaacaac gggcataaag
    26161 ccaaatccaa tgacgagagt acctggtctg attttcagac cgctctcgct gctattgatc
    26221 actacggaat gagtggactg gggttctttt tcaaaccacc ttatttcggc attgatatcg
    26281 ataatgctga aggtgaagtt gagcgattta aatcaggtga tatagaagaa aatattatct
    26341 atgaatttat cgaaagcatg agatcctatg ctgagtattc tcaatcaggt actggcatcc
    26401 acattattgc tcgcggagaa ttgcccggcg gaagacgcag gaaaggtgac gtagaaatgt
    26461 accagaacgg ccgattcttc gttatgactg gaaatgccgc atcaaaatat ttagaaataa
    26521 ctgagccgaa tccaaaggat atcaaacgcc tatatgatcg ctacgttgga gatgaaaaaa
    26581 tcatccaatt caaagaagaa aatccgctga tgaataccgt tgatttaccg gttgaagaaa
    26641 ttatcaaacg tgctgaatcg tcttcccaag gggcaagatt caaagtcttt atgaacggcg
    26701 gttgggaagc tttttattcc tcgcaatccg aagcggatat ggcgttcgcc aacgacttgg
    26761 ctttttggac cggtagagac tttgaaaaaa tggatgagat tttcagagcg tctgcaatga
    26821 tccgtagcaa atatgaccaa aaaaggggat cggcaactta cggagaatct ctactaaata
    26881 aggcaattgc tgatacaaat tccgtctaca accccaaacg gaaatctgat tttaagattt
    26941 ttatcaaaga ccaggaacag ccaaaagagg aaaaatacta ttcgtacgac gacaccggca
    27001 acgcggatcg gtttactgat atctacggaa cgttagtcaa atattcgtac attgataaat
    27061 cttggtatta ctacgatggg aaagtttggc tacaggacaa taccggcgaa gttcggaaaa
    27121 tgatcgatac gacagtagac atcatgggaa aagaaccgtt aacgatccct gaaggcgcag
    27181 acgatgaaac caaagaagct ttgatgaaag cgaaagaaaa acacgtcaag cgttctcgca
    27241 gcaatgccgg aaaaaacgct atgatggacg aattgaaaca tcgactatcc gtattgccgg
    27301 aagagttcga caaagataaa acgctgttca acacacaaag cggctatctg tctttgcatg
    27361 acggtcttct acacgagcat gagatagaca aaatgtttac acgtgtttca aatgtcgagt
    27421 acacagaatc ggtcgattgt cctatgtggg aagaatttat taatcagatt tttgacaatg
    27481 atcaagaatt aatcagatat atccagaaat gtgtcggata ttccctgacc ggatctacca
    27541 gagagcaatg tatgttcatc ctctacggtc atgggtcaaa tggaaagtcg gtatttttgg
    27601 aaattatttc tgaattgatg ggaaactatg cgatgactat gcaggcacaa acgatcatgg
    27661 tcaagcaaag ccaatcatca gcaaacagtg atattgcacg attgaaaggt gcgcggttgg
    27721 tcacttcgtc agaaccgaat gaaggcgtcc gactggacga aggtctggtc aaacaattga
    27781 ctggtggtga taaagtcacg gctcggcacc tttatggaaa agaattcgag ttcgaacctg
    27841 aattcaagtt gtggctggca acgaaccaca agcccatcat ccgtgggacc gatgacggca
    27901 tctggcgacg gttaaattta attccattca ctgtccaaat cccggatcac aaaaaggata
    27961 aaaatctgaa atttaaattg cagacggagt tgcaagggat ccttaaatgg gcaatcgatg
    28021 gttgcctgtt gtggcagaga gagggcttgg agaaaccaaa gtcggttgtt gcagcgagtc
    28081 aggattatcg aaacgaaatg gatcaaatag gtacatttat cgaaacgtgt tgcgaaacag
    28141 gtccaggact aaaaatatca ggcggagaat tatacaaagt ttatcgtgaa tgggcatcag
    28201 ataatggcga gcatactttc actaacacga agtttggcag agagatttca aagaaatata
    28261 gcaaagaaaa agctggtgga tttatggttt ataaaggaat cacattaaaa ccaagaaaat
    28321 acgataacgt tagagaactt tttaaataaa tctggagggt tctggagggt tgagaacgaa
    28381 accctccaac ccttagaaac gttgatatat cagtatttat attatatttt tctttctttc
    28441 tggagggttt taaaaaaata ataaagtatt aaagtaaaaa ataaaaagta aattatataa
    28501 aaaagaatag gtttttgggt ctgaccctcc aaactctcca ccaaaatctt cctaaatgct
    28561 ttgataccaa cgattaaaca gtctggaggg ttacattgaa atcagaacat caaatacaag
    28621 acgagatcag aattgcctta tcaaaacatg gttgcacgat ttttcgatcc aatgcgggac
    28681 aagtgacaac gatcgaaggg cggcaatttt acgggatgcc aaaagggttc ccagatctat
    28741 gtggacatcg taattctgat gggaaagcaa tttatataga agtaaaaaat gaacgcggta
    28801 agttgcgtga agatcaaaaa aggtttgctg aatttttaaa cggtcaacct gtactatacg
    28861 gtgttgcacg atcagtggaa gaagcattga aaattgtgga ggaatcatca tgacaacaga
    28921 agaagtgatt caaatgcgta ttcgaagcct tcagcgtgag attgacgatc tggaacggac
    28981 aaaggcagtg atggtcaatg aaacggcgag gaaggcaatc gatttgcaca tagagaattt
    29041 aagaagggaa atccatcgat tggaggaatg agcgtggata agaaagcagc aatgaaacga
    29101 atcatcgaac tgacacattc tgagaattgg caagaagaca aagaaatagt tgcagaagtc
    29161 caaaagctcg gtaaatcaat gtggagtgaa aaaaccaaac ggagaacacc gagaaaaatt
    29221 gcaatctggc atggtgaccg aattctagta acaggtactg ctgaacagtt atctgaaatt
    29281 actggattaa gcaaaaacat tatctgggat agagctaaac gcgagaacgt tgattcgaaa
    29341 ggtcgtcaat ttaaacattg ggagaagaaa taatggagga actaatcaca aaagtagagc
    29401 agtgggcaaa agataaggga ttggatcaag ctgattccag caagcaaatg ttaaaaacga
    29461 tcgaagagat tggggaagtt gccgcttctc tagctagaaa agatgaatat ggtttaagag
    29521 acggaattgg agacgtagta gtaaccttga ttattttagc tatgcaaaat gatatggatt
    29581 tgtacgagtg tctgaaccaa gcatacaacg aaatcaaagg acgcacagga aaaatggtag
    29641 atggtgtatt cgtgaagtcg agtgatttgg aggacagcga tgaataaaaa agtattaatt
    29701 gataaacaag cattgattga tgaattaatg aaaatacctg gtgtgggatc taatagtgac
    29761 gctttagaaa cgattaaacg tttcccatcg tatgaaccgc agaagccagt tgtgcctaag
    29821 tttgtggcgg aactactcga ctattatcgt cagtcaacag atgttgattt attagcttta
    29881 ttgataactt tccatgattg gtattatcgc aaaactaaag acggtgagca tgaagaagca
    29941 attgattggc tagttgatca tcctgaaatt tatatgcgtg catggctgtt tggctacgag
    30001 ctcgagaaag agccgttgta ttgcgtaata atcaatggta gatatcttgt caaagtattc
    30061 agcaatacaa acgttgtcat attagttcca gcagatgaat tttcacagta cgttactcaa
    30121 aaatttcaat taactgaaaa acaaatcaaa gcaattgatg aaagatactg gccgtttgct
    30181 gtgccagtgg atgaggtggt agaaggatga gcgacccata cattactaaa ttagaaaagt
    30241 tgtccgaaga ttttgagaag aataaaaata aactaactaa agataaactt atagaactgg
    30301 ttaggttggt tatcagagga gctattacaa gagaaaaggc gcagcaaaac atagaaattt
    30361 ttgctcgtag tgtccgagat ggattagaga agaaagttaa acaaaatgaa caacagacac
    30421 cgcagaataa caaaactaag aaaacgggaa ctgaatgtac taaagacaaa gtttgaaaaa
    30481 gaatatggaa tttcagcaga agaaacatat aaagtggcaa gtcagtgtgt tgctgatgcg
    30541 agcggcgcta ttcgtaagtt tggaatttcg atattaagtg atgatcgtaa atgggaggaa
    30601 aaagaatgaa actaaaagac ggattttact ccagcagtca tggtatcggc ggtttaatgc
    30661 tagatatgcc gacaaagaac cctaaaacac gtgagaaacc aaaattcaaa gtcggtgaca
    30721 tggttcgctg tgaagcagaa ggattcatct atccattttg tggatatgta gaacatctct
    30781 ataatcactc agcaatcatt cgtattgaaa acacgatgga atgtgacaag tggttagcga
    30841 aaagcaaaga gaatttagca gtagcgagat tggtggatat ggaggttata taaacagcga
    30901 aaaaatgtac ttctaagaga aaaatttatt ttttataaaa ataggaaagt atcattatga
    30961 aaaagaccac agagataaat aaacctacca cataaaaaat gaatatttct tgcgattcaa
    31021 tataggtata tttaacaatt tgtacaataa aaaatactat aggtaaaaaa gataacatga
    31081 ggagcttatt tgttctagag tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa
    31141 cgggaatcca aaataacagc atgctccaca tagtcactca accatctcct atacgcttta
    31201 ttatcaatag ataatatcat ttttcgtaat aaagataaaa aaataagtaa ggcttctcat
    31261 taaaatataa aaaagacagc cgaccactga ctgcctatat aagagtattg aaataaaaag
    31321 ctgctgatat aataaattcc acaagtttat tatatcacat aaaggagcgg tttgacttga
    31381 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
    31441 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
    31501 ttattacgga tatgccgaag gcgccgaagc acggcaataa ggcagaagac gcaatcattc
    31561 agatgatgga tatagaagcg gagagagacg cgattctagc agccttgatg gctcttagtc
    31621 tgattagtcg tcagatactc tactatagct tttgtgtgcc agatagcttc tcaaactaca
    31681 gaattagccg tgaagtgggt tattcagaaa gaagtataca acggatgaag tcggaagctc
    31741 taatagagtt tgcagaagca tataaacacg gaagaataat tgcttataaa taatttggcg
    31801 gctttttggc ggaatgatgg cggtttttag ctatttacca gtgatattat ggtagtgtcg
    31861 aaagattagt gataggtcta agacaaaata ataataaaag gaacatcgtt ttattattgt
    31921 ttcacaatta agcttcgata gacagcaacg gaaatattaa gaataaggat gtgaa
//
LOCUS       DAZKCE010000021        31452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000021 DAZKCE010000000
VERSION     DAZKCE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.021"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8KQ_000875"
     CDS             212..604
                     /locus_tag="ACG8KQ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9103837.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8KQ_000876"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8KQ_000876"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9103838.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8KQ_000877"
     CDS             2698..2943
                     /locus_tag="ACG8KQ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103839.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8KQ_000878"
     CDS             3165..4775
                     /locus_tag="ACG8KQ_000878"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9103840.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8KQ_000879"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8KQ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103841.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8KQ_000880"
     CDS             5067..5936
                     /locus_tag="ACG8KQ_000880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9103842.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8KQ_000881"
     CDS             6101..7372
                     /locus_tag="ACG8KQ_000881"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9103843.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8KQ_000882"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8KQ_000882"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9103844.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8KQ_000883"
     CDS             8763..9026
                     /locus_tag="ACG8KQ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9103845.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8KQ_000884"
     CDS             9471..9920
                     /locus_tag="ACG8KQ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9103846.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8KQ_000885"
     CDS             9977..11395
                     /locus_tag="ACG8KQ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9103847.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8KQ_000886"
     CDS             11395..12255
                     /locus_tag="ACG8KQ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9103848.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8KQ_000887"
     CDS             12429..13202
                     /locus_tag="ACG8KQ_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9103849.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8KQ_000888"
     CDS             13349..13714
                     /locus_tag="ACG8KQ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9103850.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8KQ_000889"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8KQ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9103851.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8KQ_000890"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8KQ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103852.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8KQ_000891"
     CDS             15560..16216
                     /locus_tag="ACG8KQ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9103853.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8KQ_000892"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8KQ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9103854.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8KQ_000893"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8KQ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9103855.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8KQ_000894"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8KQ_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9103856.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8KQ_000895"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8KQ_000895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9103857.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8KQ_000896"
     CDS             21888..22202
                     /locus_tag="ACG8KQ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9103858.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8KQ_000897"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8KQ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9103859.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8KQ_000898"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8KQ_000898"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9103860.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8KQ_000899"
     CDS             23546..23875
                     /locus_tag="ACG8KQ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9103861.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8KQ_000900"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8KQ_000900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9103862.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8KQ_000901"
     CDS             24863..25687
                     /locus_tag="ACG8KQ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9103863.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8KQ_000902"
     CDS             25680..26027
                     /locus_tag="ACG8KQ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9103864.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8KQ_000903"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8KQ_000903"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9103865.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8KQ_000904"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8KQ_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9103866.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8KQ_000905"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8KQ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9103867.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8KQ_000906"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8KQ_000906"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9103868.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10111 a         5635 c         6633 g         9073 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gc
//
LOCUS       DAZKCE010000022        30168 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000022 DAZKCE010000000
VERSION     DAZKCE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.022"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG8KQ_000907"
     CDS             complement(159..1469)
                     /locus_tag="ACG8KQ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9103869.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2839)
                     /locus_tag="ACG8KQ_000908"
     CDS             complement(1481..2839)
                     /locus_tag="ACG8KQ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9103870.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3041..4609)
                     /locus_tag="ACG8KQ_000909"
     CDS             complement(3041..4609)
                     /locus_tag="ACG8KQ_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9103871.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..5023)
                     /locus_tag="ACG8KQ_000910"
     CDS             complement(4613..5023)
                     /locus_tag="ACG8KQ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9103872.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5020..5943)
                     /locus_tag="ACG8KQ_000911"
     CDS             complement(5020..5943)
                     /locus_tag="ACG8KQ_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9103873.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5940..6818)
                     /locus_tag="ACG8KQ_000912"
     CDS             complement(5940..6818)
                     /locus_tag="ACG8KQ_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9103874.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6805..8142)
                     /locus_tag="ACG8KQ_000913"
     CDS             complement(6805..8142)
                     /locus_tag="ACG8KQ_000913"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9103875.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8145..9248)
                     /locus_tag="ACG8KQ_000914"
     CDS             complement(8145..9248)
                     /locus_tag="ACG8KQ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9103876.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9322..10830)
                     /locus_tag="ACG8KQ_000915"
     CDS             complement(9322..10830)
                     /locus_tag="ACG8KQ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9103877.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10796..11488)
                     /locus_tag="ACG8KQ_000916"
     CDS             complement(10796..11488)
                     /locus_tag="ACG8KQ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9103878.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11510..12484)
                     /locus_tag="ACG8KQ_000917"
     CDS             complement(11510..12484)
                     /locus_tag="ACG8KQ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103879.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12784..13656
                     /locus_tag="ACG8KQ_000918"
     CDS             12784..13656
                     /locus_tag="ACG8KQ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103880.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14114..16312)
                     /locus_tag="ACG8KQ_000919"
     CDS             complement(14114..16312)
                     /locus_tag="ACG8KQ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103881.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16544..17536
                     /locus_tag="ACG8KQ_000920"
     CDS             16544..17536
                     /locus_tag="ACG8KQ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103882.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17596..19017
                     /locus_tag="ACG8KQ_000921"
     CDS             17596..19017
                     /locus_tag="ACG8KQ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9103883.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19010..20509
                     /locus_tag="ACG8KQ_000922"
     CDS             19010..20509
                     /locus_tag="ACG8KQ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9103884.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20548..21942
                     /gene="uxaC"
                     /locus_tag="ACG8KQ_000923"
     CDS             20548..21942
                     /gene="uxaC"
                     /locus_tag="ACG8KQ_000923"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9103885.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22101..22784
                     /locus_tag="ACG8KQ_000924"
     CDS             22101..22784
                     /locus_tag="ACG8KQ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9103886.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22786..23622
                     /locus_tag="ACG8KQ_000925"
     CDS             22786..23622
                     /locus_tag="ACG8KQ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9103887.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23633..25387
                     /locus_tag="ACG8KQ_000926"
     CDS             23633..25387
                     /locus_tag="ACG8KQ_000926"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103888.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25663..26004)
                     /locus_tag="ACG8KQ_000927"
     CDS             complement(25663..26004)
                     /locus_tag="ACG8KQ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9103889.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26072..28243)
                     /locus_tag="ACG8KQ_000928"
     CDS             complement(26072..28243)
                     /locus_tag="ACG8KQ_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9103890.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28454..29311
                     /locus_tag="ACG8KQ_000929"
     CDS             28454..29311
                     /locus_tag="ACG8KQ_000929"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9103891.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29382..>30168)
                     /locus_tag="ACG8KQ_000930"
     CDS             complement(29382..>30168)
                     /locus_tag="ACG8KQ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9103892.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9028 a         6223 c         5346 g         9571 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgcgaaataa aaaaatgcaa
     4681 aaggaaacgc aataaactga gtgccaatca acaatatgaa tcctgttata aaatacaaac
     4741 cagtggcagt tgttaagtat tttcctttag cagtcaaaaa caatgctact gctaaaaaag
     4801 caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat aatccaaatg
     4861 tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt tttttaaact
     4921 ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc aaagatagac
     4981 tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa ccccctgctt
     5041 ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat tacgttcaga
     5101 gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag ttgggtgaac
     5161 atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc acggtcttcc
     5221 taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa aacgataccc
     5281 tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt tactggctaa
     5341 gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga aatccaccgt
     5401 tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg cagaagccgg
     5461 tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc
     5521 ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat tgaataaagc
     5581 aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa gattttctaa
     5641 tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta actggctacc
     5701 gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc ctgctcccac
     5761 aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt cttttactat
     5821 caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt cttggatagt
     5881 ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct
     5941 cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga
     6001 ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg
     6061 tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca
     6121 actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct
     6181 gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa
     6241 agtatttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat
     6301 gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga
     6361 gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc
     6421 attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata
     6481 cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc
     6541 agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc
     6601 ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa cggtccttct
     6661 ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta caattgatag
     6721 tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt
     6781 ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac
     6841 cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga tttctttagt
     6901 attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac
     6961 cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc ctgtcatcat
     7021 agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat
     7081 ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct
     7141 tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata
     7201 agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt
     7261 cagtataaat ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac
     7321 gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat
     7381 acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta atacgacacc
     7441 gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc
     7501 gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca
     7561 attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc
     7621 cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat
     7681 cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg
     7741 gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg
     7801 ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac
     7861 attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg
     7921 aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg
     7981 aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac
     8041 tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt
     8101 caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact
     8161 tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca
     8221 ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga
     8281 cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat
     8341 ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt
     8401 attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct
     8461 ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga
     8521 agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca
     8581 tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa
     8641 aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat
     8701 gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt
     8761 ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa
     8821 ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa
     8881 aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct
     8941 tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt
     9001 acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg
     9061 aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa
     9121 agtaattgga accctttaag tacgacgcca tagtcataag accattttcc tttataccac
     9181 ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag
     9241 ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag
     9301 tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg
     9361 tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc
     9421 gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga
     9481 tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc
     9541 ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga
     9601 gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc
     9661 catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc
     9721 gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt
     9781 cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc
     9841 atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg
     9901 cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac
     9961 cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg
    10021 ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa
    10081 agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc
    10141 ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc
    10201 actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt
    10261 ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc
    10321 tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat
    10381 cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg
    10441 acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa
    10501 cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag
    10561 ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt
    10621 gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg
    10681 tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga
    10741 agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag
    10801 acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat
    10861 ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag
    10921 aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac
    10981 ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg
    11041 agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt
    11101 tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg
    11161 cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta
    11221 aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt
    11281 taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc
    11341 taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga
    11401 tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc
    11461 cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc
    11521 cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga
    11581 cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg
    11641 tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt
    11701 acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga
    11761 ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat
    11821 ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga
    11881 tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt
    11941 caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata
    12001 cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga
    12061 agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat
    12121 ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca
    12181 agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat
    12241 gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag
    12301 caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca
    12361 gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc
    12421 tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt
    12481 ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact
    12541 atctgaatta cgtatataat catttttacc gtaagtaaaa acgcttacat agcaatgatt
    12601 tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc
    12661 ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc
    12721 gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc
    12781 tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt
    12841 ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt
    12901 gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta
    12961 gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt
    13021 ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt
    13081 ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg
    13141 ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat
    13201 tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat cattatgaga
    13261 aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca
    13321 gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc
    13381 tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac
    13441 gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg
    13501 atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct
    13561 gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt
    13621 gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta
    13681 gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta
    13741 aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca
    13801 gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga
    13861 cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa
    13921 aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact
    13981 gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat
    14041 aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa
    14101 acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct
    14161 ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt
    14221 ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa
    14281 caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt
    14341 tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga
    14401 cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc
    14461 attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat
    14521 aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc
    14581 ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac
    14641 tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca
    14701 cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc
    14761 taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg
    14821 gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt
    14881 cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt
    14941 ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat
    15001 ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc
    15061 gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac
    15121 gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt
    15181 cattttgaga actataacca aattgctgca accgctgatc tagttctcgc ctttctactc
    15241 gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt
    15301 gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta
    15361 gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg
    15421 gacgggcaaa tctgtccgaa aataagtagg aaagaagacc aatgagtcct accagtgtta
    15481 aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt
    15541 caacttctaa tctgtaaatc caattattat aatctgattt cagtactata tacgttttat
    15601 ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg
    15661 gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag
    15721 aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag
    15781 caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc
    15841 ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact
    15901 tttcaatttc ctcttcggtc agttgactca acgatccatt gattactccc catttaccag
    15961 ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt
    16021 ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt
    16081 ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc
    16141 attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt
    16201 cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc
    16261 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
    16321 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
    16381 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
    16441 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
    16501 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
    16561 tgtcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
    16621 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
    16681 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
    16741 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
    16801 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
    16861 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
    16921 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
    16981 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
    17041 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
    17101 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
    17161 tgtcaatcca gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
    17221 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
    17281 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
    17341 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
    17401 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
    17461 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
    17521 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
    17581 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
    17641 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
    17701 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
    17761 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
    17821 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
    17881 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
    17941 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
    18001 actcccccac agagcctccc gggcaaactg acagcctttt tataccgacg tttcaaatta
    18061 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
    18121 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
    18181 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
    18241 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
    18301 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
    18361 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
    18421 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
    18481 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
    18541 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
    18601 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
    18661 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
    18721 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
    18781 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
    18841 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
    18901 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
    18961 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
    19021 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
    19081 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
    19141 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
    19201 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
    19261 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
    19321 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
    19381 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
    19441 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
    19501 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
    19561 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
    19621 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
    19681 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
    19741 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
    19801 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
    19861 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
    19921 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
    19981 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
    20041 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
    20101 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
    20161 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
    20221 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
    20281 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
    20341 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
    20401 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
    20461 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
    20521 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
    20581 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
    20641 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
    20701 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
    20761 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
    20821 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
    20881 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
    20941 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
    21001 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
    21061 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
    21121 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
    21181 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
    21241 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
    21301 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
    21361 cgtacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
    21421 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
    21481 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
    21541 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
    21601 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
    21661 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
    21721 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
    21781 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
    21841 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
    21901 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
    21961 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
    22021 agacggacca aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc
    22081 tttatgaaag aagagataac atgcctacta tccatattgc cggtgattct actgcggcag
    22141 aaaaagatga aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt
    22201 cacccgagat ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc
    22261 aggaaggaag gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac
    22321 aatttggaca taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct
    22381 atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct
    22441 tactgacatc tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac
    22501 ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag
    22561 atgtgttctc aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat
    22621 tctacctgca tcttatacct gatacttgta aaaattaccc agaaggacta aaagacaata
    22681 cccactttag tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag
    22741 aatcccgtct gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat
    22801 ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta
    22861 tcacagcttc acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt
    22921 aaattaactg acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac
    22981 aatatcgaac tttccatttt aggatgctat atcaacctat ccagcaaaaa tcaagaagtt
    23041 cgtgcacaag cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc
    23101 aagatgattg ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact
    23161 gaagaagctt atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat
    23221 tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta
    23281 gctaaacgtt taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg
    23341 aatttgatgc ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac
    23401 aatttacatg gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag
    23461 atccgcatgg tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc
    23521 aaatatgagc gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct
    23581 gaagcaatag aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga
    23641 acgaatacag aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct
    23701 ttatcaggga aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct
    23761 tccatcttgg ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga
    23821 ttcttctcag ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca
    23881 aaatatattg acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga
    23941 agctggacta agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa
    24001 agaattgcaa tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac
    24061 gttgtcctct caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt
    24121 aggtatcacg ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct
    24181 cgccgcattc gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag
    24241 gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc
    24301 tcgagctcat gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat
    24361 ctatgaaaaa ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc
    24421 tagttttcag atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg
    24481 gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc
    24541 cgtttctgga atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc
    24601 tattggcgaa ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca
    24661 acatttggat ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa
    24721 accgattatc gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg
    24781 gccatctggc tctggaaaat ctacactcct taacttattg attggattta tccagccgac
    24841 ttcaggaaaa atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg
    24901 aaattattta gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa
    24961 catcacttat ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc
    25021 gcaactttct tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca
    25081 tggcaacaag ctgtctggtg gccaaaagca acgtatctcc attgcacgag cgttgatacg
    25141 tcagccaaaa atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa
    25201 aaaaatccag caagccctga attacctgac agaaacaccc actactttta ttgtggctca
    25261 tcggctttca acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt
    25321 tgaaataggt acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa
    25381 tgaatgacaa aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa
    25441 acggtgttct cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
    25501 tgttttttga aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt
    25561 aaatggtgtt caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc
    25621 cgaacgtctc tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc
    25681 gcgaactggt gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc
    25741 tttttgcatc aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc
    25801 tttcattgca tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat
    25861 ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc
    25921 gtacgcttct ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt
    25981 cgcgctatcg tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat
    26041 tgtaacatgg ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt
    26101 ctttagccgc ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca
    26161 gcccttcttt tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc
    26221 cattagaatt tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat
    26281 aggcatctgc tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg
    26341 atttgaagac tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg
    26401 attcttcgta acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt
    26461 ggtcatttgc ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg
    26521 tataacctgc aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc
    26581 tggtcatttc atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat
    26641 cgacgatcac cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct
    26701 gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt
    26761 ccagtccacc aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt
    26821 cttctgcttt atcatatcct ttttgtaaac cgatctcatg caaaagccat acagtaggca
    26881 aattcaaact ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa
    26941 aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc
    27001 cagcttccag tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac
    27061 gcatctgtgt agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg
    27121 cttgtacacc acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat
    27181 ctgaagcatt tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat
    27241 aatcctgatc taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa
    27301 gaccatactc gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac
    27361 cagaatctga attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg
    27421 cttgttcttg ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt
    27481 tccgatcatt cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca
    27541 ttcctgccaa agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact
    27601 ttctggaagc gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca
    27661 tggtcaagat ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct
    27721 tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag
    27781 tgatcgtact tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac
    27841 gggcaatccc tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga
    27901 ttactgcatc ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg
    27961 cgtacagtgt gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc
    28021 ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca
    28081 ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga
    28141 tgcgctttcg ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga
    28201 tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc
    28261 tatttgattc aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt
    28321 gtagttcaat atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa
    28381 atgtaagttt tttcttaaat atgttttaaa aatggtacaa ttagtctgcg attgcactga
    28441 aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat
    28501 tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat
    28561 acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa
    28621 tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg
    28681 agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc
    28741 tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt
    28801 agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga
    28861 aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat
    28921 gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa
    28981 agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt
    29041 agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga
    29101 acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt
    29161 agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg
    29221 actgatgctc catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt
    29281 cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca
    29341 gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt
    29401 tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata
    29461 tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg
    29521 attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt
    29581 gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa
    29641 acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt
    29701 cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca
    29761 tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt
    29821 tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta
    29881 ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt
    29941 gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc
    30001 cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca
    30061 atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat
    30121 acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaatttt
//
LOCUS       DAZKCE010000023        29100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000023 DAZKCE010000000
VERSION     DAZKCE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.023"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..829
                     /locus_tag="ACG8KQ_000931"
     CDS             <1..829
                     /locus_tag="ACG8KQ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9103893.1"
                     /translation="VETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLT
                     KIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGY
                     ELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGT
                     VLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTK
                     ENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1182..2030)
                     /locus_tag="ACG8KQ_000932"
                     /pseudo
     CDS             complement(1182..2030)
                     /locus_tag="ACG8KQ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2183..3295
                     /locus_tag="ACG8KQ_000933"
     CDS             2183..3295
                     /locus_tag="ACG8KQ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9103894.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3373..3666)
                     /locus_tag="ACG8KQ_000934"
     CDS             complement(3373..3666)
                     /locus_tag="ACG8KQ_000934"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9103895.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3834..4148
                     /locus_tag="ACG8KQ_000935"
     CDS             3834..4148
                     /locus_tag="ACG8KQ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9103896.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4169..6148
                     /locus_tag="ACG8KQ_000936"
     CDS             4169..6148
                     /locus_tag="ACG8KQ_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9103897.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6161..6487
                     /locus_tag="ACG8KQ_000937"
     CDS             6161..6487
                     /locus_tag="ACG8KQ_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9103898.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6503..7021
                     /locus_tag="ACG8KQ_000938"
     CDS             6503..7021
                     /locus_tag="ACG8KQ_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103899.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7021..8373
                     /gene="celB"
                     /locus_tag="ACG8KQ_000939"
     CDS             7021..8373
                     /gene="celB"
                     /locus_tag="ACG8KQ_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9103900.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8457..9962)
                     /locus_tag="ACG8KQ_000940"
     CDS             complement(8457..9962)
                     /locus_tag="ACG8KQ_000940"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9103901.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10054..10644)
                     /locus_tag="ACG8KQ_000941"
     CDS             complement(10054..10644)
                     /locus_tag="ACG8KQ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9103902.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10823..12469
                     /locus_tag="ACG8KQ_000942"
     CDS             10823..12469
                     /locus_tag="ACG8KQ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9103903.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12518..13237
                     /locus_tag="ACG8KQ_000943"
     CDS             12518..13237
                     /locus_tag="ACG8KQ_000943"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9103904.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13716..14663
                     /locus_tag="ACG8KQ_000944"
     CDS             13716..14663
                     /locus_tag="ACG8KQ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103905.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14675..15595
                     /locus_tag="ACG8KQ_000945"
     CDS             14675..15595
                     /locus_tag="ACG8KQ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103906.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15636..17093
                     /locus_tag="ACG8KQ_000946"
     CDS             15636..17093
                     /locus_tag="ACG8KQ_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9103907.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17438..18052
                     /locus_tag="ACG8KQ_000947"
     CDS             17438..18052
                     /locus_tag="ACG8KQ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9103908.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18045..19769
                     /locus_tag="ACG8KQ_000948"
     CDS             18045..19769
                     /locus_tag="ACG8KQ_000948"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9103909.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19766..21217
                     /locus_tag="ACG8KQ_000949"
     CDS             19766..21217
                     /locus_tag="ACG8KQ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9103910.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21690..23843)
                     /locus_tag="ACG8KQ_000950"
     CDS             complement(21690..23843)
                     /locus_tag="ACG8KQ_000950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9103911.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23845..24891)
                     /locus_tag="ACG8KQ_000951"
     CDS             complement(23845..24891)
                     /locus_tag="ACG8KQ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9103912.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25041..26330
                     /locus_tag="ACG8KQ_000952"
     CDS             25041..26330
                     /locus_tag="ACG8KQ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9103913.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26343..29045
                     /locus_tag="ACG8KQ_000953"
     CDS             26343..29045
                     /locus_tag="ACG8KQ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9103914.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9462 a         5031 c         5903 g         8704 t
ORIGIN      
        1 ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg agtttgagat
       61 cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta caccaggcgg
      121 tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag ctgtttttga
      181 gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag cagatggaaa
      241 actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc aagcgccaac
      301 ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc agacagcggt
      361 ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga caaagatcga
      421 tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      481 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag atggcttagc
      541 accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat tggatgcgac
      601 accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa caaaagagaa
      661 tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca gtggaaataa
      721 gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa tattgggtgt
      781 gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag ctaaaataaa
      841 aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc
      901 gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt tgaacccaac
      961 cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa acctttgtag
     1021 taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg agtttcgcag
     1081 tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt tctacaaata
     1141 atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa taaggagaaa
     1201 aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa ggttacgcct
     1261 ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca tttggtagaa
     1321 catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat tctatttctt
     1381 taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt gtaggtaaat
     1441 tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt tatcctcaac
     1501 ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct tttggaagac
     1561 tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa agatacctaa
     1621 caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt gattttatgt
     1681 ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc gttccaatgg
     1741 cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat tggcatatga
     1801 ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac cattaatttc
     1861 ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc agggaagaga
     1921 actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc
     1981 atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat aaagcatatc
     2041 gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat atgttttttt
     2101 ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata tgatgttcca
     2161 tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga aaggacgaca
     2221 acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac aaaaaatacg
     2281 agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt tacgatcatt
     2341 attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat ttgaatcgaa
     2401 gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt ttgttgactt
     2461 tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct cactattagt
     2521 agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc attttatttt
     2581 actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg cttctttgta
     2641 cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga acaagaattt
     2701 ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag ttctccttta
     2761 cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt ttttgaaagg
     2821 catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta gtcttttgat
     2881 cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg cattttcttc
     2941 tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat
     3001 cattggctca ttgattggat gtgttttatt tattggtatc agccaattta ttccttttaa
     3061 ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata gatataaaac
     3121 agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg ttccaggagc
     3181 agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca tgtatattgg
     3241 ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc attagatgag
     3301 atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg ctatcattcc
     3361 atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta atttatactt
     3421 tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt cgattgcttc
     3481 ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg gaaagtaata
     3541 gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc tttcgaccat
     3601 ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat tcactgtgat
     3661 actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg ccagaaatta
     3721 ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata ttttgaatag
     3781 aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga tttatgaaaa
     3841 aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca aagacaacgg
     3901 atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct acagaaggcg
     3961 ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa acgacgatgt
     4021 ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt agtatccctt
     4081 tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg atcgaagaga
     4141 acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc tgttgagata
     4201 tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg aattgttatt
     4261 atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag aaaaaaacgg
     4321 aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac aaagactgac
     4381 atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag aaccgcaagg
     4441 attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt tggaagatgc
     4501 cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca gtttgacaaa
     4561 ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg tttctaagca
     4621 tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta tcatggatac
     4681 tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa gccgtttttt
     4741 cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa gagaagctaa
     4801 gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt tggggatcaa
     4861 acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag tttctaagcg
     4921 agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc
     4981 gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt cgaatcacaa
     5041 agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga aaagattgtc
     5101 tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac tactcgatca
     5161 tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc cattgactga
     5221 ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat
     5281 gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg ctttgcatct
     5341 catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga ttatctgtgc
     5401 aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa
     5461 tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt taaaaggaat
     5521 cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc cagttttaca
     5581 tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc
     5641 tataccctat cattcctcag atagaccgat accagcattt tctctacagc aaaaaaagca
     5701 gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa
     5761 ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag aaggatatat
     5821 aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca tattcagtga
     5881 tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg tagccgttgc
     5941 attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg tttttttagt
     6001 ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt
     6061 agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg agaatttcag
     6121 cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa aaataaacag
     6181 tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg cacggacact
     6241 gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag aagagaagtt
     6301 gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac ttctgaaaga
     6361 atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac aagatcatct
     6421 gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt atcaaaaaaa
     6481 taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa
     6541 aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac gttacctgac
     6601 tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag ctaaaagttt
     6661 aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag gagaacaagt
     6721 aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt ttttgttcca
     6781 gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta cattcttgta
     6841 tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg ttgtaagcgg
     6901 tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca accaagacca
     6961 acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg agaataacta
     7021 atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc gcaacttaaa
     7081 atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat tgtaggctcg
     7141 atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg attttttaat
     7201 gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac gatggggatt
     7261 ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga acgagatgaa
     7321 caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc attctttatg
     7381 tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc tatcaatgag
     7441 acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt aggaacttca
     7501 ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg tatgtgtgtc
     7561 aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc tcgttctttc
     7621 actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa tggattatta
     7681 gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt tgttacgcag
     7741 ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc attgtggatc
     7801 gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt attagctaat
     7861 atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa caattcttat
     7921 gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc attcctcgcg
     7981 aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt attcaatatc
     8041 aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc ggttccgttt
     8101 ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct ggaaatcgtc
     8161 aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc atttgttggt
     8221 agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat cgcattcatt
     8281 atctggttcc catttatcaa attctatgat aataaattat taaatgaaga aagagaaaag
     8341 gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga actgtgacaa
     8401 aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa ataccattat
     8461 tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc tacaccattg
     8521 attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg cggagcgctt
     8581 tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac gatcaaatct
     8641 ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc aaatgctggg
     8701 tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc aaaatcatgt
     8761 cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg gccatctgga
     8821 tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg tgcatagtcc
     8881 acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg tacacgagct
     8941 tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc ggcaatgatc
     9001 gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata agcagtatcg
     9061 cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc tttatctgaa
     9121 cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg aatctggaat
     9181 agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa aacaactgtt
     9241 tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt cggatgttcg
     9301 atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa agtcaagccg
     9361 tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg agtcatacca
     9421 ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt tgtcaaatgg
     9481 gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa tgctgttttt
     9541 ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt tttacctttt
     9601 gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa agccatagca
     9661 agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga attttcgtat
     9721 ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa atattcttct
     9781 ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct tgaatcataa
     9841 cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct tgttgaaaca
     9901 aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa agagaagctc
     9961 attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact ctttttaata
    10021 tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg acacagggga
    10081 caagcggatg agtattttca taaacaatga actgactgta atagatagga gaacgaatag
    10141 tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat tggtaagtaa
    10201 aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg gtatcgttat
    10261 accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga acaaaagact
    10321 tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa caatctctaa
    10381 aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt ttcctaatag
    10441 gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg agcgttggat
    10501 agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag ctatatcgtg
    10561 agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa atataacggg
    10621 tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat aaaaggctgt
    10681 atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt gttcttataa
    10741 aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata gtaatgtttt
    10801 gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag atcaattaac
    10861 accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata tcatttcaag
    10921 attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa atgctaaggc
    10981 tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac tgatctctac
    11041 agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat tgaatgaata
    11101 tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt taggcctgtt
    11161 gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg gtggcggaga
    11221 agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc cttgtatgag
    11281 tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat
    11341 tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca tcatgaaagt
    11401 catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat ttatcggtat
    11461 gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt atacatcggc
    11521 gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt tgttagatac
    11581 cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct tcaaattagt
    11641 cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca atgcacagtt
    11701 actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg tagttattgc
    11761 attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg ttacgttgaa
    11821 agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt cattttttat
    11881 gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat tattttatac
    11941 accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac tcttcctagg
    12001 actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg cggcaattaa
    12061 ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca ttcgtttatt
    12121 tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt gtaccttgat
    12181 cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc gcaggacatt
    12241 gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa agatgatttt
    12301 tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc ttgtagtagc
    12361 aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg cagaaagact
    12421 actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag tgaatcagaa
    12481 gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata agtttttagc
    12541 agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag gaacgatcga
    12601 agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg aagatgcggt
    12661 tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt tgcataaacc
    12721 tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg atttattgga
    12781 agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag acacagaggg
    12841 cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac ctaaaaaaca
    12901 tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag atatccgttt
    12961 atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa aactccaaat
    13021 tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc atgaaggaaa
    13081 gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag
    13141 aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat atcgacccat
    13201 gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc taaaagaata
    13261 agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt tcggatggct
    13321 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tttgttcgga
    13381 ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga ctcagagtta
    13441 gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca caacctctga
    13501 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
    13561 actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca caacctctcc
    13621 tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg aaagcgctat
    13681 aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc
    13741 atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta ccgggtatgc
    13801 tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt
    13861 ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg gtgggttggg
    13921 aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa aatacaattt
    13981 tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt gcaatcatta
    14041 tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc ttactacctt
    14101 attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt cctgacaaag
    14161 gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg tataatgaac
    14221 cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt ggttataaca
    14281 gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa gctgctatgg
    14341 ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa cttattccac
    14401 taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat tttggcctgt
    14461 tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt ttggatacgt
    14521 atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct gcaggacttt
    14581 accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg
    14641 atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt
    14701 ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga ttgcactctt
    14761 tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta ccgatgagtc
    14821 catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat ttggttatac
    14881 gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca ctgtacttgt
    14941 tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt atgcgatttc
    15001 acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta cgatgttgtt
    15061 ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt taaaagatac
    15121 gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg tgatgcgcac
    15181 attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg atggagctag
    15241 cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta ttgcgacaat
    15301 cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac tatacatcca
    15361 agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta atattcaata
    15421 tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct cagttccagg
    15481 agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga tcagctatcc
    15541 atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa
    15601 caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag aaactagcct
    15661 ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt tctggttcta
    15721 aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc ggagataaac
    15781 cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa gagatcggtg
    15841 taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg aacgtcatca
    15901 tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa
    15961 aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa gcttataatg
    16021 atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat gctttcccag
    16081 tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta ctagataaat
    16141 acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc ttgaaggaat
    16201 tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc aaagtccaag
    16261 ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg gatggagatg
    16321 attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta cgtacaatgc
    16381 atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat aaggattatc
    16441 cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat gattatggcg
    16501 atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt actgttccac
    16561 taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact tcaaaaaata
    16621 aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta ttgaatgggc
    16681 ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat gataaaatca
    16741 aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca ggaaacaaca
    16801 agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt gatcaatcga
    16861 ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac agtgtcaaaa
    16921 cagaattaag taatatttct aatgtcatga atcaatattt agatggattg aatacaggga
    16981 ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag gctggctatg
    17041 ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa taataatgaa
    17101 taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga ataaacggtg
    17161 ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga aagccatatt
    17221 cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct tggtaaacgg
    17281 tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga ggaactattt
    17341 ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt tttttgtata
    17401 atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg ttttggaaga
    17461 gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga tatatacatt
    17521 tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg aactatttta
    17581 tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata tatataaaag
    17641 aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg ctttattgag
    17701 ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta ttgattttat
    17761 tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga ttttagatag
    17821 tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg ttttttccag
    17881 cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg tgacttggca
    17941 attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga atggtacgat
    18001 gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat aagtttcgga
    18061 ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg gttatcatga
    18121 tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa caaaatcaaa
    18181 aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg ctgaatgata
    18241 aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa
    18301 atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta tacgaacagc
    18361 aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc tacgcaaatt
    18421 atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat tatgaatctt
    18481 ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta gacgcctttt
    18541 atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt attggttttc
    18601 aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga ctctctttat
    18661 acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa atagctaaag
    18721 aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat tcacaattgc
    18781 cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct gatgattatc
    18841 gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc
    18901 cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt tataaaacat
    18961 tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa acagcagcag
    19021 ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag gaactttcaa
    19081 tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc tacaaattag
    19141 aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat ccacactttt
    19201 tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt gaagaattag
    19261 cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga
    19321 tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt
    19381 atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag atcgaagtac
    19441 ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt gattttactc
    19501 gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta aggatcatca
    19561 tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa gaaaaactgt
    19621 ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat gaacgtttac
    19681 gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca ggtggactga
    19741 ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg tagatgatga
    19801 atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag gctttcaaat
    19861 agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc gacagattga
    19921 tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta ttgaagcggc
    19981 tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag aattcgatta
    20041 tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg tgaataagtt
    20101 cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa atgaacaaaa
    20161 aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg aagaattgga
    20221 tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata ggatagtatt
    20281 gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag aacggcaaca
    20341 aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt tgttggaggc
    20401 agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc aggaatggct
    20461 gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat tccaacaagc
    20521 gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg ttttttacgc
    20581 agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac gcgtgctgca
    20641 aaataaacaa ttagaagtcg ctcaaaaaat gatcactgat ttttttgaac agtttcagtt
    20701 agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta tggagattca
    20761 acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa aaatccatca
    20821 ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa agcatcagag
    20881 acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc agcattatca
    20941 agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca tgtatctagg
    21001 acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc atttacgtat
    21061 ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga tagcaagtga
    21121 gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg tcggcagatc
    21181 ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa tgtaatgaaa
    21241 taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac ttgaggcaag
    21301 gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc ggtattaagc
    21361 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat actcgaaact
    21421 aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
    21481 gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta atactcaggt
    21541 cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca tctgcggtaa
    21601 taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc ttattacttg
    21661 atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga aaaaggcaaa
    21721 tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga gatcgtgccg
    21781 cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctttgtc catccgtttg
    21841 attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt caattccttt
    21901 ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac atattcaccg
    21961 gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc ggccatactt
    22021 ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg agtcatcaac
    22081 tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa gtaaggatag
    22141 tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc catctcactc
    22201 atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg attgcacagc
    22261 tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag tcctgaaaaa
    22321 tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc ttgtccccaa
    22381 cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg cataaatcct
    22441 gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt cttcgtcatt
    22501 tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata agcatagtct
    22561 aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt caagtaatct
    22621 tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc tttttccatt
    22681 gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc atctgcaatg
    22741 accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag ccattttggc
    22801 aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc gtatttttct
    22861 acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa cccattattt
    22921 gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat tttttgccca
    22981 ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa attatgataa
    23041 aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa ataggactgc
    23101 cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc tagttccagg
    23161 ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt ttgttttttt
    23221 gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc taactcatca
    23281 attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg atcttctgaa
    23341 ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa gagctgatgg
    23401 cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat agataagatt
    23461 ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg acagatcgac
    23521 agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc atggaaaagg
    23581 gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa atcacccatc
    23641 caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc aggatgaaac
    23701 caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc aaaaggaacg
    23761 ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt tgcatgtcta
    23821 gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt tgatgaatga
    23881 aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga ccaaagcatc
    23941 tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg gcgggtcaac
    24001 aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag ggatatgata
    24061 accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt cacaaacaaa
    24121 agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg cgcttttgtc
    24181 atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt aaccaaggta
    24241 acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga tttttgaatg
    24301 tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat cggaaagaac
    24361 ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga attctttcaa
    24421 tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat aaatcaatcc
    24481 gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa gcgtcgtcat
    24541 catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa aatcaccaac
    24601 agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg taggagaagc
    24661 aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa cgcgataaat
    24721 aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc ttactttata
    24781 ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt caccagttaa
    24841 aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgattca tataaacacc
    24901 tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa ataaaaagat
    24961 attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa gatatatcaa
    25021 atagaaagaa ggttttatta gtggcttata aagatatacc aaaatcggtg cagaaattta
    25081 tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag aattttacag
    25141 cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga accacctttt
    25201 tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa gttcgtcctt
    25261 atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg ttagtcaaaa
    25321 agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa gaagctaacg
    25381 gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa agaaaatatg
    25441 aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa aatacaggaa
    25501 aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg gacgtgttca
    25561 aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg acaagaaaag
    25621 aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga atgacatggt
    25681 ctggtttccg tcctagcgat gatacttgcc gttatggata tctggtacca tccaatatgt
    25741 ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc gatgataaag
    25801 aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt cagaatcacg
    25861 gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat ggaatgggaa
    25921 acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct tacttgggat
    25981 attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg agtaaagaaa
    26041 atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat acgccagaaa
    26101 aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac aaatctgaaa
    26161 aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt atgcacgaag
    26221 gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg gcaaatatga
    26281 tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag gaggtatcgt
    26341 aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa tggtacatgg
    26401 cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta gagctatttg
    26461 aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt ttagatgact
    26521 atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat gaaggaaaat
    26581 tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg gaatcgaacg
    26641 tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct gtcatgttag
    26701 gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg aaacaagctg
    26761 gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat aatcaagttt
    26821 tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaagga cctgatcaaa
    26881 cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag attccagtag
    26941 aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa caatatgcct
    27001 ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa aaagatatat
    27061 caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt cattctaatt
    27121 ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc tcagtagaag
    27181 gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca gcatctgctc
    27241 gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat attacagaac
    27301 cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa ttagattatg
    27361 cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt tcagtcgatt
    27421 ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga aagtatcttg
    27481 cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc ccaaaagaca
    27541 gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa gcagaaataa
    27601 ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta tatcaagagt
    27661 tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc actataccag
    27721 ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac cgtttccgtg
    27781 tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg ccagcatttt
    27841 cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag gaaacaatga
    27901 ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt gaaaaaaatg
    27961 gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg catatttttg
    28021 aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt gatcagccaa
    28081 tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca ccggaaatag
    28141 caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag cgtttagaaa
    28201 aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc aaagaaaaac
    28261 gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa atcgactttg
    28321 aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt cctacaaagt
    28381 tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc ccaaaccatg
    28441 tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt gtcaatattc
    28501 atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat gaagtaacag
    28561 aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc gattggggat
    28621 attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac agcattgaac
    28681 tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca cagattcctt
    28741 tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa caatatttaa
    28801 cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt agtgataaag
    28861 tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata actaaagaca
    28921 atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg gttgtcccaa
    28981 taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa tgtatggcgg
    29041 aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag aaattttaga
//
LOCUS       DAZKCE010000024        28965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000024 DAZKCE010000000
VERSION     DAZKCE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.024"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..966)
                     /locus_tag="ACG8KQ_000954"
     CDS             complement(22..966)
                     /locus_tag="ACG8KQ_000954"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9103915.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1262..2206)
                     /locus_tag="ACG8KQ_000955"
     CDS             complement(1262..2206)
                     /locus_tag="ACG8KQ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9103916.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2426..3640
                     /locus_tag="ACG8KQ_000956"
     CDS             2426..3640
                     /locus_tag="ACG8KQ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9103917.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3662..5677
                     /locus_tag="ACG8KQ_000957"
     CDS             3662..5677
                     /locus_tag="ACG8KQ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP9103918.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5760..7133)
                     /locus_tag="ACG8KQ_000958"
     CDS             complement(5760..7133)
                     /locus_tag="ACG8KQ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9103919.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7159..7443)
                     /locus_tag="ACG8KQ_000959"
     CDS             complement(7159..7443)
                     /locus_tag="ACG8KQ_000959"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9103920.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7445..9460)
                     /locus_tag="ACG8KQ_000960"
     CDS             complement(7445..9460)
                     /locus_tag="ACG8KQ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9103921.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9639..9944)
                     /locus_tag="ACG8KQ_000961"
     CDS             complement(9639..9944)
                     /locus_tag="ACG8KQ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9103922.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10033..11063)
                     /locus_tag="ACG8KQ_000962"
                     /pseudo
     CDS             complement(10033..11063)
                     /locus_tag="ACG8KQ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11270..12145
                     /locus_tag="ACG8KQ_000963"
     CDS             11270..12145
                     /locus_tag="ACG8KQ_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9103923.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="ACG8KQ_000964"
     CDS             complement(12297..13139)
                     /locus_tag="ACG8KQ_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9103924.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="ACG8KQ_000965"
     CDS             complement(13231..15108)
                     /locus_tag="ACG8KQ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9103925.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="ACG8KQ_000966"
     CDS             complement(15105..16526)
                     /locus_tag="ACG8KQ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9103926.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="ACG8KQ_000967"
     CDS             complement(16529..19918)
                     /locus_tag="ACG8KQ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9103927.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="ACG8KQ_000968"
     CDS             20175..21566
                     /locus_tag="ACG8KQ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9103928.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21695..22969)
                     /locus_tag="ACG8KQ_000969"
     CDS             complement(21695..22969)
                     /locus_tag="ACG8KQ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFP9103929.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(23148..23795)
                     /locus_tag="ACG8KQ_000970"
     CDS             complement(23148..23795)
                     /locus_tag="ACG8KQ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9103930.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(23803..24489)
                     /locus_tag="ACG8KQ_000971"
     CDS             complement(23803..24489)
                     /locus_tag="ACG8KQ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103931.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24495..25568)
                     /locus_tag="ACG8KQ_000972"
     CDS             complement(24495..25568)
                     /locus_tag="ACG8KQ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9103932.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            25790..26464
                     /locus_tag="ACG8KQ_000973"
     CDS             25790..26464
                     /locus_tag="ACG8KQ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9103933.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26469..27809
                     /locus_tag="ACG8KQ_000974"
     CDS             26469..27809
                     /locus_tag="ACG8KQ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9103934.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27958..28944)
                     /locus_tag="ACG8KQ_000975"
     CDS             complement(27958..28944)
                     /locus_tag="ACG8KQ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9103935.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8425 a         5495 c         5308 g         9737 t
ORIGIN      
        1 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
       61 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
      121 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
      181 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
      241 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
      301 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
      361 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
      421 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
      481 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
      541 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
      601 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
      661 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
      721 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
      781 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
      841 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
      901 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
      961 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
     1021 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
     1081 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
     1141 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
     1201 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
     1261 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
     1321 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
     1381 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
     1441 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
     1501 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
     1561 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
     1621 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
     1681 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
     1741 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
     1801 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
     1861 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
     1921 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
     1981 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
     2041 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
     2101 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2161 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa atgtctaatt
     2221 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     2281 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     2341 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     2401 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     2461 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     2521 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     2581 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     2641 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     2701 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     2761 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     2821 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     2881 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     2941 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     3001 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     3061 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     3121 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     3181 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     3241 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     3301 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     3361 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     3421 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     3481 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     3541 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     3601 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     3661 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     3721 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     3781 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     3841 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     3901 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     3961 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     4021 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     4081 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     4141 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     4201 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     4261 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     4321 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     4381 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     4441 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     4501 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     4561 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     4621 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     4681 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     4741 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     4801 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     4861 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     4921 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     4981 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     5041 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     5101 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     5161 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     5221 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     5281 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     5341 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     5401 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     5461 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     5521 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     5581 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     5641 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     5701 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     5761 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     5821 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     5881 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     5941 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     6001 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     6061 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     6121 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     6181 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     6241 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     6301 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     6361 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     6421 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     6481 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     6541 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     6601 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     6661 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     6721 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     6781 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     6841 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     6901 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     6961 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     7021 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     7081 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     7141 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     7201 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     7261 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     7321 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     7381 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     7441 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     7501 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     7561 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     7621 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     7681 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     7741 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     7801 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     7861 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     7921 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     7981 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     8041 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     8101 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     8161 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     8221 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     8281 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     8341 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     8401 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     8461 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     8521 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     8581 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     8641 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     8701 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     8761 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     8821 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     8881 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     8941 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     9001 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     9061 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     9121 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     9181 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     9241 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     9301 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     9361 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     9421 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     9481 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     9541 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     9601 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     9661 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     9721 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     9781 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     9841 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
     9901 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
     9961 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    10021 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    10081 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    10141 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    10201 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    10261 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    10321 ttttgtttgt ttcacgaaca tccctttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaatgg ctcttcgtca aattgtgttg gcgaagacaa atttgaataa aacttagtcc
    21661 gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc agaccttgtt
    21721 gtaaatagat ccgtgcccga aagttataaa agttgcgata accaaacgca attcgtttaa
    21781 tcagcttgat cttgttattc agcccctcag tgacgccgtt tgagtggggc accttaaagg
    21841 catttgaaat acccgattgg tagcgatgga agatcgtgaa tttcttttta aacgactgag
    21901 gtaacgtaga tgaaattgtt ttaagggttt cttgaaagcc ttccgtatct cgtttaacga
    21961 aataatagcg taataattga caaaaatgat acgcagttcg caattcttcg ttgtagctta
    22021 acagctcatc aacgatttct gtttgactaa ttggtcgttt aaacagtcga tgataatgcc
    22081 gtttgagtgg ctccaatgtc gtggaatctt tgagtaagag tttccaatat cgcttgagcc
    22141 gacggtactt cttctgattt tcagagtgtg aggttcgaaa acgattcatg atctggacac
    22201 gtaattgatt gaagctgcga ttgatgtgtt ggacaatatg aaagcggtca gttacgatct
    22261 ccgcatttgg aaagactgtt ttaagcaact gacaatagct ggcattcatg tccatcacta
    22321 ggtacttgac ctttaaacgg gcctttctag gaaagcgcag gaagtattga gttagtttgt
    22381 aaagtctgcg atcttcaagg atcgtaaata gctgattggt tgtcccatca acacaaataa
    22441 aactcatgga accagaaaca gacttcattg atttgaactc atccatacac aacaccgtcg
    22501 gaaggaagtt catacgtggc tggtaatgct tcgttgcttc acgcaagatc cgtgtcaccg
    22561 tcccatccga gacgaagtac ttcttagcga ttagtttacg tgattgattt tcgtacagtt
    22621 ccatgagaat cgcctgcttc agttcctttg ttagttgatg atgttcatca actaagtccg
    22681 ttttcgcaga aaaggtccgt ccacaatcat ggcagagaaa acgggtacgt tttaattcta
    22741 aaatagtcaa cttttctttg actttcaaca tctgagtgcg tgtcgtatag gaaccgttgc
    22801 ggataatacg aatcgatcca cagtgttcac aatgggaagg tgagtcatcc aatctccctt
    22861 taataaactg cgcttgaacg cctttatatt ctttttcaat cagccattct tcatcaaaaa
    22921 ataaatgttt atctgttaat ccgagtaatt ttctagtatg attatccatt gagagactcc
    22981 tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt
    23041 gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat
    23101 tatagaacca taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    23161 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    23221 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    23281 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    23341 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    23401 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    23461 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    23521 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    23581 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    23641 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    23701 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    23761 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    23821 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    23881 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    23941 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    24001 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    24061 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    24121 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    24181 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    24241 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    24301 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    24361 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    24421 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    24481 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    24541 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    24601 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    24661 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    24721 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    24781 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    24841 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    24901 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    24961 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    25021 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    25081 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    25141 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    25201 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    25261 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    25321 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    25381 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    25441 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    25501 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    25561 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    25621 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    25681 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    25741 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    25801 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    25861 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    25921 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    25981 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    26041 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    26101 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    26161 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    26221 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    26281 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    26341 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    26401 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    26461 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    26521 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    26581 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    26641 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    26701 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    26761 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    26821 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    26881 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    26941 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    27001 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    27061 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    27121 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    27181 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    27241 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    27301 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    27361 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    27421 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    27481 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    27541 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    27601 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    27661 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    27721 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    27781 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    27841 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    27901 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    27961 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    28021 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    28081 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    28141 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    28201 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    28261 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    28321 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    28381 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    28441 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    28501 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    28561 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    28621 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    28681 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    28741 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    28801 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    28861 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    28921 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaata
//
LOCUS       DAZKCE010000025        28637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000025 DAZKCE010000000
VERSION     DAZKCE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.025"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8KQ_000976"
     CDS             85..624
                     /locus_tag="ACG8KQ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103936.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACG8KQ_000977"
     CDS             1644..2813
                     /locus_tag="ACG8KQ_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9103937.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="ACG8KQ_000978"
     CDS             3286..3840
                     /locus_tag="ACG8KQ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9103938.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4450)
                     /locus_tag="ACG8KQ_000979"
     CDS             complement(4013..4450)
                     /locus_tag="ACG8KQ_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9103939.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5445
                     /locus_tag="ACG8KQ_000980"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="ACG8KQ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6151
                     /locus_tag="ACG8KQ_000981"
                     /pseudo
     CDS             5716..6151
                     /locus_tag="ACG8KQ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6167..7000
                     /locus_tag="ACG8KQ_000982"
     CDS             6167..7000
                     /locus_tag="ACG8KQ_000982"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103940.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="ACG8KQ_000983"
     CDS             7245..8270
                     /locus_tag="ACG8KQ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103941.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="ACG8KQ_000984"
     CDS             8408..8533
                     /locus_tag="ACG8KQ_000984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103942.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="ACG8KQ_000985"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="ACG8KQ_000985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="ACG8KQ_000986"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="ACG8KQ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="ACG8KQ_000987"
     CDS             10668..11492
                     /locus_tag="ACG8KQ_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103943.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="ACG8KQ_000988"
     CDS             11524..12834
                     /locus_tag="ACG8KQ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFP9103944.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="ACG8KQ_000989"
     CDS             12982..14241
                     /locus_tag="ACG8KQ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9103945.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="ACG8KQ_000990"
     CDS             14251..15123
                     /locus_tag="ACG8KQ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103946.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="ACG8KQ_000991"
     CDS             15135..15965
                     /locus_tag="ACG8KQ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103947.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="ACG8KQ_000992"
     CDS             16005..18188
                     /locus_tag="ACG8KQ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9103948.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="ACG8KQ_000993"
     CDS             18249..19469
                     /locus_tag="ACG8KQ_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9103949.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8KQ_000994"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8KQ_000994"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFP9103950.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="ACG8KQ_000995"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="ACG8KQ_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="ACG8KQ_000996"
     CDS             22431..22640
                     /locus_tag="ACG8KQ_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9103951.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="ACG8KQ_000997"
     CDS             22633..22782
                     /locus_tag="ACG8KQ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9103952.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="ACG8KQ_000998"
     CDS             22936..23601
                     /locus_tag="ACG8KQ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9103953.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8KQ_000999"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8KQ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9103954.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="ACG8KQ_001000"
     CDS             25304..25537
                     /locus_tag="ACG8KQ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFP9103955.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="ACG8KQ_001001"
     CDS             25534..25941
                     /locus_tag="ACG8KQ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9103956.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="ACG8KQ_001002"
     CDS             complement(27078..27596)
                     /locus_tag="ACG8KQ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP9103957.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="ACG8KQ_001003"
     CDS             27963..28211
                     /locus_tag="ACG8KQ_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103958.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9843 a         4314 c         5245 g         9235 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagc
//
LOCUS       DAZKCE010000026        28441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000026 DAZKCE010000000
VERSION     DAZKCE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.026"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..166
                     /locus_tag="ACG8KQ_001004"
     CDS             <1..166
                     /locus_tag="ACG8KQ_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9103959.1"
                     /translation="KKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENR
                     KKAMRKRVSR"
     gene            complement(228..2039)
                     /locus_tag="ACG8KQ_001005"
     CDS             complement(228..2039)
                     /locus_tag="ACG8KQ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP9103960.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2036..2401)
                     /locus_tag="ACG8KQ_001006"
     CDS             complement(2036..2401)
                     /locus_tag="ACG8KQ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9103961.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2564..2959)
                     /locus_tag="ACG8KQ_001007"
     CDS             complement(2564..2959)
                     /locus_tag="ACG8KQ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9103962.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2977..3939)
                     /locus_tag="ACG8KQ_001008"
     CDS             complement(2977..3939)
                     /locus_tag="ACG8KQ_001008"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9103963.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3939..4151)
                     /locus_tag="ACG8KQ_001009"
     CDS             complement(3939..4151)
                     /locus_tag="ACG8KQ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9103964.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4172..4870)
                     /locus_tag="ACG8KQ_001010"
     CDS             complement(4172..4870)
                     /locus_tag="ACG8KQ_001010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9103965.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4890..5432)
                     /locus_tag="ACG8KQ_001011"
     CDS             complement(4890..5432)
                     /locus_tag="ACG8KQ_001011"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9103966.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5564..6568)
                     /locus_tag="ACG8KQ_001012"
     CDS             complement(5564..6568)
                     /locus_tag="ACG8KQ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9103967.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6565..7554)
                     /locus_tag="ACG8KQ_001013"
     CDS             complement(6565..7554)
                     /locus_tag="ACG8KQ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9103968.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7551..8357)
                     /locus_tag="ACG8KQ_001014"
     CDS             complement(7551..8357)
                     /locus_tag="ACG8KQ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9103969.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8523..9479
                     /locus_tag="ACG8KQ_001015"
     CDS             8523..9479
                     /locus_tag="ACG8KQ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9103970.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9556..10074)
                     /locus_tag="ACG8KQ_001016"
     CDS             complement(9556..10074)
                     /locus_tag="ACG8KQ_001016"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9103971.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10162..10311)
                     /gene="rpmG"
                     /locus_tag="ACG8KQ_001017"
     CDS             complement(10162..10311)
                     /gene="rpmG"
                     /locus_tag="ACG8KQ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9103972.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10539..10985
                     /locus_tag="ACG8KQ_001018"
     CDS             10539..10985
                     /locus_tag="ACG8KQ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9103973.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11180..13075)
                     /gene="asnB"
                     /locus_tag="ACG8KQ_001019"
     CDS             complement(11180..13075)
                     /gene="asnB"
                     /locus_tag="ACG8KQ_001019"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9103974.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13400..14374)
                     /gene="bsh"
                     /locus_tag="ACG8KQ_001020"
     CDS             complement(13400..14374)
                     /gene="bsh"
                     /locus_tag="ACG8KQ_001020"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9103975.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14708..14795)
                     /locus_tag="ACG8KQ_001021"
     tRNA            complement(14708..14795)
                     /locus_tag="ACG8KQ_001021"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14761..14763),aa:Ser,seq:cga)
     gene            complement(14940..15506)
                     /locus_tag="ACG8KQ_001022"
     CDS             complement(14940..15506)
                     /locus_tag="ACG8KQ_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9103976.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            15762..17092
                     /locus_tag="ACG8KQ_001023"
                     /pseudo
     CDS             15762..17092
                     /locus_tag="ACG8KQ_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            17058..17408
                     /locus_tag="ACG8KQ_001024"
     CDS             17058..17408
                     /locus_tag="ACG8KQ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9103977.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(17500..17919)
                     /locus_tag="ACG8KQ_001025"
                     /pseudo
     CDS             complement(17500..17919)
                     /locus_tag="ACG8KQ_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(17920..18264)
                     /locus_tag="ACG8KQ_001026"
     CDS             complement(17920..18264)
                     /locus_tag="ACG8KQ_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9103978.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            18530..19489
                     /locus_tag="ACG8KQ_001027"
     CDS             18530..19489
                     /locus_tag="ACG8KQ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9103979.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            19966..20166
                     /locus_tag="ACG8KQ_001028"
                     /pseudo
     CDS             19966..20166
                     /locus_tag="ACG8KQ_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20878..21483)
                     /locus_tag="ACG8KQ_001029"
     CDS             complement(20878..21483)
                     /locus_tag="ACG8KQ_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9103980.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            21665..22000
                     /locus_tag="ACG8KQ_001030"
                     /pseudo
     CDS             21665..22000
                     /locus_tag="ACG8KQ_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            22165..22380
                     /locus_tag="ACG8KQ_001031"
                     /pseudo
     CDS             22165..22380
                     /locus_tag="ACG8KQ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22518..23489
                     /locus_tag="ACG8KQ_001032"
     CDS             22518..23489
                     /locus_tag="ACG8KQ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9103981.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            23629..24942
                     /locus_tag="ACG8KQ_001033"
     CDS             23629..24942
                     /locus_tag="ACG8KQ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFP9103982.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            24985..25851
                     /locus_tag="ACG8KQ_001034"
     CDS             24985..25851
                     /locus_tag="ACG8KQ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103983.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            25848..26696
                     /locus_tag="ACG8KQ_001035"
     CDS             25848..26696
                     /locus_tag="ACG8KQ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9103984.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            26710..28182
                     /locus_tag="ACG8KQ_001036"
     CDS             26710..28182
                     /locus_tag="ACG8KQ_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP9103985.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            28199..>28441
                     /locus_tag="ACG8KQ_001037"
     CDS             28199..>28441
                     /locus_tag="ACG8KQ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9103986.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         8782 a         5507 c         5146 g         9006 t
ORIGIN      
        1 gaaaaaattt gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg
       61 tttggttgct tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat
      121 cgaagaaaac cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa
      181 tacgcatgaa gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc
      241 aatataagtt gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta
      301 gctactgctg cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca
      361 gactggattt cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct
      421 tctgaatgat aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc
      481 ttcaaatttt ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct
      541 tcaatcaaat agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc
      601 ttttgctgaa tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct
      661 gctaattctt tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta
      721 aaccatcccc atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc
      781 tgccggattg gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg
      841 caattgcctt tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa
      901 aagggctcat tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg
      961 attagtgctg gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca
     1021 aaatttttct ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac
     1081 tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc
     1141 cattgctcca ggttttcatt tgttagtaac cgtggccatg ccattgctcc gccttgcttt
     1201 tgatttcttc ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt
     1261 tccttgcctg atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt
     1321 gatactgtaa acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca
     1381 ttttctttag gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac
     1441 agtaaaacat ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt
     1501 tcccagtgac cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt
     1561 aaaagataac catcttttct tttgcttact ttttttacta aagaataaac aaactgaata
     1621 gaagctggaa attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa
     1681 catcgagtag attgctgatc tttttccaga cgattgattt cttctataat tagttgctta
     1741 tttttggtta ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag
     1801 ttgggtccct gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact
     1861 tgttgagaaa cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct
     1921 ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg
     1981 agaatccttt ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt
     2041 tgcatgcctc catttcgata aattttttca ataaaacagc atggttctga tgttcatttt
     2101 ttgcaccata aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa
     2161 tagcttgctt cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag
     2221 caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt
     2281 tcaaccacag atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca
     2341 cgagtattct atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca
     2401 tagttgtttc cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa
     2461 taaaaaaaat gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct
     2521 aatgattgat tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt
     2581 tgatttttcc agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg
     2641 ctttacgtaa tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga
     2701 caggctggtc ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc
     2761 ttgcacctaa aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg
     2821 cttttctcat tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca
     2881 ttactcgtag atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat
     2941 taattaccca taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga
     3001 ggcagcacca atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc
     3061 tcttacttgc gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc
     3121 tcctgctgcc gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa
     3181 gtttccgcaa gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg
     3241 atcgtcttct tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag
     3301 cattgctttc agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac
     3361 cacacctgta gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa
     3421 cccttcagga tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat
     3481 caaatttccg ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac
     3541 gtctggatcg ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc
     3601 attatcgata gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac
     3661 ttctgtccag tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc
     3721 tggggtaccc atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg
     3781 atgattgatt gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt
     3841 atcgatgctc catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt
     3901 tgttccacct aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg
     3961 ctttcttcaa ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga
     4021 tcaatcaaac ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag
     4081 atgcgtgcac ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat
     4141 aagctctcca tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac
     4201 ccagtaaaat acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt
     4261 gtatcgaata gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc
     4321 caattagccc accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa
     4381 atgtcaacac aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt
     4441 taaaatgaaa ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac
     4501 caccagacag aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc
     4561 tgaacatata aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca
     4621 taaaatacag tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg
     4681 gtgtcactaa acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat
     4741 aataagggat ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc
     4801 ctaaaaaagc ataggtccat aacggtcgtc ttaaccatac acgcaatttt atttgctgtt
     4861 gataattcat gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga
     4921 tcaaagctct ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt
     4981 tttttatgaa aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa
     5041 ccggctctat tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag
     5101 agcttctctg attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca
     5161 tccatttcat aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt
     5221 tgttcttttt ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa
     5281 gtcgtcccta tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt
     5341 cgctgttctt tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct
     5401 ttccggcgta atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat
     5461 ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa
     5521 aaagaagacc tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt
     5581 gtttttgtct gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa
     5641 ttcctgtagg tacttcccca cttagtagaa tcactctagc aaccatatct gcactaagca
     5701 gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga
     5761 ctagcttcgc agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta
     5821 cactaccgct tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt
     5881 taaggcctaa tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat
     5941 aaagtcctcc tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc
     6001 agctcgcacc ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag
     6061 ataattggat cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg
     6121 acaataagat tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac
     6181 agcaagcagc taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat
     6241 agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca
     6301 atatactaga atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc
     6361 caagacaagc tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa
     6421 tcagcgccaa agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa
     6481 taaaaggcgt accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga
     6541 aatagaacgt ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa
     6601 cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat
     6661 cctcgctgta aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag
     6721 aacataacga tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat
     6781 aaatgaaaca ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa
     6841 aacaatcaac cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa
     6901 atgaatcaaa gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa
     6961 aggaaacaat actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa
     7021 agcaagatcc tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa
     7081 agaactaata gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa
     7141 ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa
     7201 cacagaaaaa tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc
     7261 acccgcgttg attcctagta agcctgaatc tgctaaagga tttcttgtca ctccttgcat
     7321 caatgcaccg ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa
     7381 acgaatattt cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg
     7441 ccaaaaatct gcttttccca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat
     7501 caaaagaaca aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga
     7561 tcctcctgct cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga
     7621 agcgtgacaa tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact
     7681 tcttcaggtg taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa
     7741 aaacgtgaag catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga
     7801 ttgatttcag tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc
     7861 ggttcatcca agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc
     7921 tgggtttgtc caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta
     7981 gcatccaacg cccactcgat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt
     8041 tgataagggt atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca
     8101 ctagtttgtg ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata
     8161 tcagagttat tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc
     8221 aataaagtag attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga
     8281 atcgtaacag acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct
     8341 gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct
     8401 gtttcaacat ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc
     8461 gttcgaaata tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag
     8521 aaatgaaatt aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac
     8581 tgagcgcttg ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt
     8641 catccaatag tactcataca gtcacagata cacttggaca taaagtagag attccaaacg
     8701 cgcccaaacg gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac
     8761 ctatcgccca gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa
     8821 aggatgtccc aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg
     8881 atttgctgtt gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca
     8941 aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa
     9001 aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga aaagatcatt tccgattatc
     9061 aaaaatccat caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg
     9121 cagtgttgtg ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa
     9181 gaatcgtata cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa
     9241 aagccacttc tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact
     9301 acatcatcct tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga
     9361 atcttaaagc agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat
     9421 ataacggacc tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat
     9481 tttaactaaa aaaagcatct tcacgattag tcgtgatatg ctttttttct tattaacaaa
     9541 ctagacgcat agattttatt cttcttttga agcaacccta ttttttactc tttcccattt
     9601 ttgaaaacga tgggcatata gattttcttg atccgttatc ccctcatact cctctataag
     9661 ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat
     9721 caatgttcgc cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc
     9781 cgaaacatag atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg
     9841 cattacttcc gcattttctt tcgtgcgaaa atttggctgc gttgtcaaca cgatgatatg
     9901 gcgataagga agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat
     9961 tcgatccatt gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa
    10021 accatttttg ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc
    10081 cgctccttat gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag
    10141 gaattgagaa ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg
    10201 tgggcaatat ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag
    10261 atagttacgt tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc
    10321 tcccattttt acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt
    10381 tttctagatt gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac
    10441 aacggtttta cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt
    10501 tatatagatt atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact
    10561 ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa
    10621 atgcattatt agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag
    10681 caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa
    10741 atccaagcaa gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag
    10801 atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg
    10861 caagcaacca ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa
    10921 acaaagaatc aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt
    10981 attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa
    11041 caatggtgtt cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag
    11101 ctgttttttg aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt
    11161 tattgatatt tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg
    11221 tttcatctac aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc
    11281 tttgctcttc tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat
    11341 caaaaaattc ttttgcaaaa tctgcttcga acaatgcttt gacttgttcg tagccgtgtt
    11401 cttctttcaa ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt
    11461 tttccggaag atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca
    11521 ataaaaactt acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta
    11581 ctcggacttc caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg
    11641 gcatccattg atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg
    11701 ttttctcgta atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg
    11761 ttaacacttc tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt
    11821 ttgcaggagc aagtgattca tataaatgga tccgtccatg gaaatttttc atttttccga
    11881 ttttacgtcc taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca
    11941 cacggatgaa ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat
    12001 ccgccccttc tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt
    12061 acaaaggtac gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga
    12121 atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt
    12181 cggttagttt tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga
    12241 aagaatgatc tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga
    12301 aagaaccaac ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt
    12361 cgattttttc gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc
    12421 aatattgctg aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac
    12481 gatagacatc tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag
    12541 gtttcaacgc ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga
    12601 tttctgaacc aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa
    12661 agtggtctct tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca
    12721 ttccgcggat cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt
    12781 ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct
    12841 ggtaattata gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag
    12901 gctgagtacc gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt
    12961 tatcgatata ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat
    13021 taataattgt ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac
    13081 tttcacatcc taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata
    13141 aatagcggac gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt
    13201 ttctgttttt acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg
    13261 ctaccttatt ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc
    13321 aaaaaaagac acgacctaaa gatcgtgtct aattatgact gtatcagaaa atatgatacc
    13381 aaacggatct attcattaat taattgacat agtttatctg ttgctctttt atgactggat
    13441 aactgaccaa ttcatgacga tccaagtctt ctttattcat atttacagca gtaatttgac
    13501 tgtcttcata cgtacgataa taataaatcc ctttatcaac gttacagcaa gaagaataaa
    13561 tcgtatattc gtattttctt tctccagtat cgcatactcc tttttgttgc tcgactgagc
    13621 ctagtatatg aaaaaattga ctgatacttt cagattctga atcacctgat gcagcattca
    13681 ttttagtaaa tgtggctttt acaaatcgag aaacagaaga gagatctcca ggtaagccca
    13741 atcctcccat cccacgacta taaacattca agctgatctg gtttgaaaaa tgattttctg
    13801 gtgttccatt tgatagcgag cgataattgt tcagattaaa taattgataa tcaaatggcg
    13861 gattatttgt caacacaccg acaggattat catataaatg aagtccatct ttcatacttt
    13921 caattactat cgatttttct ttatcagcta ataaccagtg taaaggtgac aagggtagtt
    13981 tttcactaaa atcgatattg actagattga tagtagccaa taattcttta gcttcgtcta
    14041 tggttgagca ttgccctagt atccatggaa taaactcaaa tggtgcaacg ttatcttttc
    14101 cttcttgtaa ttctttataa tcagcattgc caggaaaatt cagtcctgcc atactcaacc
    14161 ctttttcatt tgtagcctca taatagagtg gatagttgga taccccagct gctattccta
    14221 tcattgcata atgtttatct aaatttttta cctttctaaa atcgaaacga taatttcttg
    14281 gagtaatagt gaccacttca ttataagaca tctcataatc aaaattcctt ccaaaataat
    14341 ggtctttcgt tgcataagtg attgcggtac acatcgtttc ccctcctaaa acaaatcgtt
    14401 tttacacttt tatcttacat ctagtatttt ttttatcaaa aaaaggccaa agaagattga
    14461 tttactagca tttataaaaa atagtactca gggggaatca aaaaatattg taggttaaaa
    14521 gtacaacaat aaggggctgt gacaaaagtc ttgtcacagc cccttattcc gaacaaacgg
    14581 tgttcaaaag cttgcatctg cggtaataag cccaatcaaa cgaaaaaata ggaaaagcta
    14641 tttttgtttg gccgttctta ttacttgatg caggacagct tcttcacaac ctctaatctt
    14701 tgatataagg aaggtacagg atttgaacct gcgcgccggt attagccggt tcgccggatt
    14761 tcgagtccgg tgcattacca ctctgccaac cttccgtaag tactattttt aaactttttt
    14821 cacgaaatgt caagcctatt tctaatagat aaataacaga aatcgctttt tttagcaaaa
    14881 aagtccattg acagtttgca gccaacggac ttaccgatca aattcatact gatagaacac
    14941 tatctgatta tttcaggatt atctaatgct aaaatcgagt caattgtccg tttgatcagt
    15001 gcaatgattt gttcttctgt tggatgcatg atgtttgatg acatcaatga cgctaagcca
    15061 gtcactgcta cccatgtacc agtatgcaga gcatcgacat attcatccga taattgattg
    15121 tatctttcat cttctttaac tatttcacgg aaataattgt atgaaaaatc atgcattttc
    15181 tttccgtccc catgttcatc aatgtacaat gccatgtaaa gactatgatg cttttttgca
    15241 aagtagatat agttcaaacc gagatcaatg attttatcac cagtacgttc aatagggaaa
    15301 acatcttctt ttagttgttc aaagatagaa tccagcaacg tattttttaa atcttccatg
    15361 tttttgaatt ccagataaat cggctgtgtc gaaatcccca tctttttagc aatatttctc
    15421 gcagtaaatc ctttaaaccc ttcctttgca acaacatcat aagcagcttt tagaatttga
    15481 tccttggtat agacttttct tctcattaaa actcccccac ttctttactg cagtatatgt
    15541 tgttttattg taccctattt ttagcatttt ttgcaaacat tttactcaaa acgttatatt
    15601 ttaaattttt tttcagtctt ctttaacttt tttttgcaaa attaaaaaaa tttggttaaa
    15661 ttcaatgggt tcccttacat ttttagtttt gacctaaatg atagttgtcc aaatctttca
    15721 tttcaagtta tagttaaaga aaatgaaaaa gaaggtgtgt aatggaaaac gaaactgacg
    15781 ttatttatat tcatcctcaa aaacgaatcg tttcacagaa aagaaagtat ttttatcttg
    15841 gttttacagg tgtttttttc tttttattgg cttgctttct aatactccta ctgataattg
    15901 gagtggattg ctaactattc ttacctcgcc aagcaatctt ttgacagatt attttgcttt
    15961 aggcggtttc ggaagcgcat tcataaatgt agggattttg accttacttt ctgtgctttt
    16021 agcttatcgg cacaaggtga ttttaaacgg tcccttattt gcttcgattc taacagttac
    16081 cggtttctct ttttttggca aaaaccttta taattctatc tcaatcattt taggtgtgta
    16141 tctatacgct gtatttgtca ataaaccttt ttctcagtac atcatgatcg gactgtttgg
    16201 tagcgcatta tcaccggtcg ttagttatat cacttttggt atgcgttttc ctttactgat
    16261 aggtatttta ttggggaatt tagctggcat agcaattggt ctacttcttc cgcccttagc
    16321 tgctcaaact ttagttttcc atcgtggatt cacactttat aatattggat tcacctctgg
    16381 gttgattgcg atgactttta ctgctgtttt gaggttattc agctattcaa tcgtcgaaaa
    16441 tacattggtt ttcaatgaat atcactttcc tctcatttgg attatttttg gctttttttc
    16501 gctgacagtc ggaattggtt tctattataa ttcctttcgg cttttgggga ttcgagaaat
    16561 tttcgattca tctggaaaat tgacaactga tttcattgct aattctggta ttggtgctac
    16621 tttgatcaac ataggcttag ttggtctgat gctgtcttct tatgttttgc tggttggcgg
    16681 tcagttaaat ggacctgtca tcggtgctat tttatctgcg gtgggtttca gtgcttttgg
    16741 ttgtcattta aaaaacagtt ttccgatact agtcggaata tttattgctt ctttgtttgg
    16801 aacttttcat gagatcacca gtactggcat gcttgttgca gctgtgtttg ggacaggtct
    16861 cgcgcccata agcggttttt acggcagttt ttacggtgta atagctggtg tactacatat
    16921 tgctcttgtc cataacgtca gcactttgca cggaggacta aatctatata acagtggttt
    16981 ttcaactggt ttcgttgcag gtatccttgt accgatacta gataacttta ccgctgtcag
    17041 aaaggaaaaa aagacacttg gaaaaagaat tatcaaaaag aatcatcgat gagcttctta
    17101 cttatttata ttcccatgaa ataactgaaa tacgtattgg agtagaattt gctgaggaag
    17161 ggcttttcat agagatacag ggtacggcag aaactcagcc agaagattta gatgtacttg
    17221 ctgatttgct gaacaatcct cgggacccta gtatggaaat ctattatcaa gaactattag
    17281 gtaatccccg acatgatcgg gaagattatc atctgttagg tagtttgatt gatgacgtgg
    17341 aaatcatgta tgcccagccg attatcaata ttaagatctt tcgacgtgct tctcattcct
    17401 attattaacc ctattttcta tttataaaag actgaatcaa aaaagcagct cctgaaagtt
    17461 cgattaaaat ctactttcag gagctgttca agcttgacat tattgatttt ccaatccttc
    17521 atacacttca tctgttacag gttctaaaaa ctctgaatta ccagcagtga tcgccaaatg
    17581 ttcaaaccaa ctgtcttttg tcgcaccatg ccaatgttta atccctttgt gcgttacgac
    17641 gacatctcca gccattaatc gtcttgctgg ctttccttct tcctgatacc agccttctcc
    17701 tccggtcaca agtaagattt gatagccgtc tttatggaca tgccagtgat tgcgacaacc
    17761 tggttcaaaa gttacattac ctacaccaac agaaacttcg ggatcattca ctaacatatt
    17821 cagatagctt tacccggtaa atacttgagc gtaagcttcg tttttttcac ctgctggaaa
    17881 aatcacgcca ttgtgtattt cttcgtgttt tgtcgtcatt tacgcctctt tcttatcttc
    17941 gctattttct tctaaataga tttcttttgc taaactaaaa gctgaccaag cttttggcca
    18001 tcctacataa aaagctaaat gggtaatgat ttctgctatt tcttcactag taatgccatt
    18061 ttgcttgcca atcttcagat gagctggcaa ttgttcaaaa tttcctcctg aaattaatgc
    18121 agatatggtg atgatactac gctgatgagc agataattct tctctggacc atacttgtcc
    18181 aaataaaaca tcatcattca attctgcaaa ttttggtgca aaatctccta attgatctcg
    18241 tcctgcggtt tgtttttctg ccatttctct tattctccct tcacgactgt taggtgattc
    18301 aactatggtt ctaaggcaac tttattttct attgtttctt cgcatttcat tttaaatgta
    18361 gtctttcagc ctgtccaatg cttagtttat tcagtcgata tgctttttaa gcatagttgc
    18421 taacgctttt tttattcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt
    18481 gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac
    18541 ccatcttaca acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt
    18601 tgctaaaact gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt
    18661 cttcaagcaa ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa
    18721 ctgtggtaga cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt
    18781 tgttcaagga tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc
    18841 tgctcgtacc atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat
    18901 gaaaggcaag cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg
    18961 ctctattcat gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga
    19021 aggtgacact atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt
    19081 atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa
    19141 attaaatcaa tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg
    19201 tggaaaggaa ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt
    19261 cgctgatcca ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg
    19321 tagagatggt ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc
    19381 tgtcgcatct aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga
    19441 agtatttttg agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagaca
    19501 tttaaaaaga aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta
    19561 tatttcattt ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga
    19621 ctggggtatt gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt
    19681 ttataatgct tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga
    19741 gaaaaaaaca agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta
    19801 gaatgaaaca cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct
    19861 aagttttttc atatttgttt gttatgctat acatagatct gcataattag acttgcgctg
    19921 cccgttattg aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa
    19981 atatttgaga aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg
    20041 atcacctcct accccactgt tgactagaca tataaggaaa agaaatactt actttggctg
    20101 tgccagttag atatagatac ctttgaaaca tttgaagtga tcctagataa aattaaacag
    20161 aattgatcag aaacaagcga agccttctct cggttcgctt gttttgctat ttattagttg
    20221 gctcagggca actctaagct cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac
    20281 cactttagaa tacatggatg tacgtattgc cattgactcc tattagttac tgaaaaaaaa
    20341 tttaaacaag ttgagtattg cagataaata gctccagtga ggtgaataac tgctccactc
    20401 aaggcaatag atagctccat ccagggcaac aaactgctcc actttcatat ttaatagatg
    20461 aataaccttc tgtataatag aaaggcatac ctttcggtat gacaacttat acagaatgtt
    20521 attttattgg ttcactatcg aaaaattttg gagctttatt tttccggtat ttctcaacga
    20581 acgattagtt taactgtggg aagctccaga catacgattc gagaagttac ttctcgagca
    20641 gacaggaagg gggtgactga actaaccgct gatatgagcg atacttggct gtaggaatat
    20701 ctttttccgg aaaaacaaac aacagcacga ggttaccttc ctgtagactg ggaatatgtt
    20761 cataaagaac taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga atatggggta
    20821 catggaacat agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt ataaattcta
    20881 caaatcattc gctttcttta ttttatatat ggttggtcga gagagaccat tttctttggc
    20941 aatttctgca actggaactc cttgatttaa catgtccacc acagttttaa agactaatcg
    21001 tttttgcgga tctgttgagt tctcagaata ttcaattggt cgccccttat atcgaccagc
    21061 ttttttggcc aataatactc cttgccgctg acgttcacgg attttctcac gttcgttttg
    21121 tgcaatataa cttagcaatg ataaaaacat atcgaacata gcagtatcta ggagttcatt
    21181 tccagtatta aaatctaaaa actgtgcatc tagtattttt aatgctactt ttttctgttt
    21241 cataaaggta actgtatttt ttatgtcttc ataatctctg cctaggcgat ccagtgatgc
    21301 aacaatgaca caatcacctt ctcggatata atttagtaat ttttgaaact caggtcgctg
    21361 gatatcttta ccactggctt tttcttggaa taatttatca atgttaaggt cttccatcat
    21421 tgccaattgt cgatctaaat tttgatcttg tgaagacact ctaatatatc caatttttgc
    21481 cataaaaact ccttttaaaa aatgatataa tttacatgta aaatttactt gtagttttac
    21541 tgttatcata gcatttttaa aaataaattt taagtctact tataatttac tttggaggtt
    21601 ttgaatgaat gaaattaagc gcaataaaaa ttcttttggt gtttatttag gagagcctag
    21661 ctcggaacag ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata tattttatgg
    21721 tagaaaaggc aaactttatc aaacttatta cgacggtatg gaggaacagc tcaacgcatt
    21781 aggattagta actaatgcta tcatatattg gaattcacta tatttagaaa aagtctttga
    21841 acaaattaaa acggaagggt tcgactgttc cgaagaagtg ttacgcaagc aatttccatt
    21901 aattagtgat cacattaatt ttgttggcaa atatagtttc cgctatgaaa aagaattatc
    21961 gaatggaata ctacgtccgt taaatataga gaattattga gaatgttcgt cttttggttc
    22021 ctaccgtgca ttttcgcacg gatgttccag atactatttt tgaatgtata aaattgaaaa
    22081 cacaagaata ctaataaatc aacgattctt gtgtttttaa tttttcacta ccgtgcattt
    22141 tcgtttctat gttccatgta ccccaatctt gaaaaattaa ttaaagactt agataaagcc
    22201 attgacgact tagtcgtggt gctaccggag tatcaatgtt taaccagtat cccaggtatt
    22261 ggtcctgttt aggctgctgg aatactcgct gaaattggtc caattgaacg tttttctgat
    22321 gaaataaaat tgacaaagta tgccggccta tattggcgac agagtcaatc cggtaaatct
    22381 gaatacgaaa atttttttac gaaaagcttc acgtctaata tctattttaa aataaaaata
    22441 ttcctgctaa tttggaatcg tttccttctt ttggtataat aatacctgaa attcttttga
    22501 gaggagttgt tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta gcaggagttg
    22561 gtgtgggtac ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa
    22621 aagtaatggc tgcaattagt gagcttaatt atgagcctga tgaatatgcc agaggtatga
    22681 aaacgaatag tagcaataca atagctctta ttttacctac gatttggcat cccttctttt
    22741 ccgagtttgc ctactatgta gaggaagctc tcagccaaaa aaattataag ctttttttgt
    22801 gtaatgcgaa cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc aagcagagta
    22861 aagtagacgg aattataggt attacctatt cagacattga taaatatgta tcatcaaatt
    22921 taccatttgt cagtatagac cgccattttt cggaacaggt ttcttatgta acggcagata
    22981 attatttagg tgggcaaatt gccgctcaag aactacttac tcgtaactgt aaaaacttgt
    23041 gctatgttgg gggtgtttca ccttacccta acgaaacaaa caatcgtaaa gctggttttt
    23101 acaatatttg tgaggaaaat aaaatagctg tgaaggtatt ggatatgcct gaaccaatta
    23161 gcaatttaga ggaacaattg ctcgcttttt tttcggataa tcctgaaatt gatagcattt
    23221 ttaccatcaa tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg
    23281 aagcacctaa ggattttcaa attattggtt ttgatggagt caagctatcg ttgcaaaaag
    23341 actatttact atcaacgatt gtacagccga tagaggaaat ggccaaggtt tcagtagaaa
    23401 cactcatggc gatgatttta aatgaagaac cgcctaagcg tgtggtagta ccggttttct
    23461 ttcacgctgg agagacgact aaagagtaaa aaaccggttt ataacgattt ttttgagata
    23521 ggttgacaga aagtgatttc aatgatatct taaaaccaat taattggaaa cgttaccaat
    23581 taattgaaaa ataaaacgga aacgtttcaa aaaataggag gaacatcgat gaaaaagaga
    23641 atgttagcaa ttgcagcaat tttagcatta acagctggag gacttgcagg atgtggcagt
    23701 aaggagaaaa gttcaggtag cagtgatcca ggagaaatta agatttgggt tcagttttct
    23761 gatgaaacgc ctgaaggaaa agcttgggca gagattgtga acaattttaa tgatgaatac
    23821 gagggtgaat ataaagctgt tactgagtat attcctcgta gcggaagcgg cggtggatat
    23881 gaggataaag taaatgctgc aattacaacc aatacccttc ctgatgttat tacgttagat
    23941 ggaccgaata ctgcagctta tgctcattca ggagtcattg ctccattaga tgattatttg
    24001 aaagatgcgg acatggatga tgtattagac agcattattc agcaaggaac ctacgatggc
    24061 aaaatgtatg cgtttggttt ctcagagtcc agtgtgggtg tctattacaa taagtcgctg
    24121 ttcaaagatg ctggaattac agaagatcaa ttgccgacgt tagaagatcc ttggacttgg
    24181 acggaatttc aagcaatttg ccaaaagctg actgataaat acaaaacgcc tgcaattgat
    24241 atgcaaatgg cgtcgggaga cgaaatgctg acctacgcat atacaccatt tatatggtcg
    24301 aatggcggcg atgtcgttaa tgctgatgga accaaagccg aagactattt taacggtaaa
    24361 aaatccgttg aagcgttgga atttattcaa gaattggttg aaaaaggtta caccacaatc
    24421 agtcctattg aaaaaggatt tgaaactggc aaatatgcca tgatgctgag cggttcttgg
    24481 acaatggcag atttagagga aaactatcaa gagattgatt atggtatttt gccttacccc
    24541 gtttcagatg caactgggga gttagtttca ccaaccggaa gctggcagtt agcaatgact
    24601 gagaaaacag agaaaaagga tgcttctgcg gcctttatta agtttgcaac cagtacagaa
    24661 tctagtcgta ttatgagttt agggaacagt gttctaccta tcagaaattc aacagttgat
    24721 ttaattaaag atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa ccaactttct
    24781 ggacatgccc gcccagtggt tgtagcttat ccgcaagtat ctagagcatt ccaacaagca
    24841 gttcaagata ttagctactt cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc
    24901 gctgctgaaa tgcaacaggc aattgatact tcactaaaat agaagtttct aaagggggcc
    24961 aatcaagtgt taaaaaaacc gacaatgaaa gacaatttga taggttacgg ctttctagcg
    25021 ccagctttgc tgctgctgac gatttttctg atcatacccg taatcatggt aatttattat
    25081 gcctttactg attattattt gttaacgcca gatgctagac aatttatcgg cttagaaaat
    25141 tttaagcgac tgttcgctga tccaattttt gtaaaaagta tttggaacac ggctaaattt
    25201 gttgtttgga ttattccggt ccaaataggg gcagctttag gaatggcact tattatcaac
    25261 aaaaatcgta aaggaaatat cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc
    25321 ttagtcgtta tttcgatttt atggttgtac ttgttaaatc ctaatgaagg tctggttaat
    25381 acgctatttg caaagattgg aattgcttcg cagccttttt tgactagtcc caaacaagca
    25441 atgtatacgc ttgttttcgt atcggcatgg caaggtgcag gatatcagat gctgctgttt
    25501 ttagggggaa tgcaaaatat tccgcaagac gtttatgagg cagccgaaat tgatggtttt
    25561 tctaagtttc agcaatttcg ctatatcacc atgccattat taaagcccac ggcgattttt
    25621 attttactta ccacattgat ttcggccttt aaactaatca ttcagccaat ggtaatgact
    25681 caaggtggac caatgaattc tactatgaca atggtttatt acatttacca aactggattt
    25741 acagaccgta tggtaggcta ctccagctcc attgcgttaa tctttaccac tatgattggt
    25801 ttaattacaa ttgctcaaag aaaattggta aaggaggatg atccagcatg accaagaaat
    25861 ttggaccgct aatgatcgta gaatattgtt tgctgttcct tttagcggca ttattcattt
    25921 tcccgatgct gtggatggta gtttcctcta tgaagcctga agcagatgtc tatcaaaatt
    25981 tagcttcctt caaagctttt ttacctagtt tgaatccagc taattggttt aaaacttatc
    26041 aagaggttat ttcccggttc agtgtgggaa cttatttatt aaacagtatt ttttatggtt
    26101 taacctttgc ctgtgggtca atcattgtaa actctttggc gggttttgcc tttgcgaagg
    26161 ttaatttttc aggcaagaaa attatctttg gagctttgtt agctctgctg attgtcccga
    26221 tggagacggt tttaattccg caatttacca tcgttaattc actaggctta gtgaatactc
    26281 gcttggcagt gattttacca gcgatggcca gcgcgtttaa tatctatctg tttagaaatt
    26341 tctttatcgc gattccagaa gaaatcattg aatcagcgaa attagatggt gcaaatattg
    26401 ttaaaatttt ctttcacatc atgctgccaa tgtctaaacc agccatagcc acagtaggtg
    26461 tcttatcctt tatctccagc tggaatgatt atatttggcc gttaatggtc ttaactgaca
    26521 gcagcaaatt ttcaatgcaa gtggcaatta ctacgatcaa tactacacag ccagtttata
    26581 tcaatcaagt gatggctgtt ttaaccattg caacgattcc actgattatt gtttatatcg
    26641 ttgcacaaaa atatattctg cagggacttg gcggttctgg aacgggtata aaataggagt
    26701 gaatagtgaa tgttgcaaaa acttttaaag gccaatgagt ttattgagaa aaatgctcaa
    26761 acagtcaatc aagaataccg gaataaattt catttgatgg ctccgattgg ctggatgaat
    26821 gatccaaatg gttttgttta ttatcaagag gagtatcact tgttttatca atattatcct
    26881 tacgacagca tctggggacc aatgcattgg ggccatgcta aaagcaagga tctaattcac
    26941 tgggagcatt tgccagtagc actggcacca ggtgaaagct atgacaagga aggctgtttt
    27001 tccggcagtg ccatagaaaa ggatggcaag ctgtatttga tttatacagg acacaatgtt
    27061 gtggaagggc aagttcgaca agttcagtgt ttggcagttt cagaagatgg cattcacttt
    27121 gaaaaatatg ccggaaatcc aatcatcgca gaagatcact tagcttctgt tgcaacgacc
    27181 gaagatttcc gcgatcccaa agtgtttcag cgaaaagaag actattttac tgtagtcgct
    27241 actaaaactg cagatgctcg tggacaaatt gtgatgttcc attccaaaga tttactaact
    27301 tgggagtttt attccgtatt gctggaggga actccagaac aaggcattgt atgggaatgc
    27361 ccagacttgt ttgaactaga tggcaaagaa gtcttgattc tttctcctat tcaaatgact
    27421 ccacaaggca atgcttacca aaatacgaac tcaactgttg ctttcatcgg tactgtcgat
    27481 tggcaaacag gcaaatttct agtagagaat tttcatgaaa tcgatggcgg tttagatttt
    27541 tatgcacccc agacctgtga aaacgaagca ggccaaagaa taatgattgc ttggatgcaa
    27601 atgtggcagc gtaaaatacc tacgaatgat ctgggccatg gttggtcagg ttccatgacc
    27661 ttgcctagag agctgagggt ggctgataat caattgttgc aacggccagt ttcatcaatt
    27721 tatcaagtaa ttgatccgca ggaaaggatt gaatgggcga agactgaaga gaatcgcttc
    27781 ttactgaagg acttagtata tgaccagcac tatttacgct tggaaatcaa tttaaaacag
    27841 gcaacgaaac tagaacttca ctatgcgaag ggacccgacc atacattact gcttaactat
    27901 gatgttgaac gggagtgctt tactgcttcc agggaacaca tgccgtatga tttatcagga
    27961 gctgaaaagg aagtgttgaa aaagcgcagt gtgacgattc ctttggaaaa taattgtcta
    28021 accatcgaaa ttttccgtga tacttcagct attgagcttt ttctaaatga tcgtctgact
    28081 atgagtacaa ctttttatga gacagaaaaa ggacaggatt ttcttttgct ggcagacagt
    28141 gaagtaacct tttcattgga aagcggctta attaatctat agaagggaga gctcagtgat
    28201 gatttatggt tcaatcgaag caggcggcac aaaatttgtg tgtgccatta gcgatgaaga
    28261 aatgacgatc aaggaaagag tgagctttcc gacaacgaca cctgaggaaa caataccttt
    28321 agtcattgac ttcttcaaac aataccaagc agatttagca gggattggga ttggctcatt
    28381 tgggcctatc gatatccatc gcgattccgc cacttatggg tacattacct cgaccccgaa
    28441 a
//
LOCUS       DAZKCE010000027        26717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000027 DAZKCE010000000
VERSION     DAZKCE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.027"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1063
                     /locus_tag="ACG8KQ_001038"
     CDS             191..1063
                     /locus_tag="ACG8KQ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9103987.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1060..3018
                     /gene="nagE"
                     /locus_tag="ACG8KQ_001039"
     CDS             1060..3018
                     /gene="nagE"
                     /locus_tag="ACG8KQ_001039"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9103988.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3350..4201)
                     /locus_tag="ACG8KQ_001040"
     CDS             complement(3350..4201)
                     /locus_tag="ACG8KQ_001040"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9103989.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4324..4791
                     /locus_tag="ACG8KQ_001041"
     CDS             4324..4791
                     /locus_tag="ACG8KQ_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9103990.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5054..5536)
                     /locus_tag="ACG8KQ_001042"
     CDS             complement(5054..5536)
                     /locus_tag="ACG8KQ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9103991.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5636..6499)
                     /locus_tag="ACG8KQ_001043"
     CDS             complement(5636..6499)
                     /locus_tag="ACG8KQ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9103992.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7097..7972
                     /locus_tag="ACG8KQ_001044"
     CDS             7097..7972
                     /locus_tag="ACG8KQ_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9103993.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8117..8269)
                     /locus_tag="ACG8KQ_001045"
                     /pseudo
     CDS             complement(8117..8269)
                     /locus_tag="ACG8KQ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8537..9868)
                     /locus_tag="ACG8KQ_001046"
     CDS             complement(8537..9868)
                     /locus_tag="ACG8KQ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9103994.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(10017..10730)
                     /locus_tag="ACG8KQ_001047"
     CDS             complement(10017..10730)
                     /locus_tag="ACG8KQ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9103995.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            10905..12329
                     /locus_tag="ACG8KQ_001048"
     CDS             10905..12329
                     /locus_tag="ACG8KQ_001048"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9103996.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            12628..13335
                     /locus_tag="ACG8KQ_001049"
     CDS             12628..13335
                     /locus_tag="ACG8KQ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9103997.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            13322..13645
                     /locus_tag="ACG8KQ_001050"
     CDS             13322..13645
                     /locus_tag="ACG8KQ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9103998.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(13734..14771)
                     /locus_tag="ACG8KQ_001051"
     CDS             complement(13734..14771)
                     /locus_tag="ACG8KQ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9103999.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(15092..16075)
                     /locus_tag="ACG8KQ_001052"
     CDS             complement(15092..16075)
                     /locus_tag="ACG8KQ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9104000.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(16490..17260)
                     /locus_tag="ACG8KQ_001053"
     CDS             complement(16490..17260)
                     /locus_tag="ACG8KQ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104001.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(17253..18188)
                     /locus_tag="ACG8KQ_001054"
     CDS             complement(17253..18188)
                     /locus_tag="ACG8KQ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104002.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(18504..20153)
                     /locus_tag="ACG8KQ_001055"
     CDS             complement(18504..20153)
                     /locus_tag="ACG8KQ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9104003.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(20270..22873)
                     /locus_tag="ACG8KQ_001056"
     CDS             complement(20270..22873)
                     /locus_tag="ACG8KQ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9104004.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(22885..23934)
                     /locus_tag="ACG8KQ_001057"
     CDS             complement(22885..23934)
                     /locus_tag="ACG8KQ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9104005.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(23960..24274)
                     /locus_tag="ACG8KQ_001058"
     CDS             complement(23960..24274)
                     /locus_tag="ACG8KQ_001058"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9104006.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(24290..24736)
                     /locus_tag="ACG8KQ_001059"
     CDS             complement(24290..24736)
                     /locus_tag="ACG8KQ_001059"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9104007.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(24737..26659)
                     /locus_tag="ACG8KQ_001060"
     CDS             complement(24737..26659)
                     /locus_tag="ACG8KQ_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9104008.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         8118 a         5384 c         4475 g         8740 t
ORIGIN      
        1 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
       61 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
      121 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
      181 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
      241 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
      301 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
      361 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
      421 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
      481 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
      541 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
      601 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
      661 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
      721 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
      781 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
      841 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
      901 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
      961 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
     1021 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
     1081 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
     1141 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
     1201 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
     1261 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
     1321 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
     1381 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
     1441 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
     1501 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
     1561 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
     1621 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
     1681 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
     1741 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
     1801 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
     1861 aacaaagcaa aaacagcttc cttgatgtta gcagcagctt ttgcttcatt ctttactggt
     1921 gtaacagaac cacttgaatt ctcatttatg ttcgttgctt ggccattata tgtcttgcat
     1981 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
     2041 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
     2101 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
     2161 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
     2221 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
     2281 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
     2341 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
     2401 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
     2461 gttgcagatg agatggcaaa actacatggt ggtgctgcaa cacgacctgc acaaacaaac
     2521 accgtggtta aaacagaaac atttgccggt ggaacgatcg aacaagatat ttacgctatc
     2581 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
     2641 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
     2701 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
     2761 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
     2821 tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
     2881 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
     2941 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
     3001 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
     3061 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
     3121 aactccgcgg taataagtcc agccaaacgt aacatatgaa gagctatttt ttatttgact
     3181 cttcttatta cttgatgcag aacagctttt ttacaacctc tgataaacgg tgttcaaaaa
     3241 ccagtttatc ggtatgaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
     3301 ttttcttcat actcaaaact aatcggcttt ttcacaacct ctttatacat tattcctgat
     3361 ccaatatttc ttttaatgct gcttcaagag aagttaatgg ttttcccaat actttttcta
     3421 aatcatcaga ggaaatagct aactgatcct ttttaatatc atgttggatc gctaaaaaca
     3481 tgtcagctgc ttcttgaggg aatccggctt cttttagatt ttcgacaaac gctgcatcac
     3541 ttgcttcaat aatttctaac tttttacctg tcgcacgctc taatgccttg gctaattctt
     3601 catagtcagt tagattgccg gataattcta agatttctgg agagtcactt tctactagta
     3661 cttttgctgc agcttctgca tattcacgtt tcaataccca acctgttttt ccatttcctg
     3721 cagcataaac gaattgacca gtttttaaag cctgcccaat gataggtaat tcattctcta
     3781 cataccaatt attccgtaaa aatgtatggg aaatacctga atccttaatc attttttcag
     3841 tgaattggtg atctggcgct aatacactga tcgagtgatc ggcatctgca aaacttgtat
     3901 aagcgatata agatacccca gctgcttgtg ctgccttcac tacattctta tgttcggctt
     3961 gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc tattcctttc aacgcttctt
     4021 tcattgagtc taaatcatca taatcaccaa tacgtatatt aaatccagct tctttcagtg
     4081 cttctccttt ttcttcacta cgtactagta catagatttc attttgtgga accatctttt
     4141 ttaagaattc cagcgcataa ccaccaaaac ctccagttgc tcctgttact aaataatgca
     4201 tatttgccac tccctattct ttttatttgt taagatttac agctgtacat ttataaatta
     4261 cacctttatc ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa
     4321 ctgatgaagt attctctgca atttagtgat gcaatccatg tacttgccta tattgaaatg
     4381 tttcaagata cccgatcact ttctagtgag atgattgcca gtagtatcga aacaaatgca
     4441 gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc
     4501 ggaaaaccgc agccttccct tgccaaaagt ccagatcaga tttctttact agatgtctat
     4561 cgcagcatcg aaggtaatac acagcttatc caagtcgatc caaaaacaaa tccaaattgc
     4621 gtcgttggtg cgaatatcca agaagcttta gaagaaagct acgctcggct gcagaaaaag
     4681 gcagaagaag aaatggctaa gatcacactt gatagtctga tacataaaat agccgttttg
     4741 gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga gatttttata attctctcta
     4801 ctgtgcctgc atttttctga tcggaatcgt ctacataaat tcttttattc tccgggggac
     4861 aaaagccttg tcatgcccct ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg
     4921 acgtcttgat aaacggtgtt caagtagctg accctcggca ttagttctga aaaatgcaaa
     4981 atatggaaag ctatttccca tttttcctct ctaatgctca ggtcaaagcg ctactttcac
     5041 gacctctata ttatcatggt ttattgacta ccggtatcca gatttctccc atatatttcc
     5101 cgttggtaac tcccatttcg actacagcat ttggaccccc aacataggcg taatcttcta
     5161 cttctcttaa aacatgccca aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg
     5221 attctgctaa agcgattaca acaagatacg ttccttttgg gaatgggatg attcgggtag
     5281 tgtttggaac atcctgactc gtttgaacac cgacatagta catcattttc ccgttatagg
     5341 gttcgttgac tgtaaagaaa taatgatttt cagcagcttg tttcaatgca tctattgttc
     5401 cattaccgat aactttttgc tcaaatgctt gtttctctgc agccaaaccg acaaaatccg
     5461 tattgctact cttcaattcc acaccaatac ccgtcaccgt aaaatctggt ttttcttcga
     5521 tcctatactt tgccatttct cccacccttt ctattatatt aagttcatct tacataaaat
     5581 taaagggaaa tgacacgatt acgctctttt ttctagggat atttttattt tttaactatt
     5641 tttttgcttt cactgcaatc caaatctcac tgtaaacatt actgaaatcc tctccccatt
     5701 ttgtaaaaga gatctcggga gcttcgacta actcataatc tgaagaagga agccattctg
     5761 catagatttt cccccaagta tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa
     5821 tggcccaagt gtgttcttct atagcaagca tttctaaatc agcgtacgga ttttcttttg
     5881 tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa
     5941 atccataaga aacgttgatt acttgatttg gatataaatc tcctaactta tgcatctctt
     6001 ccctttgagt ctccgtgatc gtctgcgcta acttttgtat ttcttgattg actccttcaa
     6061 atttgacagg aacttttttc gccacacctg ccacataaaa ctgttctttc ttttcgatac
     6121 gaaattccat cgattttcct cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt
     6181 tttgtatgcc gtatctgctg atttctgaag gcagatatcc tgaccaatca cgaaaagctc
     6241 ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc cgtttctgtc actcttgatc
     6301 catctaggag atcgcgatta gccaaagcca atctacggtt tttgatatag tccattagcg
     6361 acatccctgc aataaacgta aagatgcgtt ttaattgata ttcagagatt cctgtatttt
     6421 cggctattat tttataggaa agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt
     6481 ggttcagatc tttaagcata tttatcttcc tccttgcttg cctttttatc atattatcaa
     6541 agaaagtcaa ttacttgatg aatcaggaac caatttagtc agatgtagaa aatgttgtat
     6601 ggatctaaag aaaattctca agcagcgttt cgtaacggat aattggtatt atctgcctat
     6661 ctatgtttta cctttagaaa aataaatttt ggggaagaca cattctaagt aaacaaagcg
     6721 gtaaaaatat cttttattcc cttagttaat ccgcttacta cctactgtac aaagcaaatt
     6781 gttgttcaaa ctgctgatca gaacgctgct tggatttttc tttgcgatac atcatacctc
     6841 ttttatcaat tggaacaatt atttatttct aacaaaatca cttcattttt gtcagataaa
     6901 taaaaaacaa aaaaagttcc atctttaaaa cacgctattc ttttactttt tctcacattc
     6961 tctaagaaaa gaaaaggaga atgaattttt gacaattcga gcaaaagtga ttatcaaata
     7021 tgtaaaatca gctacttttt tcttaaacgt ttctttatac taaatataaa taagtgtttc
     7081 atgaaaggag aactagatga ataattggct tgagtatttt cctgaaaacg ttttggaacg
     7141 cggttacagc tatcatctgc acggttttgt ccgtcatttg aactatacaa gcaagtactt
     7201 gtccgcaaca gtttctggca cggaagatta taaggtagtg atcacttggg atgaaaagac
     7261 caacatgact tgtgattgtc tatatgccat agaaggaaaa aaatgcaaac acatggctgc
     7321 tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc aactactcac tttccgaact
     7381 ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg gagcttttaa cagagattct
     7441 tatagaacat ccgcaattta tcgaacgctt caaagttaag atgccatttc atgcaataaa
     7501 ttattcagat aaattaacaa ctatcatcca taaatacgat catattatca agaaaaacaa
     7561 aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag ttcatccaag aagcagttga
     7621 atcgctcatt cagcaaaatg cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc
     7681 tactctcgaa acattctatt gggagcctga ggatgaacga acccttcttc tgatagagga
     7741 ctgttattat ttatggaaag aattattagc agaagcacct catgcagaaa aaaggcaaat
     7801 gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc tcttattcaa aacgctactc
     7861 cataaaaatc ctgaaagaag actttcggga aaaagaattt tctaaccaaa agaaaaaaat
     7921 agataaaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa
     7981 cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa aaacacgaat
     8041 catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca tttgactatt
     8101 aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctggg tagattttcc
     8161 ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt tccactgcat
     8221 ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac tctaatcttt
     8281 aaagataaat attaaaataa cgattaataa tattaacagt ctttattttt caccaccaaa
     8341 aacctatctt tgcatatact aaatagacgt atatacacag ataggtttgt cttgtgaaaa
     8401 ataatgatta tccttaccaa aaaatgtttg tttttctaca tgttcgtact tatcaaaaaa
     8461 tatgatcctt attgaaatta ctggctattt atattccaac gacttcttgt gttaattttt
     8521 gttatctcat agtaaattat gtttcatttt cacttaattt agttgtcacg tgttcgtcaa
     8581 ttcgtaaaaa cggtaaatat ataaacacac ttgcggctat aattaatatt tgtaaaacag
     8641 ctgctcgcca gtctccggct gttgctaaaa atcccgatat aactggtggc gtagtccaag
     8701 gaatttgcac tactacatga ttaattaatc ccacagcagt tgcaaaatat gctactgtca
     8761 aagaaaaaat cggtgcaagc acaaagggaa taatcagact gatattaaat acaataggca
     8821 atccaaaaat aataggttcg cttatattga atatagcggg agttaccgat aatttagcaa
     8881 agtccttata atcttttcta cgactgaaga taaatattgc aatcaataaa gcaatcgtat
     8941 ttcccgatcc acccattacc gcaaaggtat ctttaaaggc catattgata atatgtggaa
     9001 tttgctcttt gtttgcataa gctgccatat tttcttgcat attctgaatc aaaaacggat
     9061 ctaacaatga acctgatatt actgcttgat ggataccaaa tccaaaaaat agattagcaa
     9121 tagatgtaat taacaaaaat cctggtaaac cagttgtaat tttacttaat ggctctgtaa
     9181 ctatcgtggt aattagctta tttatatcca tattaaacaa ttgaatgatt ataaatgaca
     9241 ataaagcaaa cgaggtaatc gtaataatga ttgggattaa tacattaaaa gactttaaaa
     9301 cagcgggagg aatatttccg gatagctgaa tctgtagttt cttattatta gaaagtttaa
     9361 taaataattc tgtagccgct aatccaacaa atatacctac aaacattcct gaagctcctg
     9421 tataggatat aggtatcaca cccgtcactt caacagcttt atccgcattt tcaggagtaa
     9481 acatcatctg cataggtgtt aatacaacaa atgtcgaaat agcaactaat accgctgcaa
     9541 tatgatttgt ataatttctg cttgcacaca tatgataaga tactgctgct acaactaata
     9601 atgtagttat gcttaatgta ccattggcaa ttgaagtacc aatttgttgt aacgtggtca
     9661 atgtactagt atcgacaatt tcccccatga atcctgttgg ttctaagatc actgaattaa
     9721 taagtgtcac aaatcctgct aacatcataa atggcataaa tgtagcaaac gcatctcgca
     9781 aagttcttaa atggatctga ttccccaact tagtcgcaac tataccaaat ttttcaaata
     9841 caatgtcctt cctgctactt ttgcccatat tttttctcct ttatgcttat tgtaatcgat
     9901 tactttttcc acccaaataa tacatcactt atcaaaaaaa gtaaatactt ttaattaaat
     9961 atatatatct tttttagtat aaaaaaataa gctctgaaaa aataattttc ccgagcttat
    10021 ttttcaacaa aaacagcttc aaaaacaaag tcttgatata aatgtctcga taccgaatac
    10081 tcgaaaggtt taccagacga tagagcaaca actttgtcta tctcaatcat aaattcacct
    10141 ggatctattt ttaatatttt tgcgtcaaat tcattcgcag tatctccttt gatacgtatg
    10201 tgcgaagtct ttatttccag acccagttca tttgtgatat aagcatatac agatttcttt
    10261 aaatgttttg gttccaagcc tggtatgact gctaatggca tgaatgtttg ctcaatcgag
    10321 tatggttgac aatcaataaa acggcttcgt acaatattat atacatagtc ttttacactg
    10381 atacttaatt tatcagctac ttttttcgat ggaagttcaa ttgagaaagt atggatttct
    10441 gaatctacgt gttcttcacc aaccacgcta aacaaacctt cgtttggacc aagaactcgt
    10501 gagttatcaa ttggtgttct gacgacttca gtcccatacc cacttctact tcgcaaaact
    10561 ccttcggaaa caagtaagtc tacgcctttc tttactgtta acttgcttgt atgataaata
    10621 actgccaatt catctcctgt aggaagtttc tcaccaattt tatactctcc atttacaatt
    10681 tttttcttta tatctaagta aatttccata tacttcgcaa tttttttcaa tggtgaccct
    10741 ccaatcaaaa acatatcttc atctatactt taacataaat aaaccgataa aatccacatc
    10801 aaaattatac acacaataaa aaagatatat atatttaaac aaaactattt actttaagtt
    10861 gtaagcggtg ttatcattaa gtggaaacag gaggtaaaaa aattatgaca actatgaaaa
    10921 agctaacttt tccagatgat ttttggtggg gttccgcttg gtcagctgag caagcagaag
    10981 gaaaaggaga aacaggaaaa gcagagacta tctgggatcg ctggtttaaa gaacaacctt
    11041 accggtttta tcaacgaatc gattcctcta ttacaaccga tcacatccat caatataaag
    11101 aagatataga actaatgaaa aaaactgggc ataactcctt tcgcgtttct atttcttggg
    11161 cacgaatgtt tccaaatggc ggtaccggaa aagtaaatcc caaagccatt tctttctatc
    11221 gaaaattatt ttccgaaatg aataataacg gaatcaaagt atttgccaac ttatatcatt
    11281 ttgacatgcc tgctactctt caagataaag gaggatggga atcacgtgaa gtagttgatg
    11341 cttatgttca ttacgctgaa acttgtttta aagaatttgg tgacttagtt tgtcattggt
    11401 ttaccttcaa tgaaccactt gggcctattt taggtactta tcttgaagat tttcattatc
    11461 caaacctcat tgactttaaa cgcggagcgc aagcagcatt cttcactata cttgcacacg
    11521 caaaagcaat acaagtattt aaagaactag ctcttcctag taaaataggc gttattctaa
    11581 atttgagtcc tacttttcct cgtagtcaaa atcctgctga tgtgaaagca gcagaaatcg
    11641 ctgatttatt ttatacgcgg agctttttag atcctatggt taaagggaat ttttctaaaa
    11701 agcttatcct gttattacaa gaatataatc agcttcctga agattatacg aaagaagatc
    11761 ttgtatgtat ctacgaaaat accgctcaaa ttcttggact aaactattac gagccacgtc
    11821 gcgtcaaatc tcgttcttct atggtggata catctagtcc atttttacca gagtggtttt
    11881 tcgaaccaca tatcatgcct ggacgacgaa tgaacgaata ccggggatgg gaaatttacg
    11941 aaaaaggaat ttatgatttg tgcatggata tcaagaacca ttatggaaac atcgaagcat
    12001 ttatctcgga gaacggaatg ggcgttgcgg atgaagaacg tttcatggat gatacagggc
    12061 aaataattga tgattatcgg attgagtata tcaaagatca tcttgcttac ttatggaagg
    12121 caataaatga aggatgtaac attaaggggt atcatctttg gacatttatt gattgctggt
    12181 catggatcaa cgcctataaa aaccgatatg ggctggtttc actcgacctt actactcaaa
    12241 aaagaacaat taaaaaaagt ggcgaatttt acaagcaatt aagtgataca aatggattta
    12301 gatacgatgt agacttgtta gttgaataat attctaaaat tccacttgaa gtgaacacat
    12361 tagaaaacaa gaataatgag aaataccaat aacaaatact ttttcttcat acttcattta
    12421 tacttgtcag tataagagac tgtgacaaat gtagtgatcc tcagaagtta ggtctggaaa
    12481 tctaacttga gaagattttt ttaaactacg tccagtcttt tttatttgtt taaaattgaa
    12541 gttgacaagt cttcattaaa aatttagaat attcacaagt ttgacgacaa ttatgaaaat
    12601 ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa aactgcgatc
    12661 aaagacacgt tacctaccgt ttttggttat atcggtattg gacttgcatt tggtatcgtt
    12721 gggaaagctg ccgggtttca tccgctggtc gtcacgttga tgtccctgct gatctatgct
    12781 ggttctgccc aatttatcac ggtcagcatg cttgctagcc atagtccact gctctccatc
    12841 gttctctcga cctttctagt caattcccga atgattctga tgagtatgac gattgctcct
    12901 tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct gacagatgaa
    12961 agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa ctttcgttgg
    13021 atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct tgttggcgca
    13081 cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc catcgtcgct
    13141 atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc taaacgatta
    13201 cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat ttttatccca
    13261 agtaacttag tgattttagt tgtaacactg attgggtgcg gattgggggt gtggatcaaa
    13321 catgccttct tttgagttta cattgctgac tattttaggt tgtactttag caacttggct
    13381 ttctaggatc ctaccatttg tcttactgaa aaaattcgat ttgccgcaac cgttgcttga
    13441 gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca gtctatttac
    13501 ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt cagttcctac
    13561 acttttagct gctattcttt caaaaagctt attggtgatc gtcttggtag gaattctttc
    13621 cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa attactgtca
    13681 tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg
    13741 ttgaacggac cactaacaac gacttttcct aatatatgtc ctgtttctac gtctttggtt
    13801 gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa tgtactgcgg
    13861 ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg cgtttcgatt
    13921 tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat agattggttt
    13981 ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac atgtccaaat
    14041 ggtcggacaa ggtgtttgat ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca
    14101 tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg atgataatcg
    14161 attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact tgcagtcgcg
    14221 tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac gcccccagct
    14281 ccgttgatca ctaagatttt tttcccgcgg ttggcatttt cttctggagt cagaccaaat
    14341 ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc ggcttcttca
    14401 tctgaaagat tttttggtgc aagagcgaca atacgttcgt caaccagttg gtattcttga
    14461 ttgcttcctg ctcttgtcgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt
    14521 tgcacttcat cacctacagc aacaactttc ccgactccat caaaccctaa gatacgtaaa
    14581 gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg
    14641 ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata
    14701 aagctttttg gatcatcgat cgacaaccct tcataaaaac cgactacctt cattaattcc
    14761 ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat acagttttca
    14821 tttcaatata tatagtatgc tatttgttcc acttttattc atcataatta ttctcttact
    14881 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
    14941 aacctctttt aaacggtgtt caatcaactg accctcggta ttcgctcaaa aaacgacaaa
    15001 atatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac
    15061 gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt
    15121 ctttttcaaa ggtgtctaag atctctcgta cttcatcagt agatttcgta ttcatcaact
    15181 gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta
    15241 gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac
    15301 gaagtagtcc gatcagttct ttttctgtat gttccctcgg ttctttttca aatgcataag
    15361 gattcttgaa aatcccccgg ccaatcatga tgccatctac gccatatttt tcggccaatt
    15421 cttctcctgt ctggcgatct gggatatctc cgttgatcgt gatcagtgtt tgcggagcga
    15481 tttggtcacg taaagcaatg attttaggga tgacttccca atgtgcatct actttactca
    15541 tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac
    15601 tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag
    15661 gcaaaccgcc agctttagcc gcttcaatca attgtgccgc tacttctggg cgaaggatca
    15721 agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga
    15781 ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt
    15841 ctccccaaat atgggcaacc atcggctgtt catcttctgt aaaggtcaat cttcctcgta
    15901 cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga
    15961 catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca
    16021 ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt
    16081 tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta
    16141 tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa
    16201 aaaaagactg tgacaaaaat cttatcacaa ttccttttcc ggaataaatg gtgttcaaaa
    16261 gcttgcatct gcggtaataa gtccaaccaa acgaaaaata ggaggagcta tttttgtttg
    16321 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccacta aacggtgttc
    16381 aatcagctga ccctcggtat tagctcaaca aacgacaaaa tatgaggaac tattttcccg
    16441 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttc taacttctca
    16501 atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg atgaatagca
    16561 caagtacgat catcgatgtt gctactggca catcgattgt tccaaggaat ccgtaacgga
    16621 aaccggagat catataaacg atcggattga ctttcgaaac agcttgccaa aatggcggca
    16681 acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag acaaatgtag
    16741 gcacgatcga tacatcgtca taagactgag caaaaatccc gttgagcagt cctgctaacg
    16801 aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa tacacatgaa
    16861 gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata ctgcgaccca
    16921 ctccgccaat cacaaagccc caaatgatca catgcgtagg aacaggcgct accaagattt
    16981 cttcgatgtt tttctggaat ttttgagaga agaaggacga agaaacgttg gcatacgaac
    17041 tagtgatagc agacatcata atcaaaccag gcacgatgaa ttccatatat gaaaaaccac
    17101 ccatgtcacc gatccgacca ccgatcattt ttccgaaaat aacaaagtac aaggatgtcg
    17161 tgatcacagg cggaaccagt gtttggaccc agatacgcaa atagcggttc gtttccttga
    17221 tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc cgtatgatta
    17281 ttttcttctg tgattttcaa aaacagttct tctaaacgat tcgacttatt cctcatagaa
    17341 agaactttga ttccttgtct tgtcagctga tcgaagattc cgttcacccc ttgatttcgt
    17401 tcaacttcga cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc ttcaatcaca
    17461 ggtttggtct catatggcgc taaatcaaag ataaatgttt caaattgcag tttggataga
    17521 agctctttca tactagtatt ctcgatcagt tcacctgatt ggatgatccc gatgttgcga
    17581 caaagcattt cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt accattttcg
    17641 ttcaattctc ggagaaactc ccacatctcg cgtctcaatt caatatcgac tcctgctgtt
    17701 ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat catcagtctt
    17761 cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca aagattggac
    17821 tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc gtagtagcct
    17881 gcttgattca cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa ctcttgcggg
    17941 actaatccaa tctgttgctt tgctcggact agatccgtat ccagatcata accgaagaca
    18001 ttcacttttc cagatgtttt attgactaaa gaagtaatga ttccgatcgt tgtagacttc
    18061 ccagctccat ttggtcctaa tagcgcatag aaatctcctt ctttcacgga aagatcgatt
    18121 cctcgcaatg cttcgacccc acttccataa acctttttca aattatttat ttcaagcgca
    18181 tttgccattt atttcctatc tccttataga catgattctt tattgtatca tggaatacag
    18241 cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt gctgggctca ggaaaatctc
    18301 ctaatgaaag acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa caaatcgaag
    18361 ataaaggatt actattcata tgtgcaacaa aaggagcgtg agacaaaagt aaagaacact
    18421 tttgtctcac gctctaaaca cgtataaacg gcggaagcaa aagccaagat ttttgatcca
    18481 ataaatactt tcgatcactt cgcctatccc aatacatgcc actgccgctt ttcatcaaat
    18541 aacgcggctt ccgtttttgc ggtttctact aaagtctctg ggtaatccaa aactttcagc
    18601 aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc aaaactgatt
    18661 ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt ctttaaaatc
    18721 tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc cgcaagccac
    18781 cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgtcccttt taaaatctca
    18841 tctacgaagc agtagcagcg ttctttggaa gccacttggt ccaacaaacg tttgatcgat
    18901 ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat tgccatggaa
    18961 gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt
    19021 gctagaatac agctgatggc tatacttttg acatacgtag attttcctga tgcattggaa
    19081 cccgtgacta atgtattctt ctgccagttg accggattca ctaccgcttc ttttaacaat
    19141 ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg gcaagtgatg
    19201 ggcatataag tacggtaatt caatattgca gcggctactt ctaattctcc cagcaattcc
    19261 cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa atgataggag
    19321 ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaactctgc ttcactccca
    19381 ctcttcatac gaaatgaaaa acctaattta gggatgtctc ttaatggttt agtttgctgt
    19441 ttcagctctt cttgaagtgg tgtcggcagt tttgctagct gattggcggc tgaaatcgtt
    19501 gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt cactagataa
    19561 tagaccgtat tgaatactaa cccaccgatt gccaaataga ttcctagtac ttgtcctaaa
    19621 agaagcaaac ccaaacccac cagcggaagc aagccaagaa aaataaataa acctacgcgt
    19681 ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg atcttttttt
    19741 cctaaacgag cgaattgata ttggattttt tctctcagtt gaggattttc ctgataaaaa
    19801 gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa agctgcgtaa acgttgatac
    19861 aacgcttctg aacctacact agaataggta ctgttgattc cttcgaatac ttcgaacata
    19921 tccaggtcat accaagtaag atcatcgatt tcgctatccc atgaacggaa cttcttctcc
    19981 gtgagccatg cttccttcaa gctttcttct ttatcaaatc tcggttgata aggaattttt
    20041 tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg aaaaaatgtc taaagcaact
    20101 aataaaataa cagctgcaat aataattaaa acgataaatt ctctttgacc catatccctc
    20161 tccgttcctt tatttgttag ctttagtata gcactgttgc gctatttgaa aagagggtaa
    20221 aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat attacatatt tatctctcat
    20281 ttatcaaaat tgacaaataa tcatagggag gaattgttgt taattcttgt ttattctcca
    20341 gtgcattgac aactgtccca agggaaagga tagcctcagg tactattttt tcttgttctg
    20401 ttggattagc taagcgaaga acaaatcctt gttcatgaaa ataagaagga taaatcgatg
    20461 atacaattaa gtcttcaggt aattcaatca atgaaaattt acgaggaaca tcaagttcat
    20521 tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa tacattcagt ttctgctttt
    20581 gataagaaat agattgttct aaccgttttt ctgcctgttt tgccactttg aactcattaa
    20641 atcttccttc ttctacatta aatccaaaag tgactttata ctcacctatc tcttgagcta
    20701 gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc tcctgaagct cgcccaggtc
    20761 gccaagcaag attaggtttt ccaagttccc ctgttgttga aaataaggtt atgattaatt
    20821 gattttctat acgttcatac tcttttaaac catctgcata aaacgtaaaa taatgatcat
    20881 tttctgccac agagacactc ttatcgaata tttctaaatt aacaggtttc tctaccatag
    20941 tttctgacca attcggagga atgacatcag gggtattttc aataaatccg ttttgaattt
    21001 gtgcaattga tttttcagat ttatctaaaa cattaattgc taagcgcata cgatgcgagt
    21061 aaacagtatt atgaattttg agtgtgcctt caacgatttg actttctccc agactgaacg
    21121 atatttcata agatatttct tccatttcgg gtttttcaga taaacgatct tttaaatctt
    21181 taggaagttg agcttttccg taaacaacta atgtttcttg taaagaatct ttatatactt
    21241 tagctgtttc taattttaga gatagttctg tatctccttc taatggtgaa taatcatacg
    21301 tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc aagatattgt ctatctccaa
    21361 ctatcaaatt caattgccca tctttaaaag aaagtttata tttattgtta cttatctcag
    21421 ttttgtctat ctcgattgct gtttctaatt tttcattatt tttctcaaat tcaacaacag
    21481 tgtatcctag tgctggcaca tttacattta tttttatgtc aagttcataa taagctggtt
    21541 catcaaaata ttcgaaacct gtagcaatca tccgataagc atttttccgt tctggaaaat
    21601 aaacttcttt ttcaatgata ttatagggac tacctttgaa gtgtattttc tttgaatcag
    21661 ctactacatg cacgattttc gatccactat aattctcagt cgttgtgttg aaaacaagga
    21721 cttcattcga ttttagcttt aatgcatcag ccatccgttt tacaataagg ttttcgattc
    21781 cgtcagctat ctcattagct tctttgattc gatggaaaat atcttccgca acattatctg
    21841 aaacacagcc accaatacta tcatgagcct ggttttgcaa aatctttttc cataaccgac
    21901 gaagtaggcc tttactgata tggataccat ttctttctgc aatcagcatg agcggctcaa
    21961 ctcgttttag aattttttgt tccaatttaa aattttctat tttcattttt gttcttactg
    22021 agccaataga acgatgaaca cgagagtaaa ccggctgacg aagttcacct ttatattcag
    22081 gtaattcttt tttgtttctg ataacttcta cataagaagg ataatctgaa ataatattgt
    22141 ggaatttact gtatttattc aacttctcta cgacttgatc aaaatttaaa atcattgact
    22201 tttgatctac acccgaaggt actaaaatat ctttgttttt tccttgcata tgaagcattt
    22261 caactgcttc atccaacttt tcttttgcaa attcaggaat atctttaatt gcgtctaagg
    22321 tcatcattcc agtcatataa ccaaatggaa aattcatggc ataaacaaat ttatctccca
    22381 atcctttcca gataaaataa ggagatggaa ccatcgtttc aaagtttaat cctcgccaac
    22441 acatgaaatt atcgatttct acttgatgca aaagtgtagg caaattcgca ttgaacccaa
    22501 acgtgtcagg caaataccct accatcattg gatctccata tttaaggatc gtatcactga
    22561 ttccaatcat taagtttctt aaaattgatt cagcatccac taataaggca tctgtttgtg
    22621 cataccacgg tcccacgaat agttgtcctg ttttcatcag tcgttggata accttaagct
    22681 tttgcggatt gatctccaaa tactcatcga cgattgaagt ttgtccatct aaacaaaagt
    22741 ttactttagg gttcctttcc aattcatcta gaacttcagt aaagacctga tcagacaata
    22801 cgatggcatc ttcatttgta aaataccatt ctctgtccca gtgcgtgtgt tgaattatat
    22861 gtgcatttac catatcagat tctcctactt tccttcttct actgaattta cttgttgttc
    22921 ttttttatac ttgatggatt ttagtgcatt gaccattaac gctgtaacca ctcccccaat
    22981 aacaattgat actaaatata aaggtatatt cgtaacaaaa ggaattgcca taaaggttga
    23041 aagcattgtt tcttgagtaa caccaaaaaa tccatttaga ccacatgcta ctgcagtgcc
    23101 caccacgatg cttggtatta ctcgtaaagg atctttcacg gcaaacggga tcgtcccttc
    23161 agtaatcccc acaaggccca taatcatagc tgtctttgca ttatctcgtt ccgattcatc
    23221 ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca aatggcggaa tagcaattcc
    23281 taataacatg gctgtactag gtccaaagct tccgcttgaa aaagaagcaa ccccaaaagc
    23341 atatgcgatt ttattgatag gtcctcccat atcaaacgcc atcattcctc ctaaaatagc
    23401 cgacatcaaa gcagcatttg ctcctgacat gccttctagc caattagtca gtcctgttgt
    23461 cattgcttga ataggcgtag ctagtacata ccataatatg attccaacaa ctaatgtagt
    23521 aacaaaagga atcagcataa ttgattttag actaataact tgcatcggta cttttacttt
    23581 tttcaatgca ttagctaaat agccacaagc taatccagca accattcctc caataaatcc
    23641 cataccaaga ttttgacaca tcatcccagt gataaaagct ggtgctagac cgggacggtc
    23701 agcaattgaa tatgcgatat aggctgaaat catcggtacg ataaactgta atcccgtctg
    23761 accaatcatt cgcaggactc cccagaatgt atccgtctgg tcccaaacac ctgtgccacc
    23821 aaaaatgact gcaatagaag ttagtaaccc tgctgctacg acaagcggga tcatatagga
    23881 aaccccactt tggaaatgat ttagaatttc tcttccttgt ttcttgaatt ccattagtaa
    23941 ctcccccacg atatattttt tatttgttaa ttaatgcaat tgcttcatca atcgtctctt
    24001 caccatcttt tactacctct gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac
    24061 ctcgttctga aacatttgta tctactgcaa atatgacaac atctgcctcg gaaacatccg
    24121 aaattgaaag tttgttttct atccccatag ccccctgtgt ttctacttta atatctaacc
    24181 ctttcttctt cgcaaacttt tctaaattag cttttgccat ataagtatgg gcaatccctg
    24241 cgatacatgc tgttactgct actatttttg tcatatgaaa ttcctccttt taaaatgata
    24301 gagcttttac caattctttt ttatttctcg aatcttttat tcttttaaca aactcgtcat
    24361 ccatcaatct tccagataat tctgctaaaa tacgtaaatg attagtacct ttatcagtgt
    24421 cttttattgc taacaaaaag acgagatcaa ctggttgatc atctaatgcg ccccattcaa
    24481 cagaccgctt taacttggct acagccactg ttgattcaat tactgctagg cttttgccat
    24541 gaggaatggc gatatttttc ccaattcctg tggtcatatg gttttctctg tcaaatacgt
    24601 ctttttgaaa ttgtttgtgg tcagacacaa acccattagt ctctaacaat ttcgataatt
    24661 tttcgactac acttttttta tcagatgcat ttaaatctat cactatatta tttatattga
    24721 ttttttcact gatcatttat aagccttctt tcaatatata aactaatttt tcaaacgacg
    24781 aactcgtttt taaatttgct aataactcat gattcgaagc gatttcattg aatgtttcat
    24841 aaattttatt caattcaggt tttacttttt cattaagtgc aaaaaagaaa acagaatgaa
    24901 caagtgtccc atcccaatca attccttctg gattaacgta gacacagatt actggctttt
    24961 ttataaaatc tacttcgata tgcggcatag caaaatcatt catcgctgtt gaagatagtt
    25021 tttctctttt gattgctccc tctagtatcc cttctttcgc gtaattttct tttaccaatt
    25081 ttttggtaat tatatttaaa acgttcactt gattatcctt tatttggtgg gtaaaaaagc
    25141 aaaatcgttt atctaataaa tttagaaaag catcttctgt ttgttcattt aactgtagat
    25201 actgttttac ttttctgaca tctgccccta acatcaaagg actaacaaca ataacaggaa
    25261 aatttgtatt ttcaatcgga atcgtgctta taacaagttc atcagtgact tttctatctt
    25321 gtagttcatt gatgcttagt acatcttcta tctcaaccat atctgcaaaa ccgttattta
    25381 ttctttgctc taataatttt gatgttccat atccgctact acaaactaat atcactttcg
    25441 ttttgtcagg tttgtaacga tccaaaagtg attgaatatg gagtgttacg taagcgattt
    25501 catcttcatt aaaatgtata cagaatctct cttcgatttc ctctaataaa tctacactta
    25561 tcataaatga ccttttaaaa gaatatttga ttttgtccgt ataagggtta tagatattta
    25621 ctcccaattt gagtctttta actgctgcat tgagatgtaa tactagatta ttgatcaatt
    25681 cttcatcagg ataaaattca tttaatttaa ttcttactaa ctcttgtagc tcaacaccca
    25741 tatctaaata ggaaagcttc gtttgatcta agtcgtgatt catagcattt ttttcaatcg
    25801 aagtaatgtg aatgtcaaca taagcttgtt catctattgg cacaataatt gaaaatgctt
    25861 tttcaatcat tttgattaaa ataagtgtat tctttgagat ttcttcttca gaattattac
    25921 tacgtgataa gaaattgctt gtttcaatcc tttctatcgc aatagcaagg tggatagcta
    25981 aagtttgaaa ttcataatct gtaaatttca attgtgtctt attcatgtat cgattaagca
    26041 attcggctac ttttttcaat atgtccttat ttaagaaatt attcgcttca ccaatgaaat
    26101 tgatattcaa gtctaagaga tctgttttac tgttagatgc tgtaaagcca ccccaataat
    26161 agttcacaac ttctgacatc aaccttcttt tacttttttc atcagctttg atgcgcacgc
    26221 catttcgatc tgaaactaag gacatcccat gcttgtttag tctttttcta acatctttca
    26281 ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc agcaagattt tgaatcttta
    26341 gagaatcatt agtattcaat aatttagcaa acacataagt tagacggtcc tccggagagt
    26401 ctgcgatatt gattttatga ctggataata aatttcgaag tgtttctttt tttccaataa
    26461 gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc tggcattttt tggattattt
    26521 ctttcaaaat taaaatatct ttgctaattg ttttcgtaga tacatataat tgttctgata
    26581 attctttgta agttgtgtgc tcattttcaa tcaaaaagta tagtattttc atttgccgtt
    26641 cgctaaattc gatgcccatt atcttcacct cctgaataca gcttaagtgt acaattatta
    26701 tctctatttt catacag
//
LOCUS       DAZKCE010000028        26666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000028 DAZKCE010000000
VERSION     DAZKCE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.028"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8KQ_001061"
     CDS             complement(104..1336)
                     /locus_tag="ACG8KQ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9104009.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8KQ_001062"
     CDS             1591..2160
                     /locus_tag="ACG8KQ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9104010.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8KQ_001063"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8KQ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9104011.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8KQ_001064"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8KQ_001064"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9104012.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8KQ_001065"
     CDS             3732..4655
                     /locus_tag="ACG8KQ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9104013.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8KQ_001066"
     CDS             4731..5870
                     /locus_tag="ACG8KQ_001066"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9104014.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8KQ_001067"
     CDS             5863..6663
                     /locus_tag="ACG8KQ_001067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9104015.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8KQ_001068"
     CDS             6663..7490
                     /locus_tag="ACG8KQ_001068"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9104016.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8KQ_001069"
     CDS             7495..8202
                     /locus_tag="ACG8KQ_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9104017.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8KQ_001070"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8KQ_001070"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9104018.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8KQ_001071"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8KQ_001071"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9104019.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8KQ_001072"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8KQ_001072"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9104020.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8KQ_001073"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8KQ_001073"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9104021.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8KQ_001074"
     CDS             11787..13820
                     /locus_tag="ACG8KQ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104022.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8KQ_001075"
     CDS             13864..15144
                     /locus_tag="ACG8KQ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9104023.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8KQ_001076"
     CDS             15354..16160
                     /locus_tag="ACG8KQ_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104024.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8KQ_001077"
     CDS             16172..17392
                     /locus_tag="ACG8KQ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104025.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8KQ_001078"
     CDS             17382..18968
                     /locus_tag="ACG8KQ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9104026.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8KQ_001079"
     CDS             19007..21145
                     /locus_tag="ACG8KQ_001079"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9104027.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGALLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8KQ_001080"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8KQ_001080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9104028.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8KQ_001081"
     CDS             22874..24265
                     /locus_tag="ACG8KQ_001081"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9104029.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8KQ_001082"
     CDS             24281..25321
                     /locus_tag="ACG8KQ_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9104030.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG8KQ_001083"
     CDS             25340..26425
                     /locus_tag="ACG8KQ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9104031.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26666
                     /locus_tag="ACG8KQ_001084"
     CDS             26458..>26666
                     /locus_tag="ACG8KQ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFP9104032.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFS"
BASE COUNT         8539 a         4441 c         5421 g         8265 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gcattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccat
//
LOCUS       DAZKCE010000029        26064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000029 DAZKCE010000000
VERSION     DAZKCE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.029"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8KQ_001085"
     CDS             <1..1125
                     /locus_tag="ACG8KQ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9104033.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG8KQ_001086"
     CDS             1474..2106
                     /locus_tag="ACG8KQ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP9104034.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="ACG8KQ_001087"
     CDS             2694..4133
                     /locus_tag="ACG8KQ_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9104035.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="ACG8KQ_001088"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="ACG8KQ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            4895..5110
                     /locus_tag="ACG8KQ_001089"
     CDS             4895..5110
                     /locus_tag="ACG8KQ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104036.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="ACG8KQ_001090"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="ACG8KQ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="ACG8KQ_001091"
     CDS             complement(5770..6681)
                     /locus_tag="ACG8KQ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9104037.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="ACG8KQ_001092"
     CDS             complement(6700..7704)
                     /locus_tag="ACG8KQ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9104038.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="ACG8KQ_001093"
     CDS             complement(7701..9824)
                     /locus_tag="ACG8KQ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9104039.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="ACG8KQ_001094"
     CDS             complement(9829..12276)
                     /locus_tag="ACG8KQ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9104040.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="ACG8KQ_001095"
     CDS             complement(12263..12652)
                     /locus_tag="ACG8KQ_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9104041.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="ACG8KQ_001096"
     CDS             complement(12712..13215)
                     /locus_tag="ACG8KQ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9104042.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="ACG8KQ_001097"
     CDS             complement(13228..13452)
                     /locus_tag="ACG8KQ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104043.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_001098"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_001098"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9104044.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16349)
                     /locus_tag="ACG8KQ_001099"
     CDS             complement(16212..16349)
                     /locus_tag="ACG8KQ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9104045.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG8KQ_001100"
     CDS             complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG8KQ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9104046.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="ACG8KQ_001101"
     CDS             complement(17789..18079)
                     /locus_tag="ACG8KQ_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104047.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG8KQ_001102"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG8KQ_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19287..19451)
                     /locus_tag="ACG8KQ_001103"
     CDS             complement(19287..19451)
                     /locus_tag="ACG8KQ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104048.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19444..20118)
                     /locus_tag="ACG8KQ_001104"
     CDS             complement(19444..20118)
                     /locus_tag="ACG8KQ_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9104049.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="ACG8KQ_001105"
     CDS             complement(20196..21536)
                     /locus_tag="ACG8KQ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9104050.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="ACG8KQ_001106"
     CDS             complement(21619..21954)
                     /locus_tag="ACG8KQ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP9104051.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="ACG8KQ_001107"
     CDS             complement(21964..22338)
                     /locus_tag="ACG8KQ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9104052.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="ACG8KQ_001108"
     CDS             complement(22351..22665)
                     /locus_tag="ACG8KQ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9104053.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="ACG8KQ_001109"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="ACG8KQ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9104054.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7500 a         5452 c         4557 g         8555 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aacagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacagg ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttg
//
LOCUS       DAZKCE010000030        25763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000030 DAZKCE010000000
VERSION     DAZKCE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.030"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACG8KQ_001110"
     CDS             complement(183..1502)
                     /locus_tag="ACG8KQ_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFP9104055.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(1658..1990)
                     /locus_tag="ACG8KQ_001111"
     CDS             complement(1658..1990)
                     /locus_tag="ACG8KQ_001111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104056.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(2004..2261)
                     /locus_tag="ACG8KQ_001112"
     CDS             complement(2004..2261)
                     /locus_tag="ACG8KQ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104057.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2456..2998)
                     /locus_tag="ACG8KQ_001113"
     CDS             complement(2456..2998)
                     /locus_tag="ACG8KQ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFP9104058.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(3019..4110)
                     /locus_tag="ACG8KQ_001114"
     CDS             complement(3019..4110)
                     /locus_tag="ACG8KQ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9104059.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(4118..6328)
                     /locus_tag="ACG8KQ_001115"
     CDS             complement(4118..6328)
                     /locus_tag="ACG8KQ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9104060.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(6344..8872)
                     /locus_tag="ACG8KQ_001116"
     CDS             complement(6344..8872)
                     /locus_tag="ACG8KQ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9104061.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8924..9334)
                     /locus_tag="ACG8KQ_001117"
     CDS             complement(8924..9334)
                     /locus_tag="ACG8KQ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP9104062.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(9347..9565)
                     /locus_tag="ACG8KQ_001118"
     CDS             complement(9347..9565)
                     /locus_tag="ACG8KQ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFP9104063.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9562..10554)
                     /locus_tag="ACG8KQ_001119"
     CDS             complement(9562..10554)
                     /locus_tag="ACG8KQ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9104064.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10633..11544)
                     /locus_tag="ACG8KQ_001120"
     CDS             complement(10633..11544)
                     /locus_tag="ACG8KQ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9104065.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11563..11907)
                     /locus_tag="ACG8KQ_001121"
     CDS             complement(11563..11907)
                     /locus_tag="ACG8KQ_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104066.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11927..12205)
                     /locus_tag="ACG8KQ_001122"
     CDS             complement(11927..12205)
                     /locus_tag="ACG8KQ_001122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104067.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(12564..13064)
                     /locus_tag="ACG8KQ_001123"
     CDS             complement(12564..13064)
                     /locus_tag="ACG8KQ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104068.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(13065..13610)
                     /locus_tag="ACG8KQ_001124"
     CDS             complement(13065..13610)
                     /locus_tag="ACG8KQ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104069.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13607..14863)
                     /locus_tag="ACG8KQ_001125"
     CDS             complement(13607..14863)
                     /locus_tag="ACG8KQ_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9104070.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(15224..15535)
                     /locus_tag="ACG8KQ_001126"
     CDS             complement(15224..15535)
                     /locus_tag="ACG8KQ_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104071.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15558..17033)
                     /locus_tag="ACG8KQ_001127"
     CDS             complement(15558..17033)
                     /locus_tag="ACG8KQ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFP9104072.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(17482..17934)
                     /locus_tag="ACG8KQ_001128"
     CDS             complement(17482..17934)
                     /locus_tag="ACG8KQ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104073.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17936..18301)
                     /locus_tag="ACG8KQ_001129"
     CDS             complement(17936..18301)
                     /locus_tag="ACG8KQ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104074.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(18400..22683)
                     /locus_tag="ACG8KQ_001130"
     CDS             complement(18400..22683)
                     /locus_tag="ACG8KQ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFP9104075.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22830..23069)
                     /locus_tag="ACG8KQ_001131"
     CDS             complement(22830..23069)
                     /locus_tag="ACG8KQ_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104076.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(23159..23401)
                     /locus_tag="ACG8KQ_001132"
                     /pseudo
     CDS             complement(23159..23401)
                     /locus_tag="ACG8KQ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23514..23765)
                     /locus_tag="ACG8KQ_001133"
     CDS             complement(23514..23765)
                     /locus_tag="ACG8KQ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104077.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            23928..24887
                     /locus_tag="ACG8KQ_001134"
     CDS             23928..24887
                     /locus_tag="ACG8KQ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9104078.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT         7637 a         5485 c         3935 g         8706 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaattaaa agtatgaatt attcaggtta tgaattcatt ccccaaaggc
     1681 cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatac aagtctttta
     1741 aatgatccca tatcagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac
     1801 ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg
     1861 catcttggat ttcaataata gtatcatcaa ttagtttttg aatttcttgc ctatcaacat
     1921 attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct
     1981 cttcagacat tttcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt
     2041 gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc
     2101 agatttgtcg gtccatacgg atagcctttt tgacattcaa tgttcggatt ataccagata
     2161 ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca
     2221 aatccaatac acatcacact atagccaatt agttttttca tctcgtatcc atctcctaaa
     2281 aaatagaata gttcttatag ttattcataa gacatatttt ttccgttttt gttttatcct
     2341 ttctgtccta taacaacttt tttcaagtaa agaatgacat agtttttctt aaaaaaacag
     2401 tctgtttttt tctatttctt cggtgtataa taaatatgag tgttcatttt ttattttaat
     2461 acgcactccg tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt
     2521 tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat
     2581 attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt
     2641 gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc
     2701 tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt
     2761 ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg
     2821 aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt
     2881 cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac
     2941 aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt
     3001 ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt
     3061 gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga
     3121 gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg
     3181 agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt
     3241 tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat
     3301 cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc
     3361 cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt
     3421 cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca
     3481 atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc
     3541 tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag
     3601 aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct
     3661 cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa
     3721 accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa
     3781 ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga
     3841 ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc
     3901 aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat
     3961 cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc
     4021 atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa
     4081 aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt
     4141 tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg
     4201 gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag
     4261 aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt
     4321 gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag
     4381 agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca
     4441 cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc
     4501 cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc
     4561 tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc
     4621 ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg
     4681 ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac
     4741 tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc
     4801 cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg
     4861 cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg
     4921 ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat
     4981 gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct
     5041 ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat
     5101 ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca
     5161 aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg
     5221 actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa
     5281 aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa
     5341 tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg
     5401 ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat
     5461 cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt
     5521 ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg
     5581 tcccataatt taacaatata tacggatcaa caacagccgc tttataataa agttggcgca
     5641 ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg
     5701 ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat
     5761 catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc
     5821 aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt
     5881 tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac
     5941 caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta
     6001 actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa
     6061 tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg
     6121 ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat
     6181 caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca
     6241 ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt
     6301 tcttttcctt atgctttata taagccattg tttcacctca tttctattgc atgattaacg
     6361 tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca
     6421 tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc
     6481 aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac
     6541 gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa
     6601 aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt
     6661 gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg
     6721 aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt
     6781 ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg
     6841 catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta
     6901 aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac
     6961 gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt
     7021 gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt
     7081 tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac
     7141 ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct actcgggata
     7201 aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat
     7261 aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt
     7321 gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta
     7381 cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg
     7441 tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa
     7501 tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg
     7561 ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg
     7621 attccatacc taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg
     7681 ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca
     7741 attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa
     7801 ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga
     7861 tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc
     7921 gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg
     7981 ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc
     8041 gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa
     8101 atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac
     8161 tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat
     8221 acgttccttg attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca
     8281 ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa
     8341 tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca
     8401 aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta
     8461 accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga
     8521 ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt
     8581 cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt
     8641 caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat
     8701 ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg
     8761 tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat
     8821 ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc
     8881 acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa
     8941 catacttatt cacaaaaagt aattcttcca atgcattgtt tgataccttg tcccagttat
     9001 aatacacaca atcaccattc atcatacgaa tcatataaca aaaccaatcc cataaataaa
     9061 aatgcacttt ttttccatca ggttttagtt ttgccagtgc taacacacat aaatagggga
     9121 gtcctatata aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg
     9181 aacgtaaaaa taaatattca aagattacga ccaacaaaaa agttgctgca ttgactaatg
     9241 caactggaaa tggaagacgc caattgcttg taatctgttg tacgcgataa ggttccgcat
     9301 aaacaatcga ataatcataa ggttcccaat ccacttcact atcctcctaa tccataaata
     9361 atttttcaat gattttacca aacgcatcaa atacacgtgt tggatcattt aagaagaaat
     9421 aaactgcggc acctgctgcg atagatatta atgcatttcc tacttttgat ttcataaaat
     9481 ttcgcaatcc cagataaaca accaccatta ttaaaaaata gccaccttgc gttaatataa
     9541 aatctacaat tcccttcagt gtcatttccg ttcttctcct ttcctatcca accaattatg
     9601 tgttaatttt tcaacataat actgatttgt ttttttcgta atgacaatcg tcatttgttc
     9661 tttttgtaaa agtgacgtat caccatctcg aaagacgacc gttgctttta caataaaagg
     9721 ttttttcgtt tctttataac gcaatacttt catctctact tgatcaactt tagctgtttg
     9781 attgagtcct tcaggttctt tcatcatgta tgacatatct tttgcattgt ttttcgcata
     9841 cttatctaag aaatcttcca caaatccttc aacatctttc gcttccgacg catcaaccgt
     9901 ttcgtataat gtgtcatcat aagtaatcat tcgttgtttt tctgtattct ttggtagtgc
     9961 cgtaaaataa ggataagcaa taatacgaca gcccttgttt gaaatagaaa agggaagatt
    10021 gagtacttgt gtgattgtca cacttgtttc tttcttactc tctggattca tcgagcgaat
    10081 atcataagtt actttataac tagcagttgg aatggtttca atttctttta atccaataaa
    10141 cgaagcagca gttaattttc gaacacctga aacacttcct ggttcttgaa taaagttttg
    10201 tgcataaaac tcttttttta atgtttcttg acgttgttgt tcttgttcat gattatcgaa
    10261 tgtattcata tacaaagcaa caaaacgatt cataaaggat tcaatttcag gtgtgtacgt
    10321 taattctgac cgactttgta cttctttctt ttgtaattgt tggatttcat ctttcaaaaa
    10381 cgacacttga accttcgttt tcatggattg aaaaaatgcc aagccccctg aaaaacaaag
    10441 aaataaaacc aaacaccaaa tccatttcac tgcttttttt gaatggattt tcttttgctt
    10501 cggtactttc tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt tcataagcta
    10561 ctccttctag tatgttttta ctagtacgta cgattgacaa cgataagctt gtttttctga
    10621 aaaatagtcc attcaattac cctcattttc tattcctttt ctctttcttt tatatccaac
    10681 aaatccagca atacttgtca acactcctcc tagaacagaa agtgcaacat gtatttcttc
    10741 accagtcttt ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa tattgccact
    10801 tggaagtttc tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac tatcttggat
    10861 taatggaata ccatctttta caccaacaat tgtttgatca cctgctactt ttaatggggt
    10921 ggttggtgtt ggaatatggg tcaattcctc tacattacta gtgacaatcg gtacactcat
    10981 tccttgagaa gtattcgtcg taatttcacc gcttggggtt accgatactg tgacttcttt
    11041 tgctggaaca ataggtttat tttcttttgg aattgcttct gatttttcaa caggttgttt
    11101 cggttcttct ttttgttctt cactggtaga cgacatcgtt ggctgttccg tacttgacga
    11161 atcagtattc ttttcagaag aactggttgt tcctgattgt tccggagttg tgactatact
    11221 tgaatccgtt gttgttgaac tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt
    11281 tgattcagga aactctggtt ctggtgtcac tggctctttt tcaggtttct ctgactgcaa
    11341 ttgttcaggt tctgctggtt tttccgttgt tcctccattt agctctgtct caacatctgg
    11401 taatccttcc cctggcaatg tttctacaat ttctgactta ggtgtttcta cagtttcatc
    11461 tgcataaacc aatgaactcc ctaattgaaa cgaaaataat gaaattccta gtgttgttac
    11521 gactgctaat gttgtttttt tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt
    11581 gttcatagac gccgcactaa tattatctga acgataaaag aactcccgtc cgttttcttc
    11641 taacccaaca tatggattat ttttgaataa ttgaatcaat agttcactgg tgttgtattt
    11701 tggatagttt gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa
    11761 ttcataacga taccgcttca atccttttcg ttgttttaaa ggatctctat accaacttgt
    11821 ccatcgttcc attcgtggat agagatagac acgatacaca tagaacaatg ccataccaca
    11881 taccaccaaa aataataatc gccacataaa aattccccct tttcttctac ctttcttgaa
    11941 tcatcgaaca ttcataccct ctatagagat ttgaaaaatc catcatcaat tgttcatata
    12001 aacgttgttg caaatagcga ttgtttcgaa tacataattc ttcatagcca tcaatcactt
    12061 gatcttcttt ttcatagcat cttgtagctc tatgacgctc agcttgcatg acataaccaa
    12121 actctgcacc atatcgttct actgttaata actgttgact acgcaataat gcaatataac
    12181 aatcacaaaa atgttcaacc gacatttgat ttttctcacg tttgtgaaat aggtattctt
    12241 tagaaagcac attattcgtt aaacaatctg ataaatagcg ataatctggc ggtcggttat
    12301 tttcatctgt gaatttttcc cataataaac tgatttttat tcgttcagcg gctagatagt
    12361 cttgttcgcg tggataaaat tcatcaatcg cacaaagttt tttatcacct gaataaacaa
    12421 atccatataa tgcgaaacga actcttcctt ttcaattggc tctcctaaaa attccagtga
    12481 cataccatct tctccttcct aaaaaaaata aaaaagcaga aaccgatttg gcttctactt
    12541 ttctttcctt ttatagctct tttttacgga agaatccgca aatgacattt tttcaaaaaa
    12601 tcaatcggca caagttcacg aaaacttgga gcaattttgg atagaatcac ttccccatct
    12661 tgtaaaatac aatcataaaa atcatcagtt acttgatact tacgcttctt acaataattg
    12721 ataaaatagt agcgatcatt ttctatttcc attcgttgtt ctactaagcg catacgaaca
    12781 tcatcataga aataagccac aaatcgccct tcaagttcat gtttttcaat gggatactct
    12841 tctggtaaca atctattttt tcgtcgttta tacattgctg gataccttag aagaagttca
    12901 tctaaacgtc tgtcagtata tggtaactga cgaatcaacg attctaatcg ttctttatac
    12961 agatagaata attggcgcaa gcaatagtgt accgcctctg gattttctaa tactgttgga
    13021 tcacgtgacc aatacgcacc aacataaatc ataatttttt tcatttagac aatcacacca
    13081 gcaatactaa tgaccgatcc acaataggta gaacaagtgg catagcgacc aatttcctct
    13141 aattctatct caatctcttc taaagaaaga tatgtctgtt cttctgttgg ctggcgactc
    13201 atttcccaag tgacagtcac caatgaaatt tcttccataa gaggtttttc gctgctaagt
    13261 gctgccacac agttttcata tatcttcatt ttatcgcagc cttttttaac cgctaaaatc
    13321 gcaggtattg gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc tttccttcgg
    13381 ctaaattcca actcttcgag ttcttgttcg tatcccttac gaacttctaa taagcgtgca
    13441 atttcttgcc agttctcttt ttcctgataa cgtccagctt ttaccgcttg ttcaatataa
    13501 ggaactcgaa aacgaaaata agacaaatca accatctttt catttaatgc ttcgattgca
    13561 gcctttaaat ccaacaggcg cagccattct gttgtcgtat atgcattcat gcaattttcc
    13621 ctcctgacgt tcccatccat tctagatagt tttgttgctc gtcatttagt tcggcattat
    13681 caatcccttc ttgtacaaag tctcttccta aaatacgatc tgcttcttgt aacaacttta
    13741 atgtcggagc tacttgtttc aaaaaccagc gttctgaacg atctaatgag aatttttctg
    13801 gctttactgt aaaatccaat tcttccatcg aacgaaataa ttttgcccat cgtttatcaa
    13861 caatccgtgc atcactttcc agtctatccc aaacaataag ttttgcatta attaaaccaa
    13921 cgccaatctc tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct actgcttgtg
    13981 ttgctttctc attggctaac cgtaactcat atcgattata aataccatat ttcgttaatg
    14041 cttctaattc tgaaattttt tctcggttcg caatttcaaa acgtttctca taaaaattaa
    14101 aaaagagagg actctttcgc gatccaaaat acaaagataa tccttcactt cgaaaatagc
    14161 catcatctaa aaaacaaccg ccaatattct ggaatctctt aaagtccatt tctaataaac
    14221 ctgctttttg tttttctagt aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc
    14281 aaagttcatc taacgcaata tcgaatcggc tacattgcac acgtgtataa ccagcttcca
    14341 actttccttg atttttccaa atggaatata gccatgttcg ccaatctgtt tgttgttcac
    14401 gcatgacttc ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt acattttctg
    14461 ttacagttcg ttttgaatgc cagaacaccc agatattccc acgaacagaa agacagtcaa
    14521 aaagatacaa gcccgttgga cgtgtttcaa attctgtcag atccattcgt aatacacgtt
    14581 gaatcactgt tttgtagtct gtcccaaaaa aggaaagacg caaataatca atcattgctc
    14641 gaagatctat tccgcttttc atatacaatc gaaagcgttc cttgattcgt tgttcaaaat
    14701 cctctgggat ttttcttttc ccttgttcca tctttctaat gtaatctttt gagcagccca
    14761 tcaattgcgc aaatcttgct tgtgatagcg gctttttatc tccacttata ttatgaattg
    14821 cacttaactt ctttctgtag ctttttagct caatcggtgt cattttctcg ctccttttat
    14881 aaaataacaa aatcgatttt ttttaaccct ttagaaacac cgatatatca actagtcccc
    14941 ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg atacaaaagg
    15001 gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta tatatcgggg
    15061 ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc
    15121 tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc caggcagagt
    15181 gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa attaaatgat
    15241 ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt gacaaagtta
    15301 aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct atatcttttt
    15361 tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc
    15421 ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg gaagaaagag
    15481 aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct gccatttaac
    15541 ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc ttttggatct
    15601 tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc atctcccatc
    15661 gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc agtaaatttc
    15721 tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt
    15781 acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta aaatggctta
    15841 tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg accaacagaa
    15901 atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc tggtcgttgc
    15961 attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg ctgaatcaat
    16021 ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc ccattcatca
    16081 aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg ttcatattta
    16141 ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa tgcatttcgt
    16201 aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa tacatcgacg
    16261 tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt cccaacactt
    16321 aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat tgcaccagca
    16381 atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa atgaatacgt
    16441 ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag gaatacataa
    16501 ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcagataac
    16561 aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg aaaagaaacc
    16621 actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt ttcccttgtt
    16681 tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa cattcgaaca
    16741 agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc
    16801 aaccctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat tccaggaata
    16861 agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt ccaactaaac
    16921 caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa ccataaataa
    16981 cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt caattgtcac
    17041 attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa agcaactaat
    17101 acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg atttttataa
    17161 gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc ctgatgatct
    17221 tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca
    17281 ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc tccaaaatac
    17341 tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt tgattggttt
    17401 tatctgaatt caaaatctcc ccttccttct atcttagata aaaagaatag gaagactcct
    17461 tcctattctt tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa cacttggttg
    17521 tttttgatct aaaccttttt cttgtgattg tttcatatta ggattatttg ttggagcttc
    17581 tactttctct aatcctttcg cataaatcac taaatctgaa tattcacgat tgccatcttg
    17641 tgctggaata tataccacat gatattctgg atcaattaat ttcacaggat cacgtcgttt
    17701 aaaaccagtc aaatctacat ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc
    17761 tggatttcca aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt
    17821 ttcattatca aatacacgat cagactcttc taatgcgcca aagcgtacgg ctcctaaaca
    17881 agtttcatct aatgcttctg taatgtcata attatttgga aattttaatc ccatcttaca
    17941 aaacctcccc aatttttaca ccagttaaaa agacaccaac atcttgatag ccagttgttc
    18001 gacgctctgt ttgtcccata tcatttgttt cacgactatt cactcgttct ttacgaaaaa
    18061 caaaataagc ttcttcaaat gaaatacgca cctctttttc tggtaatcgt aatcttttat
    18121 cctcttcagc tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa cactctgctt
    18181 gtaattgaac atatcgccca gacacaccat taatttcctt tggtggatta ggttcccgtg
    18241 aacccaataa aaatacttga cctaaaaact ctttcttcat acgcaatgct ttttctgcca
    18301 taaagttttt cctcctctta aaaaccaaaa taaattctaa atatttctac attacaatca
    18361 aacaagcggt taaaaatcct aaccgcttgt tcctcatgac taatctttgg ctttacgtac
    18421 tttataaagt gttataccta aagcaatgac taatactact aatccaagaa ccataagtat
    18481 actacttgtt tgttcacctg tttttggcaa cattggtaca acagtagctg gtttaacccg
    18541 ttgttctggt ttcactggtt gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc
    18601 tttgtttgtt agatctacat tgtgtttcgc cacttcttca aacgtttctt tctcatcttc
    18661 cgttttcgct tggagccatt ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc
    18721 cactaagaat tcaaataaga attccatcat ttctttatcg gccgcaaaga ctttttccgc
    18781 atgagtgcct tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata
    18841 gatttgacca atcgcaagac cgtctacttt gacccattca agcacagttt gatttggcaa
    18901 ggccaagatc tcttttccgc catttttatt cgtcgctttc gtcgtaatgg ttggattact
    18961 gattttgatg gtttggtcat tgtcggtaat atctgcgtga gaagccaata atacgccttc
    19021 tctaaacaga tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat
    19081 aaagtctaga ttgactgtac catccgttgt ttctgccaca aacgttaagg tacttgtgac
    19141 ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa caccgttgac
    19201 tgtgtatgtt tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt
    19261 cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg tacgtacact
    19321 tgggttagtt actcgtaccg tttgcccttt gtcgttgatg tctttatggt ttgctagttc
    19381 ttcatcattt ctaaaaagac tttcaaacac gacgatttca cgaccattta tacctgtcaa
    19441 atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa agccaacact
    19501 cgtttctacc gtatttccat gcacaagcaa gggttcaccg gttgctttat ccattaagat
    19561 ccctttgacc aagtatgctt ttcctggaag aatctcggta atttcaacgg tatcgtttaa
    19621 ggtaaacaat cctgttgggt ctgcgactgt caatccgtct tcaaagacag ccgtggtatg
    19681 aacggttgga ttattcactt tcacactttg ggcttcatcg ttgatatccg catgtgctgc
    19741 aacttcaatg ccatcacgga aaagtttttc aaaaacaacg atcgtttgac ctttcagaac
    19801 actagcatta accacaaatt caaggtcttt tgtgccattg actttgtctg caataaaggc
    19861 caaggtactt gtgatttctt tgccatcttg taaaattggc gcattcgttg ccttgttcat
    19921 caatactcct gaaatggtgt attcttttcc gacaaacaag ttggtataag aaacactgtc
    19981 tttaatgacg atcgtttcta atggatcaac cactttttca ccatcggctt tatttgacgc
    20041 ttgtgtttct agttttggtg gaatcaagta attacgtaca gaaaccacta acgtttcccc
    20101 atcatctggc gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg tatttggttg
    20161 gaaactttct aatggggcat tttccaagaa gtaatagtca tcggcaacta gacctttcac
    20221 taaaatttcc ccgttttgat cagtggtata ttcaccaatt gcttgatttt taccgttata
    20281 caaggtaaag ttcacacctg cgagcggcgt tttcttcttg ttccattctt catacttgaa
    20341 taattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga tcggttctgt
    20401 atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta cgggggcttt
    20461 gatttcttcc aaacgatagc gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa
    20521 gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa tctccattgt
    20581 gacataacga ttggctaaag tatcaaaaat cttgaattct gtattagcaa gtggaatcac
    20641 ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta ctttatcttc
    20701 taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg gttcaaatag
    20761 tttataccct tcttttcctt tggtttcaga aagaatgtac gtatcaaaga caacggtatt
    20821 tttaccaaaa gtagcataac catcattgtc tgtcacaacg gttttcacca ctttgccagt
    20881 tgtttttgac gtcaaagtga tttcaatgcc agctaatttt ggtttgttat ctggattcgt
    20941 tgaatcgact aaatctttat tagcaacttt cactaaatca atggttcctt tgatcacttg
    21001 atcttttgcg gtcgcattga cagtttttcc aggttcaaca acaatttcaa ttgcttcatc
    21061 agaaagcaca taaccttttg gcgctttgat ttcttttaaa acatatttgc caggatcaag
    21121 atctttaatt tctgcctgta ctttgccgtt aatttttttc aaggtaattt ctttaatctt
    21181 tttcccatct gcttgataca aaccatagac cgcattttct aaagaagctg caccttgtgc
    21241 tttcacacca gtttccgcat cttctttaaa cacacgaatg tttccttgtt ttagaatttg
    21301 taaattaata acagacgcat taggttctgt tggatgtaac acaccaattg tttgagctcc
    21361 tggacgacga taggctaatg gcgcaccttg aactttaatt ttattcaatt gaatttttgc
    21421 tttatccggt gcctcttttg tgactgtaat ccatacttca ttgccgtgaa tagatacatt
    21481 gacacctttt ggtttttgaa tgtctttcac atatttcaaa aaatcatttt tattctttac
    21541 tttcaacgtt tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc
    21601 aaagttatta atcaaacgtt gaatttctgc ttttcgacga tcataatcaa cacggatatt
    21661 tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg caatgtattg
    21721 ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc ttgttttttt
    21781 ccgtacattt gaatttttca aatagttttt caaagcagta gctgtataaa cttgaccttc
    21841 tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct caaaacgaac
    21901 catataattt aaagtgaagt ttagattgtt cggactagaa gtaacaccaa tattttcgcc
    21961 ccaatcaatt ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga
    22021 acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa tgttctgctc
    22081 ttttttcttt tgattttcaa tactagccgt agattctttg tttggtttcg ttggttgatt
    22141 ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt ttccttcttt
    22201 gtcagcagta gccgctggtt gttccactgt ttcttctatt cgtttctttt cttccgcttg
    22261 tttctcagaa gactctggct gcacagaagg cgttgcttcg gcagcttttt ctccttctag
    22321 agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt
    22381 accaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga
    22441 attgaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac cagctgggac
    22501 cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa tggcttccat
    22561 cgtaggttgt atagccgtcg tttcttctgc atgtgcttga gcagttccca ccaagaaaca
    22621 gaattgagaa aaagccacca atgcgagtgc tttcaaccaa ttcattttcg tttttgcata
    22681 cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacgc cgttaccaaa
    22741 agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc tgaacacatt
    22801 tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc tgcttcgtgg
    22861 aaatcaactg tccatttccc aattggaata ccagcttttt tggcaacttc accatcacca
    22921 aatctcatgc cataccaatt actaccttca tccttaattt ctttccaacc atatgacata
    22981 gcttcatcta atgaatcaaa aatcatgaca ctattgccaa ttgttacagg atctggctta
    23041 agatcttctg gaagagaaat atcaatcata ccataatgat ccgattcctc agtattattg
    23101 tcattactag ctactggttt ggttgttcta ctggcttatt ttgatacaac aagtactctt
    23161 attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca aagtcttgct
    23221 taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc gtaggatcta
    23281 aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg taatctcctt
    23341 caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact tgatttttaa
    23401 ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact ctagattctc
    23461 ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc tacttatgct
    23521 tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac ttcttcatga
    23581 tcgtcccaac caatatcact ctcgaaaaat tccacgttgt tcttatcttt tagctcagta
    23641 aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt
    23701 acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac taaaactttc
    23761 cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac ttattcgctt
    23821 cttctttata aatcgccgat tgtaaaatta agctagacaa ctaaaaaaag tcatttgtgc
    23881 tacactcaaa atagttccga cgaaagaatt ataaggagtg agtacaaatg acctataacc
    23941 atcttacacc aactgaactc gtaatgatag aagcttattt caatcaatca caacctgttt
    24001 caaaagtagc caatctgtta caacgttctc gacaaactat ttataaggtc tatcgttttc
    24061 ttaaatctgg tggaagcgca atgagctact ataaacaata taagaaaaac aaacgtaact
    24121 gcggaagaca tccgattgtt ttacctgatg agcaacagga atacattcaa aaaaaggttg
    24181 ttcaagggtg ggctcctgat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg
    24241 tacgcacact gtatcgaatg tttaaagata aaacctttga cactcacgat ttaccaatga
    24301 agggaaaaag aaaaccaaac ggtcataaag aaaaaagagg aaaacaaggg tttcgccggt
    24361 caatccgaga cagaaagaca gactataccc agtttgatca tgaatttggt cacctggaag
    24421 gtgacactat cgtgggtgga aaacataaaa gcgcggtcat cacattagtt gaacggctat
    24481 ctaaagtcat cattaccttg aaacccgaag gcagacaggc taaagacatt gaaaataggc
    24541 tgaatcaatg gtttcgttgt gtcccaaaaa acttatttaa atccatcaca tttgattgtg
    24601 gaaaagaatt ctcaaattgg aaaaagatta gtaataccaa cgatatttca atttattttg
    24661 cggatccagg aacaccttct caacgaggat tgaatgaaca ttcaaatggc ttattacgta
    24721 aagatggttt gtataaagct atggatttta atggtgtatc tgaaaccttt atccaatcgg
    24781 tggcatcgaa acgaaatcat attcctagaa aatcattaca ctatcggaca ccaatggaag
    24841 tatttttgag ttacgtcaca aaagatgatt tgtctaactt aatttgacaa acgaaaaata
    24901 aaaaaactcc aataatcgga aattactatc ttttcctcat tcctttttta aatcttcata
    24961 cttcttctta tctttgtcct aacctaataa cacaaatttt gaagaaaaac tcagtaaaac
    25021 ctgattcttt ttttatcttc acaacaacca actttttctt cagatccttt tactagaact
    25081 tgtatacaaa aattgttgaa tgctgatata atttgcggct acttgaaaaa acgatagatg
    25141 taaaaagata aatagaatca ttttttattc tatatcagtc tatgattcat tgcttatcaa
    25201 tcacccaacc cattcgatgc cgtttttttt agagaaattt aaaaaagacc gtacgaatct
    25261 cgcattatcc gattactttt ccagaaaaga tccccaattc tgtataaata accctatctt
    25321 cttgttggtt tcattttctc atataagaat tcagtcaatc tacccactat gaccattact
    25381 acgtccaaca caactgacat attaaccacg taagtattcc ctcacagatc tccgaatctc
    25441 catggtatgg attcccatcc tgacgctgca gaggacaaca tatttcatgt ggctctgtac
    25501 ccttttgatt tggaaacact acaaatcaat actcatctat aacttatcaa gcgaactttc
    25561 gctaggcttc tagtgattac taaattgttg gataacatac atagcccatt ttatctagct
    25621 gttattcata acagttagtt cttgtattac ttcttccgct tgtgttgcca ttgagcttgt
    25681 caaaaagtta cttagaaaaa agctgccagt aaagcaactt tcaacaaacc ctttttgttt
    25741 cttatcatat caagaaacct cct
//
LOCUS       DAZKCE010000031        25727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000031 DAZKCE010000000
VERSION     DAZKCE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.031"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..2230
                     /locus_tag="ACG8KQ_001135"
     CDS             170..2230
                     /locus_tag="ACG8KQ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9104079.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2313..2882
                     /locus_tag="ACG8KQ_001136"
     CDS             2313..2882
                     /locus_tag="ACG8KQ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104080.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3057..3863)
                     /locus_tag="ACG8KQ_001137"
     CDS             complement(3057..3863)
                     /locus_tag="ACG8KQ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104081.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4001..5515
                     /locus_tag="ACG8KQ_001138"
     CDS             4001..5515
                     /locus_tag="ACG8KQ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9104082.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5918..6901)
                     /locus_tag="ACG8KQ_001139"
     CDS             complement(5918..6901)
                     /locus_tag="ACG8KQ_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9104083.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6906..7553)
                     /locus_tag="ACG8KQ_001140"
     CDS             complement(6906..7553)
                     /locus_tag="ACG8KQ_001140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9104084.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7638..8270)
                     /locus_tag="ACG8KQ_001141"
     CDS             complement(7638..8270)
                     /locus_tag="ACG8KQ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9104085.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8286..8837)
                     /gene="coaC"
                     /locus_tag="ACG8KQ_001142"
     CDS             complement(8286..8837)
                     /gene="coaC"
                     /locus_tag="ACG8KQ_001142"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9104086.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8834..9571)
                     /locus_tag="ACG8KQ_001143"
     CDS             complement(8834..9571)
                     /locus_tag="ACG8KQ_001143"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9104087.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9568..10557)
                     /locus_tag="ACG8KQ_001144"
     CDS             complement(9568..10557)
                     /locus_tag="ACG8KQ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9104088.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10815..11357
                     /locus_tag="ACG8KQ_001145"
     CDS             10815..11357
                     /locus_tag="ACG8KQ_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9104089.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11374..12459
                     /locus_tag="ACG8KQ_001146"
     CDS             11374..12459
                     /locus_tag="ACG8KQ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104090.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12471..13277
                     /locus_tag="ACG8KQ_001147"
     CDS             12471..13277
                     /locus_tag="ACG8KQ_001147"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104091.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13278..14105
                     /locus_tag="ACG8KQ_001148"
     CDS             13278..14105
                     /locus_tag="ACG8KQ_001148"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104092.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14102..15175
                     /locus_tag="ACG8KQ_001149"
     CDS             14102..15175
                     /locus_tag="ACG8KQ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9104093.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15603..18299
                     /locus_tag="ACG8KQ_001150"
     CDS             15603..18299
                     /locus_tag="ACG8KQ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9104094.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18364..18864
                     /locus_tag="ACG8KQ_001151"
     CDS             18364..18864
                     /locus_tag="ACG8KQ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9104095.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18876..19370
                     /locus_tag="ACG8KQ_001152"
     CDS             18876..19370
                     /locus_tag="ACG8KQ_001152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9104096.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19391..20200
                     /locus_tag="ACG8KQ_001153"
     CDS             19391..20200
                     /locus_tag="ACG8KQ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9104097.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20197..21018
                     /locus_tag="ACG8KQ_001154"
     CDS             20197..21018
                     /locus_tag="ACG8KQ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9104098.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21069..23168
                     /locus_tag="ACG8KQ_001155"
     CDS             21069..23168
                     /locus_tag="ACG8KQ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9104099.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23165..24283
                     /locus_tag="ACG8KQ_001156"
     CDS             23165..24283
                     /locus_tag="ACG8KQ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9104100.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24322..25200
                     /locus_tag="ACG8KQ_001157"
     CDS             24322..25200
                     /locus_tag="ACG8KQ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9104101.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25346..25543
                     /locus_tag="ACG8KQ_001158"
     CDS             25346..25543
                     /locus_tag="ACG8KQ_001158"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9104102.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25527..>25727
                     /locus_tag="ACG8KQ_001159"
     CDS             25527..>25727
                     /locus_tag="ACG8KQ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104103.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVV"
BASE COUNT         8142 a         4470 c         5429 g         7686 t
ORIGIN      
        1 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
       61 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
      121 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
      181 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
      241 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
      301 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
      361 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
      421 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
      481 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
      541 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
      601 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
      661 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
      721 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
      781 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
      841 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
      901 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
      961 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     1021 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     1081 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     1141 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     1201 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     1261 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     1321 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     1381 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     1441 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     1501 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     1561 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     1621 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     1681 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     1741 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     1801 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     1861 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     1921 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     1981 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     2041 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     2101 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     2161 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     2221 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     2281 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     2341 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     2401 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     2461 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     2521 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     2581 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     2641 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     2701 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     2761 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     2821 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     2881 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     2941 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     3001 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     3061 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     3121 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     3181 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     3241 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     3301 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     3361 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     3421 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     3481 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     3541 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     3601 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     3661 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     3721 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     3781 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     3841 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     3901 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     3961 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     4021 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     4081 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     4141 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     4201 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     4261 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     4321 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     4381 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     4441 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     4501 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     4561 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     4621 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     4681 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     4741 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     4801 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     4861 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     4921 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     4981 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     5041 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     5101 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     5161 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     5221 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     5281 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     5341 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     5401 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     5461 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     5521 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     5581 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     5641 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     5701 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     5761 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     5821 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     5881 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     5941 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     6001 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     6061 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     6121 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     6181 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     6241 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     6301 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     6361 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     6421 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     6481 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     6541 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     6601 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     6661 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     6721 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     6781 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     6841 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     6901 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     6961 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     7021 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     7081 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     7141 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     7201 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     7261 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     7321 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     7381 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     7441 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     7501 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     7561 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     7621 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     7681 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     7741 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     7801 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     7861 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     7921 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     7981 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     8041 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     8101 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
     8161 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
     8221 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
     8281 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
     8341 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
     8401 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
     8461 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
     8521 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
     8581 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
     8641 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
     8701 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
     8761 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
     8821 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
     8881 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
     8941 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
     9001 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
     9061 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
     9121 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
     9181 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
     9241 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
     9301 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
     9361 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
     9421 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
     9481 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
     9541 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
     9601 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
     9661 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
     9721 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
     9781 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
     9841 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
     9901 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
     9961 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    10021 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    10081 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    10141 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    10201 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    10261 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    10321 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    10381 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    10441 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    10501 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    10561 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    10621 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    10681 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    10741 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    10801 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    10861 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    10921 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    10981 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    11041 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    11101 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    11161 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    11221 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    11281 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    11341 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    11401 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    11461 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    11521 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    11581 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    11641 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    11701 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    11761 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    11821 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    11881 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    11941 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    12001 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    12061 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    12121 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    12181 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    12241 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    12301 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    12361 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    12421 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    12481 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    12541 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    12601 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    12661 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    12721 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    12781 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    12841 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    12901 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    12961 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    13021 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    13081 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    13141 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    13201 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    13261 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    13321 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    13381 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    13441 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    13501 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    13561 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    13621 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    13681 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    13741 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    13801 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    13861 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    13921 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    13981 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    14041 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    14101 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    14161 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    14221 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    14281 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    14341 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    14401 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    14461 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    14521 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    14581 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    14641 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    14701 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    14761 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    14821 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    14881 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    14941 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    15001 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    15061 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    15121 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    15181 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    15241 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    15301 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    15361 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    15421 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    15481 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    15541 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    15601 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    15661 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    15721 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    15781 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    15841 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    15901 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    15961 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    16021 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    16081 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    16141 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    16201 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    16261 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    16321 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    16381 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    16441 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    16501 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    16561 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    16621 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    16681 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    16741 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    16801 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    16861 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    16921 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    16981 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    17041 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    17101 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    17161 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    17221 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    17281 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    17341 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    17401 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    17461 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    17521 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    17581 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    17641 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    17701 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    17761 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    17821 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    17881 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    17941 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    18001 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    18061 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    18121 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    18181 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    18241 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    18301 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    18361 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    18421 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    18481 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    18541 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    18601 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    18661 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    18721 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    18781 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    18841 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    18901 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    18961 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    19021 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    19081 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    19141 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    19201 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    19261 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    19321 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    19381 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg
    19441 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    19501 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    19561 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    19621 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    19681 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    19741 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    19801 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    19861 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    19921 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    19981 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    20041 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    20101 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    20161 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    20221 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    20281 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    20341 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    20401 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    20461 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    20521 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    20581 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    20641 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    20701 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    20761 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    20821 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    20881 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    20941 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    21001 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    21061 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    21121 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    21181 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    21241 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    21301 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    21361 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    21421 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    21481 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    21541 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    21601 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    21661 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    21721 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    21781 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    21841 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    21901 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    21961 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    22021 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    22081 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    22141 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    22201 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    22261 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    22321 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    22381 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    22441 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    22501 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    22561 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    22621 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    22681 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    22741 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    22801 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    22861 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    22921 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    22981 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    23041 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    23101 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    23161 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    23221 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    23281 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    23341 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    23401 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    23461 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    23521 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    23581 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    23641 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    23701 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    23761 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    23821 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    23881 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    23941 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    24001 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    24061 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    24121 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    24181 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    24241 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    24301 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    24361 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    24421 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    24481 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    24541 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    24601 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    24661 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    24721 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    24781 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    24841 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    24901 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    24961 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    25021 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    25081 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    25141 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    25201 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    25261 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    25321 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    25381 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    25441 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    25501 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    25561 ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa
    25621 aaaagaaagt ttctatacat tttaaatatt ttattcctat ttctattgct aaaaaagtag
    25681 aaattctata tttaccttta actctcgctt ctcttgcaaa tgtagtt
//
LOCUS       DAZKCE010000032        25243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000032 DAZKCE010000000
VERSION     DAZKCE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.032"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..3427
                     /locus_tag="ACG8KQ_001160"
     CDS             149..3427
                     /locus_tag="ACG8KQ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9104104.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3968..5875)
                     /locus_tag="ACG8KQ_001161"
     CDS             complement(3968..5875)
                     /locus_tag="ACG8KQ_001161"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9104105.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6005..6880
                     /locus_tag="ACG8KQ_001162"
     CDS             6005..6880
                     /locus_tag="ACG8KQ_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9104106.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6960..7934
                     /locus_tag="ACG8KQ_001163"
     CDS             6960..7934
                     /locus_tag="ACG8KQ_001163"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9104107.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8114..9259
                     /locus_tag="ACG8KQ_001164"
     CDS             8114..9259
                     /locus_tag="ACG8KQ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9104108.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9396..9740
                     /locus_tag="ACG8KQ_001165"
     CDS             9396..9740
                     /locus_tag="ACG8KQ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9104109.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10011..11297
                     /gene="efmA"
                     /locus_tag="ACG8KQ_001166"
     CDS             10011..11297
                     /gene="efmA"
                     /locus_tag="ACG8KQ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP9104110.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11435..12034)
                     /locus_tag="ACG8KQ_001167"
     CDS             complement(11435..12034)
                     /locus_tag="ACG8KQ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9104111.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12438..13559
                     /gene="mnmA"
                     /locus_tag="ACG8KQ_001168"
     CDS             12438..13559
                     /gene="mnmA"
                     /locus_tag="ACG8KQ_001168"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9104112.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13805..15226
                     /gene="arcD"
                     /locus_tag="ACG8KQ_001169"
     CDS             13805..15226
                     /gene="arcD"
                     /locus_tag="ACG8KQ_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9104113.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15240..16358
                     /locus_tag="ACG8KQ_001170"
     CDS             15240..16358
                     /locus_tag="ACG8KQ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9104114.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16418..17710)
                     /locus_tag="ACG8KQ_001171"
     CDS             complement(16418..17710)
                     /locus_tag="ACG8KQ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9104115.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17860..18420
                     /locus_tag="ACG8KQ_001172"
     CDS             17860..18420
                     /locus_tag="ACG8KQ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9104116.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18549..18926
                     /locus_tag="ACG8KQ_001173"
     CDS             18549..18926
                     /locus_tag="ACG8KQ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9104117.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18859..20931
                     /locus_tag="ACG8KQ_001174"
     CDS             18859..20931
                     /locus_tag="ACG8KQ_001174"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9104118.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21142..22089)
                     /locus_tag="ACG8KQ_001175"
     CDS             complement(21142..22089)
                     /locus_tag="ACG8KQ_001175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9104119.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22335..22799
                     /locus_tag="ACG8KQ_001176"
     CDS             22335..22799
                     /locus_tag="ACG8KQ_001176"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9104120.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22861..23904)
                     /locus_tag="ACG8KQ_001177"
     CDS             complement(22861..23904)
                     /locus_tag="ACG8KQ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9104121.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24281..>25243)
                     /locus_tag="ACG8KQ_001178"
     CDS             complement(24281..>25243)
                     /locus_tag="ACG8KQ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9104122.1"
                     /translation="KNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMIL
                     MIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLC
                     AVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFW
                     ISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVV
                     ALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSS
                     VLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         8260 a         4613 c         5290 g         7080 t
ORIGIN      
        1 aataaatctc tttattatca acaacttaag ttatattttt tctttttata aaatacactt
       61 attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt aagtaaacgc
      121 tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat acagtgcgct
      181 aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg aagttgctgc
      241 taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac
      301 aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa atgatcctat
      361 gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg
      421 gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg aaccattaga
      481 agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga
      541 tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga tgcgggcgac
      601 gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt taatggtaag
      661 agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt catttgccat
      721 actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg tcaacacgag
      781 cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag gaatatcatc
      841 tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa cacctttaga
      901 gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg gaaacggtca
      961 tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga tgaaaaaaac
     1021 gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa tggcttctga
     1081 catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt atgcaggatt
     1141 tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt actcagatcc
     1201 tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga tttacactgt
     1261 gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag caaatgctga
     1321 cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg caaaaaactt
     1381 ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag gaacgaacga
     1441 atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg tagagatgac
     1501 tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta aatattcgac
     1561 agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa atccattatc
     1621 tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg cgcctggaac
     1681 ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc
     1741 gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg attttaaagg
     1801 aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt ttgacattac
     1861 aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt tgatcgaaga
     1921 gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct caaatgatcc
     1981 tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg aaaatgccga
     2041 tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag aaggaaatgt
     2101 atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt ttgctaaaaa
     2161 tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc gaaacggctg
     2221 ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg gttcttcttt
     2281 aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga aaggaatcga
     2341 ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata acggttcata
     2401 taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt ctggtatctt
     2461 atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga atcaaaacga
     2521 agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac aatcagaaaa
     2581 tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat gttcgaaaga
     2641 aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg gactgatgca
     2701 gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc cattatctgt
     2761 aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt atgcaatcgt
     2821 acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta tacaagaagc
     2881 aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt
     2941 tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc gttatttccc
     3001 agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc ataccgctat
     3061 tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt cggaaatatt
     3121 tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag cgatcactgt
     3181 gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa
     3241 tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga cagcaaaaga
     3301 aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata acccgttgct
     3361 agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa
     3421 aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa agtaaaaata
     3481 gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga tgaggttctc
     3541 cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt tcacaatctc
     3601 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac ggcaaaatat
     3661 gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga tttcacaacc
     3721 tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt atgcaaaaat
     3781 gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc tttttcacaa
     3841 cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa
     3901 aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc acttctggca
     3961 caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg tcttgttccg
     4021 tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt
     4081 tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt tttgcttttg
     4141 tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct gattccgttt
     4201 ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc gaaacggtta
     4261 attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg atcattgctt
     4321 gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc aagttgccat
     4381 attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga gcgataacag
     4441 ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat ggtgcttgaa
     4501 ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca ttgaccgtgt
     4561 cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg aaacgatctt
     4621 ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc tgatctgcag
     4681 gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct acttggatac
     4741 ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc cagtcatttt
     4801 cttctagacg ttttttataa acggcgatca tttttttcac catttgttct acgtcttttt
     4861 tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga tcgaattgtc
     4921 cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt ccagacaata
     4981 cgcgagaata gattccgctg acttggcgga attcttctga tagataacca ctaagagaac
     5041 gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct gtcaggatgt
     5101 cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga gttgtcccaa
     5161 tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata aatgtatcaa
     5221 aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt tttacatgag
     5281 gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc tgactttttt
     5341 ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct attagttgtg
     5401 caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa gaaggcggat
     5461 aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc agattaaatg
     5521 gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg tcgaaagact
     5581 gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac tcatttcttc
     5641 ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt tctgaagaca
     5701 ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg tcgatcccgt
     5761 caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt gctgtttttt
     5821 gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg ttcattcatc
     5881 gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata
     5941 gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca atccttcact
     6001 tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac ctttatattt
     6061 caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt caagaaaaag
     6121 taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta tcgatcaatc
     6181 aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc tttcttgtaa
     6241 aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca acagaaaaga
     6301 agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt
     6361 gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct caggctatct
     6421 ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc gtaaggggtg
     6481 gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc
     6541 attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg ttagtacagg
     6601 gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg
     6661 ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt
     6721 gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt attttgaaac
     6781 tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc ataaagtcat
     6841 tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg aaattaacag
     6901 aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg gaggactaca
     6961 tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc aaccgtccat
     7021 tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat
     7081 ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat catgtgtata
     7141 tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg atcatgatcg
     7201 atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac tatgggtatg
     7261 ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg atcgctaact
     7321 tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg gttcaagtac
     7381 aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt gctcattatt
     7441 accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg
     7501 ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc gtagatcatg
     7561 cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga aagacatgta
     7621 tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca aatgtattaa
     7681 aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg tccgatcatg
     7741 cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat tctgttttca
     7801 cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa ttaatgggtg
     7861 aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt ctggaagaaa
     7921 aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg agcagcttca
     7981 aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga aacgtcctct
     8041 agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg tgcctagcaa
     8101 taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg caactacccc
     8161 catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt ttggaaatcc
     8221 ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat
     8281 cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg ggggaacaga
     8341 aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag gcagacatct
     8401 aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt tagagacaaa
     8461 ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt
     8521 caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga acaatgaaat
     8581 cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc cagcttattt
     8641 ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag aattaggaat
     8701 cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg gtttcttata
     8761 taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg aagaaaagcg
     8821 gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag ttgaactgct
     8881 gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc aattgattct
     8941 tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa cgaataaact
     9001 ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac tgatgaagct
     9061 tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa atatcgagcc
     9121 ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag aatccatccg
     9181 tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg atcaattagt
     9241 tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt ctgagagaac
     9301 gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat aaacttttat
     9361 aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca acagcaacgg
     9421 tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat acattgcgcg
     9481 ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca ttagatgcca
     9541 cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat attcaaacat
     9601 tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc aaagatccaa
     9661 aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt agccgtgggt
     9721 tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg aaacggccta
     9781 ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt atttttaaat
     9841 ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt aaagaaattt
     9901 aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa
     9961 tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt atggaaaatg
    10021 aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta tcgggacagt
    10081 ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat ttgacaagag
    10141 aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt cccatggttt
    10201 tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct cttttgattg
    10261 tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga acgatttcag
    10321 aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat
    10381 tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct cacataacaa
    10441 ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg ccagctctag
    10501 gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat gtattggggg
    10561 cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct cctgaaatat
    10621 tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa ttgatggata
    10681 ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg ttcatgccag
    10741 caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt ggacaggcgg
    10801 gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg atcagtttta
    10861 taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt atgggcctga
    10921 cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac tttcttatct
    10981 tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg gcaatgatcc
    11041 agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac tctttgatga
    11101 acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt ttaggggtag
    11161 aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt ttatttttaa
    11221 cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa gaacaccatg
    11281 agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac aactccattt
    11341 ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga gtaattatct
    11401 ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac ttcctctttg
    11461 cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt agtttttcct
    11521 ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg ataggtacgc
    11581 tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg agcaacatcg
    11641 gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct acatcataaa
    11701 aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa taacggaaaa
    11761 aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt tgagtgacca
    11821 gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg tcgcctagat
    11881 gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac tgttggttca
    11941 tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat aagcgttcat
    12001 ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc ttttttctta
    12061 gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa ggttgtaaat
    12121 gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc acaatttata
    12181 aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat tttttatatc
    12241 gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac tgaataggtc
    12301 cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag aaatatgacg
    12361 tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca gaatctaatt
    12421 gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta gggatgagcg
    12481 gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac gacgtgatcg
    12541 gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg gccacggaag
    12601 attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac tctgtgaatt
    12661 ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac cgcgcaggac
    12721 gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca ttcttggatt
    12781 atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa gtcactcgcg
    12841 atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat caaacttact
    12901 tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta ggcggcatgg
    12961 aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct aagaaaaaag
    13021 attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg agcaactatc
    13081 tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga caacatgctg
    13141 gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc ggaggagatt
    13201 cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta tatgtggggc
    13261 aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa atccacttta
    13321 cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt tatcgtcagc
    13381 aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc gtattcgacg
    13441 aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga atggaatgtc
    13501 tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa tacgtttaag
    13561 cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt cttttggata
    13621 gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg aaatacttgt
    13681 gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa gcaagagcag
    13741 ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag tgagggtgat
    13801 ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc tggttgtcag
    13861 ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt ctgcagctcc
    13921 agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt tggtattatc
    13981 gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg gttacgcgga
    14041 agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt tgtctgcgtg
    14101 gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat ttttcccgat
    14161 attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta tttggggtct
    14221 gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat
    14281 ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat ttaaagtgaa
    14341 tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg gaacagggca
    14401 gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt gggtctttgt
    14461 agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag aagcccagca
    14521 agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt cgatcttgcc
    14581 atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta tggcaaatat
    14641 tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct tgattatttc
    14701 gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga tgttgatggc
    14761 tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta
    14821 ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct tagttactga
    14881 ttatgcctat caatttgcct attctttatg tacagcggca attttgattt gttatctact
    14941 tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac aagtagtgat
    15001 cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat tttcttatgt
    15061 ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg cttgtcgtga
    15121 aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc
    15181 actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg gaaagaaaca
    15241 tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag aaagcggtat
    15301 atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt ggcttagatg
    15361 ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac tatggctgga
    15421 tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg
    15481 aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta tacgcgttag
    15541 tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta tatgacatcc
    15601 ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa gattggcagt
    15661 ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc tgtttgaata
    15721 gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa atcgcagaaa
    15781 ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct ttaacggata
    15841 aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc aattccctat
    15901 ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga cctgagttgg
    15961 cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc ctgtcagatg
    16021 atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca
    16081 ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg
    16141 tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa gatgaccaga
    16201 cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga agtgcctttg
    16261 atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg gaaaagggat
    16321 tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg caaggacagt
    16381 ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga gaaggagagt
    16441 gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc tgatggatct
    16501 cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa
    16561 agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc cctactacgt
    16621 taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct tgatgaggcg
    16681 aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag ataccaatca
    16741 aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg atataaacca
    16801 gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg tcacctaagc
    16861 gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca atgattccgc
    16921 tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga ctgatgattg
    16981 gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata ccgatgacga
    17041 cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt tcttttttca
    17101 ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa aataagatga
    17161 tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc ccgccaagaa
    17221 gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga acttgaccac
    17281 ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata taaccagaaa
    17341 aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg caacccatca
    17401 aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt cttccttttt
    17461 ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa gtagatgaaa
    17521 aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac gtatctatgt
    17581 aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat gttccaaacc
    17641 ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt tcctttattc
    17701 ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt tattataaac
    17761 tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg tggtaaactg
    17821 aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag aaatctgtct
    17881 caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt tcagcaaata
    17941 acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta taaccatttc
    18001 aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga acttaatgac
    18061 cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa
    18121 acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg
    18181 cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat cattttgcag
    18241 atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt
    18301 agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa agaaccaaca
    18361 atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac tttgccttaa
    18421 acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt ttataagaga
    18481 aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct gaaagaagga
    18541 gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg tggttttttg
    18601 aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa gaagcacgac
    18661 aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa
    18721 gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg
    18781 atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag
    18841 aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa
    18901 gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga aggacccaag
    18961 gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga agatctgaat
    19021 aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa actaggaatc
    19081 aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc tcagcgaaaa
    19141 gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact tgttgctcgc
    19201 tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat ctcttctcaa
    19261 acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga atatgatgca
    19321 ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc
    19381 agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca gacaccgaca
    19441 ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca aacgtatttc
    19501 acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct
    19561 ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag caaaggaact
    19621 gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta tgatttaaca
    19681 gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac attaagtctt
    19741 gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga cagcaaatat
    19801 ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag cgactttgct
    19861 cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa agtattccaa
    19921 gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc acgttatgaa
    19981 aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt tttaggactg
    20041 tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg taaagaacga
    20101 ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag
    20161 acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa
    20221 gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca aatggaaaaa
    20281 tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat caaatctgaa
    20341 ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca attattagta
    20401 ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa agaccttgaa
    20461 gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga aaaagaaaca
    20521 aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt ctcgattacc
    20581 caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa
    20641 atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc aaaagtctct
    20701 aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct
    20761 tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa agagcagaag
    20821 aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa
    20881 ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta ataagatatg
    20941 aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc
    21001 ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg gaacaccatt
    21061 tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat ttaaaaaaat
    21121 gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct tcactttttc
    21181 tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt tcccaatata
    21241 tttcccgatg attccaagac ataacaattg gattccacca atgaacagga agatcgagac
    21301 catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa taatgaacaa
    21361 aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa tcgttaacgg
    21421 tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc agaaggacca
    21481 agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg ttttaaatcc
    21541 gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta ggacggcatc
    21601 caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt ctgtatcgct
    21661 gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc
    21721 ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga tccggttgat
    21781 catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg tcactaaatc
    21841 tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat ttctggaaaa
    21901 tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg ctaacgtacc
    21961 gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt tgaatttttc
    22021 aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga caacaattga
    22081 tactttcatc gtttctatcc tttcaatcgt atgtttatat atggtttaca ttcaaaattt
    22141 aataaacctt aaataacacc tttaaaattt gattttacgt aaaagaacag gtattctttt
    22201 tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt atcaaacttt taagaacaat
    22261 ttttattagt tggcaagtag ttttggatag agaaaaagga ggaagttcca gtctattgac
    22321 taggagcaaa caaaatgaaa atacacacac ctatcggctt tttatggtta gatgcagatg
    22381 aatccggatt gactgctgta tcttttcagc caatttcaga aagcgaatca gcgaatcagc
    22441 gaattttaac gcgcacacaa gaacagtttg aagaatattt tgcgggaaaa agaaaagaat
    22501 ttgatgttcc tttatctata aagagaggta cgccttttca gcaaaatgtc tggaaagctt
    22561 tgtcgaccat tccttatgga gaaatccgaa cgtataaaga agtagccgtt gctgttgata
    22621 gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac caatccattg ccaatcgtta
    22681 ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa
    22741 tagatggggt tgaaatcaaa cgccaattgt tgttgttaga aggatatctg caaaattaaa
    22801 aacagttgtg attcggacat tgttccatgc gagaatcaca actgtttttt catgttcatt
    22861 ctaaactttg atcagccaaa taaatagtaa agccatcact gtttggagaa gcccgacagc
    22921 taatccataa gataagaagc gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag
    22981 accaattgcc gctaaagcag tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc
    23041 aataactaca ggtaaagaaa acaagctgtt gaccaaacaa aagatcacaa accctaatac
    23101 ataccaagga agcggtaatt ttcgtttttt ctgcgtatca gttggcgttt caaacagagc
    23161 tggttgtttc ttgtgcatga atttttcaaa cagatagaca acaacaacga gcatcataat
    23221 acgtagaatc ttaaatagca tggcgaattc gacaacagaa ttgtcaagta agcttgcccc
    23281 agcgactact tgaccaacag attggagtgt accacccaaa agtgcacttt tttcaatgag
    23341 atttccacca aacaactgta atccaagaaa agggagagtc aacatcatca cagtacccag
    23401 tagattgact agtgtgatga tctgtccttt ttcttcctct ttcgcatgga tggaaggggc
    23461 gattgaagca atagcagaag aaccacaaac agcgtttcct ccagccatca tcaaagaaga
    23521 agtatcgctg aattgcagtt ttttaccaat caagtaagca ccgatgatag tgactgacat
    23581 ttgcagtaac acaaaaacaa ttccttttac accaatttga gcaatcgttt gagctgtgac
    23641 agttgcacct agtaaaaaaa cagagaattc taatagtttg ctttcagcaa attttgtgcc
    23701 gattgctaga acaggctgtt taaaaaaagt atttcctaat aaaataccca ataaaatagc
    23761 aatagtcgct ccacctaact gtggcagata aatagcaaga agcctgctga aggcagcgat
    23821 ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc gctgaatcct ttacagattt
    23881 tattggatgt gacaatgatt tcatttgatc ggttcctttc gtcaataaat actaaaagca
    23941 tactcgaaca tagtagcact aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa
    24001 agtcttgtac gacctcttgt ttcgaataaa cggtgttcca gaagtagctt ctacggaaat
    24061 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    24121 agctgaccac ttcttttacg acctcttggt aaacggtgtt caaaagcttg catctgcggt
    24181 aataagccca accagtacaa aaacatgaac agctgttttt gtttggccgt tcttattact
    24241 tgatgcagaa cagctttttc acaacctatt gaatcgatta ttaagcgatt tttttagaaa
    24301 caaattttcc atgtttgcta tgttctttaa ttaagaaacg tacgtaatga gcaagaacgt
    24361 agaagacaac tacacttgca aacagacatt ccgcaacttt taagaaatcc aatacgctac
    24421 tatagatacc taaactgttg aagtaatgaa cagtagtaaa tatagctgtt gcagagataa
    24481 caagcgggaa tgtaaaggct gcatagcttg gataaaaacg aagcttgaac aaacgtggaa
    24541 gcatagacag cacaacgaaa tacaatactt gagataaagc gaacaatgct acgacaagat
    24601 aaaaattagg ttcagcaaag tttttaagat aacctgttag gcaaagagaa ccaggtgctg
    24661 caacgatcgt aatcagcgga cgtgtcggat gagccatatt tcttacgaaa aagacacgtt
    24721 tgatgatgat cggcaataag aacgcatagc agatcaatga aatccaaaag aaaatttttc
    24781 caataagtgg gaagaagttc ccggcagtta cagtgatgac cccaaatcca acgtaaatga
    24841 taaaccagct tggataaatt gcttcaattg aaagatcagc tttgataaca tggtgataat
    24901 tgaaataaaa gacaaggatc acttggaaga taacagcgca cagccaaatg aatttgacaa
    24961 ttggtgcaat actttcatat gaaacaaaat aagtacaaat aaccatgagt gacatcgtaa
    25021 aagttggtga aacggaggcg atgattggat catgcaacgt ttgtttcgtg tgttcgaata
    25081 agataaacaa tttaacaaaa atgaatatca taagaatcat agagataccg ccagttagat
    25141 tgcctaaagc aacatggtga tagtctttaa acagatttcc aagggaagct aacccaagga
    25201 tcaacccgca gatcgggatc ggaataattt tcaaaaagtt ctt
//
LOCUS       DAZKCE010000033        25214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000033 DAZKCE010000000
VERSION     DAZKCE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.033"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8KQ_001179"
     CDS             complement(164..388)
                     /locus_tag="ACG8KQ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP9104123.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8KQ_001180"
     CDS             complement(586..2166)
                     /locus_tag="ACG8KQ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9104124.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8KQ_001181"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8KQ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9104125.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8KQ_001182"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8KQ_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9104126.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8KQ_001183"
     CDS             5331..5765
                     /locus_tag="ACG8KQ_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104127.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8KQ_001184"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8KQ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9104128.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8KQ_001185"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8KQ_001185"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9104129.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8KQ_001186"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8KQ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9104130.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8KQ_001187"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8KQ_001187"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9104131.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8KQ_001188"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8KQ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9104132.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8KQ_001189"
     CDS             11001..11471
                     /locus_tag="ACG8KQ_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP9104133.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8KQ_001190"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8KQ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9104134.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8KQ_001191"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8KQ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9104135.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8KQ_001192"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8KQ_001192"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9104136.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8KQ_001193"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8KQ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9104137.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8KQ_001194"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8KQ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9104138.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8KQ_001195"
     CDS             16164..16967
                     /locus_tag="ACG8KQ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9104139.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8KQ_001196"
     CDS             17145..18092
                     /locus_tag="ACG8KQ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9104140.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8KQ_001197"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8KQ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9104141.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8KQ_001198"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8KQ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9104142.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8KQ_001199"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8KQ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9104143.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8KQ_001200"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8KQ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9104144.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8KQ_001201"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8KQ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9104145.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8KQ_001202"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8KQ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9104146.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8KQ_001203"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8KQ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9104147.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACG8KQ_001204"
     CDS             23883..25052
                     /locus_tag="ACG8KQ_001204"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9104148.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7575 a         5086 c         4060 g         8493 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgt
//
LOCUS       DAZKCE010000034        25109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000034 DAZKCE010000000
VERSION     DAZKCE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.034"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1745
                     /locus_tag="ACG8KQ_001205"
     CDS             105..1745
                     /locus_tag="ACG8KQ_001205"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9104149.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1899..3221
                     /locus_tag="ACG8KQ_001206"
     CDS             1899..3221
                     /locus_tag="ACG8KQ_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9104150.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3283..3714)
                     /locus_tag="ACG8KQ_001207"
     CDS             complement(3283..3714)
                     /locus_tag="ACG8KQ_001207"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104151.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3778..4614)
                     /locus_tag="ACG8KQ_001208"
     CDS             complement(3778..4614)
                     /locus_tag="ACG8KQ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9104152.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4804..5106)
                     /locus_tag="ACG8KQ_001209"
     CDS             complement(4804..5106)
                     /locus_tag="ACG8KQ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9104153.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5132..5563)
                     /gene="ruvX"
                     /locus_tag="ACG8KQ_001210"
     CDS             complement(5132..5563)
                     /gene="ruvX"
                     /locus_tag="ACG8KQ_001210"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9104154.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5560..5826)
                     /locus_tag="ACG8KQ_001211"
     CDS             complement(5560..5826)
                     /locus_tag="ACG8KQ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9104155.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6080..6805)
                     /locus_tag="ACG8KQ_001212"
     CDS             complement(6080..6805)
                     /locus_tag="ACG8KQ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9104156.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7171..7241)
                     /locus_tag="ACG8KQ_001213"
     tRNA            complement(7171..7241)
                     /locus_tag="ACG8KQ_001213"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7207..7209),aa:Gly,seq:ccc)
     gene            7358..8110
                     /locus_tag="ACG8KQ_001214"
     CDS             7358..8110
                     /locus_tag="ACG8KQ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9104157.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8126..8605
                     /locus_tag="ACG8KQ_001215"
     CDS             8126..8605
                     /locus_tag="ACG8KQ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9104158.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8856..10436)
                     /locus_tag="ACG8KQ_001216"
     CDS             complement(8856..10436)
                     /locus_tag="ACG8KQ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9104159.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10429..11172)
                     /locus_tag="ACG8KQ_001217"
     CDS             complement(10429..11172)
                     /locus_tag="ACG8KQ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104160.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11348..12124)
                     /locus_tag="ACG8KQ_001218"
     CDS             complement(11348..12124)
                     /locus_tag="ACG8KQ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9104161.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12372..13208)
                     /locus_tag="ACG8KQ_001219"
     CDS             complement(12372..13208)
                     /locus_tag="ACG8KQ_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9104162.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13219..14148)
                     /locus_tag="ACG8KQ_001220"
     CDS             complement(13219..14148)
                     /locus_tag="ACG8KQ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9104163.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14340..14693
                     /locus_tag="ACG8KQ_001221"
     CDS             14340..14693
                     /locus_tag="ACG8KQ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9104164.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14767..15423)
                     /locus_tag="ACG8KQ_001222"
     CDS             complement(14767..15423)
                     /locus_tag="ACG8KQ_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9104165.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15649..17583
                     /locus_tag="ACG8KQ_001223"
     CDS             15649..17583
                     /locus_tag="ACG8KQ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9104166.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17669..18892)
                     /locus_tag="ACG8KQ_001224"
     CDS             complement(17669..18892)
                     /locus_tag="ACG8KQ_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9104167.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(18886..20757)
                     /locus_tag="ACG8KQ_001225"
     CDS             complement(18886..20757)
                     /locus_tag="ACG8KQ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9104168.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20778..21896)
                     /locus_tag="ACG8KQ_001226"
     CDS             complement(20778..21896)
                     /locus_tag="ACG8KQ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9104169.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(21979..22812)
                     /locus_tag="ACG8KQ_001227"
     CDS             complement(21979..22812)
                     /locus_tag="ACG8KQ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104170.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22805..23722)
                     /locus_tag="ACG8KQ_001228"
     CDS             complement(22805..23722)
                     /locus_tag="ACG8KQ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104171.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23949..25073)
                     /locus_tag="ACG8KQ_001229"
     CDS             complement(23949..25073)
                     /locus_tag="ACG8KQ_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9104172.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7738 a         5016 c         4285 g         8070 t
ORIGIN      
        1 ctctattact catataaata aaatttatta tacctgctct ttttgactat atcctcatct
       61 gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt acagggctaa
      121 acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa
      181 aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa caatctgtcc
      241 aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc ttcttaatga
      301 atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat ttagttgaat
      361 tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac aacgaaattt
      421 tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat atcaaagcaa
      481 gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca gatgcagaag
      541 gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc ggtaaattga
      601 tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta tcaatcgacg
      661 ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat cgtcatgctc
      721 gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca gctactgaat
      781 tattcccaga attgttatta cattgggaag attttggacg aggaaatgct gcaacgatct
      841 tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc acaggaatcg
      901 ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg aaagatcaaa
      961 cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc cttttagatg
     1021 aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat caagtagata
     1081 aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc ccattcgctc
     1141 gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca gttgtaaaag
     1201 caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc actgaagaaa
     1261 tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg tctaatccaa
     1321 caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga aaagctttag
     1381 ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac caaatcggtc
     1441 aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct tcaacagcta
     1501 cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga atcgttgatg
     1561 taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa ttctctcaaa
     1621 cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt gaagaaatca
     1681 cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa tctttagaag
     1741 attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta aaaagaaaaa
     1801 agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt ctctttggct
     1861 tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa acaacaaccg
     1921 caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt accggcgtaa
     1981 gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct ttaggaaaat
     2041 taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat ttatttcatt
     2101 ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc gtttttgcga
     2161 tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta gtaaaatcca
     2221 cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc ttgattactg
     2281 gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt tttattcccg
     2341 ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt atgcttatcg
     2401 gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct ggtggtgttg
     2461 gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg gacgcttctt
     2521 ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct atcattggtg
     2581 cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat ggtaaattaa
     2641 tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc attccacaat
     2701 taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc tgtaactatt
     2761 ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc tgtaaaatcg
     2821 caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac ttgattgtca
     2881 aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat ctaaatgtat
     2941 tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt atcgcgattt
     3001 cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa tcaactatta
     3061 ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc ttatctgcag
     3121 ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt ggtgccatcg
     3181 ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa atacctgatc
     3241 atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga ttttcaaaaa
     3301 tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt gtgcttctac
     3361 tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg cttcttctac
     3421 gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca cagcaagtgc
     3481 cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt gtacatagcc
     3541 agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt ctggctcctt
     3601 caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc ctaattgttc
     3661 cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca acatccaaat
     3721 caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact ccttttttca
     3781 cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt ctaatgtttg
     3841 caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa tcgcttgaaa
     3901 aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc acaaacctcc
     3961 aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca taaagctact
     4021 tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag atagagaata
     4081 tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa cattgactgg
     4141 tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac atataaggta
     4201 ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga agaacgcaaa
     4261 aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct cgttttcaaa
     4321 taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa cagctccact
     4381 agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt gtcgcacagt
     4441 cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc ctgcaacaga
     4501 atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca tcccggaaat
     4561 agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa tcatcttgta
     4621 tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta ttatacagta
     4681 aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta gtgctaaacg
     4741 aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat cgattctttt
     4801 gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat cccattctgc
     4861 gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga tgtatgcata
     4921 agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa gaacatagtt
     4981 acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg tttcatttcc
     5041 ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat ggttatgttc
     5101 tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct gagccgtcta
     5161 aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt cgtttcgctc
     5221 ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt agtcgttcat
     5281 cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat gacatggaag
     5341 cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact acaaaacggt
     5401 ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct tcttctgcct
     5461 cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg ctcacagcaa
     5521 cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca ctccatgatt
     5581 tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac ggcgaatcaa
     5641 atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca agtagccgac
     5701 aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa tcgctaatgt
     5761 ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa
     5821 acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct acacccttat
     5881 tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat aaattaagaa
     5941 acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct ctttccataa
     6001 agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata aagttcataa
     6061 aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc tcataattga
     6121 tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt ttgtcttcaa
     6181 tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg aaatgaggag
     6241 tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata tcattttcgt
     6301 gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt ttaatgttat
     6361 tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac cattgtccgt
     6421 cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct ctcttttcag
     6481 taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc aatttgtcta
     6541 tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt gtaaactccg
     6601 gctgattccc attgatttct atttcttgat gttttccatc atattctggc acatccgcta
     6661 cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag atatctgaat
     6721 ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg atccccacga
     6781 tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc cctaaatctt
     6841 aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa aaaataaaac
     6901 cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct ctactcaatc
     6961 acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta atactcaaaa
     7021 ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct tattacttga
     7141 tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa cccgcgacat
     7201 cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa acagtatacg
     7261 cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg ttttgtagta
     7321 aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag aattagatct
     7381 gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg cggagatgta
     7441 tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc gtttggtcag
     7501 ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca tccgaatgga
     7561 acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg atctttcacg
     7621 taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc aagcaatcga
     7681 ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg
     7741 aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt tagtcattgg
     7801 aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca aatttttatc
     7861 ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac gttttggctt
     7921 aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc ctggagttca
     7981 aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag tttctcgcgg
     8041 aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta ttttccagct
     8101 tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt cttttagttt
     8161 tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat tgatttgttg
     8221 tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa taggcggatc
     8281 tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg atcttttctc
     8341 taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc ctctagtccc
     8401 tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc gagaagcggc
     8461 tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc ttgtattatc
     8521 agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa
     8581 acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac
     8641 gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa ccgcaggagc
     8701 aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct cacattttta
     8761 cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct gtaaaaaagg
     8821 ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt ccaaaacgtt
     8881 cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat cacaggaata
     8941 ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat aagaataata
     9001 ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga acctctgtta
     9061 aataattggt taatattggt ccagttcagc agcagcaacc gataatctct agcgaaataa
     9121 cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg gataaaaaac
     9181 aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa aaaactctgc
     9241 aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat tggcaaagaa
     9301 cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa agaagtctga
     9361 ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat taacttatta
     9421 tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa aatgattgga
     9481 gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac taattgagca
     9541 ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct ttttattttg
     9601 aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag ctgttttaca
     9661 gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa accttgttga
     9721 gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg atgccgatct
     9781 gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc agcaaatgcg
     9841 atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt tttaaacact
     9901 gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat
     9961 agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc agctcgccat
    10021 ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt caaagcaact
    10081 accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa aaatacttgc
    10141 aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt taaacctaac
    10201 ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa gttcatagca
    10261 aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa
    10321 agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt cgtgagctgt
    10381 ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt atacatacgg
    10441 tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta gcgtttcatc
    10501 aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac ctttcttcaa
    10561 aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag aaaaaatgac
    10621 tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg ctgcttgagg
    10681 atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca gcaacgtgcc
    10741 gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt cattttgatg
    10801 actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc
    10861 taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt gtaaaaaaat
    10921 atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa
    10981 tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa ctaaactttt
    11041 aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt ccccttgtct
    11101 tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac ttacattgat
    11161 gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta cccttcgtta
    11221 ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg actaggctgc
    11281 tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat atgagtcttt
    11341 gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt agtttatcaa
    11401 tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt tcgtttactc
    11461 tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct ttacataaat
    11521 actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga atctcttcat
    11581 ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga taaaggattt
    11641 catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac ctttttttcg
    11701 tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg atgctttttt
    11761 tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc tttcgattaa
    11821 taacagttat tgctttattc ggatgagacg ttagataaac aattttacac ttttcattct
    11881 tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa tcgatgtcta
    11941 ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa
    12001 cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca
    12061 aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta
    12121 ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa
    12181 catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc
    12241 gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca
    12301 tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt
    12361 attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca
    12421 gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg
    12481 atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc
    12541 gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag
    12601 aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt
    12661 ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa atagttttca
    12721 tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct
    12781 aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc
    12841 tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc
    12901 gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt
    12961 acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt
    13021 gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga
    13081 gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg
    13141 agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac
    13201 cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag
    13261 tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt
    13321 gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact
    13381 taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa
    13441 taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt
    13501 tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt
    13561 atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt
    13621 ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc
    13681 tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac
    13741 accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga
    13801 attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag
    13861 cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg
    13921 tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga
    13981 gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa
    14041 ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa
    14101 gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct
    14161 ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc
    14221 tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt
    14281 tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa
    14341 tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg
    14401 tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg
    14461 aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag
    14521 tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag
    14581 aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac
    14641 tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt
    14701 ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt
    14761 ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat
    14821 caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt
    14881 caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac
    14941 tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt
    15001 gatttgttct gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac
    15061 gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt
    15121 gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg
    15181 atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc
    15241 acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg
    15301 aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct
    15361 taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt
    15421 cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat
    15481 ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt
    15541 atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa
    15601 aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta
    15661 caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta
    15721 gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact
    15781 ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa
    15841 acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg
    15901 aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta
    15961 gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa
    16021 tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt
    16081 cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact
    16141 ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac
    16201 attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag
    16261 aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta
    16321 gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt
    16381 aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat
    16441 gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc
    16501 gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg
    16561 gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct
    16621 ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct
    16681 gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt
    16741 atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa
    16801 gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat
    16861 acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag
    16921 atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct
    16981 ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa
    17041 aattttttga ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta
    17101 gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc
    17161 atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta
    17221 ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc cgaattgttg
    17281 gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta ttaccaaagc
    17341 aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa
    17401 atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc gccagaaaac
    17461 ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc tagacaagat
    17521 caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga atatgaaaat
    17581 taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt ttttatttta
    17641 tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta ctagtcattc
    17701 tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt accccattat
    17761 ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc gttgtagcat
    17821 atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag ttcttttctg
    17881 ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg atcaccatac
    17941 tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa taacaaatgg
    18001 tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa tcaaaatatt
    18061 tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat gcttggcctc
    18121 tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt ggttttccat
    18181 ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact aaataatctg
    18241 ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca gtttcttcac
    18301 tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta ccttgttgtg
    18361 aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa aaacgctgaa
    18421 gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc gttattttat
    18481 aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta gtgataattt
    18541 gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt tgaaataaag
    18601 gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct tctgtccaac
    18661 caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg aattgatact
    18721 gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc tcgatctcat
    18781 tattccattc tttttccatt aattctttat aacattcatt tatccatttt tctggatgta
    18841 ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc ataattttag
    18901 tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa aatcaatttt
    18961 gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa ccactcctgt
    19021 tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa aagtgttttc
    19081 ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc gtttaatagt
    19141 atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat agttttcctt
    19201 gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa accctgtctc
    19261 atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc agccttcaaa
    19321 aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag aggcaagtac
    19381 ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc cataggtttt
    19441 gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat
    19501 tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg
    19561 actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat ttggccctaa
    19621 ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca aattggcttc
    19681 tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg tccaatactc
    19741 atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg gagataacgt
    19801 actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat tcaggatacc
    19861 tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga ctaacccctt
    19921 gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc aaaagcttga
    19981 aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa
    20041 aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct ggctaatggg
    20101 cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa aaggattata
    20161 ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat aacaattttc
    20221 tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa atttttggat
    20281 aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt catattcaaa
    20341 aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt ttttccataa
    20401 taattgttca tgatgatata acgctaataa cattgcgggc atttctacca tacgctggtc
    20461 tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag cttttattcc
    20521 tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc ccgctcccca
    20581 taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta ccccttgatg
    20641 tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt gatttatctt
    20701 tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat gtttcattgt
    20761 attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg aagtaagcct
    20821 tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc tctatggtat
    20881 gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc atagtgcttt
    20941 tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg tacagaggaa
    21001 acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact ggtatcaata
    21061 ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc gattttctga
    21121 ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt tgtgtaacga
    21181 taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca agaattctca
    21241 ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc aaattctgcg
    21301 atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc tgcatgctgc
    21361 cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata cagtaatggt
    21421 aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca tgctactaaa
    21481 tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt
    21541 cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa
    21601 cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa ttgttcttgt
    21661 tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg ccataaaata
    21721 ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac gtctgtatat
    21781 tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc cgcatctatc
    21841 ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc attcatggat
    21901 atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga actagatggt
    21961 taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct taaatttaga
    22021 ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa ttagcataat
    22081 tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga taattaaggg
    22141 aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt gattccaact
    22201 tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg gtacagcgat
    22261 tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca tcagtgctgt
    22321 aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg caatcgaagg
    22381 gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt aaaataaagg
    22441 aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga acaacagttt
    22501 attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac tggctgtagc
    22561 tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg gataattata
    22621 tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga aattgattcc
    22681 gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata aatatggcat
    22741 caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc tttgtatctt
    22801 ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat ataacagata
    22861 caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt tcaaaacttg
    22921 ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg gaagtcccaa
    22981 agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa actgtggcca
    23041 ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga aatttcgatg
    23101 ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga ccagctaaaa
    23161 ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt gatattcgta
    23221 tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc aaaaatccat
    23281 tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc accgttgatg
    23341 taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac tgtgctgaat
    23401 tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat cctgccatga
    23461 taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata gctagataat
    23521 tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa gcaaacgaga
    23581 taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg accaatccat
    23641 aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt attgtcttgc
    23701 ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc accagttaat
    23761 tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt ttcaaaatca
    23821 cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc tgaataagtg
    23881 gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc atagtatttt
    23941 tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc caatgagtta
    24001 gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt cagcataaat
    24061 gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata aaaacttgat
    24121 cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag tggagccatg
    24181 cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata ccagccgcca
    24241 gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt
    24301 acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc ttctttagta
    24361 ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc tagcatgttg
    24421 ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc agttttttct
    24481 tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc aaaatctaca
    24541 cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa atcactagcc
    24601 caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt ttgatcataa
    24661 aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa agcatcttga
    24721 aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc cggcatatct
    24781 tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga agctacattc
    24841 acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc agcattttca
    24901 acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc tttttttatc
    24961 attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc acttaagctg
    25021 aataataggg taatgtttag caaagctatc accaatttgt atcttttttt catgtctatc
    25081 ccttctttct tttcaagttc ttatataaa
//
LOCUS       DAZKCE010000035        24416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000035 DAZKCE010000000
VERSION     DAZKCE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.035"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..937)
                     /locus_tag="ACG8KQ_001230"
     CDS             complement(89..937)
                     /locus_tag="ACG8KQ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9104173.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1161..3101)
                     /gene="thrS"
                     /locus_tag="ACG8KQ_001231"
     CDS             complement(1161..3101)
                     /gene="thrS"
                     /locus_tag="ACG8KQ_001231"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9104174.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3438..3617)
                     /locus_tag="ACG8KQ_001232"
     CDS             complement(3438..3617)
                     /locus_tag="ACG8KQ_001232"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9104175.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3664..5487)
                     /locus_tag="ACG8KQ_001233"
     CDS             complement(3664..5487)
                     /locus_tag="ACG8KQ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9104176.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5765..8044
                     /gene="helD"
                     /locus_tag="ACG8KQ_001234"
     CDS             5765..8044
                     /gene="helD"
                     /locus_tag="ACG8KQ_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9104177.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8137..8784)
                     /locus_tag="ACG8KQ_001235"
     CDS             complement(8137..8784)
                     /locus_tag="ACG8KQ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104178.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8963..9682)
                     /locus_tag="ACG8KQ_001236"
     CDS             complement(8963..9682)
                     /locus_tag="ACG8KQ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9104179.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9813..10964)
                     /locus_tag="ACG8KQ_001237"
     CDS             complement(9813..10964)
                     /locus_tag="ACG8KQ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9104180.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11181..11921)
                     /locus_tag="ACG8KQ_001238"
     CDS             complement(11181..11921)
                     /locus_tag="ACG8KQ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9104181.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11925..12248)
                     /gene="rsfS"
                     /locus_tag="ACG8KQ_001239"
     CDS             complement(11925..12248)
                     /gene="rsfS"
                     /locus_tag="ACG8KQ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9104182.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12305..12898)
                     /gene="yqeK"
                     /locus_tag="ACG8KQ_001240"
     CDS             complement(12305..12898)
                     /gene="yqeK"
                     /locus_tag="ACG8KQ_001240"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9104183.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12882..13532)
                     /locus_tag="ACG8KQ_001241"
     CDS             complement(12882..13532)
                     /locus_tag="ACG8KQ_001241"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9104184.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13542..13853)
                     /gene="yhbY"
                     /locus_tag="ACG8KQ_001242"
     CDS             complement(13542..13853)
                     /gene="yhbY"
                     /locus_tag="ACG8KQ_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9104185.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13869..14978)
                     /gene="yqeH"
                     /locus_tag="ACG8KQ_001243"
     CDS             complement(13869..14978)
                     /gene="yqeH"
                     /locus_tag="ACG8KQ_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9104186.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14975..15502)
                     /locus_tag="ACG8KQ_001244"
     CDS             complement(14975..15502)
                     /locus_tag="ACG8KQ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9104187.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15682..16467)
                     /locus_tag="ACG8KQ_001245"
     CDS             complement(15682..16467)
                     /locus_tag="ACG8KQ_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9104188.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16460..17329)
                     /gene="accD"
                     /locus_tag="ACG8KQ_001246"
     CDS             complement(16460..17329)
                     /gene="accD"
                     /locus_tag="ACG8KQ_001246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9104189.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17330..18709)
                     /locus_tag="ACG8KQ_001247"
     CDS             complement(17330..18709)
                     /locus_tag="ACG8KQ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9104190.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18717..19136)
                     /gene="fabZ"
                     /locus_tag="ACG8KQ_001248"
     CDS             complement(18717..19136)
                     /gene="fabZ"
                     /locus_tag="ACG8KQ_001248"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9104191.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19161..19637)
                     /gene="accB"
                     /locus_tag="ACG8KQ_001249"
     CDS             complement(19161..19637)
                     /gene="accB"
                     /locus_tag="ACG8KQ_001249"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9104192.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19640..20875)
                     /gene="fabF"
                     /locus_tag="ACG8KQ_001250"
     CDS             complement(19640..20875)
                     /gene="fabF"
                     /locus_tag="ACG8KQ_001250"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9104193.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20894..21631)
                     /gene="fabG"
                     /locus_tag="ACG8KQ_001251"
     CDS             complement(20894..21631)
                     /gene="fabG"
                     /locus_tag="ACG8KQ_001251"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9104194.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21643..22560)
                     /gene="fabD"
                     /locus_tag="ACG8KQ_001252"
     CDS             complement(21643..22560)
                     /gene="fabD"
                     /locus_tag="ACG8KQ_001252"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9104195.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22667..22891)
                     /locus_tag="ACG8KQ_001253"
     CDS             complement(22667..22891)
                     /locus_tag="ACG8KQ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9104196.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22944..23906)
                     /locus_tag="ACG8KQ_001254"
     CDS             complement(22944..23906)
                     /locus_tag="ACG8KQ_001254"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9104197.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23916..24356)
                     /locus_tag="ACG8KQ_001255"
     CDS             complement(23916..24356)
                     /locus_tag="ACG8KQ_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9104198.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6681 a         5432 c         4256 g         8047 t
ORIGIN      
        1 aagtcgtgaa acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa
       61 aaaacttata taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg
      121 cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa
      181 aaatgatatc gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg
      241 tgatttcttt tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat
      301 ttccaaattg cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg
      361 cgactacatc tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat
      421 tgctgacaag ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga
      481 cacgagacca tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata
      541 aaaaaacagt ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta
      601 gaatatctac tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag
      661 agtaaaagga ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt
      721 ctgttgaaat cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac
      781 gtacaactgt cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag
      841 ggaccttctc aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat
      901 cttccatcat actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga
      961 tagtctatgt aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac
     1021 aaaaaaacaa tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc
     1081 atctgtgtct ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt
     1141 ttttacagga aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata
     1201 gcatcaataa agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg
     1261 ttcactgttg catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt
     1321 gtttgagagg cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc
     1381 aaccccttca tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca
     1441 gaaacaggga tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat
     1501 acttctgtta aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact
     1561 actggacgat gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc
     1621 aataagaagt ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact
     1681 tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt
     1741 tctaattcat ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca
     1801 tcaaaatatt tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta
     1861 atattgaagt cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg
     1921 atttgatcag gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt
     1981 aaacctgaca aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga
     2041 attggtaatt cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag
     2101 ttcattgggc gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct
     2161 tcttgataat gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt
     2221 gtatatacat gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg
     2281 cgtcggattg ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa
     2341 accatgaaca agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca
     2401 cggagacgga tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt
     2461 tgcatcattt gattgtttga gtttccacgc caataagcac ctgccactga taataattta
     2521 aatacttgta tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg
     2581 ccttgttgat aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg
     2641 tacggatcac ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc
     2701 ggcagatttt ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca
     2761 gttacttggt tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca
     2821 acgccaaagt ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct
     2881 gaatgacgga ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca
     2941 tcttcctcga ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct
     3001 ttttttgcta agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca
     3061 aattctttga ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa
     3121 tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa
     3181 gagatactag gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc
     3241 aagcgttgta acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt
     3301 tggttttctc ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat
     3361 tcagttggct ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat
     3421 caagtgggaa actaaactta ttttttccat tccccattta caccgtatgt tccttttttc
     3481 atctcattga tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct
     3541 tccgtgatat ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg
     3601 acatgtacga atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt
     3661 ctatcatact atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc
     3721 tctcaaggca ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa
     3781 tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat
     3841 cagtacagac tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac
     3901 acgcaagaga tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc
     3961 atgagccccc attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc
     4021 gtctcctacc atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt
     4081 atcttgaggc aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg
     4141 agcaatggca gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag
     4201 ctccttgatt gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc
     4261 aaaagatcga tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga
     4321 aacaaaagag gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg
     4381 accggtcact tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac
     4441 taacgaacga gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag
     4501 ctcttctttt tttattccat tgactggctg aatcgcctct acttccaata tccctttggt
     4561 aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt
     4621 tccagttttt acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat
     4681 gggcgcagct agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa
     4741 agggtctttt gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg
     4801 gactgcataa cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac
     4861 tagtttaata atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat
     4921 cgatccgtct ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg
     4981 gcgggactca cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc
     5041 atctgcgggt acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc
     5101 gattactata tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc
     5161 taacaaagac tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc
     5221 gcccgtaagc atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc
     5281 aaggattgcg gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat
     5341 catttccaaa aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc
     5401 aagggccggc tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt
     5461 aagtaaaaat tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag
     5521 gttagtaata aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt
     5581 ttaaaacaat aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca
     5641 gttctacaaa caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac
     5701 cttttttcga aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca
     5761 tgcgatgaat gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca
     5821 gatcgaacaa aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc
     5881 gacaaaagac acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc
     5941 tgctgttgaa tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc
     6001 acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat
     6061 cgactttaaa gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga
     6121 tctggcagaa gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga
     6181 aggtgagatt ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca
     6241 gttgaaacga caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga
     6301 agtaatcaat gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa
     6361 aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa
     6421 agtaatgctg attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat
     6481 tgcttttctg ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc
     6541 acccaaccat ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg
     6601 cgtgcctaca cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact
     6661 agatgaacag caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat
     6721 gaaaagcgga ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta
     6781 cggtcccttg ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat
     6841 tcgccaatgg tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt
     6901 acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg
     6961 ggtcaaagat ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga
     7021 atatacagaa caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa
     7081 aagattccgt cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca
     7141 atatctccac tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga
     7201 gaccaggtgg caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg
     7261 tcaagaggat gctgtactat tctttttact gatgcgccga acatatccag tgacggttac
     7321 acacaaagct cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc
     7381 attgctgcaa gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa
     7441 tgtctttgga aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt
     7501 cactcgtttc caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa
     7561 tcattttatt gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt
     7621 gctcatccaa ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac
     7681 gactcaggga aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg
     7741 cgaagctctt tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa
     7801 tgagagtgac tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct
     7861 ggagtttgac cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga
     7921 caaattgatc ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc
     7981 caataatgaa agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact
     8041 gtaagtaaaa agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat
     8101 aattgcagaa aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc
     8161 tgtttcagga tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc
     8221 tggatattct gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg
     8281 gtaaattcga ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc
     8341 gccacaatcc aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt
     8401 ttttttcgtg tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa
     8461 gctccactct aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa
     8521 ggctgaatca gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat
     8581 tggtgcgcgg tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt
     8641 tcctaatcga tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc
     8701 agttacgttc acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg
     8761 ttctaaagtg atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca
     8821 tttcacatga tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc
     8881 acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat
     8941 acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta
     9001 catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag
     9061 atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc
     9121 ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga
     9181 tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa
     9241 ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag
     9301 aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg
     9361 ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc
     9421 caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga
     9481 ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac
     9541 gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa
     9601 tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga
     9661 tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg
     9721 caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc
     9781 ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa
     9841 ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc
     9901 gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt
     9961 aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg
    10021 acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt
    10081 gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt
    10141 gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt
    10201 tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc
    10261 tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt
    10321 tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg
    10381 attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca
    10441 tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca
    10501 agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac
    10561 gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag
    10621 cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca
    10681 cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc
    10741 gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg
    10801 cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca
    10861 gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca
    10921 ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat
    10981 agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc
    11041 cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa
    11101 gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt
    11161 aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc
    11221 tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt
    11281 tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca
    11341 cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg
    11401 ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa
    11461 tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa
    11521 aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt
    11581 ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt
    11641 tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct
    11701 cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca
    11761 tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt
    11821 gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc
    11881 atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca
    11941 ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct
    12001 ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct
    12061 tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct
    12121 tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc
    12181 aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct
    12241 gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag
    12301 cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt
    12361 gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta
    12421 gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa
    12481 tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat
    12541 gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg
    12601 aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac
    12661 cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg
    12721 tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg
    12781 ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt
    12841 tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc atattcatac
    12901 aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag
    12961 ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc
    13021 caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg
    13081 accatagaag tcagctcatc gattttatac cattttggca aatattctac catatcccca
    13141 ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca
    13201 taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg
    13261 tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca
    13321 tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg
    13381 ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag
    13441 atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa
    13501 cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct
    13561 tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca
    13621 atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca
    13681 atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct
    13741 tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga
    13801 agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc
    13861 cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc
    13921 gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt
    13981 ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga
    14041 cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt
    14101 ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg
    14161 ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga
    14221 taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag
    14281 tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca
    14341 tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca
    14401 tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt
    14461 gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt
    14521 tccgtcatct catgagattt cttagcacta gttaataata catctactgg acgaattcct
    14581 gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt
    14641 aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt
    14701 cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct
    14761 ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg
    14821 gtctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa
    14881 cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata
    14941 acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca
    15001 tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt
    15061 gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac
    15121 gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg
    15181 ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg
    15241 caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg
    15301 aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt
    15361 ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt
    15421 tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct accatccatg
    15481 ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa
    15541 agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt
    15601 tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa
    15661 aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa
    15721 tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat
    15781 gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc
    15841 aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc
    15901 ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata
    15961 aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact
    16021 tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag
    16081 atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc
    16141 tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg
    16201 taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg
    16261 cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat
    16321 tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga
    16381 atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg
    16441 aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa
    16501 gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat
    16561 gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt
    16621 cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc
    16681 cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat
    16741 aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta
    16801 gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa
    16861 gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca
    16921 tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg
    16981 ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt
    17041 tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt
    17101 caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt
    17161 atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc
    17221 aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct
    17281 ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc
    17341 ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt
    17401 aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga
    17461 tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac
    17521 gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat
    17581 atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt
    17641 tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc
    17701 attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg
    17761 ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat
    17821 gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca
    17881 gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt
    17941 ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt
    18001 tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc
    18061 gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca
    18121 tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga
    18181 gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca
    18241 gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata
    18301 caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga
    18361 tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact
    18421 cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg
    18481 cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa
    18541 cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg
    18601 catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga
    18661 tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat
    18721 ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct
    18781 ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct
    18841 cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt
    18901 cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca
    18961 atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg
    19021 atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca
    19081 atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt
    19141 ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg
    19201 acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc
    19261 tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca
    19321 cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt
    19381 ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga
    19441 gcagttgttg cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt
    19501 gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga
    19561 ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg
    19621 atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc
    19681 cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt
    19741 tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa
    19801 attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata
    19861 aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg
    19921 cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat
    19981 tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt
    20041 tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac
    20101 cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt
    20161 caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg
    20221 gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag
    20281 caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc
    20341 catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta
    20401 cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt
    20461 tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac
    20521 gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa
    20581 accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag
    20641 cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt
    20701 tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa
    20761 atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg
    20821 cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt
    20881 tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg
    20941 tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga
    21001 aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg
    21061 tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag
    21121 cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg
    21181 cttgaccaat atttccaatc aacccagata cactagagag attaatgatg gccccttctc
    21241 gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga
    21301 tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga
    21361 ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca
    21421 tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga
    21481 atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat
    21541 ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg
    21601 tgataaatac attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt
    21661 ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt
    21721 cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt
    21781 ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag
    21841 tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga
    21901 aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa
    21961 aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc
    22021 ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg
    22081 aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc
    22141 ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc
    22201 agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag
    22261 cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg
    22321 gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg
    22381 ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc
    22441 gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa
    22501 atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat
    22561 gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac
    22621 attcctattt caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt
    22681 tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca
    22741 cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta
    22801 agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg
    22861 cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct
    22921 ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct
    22981 ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa
    23041 actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata tttatccata
    23101 ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc aataattctg
    23161 aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata
    23221 ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa
    23281 tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc
    23341 aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga
    23401 ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca
    23461 gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg
    23521 cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg
    23581 atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat
    23641 ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt
    23701 agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga
    23761 tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta
    23821 tccatcatct tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt
    23881 gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg
    23941 tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag
    24001 atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt
    24061 ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag
    24121 ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct
    24181 ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc
    24241 tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa
    24301 atatcattga agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc
    24361 caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat atctta
//
LOCUS       DAZKCE010000036        24249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000036 DAZKCE010000000
VERSION     DAZKCE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.036"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8KQ_001256"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8KQ_001256"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9104199.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8KQ_001257"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8KQ_001257"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9104200.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8KQ_001258"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8KQ_001258"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP9104201.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8KQ_001259"
     CDS             2925..3533
                     /locus_tag="ACG8KQ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9104202.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8KQ_001260"
     CDS             3858..4181
                     /locus_tag="ACG8KQ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9104203.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8KQ_001261"
     CDS             4363..5733
                     /locus_tag="ACG8KQ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9104204.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8KQ_001262"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8KQ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9104205.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8KQ_001263"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8KQ_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9104206.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8KQ_001264"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8KQ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9104207.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8KQ_001265"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8KQ_001265"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9104208.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8KQ_001266"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8KQ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9104209.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8KQ_001267"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8KQ_001267"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9104210.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8KQ_001268"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8KQ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9104211.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8KQ_001269"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8KQ_001269"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9104212.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8KQ_001270"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8KQ_001270"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9104213.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8KQ_001271"
     CDS             16339..16635
                     /locus_tag="ACG8KQ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104214.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8KQ_001272"
     CDS             17203..18795
                     /locus_tag="ACG8KQ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9104215.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8KQ_001273"
     CDS             18808..19548
                     /locus_tag="ACG8KQ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9104216.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8KQ_001274"
     CDS             19716..21065
                     /locus_tag="ACG8KQ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9104217.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8KQ_001275"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8KQ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9104218.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8KQ_001276"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8KQ_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9104219.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            23753..>24249
                     /locus_tag="ACG8KQ_001277"
     CDS             23753..>24249
                     /locus_tag="ACG8KQ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP9104220.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         6999 a         4868 c         4601 g         7781 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aacttgagag tataaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaagtccc
    23701 ttctgtagaa taaagttaac gacaaccaat tcacagaaaa gaggacttcc ctatgaatga
    23761 ttttactaca gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg
    23821 ttcgcactta gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga
    23881 ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt
    23941 tcgatcaatc aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg
    24001 tgagtttaaa caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac
    24061 tattatccat ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa
    24121 aatgtacggt catcactata ctccacaaac catgtccaac atgactaaag ttctgactga
    24181 agaagtaaat gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga
    24241 cgctactta
//
LOCUS       DAZKCE010000037        24097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000037 DAZKCE010000000
VERSION     DAZKCE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.037"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8KQ_001278"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8KQ_001278"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9104221.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8KQ_001279"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8KQ_001279"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9104222.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8KQ_001280"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8KQ_001280"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9104223.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG8KQ_001281"
     CDS             complement(3374..4681)
                     /locus_tag="ACG8KQ_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9104224.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG8KQ_001282"
     CDS             complement(4684..5265)
                     /locus_tag="ACG8KQ_001282"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9104225.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG8KQ_001283"
     CDS             complement(5837..7129)
                     /locus_tag="ACG8KQ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9104226.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG8KQ_001284"
     CDS             complement(7126..7749)
                     /locus_tag="ACG8KQ_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104227.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG8KQ_001285"
     CDS             complement(7752..8588)
                     /locus_tag="ACG8KQ_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104228.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG8KQ_001286"
     CDS             complement(8605..9492)
                     /locus_tag="ACG8KQ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104229.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG8KQ_001287"
     CDS             complement(9508..10791)
                     /locus_tag="ACG8KQ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9104230.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG8KQ_001288"
     CDS             complement(11474..12157)
                     /locus_tag="ACG8KQ_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9104231.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG8KQ_001289"
     CDS             complement(12175..12792)
                     /locus_tag="ACG8KQ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9104232.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG8KQ_001290"
     CDS             complement(12802..13626)
                     /locus_tag="ACG8KQ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9104233.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG8KQ_001291"
     CDS             complement(13619..14179)
                     /locus_tag="ACG8KQ_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104234.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG8KQ_001292"
     CDS             14353..15309
                     /locus_tag="ACG8KQ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9104235.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG8KQ_001293"
     CDS             complement(15614..16570)
                     /locus_tag="ACG8KQ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9104236.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG8KQ_001294"
     CDS             16674..17276
                     /locus_tag="ACG8KQ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9104237.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG8KQ_001295"
     CDS             complement(17364..18863)
                     /locus_tag="ACG8KQ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9104238.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG8KQ_001296"
     CDS             complement(18879..19403)
                     /locus_tag="ACG8KQ_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9104239.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG8KQ_001297"
     CDS             19701..20450
                     /locus_tag="ACG8KQ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9104240.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG8KQ_001298"
     CDS             20441..21226
                     /locus_tag="ACG8KQ_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9104241.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG8KQ_001299"
     CDS             21195..21911
                     /locus_tag="ACG8KQ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9104242.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG8KQ_001300"
     CDS             complement(22013..23032)
                     /locus_tag="ACG8KQ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP9104243.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..>24097)
                     /locus_tag="ACG8KQ_001301"
     CDS             complement(23051..>24097)
                     /locus_tag="ACG8KQ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9104244.1"
                     /translation="DIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILS
                     KVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQ
                     TTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEA
                     KNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQS
                     MDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTA
                     TNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVW
                     IVVLQMIHGIGLFS"
BASE COUNT         7131 a         5202 c         4210 g         7554 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtc
//
LOCUS       DAZKCE010000038        23954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000038 DAZKCE010000000
VERSION     DAZKCE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.038"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..331)
                     /locus_tag="ACG8KQ_001302"
     CDS             complement(2..331)
                     /locus_tag="ACG8KQ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9104245.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            508..1236
                     /gene="sfsA"
                     /locus_tag="ACG8KQ_001303"
     CDS             508..1236
                     /gene="sfsA"
                     /locus_tag="ACG8KQ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9104246.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1252..2265
                     /locus_tag="ACG8KQ_001304"
     CDS             1252..2265
                     /locus_tag="ACG8KQ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9104247.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2265..3542
                     /locus_tag="ACG8KQ_001305"
     CDS             2265..3542
                     /locus_tag="ACG8KQ_001305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9104248.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3605..6307
                     /locus_tag="ACG8KQ_001306"
     CDS             3605..6307
                     /locus_tag="ACG8KQ_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9104249.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6459..6776
                     /locus_tag="ACG8KQ_001307"
     CDS             6459..6776
                     /locus_tag="ACG8KQ_001307"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9104250.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6806..7126
                     /locus_tag="ACG8KQ_001308"
     CDS             6806..7126
                     /locus_tag="ACG8KQ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9104251.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7234..8694
                     /locus_tag="ACG8KQ_001309"
     CDS             7234..8694
                     /locus_tag="ACG8KQ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9104252.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8762..8983
                     /locus_tag="ACG8KQ_001310"
     CDS             8762..8983
                     /locus_tag="ACG8KQ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104253.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9014..9196
                     /locus_tag="ACG8KQ_001311"
     CDS             9014..9196
                     /locus_tag="ACG8KQ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9104254.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9196..9609
                     /locus_tag="ACG8KQ_001312"
     CDS             9196..9609
                     /locus_tag="ACG8KQ_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9104255.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9732..10913
                     /locus_tag="ACG8KQ_001313"
     CDS             9732..10913
                     /locus_tag="ACG8KQ_001313"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104256.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10984..11148)
                     /locus_tag="ACG8KQ_001314"
     CDS             complement(10984..11148)
                     /locus_tag="ACG8KQ_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104257.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11302..11375
                     /locus_tag="ACG8KQ_001315"
     tRNA            11302..11375
                     /locus_tag="ACG8KQ_001315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11336..11338,aa:Arg,seq:cct)
     gene            complement(11444..12583)
                     /locus_tag="ACG8KQ_001316"
     CDS             complement(11444..12583)
                     /locus_tag="ACG8KQ_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9104258.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            12755..12889
                     /locus_tag="ACG8KQ_001317"
     CDS             12755..12889
                     /locus_tag="ACG8KQ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104259.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(12882..13517)
                     /locus_tag="ACG8KQ_001318"
     CDS             complement(12882..13517)
                     /locus_tag="ACG8KQ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104260.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13630..14265)
                     /locus_tag="ACG8KQ_001319"
     CDS             complement(13630..14265)
                     /locus_tag="ACG8KQ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFP9104261.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14299..14760)
                     /locus_tag="ACG8KQ_001320"
     CDS             complement(14299..14760)
                     /locus_tag="ACG8KQ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFP9104262.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14890..15321)
                     /locus_tag="ACG8KQ_001321"
     CDS             complement(14890..15321)
                     /locus_tag="ACG8KQ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9104263.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15339..15659)
                     /locus_tag="ACG8KQ_001322"
     CDS             complement(15339..15659)
                     /locus_tag="ACG8KQ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9104264.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            15958..16734
                     /locus_tag="ACG8KQ_001323"
     CDS             15958..16734
                     /locus_tag="ACG8KQ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9104265.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            16749..16952
                     /locus_tag="ACG8KQ_001324"
     CDS             16749..16952
                     /locus_tag="ACG8KQ_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104266.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            16968..17306
                     /locus_tag="ACG8KQ_001325"
     CDS             16968..17306
                     /locus_tag="ACG8KQ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP9104267.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            17293..17472
                     /locus_tag="ACG8KQ_001326"
     CDS             17293..17472
                     /locus_tag="ACG8KQ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104268.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17515..17985)
                     /locus_tag="ACG8KQ_001327"
     CDS             complement(17515..17985)
                     /locus_tag="ACG8KQ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104269.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18072..18770
                     /locus_tag="ACG8KQ_001328"
     CDS             18072..18770
                     /locus_tag="ACG8KQ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9104270.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18948..19289
                     /locus_tag="ACG8KQ_001329"
     CDS             18948..19289
                     /locus_tag="ACG8KQ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104271.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19282..19953
                     /locus_tag="ACG8KQ_001330"
     CDS             19282..19953
                     /locus_tag="ACG8KQ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9104272.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            19959..20651
                     /locus_tag="ACG8KQ_001331"
     CDS             19959..20651
                     /locus_tag="ACG8KQ_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9104273.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            20648..21397
                     /locus_tag="ACG8KQ_001332"
     CDS             20648..21397
                     /locus_tag="ACG8KQ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104274.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            21409..21678
                     /locus_tag="ACG8KQ_001333"
     CDS             21409..21678
                     /locus_tag="ACG8KQ_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104275.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            21684..21848
                     /locus_tag="ACG8KQ_001334"
     CDS             21684..21848
                     /locus_tag="ACG8KQ_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104276.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            21841..22140
                     /locus_tag="ACG8KQ_001335"
     CDS             21841..22140
                     /locus_tag="ACG8KQ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104277.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            22140..22442
                     /locus_tag="ACG8KQ_001336"
     CDS             22140..22442
                     /locus_tag="ACG8KQ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9104278.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            22482..22847
                     /locus_tag="ACG8KQ_001337"
     CDS             22482..22847
                     /locus_tag="ACG8KQ_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104279.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            23337..23477
                     /locus_tag="ACG8KQ_001338"
     CDS             23337..23477
                     /locus_tag="ACG8KQ_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104280.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            23474..23656
                     /locus_tag="ACG8KQ_001339"
     CDS             23474..23656
                     /locus_tag="ACG8KQ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104281.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            23669..23863
                     /locus_tag="ACG8KQ_001340"
     CDS             23669..23863
                     /locus_tag="ACG8KQ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104282.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         8072 a         3919 c         4732 g         7231 t
ORIGIN      
        1 tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt
       61 cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat
      121 ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg
      181 taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg
      241 agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa
      301 cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg
      361 agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta
      421 aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat
      481 acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt
      541 attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt
      601 gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct
      661 attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag
      721 aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt
      781 ttagacggca cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa
      841 gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca
      901 tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg
      961 cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat
     1021 gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa
     1081 gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc
     1141 ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt
     1201 gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac
     1261 ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca
     1321 aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt
     1381 tctcattatg aaggggatat cgaattcgca acagatttga agactttttt caatattact
     1441 catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa
     1501 gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa
     1561 atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga
     1621 aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata
     1681 atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga
     1741 gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt
     1801 taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca
     1861 agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt
     1921 gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt
     1981 gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat
     2041 cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat
     2101 gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg
     2161 tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa
     2221 aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac
     2281 aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc
     2341 ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc
     2401 ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag
     2461 gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag
     2521 atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag
     2581 ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac
     2641 caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa
     2701 tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa
     2761 ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt
     2821 cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag
     2881 aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag
     2941 atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc
     3001 aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa
     3061 gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga
     3121 aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat attacaggtc
     3181 ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg
     3241 gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc
     3301 taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat
     3361 acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata
     3421 aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa
     3481 taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat
     3541 aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg
     3601 ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt
     3661 gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt
     3721 aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc
     3781 tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac
     3841 ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc
     3901 tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg
     3961 gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc
     4021 agatgcgcca tttgtttctt tcaattgtgc ggattatgat caaaatccgc aactgctctt
     4081 tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg
     4141 tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc
     4201 ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga
     4261 aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga
     4321 aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc
     4381 ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa
     4441 tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga
     4501 tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt
     4561 tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct
     4621 tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat
     4681 ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca
     4741 agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc
     4801 cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta
     4861 tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga
     4921 tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat
     4981 ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt
     5041 gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag
     5101 ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat
     5161 tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga
     5221 gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc
     5281 aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat
     5341 gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta
     5401 tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt
     5461 cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac
     5521 catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta
     5581 tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc
     5641 tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc
     5701 tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca
     5761 gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata
     5821 tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc
     5881 agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga
     5941 gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat
     6001 tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat
     6061 tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg
     6121 attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta
     6181 tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt
     6241 aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa
     6301 aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa
     6361 agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg
     6421 ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt
     6481 tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag
     6541 aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt
     6601 tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc
     6661 aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact
     6721 atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca
     6781 agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg
     6841 gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa
     6901 aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca
     6961 agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt
     7021 gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt
     7081 agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt
     7141 ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc
     7201 tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag
     7261 aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt
     7321 gatgctttta tcggcatact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat
     7381 gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca
     7441 gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac
     7501 ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga
     7561 gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt
     7621 atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca
     7681 gatgcaatca atagttctga gattggtgca acgattgcag atggcacact gtcagttgga
     7741 ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc
     7801 atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa
     7861 atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg
     7921 atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa
     7981 ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt atctcaaggg
     8041 ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat
     8101 ggatcaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat
     8161 attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca
     8221 atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt
     8281 ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca
     8341 gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca
     8401 gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct
     8461 ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta
     8521 tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg
     8581 gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca
     8641 ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa
     8701 cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga
     8761 aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata
     8821 caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga
     8881 aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca
     8941 gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga
     9001 taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt
     9061 gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct
     9121 ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa
     9181 ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac
     9241 gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact
     9301 agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc
     9361 ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag
     9421 aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat
     9481 tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga
     9541 acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca
     9601 acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa
     9661 ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa
     9721 ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt
     9781 ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc
     9841 gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa
     9901 gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg
     9961 gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca
    10021 tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat
    10081 tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc
    10141 aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg
    10201 attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac
    10261 gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt
    10321 accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc
    10381 ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca
    10441 gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg
    10501 gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca
    10561 caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta
    10621 aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg
    10681 aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa
    10741 caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc
    10801 gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa
    10861 cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa
    10921 cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa
    10981 atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg
    11041 gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat tttttttaga
    11101 tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg gaaaggacct
    11161 ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt ggaaacgttt
    11221 tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa acaaacaagc
    11281 tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat cgcctcctaa
    11341 gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg tatcctttgc
    11401 ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat
    11461 ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
    11521 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
    11581 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
    11641 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
    11701 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttga
    11761 tttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
    11821 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
    11881 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
    11941 cccatgttaa tcctagaagt ccagcaaatc gcattccagt ggcaccagca actaacacga
    12001 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
    12061 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
    12121 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgacg
    12181 ctctaatatg gttattaaac ttaataacgg tagattttac atggtcttta gcatacttat
    12241 ttaaaaagcg ttgatatccc actctatcta aatcagaaat caataccgct ggcatatatt
    12301 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
    12361 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
    12421 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttctcctt
    12481 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
    12541 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tcagctctct
    12601 ttctctgata caataggaac tataaagaag cccattgttt gggttttatt attttttgca
    12661 catccttttt gcttgccggc gcaaggatgt gctatttgtc gttttaatgg tataattgga
    12721 tgtgtattag atcatccaat cagaggtgtt tattatgatt aaagtgattt ttaaaagaat
    12781 aactgaactt ccagagttta gattatttgt tgattattgg tatgtctggt tttttattgt
    12841 tgcattgctg ataggtctag atgtcatatt agaaagaaaa cttaagtaag agttgagtta
    12901 ataaattgaa ttcctaaatg agatattttg tagcccatat tttgtttgcc gacatcacct
    12961 aatactatgg agctaatcgt tttttctaac agaccaaaat cagataatct atttaagcta
    13021 ccatctatta gctcttgtga atagccagac atgctgatat ttccagtgaa attttcgttt
    13081 ccacgactca ttaagcatgc taacaattga atctcaatcg atgttaattg attcagtaca
    13141 agaagataat actcctcgtt atcccatgaa gcgttgttaa ttcgaagtaa acaattctta
    13201 taaagatttt tatacatatc tattttattt tgtgcctttg atttttctac ctcgtcattt
    13261 atcccttcga atattccaat gagttggtcc ttgttctcta agttgaaaac atcttctgga
    13321 atttgatcgc gaatgttttc taatgattcg tttaaatttt tgtagaattt ctcgatgcgt
    13381 ttgaaacgct tctcgttttt agacccaaaa taaagtgact gcacaacgcc accaatataa
    13441 ggaattgctc caataccgcc ttcaactatt aattcaagat attctttttt ggttagtttt
    13501 tcattgctat tatccatagt tacctcctat tttgtcattt cctttacaag cattgtgact
    13561 cctgatggca ctcttttgta taggttttat tattgtgcac aatctaactc ttagtggggc
    13621 gtggttcatt tattttttac ttactttact attaatcata tcccattgag cataggattt
    13681 ttctatgttc gatcctcttg aactaatttc tcctgtaaaa gttactttat cgccctttgt
    13741 aaaggtatca gcattgttta agtccttcgc aaaaattaca tatgaaactt taggtgttgc
    13801 agaaatatca ttccaacttt tatcatcgta ctctttgtct gctataatgg ctattctagt
    13861 agtcattgat tcgataacag ttccttcaaa agtatatgtt ttaccactaa tatatttagt
    13921 aaaagctgta ttttgctcag tagctggagt aatagaataa taactatcta agaaagcacc
    13981 ccagtcatga ttatttgtct ctaaaaattc ttcaaattga gttaggcttt tattgctgct
    14041 gttagatgta gtagtttgag tatcagatga tgagctttcc tgtttgttat tgtcatttgc
    14101 ctcgtttgat gaggaaagcg agaaacctac ggtagtaaaa ataatcccga taataaggag
    14161 aatcataggt gttttcttat ttgttttttt gataaatctt acaattaaca tgattaaacc
    14221 tataataaat aaaacaatac cagtaaaacc aaatataccg tccattaata gatcctcttt
    14281 ctcttttgat attgaatatt ataaaccaag caattctttt ttcttaaaat cgaattcttc
    14341 ttgattaata atcccttcat ctaacaactc tttatacttt ttaatttcat ctgcacttga
    14401 aatttgtgtt tctttggaat tattatctaa tatagattgt tgcttctcta tatatttttt
    14461 taaattttcc atttcattcc aatatttttt tgtaaacata acggtgtttt catctttcgt
    14521 ggccgctaat atacctccgc gactttcgtt tccgcctagc aaggtaaatt gtatataacc
    14581 attactcata cctggttttt ttaattgtac ggctgatata tttttaaagg ggatagtttt
    14641 ttctccttta ataccttgat taactaaatt tataaatcct tttctaataa ttgaaataga
    14701 atcatcagtt actttaatca aggtttttcc tggagatttt attaaaatct ctttttccat
    14761 tttttcttcc tcatttctgt gatatgattt tttttgtaga agctcatata tgaggaaaga
    14821 gcccgtgttg cagcacgggc ttttttaatg ttttgctgaa atcggttttt ttaataattc
    14881 ttgatgtatt catacatatt gccttgcgta agaatatttt ttcttaaaat agcattggca
    14941 gacagcataa caagtgtatc tgagcttatt ctgaatagaa taatactcca taaatttttc
    15001 gagattaaac tgagattcat cagtcagttc gttctcaata tagatattta ataaaattag
    15061 aatagctatt ttatctgctt ctgtttcaaa ttttgagtga aaagttgtag aagtatcgta
    15121 caaaactgaa aattcaaaat gagaggcgct gaaatgtgca agttcatgag ataaatgaaa
    15181 ggcttctgca gtttcaccgt atagattttc attcaaaaaa atgattctgg gttttggata
    15241 gtagaaacca ggctctttca tctccatata aactactttc aaattgtatt cgctcaacat
    15301 ttctttcaat ttcaaataca tacaaaccat cactccaact attcattttc ctctaaagct
    15361 ttagcaattg caatcgcttt acgcattgtc tccttagata tttcttttcc atcaaaagaa
    15421 aaaacagtat cgtcttctga taaatccaca tgtttagggg tttctctttc ttctctacct
    15481 agaaggtagt ctacagagac atcgaaatag tcagcaatcc gacttaattc atctgaatta
    15541 ggagtattat ttttccactt tgctagataa ccgttagaat atccaaaatt aatttctaac
    15601 tgccgtatag atatcttttt cctcttcgcc aagtctttta ttatttcata ggtattcatc
    15661 gataaaacaa cctttctaaa tgtttacaaa aaaagtttag aaaaatacgc agaaataatt
    15721 tgactaattc tgagtaatga gctatactat atcttgtaaa cagatttaac aactaaaaag
    15781 acaacaaaaa acactattga tatgtaaatg cagaccgcca agaaagcttt aaaatcaatg
    15841 tttttatgtc ttatttaatt atgctcttag tatagagtat tactcagata ctgtcaattg
    15901 aatttagaaa aaagttgtta aatttgttta cgaatataaa agaaaggaat gagaaatatg
    15961 aacacaccgc aaatttttaa tttcgaacaa aacgaagttc ggacaatttt agtaaatgat
    16021 gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg gttattcaaa cactaaagat
    16081 gctttgtcgc ggcatgtaga tttggaagat aagatggggt cgcgaatcac gacctcaggt
    16141 caatcaagag agatgacaat catcaacgaa tctggtttgt acagtttaat cttaaaatca
    16201 aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa gtgaagtgtt gccagcaatt
    16261 agaaaacatg gaggttatct aactccagaa aaagtagaag aagctttgct taatccagat
    16321 acaatcattc aattagcaac tcaactaaaa gaagaaagaa ctggaagatt aatcgcagaa
    16381 caaaagattg cagagtacga acctaaaatc tcctatttag atagcatatt atcttctaca
    16441 gattcagtaa caattagtca gattgcagca gattatggga tgtctccaca acagatgaat
    16501 aaattacttc ataaactagg tattcagaaa aaagtcggta accaatggtt attgtgcaaa
    16561 aaacacatga accaaggata cacaaaatct catacaactg agatcccgaa agccgatggt
    16621 ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag ggcgtctatt tatctacgaa
    16681 ttactaaaaa aagaaggata ttaccctcaa atggatttag aggaaattgg ttagaaagga
    16741 gttttagtat gactgacatt gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat
    16801 atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc tgaaagattt ggaattagca
    16861 aaagctactt atctttaatt ttaaacggta aaaagacttc tgcagaagca aacagaatta
    16921 tagattcgat tatcactatg tacgaattgt aaagggagga acagctaatg caatatctag
    16981 aagcaaaaat cccaattcca gaaggctatg taattatctc ccaagtggat tatgaggagt
    17041 taaaaaaagc tgatgatact ggtagatgga tgacgttgcc agaagtgcta gaacggatta
    17101 acagaaaata tgattggttt acttctagag ttttaaagaa cccaagatat agaaatatta
    17161 tcgatatcga gaaaaacaaa aatggatttg tctattatcc agttgaagga agagacacat
    17221 atctattttt aagaagtaaa acacttgaat ttttagaaac aaatttttcg gaaatcttaa
    17281 ggaggcaagc ggatgggaaa atttaataga gcgctagtgt tcagcgcacc gctaatcatc
    17341 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
    17401 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
    17461 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
    17521 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
    17581 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
    17641 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
    17701 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
    17761 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
    17821 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
    17881 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
    17941 tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt
    18001 taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga
    18061 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct
    18121 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
    18181 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
    18241 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
    18301 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
    18361 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
    18421 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
    18481 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
    18541 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
    18601 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
    18661 tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa
    18721 ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg
    18781 ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta
    18841 agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg
    18901 caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac
    18961 tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa
    19021 caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag
    19081 atgatttagg caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc
    19141 attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag
    19201 agtacgacag agataacttg attgatatct cttattacgg accaggtaag aaatacattg
    19261 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
    19321 tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg
    19381 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
    19441 tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
    19501 ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac
    19561 acggaaaccg aattatttcc tgttttagat tatcgaaacc aaccagttgt taaaccaaat
    19621 gcaatgcaaa tcaataactc attgaagcga tgctttgtga aagcattggc tctacacgga
    19681 ctgggattat atgtatttca aggggaagat attccaacac cacctagaat cgatacaaag
    19741 aaattaaaca tgctagagac gattctagaa gctttcaatg agcagatggg taaagatatg
    19801 accaaaacct taatcgaata tgttaatgag cagacagata aattagggct cttagctgat
    19861 aacgttgaaa ctattgaaca gttaagctat gagcaatgtg ccttgatgga gcgagcaata
    19921 gcagctaaga gaaaagaatt agataagaag tgatatgagt gtttaaacca ttaatcgatt
    19981 catattcagc ggttctgaaa aagttcaaag gaaaagacat aagcgcaacc atcaatgagg
    20041 aagtgaacat tgatcgacta aagacgatgt atgacggcta cgatggtgat cgagtcattg
    20101 aaattcgttt tattgatcct agacgtttca ccgtacagca acgaaacttc atctatgcgc
    20161 tgataggcga tatttttatc gatacaggca tgccaacgga cttctggaag gaattcttct
    20221 actttcgttt tgaaggtgtc acagggcgca aaataagcct gaaagacgaa tcgaatacaa
    20281 ctgtgagtga tgccaatgtc ttagcaaata tcatcttaga tttcatcttt gaacatcata
    20341 ttcctttcaa agaaggctat gagattttac ctgcgaatca agaatattac ttctacaaat
    20401 gcatcacaaa aagagtctgc tgcatctgtg gcaaaacagg agctgacatc gatcactttg
    20461 acaaagcgct aggaagacga aagcgcaaag aagttgatca tgcagagtac acatttgcag
    20521 cactctgcag aatccatcac acagagaagc acaaaatagg tgtgattaat ttcaaaaata
    20581 aatatcaaat caaagggatc aaattaaacc agaaaacaat caaaaagtta aatattggag
    20641 gggaaaaatg acagaacatc gaagttatta tgcgattata ccagccaacg taaggtacga
    20701 caaaagactt aaaccaaata ctaagttgtt atacggagag ataacggcct tgtgtaatga
    20761 aagaggcttt tgttgggcag gcaatgagta ctttgcagat ttatatggtg tgaataaaga
    20821 gaccatatcg cgatgggtaa gtgatttgat taagtttgga tacttgaatc gggaaatcat
    20881 ttacaaagag ggtaccaatc aaataatcaa taggtaccta cgaattaatc aataccctat
    20941 tgacgaaaaa cgcaataccc ctattgacga aaaagtcaaa gataataata catctattaa
    21001 taatacattt aataatacaa aagaatatat aagagagtta ccgccttcga aaaaatcgaa
    21061 ggctaagccc gtccgtcata aatacggaga gtataaaaat gttcttttgt cagatgagca
    21121 gatggagaaa ctcaaaacag aattccctaa tgattaccaa gagcgaatcg aacgactgtc
    21181 agagtattgt gaatcatctg gtaagactta taaaaactat ttggcaacta ttcgaagttg
    21241 ggcaaggaaa gaaaaaagtg aacctaagaa cgcaagcagt ggatacaagc gcacaggaag
    21301 acgagagaag cttcctgaat gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct
    21361 agaacgagct aatagacaat caaaagcacc attctaagag gtggaaaaat gaagatcgat
    21421 tatctagaac taattaatga aatagcgaat tataaaaagg gcgaggaatt agacgtcctg
    21481 agagacgtat atgatcaact cgaagaagct ggaattgaag gaattaagaa tgatcgttcg
    21541 agttggagta aactcagata ctatttcgca ctctatatcg atggaacgca attaagaaat
    21601 ttagcatata ccaaattact attcattgat tgtgttaaag gcttgcaaaa acatcttaat
    21661 gaacttgagc aggtgtaaac aagatggacc taaagacatt tacagcacag atcgaattaa
    21721 tgcatcaaga agctttaaga caaagtgtgt cgtacgaaga caagtggctc aacacatttc
    21781 atggcggacg tgagagcgca cttgatcaag tactcaaatt actgaaagga gaatgtcggg
    21841 atggataaga aagcggcaat gcagcgaatt atcgaattga cttattcaga agattggcag
    21901 gaagataaaa aaatagttgc agaagtccaa aagctcggta aatcaatgtg gactgaaaag
    21961 cccaaacgga gaacgccgag aaagattgca atctggcatg gtgatcgaat tctagtaacg
    22021 ggtaccgcta aacagttatc tgaaattact ggtctgagca aaaatattat ttgggataga
    22081 gctaggagct tatggattga ttcaaaagga agacagttta ggtatgtgga ggagaaataa
    22141 tggacgaact aatcgcgaaa gtagagcagt gggcaaaaga taagggattg gatcaagcgg
    22201 atttcagcaa gcaaatgtta aaaacggtcg aagaaatcgg ggaagttgcc gcttctctag
    22261 ctagaaaaga tgaacatggt ttaagagacg gaattggcga tgtagtagta accttgatca
    22321 tcttggcaat gcaaaatgat atggatttat acgagtgctt ggattttgct tatgacgaaa
    22381 tcaaaggtcg gactggaaag atggtcaatg gtgttttcgt taagtccagt gacctgaaat
    22441 aattccatta atggaagaaa taactgaata ggaggataga catgattgat gcaaaacaag
    22501 tgcaaaaaca gaaagatgga atgttgatgt ttgaagcata tgttctacca tttcttaatc
    22561 aatttgaagt tttggaatgc tctgccagcg gagaagaatt ggagtatgtt gtaattcgtg
    22621 aaaccaaaga aaacgttcag aagctaaacg aatttctttg cactattaac tgttgggata
    22681 tgatagctcc aggattttta tgtccagcta tgggagaatt tttggaatat tgtcgtcttg
    22741 aagatgcagg cactttggat ttagcttatc tggtatacaa ctacctaaac atcaacacag
    22801 atcatttgtg gtttggcact gccgaaagaa aatgggtagt ccgctaacca ccaatatcaa
    22861 tgaaaaaagg cggtttcctg aaagtggaat tgccgccatt gctgtaaggt tctttacgcg
    22921 actcaaggtc acagaaatat aaatgcaaat ctcttttgaa gtgatccaaa gctctcggaa
    22981 aatggaagtt ttttgtttca aagaataaat gagatttgcg tcaagaacct gagagttatt
    23041 tccaaaaaag cttccaaaga accgaatgag ctaaggacat catgtgtaaa cacataatat
    23101 agctctcctt tcacggctaa tcataacatg aaaaatattc gatgtcagta ggaaaaaagg
    23161 tgaattatga aaagaatatt aaattatccc ggcagtaaca gattctgcaa ttaaactccg
    23221 aagctgatgt atcagtatca actgtagaat attatgagcg tggagaaagt caagcagtca
    23281 caaatgtaaa aatcgataac gtcaacggtc ttatctgttt agatgaaggc gatagtatga
    23341 atgaaattgc tgcagaccat gaattatcag aaactgcttt aattgatcaa atcgaattag
    23401 ctagacaata ctttgttcca tatgcagata cctggtccaa gcataagaat gacatcattt
    23461 ttccagagga acaatgagag caactgatcc agtaattatc cttgaggaag ccaaatttat
    23521 ttggactcac gaagagatag agcaagcacg cttgctcttt tctcaaggag ttaagccgag
    23581 caaagtagct gaaataatgg gtcaaaagat tcttgatgtc ggattgcttt tgctccatct
    23641 agcagaaaaa atttgatttg aggtgaaaat gatgattcaa cttgcaggca tccaaacagg
    23701 gaaaatttat ttttctggag aaagcaaaag cgaagccaac caatggctat tgaaaactta
    23761 cacgaacaat aagaagctaa ggaaaaaata tcctgatttg ttgctaaagg atgatcagat
    23821 tatgccggaa ccgatgattt tgacccgtaa ggagggaagt tgaaactaaa aaaccagccg
    23881 tgtgggctgg agttgctatt tctttttaat aatgagtttt tctaaattta attcattctc
    23941 acgagtttgc gtcc
//
LOCUS       DAZKCE010000039        23745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000039 DAZKCE010000000
VERSION     DAZKCE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.039"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..844)
                     /locus_tag="ACG8KQ_001341"
     CDS             complement(110..844)
                     /locus_tag="ACG8KQ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9104283.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1171..1581
                     /locus_tag="ACG8KQ_001342"
     CDS             1171..1581
                     /locus_tag="ACG8KQ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9104284.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1574..2275
                     /locus_tag="ACG8KQ_001343"
     CDS             1574..2275
                     /locus_tag="ACG8KQ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9104285.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2275..3861
                     /locus_tag="ACG8KQ_001344"
     CDS             2275..3861
                     /locus_tag="ACG8KQ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104286.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3880..4101
                     /locus_tag="ACG8KQ_001345"
     CDS             3880..4101
                     /locus_tag="ACG8KQ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9104287.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4098..4859
                     /locus_tag="ACG8KQ_001346"
     CDS             4098..4859
                     /locus_tag="ACG8KQ_001346"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9104288.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5239..5748
                     /locus_tag="ACG8KQ_001347"
     CDS             5239..5748
                     /locus_tag="ACG8KQ_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9104289.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5823..6362)
                     /locus_tag="ACG8KQ_001348"
     CDS             complement(5823..6362)
                     /locus_tag="ACG8KQ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9104290.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="ACG8KQ_001349"
     CDS             complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="ACG8KQ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9104291.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7375..8226
                     /locus_tag="ACG8KQ_001350"
     CDS             7375..8226
                     /locus_tag="ACG8KQ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9104292.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8254..8889
                     /locus_tag="ACG8KQ_001351"
     CDS             8254..8889
                     /locus_tag="ACG8KQ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9104293.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8909..9682
                     /locus_tag="ACG8KQ_001352"
     CDS             8909..9682
                     /locus_tag="ACG8KQ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9104294.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10090..10887
                     /locus_tag="ACG8KQ_001353"
     CDS             10090..10887
                     /locus_tag="ACG8KQ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9104295.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10865..11278
                     /locus_tag="ACG8KQ_001354"
     CDS             10865..11278
                     /locus_tag="ACG8KQ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9104296.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11262..13907
                     /gene="mutS"
                     /locus_tag="ACG8KQ_001355"
     CDS             11262..13907
                     /gene="mutS"
                     /locus_tag="ACG8KQ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9104297.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13925..16033
                     /gene="mutL"
                     /locus_tag="ACG8KQ_001356"
     CDS             13925..16033
                     /gene="mutL"
                     /locus_tag="ACG8KQ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9104298.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16055..16615
                     /locus_tag="ACG8KQ_001357"
     CDS             16055..16615
                     /locus_tag="ACG8KQ_001357"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9104299.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16700..18688)
                     /gene="treP"
                     /locus_tag="ACG8KQ_001358"
     CDS             complement(16700..18688)
                     /gene="treP"
                     /locus_tag="ACG8KQ_001358"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9104300.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18911..19624
                     /gene="treR"
                     /locus_tag="ACG8KQ_001359"
     CDS             18911..19624
                     /gene="treR"
                     /locus_tag="ACG8KQ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9104301.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19701..20147
                     /gene="msrB"
                     /locus_tag="ACG8KQ_001360"
     CDS             19701..20147
                     /gene="msrB"
                     /locus_tag="ACG8KQ_001360"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9104302.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20317..20919
                     /gene="ruvA"
                     /locus_tag="ACG8KQ_001361"
     CDS             20317..20919
                     /gene="ruvA"
                     /locus_tag="ACG8KQ_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9104303.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20932..21933
                     /gene="ruvB"
                     /locus_tag="ACG8KQ_001362"
     CDS             20932..21933
                     /gene="ruvB"
                     /locus_tag="ACG8KQ_001362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9104304.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21962..22540
                     /locus_tag="ACG8KQ_001363"
     CDS             21962..22540
                     /locus_tag="ACG8KQ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9104305.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22592..23074
                     /locus_tag="ACG8KQ_001364"
     CDS             22592..23074
                     /locus_tag="ACG8KQ_001364"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9104306.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23191..23565
                     /locus_tag="ACG8KQ_001365"
     CDS             23191..23565
                     /locus_tag="ACG8KQ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104307.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7760 a         4282 c         5087 g         6616 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaataaa
       61 aaaacttggt acgcaatcgc tgtaccaagt ttttttattt agcttttgat taagccataa
      121 ataataattt atataagaat actgctacga caccagcaca gataggtgct acaactggta
      181 cccatgcata tccccattga gaagaacctt tatgtttcaa tggaagaagt gcgtgtacaa
      241 gacgtggagc aaagtcgcgg gctgggttta gtccaggacc tgtaggacca ccaagtgaag
      301 caactaatgt ccatacaagg aaaccaagtg ctaagtgagc tgtaccaaga ttatcttgga
      361 agaatggtgc ttttgtaata cctagggcac caaagaataa aacgaatgaa ccaaagaatt
      421 cattgacgaa accattgaat ttagaaccaa cgctgttgat tgttgagaat gtacctaaca
      481 catggtttac gtcttctgtt tggtcgtagt aaggtttgta acaagctact acgatcaatt
      541 gtccaatcgc tgcaccaatc aattgtgcaa tgatataagg aacaacttca gcccaaggga
      601 atagaccatt agatgctaaa gcaattgtaa atgctgggtt gatgtggtta ccgctgattc
      661 caccaaaaat cattgctggc atcataaccc cgaatccata cccgacagca atcagcatcc
      721 agtcactgcc atatcctttt gtacctttta aatctacgtt tgctacagca ccgttaccta
      781 gaacgattag taatgctgta ccaatcattt ccgccgcaag acgggtcatt agtgagtaat
      841 ccataaatac ctccaaaagt tataaaaatg caaaaaaaaa agaaacatca acacgaaaag
      901 catcggtgca gtgtttcgtg tcggaaggat ttctgcagtc ttaacaaatg tgaataaaaa
      961 aatgaattcc attacttata agaaaccaga ccaacagtaa aactccaact ctttttaaaa
     1021 gttctccaca aatcaccaag cttaactata aatataccaa aaaaaaaacg ttttttttct
     1081 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
     1141 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
     1201 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
     1261 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
     1321 aaaatcggag caactggtga ttttttattg aataatctag ccattttatt tgttcctgct
     1381 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
     1441 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
     1501 gaagctttgc tgggctatgt ccgtaagaaa aaagcagaaa gggtatctgt aaacgaaatg
     1561 gaggagaatc aaattgactg agttttttgt gaatccatta tttggcttaa gtttgacttt
     1621 aggagtgtac gtattatctg acaaattttt gcggaaatat aaactgcctg ttttgaatcc
     1681 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
     1741 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
     1801 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
     1861 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
     1921 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
     1981 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
     2041 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     2101 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctattggta cagcaaaagc
     2161 aatcgagatt ggtgaaatag aaggaatcat ttccggactt gcgatatgcg tcaacggaat
     2221 attgaccgtt ttattgctac cgttattctt tcagcctttt gctggcttgt tttgatgcga
     2281 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     2341 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     2401 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     2461 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     2521 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     2581 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     2641 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     2701 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     2761 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     2821 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     2881 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     2941 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat atatgctagg tgcctttttt
     3001 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     3061 gacaatctgt tgttccagcg attttgccag gtcatggagc cggtatttcc taattatttt
     3121 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     3181 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     3241 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     3301 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     3361 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     3421 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     3481 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatggggcg gtatctgact
     3541 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     3601 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     3661 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     3721 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgact
     3781 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     3841 caaaaactat taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta
     3901 ccagaattga aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat
     3961 gttgttctcg tatccgatga tgaatttgat ggctgtccgg tgacgccgta cttattgatc
     4021 aaaacaagtg acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat
     4081 gattgggcga tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta
     4141 tcacaggagc tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg
     4201 cttttgtatt tggattagat caaaagttcg gtaaaatgga agaaatttct ttgcgatttc
     4261 ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg
     4321 tatcgaaatg ccaaaatcat tttggtgaag ttacgatttt actgaataca gcggggatat
     4381 tggatgccta caaacccttg atggaaactg acgaatcgct gtgggatctg atttatgaga
     4441 cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct tcctgatatg atccgccaaa
     4501 aaagcggaac gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga
     4561 tcgcttatac aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt
     4621 acgcaggaca gggtatccga gtaaaaggaa ttgctccagg agccatccaa acaccaatga
     4681 atgcggctga ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag
     4741 tcaagcgatg ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc
     4801 aagcaagcta catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac
     4861 cttgtgtttt tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat
     4921 aaaaaaagtc gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt
     4981 tttaataatc gttaatattc gaaactaagc ggcttttcac gacctcttga tataaacgat
     5041 attcaaaagc ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt
     5101 tttatttgac cattcttatt acttgttgca gggcagcttt ttcactacct tctgcgacac
     5161 ttcagttgga attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt
     5221 gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg
     5281 acagatgtga ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg
     5341 tctgtcttta gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc
     5401 ttgtataaca aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct
     5461 tctcctcttg gactgataga tgttgcagta ggaagcgtca gcacgctgct tgtattgctt
     5521 atcaactata aaatcactag tcgcatcaaa aacatgaaaa tcaaaatggc cgtgacagca
     5581 tgcgtatttg ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta
     5641 ccttttttct tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt
     5701 gggataatta tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa
     5761 tttaaaatag ggcagcatgg aagagactga atgttgagtc ttcttccatg ctgctttttt
     5821 gattaatttg tatgaagaaa tcgatttagc cgttttttca tcaagatacc gatataagct
     5881 gctccagttg ccttgattgc ttccggcagt aaaaacggca gtagtccact ggcaaatgca
     5941 gttgtaaaag taagatcacc acttattttc agccagatcg ttccgcagat caatgcagca
     6001 agagagccca ttagattggc aaagattgca tataagtaat gagcatccgt tctttcgatc
     6061 atccaaccag ttatccacgt ttgtaagaga aatccaatca aatatccgcc agttggccca
     6121 aataaaacag ctatacctcc agtcatccct gaaaatacag gaagaccaat caagccaagt
     6181 aaaagataaa ctccaacaga tatcaatcct agttttcttc cgagaaaagt tgctgttaat
     6241 ccaatagcaa atgtttgccc tgtcaaagga accaaaccaa aaggaatggt catctgagaa
     6301 aggacagaaa taatcgcggc aaattccgca gcaagtatca attctttcaa tgttcggttc
     6361 ataacgccct cttttcgtta acttaatatt tttatttggt taacgaaatt gtaaaacaaa
     6421 gtgattactt tgtaaataga aaatataaaa aaagaccagc tatccaatag atagcggtct
     6481 ttttcaagaa agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc
     6541 gtcgatttct ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt
     6601 ttcagcgtca aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac
     6661 tgttacgatg ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta
     6721 tgaagggata tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg
     6781 tgcttgacga cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc
     6841 tagtaagatc atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa
     6901 caagttacgg aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt
     6961 taattgcatg ccgtattctg attgtttgcc acggctatta ggtccatgtt gacctggtgc
     7021 gtaagggcga cgtgctaatt cttttcctgt gcctgacaat gagatgccta agcgacgaga
     7081 aattttccat gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt
     7141 tttggagtaa aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca
     7201 cctttgcagc cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt
     7261 attatttttc tgctgcatta ttttacacaa attacagtat atgatagatc atccacgaat
     7321 gtcaagataa ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga
     7381 gggtatgtgc aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta
     7441 tcaccattat ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac
     7501 agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg
     7561 gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt
     7621 ttatacgcat ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgccgaatta
     7681 ggaacatcga atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt
     7741 aaggcagcca agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt
     7801 ggatttctaa tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta
     7861 gaatcaatag cagtaaaact agcaaaacct tcagcaacga ttttggtgga agggatgttt
     7921 gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgtcgga
     7981 aaagtgatca cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg
     8041 atagcgaatt ttcctgcctt tacgcttact tactttgctt ctcacggaca gatggatgga
     8101 atgacagtca gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt
     8161 ggcggtttag taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg
     8221 gactaaaaag tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat
     8281 gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta
     8341 cctaagccgg aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct
     8401 atttctgaag ttttagcttt tgttcattat tattatgaac aaatcgaaag agagtatcag
     8461 tatgtagcga tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc
     8521 gtattgttgc cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa
     8581 gtatacagca catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt
     8641 gaaaaaaagg aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa
     8701 cagcaaacag acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa
     8761 ttctttatcc aaaaagcaat tggttgggca ttacgcgatt atagtaagac cgatgccgat
     8821 tgggtaaaaa gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag
     8881 tatttataag aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga
     8941 tggaacgttg ttagattcaa acaaacaagc tttagatagc acaagagaag cattggaaaa
     9001 attacgcaaa gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca
     9061 agagatcatt cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt
     9121 tttagaccat gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt
     9181 tcaagaagcg catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg
     9241 ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt
     9301 gcctgaatta gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt
     9361 cgatcaaaca tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca
     9421 tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt
     9481 agcagaacaa gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga
     9541 ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt
     9601 acaagcagaa gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt
     9661 gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     9721 gcccagccaa acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga
     9781 tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa
     9841 ataagaaggg atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg
     9901 aagaagtaga tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata
     9961 gtcccatttt tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt
    10021 ttgacctttt ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag
    10081 gaatttagtg tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg
    10141 attactgaat atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat
    10201 ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa
    10261 gacggcgtag atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag
    10321 tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga
    10381 caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct
    10441 cgttctttta tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa
    10501 gccagaaaac gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa
    10561 caagcaatgg gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac
    10621 gttcagacaa atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg
    10681 atgacaggtc cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt
    10741 ctgactgcat taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc
    10801 tgtatcttgg aattggatga tacgacaggt catgcaaaag aaatccagct gattcagatc
    10861 aacgatgatc gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga
    10921 aattcaaaaa gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg
    10981 ctacaaagaa cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa
    11041 aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga
    11101 aaaagaagca atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt
    11161 tgtagcttat agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat
    11221 gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa
    11281 atactcctat gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt
    11341 tctatcgttt aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc
    11401 ttttggaatt aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg
    11461 gtgtacctca ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag
    11521 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
    11581 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
    11641 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
    11701 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
    11761 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
    11821 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
    11881 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
    11941 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
    12001 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
    12061 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
    12121 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
    12181 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
    12241 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
    12301 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
    12361 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
    12421 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
    12481 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
    12541 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
    12601 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
    12661 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
    12721 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
    12781 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
    12841 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
    12901 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
    12961 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
    13021 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
    13081 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
    13141 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
    13201 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
    13261 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
    13321 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
    13381 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
    13441 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
    13501 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
    13561 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
    13621 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
    13681 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
    13741 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
    13801 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
    13861 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
    13921 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
    13981 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
    14041 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
    14101 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
    14161 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
    14221 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
    14281 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
    14341 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
    14401 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
    14461 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
    14521 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
    14581 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
    14641 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
    14701 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
    14761 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
    14821 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
    14881 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
    14941 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
    15001 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
    15061 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
    15121 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
    15181 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
    15241 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
    15301 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
    15361 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
    15421 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
    15481 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
    15541 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
    15601 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
    15661 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
    15721 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
    15781 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
    15841 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
    15901 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
    15961 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
    16021 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
    16081 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
    16141 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
    16201 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
    16261 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
    16321 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
    16381 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
    16441 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    16501 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    16561 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    16621 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    16681 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    16741 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    16801 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    16861 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    16921 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    16981 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    17041 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    17101 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    17161 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    17221 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    17281 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    17341 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    17401 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    17461 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    17521 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    17581 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    17641 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    17701 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    17761 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    17821 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    17881 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    17941 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    18001 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    18061 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    18121 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    18181 gaaaatggaa aattgcttca ccgggtaacc acaaaaaggc attgacacca ttccaaaact
    18241 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    18301 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    18361 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    18421 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    18481 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    18541 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    18601 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    18661 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    18721 gcaagttatt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    18781 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    18841 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    18901 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    18961 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    19021 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    19081 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    19141 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    19201 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    19261 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    19321 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    19381 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    19441 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    19501 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    19561 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    19621 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    19681 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    19741 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    19801 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    19861 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    19921 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    19981 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    20041 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    20101 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    20161 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    20221 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    20281 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    20341 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    20401 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    20461 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    20521 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    20581 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    20641 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    20701 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    20761 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    20821 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    20881 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    20941 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    21001 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    21061 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    21121 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    21181 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    21241 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    21301 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    21361 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    21421 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    21481 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    21541 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    21601 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    21661 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    21721 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    21781 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    21841 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    21901 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    21961 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    22021 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    22081 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    22141 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    22201 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    22261 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    22321 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    22381 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    22441 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    22501 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    22561 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    22621 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    22681 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    22741 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    22801 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    22861 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    22921 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    22981 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    23041 gattgatgaa aataaaataa caaacaattt gtaaagttaa cgctttcttt ttaaggcgat
    23101 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    23161 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    23221 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    23281 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    23341 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    23401 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    23461 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    23521 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    23581 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    23641 gctgatatca gtaaaacgga acgttttagg atgagtatca cagcatgaca gcattgggtt
    23701 gattagttga atcgattgga tggtacaagt ttttttgata aaaaa
//
LOCUS       DAZKCE010000040        23447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000040 DAZKCE010000000
VERSION     DAZKCE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.040"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            314..478
                     /locus_tag="ACG8KQ_001366"
     CDS             314..478
                     /locus_tag="ACG8KQ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104308.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            475..705
                     /locus_tag="ACG8KQ_001367"
     CDS             475..705
                     /locus_tag="ACG8KQ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104309.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            890..1435
                     /locus_tag="ACG8KQ_001368"
     CDS             890..1435
                     /locus_tag="ACG8KQ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9104310.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1532..1753
                     /locus_tag="ACG8KQ_001369"
     CDS             1532..1753
                     /locus_tag="ACG8KQ_001369"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9104311.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1910..2074)
                     /locus_tag="ACG8KQ_001370"
     CDS             complement(1910..2074)
                     /locus_tag="ACG8KQ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFP9104312.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2176..2697
                     /locus_tag="ACG8KQ_001371"
     CDS             2176..2697
                     /locus_tag="ACG8KQ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9104313.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2684..3124
                     /locus_tag="ACG8KQ_001372"
                     /pseudo
     CDS             2684..3124
                     /locus_tag="ACG8KQ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3164..3322)
                     /locus_tag="ACG8KQ_001373"
     CDS             complement(3164..3322)
                     /locus_tag="ACG8KQ_001373"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFP9104314.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3415..4176
                     /locus_tag="ACG8KQ_001374"
     CDS             3415..4176
                     /locus_tag="ACG8KQ_001374"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9104315.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4363..5715
                     /locus_tag="ACG8KQ_001375"
                     /pseudo
     CDS             4363..5715
                     /locus_tag="ACG8KQ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5731..6951
                     /locus_tag="ACG8KQ_001376"
     CDS             5731..6951
                     /locus_tag="ACG8KQ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9104316.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6986..7663
                     /locus_tag="ACG8KQ_001377"
     CDS             6986..7663
                     /locus_tag="ACG8KQ_001377"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9104317.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7660..8853
                     /locus_tag="ACG8KQ_001378"
     CDS             7660..8853
                     /locus_tag="ACG8KQ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9104318.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8868..9194
                     /locus_tag="ACG8KQ_001379"
     CDS             8868..9194
                     /locus_tag="ACG8KQ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9104319.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9198..9533
                     /locus_tag="ACG8KQ_001380"
     CDS             9198..9533
                     /locus_tag="ACG8KQ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9104320.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9530..9925
                     /locus_tag="ACG8KQ_001381"
     CDS             9530..9925
                     /locus_tag="ACG8KQ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9104321.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9922..10293
                     /locus_tag="ACG8KQ_001382"
     CDS             9922..10293
                     /locus_tag="ACG8KQ_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104322.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10309..10869
                     /locus_tag="ACG8KQ_001383"
     CDS             10309..10869
                     /locus_tag="ACG8KQ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9104323.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10994..11305
                     /locus_tag="ACG8KQ_001384"
     CDS             10994..11305
                     /locus_tag="ACG8KQ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104324.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11329..11520
                     /locus_tag="ACG8KQ_001385"
     CDS             11329..11520
                     /locus_tag="ACG8KQ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104325.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11520..15368
                     /locus_tag="ACG8KQ_001386"
     CDS             11520..15368
                     /locus_tag="ACG8KQ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9104326.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15369..16241
                     /locus_tag="ACG8KQ_001387"
     CDS             15369..16241
                     /locus_tag="ACG8KQ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFP9104327.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16238..17287
                     /locus_tag="ACG8KQ_001388"
     CDS             16238..17287
                     /locus_tag="ACG8KQ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104328.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17291..18115
                     /locus_tag="ACG8KQ_001389"
     CDS             17291..18115
                     /locus_tag="ACG8KQ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104329.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18133..20193
                     /locus_tag="ACG8KQ_001390"
     CDS             18133..20193
                     /locus_tag="ACG8KQ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9104330.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20210..20359
                     /locus_tag="ACG8KQ_001391"
     CDS             20210..20359
                     /locus_tag="ACG8KQ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104331.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20356..20802
                     /locus_tag="ACG8KQ_001392"
     CDS             20356..20802
                     /locus_tag="ACG8KQ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104332.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20804..20941
                     /locus_tag="ACG8KQ_001393"
     CDS             20804..20941
                     /locus_tag="ACG8KQ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9104333.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            20976..21218
                     /locus_tag="ACG8KQ_001394"
     CDS             20976..21218
                     /locus_tag="ACG8KQ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFP9104334.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21234..21431
                     /locus_tag="ACG8KQ_001395"
     CDS             21234..21431
                     /locus_tag="ACG8KQ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9104335.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21442..22458
                     /locus_tag="ACG8KQ_001396"
     CDS             21442..22458
                     /locus_tag="ACG8KQ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9104336.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22573..>23447
                     /locus_tag="ACG8KQ_001397"
     CDS             22573..>23447
                     /locus_tag="ACG8KQ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104337.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQV"
BASE COUNT         8157 a         3959 c         5179 g         6152 t
ORIGIN      
        1 gcgctttgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa aagatagtag
       61 taactaagaa agaaattttt atattgtcac tgtggcggaa agggtagacg ctaagcatgt
      121 gtgctaggtc aatgcttcgg caactatgca atgttcgatt cattgccagt gactttggca
      181 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
      241 cgtgtaggtt gctattacat attagatcac tcgttgagtg gtctttttat tttacataaa
      301 ggaggtaaca acgatgtata gaccacaata cttagaacag aagtatgaag taatcactgt
      361 gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata
      421 taaacgaaag gaaaacaatg aagttatttc ttttcgcaga aggagaaaag ccaaatgaga
      481 aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg
      541 cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat gctaagagaa
      601 gctacaccaa acgaagtgga taaatgcaag ctgatatatt gcggtcatgg ctattggaaa
      661 gacgagtata tccaacaaaa cattgagagg tggatagata gatgagttac ctcgaacatt
      721 tgaaacgttg ctacatgcat tctaagaata aacttcctga cagctacact gtagatgatg
      781 ttgctataca tgtattgaag actgagagcc atagcagtcc tgatggtagc agtaaggagc
      841 agacgctcgc gtggttcaag ttctttaagt ggattaagga ggaagagtaa tgcacatgca
      901 cataggagaa aagatatata aattgagacg gcttaataac gaaacaatga acgacttagt
      961 taaacaaact ggagtgtcaa aatcatggtt atctgatatt gagtctggaa aaaagcaacg
     1021 cgttgatttt aataagatat gtgcaatagc caaacattat aaagttagct tagaatactt
     1081 tcgggatgat tatttggata gtgatgaaca cgaggatatt attcctttga caggcgaaac
     1141 aagagttaaa tcagtgaaat cgcttgaaca aagcctaaag tatctgaata acaaacaaga
     1201 acctaacctt ctagagatca aactaaaaga tactgactca gtaccagagg tttggtacaa
     1261 gggtgagagg ttggatgaat tgcctaaagg attagtagat gtctcgtatc attggaagac
     1321 tgatgatttt actaatgatg atagaggagc gaacgacatc acgattcaat acttttctag
     1381 ctttaatgat aagtatccag ataagaaaac aatcggacac aagagagata tgtaaatgaa
     1441 agaagttaga cctagagacg aaatagacaa actatacaag accaaacgat ggcgagacct
     1501 aaggcaagta gtaatagcta gggacttcgg catgtgccaa gagtgcaagc gtcgagggcg
     1561 gaacacaagg ggcacgatca tccatcacat agtcgaggcg agggaagacc tgtcactgtt
     1621 ctggtccgta gataaccttg aatgtatctg tgtagcttgt cacaacagag agcatccaga
     1681 gagatcaggc gggaagaaga aaccaaaacc taaatcacat atcgttaaaa tgtattcaac
     1741 tcctgaaaga taagtttgca gcgaaatgaa ggtagccccc ctactctaaa agattaaaga
     1801 gtaaggcttg agaagaacgg tgctgtcctt ccttcgtaaa aagaccgctt ttcaagtttt
     1861 ttggagaaaa aggaaaaagc cgaccaattt aagccggctt tggacgaagc tatttcttag
     1921 tccatttgtt tcctttttga gaagtagggg gtaatcggtc gcctggatca atttttactt
     1981 cttgtccgcc ttggacattt ccaccacgag gacccacttc tttgtaggtt ccttttggtt
     2041 tgttgtcttc gccgggttta tagagttctc ccataggaat ccctccttaa caaatttcgg
     2101 cacagcactg ccgataacct aattataagg attgtgataa cgatttcaat ctatcttttg
     2161 aaagaaggtg atattatgcc gcaaccagcg aagagtgcaa aattacaatt attaaacgga
     2221 aacccaaata agaagaatac cgaagaactc cgcaagcgag cggccgcaga agacaaatta
     2281 aaaatggcta ctgacaaaat caaaccgccg ttatggctag attcgctagg aaaggatacc
     2341 tttgagttta tcgccgatga attgctgtct gtggatttaa tcagtaatcc ggacgtccat
     2401 acaatggctc tatactccaa ttggtattcg caatacgttt ctttagaaaa acagcttcga
     2461 aaactacaac gagagtacaa gttgaactat gcgcttgcga aaaaggaggc agaggcgaga
     2521 ggtgagccgt ttaatgaacc taatgaatta attggtaatc cgctctctcg gcagatggat
     2581 acagcgtctc ggaatctccg ttcttttggc gctgatttag gactatcacc agcagccaga
     2641 gctaagttag ctattaagat ggctgatgat ggtggtgatg acgatgacga cttctaatat
     2701 tttggatatg tcttacacag aacgtgtgga ctattggcaa agctatcttg aggagcaagc
     2761 ttcttggggt ggctttttaa aatgtccata tccggaattg ttaactactt ggtatgcgga
     2821 acgattaatc gatggaagca taccagccag caaagaaaat attcaagctg ctaaacggca
     2881 tatgcgtgat ttgcagcgcc aaggaacaga tgattttcct tggatctttg acgaagaaaa
     2941 aggtcaccgg cctattagat atatcgaaaa aaaatgtaaa ccaactgaag gcgactttgg
     3001 ttcgtttgtt ttgcaacctt ggcagcattt cataattgga tccatgtacg gatgggtaca
     3061 tcgtgataca ggagagcgtc gcttccgcga ggctcttatt tttgttggac gtaaaaacgg
     3121 taagttttgc cgtttaaaaa tcgggcaaaa tcggtgaaaa cctttatttt tggctttctt
     3181 ttagtaataa ttcgatagct ttatcaagca atttagacat aggaaccata gtttcttccg
     3241 acattttttt taatccttcg tataaatctt tatcaattgc gttagaaatt ctaattctgt
     3301 tttttagtcc gtatttgtcc atgtgtgaca ctccttttta ttttactata acatccgatt
     3361 ccaccgcttg caactaccat caattgatgg tagtatataa aagaggtgag tagattgaaa
     3421 aaatacatta tttacaaaca cttaaataaa ataaatggca aaatttatat cggtgtcaca
     3481 aatgatattg gcagaaggtg gaggagcggt gggattgaat ataaaccacc aaagaacgaa
     3541 acccaacacc acagaagttt ttggaatgca atacaaaaat acgggtggga aaattttgat
     3601 catttgatta ttgaagaaga cttaacgatg aaagaagctt ttgagaaaga aaagttttat
     3661 attgaacttt atgactcaac taataagaaa aaaggttata acatcgcaaa aggcggaaat
     3721 ggcggaataa tatataaagt tcaccccaaa ggaatgctag ggaaaaaaca atctaaagaa
     3781 ttttcttcaa atcacagtaa atgggcaaag aaccataaga ataattgtat gacaaacggt
     3841 gatgttgtat ggggtgttac tcacgaacac ccaaaaggca tgttgggaaa gcaccacagc
     3901 aaagaatcaa ttatgaaaaa gaaagcatat agtggtgaaa atgctgtaac ctcaaaacca
     3961 attgtcgcaa ttgaacggga tggaactaaa agagaatttc acagcgctaa gctttgcatg
     4021 gcttactatt cgattagtac atgtgttttt taccgacttt taaaagatgg cgccccttat
     4081 gtgataaatc ctaaagcgaa ctacagaaat aaagaaaaaa tattagctat cgaaggaatt
     4141 atgttcaaac ataaagaaga taccgaggta agttagtaga ttgcgaaagg ttactaacta
     4201 ccgtaacgcg taggagttga ataaatataa tgctcccaag agtgtccgac agcagataat
     4261 ttgctgaaaa tgtacgctaa actgggccag aactgactga ccgatgaaaa tgagggtgac
     4321 ctccagagtg cgagataaaa agctcgcaga taataacaat cgaaaacatc actaatttca
     4381 ggcctttcca catacatggt cgcttatgat gatgaacaag gcgccaacgt ttacgtattg
     4441 gcaaatgctc gtgatcaagc aagcttgttg tttgataagg ccgcagaaat ggtcaaacaa
     4501 tcgccggcgc tctttaagaa atttggtaag cctaaacgat caagtattaa ttatgctccc
     4561 gccttttcta aaatggaacc acgcgcctca gatagccgga aattggatgg gctaaacact
     4621 cactttggta tttttgacga gatccacgag tttacgaatt acaatctgat caacgttatc
     4681 aagaaatcaa gaggaaccag aaaacagcct ctgatagttt atatcacaac tgctggatat
     4741 gtattagatg gtccgttgat gtcttatttt gagcaaggtg tggactgttt ggaacatttg
     4801 gaagatgaca tcgatgaacg gactttctat tatctggcaa aacttgacag tgcggaagag
     4861 gctgatgacc caagattatg gatcaaagcc aatccgaata tttgtctaat gaattttgtt
     4921 ggcatgctag atgactatgt taaggataaa aaagatccaa aagaatatgc tgactggatt
     4981 accaagcaat ttaacttgtt ttccgatatc gatgagctgt catttgtcga tatgcctacc
     5041 attaaacgaa acaataaaac catcgatatt gaaacgctca aaggtaagaa gtgtgtcggt
     5101 ggttttgact tgtccgaaac ggaagacttt accgcagccg ttttagaatt tccgcttgaa
     5161 acaggcgagg tattcatttt gcaacacaca tggatcccac aagctagatt tgatcgagat
     5221 aacaatcaag agcgtatcaa agcgtgggag aaggtgggag atctaacgat tattcctggt
     5281 gattacgtca attatgaata cgtcttaaat tggtttgtag aaaattcgaa aatctatgac
     5341 attgtaaaaa tcaattatga caaggccaag gcgctacgat taaataaaga actagaaaat
     5401 gcaggatttg aaaccaacga gattcggcaa gggtttctat cattaggtgg gccaatgcaa
     5461 aacttcaagg aaatgctatt ggacggtaag gtgattttca acaattccaa gctttaccga
     5521 tggtatctat ccaacgtcaa gctggtgatg gatcgcaact caaactggat gccgtctaag
     5581 cagtccaaga gtagaaaaat agatggtttt gcagcaagtt tgaacagcca cgccgaagtg
     5641 ttgaatatgt tggttaatcc tgtcggaacc gggaaagtaa cctattactc gatttccgat
     5701 ttaatgaata tgtaagaaag gtgtggagga atgagtattt tagatcgttt gcgttctttt
     5761 ggccgagcga agccgaaagc gagcaaacaa gagtattttt tgaatgaccc gggattgata
     5821 ccgtatttag tcggaaaaga tgaaatatca gaagggattt tttccgtaat tagccgtgta
     5881 tcgaacgttt ttgcgtctct ccctctcaaa atgatagatg tggagtttgg ccaaccggac
     5941 gactgtcctg catacaactt gttgagcgaa ggccctcgat attttacaaa gtttgatttt
     6001 ttccgggacg tggaagtttt gagaaactac caagggaatg cgtatgtgca gattttccga
     6061 aatatcaatg gagaagtagc agatatggcg ttagtaaaac ctggtgcttg ccatccagtg
     6121 attgatatgg atagcgggga gctttactac caagtaactg cgactgacaa aggcagttac
     6181 aagcaagtta tctatgtaca ttacatggaa atgcttcact ttaaacaacc gaggtttggc
     6241 ggcttggaag gtacagaccc cacaaaagta ttaacgaata ccctcggata tgatcgagaa
     6301 gtccgaaaaa tctctttaag tcagcttaaa ggaagtaatg aagggctaaa agttaagttt
     6361 gctagcaata tggatgaaga agctaaaaaa gctacagtta aaaacattgc tgatttttat
     6421 cgacaaaacg gtggactact tgtggaagaa aacggtgtag aaatcgaacg tttacaacga
     6481 gagctggtag acagcaagct tttagatact gataaagtat ctcgctccag aatcgctatg
     6541 gtctacaatg tgccggagca tttcatcggg aataaccagt cgagttactc ctctcaggaa
     6601 cagctcaata tggaattttt gacgtacaat ctagtaccga ccgttaatca atatgaagcg
     6661 gaactaaata agaaaacact atcgagaacc gaaaaagcaa aaggttatcg atataagttt
     6721 aatgtcgcaa gtttgctaag agctgacact caggcaaggg gacaattcta tcaaatcatg
     6781 cgacgaggcg gagcatactc agcgaatgat gtccgaagat ttgaggactt gcaaccgata
     6841 aataaaaccg gtatggatga ttaccatatt tccggagacc tatatccaat cgatatggat
     6901 ccaacattaa gaaaaacaac ctcgtctaaa agcgtagccg aaaacggtta ggttttttta
     6961 gtttgcaccg aagggaggtg gaaaaatgaa aaaagtgacg ttaagcggcg atgtcgtgga
     7021 taacgatacc gcgtggcttt atgactggtt tgggatcaat tgtatctcac cagggaaaat
     7081 ttctgccgct cttacagaag cagcggggga tgaagtagaa cttgatatct catcgaacgg
     7141 tggggatgtc ctagcggcaa gcgaaatata taccgctatc cgtgcctatc cgggaaaggt
     7201 atccggaaat gttgtgagta ttgcagcaag cgctgcaagt gtaatcgctt gcgcttgcga
     7261 accgcttaga atctcaccta cggcacacat catgattcat aacgcatggg tgaccactag
     7321 tggcaacgct gaggaattaa aagccaatgc agaaatgtta agcagtgtgg atgagtctat
     7381 tgttaatgct tacgagatca aaacaggact cgatcggaaa aaacttgctg atttaatggc
     7441 gaaagatact tggttaaatg ctcaaacagc agtagcagaa gggtttgcag atgaaattat
     7501 gtttgcagaa gcaccagtaa cggtactcaa tgcctctcaa ccggttattc caaaaaacgc
     7561 agtaactaag ttgaaaaatt taatactcaa agcggaagca ccgcaagaag aaacactctt
     7621 acagaaaaaa ctaaaagcct taaatggagg gaaaaacgaa tgaatttaga acaattaaaa
     7681 aatgcgtggg tcgaggcggg aagtaaagtc tctgacttaa atgcacaact caatgcagca
     7741 ttggttgacg atgaaaaaac agaagaagat gtagtaagtt tgcaagcaca agtaaaagca
     7801 gcacgggcta aacgggacgg attgaaagag caagtggcaa atatggaagc cgaacaagtc
     7861 ttaaacgtca aaaaagaacc attagataaa aaagatgaaa acttgaaaaa caagtttatc
     7921 aaagacttta aagcgatggt caatggtgat cctgctatta tggctacttt gacatctaat
     7981 acggatgaat ctggtaatgc tatcggattg actattcctg tagatgtgca aacgactatt
     8041 catactttgg ttcgtcggtt tgactctttg caagaatacg taaacgttga aaaagtgacc
     8101 actaccagcg gttctcgcgt ttatgaaaaa tggtctgata ttacaccgtt gactgctttg
     8161 gatactgaag acggtgaaat cccagcaaat gatgatcctg cactttactt gatcaaatac
     8221 ttgatcaaac gctatgcagg tatttccaca gtaactaaca gcttgctaaa agatactgcc
     8281 gaaaacattt tggcatggtt gtctaaatgg atcgcgaaaa aagtagttgt tactcgcaat
     8341 acaaaaatct tggcagctat tgatggaatc aaagcggcac aaaagaaaga tgttacagat
     8401 gttgatggaa ttaaagatat cgtaaacgtc caacttgatc cagctatcga agctacatct
     8461 atgtttatta caaaccaaga tggcttcaat gttttagata aagtgaaacg tgctgatgga
     8521 tcttacttgt tacaaaaaga cgtagcttct gcaactggat ataccttctt aggtaaacca
     8581 atcaagaaaa ttgcttctcg tttcttgcca aacaaaggta cgcaagctac tcctaaatat
     8641 ccactgtaca ttggtgatct gaaagaagcc gttacattgt atgatcgcga aaacatgagc
     8701 ttgctgacaa cgaatattgg tggtggagct tttgaaacag acaccactaa agtacgcgtc
     8761 atcgatcgct tcgatgtgca actagttgat gatgaagcgg ttgttttggc tacttttaca
     8821 actattgcga acgagacacc ggcgaaagtt taaggagctg attccttatg attcttgatc
     8881 ctaaaatgga tttaggcgaa atcaaaaacg cactaaagat tgataccgat gatgacgatg
     8941 tggaagtaag ccgtgcggca caagctgcaa ttgcatacat tagaggggct atcgggaatg
     9001 ataagccctc tttttataag caagaaaatg aaacggttga tctgattaat ttagctatcc
     9061 tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg aacttgcgag
     9121 agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagttat ttgctttttg
     9181 tggaggagga gtagcgtatg ccccttatcc aaacaggaaa tttaaatcaa cgcatcaagt
     9241 ttgtccgaga tacgacggtt aaggatgagg acgggcaagt tgtcccgact tctacaacca
     9301 ttcttacttg ctgggcaagt gtgcagacac aacgcctgaa cgatattaag acgtcgattg
     9361 gtacggcttt ggaaggaaca ctgacgttca ttatccgcta ccaacaaaaa tcagagctga
     9421 ccaatgatat gaaagtgcgt tggaatggaa aaacgtttga aatcattacg attacgaaag
     9481 gcgagtttgc gaaggacttc acgacaatca ttgcaaaaga ggtttcaaaa tgagtgtaga
     9541 agtcgatgca accgaagtgt acaaagcgct tagggaagta aaagcaaacg ttcaacgagt
     9601 ggaaagccca gcacttagaa aagctgggga gtacgctcaa gaagagttac gacaaaacac
     9661 accttactgg gatggaacga agtcaaacgg taaacgtggt tcgtatatgc aagaacatgc
     9721 taagaaccat gtggttacaa gctcgataaa aaacggattg gtagaagtcg gctatgacaa
     9781 agatgtttct tggcggatgc actttatcga gttcggaaca atcaaacaac gtccaaaagg
     9841 tttcgtacaa aaaacacaaa agcaaatcga aaaacaagta acacaaatca ttgctgacga
     9901 agtaaaaagg aggctaggac tttgaaaacg gcagtatcac aagtctattc aattctgaat
     9961 agcaatgaaa aaacaaagaa cattgatttt tacaccaata gtgttccgga atcagctcaa
    10021 acagtaccta gccttccagt tggcagaatt acagagatat ccggcaacta tgaagatttt
    10081 gcaagcaaca atcctttgac cattcaattt aacgtacagg tagatgtatg ggtgtcaacc
    10141 atgaaagagg ttgatgcctt ttattttgcc cttgatgagg ttatgagggg gaatggttgg
    10201 caatgcgcat acacggaaca aacagatgac gaggacttgg aaggtgcaaa gcggattatc
    10261 aaacgatatg tagcaaatat ttcactaaat taaaaggaga gaaaatagat ggcaacagta
    10321 ggattcgaga gcgtcatttt tggcgtaaaa acaggtgcag gcggcactct aaaagaatta
    10381 gtagcagata agtcgaaagg cggagcgatc gaagctaaaa ttactggatt aggcgcaact
    10441 tctaacacaa catacgcttc aaacgtaccg ttcttcattg caagtaaagg ggtttcgtcg
    10501 ccaaaagtta cgcttgacgt ggcagactta atggataacg gcatttacag cgaaatcatt
    10561 ggtgctaaaa ccgtggatgg tgcaaatgta attggttcag aaactgaagc gccttacgtg
    10621 tcggtagtca tggttacagc gaacaaagaa ggaaaacgct tgttcatggg attgacaaaa
    10681 ggaaaattca gtcatccaga tatcgacatg aaaacagctg aagacaaagg ggtagaattg
    10741 caaaccgatt ccatcgaagg ggaattcatt tctgatgaac gtggctatgt atacttaaca
    10801 gccgtagaat cagaaggaat gaccttacaa aaattcaagg acttggtaaa taacaaagag
    10861 ggggagtagt taaccctgca tctacaccaa cgacagatac agggacacca aaagaaccag
    10921 aaccaaaaat tgatacacaa ggttagccat ttttggctag ccttattttt tgtaaaaaca
    10981 aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa aaacattcaa
    11041 acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg agaaagcgga
    11101 aaaagcagac ctaactgatt tagaagcgat tgattcaatg attgcaatta cagcagatat
    11161 tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg cgagcgagtt
    11221 agtaccggca ttagaaagtg tttttgaaca aataaatgaa ctgggaaaca atgaaaaaaa
    11281 gcagacggcg agcaaaaaga aataagtttt tctgaagcta ggaaagcaat ggatcaaatc
    11341 tacaaagatt taatcgaagc aggttggacg atgagagatg tggacgaagc cgactatcat
    11401 tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga tggtgctgat
    11461 ttcatcaaac aatttttatc ggctgaagac ttagtaaaac ttgaggaagg aggtaaataa
    11521 tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta gatagcacta
    11581 gttttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag tcgaatatgc
    11641 gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt agtcaaaaga
    11701 cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa ttaacaaagc
    11761 gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct aaccttaaca
    11821 cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg aacgacacaa
    11881 aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaatgaa atcaaagaaa
    11941 atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat gaatctggtg
    12001 cttttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag aaagctatcg
    12061 aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca gcaaatgaaa
    12121 ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata tctagcaggc
    12181 aattagaagc actcaaaagc tccagcgatc aatcaggaaa agaaatagaa gattttggcg
    12241 acaagtccac aaggtcagct aggaaactgg acggactaaa agacaaatta agctcgctaa
    12301 aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt agcagtgtag
    12361 taagtggcgt gcaaggtttg gttggcgaag cagtaaacgc atcggattca ttgatgaagt
    12421 tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc tcgaaaaaag
    12481 aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg aacacaaccg
    12541 cacaattggc atctaatggg attcctaact atacagaact aaccaaggcg gcaggtaact
    12601 tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg atgctaacgc
    12661 agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca gatgcgatac
    12721 cgggtgcttc aggattgtta caagacgcta tgttgaaaaa cggagcttat acaggaaact
    12781 tccgtgatgc aatggcgcaa ggtcaaatca cttccgacga gttcaaccaa gcaattgtac
    12841 agttaggtat gaatgacgga gcagttaagg cagccacttc tacagacaca ttgagcggtt
    12901 cttgggagca gatgaaatcc actgtaataa atgggctaca aagtattata gaaaaaatag
    12961 gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa aattctattc
    13021 ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc gttgaaaata
    13081 gagagccact aacatggatt gtcggaatca taggcggaat tacattagca gtaaaagcat
    13141 tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc ccttttgtgg
    13201 cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat acaaaatctg
    13261 aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta gttatgagcg
    13321 ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag tggctttggg
    13381 aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg aagaacagcg
    13441 ttgtgaatac ggttcagaac ctatggaatg gcgtgaaaaa cttcttcagc aatggcgttg
    13501 gagatacgtg gaataaggta gtcggttggg taaaaaacat tttcaacaaa gcaactgaat
    13561 tgaagaacaa cgtttctgat gtaatcggta acctgtggaa tggtatcaaa gacacattcc
    13621 gtagaggtat cgatacggta ttcaattggt tttcagaact accaacgaag atgaagaatg
    13681 ccattattgg cggtaaaaac gccattgttg atgcgttcaa aagtattttc aacgcagcac
    13741 ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg gtactagaaa
    13801 aattgggcgc agaacctcta caagaatggg atgtaccaca atacgctaca ggtacaccag
    13861 caggtgggca cccaatcaat ggtccaatga tggttaatga tggacgtgga gcagaaacag
    13921 ttatcacacc agatggtaga gccttcattc ctaaaggacg caatgttgtt ctaaacgcac
    13981 caaaaggaac gcatgtcttg acagcagaag agaccgcgca acttcaaggt tctaaagctc
    14041 ctaagtatcg ttacaaaaaa ggtactaact tctttggtaa catgtgggat agtgtgaaaa
    14101 aggttgctgg taatgtaggt cacacaatta aaaacgtagt aggtgacgtg tgggacttta
    14161 tttcagaccc gggagcgtta gctagaaaag tacttggggg gttagatgta ttaggtggat
    14221 tgacaaagta tccattagaa gtaggtaaag gcatcctatc taaagcaaca agtgcactga
    14281 ctgaaaaaat cactgggttg ttctcatctg gtaacttaga tacctccgta ggaacaaatg
    14341 gcgtctataa atatttggct gatgttgcta agtctgtgat gaagaaattc ccaggatttg
    14401 tggcaactag tgggtataga ccaggtgacc cttattcaca tggtaaacgt aatgccattg
    14461 atattgcatt accaggcgtc acaggaggct cacctagata cacagaagca gcaaactacg
    14521 cttttgacaa attcgcttcc aaaattggtt acgtaatcac taatgggaaa gttcgtgacc
    14581 gttcaggaca atcaggtcaa ccagcaactg gtgcatggga gccatggccc gatggagatc
    14641 actatgatca tgtgcattta aacggtgtga aagacccaca aaacactcaa atttcaggag
    14701 atagcgtggg aggcagtggg gtagaaagat ggcgcaatgt agcaattaga gcgttgaaaa
    14761 tgaccggtca atacagtact gcaaacttaa atgcattact aaatcaaatg cgtacagagt
    14821 caaatggtaa tcctaatgca gttaacaatt gggatattaa cgccaaaaat ggaacaccat
    14881 caaaagggtt gctccaagtg attgacccaa cattcagaca gtatgcaatg ccaggattca
    14941 acagcaatat ttttgaccca ctatctaaca tcttagcttc aatcagatac gcactatcaa
    15001 gatatggctc actaacaaat gcctatcgtg gagttggtta cgcaaacggt ggaattgtaa
    15061 accaacatca aattgcggaa atcgcagaag gaaacaagcc agaaattatt attccgttag
    15121 ataaggctaa acgatcaaga gcgatgcaat tattgtctat cgcaatggac aaactaggag
    15181 tttctcctaa aacttatgga aatgcgacaa ctgtttccag tgattacgaa atcctagaag
    15241 ccattgaaaa acaggcggca acaacgaatc agctgttatc gttattgctg gcatttttca
    15301 aaggaaatag ccgaactgat agagatttag ctttagatat tcagaagatc ttggttagga
    15361 ggatgtagat gcgaactgta ttactgagaa atagaataaa cgaagaaatt gatttgtcca
    15421 cagaagacta ttttgctact gggttgagta atatggggtt tgaagtaaaa aaagaacatg
    15481 tgggacagtg ggggaatttc agagaaagca gtgagagcgt tgaaatatct gaatttcagt
    15541 catctgtaat catttctgtg catgggtttc gagaaaaaga actgtacaat tcacttgtgc
    15601 agttcctatc ggaaggtccg tttgaactgg aatttgcttt tgacggcgaa acaatggtaa
    15661 gaagatgcag tctaaaatct ttaagtaaga ctgaaatcga tccgaaaaca tcgttactaa
    15721 cagacacttt agaactatat ttcacatcaa actggtattc cgtaaaacgc gaaaaattaa
    15781 ttcagcgacc gaatgtagtg aaaacacgtg gtaaggtttt tccgtataaa agatcttata
    15841 tctacactca aaacttgtgg gagaaaaaag gtgtattcaa attcaataat aattctgtat
    15901 acctaacgaa tagcaaggaa cggatgtccc cgctaaaaat tcgtgtgatt gggaagtgtt
    15961 caaatccgta ttgggaagta atccaaaact cacaaatcat cgcaacggat ggatacttca
    16021 tagatatgac tgaaacacag actctagaag tatcaagcct ttttgaagat acaacggcga
    16081 ttttaaaaga tattgcaggg gtagaatctt ctgtctacca acaacaggat tatacaaaaa
    16141 ctaattttgt tcaggcccca actggagaat tcagcattgt gtttcatgtt gggggggcag
    16201 acgtagagat tgagttatac gaggagcgtg atctgttttg attttagcag tcacgctttt
    16261 tcatcgtgat ttaatgcttt ataacgaaca tttattctct aacagttttg aatttggtgt
    16321 ggacgagatc aacgaagagg ctagtagctt tacgatggat aagtacgttc ctgtaaaaac
    16381 aggcgatttt ctactagcaa aatatattcc tagtggaaaa tttgcatatt ttggtgtaat
    16441 tacgtcgcag gaagacgaaa aaatcagttg taaaagctta cttagtttag ctgatagtga
    16501 gataccgact gcacgtgtgt caggagataa ctacgaagag catatccggc ggttgattga
    16561 atactatttg ctgaatgatc caacgaaaca actaaaagat attttagacg tcaaagcaga
    16621 aagtgcgacc tctcattcgt atcaagctac cgatacgaat aaacacaagt taagtgcgta
    16681 tattctcaat ggtttcaaaa aatacaacgt aaaatggtat ttcaagggaa ttcaaaacag
    16741 aaaaatttat acaggcatac gtgctgtaaa tgaatcaatt tacattaaag acaattcttc
    16801 tgaatttagc gattgggatg tgtttgttca ggcgccgggc gctggaaacg agaacaagct
    16861 attaatcgtt gataaagcaa tgaaagatat agagaaaccg ataatactgt caacatggta
    16921 tttggacgaa gaaaacaatt tgacacaaga tggatcgaaa gaaaatatca cgaaaccaac
    16981 tgtcaatttg gttaatatct acgatcaaac cgcagaagat aaagcatcct acgaagatgt
    17041 ggcaaaatca gagttgaaag gcaatacgta ttcgcatgaa atcaaagtga acgttgtaag
    17101 aaatgcaaaa aatttgaatg tcgaaacgat tgaaacaggg atgtttgcca cgatttctta
    17161 taaaggaaag atatacaagt cggttttgac agcttggcga atatcaagtg ataaggagtt
    17221 tgtggaattg actttcggaa acataagaag tcgttttatg gattattttg aagataatgg
    17281 gggataaaaa atggttagca atgtggatgg atatcaattt gaaaacgtga aagtaagcgc
    17341 agaaaatgat gctagacttt atcacgtttt atataatcgg aaaaatcagg ttattgatgg
    17401 ttacgatcag tctatgaatt tatcttcaag cggattaaca gtaaaagttg ctgcaggagc
    17461 agcgattatt caaggtcgta tggtcgttgt tcgacaagaa gaaagtataa cagttccagc
    17521 aaactcaagt ggttatatag cattaacagt ggatttgaca caagaagtta tacctggatc
    17581 tattcttcca gaatcggaag aatatgaatg gactaataat caagtcaagc tagaatttat
    17641 aacaaaagtt ataaaaggta atttaaacaa tggtgataag gtctataatt taccactatg
    17701 ctcagttaat tctactggat caactgtttc aatctcaaag atatcggata gttacgagct
    17761 gactctctct aaaggagaaa ttttgtggag ggggactgcg ttaatgcatg atactcaaac
    17821 tgtccaacct tcaaaaaaaa tttggcagac agtttcaggt tttttgttag tatggctccc
    17881 atacgaaaac gggcaagcaa ttgaggatag atatgtaact acgccttttt ataaggagcg
    17941 tgtaactttt actaatgttt taggagaaat tgtttcagga tttgatgact atcacaaaaa
    18001 gtggtttagt aaacgaataa actataattc gaatactaat atatttacag gtgcagcaag
    18061 caatgcaagt ggagataacg caaatatggt gcttaggtat atagtttctt tttagttagg
    18121 aggtatggaa gagtggcaaa tttagaaatt aaattatctg caaataagag acagccttat
    18181 ctacgtcacc gtgttgttgg tagagttggt gatggggggc tcacaacaat caatgtacaa
    18241 cttcttgagg aagatgaaat tacacctttt gtaattaatc taaacggtac tttgaaattt
    18301 gtgggcgaag tttcaaacgg taactatacc gagggagaac cagaaataat cgattcgact
    18361 aatgggttaa ttagttacac gttcactaag tcaaatttca gcacgagtca tcaattcaaa
    18421 caagcatatt ttgaatatgt agatcctaac ggcaaaaaag taacttttca gaacttcatc
    18481 atagacgtgt tagaacgagt ggatattaat tcggagcaag cgaaatacta tatttcttca
    18541 ttggaaaaat tacagagtga aatgcaaacc actttcaatc agttcattag tgataaacag
    18601 gtccaatacg atcaaatcta ctcgaaatat aacgaattag taagattgat aaatgaatca
    18661 gataagcaag taaatgatcg tattgatcaa accaatcagc aaatcggcga tctcggcaag
    18721 ctgaaaaaga tgtacagtaa cagcatcgac tttgggggct atgattattc ggggaatccg
    18781 aatttgttaa gtaagctatc atacgactta attgaaaatc aaaatacttc agctggaaca
    18841 ctttctaagg gtgaaaactc gtttaaatat aataagatat cagctgaagt ggaaggtgga
    18901 gtagagttat attataaacg aagaggtata gctaactggt taccctctaa taaaacgctt
    18961 gtaatgactg ttaagcttag agctggagcg gactatagtc cagttgacgg aaaacttata
    19021 ctgattagat ataggtatgt tgacagtgga actggcaaga ttgttttaga cttacctatt
    19081 aacagtaatt cgataactca ggaatggaaa gagtttagta ttactggaac tactccaaca
    19141 tttagcccac aagcatacca tccttggata caatttaggg ctcaagatgg ggtacttggg
    19201 gaaatagaaa tgagctatga catcaaaatc gaagaaggct ctacagctac accatatcag
    19261 cctaacttac ttgatgactc ttactggcta ggtaaaacac cgttgggtga gaatttgata
    19321 acgaatgcca agttcccaat tataacacga agcaatccta tttctggttt tgatatttca
    19381 gaagaattaa taataggtga aacttatacg gtatctttaa aagggactaa gccagctact
    19441 aaagaattcc acttatacta cggagaagca aattaccagc agttgcagta tcaggcaact
    19501 ttacttcctg tggaaggatt aacagatgtg tggagtgcca cttttacagc cagaaatact
    19561 agtgaaacga caaatttact tagagtagcg ttatggcaaa aacctaattc tgcaacatat
    19621 gatactgttc aaattgactg gctcaagatt gaaaaaggcg acacacgcac accaaatatc
    19681 agtcagttta aatactttgg tgaaggcttg aaagacagta acaatccgaa cgactacagc
    19741 tgggatgtca caccagagta tactgaaaaa agcttgaata atacggttag tctgactgaa
    19801 ccgcaatcca tcgaaggatt gaaaaatttc gaagatggta ttcaatcaaa aggaaaatct
    19861 gtattgacat cagacgacaa caaatatgaa gtcgtaacct taacagttac aaacgggaat
    19921 accggatcag caaagcttta tcgtgaagga aaaaccgtca ctatttattt ttttgcgttg
    19981 aatgggaaaa gcagtggtgg aaatgattca acgatactaa caattccaga aggctatcgg
    20041 ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt gaacagtgct
    20101 cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag ttatggatcg
    20161 gattatacct ttgcaattac ttacacgatt tagaaaggcg tgaatcgata tgaaggcagc
    20221 atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt
    20281 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
    20341 ctgggaggga aataaatgaa aaacatttgg aaatatggcc gtactggcgg agagtatgca
    20401 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgctcgaa
    20461 ggggttcgtt cagatggcga accgctaacg attgctgatc agatgtttga tcctaaattg
    20521 aaccaatgga ttattttagc gaacgcgtta gatcacaacg atttaaacaa tctcaaagcg
    20581 atgtacgagg ctcttgaaca tgaaaacggc aacctaaaac agctaaatgc caaactcatg
    20641 ctaagcgatg tagcgattaa acaggaaaat actgcattga aagaaaaagc tgacagttta
    20701 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
    20761 attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga
    20821 tgtgaaaatg atgtatgatt ggggctgttt tactgacgat caagttcgtc tattcgttcc
    20881 actatgtatc acagatgaag aagcagatag aattattagc aaagaagaga gcgcatctta
    20941 agtgatgcgt ttttttgttg gaaagttggt ggaacatgaa agaagaagcg ctccaagacg
    21001 ttgtggagag attagtaaga attgaaacaa aattagacaa ctacgaatca cttagagaaa
    21061 aagctgatag tgcaaaagat ttggcagaca aagcctactc agtagcgcta aacaacgcag
    21121 aagatatcaa agaaatgaaa gccaataata aatggtcgtg gggttacatg atcggtttag
    21181 gcattacgat cattggctat ttcttgacta aattgtaaag gagttaagaa gaaatgattt
    21241 tacccgataa gtattatcaa gtcattaaat ggacggtttt aacagtatta ccagctgcat
    21301 ctgttttagt agccacgtta ggaaaagcgt atggatggaa tggaacagat atgacagtac
    21361 tcactatcaa tgcagtagca acatttttag gcgttatcac tggtgtgtct gcatataact
    21421 taaaaaaata ggaggaaaaa aatgaagaag aaaatcattt tatcattgag cctaataatg
    21481 gctctttttt tattgccaat taacgggttc gcctatacga tcaacaatga atttaattta
    21541 ggtgtaaatg aaggtagctc acaagtagca aataaccaat acatcctatt gcatgaaaca
    21601 gcaaacgaaa ctgcgacagg acgaaatgaa gcgcagtaca tgaaacgttc ttggtacaat
    21661 gcctatacag cgtatattgt tggcgatggt gggatcgttt accaagttgg acaacctggt
    21721 tatgtacaat atggcgctgg ttcatatgct aatgcaaata gtcctgtgca aatcgaactg
    21781 caacatacgc acgataaagt aacgtttgaa aagaactaca aggcatatgt tgaattagcg
    21841 agagattccg ctaataaata cggtattcca cttacattag acacaccgta taatcaacca
    21901 ggaatcaaat cacatttatg ggtaactcaa aacatttggg gcgatcacac tgatccgtat
    21961 ggatacttat cagaaatggg tgtaagtaag gaaaaactgg cttacgattt agctcatgga
    22021 tttacagacg aaaacccaac gacttctgag aacaagcctg tcattgatcc aacccgagca
    22081 ggtgcagcaa atccaacttt atcagatgga acgaaccatt ctcacattga tcagtttgga
    22141 gaaatcgaaa atgcgaactt acacgtcgct ggatggcaca tcgctaacta taaatacgag
    22201 tatattttca ttatggacta caatactggg aaagaattag ctcgagtaag agctgatgga
    22261 atttatagac cggatgtaaa tcaagcttat aatacttctg gaaacgttgg ctatcatgta
    22321 tcttttaaca tgcgcaattt ccctaataag aaagtatacg tcatgatgcg ggcaacgaat
    22381 gatccagaag gaaacactaa aggcggtgcg caagatttcc atgacaaacg ttggtattta
    22441 aatattccta aacgataaaa atagctcctc gttgaggagc agtacataga attgaaaatt
    22501 aacgttaaat caaaaaaata tttacaagat gtttatttaa gtagataata aaagaaaaaa
    22561 gcaggtgaaa ttgtgacagc agaaattggt attatgaata aaagtggaat cgtgttagct
    22621 tcagattcag catctacgat tggagatagc aaagtatata atactgcaaa aaaattattt
    22681 actttggatt ccatgcattc tgtaggtatc atgatatatg gtaatgctga atttaatggt
    22741 attccttggg aaataatcat tactcaatat aagaaaagca ttggtagttc tgtttttaat
    22801 actttggaag aatacgcgga taattttata gaatttgtca aaacagcatc ttttattaga
    22861 agtgaacaga ccgagcaaga acaaatgatt ggggtttttc aaaaaataat ttcaggttta
    22921 tttgagagta tagaagtaga tattaacttt ttaataagcc aaggtacaca aatagacaaa
    22981 gatgtcctag tgaagttatt acaaacaaaa atgaatacca atttgtctca acagtcccag
    23041 acattcattt tagatattga aaaggcgtta tttcttagta actatggaga aatattaaaa
    23101 gatattctta atagtatttc tacaatggaa ggtgtttcag aagctatatc agaggaaatt
    23161 caaagttacg tttatgaaat tataattaga gacgatgttt attcttcccc tacaggaata
    23221 gttatagcag gatatggaag aatggatatt ttccctaaac tatattctta taatatgttt
    23281 ggatttgtta tgaatattct gaaatactca gaatatgaaa gtgctcaaat aggaaatgac
    23341 aatggttcat taagatctac tatattacca tttgctcagt ctgatgtggt aaatactgta
    23401 gttcaagggg tagatccaca aattactaat tatctgtcga gtcaagt
//
LOCUS       DAZKCE010000041        23402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000041 DAZKCE010000000
VERSION     DAZKCE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.041"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1681
                     /locus_tag="ACG8KQ_001398"
     CDS             20..1681
                     /locus_tag="ACG8KQ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9104338.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1751..2098)
                     /locus_tag="ACG8KQ_001399"
     CDS             complement(1751..2098)
                     /locus_tag="ACG8KQ_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9104339.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2182..3123
                     /gene="opp3b"
                     /locus_tag="ACG8KQ_001400"
     CDS             2182..3123
                     /gene="opp3b"
                     /locus_tag="ACG8KQ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9104340.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3124..4167
                     /locus_tag="ACG8KQ_001401"
     CDS             3124..4167
                     /locus_tag="ACG8KQ_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104341.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4173..5243
                     /locus_tag="ACG8KQ_001402"
     CDS             4173..5243
                     /locus_tag="ACG8KQ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104342.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5233..6186
                     /locus_tag="ACG8KQ_001403"
     CDS             5233..6186
                     /locus_tag="ACG8KQ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104343.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6198..7208
                     /locus_tag="ACG8KQ_001404"
     CDS             6198..7208
                     /locus_tag="ACG8KQ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9104344.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7493..8314)
                     /locus_tag="ACG8KQ_001405"
     CDS             complement(7493..8314)
                     /locus_tag="ACG8KQ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9104345.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8411..8689)
                     /locus_tag="ACG8KQ_001406"
     CDS             complement(8411..8689)
                     /locus_tag="ACG8KQ_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104346.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8957..10966
                     /gene="metG"
                     /locus_tag="ACG8KQ_001407"
     CDS             8957..10966
                     /gene="metG"
                     /locus_tag="ACG8KQ_001407"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9104347.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11445..12032)
                     /locus_tag="ACG8KQ_001408"
     CDS             complement(11445..12032)
                     /locus_tag="ACG8KQ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104348.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12207..13529
                     /locus_tag="ACG8KQ_001409"
     CDS             12207..13529
                     /locus_tag="ACG8KQ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9104349.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13684..14460
                     /locus_tag="ACG8KQ_001410"
     CDS             13684..14460
                     /locus_tag="ACG8KQ_001410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9104350.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14453..15022
                     /gene="rnmV"
                     /locus_tag="ACG8KQ_001411"
     CDS             14453..15022
                     /gene="rnmV"
                     /locus_tag="ACG8KQ_001411"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9104351.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15015..15899
                     /gene="rsmA"
                     /locus_tag="ACG8KQ_001412"
     CDS             15015..15899
                     /gene="rsmA"
                     /locus_tag="ACG8KQ_001412"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9104352.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16350..16979
                     /locus_tag="ACG8KQ_001413"
     CDS             16350..16979
                     /locus_tag="ACG8KQ_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104353.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17103..18212)
                     /locus_tag="ACG8KQ_001414"
     CDS             complement(17103..18212)
                     /locus_tag="ACG8KQ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104354.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18421..18843
                     /gene="mgsA"
                     /locus_tag="ACG8KQ_001415"
     CDS             18421..18843
                     /gene="mgsA"
                     /locus_tag="ACG8KQ_001415"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9104355.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19030..19128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19166..19702
                     /locus_tag="ACG8KQ_001416"
     CDS             19166..19702
                     /locus_tag="ACG8KQ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9104356.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19742..21505)
                     /locus_tag="ACG8KQ_001417"
     CDS             complement(19742..21505)
                     /locus_tag="ACG8KQ_001417"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104357.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21508..23223)
                     /locus_tag="ACG8KQ_001418"
     CDS             complement(21508..23223)
                     /locus_tag="ACG8KQ_001418"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104358.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7538 a         4286 c         4770 g         6808 t
ORIGIN      
        1 caataaaggg gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg
       61 ccttgtactt gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag
      121 cggttcagca gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc
      181 tgacttatca gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg
      241 aatttaccgt cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga
      301 agtcagtgaa gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa
      361 tggtgatcct gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc
      421 tacagcttct gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc
      481 tggaaagaaa gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat
      541 caaattgacg aaacaaacac catatttcca atatctgctt gctttcccat catttttccc
      601 acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc
      661 tgtttataat ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg
      721 gacttataag aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa
      781 agtcagtgta gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga
      841 tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc
      901 tgttaaagaa gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt
      961 caacaatgtg aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa
     1021 acaagtacta ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa
     1081 aagtccaaca acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa
     1141 agataaagca aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga
     1201 atttgatttg atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa
     1261 ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt
     1321 acgtcttgac cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga
     1381 ctatgcagac ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg
     1441 tcgctggagc aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg
     1501 cgatgaacaa gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat
     1561 gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg
     1621 tatcgttcac catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata
     1681 atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc
     1741 tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta
     1801 ttccggcaat gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa
     1861 attctggttt tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat
     1921 cgaagaaacc cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc
     1981 ataagaatag atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta
     2041 ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt
     2101 gattgctcct ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc
     2161 aaaagctagg gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt
     2221 ttcatgatca tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg
     2281 ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat
     2341 aaacaagtcg gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg
     2401 cttcaaggag attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta
     2461 gctggaagaa tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc
     2521 ctagggacga ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct
     2581 tctacactcg tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta
     2641 caatatatct ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg
     2701 tacacaatct taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt
     2761 atccgtacgg aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa
     2821 ggattgagcc gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg
     2881 atgactttat taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat
     2941 atctttgcta ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat
     3001 cctacgatca tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc
     3061 gtggatctat tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga
     3121 taaatggaaa tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt
     3181 cttccattac aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat
     3241 tttatccaag attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt
     3301 ttactattgg tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca
     3361 actgcccaaa acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat
     3421 ggattgaatg gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac
     3481 gtcccttcaa acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc
     3541 cgcttattta tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat
     3601 gtaacgattg gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac
     3661 gtgatgcaac gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg
     3721 atgttggtcg tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac
     3781 tggataccaa tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat
     3841 gttttagctg gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg
     3901 ccgaatatct ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc
     3961 ttcgaagcct ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga
     4021 acgatgttga gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg
     4081 gcagtaactt tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat
     4141 gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact
     4201 tagaaatctc ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc
     4261 atttgaataa aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga
     4321 cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc
     4381 tctttaaagg taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta
     4441 aagaaatagc gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg
     4501 ggaagcaagt cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa
     4561 aagctgcatt agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca
     4621 gttatccgca tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg
     4681 tatgttatcc ggaaatactg attgctgatg aacctacaac agcgcttgat gtgactattc
     4741 aagcacagat tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct
     4801 ttatcactca tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg
     4861 gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct
     4921 atacttgggg actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg
     4981 ctattcctgg ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa
     5041 gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa
     5101 aaacacacaa ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag
     5161 aagaaatcca acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag
     5221 gaggaattgc tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat
     5281 gtaggaaaaa agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga
     5341 gaaacattcg gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc
     5401 attcgcttga atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag
     5461 atcaaagata aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat
     5521 ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat
     5581 gttaatggac tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga
     5641 actgttggat tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa
     5701 cgccagcgta ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat
     5761 gaaccaattt ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat
     5821 ctccaaaaag aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa
     5881 catattagtg accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt
     5941 gatgaaatct ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct
     6001 ctaccagatc cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat
     6061 gacggacaag tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag
     6121 caggaagtcc catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct
     6181 aactaggagg gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac
     6241 aaaaacagca attgattcgt acttttggat gtgctcgatt tacctataac catttgctga
     6301 aaaagcggca gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta
     6361 cattaaaaaa agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac
     6421 agcttaattt ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc
     6481 ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct
     6541 atctggaaaa tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc
     6601 atcggccagt tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat
     6661 tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga
     6721 tcgggatcgc ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt
     6781 taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga
     6841 aagtccgctc cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac
     6901 aaaagagaaa agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg
     6961 aacaattatc tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa
     7021 aagaagaggc acatgctgat ttttctattc acgactggca taaattaata acaaaattgc
     7081 gttataagtc ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag
     7141 aaagcaattc cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca
     7201 agggataaaa aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt
     7261 cttaatgact tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa
     7321 accaataaaa caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt
     7381 attcagaaag tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt
     7441 tgtttttgcg ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat
     7501 ggtgccccgg cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac
     7561 cgatctcatc gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat
     7621 aatcgatccc taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac
     7681 ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt
     7741 tcaagatgat ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga
     7801 ctaaatcaag cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt
     7861 ctagtgtgaa ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa
     7921 taaaggctaa gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga
     7981 atgtatgcag ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt
     8041 ctcttaagac gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc
     8101 atgaatttcg ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt
     8161 ctttgttata gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac
     8221 gttcctgttt ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata
     8281 atttttcttc ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag
     8341 tactttttac cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct
     8401 aggttatcat ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca
     8461 gataaaacgg caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga
     8521 tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc
     8581 catggaggtt aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta
     8641 atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag
     8701 tcccttacta agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg
     8761 ttttttagta gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt
     8821 aaaggataga ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa
     8881 gctaacagca tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata
     8941 aaggaggaag agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta
     9001 tccaagcggc aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc
     9061 tcgttataaa cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg
     9121 tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa
     9181 aatggcggct gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat
     9241 tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga
     9301 acagggagat atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt
     9361 ctttacagaa acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg
     9421 taaagcgcca agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat
     9481 gagcaaatac gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc
     9541 agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc
     9601 agtttcacga acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt
     9661 gtatgtatgg atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga
     9721 tgaaagtcta ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt
     9781 tcgctttcat acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa
     9841 aaaagtattt gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg
     9901 aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta
     9961 tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc
    10021 ccgtgtcaat tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat
    10081 gatcaacaaa tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga
    10141 tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa
    10201 aatggaattc aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata
    10261 cattgatgaa acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa
    10321 tagtgtcatg atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat
    10381 catgacagaa acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa
    10441 tcttgaagga ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac
    10501 accaatcttc ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc
    10561 tgaaggtact caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt
    10621 ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc
    10681 agaagtcatc aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga
    10741 tgctggagac agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc
    10801 aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg
    10861 tggacaaatc agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt
    10921 aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg
    10981 tatgacacta gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat
    11041 tccttcttat tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa
    11101 tcctagaaaa agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg
    11161 aatcgtaaaa acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca
    11221 tctggtactc ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac
    11281 atctatattt atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg
    11341 ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg
    11401 gctttgactt cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg
    11461 taacctttta tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg
    11521 atcgaaaaga cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc
    11581 aacatcacgt gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta
    11641 tcccaatgca tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc
    11701 ccgactaagg tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc
    11761 gctgaaagca aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc
    11821 cacaagatca tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat
    11881 attagttttt gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa
    11941 tgccaatggg actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct
    12001 gctaatttcg gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta
    12061 ggtttagtat acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg
    12121 aaagtggaga aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca
    12181 aatttcaaag aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt
    12241 ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg
    12301 ctgctgttac tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg
    12361 gtttcttaac gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa
    12421 ctggtggagc tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag
    12481 gatgggcgca aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg
    12541 cgacacaatt gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga
    12601 tcaccgctgt ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac
    12661 aatcagtgac gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat
    12721 tgaccccagg acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt
    12781 ttgtcgaagg attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag
    12841 gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta
    12901 ttcttggttt gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa
    12961 ctttgccgat tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca
    13021 tttttggagg aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg
    13081 cattgaacgg ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg
    13141 agctaccttt cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc
    13201 ctgcggtttt ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt
    13261 taacagacat gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct
    13321 ttattttaag aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa
    13381 tcgttccgat tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc
    13441 aaacgaaatt agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc
    13501 aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga
    13561 actaggtcct ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg
    13621 atttttgcta cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata
    13681 aacatgatat ttgattctca tacccattta aatgctgaac agttcaatga agatattcca
    13741 gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat
    13801 acgccaacga ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt
    13861 atcggctggc atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa
    13921 gaacaattga ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg
    13981 atggaagacc cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa
    14041 gaaatgaacc taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc
    14101 ctaaaagaag aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat
    14161 gaatgggcaa aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact
    14221 ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt
    14281 gtggaaacag atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca
    14341 ggctatactc gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa
    14401 gtggctgccc aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa
    14461 gatgaagatc caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga
    14521 agtagttgat gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat
    14581 gcaaattgaa catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc
    14641 aggagaaaaa ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt
    14701 gccgcgcaca caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga
    14761 tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt
    14821 ttctgaaatc actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa
    14881 agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt
    14941 ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat
    15001 agtggaggaa caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga
    15061 ttttattgaa acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac
    15121 caaatatttt aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg
    15181 aagttggacc aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg
    15241 tagcatttga gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg
    15301 acaatgtgac tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag
    15361 aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga
    15421 caccgatcat gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga
    15481 tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat
    15541 tatcgattgc tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag
    15601 tatttgtccc tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac
    15661 cggcagtcga agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc
    15721 aaagaagaaa aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa
    15781 aagcatggtt agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga
    15841 ctttgtcatt acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa
    15901 aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
    15961 aaacaaaaat agttcctcat gtttttcgtt tgtttgggct tattaccgca gatgcaagct
    16021 tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa
    16081 caatttcaaa aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag
    16141 cttttgacca ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat
    16201 ttttgtttct tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt
    16261 agaaaagaat atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat
    16321 gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga
    16381 tttctgcttt attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga
    16441 ttccaccagc ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta
    16501 caccaggagt tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc
    16561 cggttatcat cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt
    16621 acttgcaaaa gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc
    16681 gtttccaagg gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag
    16741 tcagcatctt ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt
    16801 atacagtatt catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata
    16861 ttgcttattt tatagcagga acactcagca aatattttga tatccccgta tttacggcag
    16921 caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga
    16981 catgatctct caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag
    17041 agttttttgt cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca
    17101 atttatggaa gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg
    17161 ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag
    17221 atgaactcag tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa
    17281 acgacaactt ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg
    17341 tggatgtctt ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact
    17401 aatactttgt tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca
    17461 ccatttttca atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg
    17521 aacatattga cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg
    17581 tctttcatga taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca
    17641 tagatggtcg ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca
    17701 cgtaatttag catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga
    17761 acgattgccc gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt
    17821 cgtttcaagt attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg
    17881 tctgctttat catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata
    17941 tgaggataca atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg
    18001 tcattcatga cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg
    18061 atcattcgca aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca
    18121 cggtctttga ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt
    18181 ttatatacgt ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt
    18241 ttattaacta tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa
    18301 gaattttttc ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa
    18361 ataaaaactg atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt
    18421 atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca
    18481 gcgtataaaa caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg
    18541 atcacggaag caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat
    18601 cagcaaattg gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac
    18661 ccgctggctg cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc
    18721 tatgagattc cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa
    18781 gccggcttcc tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt
    18841 tgatggataa gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc
    18901 taaacaaact gataatatgc gatataatac gaacgtattg gataatataa acaataatat
    18961 tagtttttcg tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc
    19021 caaaaataat acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc
    19081 ttggacgaag gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta
    19141 gcagctctta aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc
    19201 tattatgggc ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac
    19261 cccatttatt aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg
    19321 tcctttttgg tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc
    19381 gaaatctgca ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg
    19441 attctttttc tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt
    19501 cacgctgatc atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc
    19561 attgttcagc tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat
    19621 tcaatcaatt gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag
    19681 tttagcgatc cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt
    19741 tttattgtgc tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca
    19801 gctcatcatg ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg
    19861 catttttgat tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg
    19921 ctaagaaggc ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat
    19981 ctaagatcaa cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt
    20041 gtccatctga tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac
    20101 gttctacaaa ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg
    20161 cttccggatt cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt
    20221 gtaacaccat tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt
    20281 catcgactgt aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg
    20341 tggattttcc ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa
    20401 aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag
    20461 agacttttcc tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt
    20521 cttctggctg atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt
    20581 gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa
    20641 agctggaaat cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa
    20701 ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg
    20761 aagaagaaaa ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt
    20821 gctgttgact agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt
    20881 cggtcacaaa gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg
    20941 aagcacgagc aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca
    21001 atgtcagaag tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc
    21061 cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg
    21121 taaatcgact gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat
    21181 tcagatgagc aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc
    21241 ttaacacttg gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa
    21301 taagtaaatg gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac
    21361 tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca
    21421 cagctagcca aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct
    21481 gcaaggataa tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg
    21541 atattctgct gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg
    21601 atccatcact aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat
    21661 cactgtactg ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc
    21721 caacgcactt aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc
    21781 aatcgtcagt cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt
    21841 atctaaacca tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc
    21901 ccaacattct ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc
    21961 ggtaaatagt acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg
    22021 ccaagaacga acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg
    22081 cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac
    22141 tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata
    22201 acggaagtca acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact
    22261 gacctcagct tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga
    22321 agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat
    22381 ttgattcata taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac
    22441 tttgatcccg ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc
    22501 aggtgataat agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt
    22561 gcttttatcg aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac
    22621 accgctaaga ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg
    22681 gtagagagga acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa
    22741 tgctaagaaa aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat
    22801 acttaaaaac ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg
    22861 attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt
    22921 gtgagagaaa gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg
    22981 tgaagctact gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat
    23041 agcgaccatc cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt
    23101 atcgatcaag gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt
    23161 gaagaacggg cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat
    23221 catatgattc ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg
    23281 tagcttagga aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta
    23341 tgaagaagct ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca
    23401 tg
//
LOCUS       DAZKCE010000042        22737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000042 DAZKCE010000000
VERSION     DAZKCE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.042"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..418)
                     /locus_tag="ACG8KQ_001419"
     CDS             complement(<1..418)
                     /locus_tag="ACG8KQ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9104359.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNK"
     gene            complement(509..961)
                     /locus_tag="ACG8KQ_001420"
     CDS             complement(509..961)
                     /locus_tag="ACG8KQ_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9104360.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(974..1384)
                     /locus_tag="ACG8KQ_001421"
     CDS             complement(974..1384)
                     /locus_tag="ACG8KQ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9104361.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1659..1871
                     /locus_tag="ACG8KQ_001422"
     CDS             1659..1871
                     /locus_tag="ACG8KQ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFP9104362.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1874..2086
                     /locus_tag="ACG8KQ_001423"
     CDS             1874..2086
                     /locus_tag="ACG8KQ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104363.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2226..2381
                     /locus_tag="ACG8KQ_001424"
     CDS             2226..2381
                     /locus_tag="ACG8KQ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104364.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2378..2602)
                     /locus_tag="ACG8KQ_001425"
     CDS             complement(2378..2602)
                     /locus_tag="ACG8KQ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9104365.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2680..2868
                     /locus_tag="ACG8KQ_001426"
     CDS             2680..2868
                     /locus_tag="ACG8KQ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104366.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2865..3038
                     /locus_tag="ACG8KQ_001427"
     CDS             2865..3038
                     /locus_tag="ACG8KQ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104367.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            3007..3135
                     /locus_tag="ACG8KQ_001428"
     CDS             3007..3135
                     /locus_tag="ACG8KQ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104368.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3194..3529
                     /locus_tag="ACG8KQ_001429"
     CDS             3194..3529
                     /locus_tag="ACG8KQ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104369.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3762..4703
                     /locus_tag="ACG8KQ_001430"
     CDS             3762..4703
                     /locus_tag="ACG8KQ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFP9104370.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4705..5595
                     /locus_tag="ACG8KQ_001431"
     CDS             4705..5595
                     /locus_tag="ACG8KQ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFP9104371.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5638..6438
                     /locus_tag="ACG8KQ_001432"
     CDS             5638..6438
                     /locus_tag="ACG8KQ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9104372.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6453..7304
                     /locus_tag="ACG8KQ_001433"
     CDS             6453..7304
                     /locus_tag="ACG8KQ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9104373.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7370..7504
                     /locus_tag="ACG8KQ_001434"
     CDS             7370..7504
                     /locus_tag="ACG8KQ_001434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104374.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7505..7966
                     /locus_tag="ACG8KQ_001435"
     CDS             7505..7966
                     /locus_tag="ACG8KQ_001435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9104375.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7992..8444
                     /locus_tag="ACG8KQ_001436"
     CDS             7992..8444
                     /locus_tag="ACG8KQ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104376.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8550..8744
                     /locus_tag="ACG8KQ_001437"
     CDS             8550..8744
                     /locus_tag="ACG8KQ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104377.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8775..9068
                     /locus_tag="ACG8KQ_001438"
     CDS             8775..9068
                     /locus_tag="ACG8KQ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104378.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8999..9538
                     /locus_tag="ACG8KQ_001439"
     CDS             8999..9538
                     /locus_tag="ACG8KQ_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9104379.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9613..10080
                     /locus_tag="ACG8KQ_001440"
     CDS             9613..10080
                     /locus_tag="ACG8KQ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFP9104380.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10827..11057
                     /locus_tag="ACG8KQ_001441"
     CDS             10827..11057
                     /locus_tag="ACG8KQ_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104381.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11216..12049
                     /gene="terS"
                     /locus_tag="ACG8KQ_001442"
     CDS             11216..12049
                     /gene="terS"
                     /locus_tag="ACG8KQ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9104382.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12042..13457
                     /locus_tag="ACG8KQ_001443"
     CDS             12042..13457
                     /locus_tag="ACG8KQ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9104383.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13469..14998
                     /locus_tag="ACG8KQ_001444"
     CDS             13469..14998
                     /locus_tag="ACG8KQ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9104384.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14916..15968
                     /locus_tag="ACG8KQ_001445"
     CDS             14916..15968
                     /locus_tag="ACG8KQ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFP9104385.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15970..16287
                     /locus_tag="ACG8KQ_001446"
     CDS             15970..16287
                     /locus_tag="ACG8KQ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104386.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16414..17037
                     /locus_tag="ACG8KQ_001447"
     CDS             16414..17037
                     /locus_tag="ACG8KQ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFP9104387.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17052..17942
                     /locus_tag="ACG8KQ_001448"
     CDS             17052..17942
                     /locus_tag="ACG8KQ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFP9104388.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17965..18180
                     /locus_tag="ACG8KQ_001449"
     CDS             17965..18180
                     /locus_tag="ACG8KQ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104389.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18192..18533
                     /locus_tag="ACG8KQ_001450"
     CDS             18192..18533
                     /locus_tag="ACG8KQ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104390.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18533..18904
                     /locus_tag="ACG8KQ_001451"
     CDS             18533..18904
                     /locus_tag="ACG8KQ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104391.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18897..19292
                     /locus_tag="ACG8KQ_001452"
     CDS             18897..19292
                     /locus_tag="ACG8KQ_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFP9104392.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19294..19671
                     /locus_tag="ACG8KQ_001453"
     CDS             19294..19671
                     /locus_tag="ACG8KQ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFP9104393.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19672..20280
                     /locus_tag="ACG8KQ_001454"
     CDS             19672..20280
                     /locus_tag="ACG8KQ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9104394.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20280..20621
                     /locus_tag="ACG8KQ_001455"
     CDS             20280..20621
                     /locus_tag="ACG8KQ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104395.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20836..>22737
                     /locus_tag="ACG8KQ_001456"
     CDS             20836..>22737
                     /locus_tag="ACG8KQ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9104396.1"
                     /translation="MNNDDLILKMILDESGFTAGMNNAVKKLNSFDETIERKSRNSGS
                     SLGSIWKIFAGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNS
                     EIMKNIGKNGLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFG
                     GSTDQVNEAVIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLS
                     QGKISVEEFQNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIE
                     GLNKALVDSDLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALK
                     FMMPFLVPAATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVL
                     IIGAVVGAIAVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEM
                     ASNAVEGVKDAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKW
                     FSDTWQSIKDSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKN
                     VFEILKNVILAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFT
                     NIYFAALNIW"
BASE COUNT         8148 a         3332 c         4633 g         6624 t
ORIGIN      
        1 gtttatttaa aataagaggg acagaaacag catcgtcaat aatatattcc ttttctttta
       61 attttaacaa agaaagctta ttttttattt cagaagatga agtttcttct atatttgaaa
      121 cgataagtga agaatagaca ttttttaatg tttcatcata cttttgattt atttcatcat
      181 ttaaatcttc tttctctttt tttaataaaa taatatcatt ttttaacata gtaatttgtt
      241 tatttaaatc tgatagtgtt tcttccttac ttaatataaa tctttctcta tcatgtaatt
      301 tatttagttt ttcatcattt ttttgttcta attcataagc ttcttttttt attgtgttag
      361 ctttcgacag tttagtgatt cctataatta ataaaataag tccaatcaat aataacattc
      421 aaaactcctc atttctttga tataattatt gtagagttta ctcagaaatg aggaaagagt
      481 ccatgtgaaa gcatgggctt ttttattttt aatgttgtaa tactaaatgt tcaagattat
      541 gaggaagccc taaatattca ataatttgga actgactcat atcagataaa tcttgttcat
      601 ctattgttct agttaacatt tcaattgcaa acatattagc ttcagcttct ataccataaa
      661 tcatacaact aaggttgttt gctctaaaag ctgctgtgca aataccttta tggagtctaa
      721 cgtgggctat ttcatgccat aatacaaatc ttttcataga aggtgatagg ttttgattaa
      781 ttaatatcac ataacatctt ttattgtgta tcgtttgtcc taatgtttta ttccctaaat
      841 cagtttcaat taaatctata tcagaatatt tgattatttt atatggatta atcgcatcag
      901 atccaaactc tacaaataat ttttctattt cttctaatat ccagtatttg tttaaatgca
      961 taccaaagcc cacctattga cgatattttt taggagtgaa ttttttcttt gctttaactt
     1021 tggttaaggc aagtgcaagt tgaatggcat cgtataattt ttccttatct tcatcgtcta
     1081 aaggttcacc ataataattt gtttcagcat ctgaattcaa gccttcaagt aatttttcag
     1141 cttgtaaagc aatatcttgt ttatcttttt ctgttaaatc ataataatgg cgtttatcag
     1201 tacgtcctaa aagataatca gttgatacat taaagtaatc tgcaactttt tgtattttat
     1261 ctccagatgg agtactttta ttccatctag acatgcttcc atttccgaaa tttaactttc
     1321 tttcaagttc tgccaacgtc attgaacgtt cttttaataa gatttttata cggtctaata
     1381 gcataggata tgcattcctt tcaaaatacc gacaaaaaaa gtatttttct gcaaattatc
     1441 tatttacaaa tagagaattt gcagttatac tagttttgta agcaagatat ttacggcata
     1501 caaagactaa aagcaataaa ctaatagtct ccccagacat cagtaagctc gctttttgtg
     1561 cgctgtattt tgtatgctta cataatagca tatttgcagt ttttgtcaaa cttattagac
     1621 aaaaatatta gaaaatattc gataaggggg tatgaagcat gactttaaaa cagcaagtat
     1681 taattgcttt agtaaagaaa ggttggagtc aaagagagtt agctcgacga atgggtatat
     1741 caattactta tttacgagat atcttcatcg aaaaaagaaa acctaaggaa cgtttaaaac
     1801 aaatcgaaga acttttggat atcaagttag aagtagattc taatgatcag ccatcagaac
     1861 aggaggcgta aaaatggtac aacgtttgag caaagcggag tttaaagaga aatataagta
     1921 ttctgaaagt acttatcaaa gaagaatgaa ggaatttaaa aaatcacgtt ttagtgaggg
     1981 gtataacgca gttacttcca aagaaataac aattgatgtc gatttatatg aacagtttag
     2041 aacatggcgt tctaaaaatc gatttagagt tagagctgtt tgttaatagc taaaaaatct
     2101 aaattcacca cttctgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac
     2161 gttttttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
     2221 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
     2281 ttattgaaac agaagaaggg aaaaaagtag cagaagttac attgaccgat gcaactcctg
     2341 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
     2401 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
     2461 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
     2521 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
     2581 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
     2641 tagaaatgga gaaattcaat aaacgaaagg agaagtgata tggcatatac cttgcaacaa
     2701 gaacatcaaa ttctcggttt gattaaacaa cgcaggaaac aattacaaga tgaccgtgca
     2761 gcgcttagaa aagccgatga gctatcagat aggcaagctg aactaattgc ttctgaactt
     2821 gaggatttga gaatgctaga aataaaaaat agggagatta gattatgaag aagacagaca
     2881 cactttttat aggattcatt ttggggttat tagtgattgt agcgcaccaa agtattatcg
     2941 ggggaagctt gttcgcagca ttgatggttt taatcaatct gcttgattca aaagaaagga
     3001 gcaactatgg cacgagaaga agcgctaaaa atcggtaaag tgattgctga taatcggtgg
     3061 ggaaatagcc gccctattat tttaagcaag caacatatcg aaaagcaaaa agcatggcaa
     3121 caaataaaaa agtgactctg ccagccagca aagagtcaca tacaaattta taaaactagg
     3181 agaattatat cacatgaata agaaaattga aaatcttatc gaagaattga aacgtgaatg
     3241 ccaaaagcaa ggtgtttcta ttatttgtac agcccaaaaa gaaggggaac ttaaaagtct
     3301 cgtttatggt gagacaacag aaattttact ttgtcttgct atgcaagaag aacatttaga
     3361 tgaaaatctc ccattgtcag cacatattat gcgtagaatt gctgtggatg cttacgaaca
     3421 agcgaaaaat gaagaagaaa atcaaccttc aaatcatact tttgttatta ataacaagga
     3481 agatttagct gatgtgatga ctcgtattct caagggggaa tttcaatgat gaatgaaaaa
     3541 acaattgatc tactcattat tttatttttc acgttagtga ttgtagcgaa aagttatgta
     3601 atctttggct tgttattcgc aattaccatg cttgtgcttt gcgtgttagc aagcagaaaa
     3661 gaggttaaac atgaacgagt tagcaaatct tgacaactat ttaactgatc ctgattatgc
     3721 taaaccacca tatgaagcac caattgatga ggaggatgaa gatgagtaaa tctaccttag
     3781 aaatgagcca tcaagaatgg cttgaagacc gtaagaaagg catcggaggt tcggatgttg
     3841 gaacaatttt aggattgaat aaatggaaat ctccttatca actatggcta gaaaaaacag
     3901 gacaagtagt acttgaagaa tcaggaagtg agcccgctta ttggggtaat gttttagaag
     3961 aagtggttgc taaagagttt caggaacgta caggcaaaaa agtacgcaga agaaaccaag
     4021 tatttgaaca tccattacat ccatttttaa gagcaaatat tgatcgggaa gtagtgggag
     4081 aaaatgccat tctggaatgc aaaacagcca atcaatttct cggtaaagaa tgggaaggcg
     4141 aagaggtacc gcttagctat ctctgtcaag ttcaacatta catgaacgtt ctaaacaaag
     4201 actattgtta catcgctgtc ttaatcggtg gtcaaaaatt tatctggaag cggattgaac
     4261 gagatcaaga gctgatcgat acaatcactg aacaattagt agagttttgg gaaacaaacg
     4321 ttcttggagg tatcgagcca attattgatg gtagtcaagc gacagctgat ttcttgaaag
     4381 agaagtatgc agatgttgaa gacgttcaaa cgacgttacc aattcatttc gatgaactag
     4441 ttgaacagaa aaacgaactc aaacggacta agaaagaaat tgaatcagct atccgtcaag
     4501 tagacaacga gattatcagc gagctaggta aacgtgaagc cagtatcggt atcactcaaa
     4561 aaaacatcat cagttggaaa tttgtccgta cgagacgtat gaactcgaag aaactagcag
     4621 agaaatatcc agatgtcgca aatgatgaag agatttataa cgttactgaa tcaagaaggc
     4681 taaccgaaag ggagatcaaa taatatggca acaaatgaat cgttaaaaaa tcaattggca
     4741 gcaaagccac agaaacaagt tgcaccaggt caattaggac ttaaagcttt gatgaataca
     4801 ccaacaatga gaaagaagtt cgaagaggtg cttcatgaca atgctaatgc ttttatgtcg
     4861 aatgttatga ctcttgtatc taatgacagt tatcttgcag atagtgaacc gatgtctatc
     4921 atgagtggtg cgttaactgc tgcaacatta aatcttgggc tagataagaa tttaggttat
     4981 gcatatttag ttccattcaa tagtaaaaac aagcaaacag gaaaatggga aaagaaagct
     5041 caattcatgc ttggctataa aggatatatc caattagccc aacgatcagg taaatacaaa
     5101 gcattaaatg tgatcgaagt ttacgaagga gaactaaaaa gctggaaccg actgacagaa
     5161 gagtttgagt ttgatccaaa tggtagaacg tctgatgaag tcattggata tgttggctat
     5221 ttcgagttac tgaatggatt caaaaaaact gtctattgga ccaaacaaga aatcgaagct
     5281 catcggattg ctaacaataa agatcgcgat aagacaaagt taagtggtgt gtgggcatct
     5341 gattacaatg caatggcacg aaaaactgtt ttgagaaatc ttctttctaa atggggaatc
     5401 ttgtccatcg aaatgcaaga agccaccaca tcggatgaga gagtccaaag ggttcaagaa
     5461 gacggcagca ttattgctga aacagaagtt gaagaagata ttcctgaaag aaaagaagca
     5521 gaggttatat ctgaagaaaa tgaagatgta caaactggat tatttgatgc atctaatccg
     5581 ccgttaaaca aataatgagg gagctttctc ccttgctttt ctagaggaga aatacgaatg
     5641 aacacaggat atgtcaaatt acatcgaaaa gtgatgaatt cattcgtttg gaccaatcct
     5701 tatatgtata aattatggaa cttgtgtttg atgaaagcga gtcatgagaa tcgcaagata
     5761 ctttttaatg gaaaagagat acaagtgaac agcggagaat tcgtcacagg gcgcgatgct
     5821 atcacatctg agatgaacga aggcgtgaaa cgtgaacatc aagtgaacag cggttctgta
     5881 tggagatggc taaaacaatt tgaaaaaaac ggaatgttga acatcaaatc aactacgaaa
     5941 tacagcgtta tatcaataaa aaactggtct ttgtaccaaa gcagtgaaca acaaatgaac
     6001 atcaaacgca caacaagtga acaacaagtg cacacaaaca agaatgaaaa gaattataag
     6061 aatgaaaaga ataataaaac atcatcgtcg caaccacgca aaaagcgtgt ttacgacacc
     6121 gactcagttt actacattct cgcggaggag ttattcaaac agatttgtca gaatcaggaa
     6181 atcaaaaagc cgaatctgca aagttgggca gatgacattc ggaaaatgat cgagatcgac
     6241 aagcggaccg aaaaccaggt acggggaatg attgaatgga gtcagcacaa tgtgttttgg
     6301 gcatcgaata ttttatctgc taaaaaattg cgagaaaaat acgacacaat ggcagcgcaa
     6361 gcaaatcgtg attataaaac aaaacaaact aaaacgcttg aatacgagaa atttagcaca
     6421 gatgagttgc ctatttgaga ggaggcgtag gcatggaaac tgttggggaa atcatggaaa
     6481 agctgataca gaaagtgctt gtccaacgtg gcgaatgtcc tgaatgcgga cagcctttgt
     6541 atggatggcg tacgaagaac cctgatggct cagaacgttg taagccaacc tgtatgcaat
     6601 gtgggtataa agcattgcga gttcaggaag acttacaaac agaacgaatt tacaacgaga
     6661 gcctgaaagc aagagcaatc aattttttca aaggtggttc tgttgtgcct aatcaagcgt
     6721 tgtttgattg cacattgcag aattatcaaa ttgtcgatca agaaacaaga caagcggttg
     6781 aagtaaccaa acgctttgtt aattcagtct tgttaggaaa tccaagtcac cttgttttaa
     6841 ctggaaaaca aggaacaggt aaaagtcacc tagcgatggc agcggcttgg gaagtcttga
     6901 agcgatcaaa ctacgataaa aaaatcttat ttatcgggtt acaggaaatg ctggatcaaa
     6961 tcaaattttc gtacaacaat cctgaactca gaaaaacgat tgagggatcg ttgattgcag
     7021 atatcaaaac agcggattta gtcatcattg atgatatcgg ttcagaacta ggaaaagatg
     7081 catcagatag tcgagcgttt ggcataaaca cgctaaattc gttcttggat gcacgacaga
     7141 acctagcaac gattatcaca acgaacttgc ttggtgaaga actgaaaaaa gcttacggta
     7201 cgagaacaat atcaagaatg tttgtcaact ctgatggatt tacgatggta ttttctcaaa
     7261 cagcagacaa gcgcataaaa ccagtcaaag gagcgatcgc atgaatttga ctaaacaaga
     7321 aaaaagaatc tgtcgtcaga taatgttgca aattataggt gatcgtgaca tggacgaatt
     7381 atctcaacaa gagagtcgaa tggttcggaa gataagtaag ctaattattt atcgggatac
     7441 agaaagattt ctcactgttt taggtaacgt ctacgagaat ccagagttat tggaggtaga
     7501 gtaattgcca aaaatattag atgcatgttg cggtagtaga ttgttctggt ttgataagaa
     7561 caattctgac gtaacattta tggattgtcg tcagcagtat gaagaattat caacaggtca
     7621 tgtgatcaat gttgatccag atgtggtcgc agactttcgc gatatgcctt ttgaagatga
     7681 tgtgttcgat atggttgtat ttgacccgcc acacttaaga catgtcggcg aaaactcatg
     7741 gcttgccaaa aaatatggaa aattagacga actttggcca gaagacatca ggcaaggttt
     7801 tgcagagtgt atgcgagttt tacgaccttc aggaacatta atttttaagt ggaacgagga
     7861 acagatacca ctttctgatg tgttagaggc tattggagaa cagccattgt ttggtaataa
     7921 acgaagcaaa acacattggc ttgtgtttat gaaaaaattc agctaacgga ggaaacaaag
     7981 gaggaaaagt aatgaagatt gtattaaata aatgttttgg tggctttggg ttgagccatg
     8041 aagcaaaaat ggaaattttt aaaagaaaaa atattgaagt attcccatac atcaataact
     8101 ttagctatga ctcagatgat gaatacacga gatacacagg ccaaaagcta ggttcgatgg
     8161 attttatcta ctatttcaaa aaagacccga aaatcgacaa agttacagga atttatagcg
     8221 aaattgaccg actgtacgga attgctgatg attccagttt ttcaagtgat tcaaacagag
     8281 gtgataagga cctcgttgct gttgtcgaaa aactaggaag tgaggccagt ggaccatatg
     8341 caagtcttaa agtcgttgat atacccgatg gggctgagtg ggaaataagt gattatgacg
     8401 gcgttgaaac tgcacattat ggttttcaaa caggtagtat ttagtccact aatgacaact
     8461 aaagaggagg cagaaggatg agtgaagatg aatactttga acagtagtct attaacgaca
     8521 gagattttta actggaaagg ggtgtagtca tggacgcatc ggaagtagta aataaaatta
     8581 atagtctcaa agaggttttc ggagacgtcg aagtagtgat aaacgcacaa ggctatgaca
     8641 acgaattttt taagcagatt accgacatta gcattcagca aggaatggaa gacgaagacg
     8701 gaaactttat cgatgaagta gccattttag tgacttgcga gtaagtaatt gacagctaaa
     8761 gaggaggaag cggaatgaaa ctaaaagacg gattttactc cagcggccac ggcatcggcg
     8821 gtttaatgct agatatgccg acaaagaatc ctaaaacacg taagaaacca aaattcaaag
     8881 tcggtgacat ggttcgctgc gaagcagaag gattcatcta tccatttcgt ggatatgtag
     8941 aacacgtcta taatcactca gcaatcattc gcattgaaaa cacgatggaa tgtgataagt
     9001 ggttagcgaa aagcaaagag aatttagcag tggtgagatt ggtggacatg gaagttataa
     9061 aatcttaatt ttgctttttt acaactatcc tacaacaatg agcgttattc cacaactgga
     9121 aggagaagag gcaattgtga acattttaga gaatatagat attaagcaaa caaggaaaaa
     9181 tgctagacga ttactaaaaa gatacagaaa tttagaacgt ttggtcggac cagtgaaaat
     9241 agacttttcc gtgatgactg ttaccaaaaa tttgaaattc acaattgaca gtcaaaacga
     9301 agaaatcatt gaagcgataa gtactcgaga ttcggttatc gaagcattaa cacggctaag
     9361 tagaatccat ttccaggtac tttattatag ttattgtttt cctaataaga tgtcaatgta
     9421 tcaaatagga gaaaaattag gatattccga tagaactatc gagagaatga aggcagtagc
     9481 tttggttgaa tttgctgagg cgtataaatc aggagaactc atttcacgta caaaataaaa
     9541 aaagtcggat tcctccgact atgaataata tttccgacat aagtattata tcataattgg
     9601 gggaatcaga ggatggtact tttcgatgta aagaaatatg agacaccaag cgcgaaagat
     9661 gttgatatgg aacgcacaaa acataatgtc gctgtgtttc tttcagcata tctatcagct
     9721 agatgtagag tagggcaaca tcgagaacca aaagtgacag catcttattc cttggttcca
     9781 ccttctacag ctgatcatat atttgaagca gaaagaatgc tgatcgataa agaagaagca
     9841 caggaagaat ttgagtatct gcataaattg tttgttcgag gctattcagc gatacagcat
     9901 ccgcataagc ctgatgtgac tgaaaggcgc aagaagatat tctatgatcg ttatatcaat
     9961 ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc ggttaaagta
    10021 gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc tttcaagtag
    10081 ccagttttta cactttttat acccttttat taccaatttg gtttccattt tatacctttt
    10141 ttataccaat cacttaccta ttcaacgttg tattatgata gtgtcgaaaa attaggaaac
    10201 aggatcgaca aaataaaatg taagggagga aatctccctc atcgtttaat taagcttcga
    10261 taggcagcaa cggaaatatt aagaataagg atgtgaattt caactccttc taaattgttc
    10321 ttattatcta tcatccgttg ctgtctattg tttttttaat tattcacacg ataactaaag
    10381 gtaagtgaag agaaatgatt ccattaataa tttcaatttt tgcgctttgt cttaatgtat
    10441 atatgattgg atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat
    10501 agtgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca
    10561 accatgcaag gttcgattcc ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa
    10621 aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacataa
    10681 ctggttaggt tagattgaga tttgggattc ggtacaaatg aatcgtcaaa tgtctcaagc
    10741 acaggatcgg aaacgtccct gcctgtgcat tacatattag atcactcttt gagtggtctt
    10801 tttctttaca taaaggaggc tacataatga gaaactactg gtatgtatcg ctaactaatg
    10861 aatatcctcg aaccattgat gattgttcag tgcgtgttgt gcgttctgta caaatcaaag
    10921 ggaagtactc tattgtcgaa atggggagag aagcagaacc atgtgagatt gatgcgtgca
    10981 agctcgttta ttgcggtcat ggtttctatg atgaaccaaa cattcaaaat aatattaaca
    11041 agaatttgag ggattagaat gcaagaaatg gcttaaaaat ctccaatcag gacaaatcaa
    11101 gttagacaaa gtttccgatt taaagatatt gattgaagct gatttaatgc taaaagatat
    11161 tgaaaattag aaaacaaaac tcaacctaaa cagaatgtga ggtggtgtgt attgaatggc
    11221 aagacaacgt gatccaagac gtgatgaagc caaaagaatt tggttagaat ccaacggaga
    11281 aaagcagtta aaggaaattg catctgaatt aaatgtttca gattctcagg ttagaaaatg
    11341 gaaatcgcaa gacaaatgga gcgctgaatt gattagtaac gttaccaatg gcaaaagtaa
    11401 cgttactaat caaggtggcg ctcctattgg taatcaaaat gctaaaggta acaaaggaaa
    11461 tagccgagct tctccgccag tgggtaataa aaacgctttg aaaacaggtg agtatgaaac
    11521 aatatttttt gacacgttaa ccgataatga gaaggacatc tattctagtt tgaatgatga
    11581 tccttctttt gctttgtccg aagaaatacg acttcttaaa ataagacaac ttcgaatgat
    11641 gaaaagaatc caacaagctg aagctggact aaatgatgaa gaagtcgaac gattgcagca
    11701 gctaagaaag attaaaactc cgattgaaaa aaatggtaaa aagctagaaa tcaagcgtga
    11761 ggttatgcaa gatgtgcaga ttagcagaaa aaaacatcgc aaaattgatg atattctttc
    11821 aattgaagat tcattgactc ggattagcaa ccagttagct aaagccatca agcaaatgaa
    11881 tgaactttat atgaatgaat acagaactga tttaattaaa gctcagactg ataagatcca
    11941 agctgagaca aatgaaattg gcggaaataa ttcaggtgaa gaaatagaag aatggaaaca
    12001 ggcagtttta aatgccgcaa acaaacgggc ggtgaaagaa aatgaataat gaatttattc
    12061 cttttgctga tattggttct gccattgatt attactatga taaaccagta gctttttgcc
    12121 aagatatttt gcatttgaat cctgatgaat ggcaagaaaa tgttttaaat gatttagctg
    12181 aattttcaaa ggtttctgtt cgttctggtc aaggagttgg aaaaacagca ttagaagcag
    12241 gagcaatact ttggttctta acgtgtcgac cctacgctaa agtaatagca acagctccga
    12301 caatgaagca actttacgat gtactttggg cagaggtagc taaatggtta aatgatagct
    12361 tgatcaaaaa cttactgaag tggacaaaga ccaaaattta tatggttggt gattcagagc
    12421 gttggtttgc tacggctaga acagcgacta aaccagaaag tatgcaaggt tttcacgagg
    12481 accatatgtt gattgtggta gatgaagctt ctggtgtgtc tgatccaatt atggaagcta
    12541 ttcttggtac gctatcaggt tttgataata agctgttgat gtgtggaaac cccaataata
    12601 ttgaaggtgt cttttatgat tcccataatt cagaccgtga taaatacaga gttcataaag
    12661 tatcaagcta tgatagtaaa cgtacaaaca aagacaatat agaaatgatt cttaaaaaat
    12721 atggaaaaga aagtgatgtt gctcgtgtcc gtatttttgg agaatttccg aaaggtgcgt
    12781 tggattcatt tatcagtctt gaaacagttg aattggctac agaaaaacaa attagtgatt
    12841 ctttagtcaa taaaacaaag gttgctcata ttggtgtcga cgtagctcga tatggtgatg
    12901 attctacgat tctctttcct agaattgcta ccagggcatt ggagtatgag aagtattcaa
    12961 aacgtagcac catggaaaca acaggatatg tcatcaacat ggccaagaat ctaatgagtc
    13021 aatatccgag tattgataaa gtgatgatta aagtcgatga cactggtgtc ggaggtggtg
    13081 taaccgaccg cctagaagaa cttatagaag acaaacatta tccttttgag gtgtttggag
    13141 tgaataacgg ttcaacatca gaagacgatt tttacgataa tttaggtact caactatggg
    13201 gaaacatcaa ggaaatgtta gaagaaaata tgacagcaaa tcttaacgga gaacagcctg
    13261 ttattgaatt gccttctgat agttcgttaa tcaaagaatt aagtactcgc aaattcaaaa
    13321 tgacaagtag aagtcgtata cgtttagaaa gtaaagatga tatgaaaaag cgaaatattg
    13381 gtagtcccga tattgctgac gcactggctt tagcgtttta tgagccacca agtcactatc
    13441 aatttattca attttaggag gtgagctttt gattagtaca gtattatctt tagagagata
    13501 taagaagcta cgtgttaaat atgcaacaca aattgaagat ggaatgtttg atccgaatgg
    13561 ttttatagaa gatatgaaac cattttttgc tgatcgcgaa agaaaatatc tagcttatac
    13621 tagcgaaaag aatgaaatag ataatagacc aaagcctaac actgatattg taaaagttaa
    13681 taataaactt catgctggta tgtattcaat tgtcgtagac caagcagtca atcattttac
    13741 tggtatacct atcaaatggg attacgatgt atcggaacaa aaaagaacac tcattcaaag
    13801 attaaaagat aaattcttaa aaaatgatat agaacttccg acagttccag aagcttttaa
    13861 caaattaaca agcaatcttg attccatgag attcgcaatg cttgattctg aaactgcaac
    13921 ttttcaagga gcttgtggag tagctttccg attgctagag cctgtggaag aagatgatgg
    13981 ttggaagtta agagcaagta atattgaacc ttggagagca gaaagatatg gaaatgccgg
    14041 aatctatatc aaagaaaagt atgactctta ccagaaaaaa ttttttcaag aaatgaaagt
    14101 tatgacaaga aataaaattc tgacatatgc ttgctatggt gatttgaatt ttgttacaag
    14161 cggaacgttc aaaaaaattg atgagaccga taaccctttg gggacgatta ccttggcaga
    14221 atttaaaaat aatacgaatc gttattgtga ttttgaagta gctgaggaaa ttggagatgc
    14281 aattgatcgt gctttgtctg atcaacaaaa cgaaatcgaa caatttaagc ttgcgtacat
    14341 gcttgttact ggcacaacaa tgagcaaagg cactgctaaa gaaatgatga atcagctagg
    14401 gattatcaac ttaaaagccc ctactgcaaa agctgaatat gtcacaaaaa atttagcaaa
    14461 agatttcaat gaatatcata tggatctatt gaaaaaacag ttttacacca tttgtaaagc
    14521 aatcgatttt aacgatgagg tgtttaaatc aaacagttct ggagaagctc gcaagtggca
    14581 aatcattagc ctagaagcga aaacaaatac taaagagcaa tattttagag aaggattgaa
    14641 agaatgcgca gaaacaattg ctgcttttct taagtttcat gacaaagtag aaattgagcc
    14701 tgaaaaaatt atttttactt tctctagatc tttgccaact gatttaagtt atttagctga
    14761 agctttacct aaacttgcac catacgtatc caaacgaact attcagagcc aaattccatt
    14821 tgtaacagat gttgattatg aaaatgaaat gatggaattg gaaagtggaa gtgcttatcc
    14881 agatagtgaa tacaattttg gcggaggtgg caatagtgac gatagaaacg aagtattgga
    14941 ccaaacgtcg agaactggag gatcaagcaa ggctcaaaca agaaaatcag acacttaaaa
    15001 aattaactag tgtatttcct gaagcactga aagagataca agcaaaacta ttatcacaag
    15061 ctgacttaca caatatcact tatccagaaa tgatggagtt ttacagtaca agtaatcaga
    15121 aaaaatatcg tgaatatgtg gaaaaaaatt ataagtcatt aaaaatgtat gatgcaaaat
    15181 acaaagagtt tatcgatgaa ttttttccac catttgacta tgcaaaagtc aatcgcttat
    15241 tacaaatacg atcagatgta tttaaaattc ttgcagaata tgcgatggat gcagatgtga
    15301 atcaatattt ttctgatcgc ttagaggaaa ttcttcaaag aacatattct tctaatgcta
    15361 atgtttttgt tcaactttta aacgttgata taccaaatta tttaccagaa aatgaaatta
    15421 aacattattt gaactatcca tggtcaggaa agacattttc aagaagactt tggggaaata
    15481 tttcatcgct tgaacagaaa ctctctaatg ctattgtaaa aagtgttgct agtggagaag
    15541 gcgttataca cgcattaaac accatgagat tagactcaga aatttgtgac atgtttaagt
    15601 tagaagaatc aaagtataac aaagcgatag aaaatctcgt tcgaacggag tatgcaaaat
    15661 ttgcacaaga tggtattgaa aaatcatatt tagaaacagg tattgaggaa tacaacgtat
    15721 tgactgcaaa agatgagaga gtttgccgga tttgtggagg aaaggcaagt aagaatccct
    15781 ataaactgaa agatgctgtc ataggtgaaa atcgagcacc tttccatagt cgttgtagat
    15841 gtacggatgt tcctaattta ccaaaattag gaaaggatat tgatgaagaa tatgaccgtt
    15901 tatttggcga tttattagat gagtttgcac atgattcttt tggaattaat ttgaaacgga
    15961 ggaagtagaa tgaaagattt ttttgaagca gtactaacaa ttaatgtaaa tgctgatatt
    16021 gcagaagcct acaaaacagc tattgagtct gagaaccatc ctaatggctt gagagaccat
    16081 tggaatggca attatgccta cgtggttatt ggcgatcaaa ctgttaatta tcaagataat
    16141 actccagttg ataagaatac cgttaattta acgattcaat tattatctca ttcattacca
    16201 aatttaaaag aaacagttga ttggtatgaa aagatgggat gtattgttgt tagaactgac
    16261 tacaaagaag gaaagtctag taattaggct tttttatttt gtccgaaatg acgttaaact
    16321 agcgcaatac tgggcttggt tgaatggtgg ggcgcaacta ttaaaactca aagcaatgcg
    16381 gggcgtgaaa acgaatcgtg gggcgaaagg agaatgagca tgaaacacaa atcattaatg
    16441 ccaatgaatt tgcaatactt tgccgaaggt gacgatcaaa agttttcttt tgatgacttc
    16501 aaatcttttg tggaatccaa tgaagaagct caaaaattta ttcagtcaca atctcaaagt
    16561 gttgccgaca aacaattgga agcttggaaa caaaataact tagataaaat taaacaggat
    16621 accatcaagg aatatgagga atctaagaaa aacaagtcac ctgagcaaat tcaactggaa
    16681 aaactacaag ctgaatttga agcagaaaaa gcgttgcgtg tgacaagtga taataaagca
    16741 tttgttgcag aacaaattgc tggattagaa ctagatggag agttaaaaga gtctatttct
    16801 caatttatgc taaataatct tgttagttcg gatacagatt tcactaagaa tgctgttgaa
    16861 ggttttacaa gtgttttgaa tgcaattaat gagaaacatg cagatgcatt aaaagaacta
    16921 caaatgaagt ctgcatttgg tggaactcaa caatcgaata accaggttca gcagaacaat
    16981 gaaacattaa caaatccaga agaacaatta ggacaaattc ttcaacaatt taactaggag
    17041 agtgaaaaat tatgaaaaaa acatctttaa ataatttaga gtatttggat atttcaccag
    17101 cgattaatgc tatgcaagta ccaaatacac cttttttaag ctatttactt ggtgctggaa
    17161 aaacagagcc agcaaactcg acagaaatta aatggcgtga atatgatatc aacaacgatg
    17221 attcttctga aaaacttgag ggcggagaat atccagatgc tgaatcaggt cgaacttggt
    17281 ttaacaacta tactgaaatt tttagaaaat caacctctgt atctggtaca ttagatgcta
    17341 ttaatgtgaa tggagtcgga aatgaattaa ctaatcaagt agcactacgt ggtatggaaa
    17401 tgaaaattga tttgaaccga aaattgatta ctggtgtaaa agctgatgaa aatggttcta
    17461 aaggtcgtcg aatgaatggg attttgaact tgatcaattc agcaaataag gcagaaacag
    17521 ccactgcggg tgcagtaaca agaaaagata tcgatacttt atttaaattg atgtatgaaa
    17581 aaggttatat gggagaaaaa ctatgcttga tttctccaga tatgcaggag ttaatgactg
    17641 atgagttaga tggaaaatca acaaaaattg ttcagttcgg ggaaagagta acttttggat
    17701 tgcaaattgg aaatatcgtg tctaattacg gtacaggtat tgctctacta gaaccatcat
    17761 tgccaaaagg aacaattgcc gcaatcgata ctaattatgt gaaactacgt ccattacgtg
    17821 aatggagagc agaagaactt gcaaaaacaa cggattctag acgtatcggt cttgtaggtg
    17881 aatactctct tgaatacaat gcttcaaact ctggggcaat tttaaattta aagtctgaat
    17941 aaaagggagt tagtactccc ttttttgata ggaggaatta cagtggtaaa aaaagatgaa
    18001 actaaaaaag atgaagtcgt gaaatataga gtaggtaaaa ctaaaaattt tgttgggttt
    18061 gttcatccta aaactcgtag atttatcaca gcagattcaa ataacgaatt tatcatttct
    18121 atagatgata aagaagcaat tgcaattttg gaagatgcaa ttgatgttaa tgaaatttag
    18181 gaagtgatct gatggatgaa tcgctaaaaa cggaaatcat tgagtctaca aaagaagatt
    18241 ttccagattt gagtgaagaa cgcataacta atttattaga aataattttg ctagaaattg
    18301 aatcatacaa cacttgtaaa aatgatattt catgggaaaa gttaaaaagc gtgattagtg
    18361 aagtgttgta taaaataata aaaaatgaat cagaaaaaac agtatcttca gttagacgtg
    18421 gcgatacaac gattagttat gcttcaacaa caaatgatgt tagtgagtta cttctaggct
    18481 atggtgattt gatacgaaga gttattggtt gtggaggatt ggagtttttt taatgaatga
    18541 agcagatatt ttagaaacta cttatgaaga tagttgtatt attgaaagac tgacggacat
    18601 tgaagattct aatacaaata ttactattca agattacaaa aaagtatatg ataatcctat
    18661 ttcttgcgct ctttcacaag gtcaaatcga tggactagca gtcatagaag atggagagat
    18721 ggtaaatgtt tcaactgaca catataaatt atttgttcat cctaagatta aactaaaaaa
    18781 aggagatcga ataacaataa ctcaaaaggc cagtggctta attttttctc tatttgctac
    18841 taagcctttt tactatccta gtcattgtga agtgaatttg ataggaagtg agaaaaatgg
    18901 gtaatctcaa atatgaatca aatgcagaaa agatcattga gaattttaaa aatatgactg
    18961 taattgctca aaaagaagga atatccttcg ttaacgattc aatgaataaa gtcgttagtt
    19021 tagctaaacc attaactcct gtaaaatcag gtaatttaag gcgaggatat cgtgtagtaa
    19081 aagctagaaa actatcaagc ggtcgaattg ttggagaagt tataaacaat gaacattatt
    19141 ttaaatatgt agaagaaggt cataggacta aaaatggtgg ctttgtcaaa gggaaattca
    19201 tgttaactcg tgcaacgaat cttgcaaata tgtcttatat tcctcgaaga tttaaacaaa
    19261 tggcaataaa aatcgttaag aaaggaaagt aacatgaaag atgaaatcat tgctgcaatc
    19321 agtagcaaat taaaagaaat ctatcctgat gggacaatat atcttgattc agttatgcag
    19381 tcaactaaag atttttattt cgttttatca gtaatggaat ctggaactga aaatgtagga
    19441 attgatattc aaaatgtttc tttcttaatt gatattgcat tgattgataa taaccctaat
    19501 agaaatttaa ttaatgaatt agtctcacgt tgtgggactt tttttaatac gattacaatc
    19561 gatgagcaaa tattatttcc aaaagcctat ttacctgatg aagcagatgg tgttcaacat
    19621 attcgtttta cattagaatt tccacaatat attgaatgga gtgaaagata aatgggagaa
    19681 aaaagaagta aaaccggaat tatttctgtc gaaaaaccta cttggtttcc tttggaagat
    19741 gagacaggga attttccggt atatggtact gcgttgccaa tgggaacagc agtaagtatc
    19801 aaaccaacag ctaattatga aacaacacaa gattatgggg attcagtggt tcaagatcaa
    19861 tttacagctt ttggtggtgc agaagttgaa ctggaagcaa atggatatac tcataaagtt
    19921 ttatctacaa ttactggagg gaaaattgtt aaaggtggtg ccttgcgttc tggagaagat
    19981 attgcgcaag atggtgcgtt tgcatataga cgtaaaaaat cgaatgggaa ataccgttat
    20041 acggtctttt ataaaggaca gtttgctcta gattcagatg aaacatcaac gattgaagga
    20101 agtaaagtaa gttttactca tccaacttgg aagggttcat ttgtagatgt accaggactt
    20161 ggatatatgt attcagttga tgaagatgat gaaggcgtgg ataaagcaat gatcggaaat
    20221 tggtttacaa aagtagcaat tccaattgaa gaagcagaac tttcaggagg tacagaataa
    20281 tgtcaaaata tcaaacaacg attaaattaa cgaaaaaaga cgaagaaggt aaatatgagc
    20341 aagtacaatt taaatctgct gaatttttac caggaacagt tgtagaagat gctgcaggag
    20401 ttatggaaga aatgcaaaca gcgactgata agcaatcagt taaaaaagct ttaagtcgtg
    20461 cttattcatt tattgcagat actttatttg agggacaatt tactggtgaa gattattgca
    20521 aaggaattga tgctcgtgag attgcctcat tgacaggaaa attattgaag tctgttactg
    20581 caggttttga tgaaacttat acagagacga aaaaaaagta agtgaggcgc tcaagtcacc
    20641 ttcatttaag tattcgatta cttaccgaga attagatata aagacgcaat tactcgaagc
    20701 aggttggacg ttaccagaaa ttgaacgtac tgacttggat gagttaatgc gtctttttgc
    20761 ttttagagat gcggtaaaag agcatgaaga tgttgagtac tatgataatt tcactcaatt
    20821 ttaggaggtg atactttgaa taacgatgac ctaattctga agatgatact agatgaatct
    20881 ggttttactg ccggtatgaa taatgctgtc aaaaagttga actcttttga tgaaacaatc
    20941 gaaagaaaaa gtagaaatag tggcagttca ttaggtagta tttggaaaat atttgctgga
    21001 agttttctag ctagcggagt aactagaatc gtaggagctg gctttgatct aatcaagggt
    21061 tccataagtg gagcggttga tcgagtagat acgatgaata acgctctacg aaatttccaa
    21121 aatatgggat ttagtaattc agaaattatg aagaatatcg gaaagaatgg gcttttatct
    21181 caaggtattc aaggacttcc tactgctttg aatgatgcga taagtcatgt tcaacttctt
    21241 gcttcttcta caggtgattt aactcgttca actcaaatat ttaaagcttt aaatgatgga
    21301 attcttggtt tcggtggttc aactgatcaa gttaatgaag ctgttattca attatctcaa
    21361 agtttctcaa acggaaaagt agacgcacaa acttggaact caatgatcaa cgctcaactt
    21421 ggacctactc tttctgctat tgctaaaaag atgggaatta caatgggaga tctgaaagaa
    21481 ggtttatctc aaggtaagat ttctgttgaa gaattccaaa atcaattaat agaaatggat
    21541 accaaaggtg gcggaggact taaatcatta agtcaaatcg ctaaagattc tactaaggga
    21601 attaaaacct ctatacaaaa tgcaaaaaca gctgttacac gtggtgttgg tgaggtaata
    21661 gaaggattaa ataaagcatt agtagattca gatttaggtg gatttaaggg aattattgat
    21721 aaagtgggca gttcaatgga atcgttctta aaagtaattg ctgcaaatat tcctacagca
    21781 gtatcatttt taagtaattt atttaacgaa attcaaaaat ttggttctgc tttgaaattc
    21841 atgatgccct ttcttgttcc tgcagctacc gcttttggag cgttcatgtt tcaacttaaa
    21901 ggtataccag caataataaa aagcttcaat aattttaaga atgccataat tggcgttgga
    21961 aattcattaa agattatggg ggcaatagct gctgctaatc catttgtttt aattattggt
    22021 gcagtcgttg gtgcaatagc tgttttcggt tactttatgg caaccaatga agagcttaga
    22081 aacaaggtta tatctatttg gaatgatgtg aaagattccg taattggtgt attaaaaaat
    22141 ataaaggact ggggaattga tacttggaat tctgctaaag aaatggcatc aaatgcagtt
    22201 gagggtgtca aagatgcttg gtcaggaaca aaggaatggt tttctaacac ctggaaaggt
    22261 attaaggatg gggccacagg tttatttgat aaaacagtag agacttcaag aaatgcagtc
    22321 gatagtgtaa aaaatgcatg gtctaatatg aagaaatggt tttctgatac ttggcaaagt
    22381 ataaaagatt cagccagaga aaaatggaat ggaatcaaag gttctattat gtcagttgct
    22441 ggtccattga ttacaggtat aaagaatgca tttttacatg ttacttttta tctagagacc
    22501 ttatggaata atttagttga gattggtaaa aacgtctttg agattttaaa aaatgttata
    22561 cttgcgccgg ttttgtttat tacctccatg atttctggtg gatgggaaga aacaaaaaat
    22621 aatatgattg ctgtttggaa taatattaaa gaaagtgcca taaatatttg ggaatctata
    22681 aagaatatat ttgtaagtta ttttacaaat atttattttg ctgcactcaa tatttgg
//
LOCUS       DAZKCE010000043        22419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000043 DAZKCE010000000
VERSION     DAZKCE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.043"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8KQ_001457"
     CDS             6..305
                     /locus_tag="ACG8KQ_001457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9104397.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8KQ_001458"
     CDS             378..1739
                     /locus_tag="ACG8KQ_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9104398.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8KQ_001459"
     CDS             1761..2021
                     /locus_tag="ACG8KQ_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104399.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8KQ_001460"
     CDS             2035..2787
                     /locus_tag="ACG8KQ_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9104400.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8KQ_001461"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8KQ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9104401.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8KQ_001462"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8KQ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9104402.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8KQ_001463"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8KQ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9104403.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8KQ_001464"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8KQ_001464"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9104404.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8KQ_001465"
     CDS             7161..8558
                     /locus_tag="ACG8KQ_001465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9104405.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8KQ_001466"
     CDS             8639..9079
                     /locus_tag="ACG8KQ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9104406.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8KQ_001467"
     CDS             9227..9553
                     /locus_tag="ACG8KQ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9104407.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8KQ_001468"
     CDS             9554..10303
                     /locus_tag="ACG8KQ_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9104408.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8KQ_001469"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8KQ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9104409.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8KQ_001470"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8KQ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104410.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8KQ_001471"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8KQ_001471"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9104411.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8KQ_001472"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8KQ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9104412.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8KQ_001473"
     CDS             14770..17436
                     /locus_tag="ACG8KQ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9104413.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8KQ_001474"
     CDS             17800..18552
                     /locus_tag="ACG8KQ_001474"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9104414.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8KQ_001475"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8KQ_001475"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9104415.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG8KQ_001476"
     CDS             19913..22354
                     /locus_tag="ACG8KQ_001476"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9104416.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3932 c         4672 g         6617 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttttt
//
LOCUS       DAZKCE010000044        22257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000044 DAZKCE010000000
VERSION     DAZKCE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.044"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            327..2162
                     /gene="tdc"
                     /locus_tag="ACG8KQ_001477"
     CDS             327..2162
                     /gene="tdc"
                     /locus_tag="ACG8KQ_001477"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9104417.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2467..3945
                     /locus_tag="ACG8KQ_001478"
     CDS             2467..3945
                     /locus_tag="ACG8KQ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9104418.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3911..5242
                     /locus_tag="ACG8KQ_001479"
     CDS             3911..5242
                     /locus_tag="ACG8KQ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9104419.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5356..7908
                     /locus_tag="ACG8KQ_001480"
     CDS             5356..7908
                     /locus_tag="ACG8KQ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP9104420.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7915..9354)
                     /locus_tag="ACG8KQ_001481"
     CDS             complement(7915..9354)
                     /locus_tag="ACG8KQ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104421.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9329..9511
                     /locus_tag="ACG8KQ_001482"
     CDS             9329..9511
                     /locus_tag="ACG8KQ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104422.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9700..10062
                     /locus_tag="ACG8KQ_001483"
     CDS             9700..10062
                     /locus_tag="ACG8KQ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9104423.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10249..11106)
                     /locus_tag="ACG8KQ_001484"
     CDS             complement(10249..11106)
                     /locus_tag="ACG8KQ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9104424.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11226..12137
                     /locus_tag="ACG8KQ_001485"
     CDS             11226..12137
                     /locus_tag="ACG8KQ_001485"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9104425.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12144..13790
                     /gene="mdcA"
                     /locus_tag="ACG8KQ_001486"
     CDS             12144..13790
                     /gene="mdcA"
                     /locus_tag="ACG8KQ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9104426.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13892..14191
                     /locus_tag="ACG8KQ_001487"
     CDS             13892..14191
                     /locus_tag="ACG8KQ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9104427.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14181..15857
                     /gene="mdcD"
                     /locus_tag="ACG8KQ_001488"
     CDS             14181..15857
                     /gene="mdcD"
                     /locus_tag="ACG8KQ_001488"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9104428.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15861..16454
                     /locus_tag="ACG8KQ_001489"
     CDS             15861..16454
                     /locus_tag="ACG8KQ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9104429.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16478..17554
                     /locus_tag="ACG8KQ_001490"
     CDS             16478..17554
                     /locus_tag="ACG8KQ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9104430.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17579..18448
                     /gene="mdcB"
                     /locus_tag="ACG8KQ_001491"
     CDS             17579..18448
                     /gene="mdcB"
                     /locus_tag="ACG8KQ_001491"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP9104431.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18559..19323
                     /locus_tag="ACG8KQ_001492"
     CDS             18559..19323
                     /locus_tag="ACG8KQ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP9104432.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19812..21905
                     /locus_tag="ACG8KQ_001493"
     CDS             19812..21905
                     /locus_tag="ACG8KQ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9104433.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21930..22232
                     /locus_tag="ACG8KQ_001494"
     CDS             21930..22232
                     /locus_tag="ACG8KQ_001494"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9104434.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7219 a         3792 c         4733 g         6513 t
ORIGIN      
        1 ataattctat taaaaaataa attgcgaatt tttaacaata taaaaaaata aaaatatgtt
       61 tagtgaatga aggacagtca ttttatttgt ttgttcgctt tttttaggtt atctatttta
      121 gaagactgat ttattgaaaa attgttcgct tttttgcgtt gaattatcag aaaaatccga
      181 ctttatttat ttttgtttgt aaaaatctac gtggatttga tgttataaaa aaaaaattct
      241 catttttggc gctatagtga agaggtgtta cagataaaca catgtatctg caagacattg
      301 accaacaaaa tatggaggga agaaaaatga aagatatgga tatcaaggcc gtctttatag
      361 gcgacaaagc tgaaaatgga ccagtttata aaatgttgtt gaacaagatg gtagatgaac
      421 atcttggatg gagagaaaat tatattcctt cagatatgcc tgcaattagc gaaggagata
      481 agctcactcc agattatcta gccacccgcg accatatgat agaggtcttg gatgaagtca
      541 gtcagcgatt acgcgcagga tcgatcccat ggcattcagc aggccgttac tggggacaaa
      601 tgaatgctga aacattgatg cctgctttat tagcttacaa ctatgcgatg ctgtggaacc
      661 caaataatgt tgcattggaa tcttctatgg caacttcgca aatggaagca gaagtaggac
      721 aggattttgc ttcattattc aatatgacag acggatgggg acacattgca gcagatggtt
      781 ctattgctaa tcttgaagga ttatggtacg cccgttgcat caaatcgatt cctttagctg
      841 tcaaggaagt gctgcctgaa aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat
      901 ctgttgaaga aattttggaa atgaccgaaa gctttacgga cgaagaaatg gatgaggtca
      961 aagcagcttc ttcccgtagc ggaaaaaata tccaaagatt agggaaatgg ctggtccctc
     1021 aaacaaaaca ctattcttgg atgaaggcgt tagatatttg cggagtcggc ttagatcaga
     1081 tggttgctat cccagttcaa gaggattatc gaatggatat taatgctttg gaaaaaacaa
     1141 ttcgtgaatt agctggacaa aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga
     1201 cagaagaagg tcaagtagac agtgtagata aaatcgtcca attaagagaa aggttaaaag
     1261 atgaagggat ctatttctat ttacatgttg atgccgcata tggcggctat gcgcgttcac
     1321 tgtttctaaa cgaagcagga gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag
     1381 aacatcatgt tttccaccac tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag
     1441 ctatttcaga agcggattct gtcacgatag atcctcataa gatgggctat gtcccttatg
     1501 ctgctggagg aatcgtaatc aaacataaaa atatgagaaa tatcatttct tatttcgcgc
     1561 catatgtgtt tgaaaaatca gtaaaggctc cagatatgtt aggagcttat attttggaag
     1621 gatcaaaagc tggagcaacc gccgccgcag tctggacagc acatcgtgta ttgccattga
     1681 atgtgaccgg atatggtcaa ttgattgggg cttcaatcga ggcagctcaa agattcagag
     1741 aatttctcga tcatctgact tttactgtta aaggaaaaac aatcgaagtc tatccattga
     1801 atcatcctga ttttaatatg gtcaattggg tattcaaaga acaaggctgt acagatttga
     1861 acgctatcaa tgaattaaat gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg
     1921 tttatggtga acggtttatt acttctcata ctacatttac acaagaagat tacggtgatt
     1981 ctccgattcg ttttgttgaa agaatgggtc taacgaaaga agaatggaaa aaagaacaga
     2041 aaatcactct gctacgtgcg gctatcatga ctccttattt gaatgatgat cgaattttca
     2101 atttttatac aaaaaaaatc gccaaagcaa tggaaaagaa actaaacgaa atcatccaat
     2161 agaactgatt tttgtgtgaa agaataacaa gagacatatg gagaacagac aattatttgc
     2221 ttcatcaaaa caattgtatt tccccctgtt tttcatatat tcctcgccaa gatattcttt
     2281 tttataaaac gttctataag ttggatcgtt cgtttcatgt atcaaaccca gttcactaag
     2341 atcgttcaat ggatgtttcg gtgatactgg gttttgtact gaatcgatga aaccttgtca
     2401 tttctgttct tgaacaaaaa taaaagaaaa caaaaacata tgtcgctatt gaaagaagga
     2461 ataaagatga aagatactgg ttcatcaaag caaatcacga ccatgacctt tatcggaatg
     2521 acctgcgccc ttgtagcaag tgtacgaaat attccagatg tagcagcaac gagctggacg
     2581 atgatttttt acatgctggt tgcagtatta ttgtacgctt ttccaatctc attgatctct
     2641 ggtgaatttg ctggaatgtt tccccaaaaa ggtggtccag aactttgggt atcgaatgct
     2701 ctaggaaaaa aatgggggtt cgttgtctca tggctgttat gggtccaaat gtttcctggg
     2761 atggtgatgg tcgcttcagc actcgctccg ttgtttggta atattatcga taatgtgccg
     2821 cttggtttga acagcaaatt tacattagtt gtcattttag tcgtttattg gatcatcact
     2881 tttttgaatt tgaaatttga tatggcaaaa atcggcggaa aagtcggtgt ttggttagga
     2941 ttgtacgtgc ctttgacgat gatgctctta ttagggtttg ctgcgtggat caaagttggg
     3001 atcgtaccaa ccggaacatt aggcagtttt tcatgggaca aattaatacc agatactacg
     3061 actgcatcct catttgttta ttttgcgccg atcatgttta tttttactgg tatcgaaatg
     3121 tcctctgtgt atatcactcg attggaaaat ccagttaaaa cgtatattcg cggagtattt
     3181 gccgcgttaa tttttatatt tttcgtcaat attttgaatg ctttagttgt agcaaatgtc
     3241 gtaccaaaag gacaaatgga attgaataat atcgcgcaat ctatttcgat ttattgccaa
     3301 attcttggtt tgccacatct tattgtgaat ctgtttagtt tacttgtctt cattggtgta
     3361 gcagtccaat tatctgcttg ggcctcagga ccagcaaaga cagtgacaga aagtgctaga
     3421 aaaggtgcgt atccaccaaa attcaatttt tggaagacga atcaatttga cgtatccaaa
     3481 tccgttattt tgactcagtc tgtcattatc tcaatctttg cactttttta tttgctgatt
     3541 cctggcgtca atcaagcatt tttgatgctc gtaaattcaa ctactgttat ctactgtatc
     3601 gtctatgtca tcatggggat cgcagttttg agattgcgtt atacacatgc aaagttgaat
     3661 cgaccattta gaattggaaa aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt
     3721 attgttttat ttgcggcaat cggattttct gtcggactga cgatgaaagc tggaacatgg
     3781 atcaatctaa ttgcagtcac agccatcagt gtcattttgt ttgtagtacc actctgtatt
     3841 gaaaaaataa agaaaccttc ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa
     3901 ggtgaacttg atggaaaaac cggcaatgaa acggactcta actaatcgaa atatccagtt
     3961 aatcgcatta ggtggcgcaa ttggaacagg attattttta ggttcagcaa aggcaattgc
     4021 atcagcaggg ccagctgtta ttctcgttta tttgatatgc ggtttggcta tttatggttt
     4081 gatgcgggcc atgggtgatt tatttttagc aaatagtaca tttaactcta tcagtgattt
     4141 cgttgactat tatttaggca aaaagtgggc attttttgtc aattggactt attggctttg
     4201 ctggcttgga gctggacttg cggaattaac agcaatagga ttgtatatcc atttctggtt
     4261 tgtacatgta ccagctttga taagcggagg tagcgcacta atgatcttaa tggcagtcaa
     4321 ccttgttgca gtcaagtggt ttggtgaatt ggaatctttt ttctcaatca tcaagatctt
     4381 ggggattttc ctttttatct tattgggtat aggattgagt gtctcagatt ttcataccca
     4441 gtctatcgga atcaaagaaa attttggaca gatcgatgaa ttactaccgt ttggctttgc
     4501 tggaatggta gtctcatttc ctatggtttt attcagtttt gctggaattg aaatgatcgg
     4561 cttaaccgtt ggcgagacaa gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct
     4621 gccttggcgt attctctttt tctatatagg aacgattttg tcgattttat ttgttgttcc
     4681 atggcattgt ttagatttaa aagaaagtcc tttagtgact atgctgcaag tgatccattg
     4741 ccaaggcgga gcagcaattg tcaatctaat gattttggtt gcttctttgt cctcagcaaa
     4801 tagtgcagtc tacagtacta gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc
     4861 ccattggttt tgtgcgcttt ctaagaaagg cgtaccttta cacggtattt taattacagg
     4921 aatgttattt tttgccggtc tttgtctaca tttttttgta gctgatagtc gattgttttt
     4981 tcagcttttg gcaggcatga caacgagttg ttttctattt gtgtggtctt cgatcttgtg
     5041 tgcacatgtt caatttatta aagaaaagaa acggaacact acacaaagat ataaagatct
     5101 tgctttgttg gtgtttttta cttcaattgc tttcagcttg ttattcgaaa gcacgacacg
     5161 ctttattcct tttttattgg ttatctggtt tgtattctta aattttgtat ataaaagaat
     5221 aagcaataga aatatcagtt aggcccagat acattttgca aaacgaatta tttcagaccc
     5281 ccctgaaata atcgatacgc ctgaaaaaac ttccctgcgt ataataacag tatcaattaa
     5341 acaggagggg ataggatgaa ctggtacaga ttgacggtag aagatgtatt gaaaaactat
     5401 caaacatcaa cagaaggctt gtcgcaagaa gaacgacaga aacgcctgac tgatcaagga
     5461 tataatgaaa taaaagcaag gcagcagaca aaacgatgga aaaaaatagc aaagcatttt
     5521 actgatctgc tgatgatcgt gttgatcatt gcctcttttt tgaaatttgc ttctagtgaa
     5581 tatattgaag gaagtattat tttattcgta gtgatcgtca atggactcgt tagttactgg
     5641 caagaaagaa aagcggaaga atcattgaat ggtttgaagc agctgatggg acaagaagcg
     5701 atagtactat caaacgggat gcaagaaaaa atccctgcga aagaactggt aaaaggagat
     5761 gtcattgtct tacgtgccgg agatgtcgtt ccagcagata tccgattaat cgaagcacat
     5821 gatttattag tggaagaatc gattttgacg ggagaatcag aagcatctga aaaaagtcat
     5881 gccgttttga cagaagaaga atcgatcggc gaccagaaaa atatgggttt ttcaggaacg
     5941 cttgtacaat ctggttctgc agtcggtgtc gttgtagaaa caggaaacac gacagaaatc
     6001 ggtaaaatca atcaagcatt gcaatcggtc gaacaacaaa cgacgcctct gattaaaaaa
     6061 atcaaccgat taaacaaaca aatttttcaa ggcattcttt gtttgagcct attcttagtt
     6121 atttttacga catttcgata tggtatggat tggcatatcc ttctttcggc tacgattgct
     6181 ttggtggtgt caatggtacc cgaaggattg ccagctgttt tgaccatgat tctatccgtt
     6241 ggagtacatg aaatggcaaa agaaaaggcg atcatcaaag gacttccttc ggtagaaaca
     6301 ttaggttcta tgacagtgat ctgttcagat aagacaggaa cattgacaaa aaatgaaatg
     6361 acagttgtgg atgtagctgc aaaagaagaa gcctgcgtgt tgtccattat gaaaaactgt
     6421 caggaattaa aaacaaaaga agaacagaaa acagaaaatt taagtggcaa tccgacagaa
     6481 gttgcgctga ttcgacacac agaaacagcc aatctacctt tgaaaaaaat agaagcaaag
     6541 attccgttca gttctgaata caaatatatg gcaacgatgc atgcggagga aaaaggagcg
     6601 gtgatttacg tcaaaggtgc accagaagta ttatttgcta aatcaactct ttcttctgca
     6661 gaacaagaag catggagtca gacagctgca gaatttgcga gaaaaggtca gcgtgtttta
     6721 ggatttgctt ataaaaaagt agattcgaaa caggaactca cgcatgagac tttaacccaa
     6781 ttaacttttg cagggatcgc cggattgatc gatccgccaa aagaaagtgc agtcaaagct
     6841 gtaaaggaat gccaacaagc agggatctca gtgaaaatga ttacagggga tcacaaagac
     6901 acagcaaaag cgattgcgga acaaattggc ttaaaacata ctgccaaagt tttagaaggg
     6961 atcgatttgg atcttatgtc tgatgaagag ttgattcagc aagtgccaat agtcgatgtc
     7021 tttgctcgaa cgacaccaga acataaactg cgaattgtta aagctttgca gaaaaatgga
     7081 gaaatcgtcg gaatgaccgg agacggcgta aatgatgcac ctgctttgaa aagatccgat
     7141 gtaggaattg ctatgggaat caaaggtagt gaagtaagca aacaggcagc tgatatggtt
     7201 ttaggagatg ataatttcca tacgattgca aaagccgtaa aagagggccg gcggattttg
     7261 gataatttgc aaaaaaccat caatttcttc ttgccaactg cattagctca aggattgatc
     7321 ttgatttggg cacttatgct gaatcgcccg ctacctttgt ccccagtaca gattttatgg
     7381 gtcaatatgg taacgacaat cactttatct tatgcactag ggttcgagtc gccagatcca
     7441 gaaatcatga agcgcccacc aagagatcca aagcaaggaa tcctatctgg ttatcatttg
     7501 tttcggatca tctatgtctc cctactgatc atggttcctg cctatttatt ggctatgcag
     7561 tttgaagggc aaagcttaca gcaaaccatt cttctgcaaa atatcgtctt agctcaagct
     7621 gtttacatga tcaattgcag agagttgtcc aagccgtcat tgaaccaagg tcttttgcaa
     7681 aacaaagcgc tttttctttc attaggtatc cttgctttat tgcaagggat ggttattttt
     7741 ctaccagtcg gccagcagct gatcggtaca gtaagtttaa cgatgacaca acagttattg
     7801 attggagcaa atgtgattct tctattctta gttgtagaaa tagaaaaagg gatcatgaac
     7861 aaactgtttc gcaaaaaaga aaaaacagct agagaataca gtcgctagcc gttactataa
     7921 aaaagttttg aaagtgagtt cagtcaaacg attgagctca tttttcgttg gaatagaaga
     7981 aatgtataca atagggatat ctagagaatc tagagggaaa ttggaaatca agatatctgt
     8041 tttttcaaaa gaaatagtgg aaagatcggt tagttcgata ggtaagaagc agacacgttt
     8101 tcctaagtaa tcttttaatt cctgctgcaa gaacgccttc catgaaggtt ctccttggaa
     8161 aataaaatag acattgaaca aattcggttt gatagaaaaa atcgtttgtt ggatcagtag
     8221 agacaagagt tggagaaagt aatttggttc attgatttct atttcttttt ttagatcgtt
     8281 cagtaaattc tctttctgtt gatacaagtg tgcatattgc tgatcatatg tttctaaaaa
     8341 acgatggtat tgacggtcca atgcttcaat caatatcggc gattcatgat attttaatat
     8401 gaggagagcc aagttatcag tcagagatgt tttttttagt tgatcaagag acagctctga
     8461 agctagttcc tctacaaacg tttcgaggac ctctaaaaat ggagaatgat gcaggcagcg
     8521 agcggtttct tgtccatagt tattgttgta attccaactt tccagaaagc agaaagaagc
     8581 aaaagaaagc tcagtgtctt taagataaat accttccgtt aaatacaatt ttttcaatgc
     8641 agagtcatac agggaggaga gtgtgctgtt ggtatggata gaagaatcta atcggcaatt
     8701 cgcttttata cggatcgtat tgataaagaa ccaacatgtt ccgaaaatct cttctgtttc
     8761 aaccgctagt tcagtttgac tgaggtttct taaaaattga aaataatggg attcatggat
     8821 cgaataatcc tcgaaaaaat agttgttatg agtgaaagga atcagcaatc gatgataaaa
     8881 agtacgaatc gcacgctctt ttccaagaat acaagctgta tatttcgtca aatcgatctg
     8941 caagtcgtaa cttgctaaac gctgattgat tttagtcaca tgtcttttca atgtcccata
     9001 agaaagccca tggtcggcta gaaaatcagg gacagcaact ttttttgtaa aaagcagatt
     9061 tttcagcagt tcgacactga ctgattgttt gaaaaaaatc tcccaaaggt catttgttaa
     9121 taaattttgc tggttttgca aactgattcc tgcttgatcg ctgatgatgt cccagccttc
     9181 gggcagctga gagcgaatag attgaaggtc agaaaaaatc gttcgttcgg ttgtccctat
     9241 gatttcagct aattcattta cggttatctt tgtatgattc agcaattgtt ctactaaaga
     9301 aacatgacgt cgaatatctt tttcggtaat gatttttttc attagtgaat acatcaatca
     9361 gactcccttc tattctaaga atacggagaa aataaacggt ggtcaagttt caactgattt
     9421 ttcaatcatt gtttattttt ctggagcatt tactgatgat aagaatttca tcatgaactt
     9481 gaaaaatgat accactaaaa tcaaaaaata ggtgggcaaa caataataaa cattttaata
     9541 gtagatggta gcaagaccaa cacaggcgga ttgaacattt aagagttgat aaaaaagaaa
     9601 aatatcgaag tatttgatta gcaactactt gtattcattt ttctattctt acgtggtatc
     9661 tttgaataaa gcgaatcaat aagaaagaga tggaagcaga tgctaacgaa aaaagaacta
     9721 atcgatcaca tcaatgaaac atacaatttg tctccagaat atatttttaa aaaatttcct
     9781 gattattgcg tattcagaca tcaaaaaagc aaaaaatggt ttggggtcct gatgtctgtt
     9841 ccagaagaaa aaatctatga tgatgcaagt gaccatagga tcatcaatat tcttgatttg
     9901 aaggtcgatc ctgaacttgg cggaattttg cgaggaaaag aggggtattt tccagcctat
     9961 cacatgaata aagaacattg gatctctatt gatctacgta aaatagaaaa gctagatgaa
    10021 ctagcagatt tgattgatgg aagttttaaa tcaacgcaat gaatgtctag gacaatagag
    10081 aagaggttgt gacagaaatg tttagcttca agaaataaga aggaatttac gaaaattgct
    10141 tctcaaattt ttgggaaatt tcggcttatt ttctaagaaa ttacttctgg aacaccgttt
    10201 gttcgaaaaa agagctgcga caagaatttt gtcccagctc tttttttttt attttcctaa
    10261 ataaaattcc cgcatcgttt ttgcaatcat tttgatcaaa ggatgatctt ctgtgaagct
    10321 agaagataaa taaattgttc gtgtcaaggg caaatctttg atttcccgca agacgaaagg
    10381 atcattttta tcgtaagacc aagaaacttt tgggaaaaag ctaacagcta atccttgctt
    10441 gacaatagaa cgtaatgtcg caggatcatc tgtcgtcgaa ctgatcgtca attcgatact
    10501 tttcgcttca agtgctgcat ccagtgtatg acggaaggcg ttgctctttg acagcatcaa
    10561 gatgggggtg cgttggatat cttctaattg cagatgttct tttttagcaa gaggatgttt
    10621 cttaggaatg gcgacataaa caggttcttt catgatagga atattggtca tgccaggctt
    10681 catctccgaa gtgatgtaca gtacaggatc ggtaatatca tctggtaaat gatgttgcat
    10741 gatcgttaat tggatatcag gatgcttttc cataaaaatt cggaccaagt tcggaataga
    10801 aacagaggct acctctaaat acaatatgac ggaagtaacg atcttctcat tgaccgaaga
    10861 caataatttg attccttctt ctaatgtgtc taatgtcttg actgcatatg gataaaattc
    10921 ttctgcataa acggtcgtct caatgtttct gccgctatgg taaaacaaag ggacacctaa
    10981 ttcgttttcc aaatgtttga tcgcacggct caaggcaggt tgagaaataa ataattcttt
    11041 ggctgcttgg gtaacatttt ttgtttcgaa tactttttta aaatactgca gatgaagcaa
    11101 tttcataatt atccatccca tccatacctt tttgttatgg ttttttcttt ttttggcatt
    11161 ttttattata gcagaaattg actaagatag cagtggaaaa agtaagtgct gaggaggcga
    11221 ggctcatgag tacaggtttc ttatttcctg gacaaggcag tcagaagtta gggatgttag
    11281 cggatatccc tgaaatgtac atggaacgtt tagaaaatgt gacggggtat cggttagttg
    11341 ataaagaaga aaactatcaa gatacggttt ttatccagct agcattactg acaaaagcag
    11401 tattttattt agatgaaatg gaaaaaaacg gtattcagcc tgatattgca gcaggacatt
    11461 cgattggtgc tttttgtgca gctgttgctt gtcgatcttt gagatttgag aatgcagcgg
    11521 aactagtcta tcaccgcgca gtcttaatga aagaagctta tccaagcggt tatgcaatgg
    11581 gcgtagtagt aggtgtgacg agaagcgaag ctgaggaaat tgtagcaaaa acatttgatc
    11641 ctgaccatcc agtttatctg tcaaatgaaa attgtccgat gcaacatacg ttatctggga
    11701 gtatcgaagg tttatccgtt acgttggcag aagccaaacg gcaccaagcg caaacagcga
    11761 agctgctgaa agtccctgtg ccatctcact gtctcttgat gaatgatacg gtcaagaaat
    11821 tcaagccgta tatgcagtct gtccatctat ctgatgctag atgtttgtat ataaaaaata
    11881 cggatggaag agccactcag gacgcagaag agattcgtaa agatttgttg gaaaacctag
    11941 ctttccctgt gcagtggaac caaatgatgg acttggcaaa agaattagga atggatctga
    12001 cgattgaatt tccccccggc aatactttga caaagctgat tcatacaaaa tttggtgtag
    12061 ataaagggat ccgtacgatc aatctagacc aacatggtat tgatgacgcc acgtttctat
    12121 atcaaaaatg gagatgacta attatggaaa aaccaaaccg caactgggct tctaaacgcg
    12181 aaaaaaaagc gcagcgattg gaaaaaatca gcaacttgct caatggcaag tatgtagaaa
    12241 ctgaaaaaat cgtagaagta atggaaaaac tgattgctcc cggcgacaaa gttgtattag
    12301 aaggcgacaa tcaaaaacaa gcaagttttc tttcagaaag tcttgaaaaa gtagatgcag
    12361 aaaaagtgaa tgatctgcat ttgatcatgt ctagtatctc acgaccagaa catttgaata
    12421 tttttgaaaa aggaatcgca agcaagatcg atttctctta tgctggtgca caaagcttgc
    12481 gtgtcgccca aatgattgaa gacggcacaa tgaaattagg tgatatccat acttatttgg
    12541 aactttatgg acgcttattc atcgatttga ttccaaatgt tgttttagta gctgcagata
    12601 aagcagataa agacggcaac ttgtacacag gtttcaatac ggaagaaaca ccaacaatca
    12661 tcgaagctgc agctttcaaa gatggtatcg tgatcgttca agttaacgaa gttgtcgatt
    12721 ctttaccacg tgtagatatc cctggtgact gggtagacgt tgtcgtgaaa gcagataaac
    12781 cttatcaatt agaagcttta ttcacacgag atcctcaaaa catcacagaa ttgcaaattc
    12841 taatggcaat gatggcaatc aaaggaatct acgcagaaca cggcgtacaa tctttgaatc
    12901 acggtattgg attcaataca gcagctatcg aattgttact gccaacttat ggtgaatcac
    12961 tagggttaaa agggaaaatc gcgaagaact gggtattgaa cccacatcca acgatgattc
    13021 cagccatcga atcaggttgg gttgaaagca tccatagttt tggtggcgaa gttggtatgg
    13081 aaaaatatat tgctgctcgc ccagatgtct tttttgtcgg taaagacggc acgatgcgca
    13141 gtaaccgaac attatcacaa gctgcaggac aatatgccat cgatatgttt atcggttcaa
    13201 ccttgcaatt agattacgaa ggaaactctt ctaccgtaac aaaaggacgt ttatctggtt
    13261 ttggcggtgc gcctaacatg ggacataatc ctggcggtcg tcgtcattct acaccagcat
    13321 ggcaatcact tcgcgacaat gatgatccat tagggaaagg ccaaaaatta gttgttcaaa
    13381 tggttgaaac atatggttca aataaaaaac cagtctttgt cgaagaatta gatgcactag
    13441 ctgttcaaaa acaagcaggc ttagccaatg caccagtgat gatttatagc gaagatacta
    13501 cacatatcgt gacagaagaa ggaattgctt atttgtacaa agcaaaaaca aaagaagaac
    13561 gacaagcagc aattgcagca attggcggtg ttacacctct tggaatgact agcacgaaag
    13621 aatcactaga tagcttgcgt gcagccggca tcgtgaaatt accagaagat ctaggcatca
    13681 aacgtagtga agcgaaacgt tcattattag ctgcacaaac catcgatgat ttgatcgaat
    13741 ggtcagatgg cctgtatgaa ccaccaatga aattcagaac ttggtcataa aattggcgag
    13801 aaaaaccttt tgtcgccatt tacccagatg gaagaagaaa gacgaataag cagaacgtgt
    13861 atgctctgtt ttacaaaaag gggagagatc catggaacaa ttagcttata aatatccaac
    13921 gaaacagccg atcaataaga aaatccatgt aggagtggtc ggttcaggag atttggaaat
    13981 cttgatgttt ccaacagaaa aaacagaatc tactgtaaaa atttgtacag gaagcgatgg
    14041 cttcggcgaa gtatggaaca atgtattaac gagatttttt gatcgttatc caatttcagc
    14101 agatatcgtg atcaatgact tcggtgctac accaggagtt gtgtacctac gattgacgca
    14161 agcaatggag gaactttcag atgaaaaata gttttgtaga attaaacgcc agagaacgag
    14221 tgaacgctct acttgacaat ggacgtgaat tgattgatcc attcgatcaa atcatcgcgc
    14281 caagtttacc agcacaagga atcgtccctg aaagtgatga cgggatcgtt gtcagccgtg
    14341 gcacgatcaa tggcaaacaa gcagtagtaa tcgctatgga aggaaaattc caaggtggcg
    14401 gtatcggtga agtttccggt gcaaaaatcg ttgctgcact tgaacatgtg ttagaagaaa
    14461 ataaaaaagg aaatgagatc tttccagtca ttgtactaga tacaggtggt gttcgtttgc
    14521 aagaagcaaa ctacggactg ttatctattt cagaaatcgg aaatgtaatc attgctctaa
    14581 aacaatatgt accagtcatc ggtttgattc caggacgtgt aggatcattt ggcggtatgt
    14641 cgattacttc agcattaatg agttatttaa tcgcaacaaa aaaagcgaga gtcggtttga
    14701 atggaccaga agttattgaa caagaagcag gcgttcgaga atttgattct agtgacaaag
    14761 acttgatttg gaatacatta ggatctcgtc aacgggtaga atcaaaaatc attgatgaat
    14821 tagtgactga tagtgtagaa gcaatcaaag ctgctgtcat cgcagcaatg gacgaacgaa
    14881 aagacacgca tcgcagtgaa agctcagcat tttatttatc attacttgat tctttagatc
    14941 tttcaaaacc aatggccatt gacgattaca atgaagcaat tgctaaccat caagcaaaat
    15001 caattgactt accagaagta gcagaaggaa atgaaccagc tgcagcaagt gttggttacg
    15061 attggtttgc ggcactaact ggcatgaaaa atccaacttc ttctattagt actgtgtatt
    15121 atggtaaatc aagtaaattc atagaagaag ccatcgtgac atcaatcgta cctgatccta
    15181 aaaacccgat gtaccgtgta cgaaacggtg aagttggttt agttgaaggt ttccgcatgg
    15241 gacaaatttt ggatcacgtt tacgaagaag ataaagacaa aaaagtgaaa cggccaatca
    15301 tcgttgtgat cgatgtacca agccaagcat acggctataa cgaagaattg atcggtatcc
    15361 atgtggcttt ggctaatagt gctgctgctt atgcaaaact ccgtcaagct ggacatccag
    15421 ttattggatt gattgcagga aatgctattt caggtgcatt tttagcacat ggattacaat
    15481 caagccgctt gattgcttta aacaataaag aaattaccgt ccaagcgatg tctaaagaaa
    15541 gtgctgcacg tgtgacaaaa cgttcggttg ctgaaatcga agcagctgct gaaaaagttc
    15601 cagctatcgc ttacgatatc aataattata caaaactagg agcgatctat catttcctag
    15661 aagatatcac cgaccaagct gcttcagaac aaaatgtcag caaagtcatc gaagcaatca
    15721 atgaagccat tcaagatgtc cgcaatacaa atgacacgat gctgacaaat cgatacacaa
    15781 acgaaacagc caaaacattc ggacgtgtcg aaacaaataa agtccgtgcc aaaatggacg
    15841 aagaatggga tgcataaaaa atgattcaag cacatgatat tgtccttttt gataaacatg
    15901 aaatagaagg gttcaccttg cctgaatggg tcgttctaac tgaaaagatg ttcggaacag
    15961 ttcgtcgtgc gtcgacggat cacgcagaaa tactaccgat tggtttacgt ggacctgccc
    16021 gcaatcagag atggtcaaca gaaatctcaa aacatgccgt caaacaagtc attcatccgt
    16081 gggacatcat cgaacaagaa tcgtatagac aagagaaaat catggattat cctgtttatc
    16141 agcaatttca tgctgctaga aacatattat caggatgtaa atggggaatc ggcggcagct
    16201 taggatttga attatcgact gggattccgg cagtaaaaga aaccagtgat tttgatttgc
    16261 tgctgtacgc aaattcaccg attgaattgc caattcaggt aattcaatcg catcctgctt
    16321 tttttgagca attcgatacc caagtagtga ctcggcaagg cggtttttca ctaaaagagt
    16381 atttgagaga gccagagaaa aaaattctat tgaaaacagt cgaaggacct aaattaacca
    16441 aagaactttg gtaataaaga aaaagagaga gaaagagatg tttgaaaaca aaaaaatcaa
    16501 aatcaacgta agtgacttcc agaaagtatc agctgtaacg gctgttatga tgcaaacgat
    16561 attgagcttt gctttggcaa acagccacag cgcaacaaat acagcttttc ttggtacctt
    16621 ttacgtcatc gttaaataca cagcaccaat gtttattttt gcaattgttt ataatatggt
    16681 aaaaacaagt cagcatttgt cttattggga atttttgaaa gaaaagtttt ttgaactagt
    16741 cgttccgtat cttctatgga cgacggctta tctttggcta tttccagcag tccaacaaca
    16801 aactccgtat acggatactg caagcttttt actgaaatat gtcacaggtg atggagcgcc
    16861 tcatttatgg tataccgtta tgatgctgca gatccaattg ttcatgccgt tttttgtttg
    16921 gctcggttac aaagtatttg ctaataaaaa atatgtatgg ccagtattga ttggttcaac
    16981 gattttatat gtcgcatggt atctgttcta tcaaacacaa gtattgactg ggccttatca
    17041 tgacagctgg tacttattag atcggtttgt tgctagtttc atgatttatg gggtagccgc
    17101 atttgtttat cacgaaaaag tctatcaata tttggacaga gtacgttatc ttttcttacc
    17161 tgttgcctta gttatcgcgt tcttttctgt ccgttcattg ctcgcacatc caggagactt
    17221 gagctttgcg aatgcacctt acttgaatac gatccaaagt ttatacagtt tagtcatcat
    17281 ctttgctgtc ttcataggcg cttccaaaat gattgtaaac gattcgccaa aattgccatt
    17341 gttcaaatgg ttgtctgttt atgcttatcg tacgtattta gccaatgtat ttgttttcca
    17401 agtactgtta ttattgttta aagatagttg gctgcagtta ccttcaggag tcatgattct
    17461 tgtagcttat ctaatgacgg caagttgtgc atttgcttta agctggttac ttcatatcat
    17521 ttgggtagct atcaaaaaag gtttcagcaa gtaatagtag gaagaaggag tgagtgtcat
    17581 gataaatgac gaacgaaaaa aagcagtgac tcagcaactc gctgcgttgg tagaggaagc
    17641 attgatcgca gaagtaacat taagtccaaa acctggatta gtggatgcac tggatgcggg
    17701 tgcacattcc gatatggact atgctttatt tcttaagtct gctaagacgc taactccttt
    17761 ttttgaagaa atggctcaaa tagcatggca tcatgctatt gatcaggagt taagagaaag
    17821 aattgctgag attgggcgaa aagcggaaaa ggcaatgctg attgctacaa atggcatcaa
    17881 tacccataaa ggagcgattt gggcaatggg gctattgatc agtgtcattg cacaaaaaat
    17941 cagtatggaa cagccttttt ctttatcgaa aatacttgat gatgtcagca agcttgctgc
    18001 ttttcctgat acaaaatatc gtcataaaaa aaccactcat ggtcataggg tgaagcaaaa
    18061 atatggcgta aatggtgcat atgaagaagc gatattaggg tatccgcata tcaggcttgc
    18121 aattcaagca gctaaggagc tagataatga ttcatatgta cagaaacaaa tgcatatgtt
    18181 gttagttttg atgagttcgc tagatgacac atgcgtattg catcgcagtg atcagaaaac
    18241 gttagtggaa atgcaaaaac ttgctcatgc tgctagtcaa agtgctttac caaatcgtcc
    18301 atttacagca ctgttgcact tttgtcagga aaaccaaatc tctcctggag gaagcgcaga
    18361 tctcttgtct gcaagcttgt tcatgctgaa gatagaggaa ttatggactg ccgacacata
    18421 catttgtccg atgttactca atgactaaat aagacaaaat tgctaaaaaa acagtttatg
    18481 catctggtta ttgactcttc cttttagcaa ggctatcata aaaatagttt tttgtaataa
    18541 aagataacga ggaagacaat gaaaaaaatc aattatttag caatcatttt gttagtgggc
    18601 ctatgtttgt ttgctctgcc gacccaagga ttcgctgtcg cgggtggtca tggtggaggt
    18661 acatctagcg gtcgaagttc gggcagaggt tcatttggtg gtcgttcata tgggggcagt
    18721 tattttggca gtcgttctta cggcagagat tcttatgggt attatggtcc aggtacaggt
    18781 ccttctagtt atgagcaaac gggatttctt gcattagctg ttttgtcagt aggaggggtt
    18841 tctgcttttt ctatcgtcaa gaataaaaga caaaagaaaa ggctgaaagc ggagatattg
    18901 tcccgcatgt caggaacggc taaagaaaag aaagcacagt ttctggaaat ccaaaagata
    18961 ttcatgaaga tccaatccgc ttgggaagaa gaaactcctg aaaaagccag aagctgttat
    19021 acagacaagc tgtttgccga acatcagaaa gttctgcaaa aaaacaaagc agacggtgta
    19081 agaaatcata ctaaaaaaat aactattcag gaagtaggaa attatcgaca gatccacgaa
    19141 aatagttttg ccatccgaat tgatttcaga tgctgtgatt attcagtcga tcgaaaagat
    19201 gggcgattga tctcaggaag taagacacaa cgacagtatt tttctcagat atggtatttt
    19261 gatttttctg agaaagaaaa aagatggcaa gccgatttta ttcagccgat tatgctggat
    19321 tgacaggaaa tatttatgaa cggagttgtg acaaaagcct tgtcgcagct cctttttctg
    19381 aataaacggt gtgccagaga tagcctatcg gcaataaggc acgattttgt aaaatatggg
    19441 aagcgatttt tcaaaatcgc ttcttatcgc tcaaggctga acatctctgt cacaacctct
    19501 ccactaaatg gtgtttaaaa gcttgcatct gcgataataa gaacaaccaa acgaaaaata
    19561 ggaagcacta ttttagttta gttgttctta ttacttgatg caggacagct ttttcacaac
    19621 ctcttttttg tatagatggg caaaattttt gtaataatag gcgtatgtct ttttcgttga
    19681 ggagatgaaa aagcatatgg cttatttttt gtttgctttg cttactttct ttccacttca
    19741 agtggcagaa atatctactt tttattatgt aagcggaacc atatacttga aatcagaagg
    19801 agatcatttc tatgaaaaca agaacaattg aaatacttca cagaatcatg acagcccaat
    19861 atccagtaaa agcaaaacat ctagcaagtg agttcaatgt cagtcagcgg acgatccgtc
    19921 aggaagttct tgaagcgaat atctggttga gcagtaaaaa gcttccggag atccgtacaa
    19981 ttcgcaacaa aggttttttg ttgaagctgg aaaaggaaaa gcaagctcga ttagaaactg
    20041 aaataaaaga tgtgaaagac ggctttttga atcggcgcga acgtattttt gatctagttt
    20101 tagccgttgc ttacagccga atcccgatcc atctaaacaa aaaagaagaa gaatttcaaa
    20161 tctctaaaag tacattagat gaagatatga gacggttacg agcaacactg gtcagttatg
    20221 ggatagagat cgtcagttat ggaaagcaag gactggttta taaaggttca gaacgatcta
    20281 tccgtacgat gatctatgat tttatcaatc agaatctggg aatgaccgac ttctttatga
    20341 atcaagatgc acaattaaca ccggctcaaa aaatctttct ccattatttt cctttggtgg
    20401 aaatcaaaaa aatacaggag atctattcca ataagttgtt gaaacaagaa gatgatattt
    20461 ataaaaaaca aatcctgctt tttacattga tctggatcca gcgaatcagg aaacatgaat
    20521 ttatctctgc cattaattgg aaaaatacag aaataaaaga cagtagcttt tctgcattta
    20581 ttgaccacat gatcgaggaa tttcaattgg acaatgtccc gcaagtagaa agaaattatc
    20641 ttcgtttcac tattgagacg ttcaatgcta gagatatctc caattcaatc gagtgggtac
    20701 aagctcaatt actaacgatc caattgatcc aatttgtaga gaacgagaca cacgtacctt
    20761 tccatctaaa ggaagaagct ttattcgaaa atctctataa gcacatggct gcactgattg
    20821 taagaatcaa gaatcatatc caagtcatga atccattgaa agaaaacatc cgaacaaatt
    20881 atttcccaat ctaccgcgca gtcactcatt ttgttcctac gatcgaagaa gtaattggtg
    20941 gcaagatact ggaggacgag atagcttttc ttgtcatcca cttttcgacg atcgccagtg
    21001 taatcaagcg agacttgaat tatgtttata aatcagtcgt tgtctgtaac catgggatgg
    21061 ctactggaaa tctgttagca gagaatctga aagaaaagtt tcctcagatc gagattactg
    21121 cagttttaag ttcaaaagaa atcgaattag tagataaatt ggatgtcaat ctggttttca
    21181 gcacctttca attgaattat cgaaataagc cgcttttagt tgtcgatccg attttaactg
    21241 agacgaatat cttggttata gaagactttt tgaaaagaaa tgaagaacta cagcggttag
    21301 agcctaacag tcatgaatcg acggagttat tcactcaaat aatagatgtc atgaaagaaa
    21361 gtggtggccg agtagatcga atcatctatg gcaagttaga aaaattattc gaaacaaaca
    21421 atttagaaat caacaaaagg gagatccagc ctatgttaaa agatattttg acggacagca
    21481 atatcatgat caaagaaaaa gccactactt gggaggaatc gattgaacta gctgctagac
    21541 cattggttaa agaaaacatt gtcgaagagc gctatgtcga agcgatgatt cgtgcagtga
    21601 atgattacgg accgtatatc gtgattggca aacacatggc actagcccat gcccgaccag
    21661 aagatggggt caataaatta ggagtcagcg tagctacgat tgagcaacca atcgatttcg
    21721 gtaatcctga aatggatcca gtaaagatta ttttttgttt ggcagcagta gactcctact
    21781 cgcatctgaa catcatgaaa gaactaattg aattgatcaa caacgaagag aagttggatc
    21841 gattgattga atgcacaaat atcgaagatt tcaaacaact gcttttttca gtagttgaga
    21901 aataataaat gatacggggg agaacaaaga tgaacaaatt gaacatttta ttcgtatgcg
    21961 gtgcaggttt aggaagtagt tttgcagcac agatgtctgc tgaggatgtt ttgaacaaat
    22021 taggagtaga agccaaactg gaccattgtg atatttcttc cgcagtttcc atgaaaccag
    22081 atgtgatcat cacagcagaa aattttcgtt cgcagttcga aaagttcgca gttgatccta
    22141 aaacggcgat gatctatttg aaaaatattg tttcaaaaaa tgaaattgaa gaaaaaatca
    22201 ccccagttct gcaagataag ggagttatct aaatacaaaa agaaaagagg gtaaaaa
//
LOCUS       DAZKCE010000045        22076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000045 DAZKCE010000000
VERSION     DAZKCE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.045"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG8KQ_001495"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG8KQ_001495"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9104435.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG8KQ_001496"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG8KQ_001496"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9104436.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG8KQ_001497"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG8KQ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9104437.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG8KQ_001498"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG8KQ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9104438.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG8KQ_001499"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG8KQ_001499"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9104439.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG8KQ_001500"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG8KQ_001500"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9104440.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ACG8KQ_001501"
     CDS             complement(8297..8767)
                     /locus_tag="ACG8KQ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9104441.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ACG8KQ_001502"
     CDS             complement(8813..9238)
                     /locus_tag="ACG8KQ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9104442.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ACG8KQ_001503"
     CDS             complement(9305..9574)
                     /locus_tag="ACG8KQ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9104443.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ACG8KQ_001504"
     CDS             complement(9585..11180)
                     /locus_tag="ACG8KQ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9104444.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG8KQ_001505"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG8KQ_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9104445.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG8KQ_001506"
     CDS             complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG8KQ_001506"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9104446.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="ACG8KQ_001507"
     CDS             15646..16620
                     /locus_tag="ACG8KQ_001507"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9104447.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="ACG8KQ_001508"
     CDS             16790..18190
                     /locus_tag="ACG8KQ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9104448.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="ACG8KQ_001509"
     CDS             complement(18268..18990)
                     /locus_tag="ACG8KQ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9104449.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="ACG8KQ_001510"
     CDS             complement(19069..19575)
                     /locus_tag="ACG8KQ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9104450.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="ACG8KQ_001511"
     CDS             19698..21497
                     /locus_tag="ACG8KQ_001511"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9104451.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21565..>22076)
                     /locus_tag="ACG8KQ_001512"
     CDS             complement(21565..>22076)
                     /locus_tag="ACG8KQ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9104452.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6111 a         4828 c         3993 g         7144 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
    21601 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
    21661 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
    21721 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
    21781 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
    21841 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
    21901 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
    21961 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
    22021 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttg
//
LOCUS       DAZKCE010000046        22035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000046 DAZKCE010000000
VERSION     DAZKCE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.046"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8KQ_001513"
     CDS             <1..288
                     /locus_tag="ACG8KQ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104453.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG8KQ_001514"
     CDS             347..787
                     /locus_tag="ACG8KQ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104454.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG8KQ_001515"
     CDS             799..1467
                     /locus_tag="ACG8KQ_001515"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9104455.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG8KQ_001516"
     CDS             1531..2154
                     /locus_tag="ACG8KQ_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9104456.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG8KQ_001517"
     CDS             2210..2596
                     /locus_tag="ACG8KQ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9104457.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG8KQ_001518"
     CDS             2809..4200
                     /locus_tag="ACG8KQ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9104458.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG8KQ_001519"
     CDS             4811..5179
                     /locus_tag="ACG8KQ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9104459.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG8KQ_001520"
     CDS             5359..6300
                     /locus_tag="ACG8KQ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9104460.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG8KQ_001521"
     CDS             6369..7100
                     /locus_tag="ACG8KQ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9104461.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG8KQ_001522"
     CDS             7244..9424
                     /locus_tag="ACG8KQ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9104462.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG8KQ_001523"
     CDS             9444..9926
                     /locus_tag="ACG8KQ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9104463.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG8KQ_001524"
     CDS             9942..10703
                     /locus_tag="ACG8KQ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9104464.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG8KQ_001525"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG8KQ_001525"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9104465.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG8KQ_001526"
     CDS             12475..12960
                     /locus_tag="ACG8KQ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9104466.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG8KQ_001527"
     CDS             12953..13636
                     /locus_tag="ACG8KQ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9104467.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG8KQ_001528"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG8KQ_001528"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9104468.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8KQ_001529"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8KQ_001529"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9104469.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8KQ_001530"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8KQ_001530"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9104470.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG8KQ_001531"
     CDS             17933..18265
                     /locus_tag="ACG8KQ_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9104471.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8KQ_001532"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8KQ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9104472.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG8KQ_001533"
     CDS             19091..20464
                     /locus_tag="ACG8KQ_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9104473.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8KQ_001534"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8KQ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9104474.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG8KQ_001535"
     CDS             21519..21959
                     /locus_tag="ACG8KQ_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9104475.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7473 a         3814 c         4810 g         5938 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tggga
//
LOCUS       DAZKCE010000047        21984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000047 DAZKCE010000000
VERSION     DAZKCE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.047"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8KQ_001536"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8KQ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9104476.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8KQ_001537"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8KQ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9104477.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8KQ_001538"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8KQ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9104478.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8KQ_001539"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8KQ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9104479.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG8KQ_001540"
     CDS             complement(4333..4647)
                     /locus_tag="ACG8KQ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104480.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8KQ_001541"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8KQ_001541"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9104481.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8KQ_001542"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8KQ_001542"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9104482.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8KQ_001543"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8KQ_001543"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9104483.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8KQ_001544"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8KQ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9104484.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8KQ_001545"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8KQ_001545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9104485.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8KQ_001546"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8KQ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9104486.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8KQ_001547"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8KQ_001547"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9104487.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8KQ_001548"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8KQ_001548"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9104488.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8KQ_001549"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8KQ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9104489.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8KQ_001550"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8KQ_001550"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9104490.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8KQ_001551"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8KQ_001551"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9104491.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8KQ_001552"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8KQ_001552"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9104492.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8KQ_001553"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8KQ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9104493.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8KQ_001554"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8KQ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104494.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8KQ_001555"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8KQ_001555"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9104495.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8KQ_001556"
     CDS             20771..21004
                     /locus_tag="ACG8KQ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9104496.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8KQ_001557"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8KQ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9104497.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>21984
                     /locus_tag="ACG8KQ_001558"
     CDS             21613..>21984
                     /locus_tag="ACG8KQ_001558"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9104498.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLAR"
BASE COUNT         6209 a         4916 c         3885 g         6974 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgt
//
LOCUS       DAZKCE010000048        21828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000048 DAZKCE010000000
VERSION     DAZKCE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.048"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..569)
                     /locus_tag="ACG8KQ_001559"
     CDS             complement(24..569)
                     /locus_tag="ACG8KQ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9104499.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(581..1273)
                     /locus_tag="ACG8KQ_001560"
     CDS             complement(581..1273)
                     /locus_tag="ACG8KQ_001560"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9104500.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1292..1576)
                     /gene="macP"
                     /locus_tag="ACG8KQ_001561"
     CDS             complement(1292..1576)
                     /gene="macP"
                     /locus_tag="ACG8KQ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9104501.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1589..2152)
                     /locus_tag="ACG8KQ_001562"
     CDS             complement(1589..2152)
                     /locus_tag="ACG8KQ_001562"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9104502.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2380..3042
                     /locus_tag="ACG8KQ_001563"
     CDS             2380..3042
                     /locus_tag="ACG8KQ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9104503.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3166..4362
                     /locus_tag="ACG8KQ_001564"
     CDS             3166..4362
                     /locus_tag="ACG8KQ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9104504.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4463..5839)
                     /locus_tag="ACG8KQ_001565"
     CDS             complement(4463..5839)
                     /locus_tag="ACG8KQ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9104505.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5876..6742)
                     /locus_tag="ACG8KQ_001566"
     CDS             complement(5876..6742)
                     /locus_tag="ACG8KQ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9104506.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6831..7856)
                     /locus_tag="ACG8KQ_001567"
     CDS             complement(6831..7856)
                     /locus_tag="ACG8KQ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9104507.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7874..8548)
                     /locus_tag="ACG8KQ_001568"
     CDS             complement(7874..8548)
                     /locus_tag="ACG8KQ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9104508.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8732..9625)
                     /locus_tag="ACG8KQ_001569"
     CDS             complement(8732..9625)
                     /locus_tag="ACG8KQ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9104509.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9699..11396)
                     /locus_tag="ACG8KQ_001570"
     CDS             complement(9699..11396)
                     /locus_tag="ACG8KQ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9104510.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11389..12702)
                     /locus_tag="ACG8KQ_001571"
     CDS             complement(11389..12702)
                     /locus_tag="ACG8KQ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9104511.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12702..16643)
                     /locus_tag="ACG8KQ_001572"
     CDS             complement(12702..16643)
                     /locus_tag="ACG8KQ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9104512.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16752..17210)
                     /locus_tag="ACG8KQ_001573"
     CDS             complement(16752..17210)
                     /locus_tag="ACG8KQ_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9104513.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17508..18026)
                     /locus_tag="ACG8KQ_001574"
     CDS             complement(17508..18026)
                     /locus_tag="ACG8KQ_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9104514.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18028..18644)
                     /locus_tag="ACG8KQ_001575"
                     /pseudo
     CDS             complement(18028..18644)
                     /locus_tag="ACG8KQ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(18641..19429)
                     /locus_tag="ACG8KQ_001576"
     CDS             complement(18641..19429)
                     /locus_tag="ACG8KQ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104515.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(19491..20402)
                     /locus_tag="ACG8KQ_001577"
     CDS             complement(19491..20402)
                     /locus_tag="ACG8KQ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104516.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20443..21777)
                     /locus_tag="ACG8KQ_001578"
     CDS             complement(20443..21777)
                     /locus_tag="ACG8KQ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9104517.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6341 a         4803 c         3771 g         6913 t
ORIGIN      
        1 ttttatcaaa tcactttgtt tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt
       61 ctcaatgccc attcatgtcc aacgggatcg aaacttgcca cggaatcttt gaaaatatgg
      121 agtttgtatc ctagattata cgcatcgact gctgtatgca acacacagat atctgtacat
      181 acgccggtca aataaatatc tgtgatctgg cgttctcgca aacggatgtc tagatctgtt
      241 ccactaaaag cggaataatg ccgtttgtct atccaataaa cattttcctc ttgctcatag
      301 tcttggtata aactttttaa agagccgtac aattcccgtc cacccgttcc tatcacatta
      361 tgcggaggaa ataagcggtt ctccggatga aaggaatctt ttggatcatg cgcatcaatg
      421 gcaaaaacga caaaatcctt ttgatcaata aacttctttg tatgagcagt cagctttttt
      481 tcaattactt gtcctgctgt cccagtagtc agctttccat cctctgcaac aaaatcatat
      541 gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa
      601 attcgatcac catttctgct gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt
      661 gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat gggatattga
      721 actgacgagc tgtttgtcca acagcggctc cttccatttc gcaagctaat gcttcaggga
      781 aattcgttaa gatttctttg attttatctg gcgagtcgac aaatgtatca cctgtaacga
      841 tcaatccttc ttttgcgttc aaattcgttg cttttgctgc tttaaccatt tcactgcgca
      901 aatatgtgct tgcttcataa tatagaggca ttcctggtaa ttgtcctggg ttgtagccaa
      961 atcctgttgc atctgcatca aaataagcta ctttgtctga tataacgatg tcacctactt
     1021 gtaagccttc accgattccg ccagctgaac ctgtattgat caccatgttc acgccgtatt
     1081 gttggatcaa cagacttgtg gtaatagatg ctaagacttt tcctataccg gaacgtacga
     1141 cgatcacttc gtggttacct aatgaacctg aaataaataa agcgcctgcg cgttcccatg
     1201 ataatggttc gcttaggttc tctctcaaaa tttttacttc ttcttccatt gcgccgataa
     1261 taccgatttt catcttttta attccttctt tctatataaa tgcgattgct aaaaaaacca
     1321 cacataacat taggataaca ataataagag atttcatcaa aaaagagttc catttggtcg
     1381 tcttttcccg ttcgctgatc cttgttttcg tgattggttt attctttttg ctttctttac
     1441 gataaaagct ggcaatcttt ttttcttctt gctgataagc tgtttctgct tttcgctgtt
     1501 tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa
     1561 gcggtccttt ggccataagc cgatccctct actttccttt ggtcaatagt tcccagtatt
     1621 gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct
     1681 cttcaagagt tagatgatac aactccagta cttcgtcttc atcttgtgct aaaggattct
     1741 cgaccttctc taccccttgt gcatgataaa tatgaaggac ttcatttgca aaacctggtg
     1801 aaagatacat cgaagtaaga tggctcagcg acttcgcacg atagccagtt tcttcttcta
     1861 attctctagc tgctgtcatt tcgggatttt gtccttctcc tggatcgatt tttccagctg
     1921 gaatctccaa aatcactttt tctaaaggtt ttcgaaattg tttgacaaga agcagacgat
     1981 cttgttcgtc aaaagcaatg atcccgacac cgccaggatg aaataccagt tctctcttcg
     2041 aagttcctcc atctggcaaa cgaacttcgt ctacagcaac atcaataatt tttccgctat
     2101 agatttcttt gcgactgatt gttttttctt caaattgctc attgttcagc atatcttctc
     2161 ctgctttctt tgtaatcata actgcttttt tttcattttt caatttttct caaaaactcc
     2221 ttattagttt accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag
     2281 tccgatactc tttctagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag
     2341 aattcaatac tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg
     2401 tggcttctag cattggtctt gctatttctc tttgtccgaa acaatagttt ttcttttgtt
     2461 ctaggacttt tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg
     2521 aaaaagggaa aacaagaaga aattcctgca ctgactgacg aacttgaaac tcactatgaa
     2581 aaaagcggta tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa
     2641 aatgagatca atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat
     2701 ctccgccatg atccagtcaa agcagcgaaa gctttattca aagaattggt gaaagaacca
     2761 aaccgactgc atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg
     2821 acaaataagt ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa
     2881 ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa
     2941 aaattcgttg ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc
     3001 ttaaagcgtg ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag
     3061 aagactcgga acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac
     3121 attcatgtaa gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg
     3181 actgaaacaa aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg
     3241 ttttcaactc caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa
     3301 gagcaacaga cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct
     3361 aaacagctag cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg
     3421 gctgcccaag caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa
     3481 gatattggtc ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat
     3541 cctgacgaac tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa
     3601 caatcgatct atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt
     3661 tcagtgaaat tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag
     3721 ctttaccaga aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa
     3781 ttgaaaatgg aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa
     3841 actggcgatc agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg
     3901 gataaacgta cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa
     3961 atccgtttga tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct
     4021 acagccattc cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa
     4081 gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat
     4141 tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg
     4201 gatatcgaga cccttcaaac cactcaaaat gatttgatcg aaacaatcca agagacattg
     4261 cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg
     4321 gaagaagatc tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta
     4381 ctaaaacaaa cgcccaacaa cagtttatta caactgttga tgggcgtttt tcttatcttt
     4441 cttctaactt ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc
     4501 caaaataaat gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt
     4561 gcagacgaag catcaataca atacaagtca agatggagac gattggcaat agcggaacga
     4621 atggcacttt gaattctcca gcttttggtt tgccaaacat ttttctcaac ttcagtaagc
     4681 ctagactaac aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat
     4741 tcaatgggaa aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt
     4801 tcggtgtacg atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc
     4861 tgacagcaaa cagtaaacga gcaagtgaat acatcatcga gatcgttacc gtcagtagcg
     4921 ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg
     4981 caaaagccac tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca
     5041 gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca
     5101 catctttttg cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga
     5161 aagcaaaaaa cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg
     5221 gctgccagtt atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac
     5281 caaatttgac atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca
     5341 cgatgtaagt gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt
     5401 ccggattgta cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac
     5461 cttgcatata gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga
     5521 tcaacaacca ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat
     5581 atggcccacc aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa
     5641 tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc tggaccagca ttttgattgg
     5701 ccgcaacccc tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga
     5761 tcaaatcaga cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt
     5821 tcttacgaaa caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa
     5881 ctgttttcat ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa
     5941 cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag
     6001 agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg
     6061 ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca
     6121 caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga
     6181 tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat
     6241 atacaccata accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca
     6301 ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc
     6361 ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat
     6421 ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta
     6481 gccctacaga aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc
     6541 aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa
     6601 cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat
     6661 tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc aacataggga
     6721 ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat
     6781 cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt
     6841 tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt
     6901 gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct
     6961 aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag
     7021 atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact
     7081 ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg
     7141 gcattactaa gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta
     7201 tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt tgcctgactg
     7261 cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta gtcccgcgag
     7321 aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat
     7381 tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc
     7441 aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt
     7501 tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga
     7561 ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa ataattttgc
     7621 aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa
     7681 ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg
     7741 ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa
     7801 aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta
     7861 accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt
     7921 ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt
     7981 catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct
     8041 ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga
     8101 tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca
     8161 tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg
     8221 caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc atgttcatag
     8281 ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata
     8341 cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta atatctagta
     8401 atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc
     8461 caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt
     8521 cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta
     8581 caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa
     8641 acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca agcagcttaa
     8701 atttattatg ttattcttaa taattggtct attatctgtt atttttcttt cgttgttttt
     8761 gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc aatataccta
     8821 gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga
     8881 cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt
     8941 aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct aatgtatttg
     9001 tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca
     9061 cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct
     9121 cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag
     9181 aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata
     9241 aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt tgttcgtaat
     9301 attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc
     9361 ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta
     9421 attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc
     9481 ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag
     9541 ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag
     9601 gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa
     9661 cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga cttcatttta
     9721 gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc
     9781 aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct
     9841 ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg
     9901 ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg cgccatggtc
     9961 tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc atttccatct
    10021 gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc tcctgattca
    10081 taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg gtcttcattc
    10141 cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata tttttcaaac
    10201 tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata atcaaattca
    10261 tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc ttctagttct
    10321 gtggaaacct tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta
    10381 cctaaagtat ctactttggc tactccattt tcttctgtca aaatcgtcac gacaaacccg
    10441 ttgcttacta cttctactat ataatcttgt ccttttgtta aaatcgtctc gccggctcgc
    10501 acttctattt cctgatcata ataagttaga ccaactgtag gcgtatctgt gattttgtaa
    10561 tttttgataa cagcgcttcc gttttcaagg acagaatcga tgaaataagg aataggtgct
    10621 gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa tataagaaat cgtttcttgt
    10681 tctccagctt cattaataat cgttacttgt ctaaggtcgc tttcctctac attcatctgt
    10741 ttatctgctt ctctgatttc gtttttcgga tacaaatgca catctgtatt gtatgtgtct
    10801 ttccatgtag aaccatccca cacgccatcc accatatcag gcatcatcac tggcatcata
    10861 acaaccattg gagacgcgat tctggaaata tgagctggcg aataagtttc taagaaaaga
    10921 tagactttat ctctagaaga aggtgcttct tccgtcactt ggacggtaga gaataccgct
    10981 tctccagctt cattcgttgt ttgtttgtca atacttgtca gtcgatattt taaaaaccaa
    11041 ctttctgtgt gccgtttttg aatccattca atcgcttcgg cagaattaag accgtcttct
    11101 cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat atacttccgt tacgtcaaac
    11161 aaatcaaaat cgactcctgg aatattttct tcaccaaaat ccgtagaaac caatccgcta
    11221 ttcggtcgtt cttccggaac agatgagaaa ccttttttgt gtaatgtaat cgtcacttca
    11281 cttggttttt gtgcgacttc ttcggctttg actagttttc cgttcccgaa aatccctatt
    11341 cccgcagtaa cgagtaacag tacactcatt cccgtgacaa tcattttttt attcattttt
    11401 ttctccccgt tcttttttta ttaataaaac agcacctaac aataaaagaa ttcctagtaa
    11461 agaaatcaac gttcttgctt cattcgtttg cgggaatacg gaccattttc ctgattgacc
    11521 tggattgctt ggaccactcg gctctttcgg tttgtccgtt gtttctttta cggtctcgtt
    11581 ataaacacgt ggtgttgcgg attcataggc agagactgga actttcccgg attctaattc
    11641 agccatctcc tgtccgttaa ctactacata ttgtggttgg ccttttgcat ccacccaaaa
    11701 ttcagggatg atgacctcga ttctcttact tgcttcatcc atctcgtaac cttccacacc
    11761 ttttaattct tcaaaataat aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag
    11821 gccctcttta tccgaaatga atttgcttac atttttgtca ttcaaaggat cattgtctgc
    11881 tggtttgatc cattgatttt tcaagtcatt agcaggagcc atatctaaat aatatttccc
    11941 cccttctatc atctgatata gagcaaaaac agcaccttct aatggttttc ctttttcagt
    12001 tgttcctctt tgacgtccat acttgaagaa gtatggatct cttagataac cgacattttt
    12061 aggatagata tgaatctctt gcagttcctc ggttgattct gtcgggtgat aaattggcaa
    12121 aatagcagca attgggatag ctgtctgatc gatatcaaca tccaatccaa cttctggatc
    12181 aatcgattct tcgaaaatca aataaactgc atcccgatct tggtttttct taggtgcttt
    12241 gatacgtgca atcccgtctc ctaatccgcc aaccgtatct ttcccagttg ttaccgaagt
    12301 ccctatttgg acaagccttt cttctgtgat tagctgacgg atattgtttc tgttcgtttc
    12361 agcgatttgc tggacgaatt ctttaggagt catgttttca gcagcttttt tttcatgata
    12421 ataatcggtc atgtcgtaaa tggcaaatgt cgctccattt agtggcaccg tctgtgaaat
    12481 gatatctgct gtttcccctg cttcctcaga gatagccaag ccatcatttt catggtattc
    12541 aaattgatcg ttataatcga tatctcgaat catccgttta tgcaacacga attccacgac
    12601 atcttcccct gattcttctg ccgacatcgc atgtggaaga aggactgctg caagcggtag
    12661 taaaacaagt aattgaagca tcaaacacaa cagctttttc attaccttgc tccctccttt
    12721 ttagattgtc tgacaaacaa accataggct gctcctgcaa gcgccatgaa gaatgcagca
    12781 agcaggatat agaataatcg tccggatcct cctgtagcag gcaacggatt ttcaggatcg
    12841 ttcccgacac taagctgtaa tctgattcga tttgtctcca aaagttcata ttctaactct
    12901 tgctgtagat caggcgtctc tgcttcttcg tttggataat acgaaatctc aacttttagt
    12961 gtacccgctt cttttcctgg aactacccga tcattttcta tgtcaggttc ttctcgataa
    13021 ctaatttcta aaataaagta acctggaagt tttctatagc tgtcaggtga cttcacttcc
    13081 ttgatccgat agaccccttc caacaagtct gaagcattga atagggaaga atcgtttgga
    13141 tctggtgcca agcctccctc gatgatttca tactctcctg cactattttg tttctcaatt
    13201 gaaaattctg cgccttcaag acgtcggcta ttcatactgt caattttcaa aagttccaaa
    13261 tcgattggcg ctagttcatc ttctaaggtt acttgtagta caccatcgat gatttcatag
    13321 ccttccggca agggagcagt ttcgtcttcg atgaccgtat cttcaatgat aaacttacct
    13381 tcttcattca gtgcaaactg gaacacttga tcagatagcc gatacccatt tggtgcgaca
    13441 gattccgaga cctcatattc accaatagac agatgcagag gttggtcgta atttaaatcg
    13501 acatactttt ctactcctac ggcacgagaa tagaagtatc cgatcccagg caaacgtttc
    13561 gattcttctg gttctaacgg attaccagtg tatgctcgat acagcgggaa ggaagaatcc
    13621 cccactttcc tgatatcgaa acctgcacca tctaatgtat ggccttcatg gtctaatttt
    13681 ctaactcgga tctcccgata atcaaacgta ttgtaagcag tgaatgctac ccataaacga
    13741 tcctcttcat ctgttgtata cgtgagatat tgccaatttt cttcacctgt ttgcttgagt
    13801 tggatagtcc ctatagcatt atcgtaaagt cgcaagatcc atgtgtcatt ttcatcaggt
    13861 tctttgaaac cttctgggtt ctggctctca atgattttgt attctgcata gttggtagtt
    13921 tcttcactat ccttataaac aaatgaatct ggctctccga ttggatgaat aactttctca
    13981 ccatccacga ttacatattt cccgtcttca tctactgaat agaacgtgag tctccctttc
    14041 tcttcctcta cttcgttcac accagaaagc cctttcgcta ctggatatcc gatttccagt
    14101 ccgttaccat ctgtttcata aagttcaaac tctgcgccat tgattggcat attactgcct
    14161 tcaattagtt tattgatatt catgtggatt ggcttgaatt catttcgcat ttccagtttg
    14221 acatacaact catcatactc tgtgccttct atgttaggct cttgtacaac agaaagcttg
    14281 tgatattctc cttcataata ggaattcgga tctttacctt ccggtccgcc gactctcgaa
    14341 ccagatgtat attcatctac aagctcgtag tgacctaaca tctcatcttc gttggctgca
    14401 tattttttga tttggaattg atacgtctca tctactaact gataacgtgg tggtccagcg
    14461 atctctctga tccaaaatat gtcgctgact gctccagttt catctcctaa aatcggcatc
    14521 ttcgtttcaa agatcgctat tccgtccgtg gtagttttac ttgtttcgat tggttcggct
    14581 tccccactct ctggattttg gtcatataat gcaaattcga ctccagccaa cggctcgcca
    14641 ttttgaccct tcttgctgac ttggaaatta cggcctttat gttcgtaacg aatggaagaa
    14701 gatgggaaat acatccgtcc agtagtgata tcattctcat aatcctccag gttctgaata
    14761 taggccatat tgttcactgt gcaaaaattt ccgtcttgag ctcccgcatc aagttttact
    14821 tggtacttga tcctgaccat ttccccttca cctaaagaaa tcggagaaaa tttataagct
    14881 tggttttccc gtccgacata cgttgtcgga gtcgccacta cttctggtgg ggcttgtcca
    14941 tgacgttctt catcccatat ttctggctct tggtacttca tgccgttttc tcctggatct
    15001 agatggtaaa actgaatatc tgccgatctg ccagctccaa atggtgagac attaaaacca
    15061 gtttcatcat aaagtgtggc ttgctggatc gttctcttga aggtagaaga gatatcctcc
    15121 aaggctgtaa caatgtcatt cttgttgttc gcattgtaat atcgcggtgt tacgccatct
    15181 tcttctaaat cagcaatatt tttcaataca tttcgtccaa tagcatctag tcgttgtctc
    15241 ccgctaacgt ttccatcgat tcctaaacca atggagaaga tacttggcgg gttctcttga
    15301 tacttgtttt tcagccaatg atgataagca actgtgttga tcgcagatga cttttcttgc
    15361 gaagtaggac tgccattcgt attgttatta gaagaaccat caccaaccca gtgagtcgct
    15421 ttattatctc cttcttttac ctctccataa cgatagtgta catttacttc atcttctgga
    15481 cggttaaagt cagatgcata ggtaactgga tgagtaaacc ctgcattaga agtattccca
    15541 gaaaactcct caaaatttct gaacaaaacg tcattatctt cagcaatctt atcttccatc
    15601 acagtccagt tgctccaagc tccattagtg ctcgtacgat aacttgactg aatcggtgca
    15661 taactgaagg tcggtgtccc atctcccaca acgattagga ttttttctgg atttctattt
    15721 tctccgccat tgtctttata caatgtttca tagccatttt taagccctag tgtaagcgga
    15781 gttcctccga ttggtgtacg atttaatgct gtattctcac ttcccgtcaa acgattgata
    15841 tcatttgtca atggaaagat gtccgaactg atttgctcat ggttattgat agctgtacta
    15901 ttatggtaaa aattcaccat accgattcga atatccagac tcgggtcaga gagcagatct
    15961 ttactcattt cgtttactgc ctcaatcaac gcagcatttt tagtttgact attggtccca
    16021 gcggttaatt cactcatact cgctgactta tctagaacaa aaacgatatc gattggactg
    16081 atttcctttt tctcgccccc gatgacatct agatagacat tgtaaagggt tgttgcatct
    16141 tcaggatgta gatcagatgc tgtgctgctt ggcagaacag gttctaccca ttttttgatg
    16201 tacgccccgt tataatccaa gtaactgttt tcaaaattcc gttcatttcc ccaaagctga
    16261 acggtttgag ctggaacgtt tctatcttct tcgcctgcag ttgatccatt attagtagca
    16321 tagtcgtaat ttcgccagtt cgctcccccg tttgtataat cactatcatc gatcaagttc
    16381 tctccgtcat atggcgagcc tttgtctcct tgatagcgtt cattgctttg tgtccaattt
    16441 cctaacactt ttgtatagtc attcgttgta aacttcgggt agcgatgctg ttcgttttca
    16501 acttctccgc taggatagat atattcatag gggtcttgcg tagctgttgg cccaagacgc
    16561 tccaaaatac cgttgaataa ctggcgttga atgcgctgga gtgcttgacc gctgggtacg
    16621 attggttcgt ccagcttttc catctcattt tcttcgctac gttgaatttc agcggaatct
    16681 tctgtatcag tttcattttc ttgtaattct tggttatcta ttctttcatc aaccaccact
    16741 tgaagaggta gttacccttc gttcacatcg gggatctttt ctgtttcaaa aatcaatgtg
    16801 ccggtttctg cttcttttga ataaactttt ccgacatacc attcttgacc atcgatcgtc
    16861 tcgactttta ggtcttctcc atcttcatca taggaggata gacctgttcc acgaacgttt
    16921 tttacggtac caatcccggc ggctgccgca tcgattgcta aacgtattct accttcattg
    16981 gcactttcct ttttttcaaa agccagatgc cattcaaggg tttcttcttt ttcaatatac
    17041 ttcccttcaa tttttgcata gtcctcatct aagaatacct catcctcctt tgtttctgtt
    17101 gactcagcag ctacatccag ggtcgtcata tgatagactg actggaaata tggtccaaat
    17161 agcaaactga cagttactaa ctgcaaaagc ttcaatttta accctttcac atccttatac
    17221 tcctttcaaa aatatttgtc ttctttcaca acaatcaaca aaacaatgcc catctaatta
    17281 taatttaatt taattttaat tttaaatcaa atgatatcct ttttagttaa aaaacaccaa
    17341 ttatacaaca tttattatta aaattaaatt ataaaatttt ataacgttct catttaacaa
    17401 aacttaagtt attaagacaa atgaataaac aactacgctt tcctcgaaac aaaaaaataa
    17461 agcacaaatg cgcatttacg cactcatgct ttattcgtac aattaattca gataacggca
    17521 gcgcatagga tgccaataac acttcctaaa atagctcctc ctaaaatgtc agtaggataa
    17581 tgtaaaagta aaaaaatcct cgaataagcg atcaaacaag caaataaaaa ggctaatatt
    17641 cctgcaactg gacttaattc gaacaacgca gctactgata aaaaggctag cgctgtatgt
    17701 cctgaaggaa aagaagaagt cttcggttta cttgtgtaaa ggacaagccg acgatttttt
    17761 tcataaggtc ttttccgtct tgtcgttttt ttgataaaaa gcaaaccaac aaaaaaatag
    17821 atcaaactgc cgataagaat ggctagacca tattctctag tatcaggtat caagactaaa
    17881 acagcagcaa ggagcaacca aataaaacca ccattagaga tattactgat aaatttcaaa
    17941 aaattgattt ttcgactatc cgttttccgt tgcttggcga acttgttgat aatactatca
    18001 tcgattttag caatcaaacg caacatgtta ttcctccttt ttaagaacct tttcttgcgt
    18061 acgatggata aaaagcagga cttgaaaata aaacaagaaa ctgaaaccta ataaagcaaa
    18121 atagattggt cctgcaaacc agaatgcgac aagaaggatc aacgtgcctg ccaaatagaa
    18181 aagcgcgctt ttgaaatcat taaaaaacaa gaaaaatgca gtaatccaag aagcttcttt
    18241 ttgttccagt tgaagcaaga ttttgtaact aaagagaagc gcgcttaata gaagcaaaaa
    18301 caacgtagta tacatcaatg tatagccaat tcccccaccc caatagcgga aaatctgata
    18361 atcgatcaat agaagaagaa aataaacact tgctaaacta tcttgctttc cataaagaga
    18421 gaagttctca cgaaactttt tccagaatag tgaaccgagc tttttctctt catttttttg
    18481 taatgataaa tcatatacag aactgttcga cttgcagttc ctaatgtgaa aacaggcaag
    18541 acgcacagga accatagtag ctgtaaatgg ataagacgtg gaatccattc aatgatttct
    18601 aagaaataat cattggcttt tttaccaatc gatcgattca tcatcctttt gaacctcctg
    18661 tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga
    18721 tcaagacaag tgttgctcct gccatcagat aattccattg tgtcgttgct tggttctgaa
    18781 aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc ttggatatat aacagaggac
    18841 ctaagaaatc attccaagct gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt
    18901 tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg attcgctccg tcgatttcag
    18961 cagcttcgat caattcatta ttgatcgata agtaaaattg acgcatcaga aatatattga
    19021 acgcattgcc gaagaacgat ggaacgatca ggggcaaata agtacctacc cagccgatct
    19081 tggagaataa tacatattga gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa
    19141 gaaccaaagc aaacatcagc tttttcccag gaaaatcaag cttcgcaaat ccataagcaa
    19201 taaaagaatt tgaaaggaca ttaccgatca ctactagcac agtgatgaat aatgtatttc
    19261 ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag atagttttcc catacgattt
    19321 tttctgggaa aaaggtcgga ggataacgaa cgatttcttc cattggtttc aatgaagtaa
    19381 acaccatcca aagcaatgga atgatcaaaa tgattcctat tactgtcatg aggacataac
    19441 ttagtatttt tttgtttgcc gattcacact ggtctttttg aaataatcca tcatttgccg
    19501 tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa cgtcagaacc
    19561 aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc aaaagctacg
    19621 aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg tccgcctgtt
    19681 ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat cgtagaggtg
    19741 atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt gaactttccc
    19801 caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac attttgtaat
    19861 gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa aatcaacgca
    19921 ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa ccagcccaga
    19981 aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat gtagactgct
    20041 actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat tcctttgatt
    20101 ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag
    20161 atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg aacgaacatt
    20221 cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata atcggtgaaa
    20281 ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc gataataaaa
    20341 ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga taaacgcttc
    20401 aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat tttgcttgac
    20461 taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt ttgttcctaa
    20521 aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac
    20581 tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca tttttccttt
    20641 atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg ccatgatatc
    20701 aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt tgatgaattc
    20761 ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc cgccacccca
    20821 cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa aatcaaaatc
    20881 atccggtgca ttttccatca aactagagta ataattgata ttttgggcac gcatggctac
    20941 taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta atttattgat
    21001 cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt ccactttatt
    21061 tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt
    21121 tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc gtgtaaattc
    21181 ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc aagttgtcgg
    21241 tagatcttgt tcactgattc cagcttcttt aaatatcttt ttattataaa aaatgacttg
    21301 tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg tatcccaaag
    21361 ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat tagttgcttg
    21421 ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat cgggtgcatt
    21481 tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct gatacttata
    21541 atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt cgatagcttc
    21601 ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc catcacttgt
    21661 tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg atccgcatcc
    21721 tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt ttttcatatt
    21781 ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgata
//
LOCUS       DAZKCE010000049        21031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000049 DAZKCE010000000
VERSION     DAZKCE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.049"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8KQ_001579"
     CDS             complement(<1..563)
                     /locus_tag="ACG8KQ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFP9104518.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8KQ_001580"
     CDS             complement(750..1400)
                     /locus_tag="ACG8KQ_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104519.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8KQ_001581"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8KQ_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9104520.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8KQ_001582"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8KQ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9104521.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8KQ_001583"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8KQ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9104522.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8KQ_001584"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8KQ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9104523.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8KQ_001585"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8KQ_001585"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9104524.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8KQ_001586"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8KQ_001586"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9104525.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8KQ_001587"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8KQ_001587"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9104526.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8KQ_001588"
     CDS             12268..13770
                     /locus_tag="ACG8KQ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9104527.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8KQ_001589"
     CDS             13772..14509
                     /locus_tag="ACG8KQ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9104528.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8KQ_001590"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8KQ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9104529.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8KQ_001591"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8KQ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9104530.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8KQ_001592"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8KQ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9104531.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8KQ_001593"
     CDS             17805..18440
                     /locus_tag="ACG8KQ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9104532.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8KQ_001594"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8KQ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8KQ_001595"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8KQ_001595"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8KQ_001596"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8KQ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9104533.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         6195 a         4300 c         3730 g         6806 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa a
//
LOCUS       DAZKCE010000050        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000050 DAZKCE010000000
VERSION     DAZKCE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.050"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8KQ_001597"
     CDS             291..1430
                     /locus_tag="ACG8KQ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9104534.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8KQ_001598"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8KQ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9104535.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8KQ_001599"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8KQ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9104536.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8KQ_001600"
     CDS             3334..4302
                     /locus_tag="ACG8KQ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9104537.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8KQ_001601"
     CDS             4415..4693
                     /locus_tag="ACG8KQ_001601"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104538.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8KQ_001602"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8KQ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9104539.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8KQ_001603"
     CDS             5150..5905
                     /locus_tag="ACG8KQ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9104540.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8KQ_001604"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8KQ_001604"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9104541.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8KQ_001605"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8KQ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9104542.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8KQ_001606"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8KQ_001606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9104543.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8KQ_001607"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8KQ_001607"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9104544.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8KQ_001608"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8KQ_001608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9104545.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8KQ_001609"
     CDS             10642..11184
                     /locus_tag="ACG8KQ_001609"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9104546.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8KQ_001610"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8KQ_001610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9104547.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8KQ_001611"
     CDS             12777..13679
                     /locus_tag="ACG8KQ_001611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9104548.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8KQ_001612"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8KQ_001612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9104549.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8KQ_001613"
     CDS             15122..15544
                     /locus_tag="ACG8KQ_001613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9104550.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8KQ_001614"
     CDS             16013..16243
                     /locus_tag="ACG8KQ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9104551.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8KQ_001615"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8KQ_001615"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9104552.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8KQ_001616"
     CDS             17654..17830
                     /locus_tag="ACG8KQ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9104553.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8KQ_001617"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8KQ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9104554.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8KQ_001618"
     CDS             18325..18708
                     /locus_tag="ACG8KQ_001618"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104555.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8KQ_001619"
     CDS             18877..19761
                     /locus_tag="ACG8KQ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9104556.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8KQ_001620"
     CDS             19875..20111
                     /locus_tag="ACG8KQ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9104557.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8KQ_001621"
     tRNA            20199..20279
                     /locus_tag="ACG8KQ_001621"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8KQ_001622"
     tRNA            20286..20357
                     /locus_tag="ACG8KQ_001622"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKCE010000051        20150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000051 DAZKCE010000000
VERSION     DAZKCE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.051"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1648)
                     /locus_tag="ACG8KQ_001623"
     CDS             complement(89..1648)
                     /locus_tag="ACG8KQ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9104558.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG8KQ_001624"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG8KQ_001624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9104559.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="ACG8KQ_001625"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="ACG8KQ_001625"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9104560.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="ACG8KQ_001626"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="ACG8KQ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9104561.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="ACG8KQ_001627"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="ACG8KQ_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9104562.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="ACG8KQ_001628"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="ACG8KQ_001628"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9104563.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="ACG8KQ_001629"
     CDS             9410..10465
                     /locus_tag="ACG8KQ_001629"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9104564.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG8KQ_001630"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG8KQ_001630"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9104565.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="ACG8KQ_001631"
     CDS             11952..12080
                     /locus_tag="ACG8KQ_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9104566.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="ACG8KQ_001632"
     CDS             12520..12708
                     /locus_tag="ACG8KQ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9104567.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="ACG8KQ_001633"
     CDS             12786..13799
                     /locus_tag="ACG8KQ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9104568.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="ACG8KQ_001634"
     CDS             complement(13866..14900)
                     /locus_tag="ACG8KQ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9104569.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17240
                     /locus_tag="ACG8KQ_001635"
     CDS             15015..17240
                     /locus_tag="ACG8KQ_001635"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9104570.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17258..19141
                     /locus_tag="ACG8KQ_001636"
     CDS             17258..19141
                     /locus_tag="ACG8KQ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFP9104571.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19122..20096
                     /locus_tag="ACG8KQ_001637"
     CDS             19122..20096
                     /locus_tag="ACG8KQ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9104572.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6779 a         3703 c         4330 g         5338 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc actacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtctacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaagaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17101 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17161 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17221 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17281 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17341 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17401 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17461 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17521 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17581 tttcacgaca agccctgaga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17641 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17701 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17761 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17821 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17881 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17941 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    18001 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18061 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18121 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18181 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18241 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18301 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18361 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18421 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18481 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18541 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18601 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18661 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18721 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18781 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18841 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18901 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18961 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    19021 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19081 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19141 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19201 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19261 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19321 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19381 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19441 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19501 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19561 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19621 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19681 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19741 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19801 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19861 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19921 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19981 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    20041 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20101 ctttagatta ttatttgaaa aacggagttt acaagacaaa gctgctttat
//
LOCUS       DAZKCE010000052        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000052 DAZKCE010000000
VERSION     DAZKCE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.052"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8KQ_001638"
     CDS             complement(<1..210)
                     /locus_tag="ACG8KQ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9104573.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG8KQ_001639"
     CDS             complement(216..446)
                     /locus_tag="ACG8KQ_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104574.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG8KQ_001640"
     CDS             1063..1725
                     /locus_tag="ACG8KQ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104575.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8KQ_001641"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8KQ_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9104576.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG8KQ_001642"
     CDS             5145..5459
                     /locus_tag="ACG8KQ_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9104577.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG8KQ_001643"
     CDS             5472..5846
                     /locus_tag="ACG8KQ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9104578.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG8KQ_001644"
     CDS             5856..6200
                     /locus_tag="ACG8KQ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9104579.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG8KQ_001645"
     CDS             6271..7623
                     /locus_tag="ACG8KQ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9104580.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG8KQ_001646"
     CDS             7683..8825
                     /locus_tag="ACG8KQ_001646"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9104581.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG8KQ_001647"
     CDS             8850..9104
                     /locus_tag="ACG8KQ_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104582.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8KQ_001648"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8KQ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9104583.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG8KQ_001649"
     CDS             10541..10678
                     /locus_tag="ACG8KQ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9104584.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG8KQ_001650"
     CDS             10683..10904
                     /locus_tag="ACG8KQ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104585.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG8KQ_001651"
     CDS             10917..11417
                     /locus_tag="ACG8KQ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9104586.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG8KQ_001652"
     CDS             11514..13361
                     /locus_tag="ACG8KQ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9104587.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG8KQ_001653"
     CDS             13364..14260
                     /locus_tag="ACG8KQ_001653"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9104588.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG8KQ_001654"
     CDS             14310..14699
                     /locus_tag="ACG8KQ_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9104589.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG8KQ_001655"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG8KQ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_001656"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_001656"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9104590.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG8KQ_001657"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG8KQ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKCE010000053        18757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000053 DAZKCE010000000
VERSION     DAZKCE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.053"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..2146)
                     /locus_tag="ACG8KQ_001658"
     CDS             complement(2..2146)
                     /locus_tag="ACG8KQ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9104591.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2503..3876)
                     /locus_tag="ACG8KQ_001659"
     CDS             complement(2503..3876)
                     /locus_tag="ACG8KQ_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9104592.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3876..4271)
                     /gene="rbsD"
                     /locus_tag="ACG8KQ_001660"
     CDS             complement(3876..4271)
                     /gene="rbsD"
                     /locus_tag="ACG8KQ_001660"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9104593.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4299..5216)
                     /gene="rbsK"
                     /locus_tag="ACG8KQ_001661"
     CDS             complement(4299..5216)
                     /gene="rbsK"
                     /locus_tag="ACG8KQ_001661"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9104594.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5324..6337)
                     /locus_tag="ACG8KQ_001662"
     CDS             complement(5324..6337)
                     /locus_tag="ACG8KQ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9104595.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6418..7017)
                     /locus_tag="ACG8KQ_001663"
     CDS             complement(6418..7017)
                     /locus_tag="ACG8KQ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9104596.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7203..7904
                     /locus_tag="ACG8KQ_001664"
     CDS             7203..7904
                     /locus_tag="ACG8KQ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104597.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7914..10625
                     /locus_tag="ACG8KQ_001665"
     CDS             7914..10625
                     /locus_tag="ACG8KQ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9104598.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11076..12704
                     /gene="treC"
                     /locus_tag="ACG8KQ_001666"
     CDS             11076..12704
                     /gene="treC"
                     /locus_tag="ACG8KQ_001666"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9104599.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12764..13945)
                     /locus_tag="ACG8KQ_001667"
     CDS             complement(12764..13945)
                     /locus_tag="ACG8KQ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9104600.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14197..15720
                     /locus_tag="ACG8KQ_001668"
     CDS             14197..15720
                     /locus_tag="ACG8KQ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9104601.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15789..16517)
                     /locus_tag="ACG8KQ_001669"
     CDS             complement(15789..16517)
                     /locus_tag="ACG8KQ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9104602.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16530..17810)
                     /locus_tag="ACG8KQ_001670"
     CDS             complement(16530..17810)
                     /locus_tag="ACG8KQ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9104603.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17951..18553)
                     /locus_tag="ACG8KQ_001671"
     CDS             complement(17951..18553)
                     /locus_tag="ACG8KQ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9104604.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
BASE COUNT         6049 a         3452 c         3676 g         5580 t
ORIGIN      
        1 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
       61 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
      121 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
      181 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
      241 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
      301 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
      361 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
      421 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
      481 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
      541 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
      601 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
      661 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
      721 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
      781 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
      841 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
      901 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
      961 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
     1021 cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
     1081 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
     1141 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
     1201 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
     1261 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
     1321 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
     1381 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
     1441 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
     1501 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
     1561 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
     1621 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
     1681 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
     1741 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
     1801 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
     1861 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
     1921 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
     1981 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
     2041 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
     2101 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
     2161 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
     2221 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
     2281 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
     2341 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
     2401 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
     2461 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
     2521 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
     2581 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
     2641 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
     2701 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
     2761 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
     2821 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
     2881 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
     2941 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
     3001 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
     3061 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
     3121 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
     3181 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
     3241 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
     3301 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
     3361 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
     3421 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
     3481 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
     3541 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
     3601 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
     3661 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
     3721 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
     3781 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
     3841 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
     3901 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
     3961 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
     4021 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
     4081 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
     4141 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
     4201 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
     4261 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
     4321 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
     4381 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
     4441 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
     4501 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
     4561 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
     4621 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
     4681 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
     4741 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
     4801 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
     4861 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
     4921 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
     4981 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
     5041 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
     5101 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
     5161 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
     5221 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
     5281 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
     5341 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
     5401 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
     5461 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
     5521 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
     5581 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
     5641 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
     5701 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
     5761 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
     5821 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
     5881 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
     5941 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
     6001 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
     6061 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
     6121 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
     6181 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
     6241 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
     6301 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
     6361 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
     6421 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
     6481 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
     6541 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
     6601 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
     6661 aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca tattatctgt
     6721 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
     6781 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
     6841 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
     6901 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
     6961 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
     7021 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
     7081 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
     7141 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
     7201 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
     7261 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
     7321 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
     7381 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
     7441 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
     7501 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
     7561 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
     7621 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
     7681 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
     7741 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
     7801 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
     7861 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
     7921 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
     7981 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
     8041 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
     8101 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
     8161 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
     8221 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
     8281 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
     8341 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
     8401 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
     8461 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
     8521 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
     8581 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
     8641 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
     8701 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
     8761 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
     8821 ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
     8881 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
     8941 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
     9001 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
     9061 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
     9121 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
     9181 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
     9241 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
     9301 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
     9361 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
     9421 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
     9481 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
     9541 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
     9601 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
     9661 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
     9721 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
     9781 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
     9841 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
     9901 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
     9961 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    10021 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    10081 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    10141 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    10201 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    10261 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    10321 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    10381 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    10441 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    10501 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    10561 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    10621 aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa acaaacggca
    10681 aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
    10741 ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat actcaaataa
    10801 aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa agcttttgaa
    10861 caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat agacaaatac
    10921 atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata aagataaatt
    10981 ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata taagttgtgg
    11041 taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac aaagtcattt
    11101 accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga gatttaaaag
    11161 gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt tggctaaacc
    11221 ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac acagccatcg
    11281 atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca aagaaatacc
    11341 ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat gaatggttca
    11401 aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga gaagagccaa
    11461 cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat acggggaaat
    11521 attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac caagcagtaa
    11581 gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa ggattccgtt
    11641 tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa gaaaatgatg
    11701 gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa ttgaaccaag
    11761 caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct acgacgatcg
    11821 aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc tttaatttcc
    11881 atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca tttgattttg
    11941 cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt aacggctgga
    12001 atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt gtagctatcg
    12061 atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg aaccgtggga
    12121 cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat tcatcgattt
    12181 ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa caaggattca
    12241 cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct cgaacaccca
    12301 tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg ttaaaagttg
    12361 gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat tctattttgt
    12421 cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca caaggagatt
    12481 atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa tttgaaggac
    12541 agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc atcccagaag
    12601 gattcacaga agccaatatt cttatcgata attattccga tactgttctt catcaaagtc
    12661 tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa aacacaaaac
    12721 gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta aaataatctt
    12781 cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta ttgtagcgtt
    12841 gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag ataggaatac
    12901 caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg attaagatcg
    12961 taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag tcaccgtgga
    13021 gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta gagtcttgga
    13081 atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga atgatacata
    13141 acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt aagaaacaaa
    13201 cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga aatgaaccca
    13261 attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct ggtgggacaa
    13321 tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa gcgacaattg
    13381 gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg ctgatccgtt
    13441 gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc acgtcgtcaa
    13501 tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa aattgtcgga
    13561 gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc tttgcctgtt
    13621 cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa ataatcacat
    13681 aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg atgactggtt
    13741 ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta gcgataatga
    13801 ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc agcgcaagta
    13861 agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg atcaaacgat
    13921 tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataaat tcattataga
    13981 caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg tcaatcattc
    14041 atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt aaccatatga
    14101 taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg aatcggaaca
    14161 ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat aggtatattg
    14221 gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa agccgaggac
    14281 agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt tacaaaagca
    14341 atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga ttcccacgat
    14401 tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt tgtttataat
    14461 gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga aggcaatgtc
    14521 catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga aggacatgat
    14581 catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca tgtatggcta
    14641 gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat tgaagcttac
    14701 ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa acttcaagca
    14761 ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt tgtcacgcaa
    14821 catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc gattgctggt
    14881 ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca ctttgttaaa
    14941 gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat tgccagaacg
    15001 ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg attgacgaat
    15061 gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct cgaagcgttg
    15121 aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa aaaagaaaag
    15181 agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct ttcagattgg
    15241 gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga tcctgttttt
    15301 gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga atattatacg
    15361 aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat ggcatttacg
    15421 tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga aatcttgacc
    15481 tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa ggaaagtggc
    15541 gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc cgaacatttt
    15601 catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga gaattggcca
    15661 acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct tttgcactga
    15721 taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc
    15781 ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg
    15841 tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata
    15901 acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc
    15961 tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc
    16021 gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct
    16081 attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg
    16141 atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt
    16201 tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt
    16261 tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg
    16321 tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca
    16381 ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc
    16441 atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa
    16501 atataaatgg gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa
    16561 aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca
    16621 tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt
    16681 tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg
    16741 gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct
    16801 tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta
    16861 tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc
    16921 atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt
    16981 agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa
    17041 gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt
    17101 tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa
    17161 tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt
    17221 ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta
    17281 ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag
    17341 tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac
    17401 caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag
    17461 acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa
    17521 aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc
    17581 gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct
    17641 atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa
    17701 gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag
    17761 atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc
    17821 tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta atttttcagg
    17881 gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt
    17941 agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt
    18001 ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa
    18061 agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg ctgatcgcct
    18121 tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc
    18181 catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga
    18241 tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca
    18301 gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat
    18361 aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa
    18421 cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt acttggacat
    18481 acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa
    18541 ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa
    18601 cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa
    18661 tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt
    18721 tatgacaagt ttgtgctttt tcgagtgatt tatgtta
//
LOCUS       DAZKCE010000054        18532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000054 DAZKCE010000000
VERSION     DAZKCE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.054"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..774
                     /locus_tag="ACG8KQ_001672"
     CDS             127..774
                     /locus_tag="ACG8KQ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9104605.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    903..1119
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1167..3812
                     /locus_tag="ACG8KQ_001673"
     CDS             1167..3812
                     /locus_tag="ACG8KQ_001673"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9104606.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4122..5435
                     /locus_tag="ACG8KQ_001674"
     CDS             4122..5435
                     /locus_tag="ACG8KQ_001674"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9104607.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5425..6078
                     /locus_tag="ACG8KQ_001675"
     CDS             5425..6078
                     /locus_tag="ACG8KQ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9104608.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6133..6825
                     /gene="radC"
                     /locus_tag="ACG8KQ_001676"
     CDS             6133..6825
                     /gene="radC"
                     /locus_tag="ACG8KQ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9104609.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6975..7175
                     /locus_tag="ACG8KQ_001677"
     CDS             6975..7175
                     /locus_tag="ACG8KQ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9104610.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7429..9108)
                     /locus_tag="ACG8KQ_001678"
     CDS             complement(7429..9108)
                     /locus_tag="ACG8KQ_001678"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9104611.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9110..9322)
                     /locus_tag="ACG8KQ_001679"
     CDS             complement(9110..9322)
                     /locus_tag="ACG8KQ_001679"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9104612.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9715..11445
                     /gene="efrA"
                     /locus_tag="ACG8KQ_001680"
     CDS             9715..11445
                     /gene="efrA"
                     /locus_tag="ACG8KQ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9104613.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11442..13202
                     /gene="efrB"
                     /locus_tag="ACG8KQ_001681"
     CDS             11442..13202
                     /gene="efrB"
                     /locus_tag="ACG8KQ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9104614.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13297..13494
                     /locus_tag="ACG8KQ_001682"
     CDS             13297..13494
                     /locus_tag="ACG8KQ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104615.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13647..14132
                     /locus_tag="ACG8KQ_001683"
     CDS             13647..14132
                     /locus_tag="ACG8KQ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9104616.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14236..14829)
                     /gene="clpP"
                     /locus_tag="ACG8KQ_001684"
     CDS             complement(14236..14829)
                     /gene="clpP"
                     /locus_tag="ACG8KQ_001684"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9104617.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15009..15536
                     /locus_tag="ACG8KQ_001685"
     CDS             15009..15536
                     /locus_tag="ACG8KQ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104618.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15627..16364
                     /locus_tag="ACG8KQ_001686"
     CDS             15627..16364
                     /locus_tag="ACG8KQ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9104619.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16368..17285
                     /gene="miaA"
                     /locus_tag="ACG8KQ_001687"
     CDS             16368..17285
                     /gene="miaA"
                     /locus_tag="ACG8KQ_001687"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9104620.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17287..18516
                     /gene="hflX"
                     /locus_tag="ACG8KQ_001688"
     CDS             17287..18516
                     /gene="hflX"
                     /locus_tag="ACG8KQ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9104621.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5991 a         3205 c         4028 g         5308 t
ORIGIN      
        1 gcagatgcaa gcttttgaac accgttttat ttgtctcaca cccttttcct tcctacttgt
       61 aaccttgctc ccgtgttaga aacatgttac actaaatatg tgaaattatt aacaaggagc
      121 gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa ctgcgaaaag attaccttta
      181 tatttacgct acttgaagat gctggatgac tcaggtataa gtagaatcaa atcaaatgag
      241 ttcagcgaaa tcacccaaat tccttctgca acgatccgca gagacttctc gcaattaggc
      301 gagctaggca gaagcggata tggctatgat gtcccttttc ttatcgatgt attcaataat
      361 atccttaata ctaaagaaga aaaacgaatc gcattagtag gatatggaaa tcttggcaaa
      421 gcattgaaac acaacaattt tagaagaaat gaaaatctaa atatcgtgtg cgtctttgat
      481 aatgatccag cactgatcaa tcgagtaatt gatggagaaa tgatctatcc gattgatcgt
      541 ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa tctctacggt accaagtaaa
      601 tattcacaat ctgctattga tgaaattgtc aaaggaaatg taaccgctat acttaatttt
      661 gcgcctgaca gagtaactgt cccagcttat gtcaatgttc aatacattga tttgacaacc
      721 gagttgcaga cgcttattta ctttgatgag aattactcag aagttttttc ttaggaaaat
      781 ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt ctttcaaaaa ttttttagta
      841 ctaaatgaag tggttttttc acgaccactt gttgactaaa aaaaaaaacc attgtagaat
      901 gaagcataac gctgaacaag aggagtaact ggcacattca ttcttagaga gagaactatc
      961 tgctgaaagg ttctcaatga caaccagtga aggtagcttg ggagtttctg tcttgaatgg
     1021 agtagagata gacgagtcgt gatcgttaac tcacttgaga ggcaataatg attgattatt
     1081 gcaatttagg tggtaccgcg taaatcatac gtcctagaat acgaaagtat tttaggacgt
     1141 tttttagttt aaaggaggaa aacagcatga cggaagaaaa taacctatca acaaaatata
     1201 atccgcaaga agttgaagct ggacgttatc agcattggtt agaacaagat ttgtttaaac
     1261 cgagcggaga taaaaaagca aaaccttatt caatcgtaat cccgccccca aatgttactg
     1321 gaaaacttca tttagggcat gcttgggata cgaccttgca agatatgctg attcgtcaaa
     1381 agagaatgca aggatatgat actttatggc tgcctggtat ggaccatgca ggaatcgcga
     1441 cacaagcaaa agtagaagcg aagcttagag agcaagggat ttctcgttat gatctaggtc
     1501 gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga gtatgcgagc cacatccgcg
     1561 aacagtgggc taaaatgggc ttatctttag attacagtag ggaacgtttt acattagatg
     1621 atggattatc tgaagcagta cgtaaagttt ttgttacctt atacgaaaaa ggtttgattt
     1681 atcgtggtga atatatcatc aactgggatc ctcaagcacg aacagctcta tctgatatcg
     1741 aagttattca taaagatatc gaaggcgcct tttatcatat gagctatgaa ctagcagatg
     1801 gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac gatgttggga gatacggcga
     1861 ttgctgttca tccagaagac gagcgttatc aagcgctcat cggcaaaaaa gtgattctac
     1921 ctttagtcaa taaacaaatc ccaatcattg cagatgaata tgtagatatg gaattcggga
     1981 caggtgtagt aaaaatcact ccagcacatg atccaaacga ctttgaggta ggtaatcgtc
     2041 atgatcttcc acgcgtaaat gttatggatg atgatggtac gatgaatgaa ctagcaggaa
     2101 aatatgctgg aatggaacgt tttgctgctc gtaaagcaat cgtgcaagat ctgaaagaaa
     2161 tcggtcgatt gattaaaatc gatacgatga tccacagtgt gggtcattcc gagcggacag
     2221 gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt aaaaatggcg ccgttagcga
     2281 aaaaagccat tgataaccaa gaaacagatc aagcagtgga attcttccct ccaagattca
     2341 atcaaacgtt tttacgttgg atggaaaatg tccatgattg ggtaatctca cgacaattat
     2401 ggtggggaca tcaaattcct gcatggtatc acaaagaaac aggtgaaatg tatgtaggga
     2461 tggaagcacc agcggatagt gaaaactggg tacaagatcc tgatgtttta gacacttggt
     2521 ttagttcagc tttgtggcct ttctcaacaa tgggctggcc agatacggaa agcgaagatt
     2581 ataagcgata cttccctact agcactttag taaccggtta tgacatcatt ttcttctggg
     2641 taagcagaat gattttccaa agcttggaat ttactggcga acgtccattt gagaatgtct
     2701 tgattcatgg attgattcgt gacgaacaag ggcggaagat gagtaaatca ttaggtaatg
     2761 gaatcgatcc gatggaagta atcgaaaagt atggagcaga tgctctccgc tggttcttat
     2821 cgaatggttc tgcaccagga caagatgtcc gcttcagcta tgaaaaaatg gatgcttctt
     2881 ggaactttat caacaaaatc tggaatgctt cacgtttcgt tatcatgaat atcgaaggaa
     2941 tgaccgtaga agagatcgat ttatctggag aaaaatcagt ggctgaccgg tggatcctta
     3001 ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt tgatcgtttt gaatttggtg
     3061 aagcaggtcg acaactgtat aactttatct gggatgattt ttgtgactgg tacatcgaaa
     3121 tgagtaaaga agtactttat ggagatgatg ctgtttcaaa acaaaccacc cgtagcatct
     3181 tagtccatac acttgatcag atcctacgtt tattgcatcc aatcatgcca ttcgttacag
     3241 aagaaatatg ggaacatatt cctcatcagg gaaattctct agtagtggca gaatatcctg
     3301 ttgtacgtcc agagtttgat gatgaaacag cttctaaagg gatggaagta ttgaaagaat
     3361 tgattcgagc cgttcgaaat attcgttcag aagtgaatac accattgtct aaaccaatta
     3421 cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt tttagttgaa aatacaagct
     3481 atatcgaacg cttctgtaat cccgaagagt tgacgatatc aagtgagatc actgcgccag
     3541 atctagcgat gtcagctgtt ctgacaggtg cagaaatctt cttgccgtta gcaggattga
     3601 tcaatattga agaagaaatc aaacgattag aaaaagaact ggaaaagtgg acaagcgaag
     3661 taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt ttctaacgcg ccggaagaag
     3721 tagtagaggc agagcgtgca aaagaaaaag attatttaga gaagcaaacg gctgtcaaag
     3781 aacgaatcga aagtttgcgt actattcaat aagaatagaa aaagagagtg tgaaatcagc
     3841 tagctattac taaaagctag ctgattgaac atcgttacag gaaagagttt gagcctcact
     3901 cttagcttcg attcataagg agatgtgaca taaattttgt cgcatctctt ttttagtcta
     3961 ctcttattat gtaggagaag gagggaggtg gatcatgtat caagagtcaa taaagtagtt
     4021 gataaaatcg ttcctctctc tgtttctttt ctctttgtca tacttttatt tttcttatgt
     4081 gttctatttg ttataataaa agagaaatgg gggaattgtt gatgacgatt gaagaagcaa
     4141 tagaatggat ccatagtcga ttaccatttg gttcacgtcc tggccttgac cgagtcgaag
     4201 ctttattaga agtggtaggg catccagaga gaaaagtaaa aacgatccat atcgctggaa
     4261 caaacggaaa aggttctact gtagcttatt tgagaagcct attagaagag gctgggctga
     4321 cagtaggtac atttacttca ccttatatcg aaacgttcaa tgaacggatc gcaatcaatg
     4381 gcaaaagcat tccagacaat gatttgatcc agcttgtaaa aaaatatcag ccactgatca
     4441 aacaacttga tcaatttgaa gcagtagctg gtattactga attcgagaca ttaacagcaa
     4501 tggcatttga ttattttttg atgaagaaag tggacgtagc gattatcgaa gttggcttag
     4561 gcggcttatt ggattctacc aatgtagctg taccaatgct gacgggaata acaacgattg
     4621 gacttgatca taccgaaata cttggagata caattgaaga aatcgctgca caaaaagctg
     4681 gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat tgccccagaa gcttttactg
     4741 tcatccaaca aacagctaaa gcaaaaaatg caccgcttta tgtatacggg gaaaattata
     4801 aaataaacta tctgcatcct gatgagatgt ggggagaagt atttgaattt tttaatgaaa
     4861 taggaaagat ccctcaatta accactccgc ttttagggca acatcaagct gaaaatgcag
     4921 gagtagccat cgaattgtat tttgtttact gtcaattgga aaagctgcct ttccgagaaa
     4981 aagatgtttt gaagggattg aagaaatctc gatggccagg aagaatggag cgtttgagta
     5041 acgagccatt agttgtttta gatggtgccc ataatacgca tgcaatgaac cggcttgttg
     5101 agaatttgaa aaaagaattc aaaggatatc aaatcaacat tttgttttcc gccttacgaa
     5161 ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt accaaatgtc catatttatt
     5221 tgacgacctt tgaacatcca aaagccatcg aattaagtga agtatcagat ttagcaagtg
     5281 agagagtatc aatcgtttca ttgtggcaat tcggattagc agatatattg gaaaagatga
     5341 caaatgaaga cgtattagtt gtgaccggtt cactttattt catttcagaa gttcgccatt
     5401 tattaataca attaggagga gatcatgaag tataatgcag tgatttttga catggatggt
     5461 ttgctgttcg acacagagtt agtgtattac gaagccacac aagtagtagc agacaagatg
     5521 ggttttcctt atgataaaga cctgtattta aaattcttag gtgtttcaga tgaagaagta
     5581 tgggcgaatt atcatatgat tttctcggaa tttggcaaaa caagtgtaca aagattcata
     5641 gatgattcat accaggaaac gcttgaacgt ttttcgcaag gagcggtaca aatgaagccc
     5701 ggtgtgttgg aactcctctc ttttttagaa gaacatcaga ttcccaaagt tgtcgcttcc
     5761 agtaaccaac gagcagtcat tgaactttta ttgaagaaga acaatctgtt tgaacgattt
     5821 gagacgattg tttctgcaga aaatgttcaa cgagcaaaac cagacccaga aattttcttg
     5881 ttagctaatc agaagctagg tacagcgaag aaagaaacat tgattttaga agattcacaa
     5941 aatggtattt tagcagcttc tgcggctgag atcccggtag tcatggtacc agatctgctt
     6001 ccaccatccc aagaattgat caaaaaaaca gctggggttg tttcttcttt acatgaaatc
     6061 cctgcattgt taaaataaaa atccatctct tttcgtatct tattttagat aagatagaaa
     6121 ggagatgttt ttttgggaaa acgattgatc aaagaagtac ctactagctc tttaccaagg
     6181 gaacgtatgg aaatttatgg cgcggaagct ttatcggatc aggagctttt ggcaatctta
     6241 ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg ctggaaatct gttaaaaacg
     6301 tttggaggac tggcctcttt acgacaggct actttgcatg agttagaaga gattcgaggg
     6361 ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg aattaggtcg acgaattcag
     6421 acggatcatg aaagagcggc tccaccggtt cgtaccagct acgaattggc ccagcaattg
     6481 attctggaaa tgaaagatca caaacaagaa catttggttt gtatttattt ggacacgaag
     6541 aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc tgaatcagtc agtcgctcat
     6601 cctagagaaa ttttccatta tgctgtacgt tattgtgcag caaggatcgt tttagcgcat
     6661 aatcatcctt ctggtaatgt tattccatcg cagcaggata tgaattttac caaaaggata
     6721 caaaagtgtg gagaaatgat ggggatcact gtactggacc acttgatcat cggacgcaaa
     6781 cgatatttta gtttgagaga agaaggaatg atggaagaga agtaagttgc tttcttgcaa
     6841 aaaaaatgaa gcaagtgtac aattatattg taattttgtt tgggaataga agttgaaaca
     6901 tctgttgcac ctctatcgta cacgagcaat gttacaattt aaattagtgc ttttggcacg
     6961 aggaggatat tattatggaa aacggaacag taaaatggtt caacgctgaa aaaggatttg
     7021 gatttatctc acgtgaagat ggaagcgacg tattcgttca cttctcagct atccaaggtg
     7081 acggattcaa aacattagaa gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc
     7141 gcggcccaca agctgcaaac gttgtaaaag cttaatttta tctgtaaatc aaattgatgg
     7201 tagacatcaa tttttacgag agaacaaagc gagtaacaaa aatacctgcc acaagagaca
     7261 atttctcttg tggcaggtat ttttttgtat gaaaaagtaa ataaatttaa gaggctatga
     7321 aagagccacc ttgtatcaag gaataagaac aaacaaaaat ggcttctcat gtttttcatt
     7381 tgttttctct tatattcagg acaaggtgct gatttcataa cctcttattt attcttcatc
     7441 tggtgtcatt accattggca aaatcattgg atgtctttct gtacgttcga ataagaaagg
     7501 ctgaagcgcg ccagtcatcg cttctttcaa tttatattct gaacagtcag gtccattcaa
     7561 acattctctt aaagcgtgga ataagattcg ctgagcttca ttgatcattt ctcctgattc
     7621 tcgcatgtag acaaatccac gagagagaat atctggacca gccaagattt cttttttctt
     7681 gatatccact gtcgcgacag ctaatacaag accttcttct gaaagcaggt gacggtcacg
     7741 taaaacgaca ttaccaatat caccgatccc gctaccatcg atatacacat ctccagccac
     7801 aaaatgccca gcaggacgcg cagaatcgct agttaacgct aaaacatcgc cattttccat
     7861 gatgaagcag ttttctgcag gaacacctac atcttgtgct agagaagcat gtactttcaa
     7921 catgcggaat tctccgtgga caggaataaa atattttggc ttcatcaaac gaagcattaa
     7981 tttttgttct tcttgtccac cgtgaccgga agtatggatg ttattgattt ttccgtgaat
     8041 cacttctgca ccagcttcag acagcaaatt gatcaaatga ttgacacttg ttgtattacc
     8101 aggaataggt gaacttgaaa agatcactgt atcgtctggc tggatttgga tttgacggtg
     8161 agtaccattc gcgatgcggc tcaacgcggc catcgcttct ccttgagaac ctgtacatag
     8221 gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc
     8281 tggaactttg atatatccta aacgttgacc gttgacgatg gcattttcca tgcttcgtcc
     8341 gaaaacagcg attttacgac cagttgccac agcggcatct gctgcttgct gcaaacggaa
     8401 gatgttagaa gcaaagctgg caaaaatgat acgtccttcg atttttgaaa agatcttcaa
     8461 aatagaatgc ccaatcgttt tttctgattt agtaaatgtt ggaatctcag catttgtact
     8521 atcagataaa aggcacaaga ctccttcttc cccgatacga gccattttat gcaagtttgc
     8581 tggttcgcca acaggagtga aatcgaattt gaagtctcca gtcgctacga tatttccaga
     8641 aggtgttttc accacgatcc ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct
     8701 gacagaagtc tttctaaaac ggatgatcgt atcttcattg atctcatgga gctctgcatc
     8761 tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc
     8821 atagatagga atatttgctt gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg
     8881 cccgtgagta atcaccaagg cttttacttt gtgtaagttt tgtatgatgt agctgtagtc
     8941 gggaatgaca tagtcaattc caagtaaatc gtcttccggg aatttgatcc cggcatcaat
     9001 caaaatgatt tcatcttgga attgtacccc atacgtattt ttcccgattt cacctaatcc
     9061 accaatagca aagacgcctg tctcgttatt tttgatggaa agtttcatat taaaactcca
     9121 ctaatgagaa atcagcattt tctttttcgt actctagatg acttccttct agaagttgaa
     9181 cgaattcaat attgtatggg gtattttctt ccacacgttg gcgagcgtct acatcgcttt
     9241 ttgcctcaat gtaaagagat tttgtttctt cccgtttagg gttgcgtact tttgtttctt
     9301 gataataaac tttatagatc attgttttgt ctcctttatc tgcgtaaagc agtgatttag
     9361 ctagttgatt tttttagaca acacaaaacg aggggacaag ccacctggtt tttcatatgc
     9421 tttgactaga atactggatt cgagtcaaag agacaagcct cgtttttgct tatctgttcg
     9481 gatatttttt caaccgtacc ttctgtataa aaaatataca acaattaatt gttattctag
     9541 cataaaacag caaataagta tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc
     9601 atcccgctat aatcataatg acttttttat gagagaagtg ttctattaat aggctcgttg
     9661 tgctatcatt tttagtaatg aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa
     9721 ctaatgtggc attataccat gcgttataag aaattcttgt ttttcaattt catatgcgta
     9781 tttggtttta ttttgattga attaggactt cctacgattt tagcacgtat gatcgatgta
     9841 ggaatccgca atgatgattt tgattatgtg aagcaacaag ggatatttat gatcgttatt
     9901 actgtaatcg gtattatttt gaatatcttg ttaggttatt ttggttcgag gattacgact
     9961 aatatcgtag cagatattcg agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag
    10021 gaatacgaaa atctaggcgt atcttcattg atcactcgta caacaaacga tgcttatcag
    10081 atcatgttgt tcctgcaaaa tattttacgt atcggtttta tggcaccaat gatgttcatt
    10141 gtcagtttgt acatggtcat gcgtacaagt gtgacactca gtttgtatgt agtaggtgca
    10201 ttacctttac tattattggc agtcgtagcg attgctaagg tgtctgaacc gttatctaaa
    10261 aagcaacaaa aaaacttaga taaaatcaac agtatcttgc gggaaaatct ttcaggattg
    10321 cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat
    10381 gagaattatg cttctagttc aaaaagctta ttccgtttga tggctgttgc acaaccgggt
    10441 ttttatttct tatttaatat cgtaatggta ttgatcatct ggagcggggc agtgcaaatc
    10501 gatgcaggcg agcttcgtgt aggagatttg attgcgttta tcgagtatat attccatgcc
    10561 ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt atccaagagc agctgtttca
    10621 gctggacgta tccaagaagc cctagatgcc aaaccttcga ttgtagaaga tccaaatggt
    10681 atatctgata caaaaactaa agggtatatc gaatttcaaa atgtaacatt tgcttatcca
    10741 ggccattcgc aagagccagt gatacgaaat gtcagtttta cagcctctcc aggagagacc
    10801 gttgctttta ttggaagtac cggtagcgga aaatctacgt tgatccaatt gatccctcgc
    10861 ttttatgatg tgagtgaagg aaagatcttg atcgatggag tagacgtgag agattatcgg
    10921 ctaagcaagt tgcgagataa gattgggttt attccgcaaa aagcgctgct atttactgga
    10981 acgattgcag ataatttgcg atacgggaaa gaagatgcta cgcaagaaga aatggaacgt
    11041 gcagctgaga ttgctcaagc ttcagaattc atttctcgaa aaccagatgg atttaatgaa
    11101 catttatcag aaggtggttc caatttttca ggtggacaaa aacaacgatt ggcaatagcc
    11161 agagccatca tacgccgtcc ggaaatctat atttttgatg acagcttttc tgcattagat
    11221 tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag aaacgacaga atctactgta
    11281 ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag ataagatcat cgtattaaat
    11341 gaaggagaag tcgtcggtat ggggacgcat cgggagttgt tggaaaaatg cccgatttat
    11401 tatgatattg ccgcttctca attatcaaag gaggaattag catgaaaaaa accttttctt
    11461 ccttaaaacg tttagctcga tatatcagac cgtatcgtga gacttttctt ttagtgatcc
    11521 tatttacagt tctgacagtg gctttcaacg tggctttgcc atatgttaca ggacttccta
    11581 ctacagaaat cagccgtaat attgccaatg gcgaatcaat caatttttct tatatattca
    11641 aatgtctcat ttggatcaca gttgttggaa tagggtattg tatttcgcaa ttactttctg
    11701 gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga tttgagaaga gacatagaag
    11761 aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa ccagcaagga aatattttgt
    11821 ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt acaacaagct tttattggta
    11881 tcgtaaatgc gatattggga attacgttgg ctgcagcaat gatgttttat atccagccga
    11941 tgatggcgct gatctctatg atcatgattc caacatcgat ctggatctca aaacgagtga
    12001 tcaatgcttc gcagaaatat tttcaaagca tgcagaattc tcttggtgag ctaaacggct
    12061 acgtccaaga aaatatgaca ggttttagtg tattgaaagt atatggacgc gaaaaagaaa
    12121 cattggaagg attcaaaaaa gttaatcaca gcttgaaata ctttgggttt cgtgcagcat
    12181 tcatttctgg cttgatgatg cctctagttc aattaactgc ctatgctact tatattggta
    12241 tggctgttct aggaagcttt tatgttatta ctggggcgat cgttgtcggt caactacaag
    12301 cattcatcca atatatttgg caaatcagtc agccaatggg gaacgtgaca caattgtctt
    12361 cagttttgca aagtgcatca gcggcaacga aacgtgtatt cgagatttta gatgagccag
    12421 aagaaaaaga aaacaaagta gatgcttcac tgccagaaac gattttagga gatgtgacat
    12481 tcgatcatgt cgattttgca tatgatccga agaaaccatt aatccaagat ttgaattttg
    12541 aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac cggtgccggc aagacgacat
    12601 tgatcaatct attgatgcgt ttttatgacg tagacaaagg agcaataagg attgatggaa
    12661 tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag
    12721 atgcatggct ttatcaagga acgattgcag ataatatccg cttcggaaag cttgatgcga
    12781 cagattatga agtagttgat gctgcgaaaa cagcaaacgt agatcatttt atccgaacga
    12841 tgccagatgg atatgatatg gccatcaatt ctgagggaga caatgtgtct ttaggacaaa
    12901 aacagttgct gacgattgcc cgagcagtgg tctccgatcc taaaatcttg attttagatg
    12961 aagcaacaag ttcagtagac acccgtttag aagcgttgat tcaaaaagca atggatcgcg
    13021 tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag
    13081 atctgattct agtgatggat caaggtcaga tcattgaaaa aggaacgcat gagagtttat
    13141 tggcacaagg tggattttat gaaaaactgt ataacagcca atttgctgag gaagcagaat
    13201 aagtcatata agaataaaac agtttaaaac ttattttttg ttctctattc ttgataatgg
    13261 taaagtaaag ttatcaagaa aagtgaggac acggagatgg aacagttttt aaatgaaaaa
    13321 attactgaag ccaaaagaga agttgatcgt ttaacgcacg tctgtcagac gaagcctttt
    13381 gattcagctg atttttttga aaatgagttt caaatcgaac gcttggcagc acaaatagaa
    13441 gtgtacgaat taattttaaa aaaagtaata gaagaaaata tagatgtaca ataaaaacag
    13501 gtaaaacgtc agttaccaga tactgtaaat agacatctgg cctgagcagg ttttacctgt
    13561 tttttttctt atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta
    13621 caaaaaatga aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga
    13681 ttggtgatgt gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg
    13741 acagtaattg gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg
    13801 tcgatcatga tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc
    13861 tacatggctg tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc
    13921 atgcggttat tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag
    13981 ttattcctcc taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa
    14041 aagaagaaat acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc
    14101 atgcgcaagg aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga
    14161 tggtcagttt gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc
    14221 ttcctttgaa acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc
    14281 atatgctttt gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac
    14341 ttcaagcggt tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa
    14401 aatatggcgt gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg
    14461 atggatcatg atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag
    14521 caagaagctt cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac
    14581 aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat
    14641 ataaagatag atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat
    14701 cgaattagca agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa
    14761 acgtgagtag atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat
    14821 taaattcata atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt
    14881 tctaatacct aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg
    14941 caaattagtc gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga
    15001 acaacagatt gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg
    15061 tctatatcgg catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg
    15121 aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg
    15181 agatcttgca aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg
    15241 attgttatat cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt
    15301 tttatctgcc aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga
    15361 ttttatctcc agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga
    15421 ttttagagaa ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga
    15481 agactatgac gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt
    15541 ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac
    15601 aatagaactg aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg
    15661 gcacccaccc agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg
    15721 gtatcgaact agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag
    15781 aagtggaccg gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca
    15841 aaaaattgaa tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat
    15901 tgagagaagt gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa
    15961 ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa
    16021 gtagagattg gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa
    16081 tcgcattact ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga
    16141 tccaactagc agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt
    16201 tcgaacatcc agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg
    16261 atgaagaaga aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc
    16321 cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat
    16381 tagtgatcgt cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac
    16441 tatttggagg agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg
    16501 gtacagcaaa aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga
    16561 tcgaacctac tcaacgttac tcagccgttg attttcagca ttcaggtcga caattgatta
    16621 ccgagattgc gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc
    16681 aatcccttct atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa
    16741 aaaagtatga aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag
    16801 aaaaagaccc ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag
    16861 ctctggaagt tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc
    16921 aattgtatga ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa
    16981 tcaatcaaag agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt
    17041 atgagcttgg agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt
    17101 ttgaagggaa agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat
    17161 acgcgaaacg tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta
    17221 taagtgagcc aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg
    17281 aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac
    17341 ttttcagagt caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta
    17401 ttcacaatta cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa
    17461 ttacaagaat tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat
    17521 gagctaactc cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat
    17581 cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg
    17641 caggttgaac ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca
    17701 ttatctcgtc ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca
    17761 gaccgccgac atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa
    17821 gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt
    17881 attggctaca cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact
    17941 tattcgaaag atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct
    18001 gaaggatttg agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa
    18061 ctgatcgatg ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat
    18121 gtagtcgatg ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg
    18181 gatgaactga atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc
    18241 gacccagcga tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa
    18301 gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct
    18361 tatacacttt ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca
    18421 atggttttgg acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca
    18481 aaaaccaatt caaaatggtt aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DAZKCE010000055        18517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000055 DAZKCE010000000
VERSION     DAZKCE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.055"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..346)
                     /locus_tag="ACG8KQ_001689"
     CDS             complement(<1..346)
                     /locus_tag="ACG8KQ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104622.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSP"
     gene            613..2313
                     /locus_tag="ACG8KQ_001690"
     CDS             613..2313
                     /locus_tag="ACG8KQ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9104623.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2283..2501
                     /locus_tag="ACG8KQ_001691"
     CDS             2283..2501
                     /locus_tag="ACG8KQ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9104624.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2521..3411
                     /locus_tag="ACG8KQ_001692"
     CDS             2521..3411
                     /locus_tag="ACG8KQ_001692"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9104625.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3429..3713
                     /locus_tag="ACG8KQ_001693"
     CDS             3429..3713
                     /locus_tag="ACG8KQ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104626.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4200..4814
                     /gene="gmk"
                     /locus_tag="ACG8KQ_001694"
     CDS             4200..4814
                     /gene="gmk"
                     /locus_tag="ACG8KQ_001694"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9104627.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4819..5124
                     /gene="rpoZ"
                     /locus_tag="ACG8KQ_001695"
     CDS             4819..5124
                     /gene="rpoZ"
                     /locus_tag="ACG8KQ_001695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9104628.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5377..7785
                     /gene="priA"
                     /locus_tag="ACG8KQ_001696"
     CDS             5377..7785
                     /gene="priA"
                     /locus_tag="ACG8KQ_001696"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9104629.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7800..8291
                     /gene="def"
                     /locus_tag="ACG8KQ_001697"
     CDS             7800..8291
                     /gene="def"
                     /locus_tag="ACG8KQ_001697"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9104630.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8284..9222
                     /gene="fmt"
                     /locus_tag="ACG8KQ_001698"
     CDS             8284..9222
                     /gene="fmt"
                     /locus_tag="ACG8KQ_001698"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9104631.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9222..10580
                     /gene="rsmB"
                     /locus_tag="ACG8KQ_001699"
     CDS             9222..10580
                     /gene="rsmB"
                     /locus_tag="ACG8KQ_001699"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9104632.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10593..11333
                     /locus_tag="ACG8KQ_001700"
     CDS             10593..11333
                     /locus_tag="ACG8KQ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9104633.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11330..13399
                     /gene="pknB"
                     /locus_tag="ACG8KQ_001701"
     CDS             11330..13399
                     /gene="pknB"
                     /locus_tag="ACG8KQ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9104634.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13564..14463
                     /gene="rsgA"
                     /locus_tag="ACG8KQ_001702"
     CDS             13564..14463
                     /gene="rsgA"
                     /locus_tag="ACG8KQ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9104635.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14476..15126
                     /gene="rpe"
                     /locus_tag="ACG8KQ_001703"
     CDS             14476..15126
                     /gene="rpe"
                     /locus_tag="ACG8KQ_001703"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9104636.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15127..15765
                     /locus_tag="ACG8KQ_001704"
     CDS             15127..15765
                     /locus_tag="ACG8KQ_001704"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9104637.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15920..16774
                     /gene="mreC"
                     /locus_tag="ACG8KQ_001705"
     CDS             15920..16774
                     /gene="mreC"
                     /locus_tag="ACG8KQ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9104638.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16778..17287
                     /gene="mreD"
                     /locus_tag="ACG8KQ_001706"
     CDS             16778..17287
                     /gene="mreD"
                     /locus_tag="ACG8KQ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9104639.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17542..>18517
                     /locus_tag="ACG8KQ_001707"
     CDS             17542..>18517
                     /locus_tag="ACG8KQ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9104640.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         6274 a         3359 c         3977 g         4907 t
ORIGIN      
        1 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
       61 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
      121 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
      181 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
      241 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
      301 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
      361 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
      421 ttttaaaatt ccattacaaa atcacgtttt cattgaaaac actctcattt tcaattaatt
      481 tttgtgattt ttgtattttt ttatttaaag gaggttttat gaattgtttt cttatggaat
      541 ctacaaagtc gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa
      601 aaggagagcg cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg
      661 cctatccagc aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac
      721 gctttttggc gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc
      781 gtctatttta ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa
      841 ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt
      901 attaaagaaa actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt
      961 tttcataaaa acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca
     1021 gcacttgttt taggtggagg aggagcgcac ggtgcttatc agattggtgt gtggcaggcg
     1081 ctgaaggaac atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac
     1141 ggcgcattga ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg
     1201 acaagacaag tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc
     1261 tatcgagaaa gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct
     1321 ccattggaaa agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa
     1381 atacgtttat atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc
     1441 caacagacaa aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc
     1501 gctatggctt atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa
     1561 atacctattg atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa
     1621 gggccaggcc cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa
     1681 acattgtgga cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta
     1741 caattaggct atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca
     1801 tttcattctg ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa
     1861 gaaattcagt tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat
     1921 ttatataaga acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca
     1981 aaaaaacatt ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc
     2041 tgccaacaag atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt
     2101 actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa
     2161 actgtttatt ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg
     2221 cttaagcaac cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa
     2281 ccatggcaca agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca
     2341 acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg
     2401 atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg
     2461 aagagtttga acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga
     2521 atgaaaagta tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat
     2581 attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc
     2641 ctgaactttg ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga
     2701 atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac
     2761 tgggaattga ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt
     2821 agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct tcgtgctgga cgaaaacttc
     2881 gtaacggtag aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat
     2941 acattgaaaa gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta
     3001 gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt
     3061 tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt
     3121 cttggagcga aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc
     3181 ggagatattc acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt
     3241 atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg
     3301 gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag
     3361 tctaagacga tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg
     3421 tagagataat gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta
     3481 aacgggatat cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact
     3541 ataatgagaa cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga
     3601 aatttattgt tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc
     3661 gaaaagatga gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat
     3721 gctgaacaaa gaaatacaat aaataaacag aattggattt cttcgcttag cttctttgct
     3781 ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat
     3841 aagtgatgtt caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa
     3901 actattttca caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta
     3961 tacacggcgt tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag
     4021 aagctgttta tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct
     4081 tggtacaggg tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg
     4141 cagaaaaaaa gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca
     4201 tgtcagagcg tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag
     4261 tacgaaaagc aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta
     4321 cccgtaaaat gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag
     4381 agtttgaatc aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt
     4441 attatggaac tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct
     4501 tggagattga agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca
     4561 tctttttgac tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag
     4621 atgcagatga agtaatcgaa aaacgaatga aagttgcaaa agaagaaatc gaaatgatgg
     4681 ctctttacga ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa
     4741 acattattgc cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt
     4801 taaaggagat gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta
     4861 aattcaaaat attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt
     4921 gcacagccta ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa
     4981 atcgatgcag gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag
     5041 atggaagaag aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt
     5101 atccgtgaag agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc
     5161 aaacccagct gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt
     5221 ctgaactttt tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt
     5281 ggcagaagat aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc
     5341 ttgataagaa aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt
     5401 gtggacgtac ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg
     5461 gaaacagctt tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat
     5521 gtccaaggat ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag
     5581 gcagttattc gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct
     5641 gattatatga aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg
     5701 agtgtgatga aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt
     5761 caggtattgt ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc
     5821 ctttctcaat tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat
     5881 acacgaaata aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc
     5941 gaagaagaat gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt
     6001 ttatctgaac tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact
     6061 gctgtcttga atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat
     6121 cgcgaccctt ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa
     6181 caaaaaaatg ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt
     6241 ttgctggagg gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat
     6301 gttttgtcag aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa
     6361 atggtcgagc gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt
     6421 tcccaaggtg aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta
     6481 gtaggcgcac gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat
     6541 gaagaacatg aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta
     6601 gcaatttggc gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct
     6661 ttagaatcaa gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga
     6721 gccaaagcag ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag
     6781 aatcatcgga cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac
     6841 aaaaaagaac aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt
     6901 agggactgtg gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg
     6961 gatacacgtt caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt
     7021 cctaattgtg gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag
     7081 gaactccgag aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga
     7141 agaaaagggg cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta
     7201 ttagggacac aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg
     7261 ttgaatgctg atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa
     7321 ttgcttaccc aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc
     7381 caaacattca atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag
     7441 ttttatcagc aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact
     7501 gtgaaaataa ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt
     7561 gccaatcagc taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc
     7621 atgattttga gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa
     7681 ccgaatcttc ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa
     7741 ggtctttttg tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta
     7801 tgcgctatcc tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg
     7861 atgtaatcac ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga
     7921 atgacggaat agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg
     7981 aagtagatga aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg
     8041 aaagccttga cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag
     8101 cagatgaagt gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt
     8161 ttggctattt agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca
     8221 ttgagaaaat gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc
     8281 ttgatgacta agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg
     8341 ctgatagaat caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg
     8401 cggaaacgag taattacgcc gacaccggta aaaaaagcgg ctctgaagca cggtatccgt
     8461 gtgctgcagc cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg
     8521 cctgatgtga tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta
     8581 ccaaaattag gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg
     8641 cctgttcatt atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg
     8701 atcaaaaaaa tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa
     8761 gatgatgtag ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa
     8821 acattgccca atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact
     8881 ttttcgccaa acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa
     8941 attgacaacc aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc
     9001 atacgctgga aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt
     9061 acgatcatcc ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta
     9121 gccatcaatg aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac
     9181 ggaagcggtc agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa
     9241 aaatgaaaaa atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg
     9301 gcgcctactc gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag
     9361 atgtgggttt atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat
     9421 tttacgttgc accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagatgcttc
     9481 tttatctttc tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa
     9541 acgaggcggt cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg
     9601 gtgtcttgag gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta
     9661 tcgaaagact ttctgtcaaa atcagcctgc ctctatggat gacgaaacga ctggtggatc
     9721 aaatcggata cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta
     9781 gtgcccgagt agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag
     9841 aagagatcac agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc
     9901 atttagcttc tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga
     9961 tgcttgtagc gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag
    10021 cacccggagg taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag
    10081 tggtcgcttt agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc
    10141 taggtgtctc ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt
    10201 tcgaagcaga gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc
    10261 ttcggcgaaa accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa
    10321 aaatccaatt ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga
    10381 cttatagcac ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg
    10441 aaagacaccc tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa
    10501 tcgaagagaa aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt
    10561 gttgtttgcg caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag
    10621 gaagacgacg taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca
    10681 agcttgctgt attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga
    10741 tggcggtaac gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa
    10801 tcgcccaatg gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac
    10861 aagaacagcc ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg
    10921 actggttcac gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa
    10981 tcatgcaatt gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca
    11041 gcgaagaaat ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc
    11101 ctggaacagt cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt
    11161 tatgttcaga tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc
    11221 aagaaggccc attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg
    11281 gagcggataa tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt
    11341 gggcaaaaaa ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc
    11401 aaatgtattt ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg
    11461 atttgacttt caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc
    11521 aacagaactc gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat
    11581 gcagtatctg gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca
    11641 ttatcctgtt ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc
    11701 tttagctcat aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga
    11761 taatgaaggt gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc
    11821 gatcacgcag acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag
    11881 aggaagtatg gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat
    11941 gttgacgggt agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt
    12001 tcaagatgat cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa
    12061 cgtgatttta cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat
    12121 gtctgatgat ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc
    12181 tcatgtgatg gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc
    12241 tgattcattc aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga
    12301 ggaggaagaa gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct
    12361 attagctatt cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg
    12421 aggagaagtt gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact
    12481 cgcaaaagcg aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga
    12541 agaaggtaat gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga
    12601 agtcactctg tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa
    12661 aagttatgaa gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca
    12721 aaaagaagag gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc
    12781 agaagataca gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc
    12841 agaaacagtg actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct
    12901 tgttacacaa ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt
    12961 accatcaggt tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac
    13021 ttacatcaat tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta
    13081 tacaaaaagc gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga
    13141 gacttccgaa tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc
    13201 aggaacgaca attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc
    13261 agcgtcttct agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag
    13321 tagcacaagc aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac
    13381 ctcagaaagt tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgatt
    13441 tgttgcattc tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt
    13501 gagaagtttg catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg
    13561 gaggtggcac ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac
    13621 gcaaacggag agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg
    13681 ccgcttgtcg gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg
    13741 gaaatcttgc cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga
    13801 atcattgtca caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta
    13861 gttaccttgg aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca
    13921 gacgacccag aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg
    13981 attgtaccca atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta
    14041 tcggtcttta tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg
    14101 gaactgaatt tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca
    14161 cgtcatgtgg aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc
    14221 tcgattgact ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta
    14281 gaagcttctg tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa
    14341 gtaaaacata gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa
    14401 ttcttacaag aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa
    14461 taggaggaaa caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta
    14521 caaagagaca ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg
    14581 gatggccgat ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct
    14641 gttactaaat taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca
    14701 gcatttgcaa aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt
    14761 catcgggcac ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca
    14821 ggaacaccag tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg
    14881 actgtcaatc ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg
    14941 gaactcgctg atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga
    15001 atcgttccag aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga
    15061 tcatatatct atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta
    15121 gactaaatga aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg
    15181 ctgtccaaat acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca
    15241 ggctacccag tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag
    15301 gaagtatttg caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat
    15361 acacagttag gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt
    15421 ggagctacgg gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct
    15481 cgcttcaaac cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat
    15541 tatttgcctg gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc
    15601 tgcttgactc ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa
    15661 gaagtagaat atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt
    15721 tctttttcag atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa
    15781 aaaaatttgt ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa
    15841 gaggagcaac ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta
    15901 attaattcga ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt
    15961 ggttattgtt atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga
    16021 aggaaagccc aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac
    16081 aatctctgcc ccagtacggt gggtacgaaa tggagtagat tctgtccata atcttttttc
    16141 aacttatgcc gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca
    16201 gcaaaacaag aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca
    16261 gacgctgact agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg
    16321 gcaggacatg ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt
    16381 catgtcgcaa aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat
    16441 cgaattactg tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc
    16501 taatggcgaa gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt
    16561 gacccaatta actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct
    16621 tggagggaat tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag
    16681 ttatgggctg gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt
    16741 tgtgacgatt gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa
    16801 gtactatctg ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat
    16861 gctaggggag tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt
    16921 attatgttgc agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg
    16981 ggctatctat gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt
    17041 aattgtatgg ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt
    17101 tttcagtatg atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat
    17161 ttttaaatta gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact
    17221 gctgttgaac atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga
    17281 tgaataattt ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag
    17341 aaatgctaga cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat
    17401 ttttaaaagt tgttttgaaa catttttgta acataactat tatatcgttg acacactgct
    17461 atggtacaat ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta
    17521 agccagtcgg aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca
    17581 atgaccttga ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag
    17641 atacaacagc aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca
    17701 caaatcgatg ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta
    17761 gcaaaacaag atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta
    17821 caagaacgta ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta
    17881 agcaacacaa gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct
    17941 attggacgcg ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa
    18001 caaaaacaag ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa
    18061 gaattagctg aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa
    18121 gctgatttga acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag
    18181 aaagctgact tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa
    18241 caacaacgtc tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa
    18301 gcacgcgaac aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc
    18361 tcagcaacag aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct
    18421 acacaatctt cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca
    18481 gcgcctgaat cttcagcaac agaagaatca acaacag
//
LOCUS       DAZKCE010000056        17748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000056 DAZKCE010000000
VERSION     DAZKCE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.056"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..786)
                     /locus_tag="ACG8KQ_001708"
     CDS             complement(79..786)
                     /locus_tag="ACG8KQ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9104641.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="ACG8KQ_001709"
     CDS             complement(865..1203)
                     /locus_tag="ACG8KQ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9104642.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="ACG8KQ_001710"
     CDS             complement(1314..2276)
                     /locus_tag="ACG8KQ_001710"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9104643.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="ACG8KQ_001711"
     CDS             complement(2291..3622)
                     /locus_tag="ACG8KQ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9104644.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="ACG8KQ_001712"
     CDS             complement(3630..4310)
                     /locus_tag="ACG8KQ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9104645.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="ACG8KQ_001713"
     CDS             complement(4529..5008)
                     /locus_tag="ACG8KQ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9104646.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="ACG8KQ_001714"
     CDS             complement(5135..6814)
                     /locus_tag="ACG8KQ_001714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9104647.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="ACG8KQ_001715"
     CDS             7362..8864
                     /locus_tag="ACG8KQ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104648.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="ACG8KQ_001716"
     CDS             8877..9539
                     /locus_tag="ACG8KQ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104649.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="ACG8KQ_001717"
     CDS             complement(9640..9843)
                     /locus_tag="ACG8KQ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9104650.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="ACG8KQ_001718"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="ACG8KQ_001718"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9104651.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="ACG8KQ_001719"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="ACG8KQ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9104652.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="ACG8KQ_001720"
     CDS             complement(13807..14421)
                     /locus_tag="ACG8KQ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9104653.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="ACG8KQ_001721"
     CDS             complement(15027..15404)
                     /locus_tag="ACG8KQ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104654.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="ACG8KQ_001722"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="ACG8KQ_001722"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9104655.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(16628..17647)
                     /locus_tag="ACG8KQ_001723"
     CDS             complement(16628..17647)
                     /locus_tag="ACG8KQ_001723"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9104656.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         5117 a         3836 c         3017 g         5778 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag agaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcacttgct gttcctccta aacgacgatt aactattgat
    16621 catctattta acgaagagaa aattcattct ccatttttcc gtcacgtaac atgatttctt
    16681 ttgctgcttt tttgatatct tctccattat acaaaacatt atagattgtt tgagtaattg
    16741 gcatctcaac acctagctgt tgagctagct ctactgctgc ttttgtcgtt gccacaccct
    16801 caacgaccat tcccatgttt tctagaactt cttccagctt atgtccctgt cctaacaagt
    16861 taccggcacg ccaattgcga gaatgaacac ttgtacaagt aacgatcaag tcccccacac
    16921 cactcaaacc gataaatgtc agaggattgg cacccatcgc gacaccaaga cggctgattt
    16981 ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc tccgaatcct aatccatgga
    17041 tagcgccggc accaattgca atgatatttt tcagtgctgc accagtttct acgccaatca
    17101 catcattatt tgtataaatc ctgaaataat cattcatgaa cagtttttgt acataggctg
    17161 ctgctttttg atcaatactt gcagctgtaa ttgttgtgat atcatgtact gccacttcct
    17221 cagcatggct aggaccagat aatacaacaa ttgcctgtct tttttcttct ggaatttctt
    17281 cagccaaaac ttctgagatt cgtttatgcg tatcttgttc tagtccttta cttgcatgta
    17341 taatgattgg cttagtattc attttaccaa taagttcctg tgcgactgaa cgaatcgctt
    17401 ttgttggaac aacaaataaa atagcatctg cgttatccac tgcttctgcc aattctttat
    17461 atccgataat cgattctggg atcgtcaaat caggtaaaaa atgctgattt gtatgatact
    17521 tattgatttc atcaatctgt tcaggtacat ttccccaaat acggacatca tgcccgtttt
    17581 ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg accaagaact gcgatttttt
    17641 gtttcatgga tattcctcca aataaataat tgataatttc cattatagta tacatttctt
    17701 aaagttggga ctaatttcca ttgtttttaa gtgtttgttt acttcttt
//
LOCUS       DAZKCE010000057        17192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000057 DAZKCE010000000
VERSION     DAZKCE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.057"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG8KQ_001724"
     CDS             complement(33..1076)
                     /locus_tag="ACG8KQ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9104657.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG8KQ_001725"
     CDS             complement(1237..1920)
                     /locus_tag="ACG8KQ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFP9104658.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG8KQ_001726"
     CDS             2115..3044
                     /locus_tag="ACG8KQ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9104659.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8KQ_001727"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8KQ_001727"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9104660.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8KQ_001728"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8KQ_001728"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9104661.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8KQ_001729"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8KQ_001729"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9104662.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG8KQ_001730"
     CDS             5429..6232
                     /locus_tag="ACG8KQ_001730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9104663.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG8KQ_001731"
     CDS             6316..7863
                     /locus_tag="ACG8KQ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9104664.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG8KQ_001732"
     CDS             complement(8160..8357)
                     /locus_tag="ACG8KQ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104665.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG8KQ_001733"
     CDS             complement(8433..8633)
                     /locus_tag="ACG8KQ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9104666.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG8KQ_001734"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG8KQ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG8KQ_001735"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG8KQ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9104667.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ACG8KQ_001736"
     CDS             complement(11515..12297)
                     /locus_tag="ACG8KQ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104668.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ACG8KQ_001737"
     CDS             complement(12362..13198)
                     /locus_tag="ACG8KQ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9104669.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ACG8KQ_001738"
     CDS             complement(13216..14625)
                     /locus_tag="ACG8KQ_001738"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9104670.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="ACG8KQ_001739"
     CDS             complement(14639..16462)
                     /locus_tag="ACG8KQ_001739"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9104671.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         5108 a         3484 c         3199 g         5401 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa
    17101 taccaaacat atccataata attttttctt tttcatttct tttatattgt tataacagtg
    17161 aataacataa aaattcccga caatcattgt aa
//
LOCUS       DAZKCE010000058        16794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000058 DAZKCE010000000
VERSION     DAZKCE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.058"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG8KQ_001740"
     CDS             complement(<1..384)
                     /locus_tag="ACG8KQ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104672.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG8KQ_001741"
     CDS             complement(569..967)
                     /locus_tag="ACG8KQ_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9104673.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG8KQ_001742"
     CDS             complement(964..1737)
                     /locus_tag="ACG8KQ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9104674.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG8KQ_001743"
     CDS             complement(1734..2417)
                     /locus_tag="ACG8KQ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9104675.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG8KQ_001744"
     CDS             complement(2584..3471)
                     /locus_tag="ACG8KQ_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104676.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG8KQ_001745"
     CDS             complement(3468..4337)
                     /locus_tag="ACG8KQ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9104677.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG8KQ_001746"
     CDS             complement(4474..5760)
                     /locus_tag="ACG8KQ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9104678.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG8KQ_001747"
     CDS             6033..6611
                     /locus_tag="ACG8KQ_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9104679.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG8KQ_001748"
     CDS             6611..7540
                     /locus_tag="ACG8KQ_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104680.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG8KQ_001749"
     CDS             7537..9273
                     /locus_tag="ACG8KQ_001749"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9104681.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG8KQ_001750"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG8KQ_001750"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9104682.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG8KQ_001751"
     CDS             complement(10169..10678)
                     /locus_tag="ACG8KQ_001751"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9104683.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG8KQ_001752"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG8KQ_001752"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9104684.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG8KQ_001753"
     CDS             complement(11979..13070)
                     /locus_tag="ACG8KQ_001753"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9104685.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG8KQ_001754"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG8KQ_001754"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9104686.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG8KQ_001755"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG8KQ_001755"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9104687.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG8KQ_001756"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG8KQ_001756"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9104688.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4856 a         3708 c         2969 g         5261 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctga
//
LOCUS       DAZKCE010000059        16492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000059 DAZKCE010000000
VERSION     DAZKCE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.059"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8KQ_001757"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8KQ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9104689.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8KQ_001758"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8KQ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9104690.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8KQ_001759"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8KQ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9104691.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8KQ_001760"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8KQ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104692.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8KQ_001761"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8KQ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9104693.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8KQ_001762"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8KQ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9104694.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8KQ_001763"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8KQ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104695.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8KQ_001764"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8KQ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9104696.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8KQ_001765"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8KQ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9104697.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8KQ_001766"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8KQ_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9104698.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8KQ_001767"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8KQ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP9104699.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8KQ_001768"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8KQ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9104700.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8KQ_001769"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8KQ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9104701.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8KQ_001770"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8KQ_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9104702.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8KQ_001771"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8KQ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9104703.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8KQ_001772"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8KQ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFP9104704.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8KQ_001773"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8KQ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104705.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8KQ_001774"
     CDS             13967..14695
                     /locus_tag="ACG8KQ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFP9104706.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8KQ_001775"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8KQ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP9104707.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8KQ_001776"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8KQ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104708.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8KQ_001777"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8KQ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104709.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8KQ_001778"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8KQ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFP9104710.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8KQ_001779"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8KQ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104711.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4642 a         3473 c         2557 g         5820 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta at
//
LOCUS       DAZKCE010000060        16303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000060 DAZKCE010000000
VERSION     DAZKCE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.060"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8KQ_001780"
     CDS             14..1141
                     /locus_tag="ACG8KQ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9104712.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8KQ_001781"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8KQ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9104713.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG8KQ_001782"
     CDS             complement(2506..2691)
                     /locus_tag="ACG8KQ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9104714.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG8KQ_001783"
     CDS             complement(3045..3290)
                     /locus_tag="ACG8KQ_001783"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104715.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACG8KQ_001784"
     CDS             complement(3284..3685)
                     /locus_tag="ACG8KQ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9104716.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG8KQ_001785"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG8KQ_001785"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9104717.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="ACG8KQ_001786"
     CDS             complement(5703..7469)
                     /locus_tag="ACG8KQ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP9104718.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="ACG8KQ_001787"
     CDS             complement(7482..8276)
                     /locus_tag="ACG8KQ_001787"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9104719.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..9662)
                     /locus_tag="ACG8KQ_001788"
     CDS             complement(8286..9662)
                     /locus_tag="ACG8KQ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9104720.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9782..10429)
                     /locus_tag="ACG8KQ_001789"
     CDS             complement(9782..10429)
                     /locus_tag="ACG8KQ_001789"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFP9104721.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACG8KQ_001790"
     CDS             complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACG8KQ_001790"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9104722.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACG8KQ_001791"
     CDS             complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACG8KQ_001791"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9104723.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13096..14727)
                     /locus_tag="ACG8KQ_001792"
     CDS             complement(13096..14727)
                     /locus_tag="ACG8KQ_001792"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9104724.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15031..15702
                     /locus_tag="ACG8KQ_001793"
     CDS             15031..15702
                     /locus_tag="ACG8KQ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9104725.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15862..16053)
                     /locus_tag="ACG8KQ_001794"
                     /pseudo
     CDS             complement(15862..16053)
                     /locus_tag="ACG8KQ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5284 a         3155 c         2190 g         5674 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta
     8881 ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa
     8941 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
     9001 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
     9061 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
     9121 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
     9181 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
     9241 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
     9301 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
     9361 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
     9421 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
     9481 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
     9541 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
     9601 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
     9661 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
     9721 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
     9781 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
     9841 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
     9901 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     9961 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
    10021 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
    10081 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
    10141 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
    10201 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
    10261 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
    10321 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
    10381 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    10441 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
    10501 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
    10561 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
    10621 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
    10681 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    10741 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    10801 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    10861 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    10921 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    10981 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    11041 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    11101 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    11161 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    11221 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
    11281 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
    11341 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    11401 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
    11461 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
    11521 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
    11581 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    11641 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
    11701 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
    11761 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    11821 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
    11881 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    11941 atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa
    12001 ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    12061 aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa
    12121 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    12181 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga
    12241 gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    12301 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    12361 tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt
    12421 cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa
    12481 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    12541 agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt
    12601 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    12661 ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag
    12721 acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat
    12781 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    12841 caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct
    12901 caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt
    12961 ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    13021 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    13081 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    13141 aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc
    13201 aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca
    13261 atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc
    13321 taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa
    13381 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    13441 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    13501 tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac
    13561 ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc
    13621 taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat
    13681 tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct
    13741 tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc
    13801 agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac
    13861 tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa
    13921 atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat
    13981 tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    14041 tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg
    14101 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc
    14161 agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct
    14221 aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa
    14281 actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata
    14341 tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt
    14401 tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc
    14461 attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
    14521 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    14581 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
    14641 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
    14701 attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg
    14761 atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt
    14821 gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt
    14881 ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt
    14941 tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc
    15001 agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa
    15061 gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta
    15121 tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg
    15181 attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca
    15241 ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa
    15301 attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata
    15361 atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat
    15421 aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta
    15481 gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta
    15541 aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac aaaacaagat
    15601 ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa tgagcaaaat
    15661 attgttttag ctactcatcc aaaatatttc aaaaatatat aacttttaac tctttttaaa
    15721 ttaaatggtt gttctgatca gaaaaatatc gcttgttttc ttattatgta atatatttca
    15781 taaactattc tgcataacaa ctattgtttg tactttttac attggataac taattatgac
    15841 tgctgataac ttttgatatt aaactagtat ctccaataca aataaaaaat agatatcttg
    15901 ttcatcaaaa ttagttcgtg gatacttacc ttcatttaca tcattcagcg gtttcccatt
    15961 ttcttttatt ctattatttc attttctaat agacttggct tcatttcgca atactttttt
    16021 tcaataggca tcaaattggt gttgtttaga tatttagttg tagttttgtg acatgataag
    16081 tatgctcctt tttctctttt ccactaatac aacgatcaaa gggtgctttt ttcaaaaaac
    16141 tagaaaaatc aaaataagat tcgtattttg attctatata tcctgtttca actacatctt
    16201 atatttcaca atagaataaa tagccttttg catagctgct taacttataa gaaattgcaa
    16261 tttacttctt caaagtgaaa acgaatatga gtaaaataaa att
//
LOCUS       DAZKCE010000061        16012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000061 DAZKCE010000000
VERSION     DAZKCE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.061"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8KQ_001795"
     CDS             226..894
                     /locus_tag="ACG8KQ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9104726.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8KQ_001796"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8KQ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104727.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8KQ_001797"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8KQ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9104728.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8KQ_001798"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8KQ_001798"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9104729.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8KQ_001799"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8KQ_001799"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9104730.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8KQ_001800"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8KQ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9104731.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8KQ_001801"
     CDS             6859..7764
                     /locus_tag="ACG8KQ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9104732.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8KQ_001802"
     CDS             7855..8532
                     /locus_tag="ACG8KQ_001802"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9104733.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8KQ_001803"
     CDS             8529..8699
                     /locus_tag="ACG8KQ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104734.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8KQ_001804"
     CDS             8813..9709
                     /locus_tag="ACG8KQ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9104735.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8KQ_001805"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8KQ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9104736.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8KQ_001806"
     CDS             11372..12118
                     /locus_tag="ACG8KQ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9104737.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8KQ_001807"
     CDS             12115..12954
                     /locus_tag="ACG8KQ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9104738.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8KQ_001808"
     CDS             12955..13905
                     /locus_tag="ACG8KQ_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9104739.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8KQ_001809"
     CDS             14046..14798
                     /locus_tag="ACG8KQ_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104740.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8KQ_001810"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8KQ_001810"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9104741.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5193 a         2999 c         3226 g         4594 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tg
//
LOCUS       DAZKCE010000062        15743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000062 DAZKCE010000000
VERSION     DAZKCE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.062"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8KQ_001811"
     CDS             complement(24..722)
                     /locus_tag="ACG8KQ_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9104742.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8KQ_001812"
     CDS             complement(715..2067)
                     /locus_tag="ACG8KQ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9104743.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8KQ_001813"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8KQ_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9104744.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8KQ_001814"
     CDS             3851..4588
                     /locus_tag="ACG8KQ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9104745.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8KQ_001815"
     CDS             4713..5192
                     /locus_tag="ACG8KQ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9104746.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8KQ_001816"
     CDS             5189..5956
                     /locus_tag="ACG8KQ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9104747.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8KQ_001817"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8KQ_001817"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9104748.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8KQ_001818"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8KQ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9104749.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8KQ_001819"
     CDS             7567..7797
                     /locus_tag="ACG8KQ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104750.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8KQ_001820"
     CDS             7788..8036
                     /locus_tag="ACG8KQ_001820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104751.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8KQ_001821"
     CDS             8142..8726
                     /locus_tag="ACG8KQ_001821"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9104752.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8KQ_001822"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8KQ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9104753.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8KQ_001823"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8KQ_001823"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9104754.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8KQ_001824"
     CDS             10627..10806
                     /locus_tag="ACG8KQ_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104755.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8KQ_001825"
     CDS             10828..11853
                     /locus_tag="ACG8KQ_001825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9104756.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8KQ_001826"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8KQ_001826"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9104757.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8KQ_001827"
     CDS             13425..14372
                     /locus_tag="ACG8KQ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9104758.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8KQ_001828"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8KQ_001828"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9104759.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8KQ_001829"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8KQ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104760.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8KQ_001830"
     tRNA            15637..15709
                     /locus_tag="ACG8KQ_001830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DAZKCE010000063        15720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000063 DAZKCE010000000
VERSION     DAZKCE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.063"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1079)
                     /locus_tag="ACG8KQ_001831"
     CDS             complement(<1..1079)
                     /locus_tag="ACG8KQ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP9104761.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFF"
     gene            complement(1066..2754)
                     /locus_tag="ACG8KQ_001832"
     CDS             complement(1066..2754)
                     /locus_tag="ACG8KQ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104762.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2900..4537
                     /locus_tag="ACG8KQ_001833"
     CDS             2900..4537
                     /locus_tag="ACG8KQ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104763.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4682..5422)
                     /gene="truA"
                     /locus_tag="ACG8KQ_001834"
     CDS             complement(4682..5422)
                     /gene="truA"
                     /locus_tag="ACG8KQ_001834"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9104764.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5613..6248)
                     /locus_tag="ACG8KQ_001835"
     CDS             complement(5613..6248)
                     /locus_tag="ACG8KQ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9104765.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6395..7615)
                     /gene="sstT"
                     /locus_tag="ACG8KQ_001836"
     CDS             complement(6395..7615)
                     /gene="sstT"
                     /locus_tag="ACG8KQ_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9104766.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            7834..8832
                     /locus_tag="ACG8KQ_001837"
     CDS             7834..8832
                     /locus_tag="ACG8KQ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104767.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9028..10863
                     /locus_tag="ACG8KQ_001838"
     CDS             9028..10863
                     /locus_tag="ACG8KQ_001838"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9104768.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(10923..11639)
                     /locus_tag="ACG8KQ_001839"
     CDS             complement(10923..11639)
                     /locus_tag="ACG8KQ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9104769.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(11866..12408)
                     /locus_tag="ACG8KQ_001840"
     CDS             complement(11866..12408)
                     /locus_tag="ACG8KQ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9104770.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12413..12640)
                     /locus_tag="ACG8KQ_001841"
     CDS             complement(12413..12640)
                     /locus_tag="ACG8KQ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9104771.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(12719..14599)
                     /locus_tag="ACG8KQ_001842"
     CDS             complement(12719..14599)
                     /locus_tag="ACG8KQ_001842"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9104772.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(14788..15402)
                     /locus_tag="ACG8KQ_001843"
     CDS             complement(14788..15402)
                     /locus_tag="ACG8KQ_001843"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104773.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            15544..>15720
                     /locus_tag="ACG8KQ_001844"
     CDS             15544..>15720
                     /locus_tag="ACG8KQ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9104774.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         5016 a         2979 c         2462 g         5263 t
ORIGIN      
        1 ttgaaaaata tcatcgttaa tatcagagaa gagaatatta tttgcaagct taagatccgc
       61 caaggaaatt gtaacaattt ggctggcgta ttttgcagaa gataccatgg caccaaacta
      121 gttgtagcta gccaagtcaa acacgtacca taataaatct ttttttctat gtttgttaat
      181 tttttaaata aaatcagggg aatacataaa gataacagac caactagtcc cattgagtaa
      241 gtagttaagt cgttgttcat agcttcagtc agactatcga acacattaga tgcatgagca
      301 tataaatttg tcacgctagg tttgttgatt ttttggtaga gcatttgttc aaacatcgga
      361 taccaaaaag cactcgtcag aaccaatgtc attcctgccg cactaaataa ttttttaaat
      421 atagcaataa aatctgcatt cttgaaacct ttagaaacca aacgaataaa aataaaaatg
      481 ataattaatg ctgtactcat taatgttgat agtacatggc tgtacactag caaactcata
      541 ctcaatgcta gctttacaga cggatacttt tcttcttttc cagagatgat ttgataagca
      601 taaaataata cagggataac aagtgttatt gcgatagctt cggcaattgc ggaacgatag
      661 tagatgtcta ctgtccgata agcagagaaa atatagagac aactaaataa aaatgccgct
      721 tttttttgtt ttaaaaaccg ctcgccataa tgataagcta cgagtgttgt acacaagttc
      781 aataaccaaa cgtaaataat atagcttaca atcaaatttt ttgtcatcca atataacatt
      841 acagctggaa aaaaagtcag aaagggataa aaataattta caccatatcc agtataattg
      901 aatgtctcat aattaatcgg actggtaaaa atagtcgata accccttgat tcgagataag
      961 tgaaaagaaa gatctccagt ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa
     1021 tatatactac taactataat taatccactc aatattacta cttttttatt tattttcatt
     1081 cttcttgttt atctcctttt tagtcacata tcgagaatct ttcaacttat taggtgcaaa
     1141 tcgtttaaaa cttgaataca ccactaaaca ataagtcaga atactgatcc ataaagccat
     1201 gagagcagtt ggagtatatt ttgttgtaat ttctatctta ttttctccag catttacatc
     1261 cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt ttacttttac
     1321 ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt tgcttgccac
     1381 tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg attctcctac
     1441 ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg gggcgtaatc
     1501 gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat tgataacaat
     1561 atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta tagacgtgac
     1621 gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat tcatagtctt
     1681 tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac ctaagatacg
     1741 ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt
     1801 agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt ttttctccat
     1861 ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta
     1921 tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa gtgcttctag
     1981 ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct gcaacatagg
     2041 ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta aattcacgac
     2101 cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta taatcataca
     2161 agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa gtagacttat
     2221 actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat aagcataata
     2281 taaaacagct gggataatcg taagagcgat tgcttctgca attgcggatc gataatagat
     2341 atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag cagcatctat
     2401 ctttttaaaa aacttttctc cataataaaa actaatcaga atcgtacaaa tatttaataa
     2461 ccaaatatat agaacataac ttaatattaa gttatttgaa attccataaa aaacgactgc
     2521 aggaattatt gtaagaaaag gataaaaata gttcagtcca tctccataat tgttaaatgt
     2581 tgtatagttg attggccctt caaaaataga agataaccct ttgattcgtg aaagatgaaa
     2641 agataaatca cctgttgtat aaatttcttg agcatttctc ataaaattta ctgaatataa
     2701 agtactaagt aagataagaa ctactaaaag aaatgctttt ttacttattt tcatagattt
     2761 gttgttctcc ttttttaatt ctattttcag tatactagaa ttgtcttttt ttaataagtt
     2821 ttgcgttaaa tttttcatag cactagtatg atatactaga aaagaatcaa tttttattac
     2881 ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata tcatttttat
     2941 gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa tctatcatag
     3001 tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata atgtatggag
     3061 ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata ttttttatcc
     3121 atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc ttgttttaag
     3181 ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt atttttctat
     3241 gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt atacattttc
     3301 cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg tcgctttaac
     3361 atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt accaaaaatg
     3421 gtactggttg tctattggca tgacgctagt agtgtatact catttacttt ctgtcgctat
     3481 ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg accaaaaaat
     3541 cgctagactc ttaagtcttt taaaagctac cgtcctcacc ttttttttaa gtgcgggatt
     3601 cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac tgggaaaaga
     3661 attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata actataacaa
     3721 ctatactatt ggattattct tatttctcgg attgattggt gcttttattt ttataaaaaa
     3781 attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt tttgttcaac
     3841 caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac aatttgtctg
     3901 gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag tgatctatta
     3961 tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc ttgcactaat
     4021 attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat
     4081 agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa
     4141 agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga
     4201 aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa
     4261 taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat
     4321 taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc
     4381 tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa ttgcttcaag
     4441 atatttatct atcgttactt tgattttatt tttactttat cgatttactt tttctaaaca
     4501 aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag aaagactttt
     4561 tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat ttgcttcata
     4621 cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta tcaaagtatt
     4681 tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt tctcctgcat
     4741 gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg gcacctgtgc
     4801 cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat ccgtctccga
     4861 caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca atcgtacgca
     4921 ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta
     4981 atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa taaaagctgt
     5041 ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg tattttttgc
     5101 ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt ttgatgacaa
     5161 tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca atttctgtgt
     5221 ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc
     5281 caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt tttccttgaa
     5341 tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg tcatattcga
     5401 ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt ggtcttctct
     5461 ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga cagaagctat
     5521 acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc ggatgaagta
     5581 tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa gatactcaac
     5641 caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc
     5701 agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc
     5761 aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc attttttcga
     5821 tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa acctgatccg
     5881 atattatgga cccatgtcga gacattccac gaagattcat gggtattgat actgaacaaa
     5941 cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac tagggtagct
     6001 aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag gatgaaaaag
     6061 atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga aacctctcca
     6121 aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc taatacgtac
     6181 ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa
     6241 aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat tataaacgac
     6301 tttatcttga tttaacagaa acctctattt acacgaacat ctatataact cattaaaaac
     6361 tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat ccatctaaac
     6421 aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct cctattgcag
     6481 taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg ataacaccga
     6541 taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca aacaaactac
     6601 atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca caagcagcta
     6661 aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc cctaaagaat
     6721 gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata ttgatcgtag
     6781 cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt tcagccaaca
     6841 tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt
     6901 ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa aacgtgagga
     6961 atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt aaaagctgca
     7021 gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc aatcctaaaa
     7081 tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct atagatatct
     7141 gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc aacaatacag
     7201 cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga ttttgtgtga
     7261 cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt tcagatactg
     7321 tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct acaagtgcag
     7381 aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc ttattcccaa
     7441 ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt gcgattgcct
     7501 tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga
     7561 taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac gacatatcca
     7621 aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt atcattttat
     7681 gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa ccatttcttg
     7741 ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag ttaggatgtt
     7801 attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg
     7861 tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat ctttgtcgct
     7921 tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc tacatatgca
     7981 acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt tattggtgca
     8041 aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta
     8101 cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg caatatcttt
     8161 ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc
     8221 agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat
     8281 acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact attattttta
     8341 acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat
     8401 ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg
     8461 aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta
     8521 ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca
     8581 gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt
     8641 ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct
     8701 gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct
     8761 acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag
     8821 ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa
     8881 aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta
     8941 actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt
     9001 gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc aaaagaaggt
     9061 tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc
     9121 ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt
     9181 tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc
     9241 aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct gaccggattg
     9301 tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc
     9361 ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag
     9421 attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc
     9481 tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac
     9541 aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta
     9601 aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat
     9661 ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag aagtgactgg
     9721 ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc
     9781 cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga
     9841 acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa
     9901 ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag
     9961 ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc
    10021 atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt
    10081 gacaggttct attatttaga cagtccggat aaattaactt atacggataa aatagaacaa
    10141 agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat
    10201 acggaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat
    10261 tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa
    10321 gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc
    10381 caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc
    10441 gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat
    10501 ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt
    10561 gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac
    10621 tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa
    10681 agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc
    10741 caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa
    10801 tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aataaaagac
    10861 taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc ttgtttatct
    10921 cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc attattcgct
    10981 gtctcgttga agtaaagata attatcggga ttcccctgat tttgttgtgt caccaatact
    11041 tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata tggttctgtc
    11101 tctgcatcgg aatagacggc tgtgatttga tagtctgctt gacctgtccc actttctgac
    11161 cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt caaaatagct
    11221 tgagggactt taagaccata taagtccaca ctcatttgat tcgtataact tttgtattgt
    11281 tgtcccattg tttggcccca tttggtcata aatgcctcta attgtgcgga tttgtctgca
    11341 ttccacagtg tttgttcaac ttgttcgctg ctttctgaag aggaagatga agcctctaca
    11401 gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc tgacgaagta
    11461 gaggtatctg ctgtttctgt acttgctgca gttgacttgc tctctgtgct agttgttgta
    11521 gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt cttttttcga ttcattcccg
    11581 ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct ttttttcaat
    11641 taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca atgtgatttt
    11701 attgattttt cgtattttct catcaatctt tcaaatcttc taaaaactga gaagcacccg
    11761 ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc gtgattttcc
    11821 aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt catcatcgtc
    11881 ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt ggtagatgat
    11941 ttctttattg aaacttaaaa gttcaacaag caattgttgc aagccatatt tttcttcttt
    12001 ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa
    12061 gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt aaatttttcc
    12121 ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca ctaactcatg
    12181 agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa
    12241 ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg atcaatttca tttcttctaa
    12301 tttaaaattt gctaatacat gccccaccat ggcgccagca gttggtttat gatggtcgat
    12361 atctgcttgt tttgtttctt gaacaaattt ttcttccact atattcattg atttacgcct
    12421 cttttacttt tgctgattgg acttcataac ctaatttttc cacaactgca gctaattgtt
    12481 cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta aacaaaactt
    12541 tcgtattgct taccccaggc tgttgagaaa ccgcatgttc gattttttgc atacagcttg
    12601 gacaagctag tgtatctaat tgcataataa tttttttcat cgtagttctt ccttcctttt
    12661 tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt atcaagtttt
    12721 acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa
    12781 cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata aacccggcaa
    12841 ataagccgtt gagtaaaaat aagacgacac caatggcaat cactacattt tcacgggtat
    12901 ttaaaacagt tttcttagcc aagcggtagg catgaaccaa cgattcaaaa ttagattgca
    12961 tcaagacaac gtcactcgtt tcgatagcaa catccgttcc tgatcccata gcaatcccga
    13021 tatcagccgc agccaaagag ggagaatcat tgattccatc tccaataaaa gcaacgcgca
    13081 atccttgttc ttggtatttt ttcacaaatt gaaccttttc gtctggtaat aattcagcat
    13141 ggacttcatc aatgcctaac atatcagcta cataatcagc agtcatctga ttatcacctg
    13201 tcagcatcac taactgtttt gctcctgctt gtttcaactt agctaattgt tcagcgactt
    13261 ctggtcgaat cacatctgac acacctatga tttgagtgat ttttttgtcg attgctacaa
    13321 taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga
    13381 gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta tgaccatcaa
    13441 tctcagctgt gattccttgt cccttgattg tctgattatc tttgactgct aattgttgaa
    13501 aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga tgatcggata
    13561 acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta tagttcttga
    13621 tagtggatac agcagtagtt cctttggtca atgtaccagt tttatcaaag acaaaagtat
    13681 cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc cgtttcgctc
    13741 cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac
    13801 caagaaccaa aacagtgatt gctaaacgaa aatcacgact aatcagacca acaaccaacg
    13861 caataagtaa aacggttgga gtataatact gagaaaatcg atcaatgaat ttttccgcat
    13921 gggatttggt atcttgtgct tcttctacta attcgataat tttggcaaag gtcgtatcat
    13981 ctccgacctt agttgcttca atcgtcacgt agccatctgc taaaatcgtt ccagagaaga
    14041 cttcatctcc actgattttg acgacttgtc gtgattcacc agtcactgcg gcttcatccg
    14101 catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca gtttttacta
    14161 agacgatatc gccttcttcc acgtcatcga tttccacttc ttcagtcgtt ccatcttctt
    14221 ggacaacact agcagtagat ggggccatct cggtcaaagc cttgattgac tcccgcgttt
    14281 tggtcaatgt tttttgttcc agatagctac caaacaaaaa caagaatgta acgatagctg
    14341 actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg agtaaatcaa
    14401 ttgagataac tttgactttc actgcctgat acgcatggat cataataggt atgaccgcaa
    14461 taatagaagc tatggctaga gcaatgttat agaacaagac aaaaccagta aattttccga
    14521 taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga tgctcttgga
    14581 tccacttttg taatttcatc tttctccctc ttttcctttc attcttacga gactaagtat
    14641 agatacttat ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa ggcggttttc
    14701 ttttggtaga atacactaca aaaaatagtt agcaagaaga ctcactaaaa gtgatcttgc
    14761 caactattgt cgtttctatc gaaattttca ttgaatgatt ttcctgtaca gcatacatcg
    14821 gagatctatt tctttatcta agatttttat cttgctaatt ttttccttta gtctttcaaa
    14881 tccacgtcgt tcatagaatt gatacgtaca gccactatct gtaaaaagga atatttcttt
    14941 tcctttctct cttttttcaa attctgttag caacttggta ccgatgcctt ttgttttcaa
    15001 atcaggattt gcagctaaaa aaatgatctc tccatctggc gaatgcttct ttaaatatgc
    15061 tgcaagcatt tgttcatttg ctgtctcgta agctccttta ctttctttag caaatatatt
    15121 ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca aaagaacgat gtttcttttt
    15181 ttctcctttt atctccgcca gcaaaacacc tgccaactcg tcctctgcat acaaagcaag
    15241 aatttgagtt gctcgattca actccatata caaaaaatat cttccgtaaa gattcagcag
    15301 aagttcattt tccatatatc tctcaaagtt cattccttca acagcaaaat agatagcttt
    15361 tttgtaatct ttcttttcta agggtttaaa agttagtttc atcatttcgc ctcctcaact
    15421 tcaatgattg tgttatactt acattataat agacacagtg tctactatct ataatcaatc
    15481 atctataagt ttgtcgatta agcgacagct ttgctttgat atgtcggata aggagatagg
    15541 taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa aagctttggc tgaaacacta
    15601 tccacattat tagtaaataa aaagattcaa gcaatcacta tacgcgaact aacagaaacg
    15661 gcagatgtac atcgttcgac tttttataca cattataagg atatctatga tctatatgat
//
LOCUS       DAZKCE010000064        15710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000064 DAZKCE010000000
VERSION     DAZKCE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.064"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1462
                     /locus_tag="ACG8KQ_001845"
     CDS             35..1462
                     /locus_tag="ACG8KQ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104775.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1554..1919)
                     /locus_tag="ACG8KQ_001846"
     CDS             complement(1554..1919)
                     /locus_tag="ACG8KQ_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9104776.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1916..2281)
                     /locus_tag="ACG8KQ_001847"
     CDS             complement(1916..2281)
                     /locus_tag="ACG8KQ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9104777.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2473..2721)
                     /locus_tag="ACG8KQ_001848"
     CDS             complement(2473..2721)
                     /locus_tag="ACG8KQ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104778.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2720..2848
                     /locus_tag="ACG8KQ_001849"
     CDS             2720..2848
                     /locus_tag="ACG8KQ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9104779.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2905..4599
                     /locus_tag="ACG8KQ_001850"
     CDS             2905..4599
                     /locus_tag="ACG8KQ_001850"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9104780.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4699..4872
                     /locus_tag="ACG8KQ_001851"
     CDS             4699..4872
                     /locus_tag="ACG8KQ_001851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104781.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5250..6383
                     /locus_tag="ACG8KQ_001852"
     CDS             5250..6383
                     /locus_tag="ACG8KQ_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9104782.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6414..6734
                     /locus_tag="ACG8KQ_001853"
     CDS             6414..6734
                     /locus_tag="ACG8KQ_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9104783.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6763..7227)
                     /locus_tag="ACG8KQ_001854"
     CDS             complement(6763..7227)
                     /locus_tag="ACG8KQ_001854"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104784.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7359..8612)
                     /locus_tag="ACG8KQ_001855"
     CDS             complement(7359..8612)
                     /locus_tag="ACG8KQ_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9104785.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8641..8958)
                     /locus_tag="ACG8KQ_001856"
     CDS             complement(8641..8958)
                     /locus_tag="ACG8KQ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9104786.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8984..9304)
                     /locus_tag="ACG8KQ_001857"
     CDS             complement(8984..9304)
                     /locus_tag="ACG8KQ_001857"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9104787.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9768..11183)
                     /locus_tag="ACG8KQ_001858"
     CDS             complement(9768..11183)
                     /locus_tag="ACG8KQ_001858"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9104788.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11200..12516)
                     /locus_tag="ACG8KQ_001859"
     CDS             complement(11200..12516)
                     /locus_tag="ACG8KQ_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9104789.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12666..13364)
                     /locus_tag="ACG8KQ_001860"
     CDS             complement(12666..13364)
                     /locus_tag="ACG8KQ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9104790.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13653..14642)
                     /locus_tag="ACG8KQ_001861"
     CDS             complement(13653..14642)
                     /locus_tag="ACG8KQ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9104791.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14722..15555)
                     /gene="msrA"
                     /locus_tag="ACG8KQ_001862"
     CDS             complement(14722..15555)
                     /gene="msrA"
                     /locus_tag="ACG8KQ_001862"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9104792.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4882 a         3186 c         2690 g         4952 t
ORIGIN      
        1 attgtgcaaa aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa
       61 tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga
      121 aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat
      181 aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt
      241 atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt
      301 gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac
      361 tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta
      421 ttttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag
      481 acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc
      541 tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag
      601 attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg
      661 ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc
      721 tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa
      781 agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt tccattctta
      841 tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga
      901 aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca
      961 accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt
     1021 catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca
     1081 gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg
     1141 catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac
     1201 tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc
     1261 tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa
     1321 gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga
     1381 tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat
     1441 ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg
     1501 ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta
     1561 ttttggacat ctacgggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata
     1621 actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg
     1681 atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta
     1741 atagcattgt tctcgctgac tcggtcgctt tgcacttttg tatggacaaa taaatatttc
     1801 aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca
     1861 agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc
     1921 ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa
     1981 tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga
     2041 taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg
     2101 ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat
     2161 gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag
     2221 aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca
     2281 tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc
     2341 ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca
     2401 ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag
     2461 ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt
     2521 tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac
     2581 cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc
     2641 ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga
     2701 aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt
     2761 gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca
     2821 tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac
     2881 ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt
     2941 attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag
     3001 atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta
     3061 gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga
     3121 ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata
     3181 ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa
     3241 caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga
     3301 aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat
     3361 tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac
     3421 ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt
     3481 tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa
     3541 catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg
     3601 atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg
     3661 gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat
     3721 acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact
     3781 aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat
     3841 atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg
     3901 ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg
     3961 acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga
     4021 aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc
     4081 aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca
     4141 gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat
     4201 attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat
     4261 cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat
     4321 atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga
     4381 ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag
     4441 ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc
     4501 cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt
     4561 cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct
     4621 gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg
     4681 ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4741 gctgccctgc atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact
     4801 tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa
     4861 aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg
     4921 cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat
     4981 gtctgtttct ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat
     5041 catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc
     5101 tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat
     5161 tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca
     5221 attttatgaa tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga
     5281 aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc
     5341 gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag
     5401 ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat
     5461 ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg
     5521 gtcattgtca tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc
     5581 agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag
     5641 cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc
     5701 ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg
     5761 aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc
     5821 tattcgctca aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag
     5881 tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag
     5941 acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta
     6001 tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa
     6061 gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg
     6121 gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat
     6181 tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa
     6241 aaatttacgc aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc
     6301 tgccactatc agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata
     6361 taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca
     6421 aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag
     6481 gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt
     6541 tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc
     6601 gatatggcac ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca
     6661 gaatcgtaag gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa
     6721 aaaaagaggc atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact
     6781 ttgatacaga cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc
     6841 gtatcgttca taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg
     6901 atgcatttct tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc
     6961 cggttcaaca aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt
     7021 ttcaatgacg cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg
     7081 aggaatactt tccttattag cgattccagt acgaatactt tcctcataca caacagcagg
     7141 aactccccat tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct
     7201 taatttgatc cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac
     7261 aaaaattata caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa
     7321 aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt
     7381 cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc
     7441 aaattgacag cggctagtac accacctgca atactttttg ttgccaatac accgccaata
     7501 attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc
     7561 gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc
     7621 gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc
     7681 gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata
     7741 gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa
     7801 ggtttgttca cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca
     7861 atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca
     7921 aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca
     7981 agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg
     8041 aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt
     8101 aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat
     8161 ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca
     8221 ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa
     8281 gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc
     8341 aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat
     8401 cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt
     8461 aagttattga tcattactgc taatgctcct aagatcatta acggcatact caccataaat
     8521 gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa
     8581 tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga
     8641 ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat
     8701 agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc
     8761 ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc
     8821 tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc
     8881 ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag
     8941 atttttttga tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt
     9001 tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta
     9061 catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta
     9121 cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg
     9181 aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt
     9241 ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt
     9301 ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac
     9361 tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac
     9421 acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt
     9481 taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt
     9541 attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt
     9601 tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac
     9661 ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta
     9721 tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt
     9781 tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt
     9841 ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta
     9901 aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt
     9961 gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg
    10021 taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata
    10081 ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata catggctata
    10141 tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt
    10201 aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa
    10261 cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga
    10321 tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac
    10381 aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta
    10441 taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag
    10501 accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc
    10561 tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg
    10621 ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt
    10681 tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca
    10741 tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga
    10801 aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa
    10861 gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca gttctcataa
    10921 tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc
    10981 atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac
    11041 ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct
    11101 tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa
    11161 ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac
    11221 acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag
    11281 ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg
    11341 cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata
    11401 accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag
    11461 catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga
    11521 taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa
    11581 accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat
    11641 aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa
    11701 aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa
    11761 gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa
    11821 tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat
    11881 gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa
    11941 actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt
    12001 cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc
    12061 tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt
    12121 cactaattca taaacagttg gattaacaga taaataagac atcactgcga tgataccagg
    12181 gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata
    12241 gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc
    12301 taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga
    12361 aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt
    12421 tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc
    12481 taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca
    12541 tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct
    12601 tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg
    12661 aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc
    12721 atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg
    12781 atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta
    12841 aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta
    12901 gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct
    12961 cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt
    13021 gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag
    13081 tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc
    13141 cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa
    13201 gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat
    13261 tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg
    13321 atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt
    13381 tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga tgattttttg
    13441 tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa
    13501 aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat
    13561 atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta
    13621 atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat
    13681 tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt
    13741 aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg
    13801 gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt
    13861 tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta
    13921 gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg
    13981 aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt
    14041 gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt
    14101 aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat
    14161 gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca
    14221 gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct ttggagactt
    14281 tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac
    14341 atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct
    14401 ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca
    14461 aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg
    14521 ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa
    14581 cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac
    14641 aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta
    14701 ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt
    14761 gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg
    14821 tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc
    14881 tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt
    14941 tgattgttcc gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg
    15001 cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat
    15061 tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac
    15121 atgacctgtg taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt
    15181 gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca
    15241 gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt
    15301 ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat
    15361 tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga
    15421 taactgttct ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt
    15481 tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat
    15541 ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg
    15601 taatttatca taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat
    15661 aaaaaagaga gaatccttat cgacagattc tcctttttaa tcaatttatt
//
LOCUS       DAZKCE010000065        15617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000065 DAZKCE010000000
VERSION     DAZKCE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.065"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..2477)
                     /locus_tag="ACG8KQ_001863"
     CDS             complement(132..2477)
                     /locus_tag="ACG8KQ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9104793.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="ACG8KQ_001864"
     CDS             2756..3658
                     /locus_tag="ACG8KQ_001864"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9104794.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="ACG8KQ_001865"
     CDS             3869..5644
                     /locus_tag="ACG8KQ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9104795.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="ACG8KQ_001866"
     CDS             complement(5710..7296)
                     /locus_tag="ACG8KQ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9104796.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="ACG8KQ_001867"
     CDS             7547..7771
                     /locus_tag="ACG8KQ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9104797.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="ACG8KQ_001868"
     CDS             7913..9664
                     /locus_tag="ACG8KQ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9104798.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="ACG8KQ_001869"
     CDS             9664..11448
                     /locus_tag="ACG8KQ_001869"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104799.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /gene="licT"
                     /locus_tag="ACG8KQ_001870"
                     /pseudo
     CDS             11783..12635
                     /gene="licT"
                     /locus_tag="ACG8KQ_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12732..14618
                     /locus_tag="ACG8KQ_001871"
     CDS             12732..14618
                     /locus_tag="ACG8KQ_001871"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9104800.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="ACG8KQ_001872"
     CDS             complement(14659..15561)
                     /locus_tag="ACG8KQ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9104801.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         4794 a         2858 c         3233 g         4732 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgattt
//
LOCUS       DAZKCE010000066        15505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000066 DAZKCE010000000
VERSION     DAZKCE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.066"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8KQ_001873"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8KQ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8KQ_001874"
     CDS             706..1734
                     /locus_tag="ACG8KQ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9104802.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8KQ_001875"
     CDS             1731..2489
                     /locus_tag="ACG8KQ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9104803.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8KQ_001876"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8KQ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9104804.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8KQ_001877"
     CDS             3376..4629
                     /locus_tag="ACG8KQ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9104805.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8KQ_001878"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8KQ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8KQ_001879"
     CDS             5300..5626
                     /locus_tag="ACG8KQ_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9104806.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8KQ_001880"
     CDS             5694..6407
                     /locus_tag="ACG8KQ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9104807.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8KQ_001881"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8KQ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8KQ_001882"
     CDS             7690..9555
                     /locus_tag="ACG8KQ_001882"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9104808.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8KQ_001883"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8KQ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8KQ_001884"
     CDS             11120..11821
                     /locus_tag="ACG8KQ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9104809.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8KQ_001885"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8KQ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG8KQ_001886"
     CDS             complement(12857..13066)
                     /locus_tag="ACG8KQ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP9104810.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG8KQ_001887"
     CDS             13307..13882
                     /locus_tag="ACG8KQ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9104811.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG8KQ_001888"
     CDS             13882..14322
                     /locus_tag="ACG8KQ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9104812.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG8KQ_001889"
     CDS             14338..15039
                     /locus_tag="ACG8KQ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9104813.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            15211..>15505
                     /locus_tag="ACG8KQ_001890"
     CDS             15211..>15505
                     /locus_tag="ACG8KQ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9104814.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT         5231 a         2574 c         3265 g         4435 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa ttacggctct ataatttatg ggactgatga atcagaattg attcttcagc
    15121 cccattttta ttttaggtat taaaaaacat atcgttctct agtatgatta aatcacgaca
    15181 aaataaacaa ctgagaggac gatatgctat atgaatgatt ctatcaaaaa aatgctgcga
    15241 ttaatagaga aagatttgat gattacagag gtctcttacg agacctttca gaagaaaaag
    15301 acgttgatcg tcgatgctgt tttctcacct gctccccata cctgtagaaa ttgtggttct
    15361 actgtggtag atggaaacgg gaaagtaatt gtagtgaaaa atgggaagaa ggaaacgatt
    15421 gtccgctttg aacaatacaa tcacatgcct ttggttatgc gcttaaaaaa gcagcgctat
    15481 acctgtaaaa actgtcgaac ccatt
//
LOCUS       DAZKCE010000067        15423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000067 DAZKCE010000000
VERSION     DAZKCE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.067"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8KQ_001891"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8KQ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9104815.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8KQ_001892"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8KQ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9104816.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG8KQ_001893"
     CDS             1874..2212
                     /locus_tag="ACG8KQ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104817.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG8KQ_001894"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG8KQ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG8KQ_001895"
     CDS             complement(3711..4292)
                     /locus_tag="ACG8KQ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9104818.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG8KQ_001896"
     CDS             complement(4475..5101)
                     /locus_tag="ACG8KQ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9104819.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG8KQ_001897"
     CDS             complement(5123..6454)
                     /locus_tag="ACG8KQ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9104820.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG8KQ_001898"
     CDS             complement(6609..7010)
                     /locus_tag="ACG8KQ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9104821.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG8KQ_001899"
     CDS             complement(7096..7302)
                     /locus_tag="ACG8KQ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104822.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG8KQ_001900"
     CDS             complement(7397..8899)
                     /locus_tag="ACG8KQ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9104823.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8KQ_001901"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8KQ_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9104824.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG8KQ_001902"
     CDS             complement(9470..10087)
                     /locus_tag="ACG8KQ_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9104825.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG8KQ_001903"
     CDS             10308..11681
                     /locus_tag="ACG8KQ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9104826.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG8KQ_001904"
     CDS             11756..12868
                     /locus_tag="ACG8KQ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9104827.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG8KQ_001905"
     CDS             complement(12940..14577)
                     /locus_tag="ACG8KQ_001905"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9104828.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG8KQ_001906"
     CDS             complement(14745..15353)
                     /locus_tag="ACG8KQ_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9104829.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4267 a         3166 c         2689 g         5301 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tca
//
LOCUS       DAZKCE010000068        15252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000068 DAZKCE010000000
VERSION     DAZKCE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.068"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1018
                     /locus_tag="ACG8KQ_001907"
     CDS             <1..1018
                     /locus_tag="ACG8KQ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9104830.1"
                     /translation="SIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLITG
                     KLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFFT
                     SIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMII
                     SILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVF
                     LSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILL
                     FVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVR
                     RLKR"
     gene            1015..1782
                     /locus_tag="ACG8KQ_001908"
     CDS             1015..1782
                     /locus_tag="ACG8KQ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9104831.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1882..3450
                     /gene="liaX"
                     /locus_tag="ACG8KQ_001909"
     CDS             1882..3450
                     /gene="liaX"
                     /locus_tag="ACG8KQ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9104832.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3747..4067
                     /locus_tag="ACG8KQ_001910"
     CDS             3747..4067
                     /locus_tag="ACG8KQ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9104833.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4067..4417
                     /locus_tag="ACG8KQ_001911"
     CDS             4067..4417
                     /locus_tag="ACG8KQ_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9104834.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4584..5522
                     /gene="hprK"
                     /locus_tag="ACG8KQ_001912"
     CDS             4584..5522
                     /gene="hprK"
                     /locus_tag="ACG8KQ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9104835.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5537..6367
                     /gene="lgt"
                     /locus_tag="ACG8KQ_001913"
     CDS             5537..6367
                     /gene="lgt"
                     /locus_tag="ACG8KQ_001913"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9104836.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6411..7436
                     /locus_tag="ACG8KQ_001914"
     CDS             6411..7436
                     /locus_tag="ACG8KQ_001914"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9104837.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7451..8389
                     /gene="galU"
                     /locus_tag="ACG8KQ_001915"
     CDS             7451..8389
                     /gene="galU"
                     /locus_tag="ACG8KQ_001915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9104838.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8514..8954
                     /locus_tag="ACG8KQ_001916"
     CDS             8514..8954
                     /locus_tag="ACG8KQ_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9104839.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8957..9559
                     /locus_tag="ACG8KQ_001917"
     CDS             8957..9559
                     /locus_tag="ACG8KQ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9104840.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9622..10725)
                     /locus_tag="ACG8KQ_001918"
     CDS             complement(9622..10725)
                     /locus_tag="ACG8KQ_001918"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9104841.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10935..11936
                     /gene="ccpA"
                     /locus_tag="ACG8KQ_001919"
     CDS             10935..11936
                     /gene="ccpA"
                     /locus_tag="ACG8KQ_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9104842.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12291..12686
                     /locus_tag="ACG8KQ_001920"
     CDS             12291..12686
                     /locus_tag="ACG8KQ_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104843.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12686..12937
                     /locus_tag="ACG8KQ_001921"
     CDS             12686..12937
                     /locus_tag="ACG8KQ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104844.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12980..13375
                     /locus_tag="ACG8KQ_001922"
     CDS             12980..13375
                     /locus_tag="ACG8KQ_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104845.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13372..14628
                     /locus_tag="ACG8KQ_001923"
     CDS             13372..14628
                     /locus_tag="ACG8KQ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFP9104846.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14640..14963
                     /locus_tag="ACG8KQ_001924"
     CDS             14640..14963
                     /locus_tag="ACG8KQ_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104847.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14956..15222
                     /locus_tag="ACG8KQ_001925"
     CDS             14956..15222
                     /locus_tag="ACG8KQ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104848.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5240 a         2580 c         3225 g         4207 t
ORIGIN      
        1 tagcatcatc actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc
       61 cgctttatat gaagtacctg tcatcgagat agaaaaagta cgaataaaag aggatcatca
      121 attgattaca ggaaaactga tgaactcgga tcaaaaagga gaatcgattt atttatcggt
      181 tccttatcaa aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt
      241 ccaaatgcat ggagatgaat ggcagctagt agcaaaaaaa aatgatggct ggcttttctt
      301 cttcacaagt atcttcattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc
      361 tttaggaagt ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa
      421 aacagaaggg atttctctat taaataaatc tatcctgttc gtcgttatag ggatgattat
      481 tagcatattg gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt
      541 tacgagtgtt tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca
      601 aggactgcgc tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc
      661 tagcgtactg attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac
      721 attagaagaa ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg
      781 aaggcaagtg ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat
      841 cagtgggtca gttccatcgc ttcttttata tttaggaaat caatggtctt tcaaagaaac
      901 gatagaacag catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt
      961 attatcgatc cccatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat
     1021 gtacttatta tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa
     1081 ggtatgtata ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc
     1141 ggttatcatc agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct
     1201 gtggttatct tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt
     1261 atactgatct ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc
     1321 caaggtttta catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt
     1381 gattttagat tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat
     1441 gcgagtgtag caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct
     1501 ttttaccaat tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta
     1561 atgacacttc tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat
     1621 ttagacattc cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg
     1681 gctattctta ccacattgtc cgcactaatt gttcttcctt taacctgcgt gactacagca
     1741 tatctcttta caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc
     1801 tatgacattt ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt
     1861 ttgcttaagg aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa
     1921 ggaatccttt cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac
     1981 gaaaagcaaa tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagataag
     2041 gcaagtcaat tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat
     2101 ccaaaggaaa gagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca
     2161 actgaagcga ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat
     2221 aaggcagcac gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta
     2281 ggcgagttga cagaagaaga attagcacag cgtcaggcgt tagaggcaga aatcaaagag
     2341 ttagaagcta gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa
     2401 gatatccgta agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg
     2461 ccagatgatt ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca
     2521 gaagctggat cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca
     2581 gtgaatgaca acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg
     2641 aaatttgagc atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca
     2701 gcaaacggaa acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag
     2761 atcaaacttt acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag
     2821 attgaggtta atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac
     2881 cttgttttct acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc
     2941 aacatcatga ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac
     3001 atcatcgtgg accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc
     3061 gatattcgaa acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca
     3121 tttggtgcga cagcagaaaa tctttctgtg tctttagtaa atggtgatgt acgtcttacg
     3181 attaaagaag ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc
     3241 gctttaccag atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaat
     3301 agccgtctgt ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg
     3361 caattccgtc gtgtttcaga tgatgagatc gcgcaagtgc aactttcaac gacaacagga
     3421 agtatctatt taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga
     3481 gcaataagaa gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc
     3541 cgatagtcta tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg
     3601 agtcacaagg aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga
     3661 ccgagaattt tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat
     3721 ttaaaatagt agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa
     3781 atgttgtttt gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg
     3841 ttatccgtgt gctttatgta ttcctgagct tagttgcttt tggaagtcca attattttat
     3901 acattttatt agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata
     3961 acaatgcata ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac
     4021 gcaaagaagc ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca
     4081 acgtatttta gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt
     4141 ccatgtgagc agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat
     4201 gtttgtcaaa ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt
     4261 gtttacgttt gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga
     4321 acattttagt ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt
     4381 caatgtaatt gtcagcgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg
     4441 gcgcagtttt tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa
     4501 aacgtttttt ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact
     4561 ctaatagaaa tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa
     4621 aattatcttt ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg
     4681 gggacatctc acgcccagga ctagagttga ctggttattt taattattat tcccataatc
     4741 gtttacagct atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc
     4801 gtttgatcat catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag
     4861 gattaccagc tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc
     4921 gttctcccat ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc
     4981 ttgcccctcg aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga
     5041 ttcaaggtga cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggcc
     5101 atcgattgat cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg
     5161 gagaaccgcc taaaatcctg gaacatctga ttgaaatccg aggaatcggg atcattgatg
     5221 taatgaatct ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt
     5281 atctggaagc atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg
     5341 ttgaaatcgc ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg
     5401 ttgcaatcat cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg
     5461 caacgaaaac atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact
     5521 agcaaggagt gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat
     5581 tcctatctac tggtatgcat tgatcatcgt atcaggtatc attatcgcta tgtggcttag
     5641 cagcagagag gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg
     5701 gggattgcct atctccatta ttggcgctcg cttatattat gttttatttg atcttcccca
     5761 gtatatagct gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg
     5821 cggactgatt gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt
     5881 gtggactttt ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg
     5941 gggaaatttc atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag
     6001 tctgcattta ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc
     6061 aacatttctt tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg
     6121 aaagaaacca caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag
     6181 tttcgggcgt ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat
     6241 ccgtatttct caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg
     6301 gcgcagaaaa aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat
     6361 tatctgattt ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata
     6421 gagtagcggt tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa
     6481 acggacatga cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc
     6541 aacacacgaa tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca
     6601 ccaatatgga agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag
     6661 caatacgttc tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc
     6721 atgcaagtaa aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg
     6781 aaatcccagc agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag
     6841 aagtagcaaa acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa
     6901 aatatgtaca agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga
     6961 tcggtgtaga gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc
     7021 acggcttagg gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga
     7081 tcagccgttt aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggaa
     7141 taggcgatct agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact
     7201 tgttaggaca agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag
     7261 gtgtgaatac tacccaagca gcgatggaac tagcccaaat gatggaaatc gaaatgccca
     7321 tcactcaaac aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag
     7381 aaatcatgtt acgtgatgga aaaaaagaaa atgaattctc attaacaatt ttttgaagga
     7441 gcgaatgtat atgaaagtta aaaaagcagt tattccagca gcaggattag gaacaagatt
     7501 tttaccagca acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat
     7561 ccagttcatc gttgaagaag cgttgaaatc aggtatcgaa gacatcttga tcgttacagg
     7621 aaaagctaaa cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct
     7681 aagtgaaaaa ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt
     7741 gcatttcatc cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc
     7801 ctttattgga aatgaaccat ttgtcgttat gctaggcgat gatataatgg aagacgaagt
     7861 accattgaca cagcaattga tcgatgacta tgagcgtaca cacgcttcta cgattgccgt
     7921 tatgcaagtt cctcacgatg aaacttcaaa atacggaatc atcaacccag gggaagtact
     7981 ggaagatggg ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc
     8041 aagtgattta gctattattg ggcgttactt attgactcca gaaatctttg atgtattatc
     8101 agaacaacaa ccaggtgctg gaaatgagat ccaactaact gacgcaatcg atacgttgaa
     8161 taaaacacaa cgcgtatttg cccgtaaatt cacaggtaaa cgttatgacg ttggtgacaa
     8221 atttggtttt atgaaaacaa gtattgaata tggtctgaca catccagaag taggggcacc
     8281 tttgcgtgaa tacatcatgt ctttaggtaa aacattagaa gcagaaggaa gcaagatcaa
     8341 tcataatcca aaaggaataa cagaaatcaa agaagaaaag aaaaaataat ctttttttct
     8401 aacgagcttc agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa
     8461 aaacacgaaa atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag
     8521 gaggagaaat tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gttttcatcg
     8581 ttcgcgtatt gttgcaagtt tccaaaactt tagaaaaagt agaccagact gtcgcagaag
     8641 caaatacgac aattgagatc gtcacacaag acgtagatct tttatctaga caagtagaag
     8701 gcttgctagt caaaagtaat gagctgctct tggatatcaa tggaaaagtc gcaacgatcg
     8761 atccattgtt cacagcagtt gctgatttaa gtgaaagtgt atcggaactt aatcattcta
     8821 gtaaaaacat cgtgacgaaa gtcggcggaa tcggaaaaac gacagctaaa gcgactgttg
     8881 ccggaaaagt cggtggaaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga
     8941 caggaggaaa ataagaatgg ctaaaaaagg tggattttta ttaggtgcag tagttggagg
     9001 tacagccgca gcaatcgccg cattattgtt agcacccaaa tctggtaaag aacttcggga
     9061 tgaattggct gctcaagcag atgatttgat ggacatggct tcagattaca cagatatggc
     9121 aaaagaaaaa tcatctgagt taacaggtat tgcaaaagaa aaagcgcaag gtctttctca
     9181 acaagcaggg gatctagcag gcaatgttaa gcagaaggta agtgaatcag cagaggaagc
     9241 aggaagtgaa tcagatgata tcttgttaaa cctcaaagaa caatcagctg atttatctaa
     9301 tcgtttcaaa aaaacagcag aagatctgaa aaagccagca gacgaattag gtagtattgc
     9361 taaagatact tcagaagata tcatgatcga tgtaaaagat gctgcagata aagcaggaga
     9421 gacggtcaat gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgtt
     9481 gaaaatggca aaagaagatg caaaagaagc taaaaaagaa atgaaagata caatgacaga
     9541 ttcgactacc aatcactaag attactcatt tctagaataa aaaaggtgag agcggaccag
     9601 ctctcacctt ttttattttc attactctaa aacaagtaat tcttttggtg tagtcgtgaa
     9661 tggcacacaa ccatcacttg tcacatacac acaatcttcg atacgtaccc ctactttttc
     9721 aggaatatag attcctggtt caagagagaa gcacatccct tcttcaatca ctaagtcgtt
     9781 tccttctaca agagaaggga attcgtggac ggttgttccg attccgtgac ctaaacggtg
     9841 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
     9901 acctgctgtc acacctgggc gaactgcctc ttgtgcggca agctgtgctt caagagtgat
     9961 gttatagatt ttttcttgga actcagttgg tttttgataa gcaactgtac gagtagcatc
    10021 actgcaatag ccattccaaa taacgccaag atcgaataac actagttcat ttggtgaaac
    10081 cttcgtgctt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    10141 gtcgaaagac atttgtgaaa caccttgttt ttttaattgg tattcgattt ctgcgataat
    10201 tgcttgttca gttacgcctt cttttactgt tttgaagcca atttcaaagg caacatcggc
    10261 ccatttacca gcttcgatca gtttgtcttt ttcttctacc gttttgatca gttgcagacg
    10321 ctggataata gaagttagat cggtgtaaga atctgtacct gtcaagatat cagagatagc
    10381 atcgaagcga tcgacagtca gagcattctt ttcaatacct acattaccag gttgcccata
    10441 gcgtgcgcga agctcttttc cgatgatctc ccaaggattt tcgctgtcta agtagccacg
    10501 tacatcgtac gcccagctgc tgttttgagc atcttcaact tccagagcag gagtgaaaag
    10561 gaaaggatct tgttccagag ggataaaaag agctagtaca cgctcgtgag gatcactttc
    10621 atatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt ctacttggtg
    10681 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    10741 ctaaattaat tttgtattca ataattgtac cactgattga ttaaaagacc aacgttctta
    10801 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attgttgaga
    10861 ttatttgata tcctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    10921 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    10981 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    11041 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaat gcagtcgcac
    11101 gtggcttggc aagtaagaaa acaacaacag ttggggtaat cattcctgat gtcagcaata
    11161 tgttctttgc ctctttagca cgtggtatcg acgatgtggc aacaatgtac aaatacaata
    11221 tcatcttagc aaactcagat ggcaacgatc aaaaagaagt caatgtattg aataatttat
    11281 tggctaagca agtggacggc gtgatcttta tgggtcacca tatcacagac gaaatccgtg
    11341 gagaattttc acgttcaaaa acgcctgttg tactagctgg ttccattgat ccagatgaac
    11401 aagtaggtag cgtaaacatt gattatgtgt cagcaacaaa agatgcagta aataaattag
    11461 caaaaagcgg caataaaaaa atcgcttttg tcagcggtgc gctgattgat cctatcaatg
    11521 gtcaaaaccg tctaaaaggc tataaagaag cattaagcga aaacaatcta ccatatagcg
    11581 aaggattgat ttttgaggca caatacaact ttaaagatgg tctgtctcta gttgaccgga
    11641 tccataatag cggtgcaaca gctgtctatg tttcagacga tgaattagcc attggtatct
    11701 tggatggttt attagaccgc ggcgtaaaag taccagaaga attcgaaatc atcacaagca
    11761 acaattcttt actgacagaa gtagctcgtc ctcgtttgac aagtatcaca caaccattat
    11821 acgatttagg agcggtatcc atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    11881 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatcaact aaataataaa
    11941 agatcaatca tttaaaggtt gttccaaaag tggtcaactt ctggaacaac gtttatacga
    12001 gaaagggggt tatgacaaaa ttttgtcata accccttttt ttttgtgact ttaaaaaaag
    12061 agaaaatggc ggaaacaaac cttacgtcca tcgtatacga cacaatctgt gcgtagatgc
    12121 tacattttgt tcactactat caaaattact ttaaaatgaa aacgtgataa aaaagaaagt
    12181 agaaaagtag ataagtaact ctaaaaaatc caaaaaaatc caggctcgat tgcgtactta
    12241 tcttaaagga gaaacaatcc atcatatata acccgaaagg agacttctat ttgcctaaga
    12301 aaagacgaaa gaccatagtc atcattttga ccattcttac tataggaatc ggagggaaat
    12361 tttacatgga caaaagagaa atgcaaagac agaaagattt actcgcagta gaaaaacagt
    12421 ccgtcaaagt tctgaaaaat acctttgcgg atatcaagga agtgaagatt gaaaaatctg
    12481 ctcgaaatga aatgacgggt tcataccgga tagttattct aatgactaat aaacaagatc
    12541 aatcgattta tttttcttat agtttttgga aagaaagaaa tgagatagga agttacggaa
    12601 tagttgatga aaaaaagcaa aaagaaggaa atacgctcaa taaagttaaa gtaacttact
    12661 ctaatggaaa tgaggaaagt atttaatgat tactgacaaa caatatagtg aattaagtga
    12721 tgctgtttac tggctagatc ctaaacatag agactatgta cctgaaatgc aagaaaattt
    12781 aagttttaaa attaacggca ctatgtataa aatcctaaaa atcaaaaact ccttcgacgg
    12841 catgcaggcg atggctgtag cgccaatcgt ccatagcaaa ttggagaaaa atttcaaaaa
    12901 taaaaaaatt cctgcaaact ttcgcgtact taaataatga aggtgtcaat acttacataa
    12961 aaagaaagga ggcacgttct tgtcaaggaa aataagaaaa ctcagcttag ccattttatt
    13021 aatcgtcata ttagggatcg gagggaaagt ctatatggac aaaagagaag cacaaaggca
    13081 gaaagattta ctcgcagtag aaaaacagtc agtcaaagtt ctgaaaaata cctttgcgga
    13141 tatcagggaa gtgaaggtag aagaatttaa aaaaaaccct gttaccggaa gttatggtgc
    13201 acttgtgaca atgacaaata atgaagggaa atctgtttat tttgattata gtttttggaa
    13261 ggagagaaat gaacttggtg gttttggcat tgagaacaga gcggtacaaa agacaggagt
    13321 cactataaaa agagtaaaag taattttttc taacggacag gaggagttag tatgacaata
    13381 aacgataagc attacaatga tattagtgag aaggtttatt ggctagatcc caaatatccc
    13441 cgctacaatg agggatacaa aaagaactct gttaaagaat ttgctggtat ggaatttcaa
    13501 atcctacaaa tcaaagactc attggacggc atgcaggcga tgtctgtagc gccaatcaag
    13561 aacggtaaag tggatctttc tcaagtagtg attgcttttg caggaacgaa cttaagtgat
    13621 tggaaagatt tggaaacgga tgcaagaagt atcatgaatc tctacgggaa tagaccagga
    13681 gcaagttttt ctgaaaaagc aacaacaaac aagattttat ccgttagtgg attgaaaaca
    13741 gcggggcaaa tcaattcagc gattgcattt gctaatgaag taaaaaagga atatggagaa
    13801 gctgctattt ctactaccgg ccattcatta ggtggattct tagcattata tgtagcagca
    13861 gagaaacaat ggaaaaatgt cggcttcaac gggccggacc cgtatgatct cttatcacca
    13921 gaagcaaaaa aatgggtgga agaaaatccc ggtatgctca ccaattaccg aaatcacgcc
    13981 gattttatcg gtaattttgg tggtaatggc acaggtgctg aagttcggat cagtatggaa
    14041 acagcattct tgaatctgct tgcttcccac agtttaacct cttggaagtt tgatgaaaat
    14101 ggaaagctga ttattcctga aaatgaaaac aatgttaaag ctcgaaaaca acagaaagaa
    14161 aaccaagcga cccagttgtt ttcctatcag ttgcaagaac tgaacaacat acgtaaaaaa
    14221 ctacgattaa gtggcggccg gctgacagtt aatgaacaaa tttacttaga tgacacacaa
    14281 gctaggttag tagtggaaaa gttatcggca aaaatgaaaa gcgctatgga agaagttatc
    14341 cgcatcaatc aaagaggaat ttttgagcta gagaaaaaat ggcaagaagg cttgcggcaa
    14401 gcacgtgttg tagccccttc gttgactgaa caggaaatca tagaagcatt agctagtgtg
    14461 ggcgccacaa aagaaaactt agtcgattct gcgaaagaag tctatctgct acgaatcaat
    14521 aaggcaagac gtatggggga ggcctttgat acattggcga aggagatcca agacaaaatc
    14581 aatgaaatcg tggcacgaga tcgagaatta gcacagcaat tcaattaaag gagaagtaga
    14641 tgaaactaga agaattggaa agagattatc gaagtgaacg aagacaatta gaagctcaag
    14701 aagatgaatt gatccacttt aagaatcaag gattacgatt gatggagcaa gaggagcagc
    14761 gactgcatca tgtattaaag aacttagctt tagataatga gcctctgttt caagcaagac
    14821 gaagttattc gaattatcag gagcattatc aacaagccct acaagtagaa cgacgaaaac
    14881 tctatcagca gcaagatgaa ctagaacgtt cctatcgtca aaaaatcaga caaataacta
    14941 tggagaaatc atcttatgaa tgaattttta atgcaattac taaaagaatt agagaaaaat
    15001 ctacatcgat caaatgtacg ttcagttggt atggggatat ctcagcaaat ttgggcagaa
    15061 aaacgattga cagatcaaaa ctaccaaatg actgcagaag aagctgctca agaattttct
    15121 aaccgacatg tacacttgaa aggatcagta aaagggcaat ttgcagaaaa actaactgaa
    15181 ttattccaaa gacaagaaaa gaaattaaaa cagtttcgtt agtaaagtta tatagaaaaa
    15241 ttttgtgaaa at
//
LOCUS       DAZKCE010000069        14681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000069 DAZKCE010000000
VERSION     DAZKCE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.070"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            429..962
                     /locus_tag="ACG8KQ_001926"
     CDS             429..962
                     /locus_tag="ACG8KQ_001926"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9104849.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1452..1976)
                     /locus_tag="ACG8KQ_001927"
     CDS             complement(1452..1976)
                     /locus_tag="ACG8KQ_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9104850.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1969..2442)
                     /gene="tsaE"
                     /locus_tag="ACG8KQ_001928"
     CDS             complement(1969..2442)
                     /gene="tsaE"
                     /locus_tag="ACG8KQ_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9104851.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2565..3545)
                     /gene="pta"
                     /locus_tag="ACG8KQ_001929"
     CDS             complement(2565..3545)
                     /gene="pta"
                     /locus_tag="ACG8KQ_001929"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9104852.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3698..4378)
                     /locus_tag="ACG8KQ_001930"
     CDS             complement(3698..4378)
                     /locus_tag="ACG8KQ_001930"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9104853.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4504..5373
                     /locus_tag="ACG8KQ_001931"
     CDS             4504..5373
                     /locus_tag="ACG8KQ_001931"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9104854.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5399..5914
                     /locus_tag="ACG8KQ_001932"
     CDS             5399..5914
                     /locus_tag="ACG8KQ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9104855.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5961..6524)
                     /locus_tag="ACG8KQ_001933"
     CDS             complement(5961..6524)
                     /locus_tag="ACG8KQ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104856.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6579..6758)
                     /locus_tag="ACG8KQ_001934"
     CDS             complement(6579..6758)
                     /locus_tag="ACG8KQ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104857.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6835..7344)
                     /locus_tag="ACG8KQ_001935"
     CDS             complement(6835..7344)
                     /locus_tag="ACG8KQ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104858.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7440..7820)
                     /locus_tag="ACG8KQ_001936"
     CDS             complement(7440..7820)
                     /locus_tag="ACG8KQ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9104859.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8150..8899
                     /gene="pnuC"
                     /locus_tag="ACG8KQ_001937"
     CDS             8150..8899
                     /gene="pnuC"
                     /locus_tag="ACG8KQ_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9104860.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9220..10437)
                     /locus_tag="ACG8KQ_001938"
     CDS             complement(9220..10437)
                     /locus_tag="ACG8KQ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9104861.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(10695..11972)
                     /locus_tag="ACG8KQ_001939"
     CDS             complement(10695..11972)
                     /locus_tag="ACG8KQ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104862.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(12051..12695)
                     /gene="pcp"
                     /locus_tag="ACG8KQ_001940"
     CDS             complement(12051..12695)
                     /gene="pcp"
                     /locus_tag="ACG8KQ_001940"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9104863.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(12894..13838)
                     /locus_tag="ACG8KQ_001941"
     CDS             complement(12894..13838)
                     /locus_tag="ACG8KQ_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9104864.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(13840..14526)
                     /locus_tag="ACG8KQ_001942"
     CDS             complement(13840..14526)
                     /locus_tag="ACG8KQ_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9104865.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4217 a         3026 c         2657 g         4781 t
ORIGIN      
        1 tttcgtttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag
       61 ttgtgaaatc agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat
      121 attttgctat tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga
      181 agttgtgaaa aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca
      241 caactttgca taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc
      301 acaaggaatg agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt
      361 tttttgttaa atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt
      421 ttacagaaat gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg
      481 cttgttctct tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat
      541 tgatacagcc tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag
      601 aagacgtacg caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc
      661 aaaaaaatcg tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat
      721 gcttagatta ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta
      781 gccgtcgcct gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga
      841 acatccaatt agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc
      901 tgctttatca agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt
      961 aaaagaatac ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac
     1021 aaggagcaat ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag
     1081 gagctggcag gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt
     1141 aataagaaca gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc
     1201 gcagatgcaa gcttttggac accgtttacc aagaggttgt gaaatcagcg ttttgacctg
     1261 agtattagag aaacaaacgg caaaatagtt cctcatattt tgccgttttt tgagctaata
     1321 ccgaaggtca gctgattgaa caccgtttat tcaaaaaaaa tggactgcga cacgactttt
     1381 gtcacagtcc cttcgtgtgt ttttctgcct tctcaaaagg atgacagttt ctcactcttt
     1441 tcctatttct tctattcaat caaataactc attttatata catctaaaaa ttcacctaga
     1501 tctgttcttg cccctctagg catgaccgct tcgatttgga agcccatttt tcggtaaagg
     1561 tgtacggctc tttcattacg aacttgaacg tcaagctcca atcgaaaaag tacacccatt
     1621 tccttcgccc aactgatgat ttcttctagc ataagagtgc ctagtcccat tccccaatat
     1681 tcctgcagaa tactgatacc gacttctccc acgtgagaca aacgaaattg actgtctgcc
     1741 ttgacagatg ctgttccaat aatcgtccct tcatagattg caagcagcaa taaattatta
     1801 ttgctttcgt atatataatc caactcttct tctaaggttt ctggtggtaa caggagttct
     1861 gcttcatcca aaactaaaaa ttctgtctcc ttattaactt gtcccatcat caaaagaatg
     1921 tcttttgcat ctcctggcac tgcttcccgc aagatgatcc caacttcttc actcatgatt
     1981 ttcagcccat ttttctagaa ttcgctgttt gaaggtttca ctttgctccc cgtatgagat
     2041 tagtttcaca taccgctttt ctaaatctgt atcacttttt tctaaaatca gttccaaata
     2101 atcctccggc agtgcttctt ctaatagatt tccccattca acaacggaaa gcccatcacc
     2161 ttcaaaatat tcatctaacc ctaaatctgc tccgctagct gctacccgat aaatatccat
     2221 atgatacaac ggaatccgac cttgactata ttctctaata atcgtgtatg tcggactttt
     2281 gatcatttgc gttatcccta atccttgggc aattcctttc gtcaacgttg ttttacccgc
     2341 acctagatca ccagtcaata tcagattgtc acctggcatt gcggaatgac caattgcctc
     2401 tgcgaattgt tctgtcattg ttaatcctga tagttcgatc atttttcatc ttctcccgct
     2461 cttctttctt tttgccagta tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag
     2521 tcaagaacag cagctgctgt ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt
     2581 tgtgctgctg tgataatcga taatttatat acatcttctt catttgctcc acgagacaaa
     2641 tcagaaaccg gtttgttcaa gccttgtaga attggtccga ttgcttcaaa gttaccgaaa
     2701 cgctgagcaa ttttatagcc gatatttcct gactgcaatt ctggaaatac aaagactgtt
     2761 gcttgaccag ctacttctga atctggcgct ttcagttgtg caactgaagg aacataagaa
     2821 gcatcaaatt gcaattcgcc gtcgatcatg tattgtggcg ctagtttttt agcgatttta
     2881 gttgcttcaa ctactttgtc tacttctggc gatttcgctg agccttttgt cgagaagctc
     2941 atcattgcta cttttggttc gatatcgaac aattctgctg tttttgcaga atctacggcg
     3001 atttcagcca attcttgcgc agttggattc acgttgatcg cacaatccga aaagacatat
     3061 ttttcttgat ctcttccacg taccatgatc atcgctccgc ttgtccgact gatgccaggc
     3121 tttgttttga tgatctgaag tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca
     3181 gagaccattc cgtctgcaat ccccatatac gtaagcatcg taccaaagta attcacatct
     3241 tttaaaatct tttctgcttg ttctttggat gcctttccat ttctacgttc aacaaaagta
     3301 tccaccattt catcccactt atcgtaatta tcagggctga tgatcgtaaa gctagatgtt
     3361 ttgattcctc tggcatgcgc tgcatttctt atttcttctt cgttccctac taaaatgggt
     3421 tccacaagtt cttctgcttt caaccgggca gctgcgccta aaatacgagc atcagtagct
     3481 tccgggaaaa cgattttgat cccacggcga acaattttaa atcttaaact atcaaacaat
     3541 tccacaagat gacctccaga cgtttttata tgtattatca tacactattc cagaaaaaaa
     3601 gaacagttta ttttatctgt atcagtcaaa aatgctggaa aaattaagtt tttcacatac
     3661 aaagcgaact gcactataac aggaaagcgc tgttttatta cactgtttca ggcagttgcc
     3721 aatcaatcgg ttcttctcct aacgcaagga gcgcatcatt cgttttcgag aagggtttgg
     3781 aaccaaaaaa gccacgactt gcagataacg gactgggatg tggagatgtt atgatgatat
     3841 ggcgactctt atcgatcatt ttgatttttt cttgtgccgg ttttccccac aagataaaca
     3901 cgattggttt ttcccgttca tttaattttt caatgatcgt gtctgttaat ctttcccaac
     3961 ctttcccgcg atgagaatac gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa
     4021 ggactccctg tttagcccat gacactagat ttccatgttg aacaggtgcg attccaagat
     4081 catcataaag ctctttatag atatttctta acgatggcgg gatctttacg ccaggttgca
     4141 cagaaaaaga taagccatga gcttgatttt ctccatgata aggatcttgt cctaaaatca
     4201 ccactttaac ttcttcataa ggagtcaatt ccaatgcttc atagatatga tacatatctg
     4261 gatggatctt ttgggacgca tattcttttt ttaaaaattc tcttagatgc agataataat
     4321 cttttgaaaa ttctgaggac aaaacttctt gccaactgtt atgaatgatt tctttcaaaa
     4381 actccacctc ctaataaaaa gaataactca cattttcttc aatcacaagg gacaatcttc
     4441 accctcagca cttgattaat atggtaaact aggaacaaga agcagaaagg aacgataatc
     4501 attatgatca aattaattgc atcagatatg gacggcacat tattagatgc ccatatgagt
     4561 gtttctcctg aaaatgccga ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg
     4621 gtcgctactg gtcgaaacta tcaagaagct cgcgcagctt tagatgaggt aggaatcgat
     4681 tgtgcgatga tcactttgaa cggggcacag gtatttgata aagaaggaaa ttcattgttt
     4741 accgtgtcga ttccaaataa tcaagcaatc tctgttttag atattctaga tgctaacggt
     4801 atttactatg aagtagcaac caacgatggt ctttattctg aaaatcaagc gaaacgaatt
     4861 gaaagttttg cttccatggt ggctacccat ttacctcacc ttacgtataa aatggcgatt
     4921 gccatggcct cagctaagct ggagttattg cacatcacat acgtggacag tattcgtgct
     4981 attttggaag aaaagaatct ggaagtttta aaaattattt gttttcatac agaagggcca
     5041 gctgtattag gtccagttgg aaagcagatt tctcttttag gcgatctagc cgttacttcc
     5101 tctggtcaaa acaatatcga agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc
     5161 catgtcgctc acgagcgtgg gatttcttta gaagaagtca tgacgattgg cgataacttc
     5221 aatgatgtca gcatgctgca agtagcagga gtcagtttcg caatgggaaa tgcagaaatc
     5281 gaagtaaaag attatgccaa atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa
     5341 gccatattgc gcgcgattgc agaagacttg tagttttatc tagaaaagag ggatatagat
     5401 gtcagaattt ttcattcagg aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa
     5461 cgaaaaagga cattccctgt ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt
     5521 gtctctttat gccatgaatg gcgatctcgt tgctcacatc aagcagataa gcctgacatt
     5581 cggtacgcgt tttgagctgt ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt
     5641 caattggccc ggtgactttt attatattcg tagacttcac tggacagtcc aaggtgatat
     5701 ctataatcat caatatcaaa tccatcattt caatcagcaa atcatgaaaa tggataaagc
     5761 aacacttttt tcaggagatt actatgtatt ggatgtctca aatgatgaag atgctccagt
     5821 ttgtatttgt attgctgcgg ttttagatta ctggctctac cgtaaagaca aaagtcgcga
     5881 taagcctgtt tcttaccgac ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata
     5941 cgcatttcag gtacatcttt ttagatattc ttttgcatat gatcataaag atgaccactg
     6001 atcatccgct gaccgacaac tttgaagtca tggcgttcgt ataatttttt agcctttgga
     6061 tttttttcat ccacacttag accgatgacg gagcgattag ctttcttcgc caatttaggt
     6121 aatgcttcta ataatcgcga accgatgcct tgtcctctaa aatcttctga tacactgata
     6181 gagtccaaat accattcgtt gggaaacgtt tctttatcaa tgaacaaacg tgtagacgca
     6241 tctagctgat atttctctaa gataggtacc agtggctgat cgatgattga ttcttcttca
     6301 tctgtataac cgtaagcaac tcctgcaact ttaccgtcta tttcttctac gatacctcgt
     6361 gaataactgt aacgataatt tggatcagtg attgcttctt ctaaaatctc taaagtcttt
     6421 tgttcccctg tctgcttcaa aaatggcagt tccatatctt ttagaatcac taagatcaat
     6481 ggggcgattt ctggagcatc ttcttttctc gcaaaacgaa tcatagtttc ctctcctttt
     6541 ctccattcta acatgcagaa aaagaaatcg caaaatcgtt attttaaaaa atcttcaatc
     6601 ttttcagtaa tgaaataacc atgagtaacg attcttgccg cttcagttcg gcaataaggg
     6661 cagacgactg gcatttcttt atcattatgt tcgactacat tattgacttc ttcgtatgca
     6721 aatttttttc cgcaattagg gcatttcatt ttttccatgt tatctcaact tcctttcatc
     6781 tttactctct tagcttactc ttatattgta aaaaagagaa gcaatatcct tcttctatgc
     6841 agatatccat acctgacggt cctgttctcc atctattaat cggactttat aataaggacg
     6901 ttcatctttg actagaagtg tccactcttt cacttttgct tttttcccgt agtagctgac
     6961 tgcttccttc atcgctgctt gcggttgttt gaactcttcc gttttttcta tatcaaaaag
     7021 atgttctgcg gccttttccg tgtcatttcg tacggttgtt ttcccagtgg atggattgac
     7081 tgctatgact tgtccttcac aaaaaaactg gtattcatat gttgcattcg taggattctc
     7141 caaatctttt tcaactgcaa aatgaatagc agatatgtta tgttcttttg tttcgttttt
     7201 tacgattgct gctgcttttt tcaatgagat ccgatattcc tggtcactaa tatcatccat
     7261 gtgaaaagcg gcattgagca gtcccgtcat accggtaatc cctattacga tgagggcagc
     7321 aacaccaaac atcatttttt tcattcttca ttctcctgtt tctttttttt aaagaatatc
     7381 aagaaatgaa cgaatgaaaa atcgaatgaa acgaaaaaaa agtgacaatt ttgttatctt
     7441 tattcgtgca gttctaatgg caatccgtct ggatcaaaga aaaacgtcat tttttttcct
     7501 gtaaaggtat cttctctaat cggttctgat tcgatcccta aggactgcaa ttctgcaatt
     7561 gtttcttcaa tatcgtctac tttgaaagca agatgacgca agccacaagc ttctggatag
     7621 tttggtcgtt gtggcgcatt agcaattcca aaaatctcca attcagcact tcctagtttc
     7681 agatctaatt tatagtcatc tcgttctttg cggtaatttt ctctgattac ttcaaatcct
     7741 aatttattaa cataaaaatc acgtgatttc tgataatctg aaacaatgat ggctatatga
     7801 tggatcgttg taaggttcat atgagcactc ctattcttcg tttaagctcg gatctatatt
     7861 aatctccagt tccattgtaa atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat
     7921 aagaaaaaag gcaaaaaaaa cagaaagtag gtcctccaag acactcttcc tgttcccaat
     7981 ataataaatg aaagtaagaa attatatttt caagaaaaca aacagaaaat tatcataaca
     8041 caaattattt ataattgaaa gactagtttt ttttcaagaa aagcatacaa tagattgtgt
     8101 tagtaaatac agaacacact acatttagtg tcttaaggag tcctcataca tgaagtcata
     8161 ttttcagttt ttagctcatc agctaaaagg ctggccgcaa caaaattatt atcttttctt
     8221 tttcagtctt ggctgccagg tcatgactct tgtcaatagt cccatcactt ggctgtccgt
     8281 tattacattt atcggtacca cgcttggtgt attgtgtatt ttatccatca atgcggcaaa
     8341 atcagtcaac ggtattttag gtattttgtc tgcgatttgt tttattatcg ttgggttttc
     8401 agcaaagaac tatttaagta tcggtgaaca gattgcttat atgatcacat tggatatccc
     8461 cgtattgtta agtaaagaat ggaatcataa tatggcttca aaaatccgaa aattcactgg
     8521 aaaaagctgg tttatcgcaa tcatcagtac tttccttgtc tatcttgttt caggattcct
     8581 gattggtcgt tatacagatg atcctcggcc gtgggtagat gccattgctt tttctattag
     8641 cttgactgct ggtatcatca gtttcttgcg ttacaacaac caatattact ggtggttagc
     8701 ttcagggctt gctcaaatgg tcctttggta tatttcattt acgcatggat ctgcaagttt
     8761 ggcgatgttc gtgaacagct ctgtttactt gctgaatgat gtccttgcat ttaccgtttc
     8821 accttggtat aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag aatactacaa
     8881 cgcacaacaa cacgcataaa aaaactgagc gtgggacaaa agtaaagaac acttttgtct
     8941 cacgctctaa acacgtataa acggcggaat cagaagcaac tccttcggaa ataagctgaa
     9001 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     9061 cttctgtccc agcctctatt tcctacgatt agtcaagtaa aaggtgcaag aaaaatagct
     9121 cgtaaacgaa agcgacaaaa tcaaagcaca ccaaataaag ccccgttcgt aaaaaatttt
     9181 cgatctgggc aaagaagtgg tatactgtaa gtggtttagt taatggtcca ctgagaccgc
     9241 gtacgcatag tcgtactctg tatgtgccct aaccatagcg tgcatacatt tcagaagctt
     9301 gttcatacaa gcaactgtgg caaccttatc tttcttaggg ataggttgct tttttaattt
     9361 gtagtaataa tcgacaatat gattaggagc tgcgcgttgc tggcgaatca tatttcgaat
     9421 ggtgaagaaa ataattttcc gtgctttggg attaccacgc ttattgatgc ggtcttggcc
     9481 agtatactta cctgaatgat accgtcggat gtcgattccg acaaaggcat tcaattgttt
     9541 atgggtttta aatcgtgtga tatctcctaa ttcaccgagc aataaagcgg ctgtgagtgc
     9601 gccaatccca gggaacgttt ggtacaacaa aaagcaagga gagccttcgg cttttttgat
     9661 catttgtgtc gccagtgctt ttttttgttc gagtaaatct tggagaagtt cggcgtaata
     9721 gatcgttttt tgacagtgga tactgtcttt ttcaacagct ggataggcac actgtgcata
     9781 gtggatgatt tgatctgcct tttgctcggc acgattcgcg gagatttttt tccgtgtttc
     9841 gttgatcagt ttatttttta tttttgtccg agtagatgcc aatacaaaat ctggatgggg
     9901 aaaaagacgg atcaatgtca atgcgtaagg ggtcagcctg tttgaaaaga actgttcaag
     9961 ctctggaaaa gtcaattgaa ggcagttgtg taaatccatg cgcagacgag tgattttctt
    10021 ctcaatttct tgataaaaac gggctaaatc gcgcatctct aagtaaaaat tgctttgtac
    10081 ttcttttggc tgacgttgat ttttaagatg agtctgtgcg agccggtggg catccgattt
    10141 atctgttttc caactgcgta aggtgctttc ttccatctgt tttttcgcct ctaacgggtt
    10201 taacaaacag taagaaaagt gatgatcttg aaagaacttt tcgactggtc tggaataaat
    10261 ccccgttgct tcaaaaacaa tcaatggacg ctcaggcaag tgctggatct cttcgagtaa
    10321 tgcatgaaat cctctttgag tgtgttcaat ctcgtcttca aataagcagg tgtcgtcttt
    10381 gtataggaca ctgtagctgt gtcccattga aacatctaat gcaagaatgt aaggcatttg
    10441 ttttcctcct ttttaagttt tgaaagaact ttactgcttc atttcgattc caatttccta
    10501 tacacgggct catggcccca acatacgtaa acctgattct aaaatgacag taaagaagtt
    10561 cggtttttga tagggactcg tagtcctcaa ggctttgttc gaactttctt tcactcttca
    10621 ctataatcta aaaatagaaa atagaggaga acatcattcg tcaatgatgt tcccctctaa
    10681 tcttagtatg ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc
    10741 agtgattctt ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga
    10801 taatgttttc ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc
    10861 actactagtt gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata
    10921 gcttctcgtt ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg
    10981 tcgaagaacc cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt
    11041 tttggttgga tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt
    11101 gttcgttgac gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga
    11161 tgtactggca cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag
    11221 ttgatcaaga attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct
    11281 ttttttatct gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca
    11341 ttttccaatg cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg
    11401 agccgctgag ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac
    11461 aagacaaaac agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc
    11521 cataataatt cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc
    11581 actgtcggag agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt
    11641 ccggtttttg gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac
    11701 aatttttgct ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc
    11761 caatctgttg cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg
    11821 tgcttatcga ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga
    11881 ctgcttgtgt attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac
    11941 cattcttcaa agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa
    12001 aagctggcta ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt
    12061 gttccggcat ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc
    12121 aaagaagaag caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact
    12181 tgatcgggtg agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg
    12241 atcaaatgaa gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag
    12301 atgtacgctg gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag
    12361 gtaaagtaag cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca
    12421 ttatcaggga ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac
    12481 cgcccacctg cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct
    12541 ttgactactt gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca
    12601 ccgctgattt catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca
    12661 ccaaatggat caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt
    12721 taacatatgg ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa
    12781 tagtttaaat aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac
    12841 tacaatgtta gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta
    12901 aatagaatca tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt
    12961 ttgatcactc cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta
    13021 gcagccatcg gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt
    13081 aacgctggat tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg
    13141 gtgatggccg caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa
    13201 atgatgatgc cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc
    13261 actgtcccaa ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac
    13321 atgcttaacg gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt
    13381 cgattatctc gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg
    13441 ccgactaaag cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc
    13501 aaagctggaa taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt
    13561 gatggaagcc ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca
    13621 aagatcaagc caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct
    13681 aagaccgtcc agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt
    13741 cttactccag tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta
    13801 tcgcctaata aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata
    13861 atatttcttc gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc
    13921 cgtgatagcc actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag
    13981 atctttcagt gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc
    14041 aaagaaccag cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt
    14101 aggatggact tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg
    14161 acgaacaaat ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa
    14221 gatcccataa atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga
    14281 agccgctgat tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat
    14341 aaaaatcgcc atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg
    14401 aatgccaaga ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga
    14461 gtctaattta aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt
    14521 ttccatatat tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata
    14581 aaaaatatag ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt
    14641 ttatcacaaa aattatctta cttattgtaa ggtaaagctt t
//
LOCUS       DAZKCE010000070        14672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000070 DAZKCE010000000
VERSION     DAZKCE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.071"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG8KQ_001943"
     CDS             complement(11..1942)
                     /locus_tag="ACG8KQ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9104866.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8KQ_001944"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8KQ_001944"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9104867.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG8KQ_001945"
     CDS             complement(2871..3623)
                     /locus_tag="ACG8KQ_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9104868.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG8KQ_001946"
     CDS             complement(3782..3991)
                     /locus_tag="ACG8KQ_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104869.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG8KQ_001947"
     CDS             complement(4002..5330)
                     /locus_tag="ACG8KQ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104870.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG8KQ_001948"
     CDS             5466..5621
                     /locus_tag="ACG8KQ_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9104871.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8KQ_001949"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8KQ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9104872.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8KQ_001950"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8KQ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9104873.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8KQ_001951"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8KQ_001951"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9104874.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDTLNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG8KQ_001952"
     CDS             complement(9305..10237)
                     /locus_tag="ACG8KQ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104875.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG8KQ_001953"
     CDS             complement(10394..11053)
                     /locus_tag="ACG8KQ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9104876.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8KQ_001954"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8KQ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9104877.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG8KQ_001955"
     CDS             complement(12094..12384)
                     /locus_tag="ACG8KQ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104878.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8KQ_001956"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8KQ_001956"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9104879.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         4175 a         3104 c         2552 g         4841 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgtatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct ta
//
LOCUS       DAZKCE010000071        14549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000071 DAZKCE010000000
VERSION     DAZKCE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.072"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8KQ_001957"
     CDS             4..816
                     /locus_tag="ACG8KQ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9104880.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8KQ_001958"
     CDS             813..1613
                     /locus_tag="ACG8KQ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9104881.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8KQ_001959"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8KQ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9104882.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8KQ_001960"
     CDS             3110..4819
                     /locus_tag="ACG8KQ_001960"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9104883.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8KQ_001961"
     CDS             5031..9389
                     /locus_tag="ACG8KQ_001961"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9104884.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8KQ_001962"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8KQ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9104885.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8KQ_001963"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8KQ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9104886.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8KQ_001964"
     CDS             11227..11520
                     /locus_tag="ACG8KQ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9104887.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8KQ_001965"
     CDS             11517..11828
                     /locus_tag="ACG8KQ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9104888.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8KQ_001966"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8KQ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9104889.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8KQ_001967"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8KQ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9104890.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DAZKCE010000072        13977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000072 DAZKCE010000000
VERSION     DAZKCE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.073"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8KQ_001968"
     CDS             140..460
                     /locus_tag="ACG8KQ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104891.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8KQ_001969"
     CDS             912..1112
                     /locus_tag="ACG8KQ_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9104892.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8KQ_001970"
     CDS             1259..4048
                     /locus_tag="ACG8KQ_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9104893.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8KQ_001971"
     CDS             4095..4622
                     /locus_tag="ACG8KQ_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9104894.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8KQ_001972"
     CDS             4643..5833
                     /locus_tag="ACG8KQ_001972"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9104895.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8KQ_001973"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8KQ_001973"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9104896.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8KQ_001974"
     CDS             7966..8640
                     /locus_tag="ACG8KQ_001974"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9104897.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8KQ_001975"
     CDS             8637..8978
                     /locus_tag="ACG8KQ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104898.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8KQ_001976"
     CDS             9008..9367
                     /locus_tag="ACG8KQ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9104899.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8KQ_001977"
     CDS             9627..9749
                     /locus_tag="ACG8KQ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104900.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG8KQ_001978"
     CDS             9891..11975
                     /locus_tag="ACG8KQ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9104901.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG8KQ_001979"
     CDS             12286..13752
                     /locus_tag="ACG8KQ_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9104902.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgtttt
//
LOCUS       DAZKCE010000073        13834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000073 DAZKCE010000000
VERSION     DAZKCE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.074"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..364
                     /locus_tag="ACG8KQ_001980"
     CDS             170..364
                     /locus_tag="ACG8KQ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104903.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            633..932
                     /locus_tag="ACG8KQ_001981"
     CDS             633..932
                     /locus_tag="ACG8KQ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104904.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1029..1463)
                     /locus_tag="ACG8KQ_001982"
     CDS             complement(1029..1463)
                     /locus_tag="ACG8KQ_001982"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104905.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1695..2405)
                     /locus_tag="ACG8KQ_001983"
     CDS             complement(1695..2405)
                     /locus_tag="ACG8KQ_001983"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9104906.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2395..3255)
                     /locus_tag="ACG8KQ_001984"
     CDS             complement(2395..3255)
                     /locus_tag="ACG8KQ_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9104907.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3260..3898)
                     /locus_tag="ACG8KQ_001985"
     CDS             complement(3260..3898)
                     /locus_tag="ACG8KQ_001985"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9104908.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3913..4212)
                     /locus_tag="ACG8KQ_001986"
     CDS             complement(3913..4212)
                     /locus_tag="ACG8KQ_001986"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9104909.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4255..5739)
                     /locus_tag="ACG8KQ_001987"
     CDS             complement(4255..5739)
                     /locus_tag="ACG8KQ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9104910.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5759..6220)
                     /locus_tag="ACG8KQ_001988"
     CDS             complement(5759..6220)
                     /locus_tag="ACG8KQ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9104911.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="ACG8KQ_001989"
     CDS             complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="ACG8KQ_001989"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9104912.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7523..8293
                     /locus_tag="ACG8KQ_001990"
     CDS             7523..8293
                     /locus_tag="ACG8KQ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9104913.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8566..9393)
                     /locus_tag="ACG8KQ_001991"
     CDS             complement(8566..9393)
                     /locus_tag="ACG8KQ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9104914.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9961..11196
                     /locus_tag="ACG8KQ_001992"
     CDS             9961..11196
                     /locus_tag="ACG8KQ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9104915.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12195..12455)
                     /locus_tag="ACG8KQ_001993"
     CDS             complement(12195..12455)
                     /locus_tag="ACG8KQ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9104916.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12482..13783)
                     /locus_tag="ACG8KQ_001994"
     CDS             complement(12482..13783)
                     /locus_tag="ACG8KQ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9104917.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4448 a         2832 c         2177 g         4377 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
      541 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
      601 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      661 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      721 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      781 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      841 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      901 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      961 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1021 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1081 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     1141 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     1201 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     1261 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     1321 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     1381 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     1441 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     1501 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1561 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1621 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1681 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1741 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1801 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1861 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1921 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1981 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2041 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     2101 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     2161 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     2221 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     2281 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     2341 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     2401 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     2461 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     2521 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2581 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2641 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2701 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2761 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2821 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2881 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2941 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3001 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3061 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     3121 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     3181 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     3241 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     3301 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     3361 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     3421 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     3481 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3541 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3601 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3661 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3721 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3781 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3841 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3901 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3961 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     4021 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4081 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     4141 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     4201 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     4261 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     4321 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     4381 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     4441 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     4501 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     4561 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4621 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4681 aaacaagacc ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4741 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4801 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4861 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4921 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4981 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     5041 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     5101 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     5161 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     5221 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     5281 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     5341 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     5401 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     5461 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     5521 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5581 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5641 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5701 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5761 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5821 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5881 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5941 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6001 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6061 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     6121 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     6181 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     6241 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     6301 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     6361 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     6421 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     6481 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6541 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6601 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6661 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6721 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6781 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6841 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6901 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6961 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7021 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7081 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     7141 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     7201 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     7261 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     7321 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     7381 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     7441 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     7501 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7561 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7621 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7681 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7741 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7801 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7861 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7921 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7981 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8041 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     8101 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     8161 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     8221 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     8281 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     8341 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     8401 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     8461 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     8521 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8581 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8641 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8701 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8761 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8821 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8881 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8941 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9001 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     9061 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     9121 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     9181 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     9241 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     9301 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     9361 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     9421 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     9481 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     9541 tttctttttg tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt
     9601 catggcgaga aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata
     9661 atgacgacag cacacctatc aattaatata attattttat ttttttatgt tctcatgttt
     9721 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
     9781 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
     9841 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
     9901 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
     9961 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10021 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10081 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    10141 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    10201 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    10261 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    10321 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    10381 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    10441 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    10501 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    10561 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    10621 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    10681 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    10741 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    10801 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    10861 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    10921 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    10981 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11041 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    11101 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    11161 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    11221 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    11281 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    11341 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
    11401 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    11461 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    11521 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
    11581 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    11641 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
    11701 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
    11761 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
    11821 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    11881 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
    11941 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12001 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12061 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    12121 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
    12181 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    12241 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
    12301 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    12361 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    12421 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    12481 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    12541 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    12601 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    12661 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    12721 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    12781 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
    12841 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    12901 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    12961 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13021 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13081 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    13141 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
    13201 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    13261 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    13321 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    13381 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
    13441 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    13501 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    13561 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    13621 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    13681 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    13741 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    13801 aataaaatat gaaccattca ttgcatgaca acgc
//
LOCUS       DAZKCE010000074        13807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000074 DAZKCE010000000
VERSION     DAZKCE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.075"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8KQ_001995"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8KQ_001995"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9104918.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG8KQ_001996"
     CDS             complement(2260..2511)
                     /locus_tag="ACG8KQ_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9104919.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8KQ_001997"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8KQ_001997"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9104920.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG8KQ_001998"
     CDS             3461..3679
                     /locus_tag="ACG8KQ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104921.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG8KQ_001999"
     CDS             complement(3968..4480)
                     /locus_tag="ACG8KQ_001999"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9104922.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8KQ_002000"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8KQ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9104923.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG8KQ_002001"
     CDS             complement(6933..7376)
                     /locus_tag="ACG8KQ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9104924.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG8KQ_002002"
     CDS             complement(7377..8165)
                     /locus_tag="ACG8KQ_002002"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9104925.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8KQ_002003"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8KQ_002003"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9104926.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG8KQ_002004"
     CDS             complement(9280..9867)
                     /locus_tag="ACG8KQ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9104927.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8KQ_002005"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8KQ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9104928.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG8KQ_002006"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG8KQ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9104929.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG8KQ_002007"
     CDS             complement(12784..13719)
                     /locus_tag="ACG8KQ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9104930.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAZKCE010000075        13630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000075 DAZKCE010000000
VERSION     DAZKCE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.076"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..705
                     /locus_tag="ACG8KQ_002008"
     CDS             106..705
                     /locus_tag="ACG8KQ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFP9104931.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
     gene            705..1280
                     /locus_tag="ACG8KQ_002009"
     CDS             705..1280
                     /locus_tag="ACG8KQ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFP9104932.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            1305..4976
                     /gene="brxC"
                     /locus_tag="ACG8KQ_002010"
     CDS             1305..4976
                     /gene="brxC"
                     /locus_tag="ACG8KQ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFP9104933.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            5040..8672
                     /gene="pglX"
                     /locus_tag="ACG8KQ_002011"
     CDS             5040..8672
                     /gene="pglX"
                     /locus_tag="ACG8KQ_002011"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFP9104934.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            8788..11310
                     /gene="pglZ"
                     /locus_tag="ACG8KQ_002012"
     CDS             8788..11310
                     /gene="pglZ"
                     /locus_tag="ACG8KQ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFP9104935.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            11428..13461
                     /gene="brxL"
                     /locus_tag="ACG8KQ_002013"
     CDS             11428..13461
                     /gene="brxL"
                     /locus_tag="ACG8KQ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFP9104936.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         5006 a         1837 c         2642 g         4145 t
ORIGIN      
        1 tataataaat atgtattgta tagtagatac taaaaaatga atgctaacga tgagcaaaaa
       61 acacttgttt acttattgaa ttatagatga ttaggaggca atctattgaa taaaaaatat
      121 tctgctggtc tagtttctaa acgcttttgg ttttcagagt tcaagttata tattcaatta
      181 cgactagaag gatatacaga tatagaaatt aaagagaaaa atgaacatga aaatattttt
      241 tttgcagtga gcagtaaccg tcaaaaagaa atctatcaga acgtgaaaag gagagttagt
      301 caactggatg aagctggatt gcaacttttc aatcagttaa atatagataa tcaaaagtta
      361 gtgaatttga tttccatcct tatgattgat gatcttttta atgaatttat gactgaggta
      421 tatgctgaaa atctacgtaa aggaaatttg caattaacac cagttgatta ccgttctttt
      481 tttacagaga agcggagaag ttcagaagat atttcaaaat ggaaagatta tacgattaaa
      541 cggcttattg ccgcttatca aacgaatcta attgaaacag gattagtcag aggggaaaaa
      601 gaatatggac gaatgactcc gatattatta gataaacgat tgattcaatg gttagttgac
      661 aagaatcgta aagatattgt actagcactg ggaggaattt tataatgtca ttatcgattc
      721 aagagcattt ggaaaagttg aatgaagtaa ttcaaaaaga agatttttta ttgaatcgtg
      781 gtttaggtaa tgaagtaggc tattatatct ttgactacga tccgaaagac gagttaattg
      841 taagagatta tattaatgat atatctggaa catctgttgg tgataattat gaagtgaaag
      901 tttataatct ctatgtgatt atgttagagt atctagaaaa acgcggtttc actgaaaaag
      961 cgattaagat ggaagaacga cgtggtttac aaaaattagt ggaaagcatt ggtcgtttat
     1021 tgaaaatgga tttacctgag gaagaaaatg tattttttga atatgtggat gcccatacac
     1081 cagaaaaatc agttgtgttc ctgacaggaa ttggcgaatt atatccatta attagagcgc
     1141 actctatttt aaataaaatg catcaggtat ttgatcgcgt accagttgtg atgatgtatc
     1201 caggggaata tgatgaatta agtttgacaa ttcttaatac caataaagat gataattatt
     1261 atcgagcatt tagaatttag ttgggatgga aggtaggaga ttaaatgcta attaaagata
     1321 tttttgcaaa accaattgac cgaaatattc aaggagttat taaggtcggt caagcaaagg
     1381 atgaaaatgt tcaacaagaa ttagaagaat acgtggttac gaaagaacta caaaaacact
     1441 ttaaaacaat ttttgatgcc taccaacgga gtatcaatac accaacagat aaaatgggtg
     1501 tttggattca ggggttcttt ggttcaggta aatctcattt cttaaaaatt atttcttact
     1561 tgctcgcaaa tcgagaaata aatgacaaga aagccattga ttacttcaaa gaagatggga
     1621 aaatcaaaga tccactgact ttggcaacga tggaacggtg ctcacaaata aatacagatg
     1681 ttattctgtt taatattgat tcaaaaagtg atgcaggtgg taaaaaagac aaaaacggaa
     1741 tcgtgaatgt ttttttaaag gtatttaacg aaatgcaagg attaagcacg attccacatc
     1801 tagcagattt tgaacgtcgg ttaattaaag aagaaaagtt tgatgcattc aaagctgctt
     1861 ttgaagagat taatggggca aaatggcaag aagagcgcca tgagtttgat tggatccaag
     1921 atgatatcaa agaagcggca cttaaaatcg attttatgag tgaagccgct attgataatt
     1981 ttttagaaaa agcatcagga aattatttaa taagcattga tgactttgca aatctagtta
     2041 aaagctatct tgattccaaa gaagcagacc atcatattgc ctttctagtc gatgaggtcg
     2101 gacaatatat cggcggtaat cgtgagttga ctttgcagtt acaaaccgtt gtggaagatt
     2161 tgggtcgaat ttgtgaaggt aaggcttggg tattagtgac aagtcaggaa gcgattgatc
     2221 acgtaacaaa ctttgataac aattctgata atgatttttc taagatcaag gatcgttttg
     2281 ccacaactgt gagtttatct tcagcaaatg ccgatgaagt aattaaagaa cgtattttga
     2341 agaaaaaaga gtttgccaat gattctttag ccttacaatt tgaaactgtt caaacagaat
     2401 tgaaaaataa attactcttt gttgatagtc ctgaattaaa gctcttccaa gatgctgaaa
     2461 attacgcaga agtttatcca tttattccct atcaattcaa tttattgggg caaattctaa
     2521 ccgagattcg taatcacagc tatcaaggat caaacttatc gtctggtgaa cgttcattac
     2581 tagcaatgtt taaagaagcc gctgaacgaa ataaggaaaa tgatacaacc agtttggttt
     2641 ctttagatca gttttattac tcgttagaac gttggattga tacagcaatt agccgagtga
     2701 ttacacaagc tacaaataat tctcatattc aaagaccaac tgatatagat aactttaacg
     2761 taaatgtctt acgaatttta ttcatgatta aatatgttga tcaagccatc aaaccaaatg
     2821 ttgaaaatat tacgagcttg atgataagaa atatcaacga agaccgcctg gaaactaaaa
     2881 aacgagtaga agaagcattg gaccttttaa ttaaggaaaa ctttgtgcgg aagaacttag
     2941 atagttatat tttccaaact gatgaagaac aggaaattac tcgtgagatt aaaaatcaaa
     3001 atattacaga tagtgatttg atcgtagaac tatctaaacg aatttttgac ggcgttctag
     3061 accgaaatag ttttgcctat gaaaaattag gcatgtcagg tggaaataaa tttaaaaacc
     3121 ggtatacgtt tacttatgca caagtgttag atcaacgtcc atacaagcaa gctggaaatg
     3181 ctgaaatgac agttaatttt atttcaccat actctgattt agcaggtcaa gaggcagaac
     3241 ttgcgcttaa gagttcccgt aacttattat tcgtagaatt gccatcagat aaagaattta
     3301 aagatgatat agcaatgtat gctagaatga atctttattt aacaacgact tattcgagtg
     3361 tagtaaagaa ctttgacgcg attcgttcgg aaaaatctgg tgaattacag gaactaagtg
     3421 caagtatcaa cgatcaatta aatgaactgt tgaaatctgc aaaatttttc tataacggtg
     3481 cagaaattca actgacaggc tccgacttta aagcaaaatt ccaatcagca cttcagaaaa
     3541 tggctgaaga agtataccat aaattaaacc aaattgatgc gcctaaagaa aaagaagata
     3601 tcttatattt agtgaaagaa agcaaaaatg aaattattga agatccatca aatgagcaag
     3661 cagttcagga aattttcaat tttatcaatg atcaaaagag aaatcaccta acgattacga
     3721 tgaaaacaat tcgtgatcgt tttgaaacaa aacaaccgta tggattcaca gaacttgatg
     3781 tggaatggtg catcgctaaa cttttcatgt ctggtaaaat tgatttactt tataacaatg
     3841 tacaaatttc acggatggat gatccaaata gtattgtaat gattattcaa aaacgacgag
     3901 aaagtgaaaa gataaaaatt gaaccaagcg cggtgatttc gccaaaagca aaaaaattgg
     3961 ttgacgaaat cggtagagaa ctgtttcaaa gtcgtaatat tgttgatgaa aataacaatg
     4021 aacatacaat ggaaaatttc aagaaatatt taaaaggtca attagacaat ttacgctctt
     4081 attttgattt acaaaacact tatacctacc ctagtgctta tgaatatgag caaataaaaa
     4141 aatatcttga agtaattgtt caaacgtcag atgttgaaga ctttttttat acaatcaaaa
     4201 aatatcgcga tgaattattc gactgggctg aaacatatga taaaattaaa aatttccaca
     4261 agcaaaatag tactcagaaa aaaatctggg agaatgcaca agataaaatt agattaatcg
     4321 aaaaaagtcg gcgtcaaact tctagccaag aaattgatca attgattcat gagatgaaaa
     4381 ccattttgaa aaaggctgat ccttattcag atatgatgaa actaaatgag ttgattcaag
     4441 aatttattga acaatataat caactgttag agttggaaag tcaggaaata ttaaataaat
     4501 taacggaaga aaaacaagca acatttaaat tattagaagg aaaagatttt aaggatcaat
     4561 ttgtaccaat agttgaccaa gaatttcaag aactgaaagc aaaaattgaa caagcgaata
     4621 ctatagatga tcttgttttc ttatcattaa acgtagcaga acagtatttg atctttaatc
     4681 aacgtttcga taatgaagaa aaccgcagac gtcaagaaga acaagcacgt aaacaaattg
     4741 aagcagcaaa cttgattcgt gaaactgaaa aagatgaaat agactctgca gcaaaagctg
     4801 aagaaatagc gatagtaaaa cctactattc aacagaaaac agtaccaatg agaacactta
     4861 acgttcatgt taaaactatt aagtcagcag cagatattga tgagttaaca gctgaaatta
     4921 atcaaaaact gaaggaacaa cttcaagaaa atacagaaat cacgattcaa ttctagtgaa
     4981 aagacaggca taaaaaggtg cctgtctttt caaaggttac atatagaagg gaaagaaaaa
     5041 tggataagaa agcgataaag aatttcgcgg tcaatgcccg tagaaaatta attgatgaag
     5101 taatgactaa agcaaaacgt ttaggaattg atgaatcaaa aattgaagat gtgaagaaaa
     5161 ttgatagcaa tttacaagaa atcgaaaaat caggaataag aattcaagga aaagaaatag
     5221 cgcaaagaaa caaattgatt gcagaactaa aacaacgtgc ttctgtcact tcttattcag
     5281 aagcttttca agagttaatc gaagaggttg cttatacgtg gtttaaccga ttgattgcca
     5341 ttcggtttat ggaggtaaat gattatttac ctgacacgta tcgtgtatta tctagtgaaa
     5401 taagtgggaa aattgagcca gatattatta cagatattta tgatgccgat atctttgaag
     5461 aattatctag taatgaacag caacaagtca gaacttggaa aacagataat tcagcagaag
     5521 caatggacaa gctgtatcaa ttgattttta taaaaatctg taatcaattg aatgattact
     5581 tacctgagct atttgaagaa gtagaagatt atacagaact tttgtttaca gcttcttata
     5641 ttaaaaaaga tggtgtaatc gctgatcttt taagtattcc agaggatgat ttctatgttc
     5701 aagctggtgg acaggtagag attattgggt ggttgtatca gtactataat agtgagccac
     5761 ataatcaagt ggtgaatatt tataaaggga ttgtcaaaaa acaagatatt ccggcagcaa
     5821 ctcaactatt taccacagat tgggtcgttc gttatatggt tgacaatagt ttaggaaagt
     5881 attggttaga tcattatccc aatagtgaat taagaaataa gttgaaatac ttaattccag
     5941 ataaatatgc ttttgaacaa gcaacatctg taagtaattt aaaaccagaa gaactaaaaa
     6001 ttatcgataa cgcaatgggg tctgggcata ttttagttta cgccttcgat gtattgatgg
     6061 aaatttatct tacgttagga tattctgagc gtgatgctgc acgtgcaatc gttgaataca
     6121 atttatatgg tcttgaaatc gataaaagag cgtatcaatt gtcttatttt gcaattatga
     6181 tgaaagggcg tcaatacgat cgtctttttt tgaaaaaaag tctatcgccg aatttaagag
     6241 agtttattga ttctcctaaa gtgacagacg agtatcaaga gcgtattagt gaattgaaca
     6301 atgataagta tagtgaacgc tggaatgaat acaaagagtt aatttttgat caatttgaaa
     6361 atgggaaaga acttggatca atcattaagt tagtcgataa aaataatcag gcaataacta
     6421 aagaatactt aggaaaagta agagagtata tatcaaaatt caacgaattt acgaatatgg
     6481 atattcttta tgggatgaag gaaatacaag aaaaatactt gcacattctt gacgtggctg
     6541 aaatgctagt tgataaatat gtagcggtag tcactaatcc gccttatctt aataaaatgg
     6601 ataagaaatt aaaaaaatat gtaaatgata actattctga tgtcaaaaaa gatctatttt
     6661 ctgtgtttat taaattgaat agccaaatgc tgattgataa tggatatgct tcctttatga
     6721 cgccatttgt ttggatgttc atttctagct atgaaaagtt acgctcattt ttaattaatg
     6781 aaagaaacat ttctagtttg attcaaatgg agtattcagc ttttgaagaa gcaaccgttc
     6841 caatttgtac gtttacgata aaaaattcaa atgaacaaga tggaagctat ataaaattat
     6901 cagattttaa aggtggaatg gaagttcaaa aaattaaaac gttagaagca attcatcagc
     6961 atggtgaatg cgattatttt tatagtacta atcaagacaa cttcaaaaaa atcccaggaa
     7021 tgccgattgc ttactgggca agcgataacg taatcactga ttttgctcaa ggaaaattaa
     7081 ctaacgatgt tgcagatgca aaacaagggt tggcaaccgg aaataacaat aaatttttgc
     7141 ggtattggtt tgaagtagaa tttaacaaaa taaagtttga tgctaaaaca ttagaggacg
     7201 ctaaaaagtc tagaaaaaaa tggtttccta caactaaagg tggtcgtttt agaaaatggt
     7261 atggaaacaa tgaatacgtg gttaactggg agaatgatgg agatgaaata aaaaattatg
     7321 ttaatgaaaa tgggaagtta aaatctagac cacaaaatct taattactat tttaaaaagg
     7381 gaattacttg gtcgacactt tcatctggac aagtatcttt tagattgcag aatcaggcaa
     7441 tttttgaaac taaaggatct gttctgttct caaaggatat aactgacttt gaagaaatat
     7501 ttggctatgt aaactctaaa ttagttaatc actttttaac ttttatttct cccaccttgg
     7561 attaccatga aggaccaatg ggaaaactac cttttcttaa agttacatct gagagaaaat
     7621 taaaaaaaat aagagaaaaa gtggatgaaa atatcgatat agctagaaaa gactataatg
     7681 ctcttgaaac gtcatacgag tttagaagaa acattttgtt aagttatatg tttaaaaaag
     7741 atctctcaga ggcatttaac tgtctaagta ggcagttggt taatcaattt aatactctaa
     7801 aatcgaatga agaggaactg aacgacatat tcattaatca ttattctctt gaaaaagaat
     7861 taacaaaaga ggttcaagat gaatatatct ctgcaaggtt acctgataag gcgagggaaa
     7921 ttaaggattt tatttcttat tcaataggtt gtatcttcgg tcgttactcc ctagacgaag
     7981 atggtctcat cttcgctggc ggcgaatggg atccttcccg ctatcaaaaa tttaaacctg
     8041 atactgataa tgttttactc atcaccgatg atgagtattt caaggatagc caaattgaca
     8101 ttgtcaatcg tttcattgat ttcgttgaga ttacttttgg taaagaaacc cttgaagaaa
     8161 atctccagtt tatcgctgac actttaggtg gcaaagggac aagtcgagaa attattcgtc
     8221 gttacttcgt acaagatttt tacaaagacc atttaaaaac atactccaat cgaccaattt
     8281 attggcaatt ggactcagga aaacaaaatg gctttaaagc gttaatgtac ttgcatcgct
     8341 atacaccaga tcaattagga aaagtacgta ctgaatacct acacgaacta caaaaagcgt
     8401 atgacaatcg aatcgaattg cgtcagcaac aaatttcaac aagtgataac ccaaaagaaa
     8461 gaaatgcaat gacaaaagaa attgtaaaga ttcagaagca attaaaagaa gttcgtgagt
     8521 atgacgagaa gttgggtcac ttagcattaa aacgaattga attagactta gatgatggtg
     8581 taattgtcaa ttatgataag ttgcagcgtg atcctaaaac cggcgagaaa ttccaaattt
     8641 ttgcaaaagg ggtcaaagaa ccaaaaaaat agttatctaa gaaagttgtt ttctaacaac
     8701 tttctttttt attcttaaat tgcgtgcgaa attatccaaa ttatgtcaat ataaggggta
     8761 tagaaagaga acgattaggt gagattgatg acagatataa atacgcaaca aattgaagaa
     8821 aaattaaatc aacgttttcc taattatgga tgtaggaaaa tcattttttg gtttgattca
     8881 aatcaggatt ttcttgaaga aattgactca ttggtcattc aaaatgcaaa aatctatcga
     8941 ttagaaaagg acgcacaatt taaaactaag cgtttattgg aatatgaaga tcccgaaact
     9001 aactatttaa tctatgcgcc atttcctcgt tgggatgata aagatgaaga taatcatttg
     9061 ttgagtgtat tgaattattc agaggaattt agtgcagacc gcttagcgat tatcatgaat
     9121 gaattggcta ttccacttag atttcatcag acagttaaac agtatcgtaa tttctttaat
     9181 gcaaaggatc gcatgagaag ttttggtgaa tttagtacgc atatgacaat taataaatca
     9241 gaaacgattg aattaatgat aatggctgtt ttgttgaaat cgaaaagtat tcgattaaat
     9301 gatttactgc gtgtagttct aaaaagcttt acagaaagta gggagaatcc tttaagggaa
     9361 cttgataagt atcagttaac agctaaattt tgggaattta ttcaaatgaa atttggcttt
     9421 acggatagcc agccaacatt ggaaaagctg acgatgtgtc tttatttgaa ctatttttat
     9481 tatcaaattg atcaagagat gccgaagaag ttcaaaggat ttgaaatact acatttgaaa
     9541 gcaaacatta ttactttcat ggatcagttt atgaatgata cacgttacat ggaaagcttt
     9601 gaacagctat ctaaaaaagt atatcaggct attgatggtg atggattatt agacagtata
     9661 gaaattgatg atctaattga agcagacgta ttttctgaga ttcatgcaaa aatattaatg
     9721 ttttatgcag aacgattaaa ggcttctgac actacaacga aaattgcagg acaaacaatt
     9781 tttgatagtt taaagcagcg cgaaaaaatg catttcgcat cggattatat ttatgagtat
     9841 caattattga aacatgctta ttatcttgtg gataaaaaca gattgtgctt gccgatgaac
     9901 atggataatt tgctaactca atatgagaag gaaatgtatt gggttgatac gcattatcgg
     9961 aaatttattt ggaatagtga tcatatagaa gaaaagcaaa aatatggcca attgtatcga
    10021 ttagttgaag caaattattc gaagtttctt gatcaatcag gacaaatttg gaatcaaact
    10081 tttgaattta agaatcgtcc atccattcga aaattttata atcaagctgt aatgaaacgc
    10141 tctgttaaaa ctgtagttat tatttcagat gcttttcgat atgagttagg gatgaattta
    10201 gaacgtaagt tagttagaga aaagaagtat tcaacaaaca tggctagctt tctttctgta
    10261 ttaccttcag ttactgaatt cggtaaagcc gccttattac caaacaatca actaacttac
    10321 aatgaaggaa cagaagtact gattgatggg aaaaaaacga atggtttatc caatcgaaat
    10381 gcgattctcc agcaacgaga tgcaaatgct gtagctttaa attatgatga tgtggttaag
    10441 atgaataagg ctgaggtacg agaagtattt aatggcaaat cagtcattta tatttatcac
    10501 gatcaagtgg atagaacagg tgatcactcg aatgaaaatc gagtttttga ggcctgtgat
    10561 ttagcgataa gcgaaatttc ccgacttatt aatcgcttgc ataatgatgg cagtatctat
    10621 cgttttatag ttacggctga tcatggattt atttataaaa gaagccacgt tgaagaagta
    10681 gataagattg aaaatccatc tagaaatgaa aaagatcgtg tagaacgtcg ttttattctt
    10741 agtgaagaag aatacgaaat aattggtacg aaaaattact ctttgggaga ttctttagga
    10801 aatgatgaca ctagaaacat ttgtgtcccg attacttcaa gtatttttaa gaaagctggc
    10861 gggggacaaa gctacgttca tggagggtca tctattcaag aaatgctagt ccctgttcta
    10921 gaaattacgg tagccaaagg tagcaatgca aaggaaccag tagcaattga attaatgact
    10981 agcaaccgca gaattacagg attatcaacc acgttagaat tctatcaaaa aaatgcaatt
    11041 agtgatgttt atgaccccgc atctttctgg ctttattttg aagatgaata tggtgaacgt
    11101 atctcaaatg aagaaatcta tgttgcggat tcaaaaacag aagatgcaaa tcagcggttt
    11161 actaaatttg tttttgaatt tgtaaatcgt cgatatacag ttgaagatac ttattattta
    11221 gtgataaaaa atcaaaaaaa tgatttgacc gagcgaataa aattcattat agataatcct
    11281 tttgcagaat catttgattt tgatatataa aaacgaggta tccacaattt tttgtggata
    11341 ctttttcttt tgtattatca agcctactgg ttaagtatga tatactttaa ttgctattat
    11401 aatgactgaa cacatggagg aattctagtg gaacaacagg aaattttaaa gttatctgaa
    11461 ttctttcccg gaaagattgt cagaaaagat ttaacaaaga aaattaaaga aggtgcgaat
    11521 gtacctgttt atgtattgga atatttactt ggtatgtatg cttcgtcatt ggatgaaaca
    11581 ttggttgaaa aaggtgtgga aaatgttaaa aatattcttg ctcgaaacta tgtgcgtcct
    11641 gatgaagcag aaaaagtaaa agcaaaaatt cgtgaattgg gtggttatac agttattgat
    11701 aaagtaactg tacatttaaa tgaaaagaaa gattgttatg aagcagaatt ctctaacctg
    11761 ggattaaaaa atgttcaaat tagtgaagag tatccgaaaa aatttgatcg attattaggt
    11821 ggggggatct ggtgtattgt ttcgatggaa tattggtatg atgagaatga tcgttggaat
    11881 agccctttta ttgcaaaaaa ggttgatcca atccaaatgc ctggtttaga cattgaggct
    11941 tacaagatgg ctcgcaagga gtttactaca aatgaatgga ttgacttact tctacggtcc
    12001 attggtatgg aacctacaca atttaataaa agggtaaaat ggcttcattt agcaagactt
    12061 attccattaa ttgaaaataa ttttaatctc tgtgaactag gaccacgtgg tacaggtaaa
    12121 tcgcatgtat ataaagaaat ttcaccaaac tcaatccttg tttcaggtgg acaaacaaca
    12181 gtagcaaacc ttttctataa tatggctagt cgtacaatgg gacttgtagg tatgtgggac
    12241 tgtgttgctt ttgacgaagt tgctgggatt aaatttaagg ataatgatgg cgttcaaatc
    12301 atgaaagatt acatggcttc aggatctttt gctagaggca aagaagaaaa gaatgcttca
    12361 gcatcaatgg cttttgtagg aaatattaat caaagcgttg atgtaatatt gaaaacttcg
    12421 aatttatttg agccatttcc ggaggttatg gggacggatt cagccttttt ggatcggatg
    12481 cattgttatt taccaggttg ggaaattcct aaatatcgac cagatttttt tacaaatgac
    12541 tatggcttca ttacagatta tttttctgaa gtcatgcgtg agcttcgaaa agttagtttt
    12601 ggcgatagtt ttgataaata tttcaaactt ggttctcaat taaatcagcg ggatacgatt
    12661 gcagttcgtc ggattgtatc tgggatgatg aaactgttgt atcctgatgg tagatttgaa
    12721 aaaagtgatg tcgaagaaat actaagattt gctcttgaaa tgcgtcgtcg tgtaaaagaa
    12781 caattaaaga aaattggtgg aatggaattt tatgatgtca atttttcata cattgattta
    12841 gaagaagagc gagaagagta tgtttctgtt cctgaacaag gtggaggaac tttgattcca
    12901 gaagggttag ggaagccagg gcatttgtac acggtatctt atggcaaaac tgggcaatta
    12961 ggtacctata aattggaaac gcaaatgact tcgggaaatg gaaagtttga acgtactggg
    13021 atcaattcag atgctggagc aagagaagcg atggattcgg cataccgata tttaagagct
    13081 aattatggaa gtgttagtca gtctattagt ttgacgacaa aagattttgt cgttcatgtt
    13141 caagatatga atggcattgg tatgacaaaa acattatcgt tatcaactat aatagcttta
    13201 tgctcagttt ccttaaataa acctgtatta ccaagtttag ttattttagg ggatattaca
    13261 attggtggtt ctatgattaa ggtagataat ttagctaact gtttacaagt agcaaaagat
    13321 gccggagcga aaaaagtact tgtacctatg ataagcgctg ctgatattgg aactgtaccc
    13381 ccagaattaa tgggtacctt taatttgatt ttttatcaaa cgccggaaga tgctgtcttt
    13441 aaagcccttg gggtggaata gacaactttt ccatttgctg gtttgactac gtaccaatct
    13501 atattactta ctagattagt cgttaatcta ttcggatcga atgggcaaaa tatttttgtg
    13561 tatgtccgtc cttgatcatg attccaaaaa gaatcattgg gggctgtggc gtaaatatta
    13621 tactataagc
//
LOCUS       DAZKCE010000076        13435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000076 DAZKCE010000000
VERSION     DAZKCE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.077"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..1333)
                     /locus_tag="ACG8KQ_002014"
     CDS             complement(71..1333)
                     /locus_tag="ACG8KQ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104937.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1326..2225)
                     /locus_tag="ACG8KQ_002015"
     CDS             complement(1326..2225)
                     /locus_tag="ACG8KQ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104938.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2370..4148)
                     /gene="uvrC"
                     /locus_tag="ACG8KQ_002016"
     CDS             complement(2370..4148)
                     /gene="uvrC"
                     /locus_tag="ACG8KQ_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9104939.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4330..4644)
                     /gene="trxA"
                     /locus_tag="ACG8KQ_002017"
     CDS             complement(4330..4644)
                     /gene="trxA"
                     /locus_tag="ACG8KQ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9104940.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4731..7091)
                     /locus_tag="ACG8KQ_002018"
     CDS             complement(4731..7091)
                     /locus_tag="ACG8KQ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9104941.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7206..7754)
                     /locus_tag="ACG8KQ_002019"
     CDS             complement(7206..7754)
                     /locus_tag="ACG8KQ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFP9104942.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7812..8249)
                     /gene="zapA"
                     /locus_tag="ACG8KQ_002020"
     CDS             complement(7812..8249)
                     /gene="zapA"
                     /locus_tag="ACG8KQ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9104943.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8451..9368
                     /gene="rnhC"
                     /locus_tag="ACG8KQ_002021"
     CDS             8451..9368
                     /gene="rnhC"
                     /locus_tag="ACG8KQ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9104944.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9746..10657)
                     /locus_tag="ACG8KQ_002022"
     CDS             complement(9746..10657)
                     /locus_tag="ACG8KQ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9104945.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11058..11636)
                     /locus_tag="ACG8KQ_002023"
     CDS             complement(11058..11636)
                     /locus_tag="ACG8KQ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9104946.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11656..12324)
                     /locus_tag="ACG8KQ_002024"
     CDS             complement(11656..12324)
                     /locus_tag="ACG8KQ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104947.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12339..13409)
                     /locus_tag="ACG8KQ_002025"
     CDS             complement(12339..13409)
                     /locus_tag="ACG8KQ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104948.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3821 a         2884 c         2218 g         4512 t
ORIGIN      
        1 aaaataggtc tatacaaaaa gaagctggga acaatagatg tccagcttcc ttctgtattt
       61 taggattgtt ttatgctttt tttcgttcat tcctcataat tgaaatcgat tgtttcaatg
      121 aactccataa tccgccttcg ctataaacga gtacattcga tttgtacatg tttgcagaca
      181 agatcatcaa taagaatgtt acggctacaa gtaccaacaa ggaaatcaat gctccaccca
      241 taccgactgt ctcatttgct aagcgaatcg gcataatata tgaagataag aatggaatat
      301 acgaagtgat ccgaataacg atattgtttg gatctgaagc tcctagtatc aatccaagca
      361 tgtatccgcc tagagataga taagtcactg gcaaaattgc ttttgctgta tcttctgctt
      421 tgttgaccaa agagccacat aaagctgcta aaacagaaaa aatcaaaatg ccaaagatca
      481 tgaatagaag agaataccac aagaatggtc caatcaagtt atccaatgag ataccgtcta
      541 atatattttt gaccatttcc atatctttga attgactgta tcctatacca aaaataatac
      601 cgtataatgc catttgcgtt aatgcaacaa gtaaaacacc tgttaatttt ccgtaaaaat
      661 gcttttgtgc agtcgtactg gacaggatca cttccatgat ccgcgtgcct ttttcagaag
      721 cgatctcttg tgcaatgatc tgcgcatacg tcaaaataat aatgaacaag acaatcgttg
      781 ctacataact tacgacgtat tggactgtcg agttatcttc tccgactgtc atttttcctt
      841 ttgaatcaaa attgactttt tgtcgagtca aacttgcttg ttggctcaag ctagctactt
      901 cttctggtga aatgcctaat tgactcgctc gaagcatcga ttgcaagcca gtcagttgtt
      961 gttgtatcag tagttgtgta ctttgtccta atgaattttc gctataaagc atcccattta
     1021 tcttatcatt ctcagttgag actactaaat acgcatcgat tttttcttcc gaaagctctt
     1081 tttgtgcttt ttcttcagaa tcaactgctt tgaactcaaa atcatctgtt ttgacttggc
     1141 tcatttgctg actgatttgc tcattatcag ccaatacgcc gattttattg acatcactat
     1201 tttgttgagc aaagtttcct gctacataaa tgatacccag catgataaaa gggactaata
     1261 tcatgatcaa gaaagaaatc gacttaacat tttttaaata gacatctttg gtaataatcc
     1321 aaaacttacg catcgatttc acctgctttc aatttgaaaa tctcttccaa agttggcggt
     1381 tgctggttaa acataggaat gtagccgaac tgagtcgcac gagtaaagat ttctttccca
     1441 gcttcaggat cttctaaggt aatctctaac gtattgtctt catgaaggat cacttttttc
     1501 acgccttcga tttcttctac ttcctgttgg cttaaaccag attccaagaa taacttcgta
     1561 cggccaaatg attcacgaat ctcatgtact tttccattca aaaccatttt tccgtttcgc
     1621 aacatgatca aatgatcgca gattttttct acattatcca tattgtggct agaaaaaatg
     1681 acacatgaac catgtttttt caattccaat attccatctt ttagcaattc agcatttacc
     1741 ggatctaacc cgctgaatgg ctcatccaaa atgatcaatt taggttcatg gattaacgtg
     1801 gcgatcagct ggactttttg ctgatttcct ttagacaaag attttacttt atccgtcttt
     1861 ttccctttta cctgaaattt ttccatccaa aagtcgattt ttggttcaat ctcttttctt
     1921 gatttccctc taagttctgc aaaataaagc agttggtctt gaatcgttac tttcggataa
     1981 agtccccgtt cctccggaag ataaccgatg acatcataat ctttctcccc aagatgatgg
     2041 ccattccata acacttctcc ttggtcttgt gtcaaaaaat ccaagatcaa acgaaaagtc
     2101 gttgtcttcc cagccccgtt ttggccaatt agccccatga tttttccatc ggggatttgg
     2161 aaagagacat gatcgacagc tgtgtagttc ccaaaagttt taacaagatc cttgacttct
     2221 aacatttctt cactccttct tcaagaaatc acatatatcc ttagacacca gacgtaagcg
     2281 atttattatt taccaacaaa acgtaaacgc tctttttgtt gtacataaaa tgataggaca
     2341 ctttactttc tttgtaaagt gccctttcct cattcctgat gtaatttatc aaatacgttt
     2401 tgtgcgacat ttttcggcag tccgatttct tgtaattctt ctgtcgtggc tgctgtgata
     2461 tttttcaacg atttgaactc tttaagcagc attttttttc gttttggtcc taacccttca
     2521 atttcatcta atcgggaagc aaaactgttt ttactgcgta gctgccgatg gaaagtgatt
     2581 gcgaatcgat ggacttcatc ttggattcgc tggagtaaga aaaactctgg tgaatttcgc
     2641 tcaagcggta ctacagccaa atctgtaccg aatagcaact catttgtttt atgtttatcg
     2701 ttttttacca tgccagcaat aggaatatcg attcctaatt gattttctaa gacatctttt
     2761 gccacatcta cttgtccttt tcctccatcg atcaagatca aatcaggtaa aggcaaccct
     2821 tcgcgaatca ctcgcgaata tctgcgatag atgacttctc tcattgatgc ataatcatcc
     2881 ggtccgttga ctgtttttat tttatatttc cgatattctt ttcttgccgg ttttccatca
     2941 atgaatacga ccatcgcagc cacaggattc gtccccatga tattagagtt atcaaaagac
     3001 tcgatacgta ttggggtcgg gatattcatc gcgtttccta aacgttcaac tgctccgatc
     3061 gtccgttcct gttttctggc aatcagatca aaccgttcat tcaaagcaac tgaggcattc
     3121 ttactcgcca attcaactaa ttttttcttt tctcctcgtt ttggttgcag cactttcgta
     3181 gctaacaaag cttctacact gggcttgtcg atcgtatcag gaatcaagac ttctttagga
     3241 atgaaatgct cattttcctg ataaaactga ccgaggtaag tcaagaaatc ttcttctgct
     3301 tcgttataga aaggaaacat tgaaacatct cgttcaatta gctttccttg tcttacgaaa
     3361 aacacttgga cacacatcca tcctttatca atcgcataac caaatacatc tcgatccagt
     3421 aaatcagtat ttgtcatttt ttggcaagtc atgattgtct caatggcacg gatctggtct
     3481 cggtattctg cagctttttc aaactccatg ttttcagcag ctttttccat tttctgatgg
     3541 atctctgact cgattttttc gtgtcctccg tttaaaaagt ttttcacttc atcaacgatt
     3601 ttcgtataga ctttcggatc tacgtcgaaa taataaggac acaggcattg tcccaaatga
     3661 tagtaaagac aaggctcctt cgtctgacta ggtttgcatt ttctcagagg aaataatcga
     3721 tcgaggatct tctttgtctc attcgccgca ccgacatcgg gatatggtcc aaaatacagt
     3781 gctttgtctt ttagtacctt acgagtaatc accaaacggg gatatttttc attcgttatc
     3841 ttaaggaaag gataggtttt atcatccttt aacataatgt tgtatttagg atcatttttc
     3901 tttatcagat tgatctctaa gagcaatgct tcgatattcg attctgtaac gatgtattca
     3961 aagtcttcga tttcactaac aagccgttcc gttttcgtat tatggctacc agtaaaatat
     4021 gagcggacac gattttttaa tattttggct ttgccgacat aaatgatcgt tccattttta
     4081 tctttcatca agtaacagcc gggctgatca ggaagtaaag ctaatttgtt tttgattcgt
     4141 tcgttcatct ggtactcccc ttcatattca attaaagtta ttatagcatg tctgtcaaaa
     4201 aaacaatcca gcctgacaac gtaattatac tcaaaaaata ttaatttgaa taaccttaac
     4261 tcaaccgcaa aaaaaccgag gacggaactg gattcagtcc caccctcagc ttatttttgc
     4321 tgtaagtttt tataaatatt gatcgatcat ttgacgtagt tgttcttttg aatgaacacc
     4381 aacagctttt tctactactt gaccatcttt tttcaacatt aatgttggaa tgctcatgat
     4441 accgaattgt tgaggagttt ctggattttc atccacatcc attttagcaa ttctgaattc
     4501 ttcttcatca tattcttgtt ccaactgatc caaaatcggt gcttgcatac gacatggacc
     4561 acaccaagtt gcccaaaaat caatcaatac aagtccttta tctgtttctt cattaaatgt
     4621 cttatctgta attacttgtg acatatcaaa gacctccatt tttaaaatct atgactagta
     4681 tagcatttct atacttggat agcgaaccat ctgcttacct ttagtaagct ttacttaaat
     4741 ttaacaattg tcgcaccatt tccgccttgg tttgccggtg caaactcaaa gcttttgacg
     4801 ctccgatggt ttttaagata atcagtaatt cccttgcgaa gtgcgcctgt tccttttccg
     4861 tggacaatag tcacttgagg ataaccagct agaatggcag aatccaaata ttgatctacc
     4921 tcacttagcg cttcttcgta tcgttttcct cgaaggtcca attgattcgg cacgtgacta
     4981 ctagattcag atcgtacagc atggataact cgttgtgtcg gctctttttg aggagcaaca
     5041 ggcgtcagct cgtcttcgga cacattcatt tttaatatac cgatttctac ttgccactga
     5101 ttcccgtttt tacgtaaaag cgtccctctt tggccgtaag tcgtaaccaa cacttcgtct
     5161 cccggtttca acgttttttg ttctttcgcc ttcttcaata ctttattttt cttcaaagtt
     5221 tcttcttgat gtagatttgc taattgggtc tttgcatcga tcaactgatg ctctttcaca
     5281 tttccttgcc caatctgctg ttgcatttta cgaatatcag caatgatttt ttctgctttt
     5341 tcttccgcct ctgacacgac ttcatttgct tttttctttg ctttttccat ttctttttca
     5401 cgttcttcaa agaaatagct gtaagcttct ttcaaatcat cgtgaagttc ttgtgcctca
     5461 gaaacaaaat gacgcatttc cagatattcg gtctctgcca ttttccggcg attctctaaa
     5521 tctgtgatca tttcgtttaa atcttggctc tcatcattca tcaactgctt cgcttcatca
     5581 ataacttctg tatcaagacc taatcggcta gagatttcaa acgcattgct tcgtccagga
     5641 acgccaatca gcaaacgata cgttggactt aatgtatcca catcaaactc catgctggca
     5701 ttaatcgtat ttgcccgatt atatccataa actttcaatt caggataatg cgttgttgcc
     5761 atcacataag cagacttttt accaagatca tctaagattg cgattgctag agcagcacct
     5821 tcttgcggat ctgttcctgc accgagctcg tcaaataata ccagactttt ttcattgact
     5881 tttgataaaa tatctacagt gttcgtcata tgagaagaaa aggtcgatag actttgctca
     5941 atcgactgtt cgtctcctat atccgcaaat acttcctcaa aaataccgat ttgactttct
     6001 tcacctgcag gaatcggtaa tcctgcttgc cccatcagtt gtaacagacc taaagtcttc
     6061 aacgtgatcg ttttcccacc tgtgttaggt cctgtaatga cgatggcttg atagtctttt
     6121 ccgattgtga tgtcattaga aactactttt tcctgatcga tcagtggatg tcttgcttgt
     6181 ttcaagacga catgattgtc ttcactgatt cccggcacga tggcttttac ttctttacca
     6241 aatcgtgctt ttgcattcat cagatccatt ttaccaatca cataagtatt gtggatgatt
     6301 tcttgacgat ggggaacaag ttcagcagaa agttcactta aaatacgttg aatctcattt
     6361 cgttcagcga tttggtattg acgcaaacga ttattcaaat ccaccacttg ttttggttca
     6421 ataaataaag tctgacctga agcactttga tcgtggacta caccgccaaa tacaccgcgg
     6481 tattcttgtt tgactgggat aacgtagcgg tcatttcgca tggtgatgat cgcatcactt
     6541 aaatactttg catttttccc acggacgatc ccatctaact gttcacgaac agcttgttca
     6601 cttcgccgaa tgttctgtct tatgctcttt aactcaggag aagcgtcatc agtcacgcgt
     6661 ccgtcttcat cgattgcttc ctttaaacgg cggctcaaaa cagggatcgt gactaactga
     6721 tctgcccaca tatacaaacg tgcaaattcg atttcactgt cagaaagatc atcaatgaaa
     6781 cgattcaatt cgcttgtcgt agacaatact ctggaaactt gtgcaagctc cactccattc
     6841 aagtctgctc cgatctcaat ccgtttcata tggggacgga tattttcaat tttaggtata
     6901 gggatcccgc ctcgcagacg ttgcactttc aatccatcct cagtttcttg aagccagttt
     6961 gtgatcgttt gtttctcact cactggtacc aattctgcca gttcttcttt tccttgtgca
     7021 gtcactacaa attgtctgac catctgtttg actttttcaa attccaatgt ttcaaggatt
     7081 cggttattca ttttatcacc ttatctttca ctatgaaaaa agagctgtgc cggacatacg
     7141 ctacgcagcc ttcttaaata aactactctg tacaaaaaac gaaatatttt ccagcactat
     7201 tttccttaat tcaaaatatt cgttacccaa agctcataca tcttgttggt aagaaatggc
     7261 gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg gaccacatct
     7321 aatggaacaa aggcaagtaa ctgcaaaatc aaaaatatag taacgtaagc catgattagt
     7381 gacaggatgc ctcctgcaat ccagtctacc tgtctcaaga tcggcacata cgtcaatcca
     7441 tggacaaaaa cccccacaaa tcgggtaagc agccagccaa ttagcaaaat aatcagaaaa
     7501 gcagttcctg cataaaaagc ttcatctagg tgaaaggcaa atacctgatc aaaaaatgct
     7561 aactttgatg ttggggtgac agctgggtat ggaatgtata attccaaatg acttgctaat
     7621 ggtttgtaaa aatgctgggc agcaataaat gacaatacat atccaatcgc gaatatgatt
     7681 tgcaaagcaa acccgcgacg tgctccagaa aaaaacgcaa ttagcaataa aaataaaatc
     7741 agcaaactca gcatgctacg ttcctttctc ttctaacttt tttaaatttt tcttattttg
     7801 ataaaaggat cttaattaga agatttttgt ttaatttgtc cttttcgttt ctcgtttaaa
     7861 atctgttgtg cttccacatg atttttaatg gtatgatctg cttgaccatt ttctttcaat
     7921 acttcccgag cttcgttctc gattgcttca atgcgtttca cacgattttc aagctctgta
     7981 aatttgatca tttttttctt taattctgct gtttcttctt ccagctccaa tattctttct
     8041 tgtttcttca attgatccga aagcgcattg acagccatca agattgctgc ttgttcatta
     8101 tccatttgtg gtgataattg tttcaattct gctaactgtt cattgatcag cttcgttacg
     8161 atatccatat gatgcttggt ttcccgtccg ataatagtat atgtttggtt ggcaatcacc
     8221 gctttgtagc gtgttttttc gtgcgccata cgaatcattt cctcctattt gatgaaagtt
     8281 ttatttagtg taccatgaag ttggttcttt caccgttctc cacaaatatt tttcacatac
     8341 ttaactatta tatgataaac tttaaaaaaa tgctatgtct cgcaatctct tagaaaacga
     8401 aaagtcgtga gttgcggtat agttttatgt ataaaaacga ggagaatttt atgagcaacg
     8461 tcattatcaa agcaacaaac gaccaactta gtcaaatgaa agcatactat cgctcttatt
     8521 tgttaaataa acagattcct tatactagct ttgctgcaaa aaaagacggg acgaccatta
     8581 ccgcttatac atccggaaaa gtgatgtttc aaggtacgaa tgcagcaata gaagctgata
     8641 aatgggggga ggcacaggat aaacaagcca agccagcaaa aacagcctct tctttgccaa
     8701 aaaatatcag tcaattatcc gttttaggta gtgatgaggt cggaaatggc agctatttcg
     8761 gtccgctaac agtatgtgct gcttatgtga aacgtgatca gcttgcagaa ttacgtaaac
     8821 ttggtgttag agattcaaaa gagttaaaag atagccaaat catccagctt gctgacatct
     8881 taaaaaaaac catcccctat aaactgcttg tcttatctcc agaaaaatac aatgagatcc
     8941 aaccaaaata caatgccgtt cggatgaaag tcgctttgca caatcaagcg atccatttgc
     9001 tgctaaaaaa aatcgagcca gaaaaaccag aagcgatttt gatcgatcag tttacgccgg
     9061 aagcaaattt taaaaaatat gcgcgtttag aaaaaaatca attacaagaa aaactgtact
     9121 tcgttactaa aggagaacaa tatcatttag ctgttgctgc tgcttctatc atcagtcgtg
     9181 ccagcttttt ggaagagtta gacaaagcat cagctgaagc tggaatcacc cttccgtcag
     9241 gagctggaac gaaatctgac caagtagcgg caaaactatt ggagaaaggc ggcatgccta
     9301 tgcttgaaaa atatgccaaa cttcattttg ccaatacaga aaaagctttg aagctactaa
     9361 aaaaataatg gaactgtgac aaaagtcatg tcacagtccc ttgttccgaa taaacggtgt
     9421 tcaatcatct gatcctcggt attagctcaa caaacggcaa aatatgagga actatttttc
     9481 cgtttgtttc tctaatactc agaacaaaac gttgatttca caaccttttt cttaaacggt
     9541 gttctcatgc caactcctcg gtcacaagtc acaatagtca tcaaacatga gaagctgttt
     9601 tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgacctc ttccttaaac
     9661 ggtgtttaaa agcttgcatc tgcgataata agcccggcca aacgaaaaat atgaggagct
     9721 atttttgttt ggctgggctt atttattact gaaaataagt ggtcaatgct tgtgtcaaac
     9781 ttgcagcaat ctgctgctga taactgtctg ttactaattc ctttaaatca ctatcattat
     9841 tcatataacc catctcgatt aaaatagaag gttgaagatt ttctcgaaga acttgatagt
     9901 tcccagtgga gattcctctt gaatccagag gcagatcagt gaaggagtca gcaattgtct
     9961 gtgctaatgt ttcctcttgt ccatagtaat aatatgtcgt gattccagtt gcttcattcg
    10021 catattccgt agagtctgcg tgtaaactga taaagacatc tgcctttttt tcttcactgt
    10081 agtcgcatat atctccaagc tgtactaatt catctgtagt atgggtcaaa taaaccgttg
    10141 ctccagcatc ctctagcgcc ttttttactt ttatggctgt acttaatgtt atatcttttt
    10201 cttctgtttg ttcatcattg cttaatgccc cagtatcatt tccgccatgc ccaggatcta
    10261 atacaatcac tgcatcagat agctcttccg gttttgtttg tatattcttt gtgactttgg
    10321 ttccatcttc gagggctaaa tatccagtat agccatcatt tgtcttgatc tgataagcat
    10381 tttttgtttc agaaagaaac tccgccagct ctccgccgtt tatagttgca agtggggtag
    10441 ttagcgagtc atctgtatat agtgtacggt cattttttcc gattttgatt aatgtctctt
    10501 gttctgtatc atcaaatgat tcctttgtct ttgtaaccgt actgttcgta ctgctgaccg
    10561 tttgtccaac tatctttttt tgaccaaaaa catctatttg aaaacagata ataatgataa
    10621 tgattgcaaa tacaaaagct ccaattattt ttttcattct tgactctcac ctcgtagatc
    10681 cagactaagg gccaaaactt aaaatcagct taagtaagca aaaaaggggc tgtaaaagat
    10741 ataaccgtag aaaataacat tactgctaaa atgatttttt tcatttttgg catatcctga
    10801 aatctccttt tgactattcg tggtgattca tctacccaat aaatagtttc cttcattcta
    10861 caaacaatta atttcttcgt tattaaatta aaacacttcc tggaacaccg acataataaa
    10921 taaaaatata ccctgataat catgagagaa taacaaattt gtatttatga aaaactacct
    10981 atataaagaa gcaagatata acgatcttcg tcatatcttg cttcttcacc taactatttt
    11041 ctccctatat aacagcttta cgcttctcct aatacaccat tcaaatataa atcgatcaat
    11101 tgcgccacat ttagcggttc ttccacaggt cttttcgacc gagtgatgta aggtgctaac
    11161 actaagaaaa aatgccttgc tgcgatttct tttgaaatcc catttcttag cacagattgg
    11221 tttgctgaaa agatttcttc aaaaggttca cgataatttt tcttccatag aaagaaaaga
    11281 tgatctcttg tttcctttga aagattgctt tcaagatcgt gaagcatcag aaacaaatcg
    11341 accagatgtg tctcaatcaa aaactgcgcc atttttgtca aggttacttg aaatcccgtt
    11401 tcgcttgttt gaacgatttc tctcaaagct tttccaacat cttttaagtg gttttctaaa
    11461 actgccgtat atagtttttc tttatcaccg aaataatgat agagattggg ctgggtgatc
    11521 ccaacttttt gtgcgatttc tcttgttgaa gtgccgagat atcccttgga cataaacaag
    11581 tcagtcgctt ctttcaaaat tttttgctgt gtatctttgt tgtatcgatg gaccattttt
    11641 ccacctgctt tttggttatt ctttttcttt caatacacca tcttccatca taaatattct
    11701 atcgcatttg tcaatcagcc gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc
    11761 ttttgattct tttgccaaga tatcgactac ttctcgtgcc ttttctgaat ccaaactagc
    11821 agttggttca tctgctagaa taatggacgg atcattgtac aatgcacgag cgattgcgac
    11881 cctttgccgt tctccaccag atagttcttc tggatacttg ttgatcaact tatccacgcc
    11941 aagctgctca aataattcgt ttgcttcttt ttgacgatgt tcatgtttaa tcttatctac
    12001 taataataac tgctttttta ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa
    12061 gccaatttct ttaaaacgta gtttaacgcg ttcgctttct ttttgattac tgaattcttg
    12121 atcattgata aatacagagc cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa
    12181 agtactttta cctgaaccag aaggaccgat gattgcaaca aattcccctt tatcgatact
    12241 catggttgtt tctttcaatg ctttaactac ttggtcgcca tcttggaaac ttttttctac
    12301 ttctttaaat tctattgctt tcatgttctt cctccttctt atccaattgc cttcaacgga
    12361 tcgattttta cgattgtccg aacagaaaat agtgcgccta acacagctac tactaacatc
    12421 aacaagctaa tgacgccaaa aaacagccaa ttgctttgga atggaaccgc atcaggtaga
    12481 acaagggatg ttcccactgt tcctaataga ccaagaagaa taccaacaaa tgataaaatg
    12541 aatgtttgtg ccactacaga acgcgcaata aatcctcctg tgatcccttg tgctttcatt
    12601 acaccaaaga tattaatctt ttgcatcgtc agtacataaa tgaaaatccc gatcacaata
    12661 gccgcaataa cgatcaaaaa tccgatcata aatccaaaag tcaatacttg cgcattatat
    12721 ccaggtaatt cattgatgaa tttagaaatt ttgatctgtt ctaaatcttc aggtaaatta
    12781 ctgattttac ctctgacaac tattgcgtta atccgtgcat tttcacttgt atcttctttt
    12841 tcgaagcgga tttcttggta agcattgatg gttgtataga gaactggtga gacattgaat
    12901 ttggcatgat ccgtaaagcc agttagttta aacgtcttgt cactaccaga taacttaact
    12961 gtatctccta tggctagacc atattcttct ttcaaactaa tatcgcctac tgcctcatca
    13021 tcgttatcaa aagcttttcc ttcaacaaga ttgggcatga tgaattgatt tttatcgata
    13081 ccgaagaagc taacattgat ttttccttct tctgtactgt ctttactagt cacgactcca
    13141 ggtgtttggg caagatacgc cactttccca ccttcgactt cttctgctat cttcttagta
    13201 atcatggaca tccctagatt tgaatttgca tctttcgaaa gcacaatcga atctgcctcc
    13261 cacttgtcga ctgctgttcg attatcttga gctaagccat aagctaaccc agtcaaaaaa
    13321 aataccaggt aggcgatcaa aaacatcacc cctgcaacta acgcataacg taactttgaa
    13381 tgcataattt catttaatgc taaaaacatt taactcctcc tcctgaaata atcat
//
LOCUS       DAZKCE010000077        13232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000077 DAZKCE010000000
VERSION     DAZKCE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.078"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..1036
                     /locus_tag="ACG8KQ_002026"
     CDS             167..1036
                     /locus_tag="ACG8KQ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9104949.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1245..2270
                     /locus_tag="ACG8KQ_002027"
     CDS             1245..2270
                     /locus_tag="ACG8KQ_002027"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9104950.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2309..3259
                     /gene="manA"
                     /locus_tag="ACG8KQ_002028"
     CDS             2309..3259
                     /gene="manA"
                     /locus_tag="ACG8KQ_002028"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9104951.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3353..5056
                     /locus_tag="ACG8KQ_002029"
     CDS             3353..5056
                     /locus_tag="ACG8KQ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9104952.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5058..7091
                     /locus_tag="ACG8KQ_002030"
     CDS             5058..7091
                     /locus_tag="ACG8KQ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP9104953.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7102..7542
                     /locus_tag="ACG8KQ_002031"
     CDS             7102..7542
                     /locus_tag="ACG8KQ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9104954.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7742..8869)
                     /locus_tag="ACG8KQ_002032"
     CDS             complement(7742..8869)
                     /locus_tag="ACG8KQ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104955.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8872..9549)
                     /locus_tag="ACG8KQ_002033"
     CDS             complement(8872..9549)
                     /locus_tag="ACG8KQ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9104956.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9813..11579
                     /locus_tag="ACG8KQ_002034"
     CDS             9813..11579
                     /locus_tag="ACG8KQ_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9104957.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11594..12031
                     /locus_tag="ACG8KQ_002035"
     CDS             11594..12031
                     /locus_tag="ACG8KQ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9104958.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12044..13195
                     /locus_tag="ACG8KQ_002036"
     CDS             12044..13195
                     /locus_tag="ACG8KQ_002036"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9104959.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4493 a         2300 c         2732 g         3707 t
ORIGIN      
        1 cagctttttc acaacctctt tttataagag tttttactca ttaatttaca aaaaacaagc
       61 aaaatgcgaa ttttctcaca ttttttttaa caaaatcaaa gttattgctt gaaagaagaa
      121 gaatccatcg ttaaaataaa gcttgacgta gaggggagca agaaatatga aagattttca
      181 attgggggct gccacctatt taaaaagtgt agccattcgg gtgaaagacc gagataaaat
      241 gattgatttt tacaagaacg ttgttggctt tgatttgaaa agagaagaaa acgaattagc
      301 cattttagga acagaagaag ctggaagtga aatgttgctg ctggaagaaa gtcctagagc
      361 gaatgatcac atgggggtga tcaaaaagct gaaccgtttt tcattgatta tccctagcac
      421 agaagaaatg gcagatatcc tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc
      481 tttagaagat catggacgta tggggatttt gctcgtagac ccagaagaga atgaattgga
      541 aatttaccat gaagagaata agaacgacca aagagaaatt ccagaaacaa tggatcaaga
      601 agcattatta agaaaatctg aagaaaaatt cagtaaacta tcaacaggtt cttattttga
      661 aaaaatccat ttaaatgtta ctaacttaac aaaagaacgc caatttctta atcatgtact
      721 tggtttgaag gtacataatg aagaagaggg catccatgta ttgaatgaag gagatttcca
      781 tgttggtttg acagaagcta gcggaggcgc aattgatttg cctacagatg aagtgattgg
      841 acttgatttt ctgaaattca tgatcgatac tgaaagcatg gaaggactga ttgctcactt
      901 gcagcaacta tcagttgatt tcttcttaga caaaaagcac acagtactca caatctatga
      961 tgctatagga atcgaatggt ggttcgtcag tgagccaaaa gaaaagaata aaactgacat
     1021 tcgatgcgac gcttaacttt ttctttctta acgattgtcg catgtctcgt cattttatga
     1081 acggtcatac tatcgcttat tctagagata gaaagagaaa atagtatacc gggggaaact
     1141 gcgcggtttt tcgcgcagtt tttttgtgta aaaaaggttg attcctcata gattaagctt
     1201 taagcttgga ctgtaagaat ctgatagaaa agcaggagaa aaagatgtat ccacacacga
     1261 aacagcttat tttgaaaaag atggaagaag gattttatcc aggtgtagca gtcgccttca
     1321 taaaagaaaa tcaagtagaa acatttacag ccggctttgc ccaagtcgtt cctgaaaagc
     1381 agctgatgac agaagatctg ttgtttgatg cagcctcact gacgaaagtc gttgcaacca
     1441 cttcattagt tttacgtcta ttagaagatg gaaagataga actcgatcaa ccgcttcata
     1501 aatatcttcc tgaatttgaa aatcagacaa tcactttgcg tcatttactg acacatactt
     1561 ctgatatcaa aacatggata ccaaacagag ataagctatc tcaggaagaa ttgatcgccg
     1621 cttatttgaa gctagaacca ggtgagcaac taggaaaaga agtaaaatat acagatgccg
     1681 gtacgattct tcttgggttg atgctagagg aaatttacca agaaactgca gtggaaatct
     1741 ttcaagaaca agtattggaa ccattaggaa tgatgaacag cttatttctt ccttttcctt
     1801 ccaaaaaaat cgttcccaca gaacaactgc cttcgggtga ggttttgcgt ggacaaactc
     1861 atgatccgaa agcaagagtt ttggctgaac atgcaggtaa cgcgggtctc tttattgatt
     1921 tgaaagacag cattactttt gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc
     1981 gttttttaga agaagagacg attgattctt tactgcaaga tcaaacaccg aaaaaaaatg
     2041 gctatcgttc tttaggatgg gacttaaaaa aggatgtaga agacggtcat gcgattcttt
     2101 ttcatactgg ttatacaggt acgtttttct tgattgatat aaaaacacaa gaagcgttga
     2161 tatttttgtc aaatcgcgta catcctgtcg atcatcgtgc ggagtatctc atatgtcgag
     2221 atgaaattgt tgaaaacttt ttgaaagaga aaacggtatt accgaaataa tgctataata
     2281 gctatgaaaa atgagaggag tttgaattat gcaggaacct atgtttttaa aaccagtttt
     2341 tcaagaaaaa atttggggag gaagtcgttt acgttcagtc tttggttttg atatccctaa
     2401 cgataaaatc ggagaagatt gggcgatcag tgcgcatcct catggtgtca gtgtagtaga
     2461 aaatggcgaa ttcaaaggga aaagattaga tgaactatgg tcagaacaca aggaattgtt
     2521 tggccatcct tcagaaccag tgtttccatt attgatcaag atcttagacg cagaagatga
     2581 tctatctgta caagtacatc cagatgatgc ctatgggatg aaacatgaag gtgaattagg
     2641 aaaaactgaa tgctggtata tcatcgatgc tgaacctggt gcagaaatca tctatggtca
     2701 tcatgcaaag acaagagaag aactagctga gatgatcaaa gatgggcgct gggatgacct
     2761 tttgaagaaa gtaccagtta aaaaaggtga tttcttttat gtaccaagcg gaacgatcca
     2821 tgctattggt aaaggaatca tgattttaga aacacagcaa agttctgaca cgacttaccg
     2881 cgtgtatgac tacgacagaa aagatgcgca aggacaaaca agagagctgc atatccaaca
     2941 atcgatcgat gtaacaactg tccctgcgaa aacaccagaa ttacaaatca aagaagtccg
     3001 aaaagggaat tcatcgatcg tcacttattt agaaactgag tttttcaatg tatatgagtg
     3061 ggatattaaa ggaatcacat ctttcaaaaa acaagcccct tatacattga tgacggtaat
     3121 cgaaggagca ggagaccttg tcgttgacgg aaaaacatat ccattagaaa aaggaacaag
     3181 ctgtattatt ccaaatggcg tttctgagtg ggcggtccaa ggtgaactag caatcattgc
     3241 gactgaacca ggcaaatagt tcccagatag ctgaaagcag cagacggttt ttcgtttgct
     3301 gctttttcta cataaaatcc gtccatcaat aaatagaagt atggtgaaaa agatggaata
     3361 caaatggaaa gcaaacaaca gcagtcgcgt gtctatgaaa caactgagaa aagctggaca
     3421 tcacttaaat gaaatgatca tgccaaatct aagtctgttt atcgcttggg gaatcttgtc
     3481 attagcaaca aaaggtgtgt ctggcgattt gcacaccaca ttagaagagg tatgccattg
     3541 gatgatccag ctgcttttac ccatcttgat cagttattca ggcgggaaat cggtaggggg
     3601 acaaaaaggg gcagtcgccg gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc
     3661 tccacaaatc gtaggagcga tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt
     3721 tgtttctaga ttccacgtga aattcaaggc tggatatgaa atgcttgtca gcaacctact
     3781 agtaggactg actggtagcg ttatttgtat aattggaatc gtttttattg aaccagtttt
     3841 aaatagtgtt cacttgttgc tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt
     3901 accacttgtc agtctgatct tagaacctct aaaagtacta tttttcaata atgcaatcaa
     3961 tcatggtgta ttgacacctt taggaatcga attctcacaa acagtcgggt actcttcctt
     4021 atttttgatg gaggcaaatc ctggacctgg tttagggatc ttactttcta tcctttgttt
     4081 tgcagaaaaa caggaaaaag taaatgcgag tggagcattg atgatccatg cgattggcgg
     4141 gattcacgaa atctattttc catttgtctt gctgcgaccg tatctatttt tagctgtaat
     4201 ggctggtggg gcaagcggca cgttgatatt tcaatggtta gatacgggat tagctgcacc
     4261 tgtatcgcct gggtcactac tcgtgatttt tgccaatgcg ccttatccac aactagctgg
     4321 ggtaagtata gggatagcaa tcagtacatt tgtgaccttc atttgtgcag cgatactatt
     4381 gaaattacca aataaagaaa aagagaacaa aacggtcaaa gaaaggggag aaatcttgga
     4441 accatctatc attcattccg tcatttttgc ttgcgactca ggtatgggat caagtgctat
     4501 gggagccgcc ctgctgaaaa agaaattagc agcagaaaaa atcgatcttt ctgtcggata
     4561 cagctctatc tatgaattga ataaccaagt aaatcagctg gttatcgtgc aacatgaact
     4621 agcgggtctt gcaagagaaa aagtacctca aagtcaaatc tatgtactgg atcacttttt
     4681 ggaaacagat atcttgctgt cacgaataaa agaagcgatc caatttccaa agcgggaaga
     4741 aaaaaaagag atagcagata tcacagaaca aaaaaagtcg cctgctaata ttgtattttt
     4801 gtataaaaac aacagacgcg gctcacaaac tatgggcatg acgattttaa gtcaattagc
     4861 aaaaaaagcg aaaaaacagt ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca
     4921 agaagaaacg atttatatcg cacaaagaga actagctgta actgaacagc tagaagaaaa
     4981 gatccctcgg ctagttctag ttgaacattg ggtagctacg aatgaatacg aaaaactcgt
     5041 gaagggagaa atataagatg tatttatcgc ccagagaaaa acaattatta gcagaatttc
     5101 tcagtagccc gtcacctgta tctatccaaa aaatgatgaa tttattgaag gtcagtaaac
     5161 ggactattta ccgcgaatta gataatcttg aagtctcatt gaaatcggta aatgcttctt
     5221 tgaaaaaagc agctagaggc tcatttgtca ttgaggcatc agaagaagta ttagagcaat
     5281 taaaagaaaa tacagatggg aaaagtaatg acttatccac tgctaaacga caacacgcga
     5341 ttttagccga gttgttactg acgacagagc cgttgagtct tgctcatttc atggaatcat
     5401 atcgcatcag taataccact ttttatgcag atatcaaaca gttggaagaa agtattcgcc
     5461 aactgccttt ggaaatcatt cggaaccagg gatacgagat cgaaggaccg gaaaaatacc
     5521 gccggcttct gacagcgaat gtattagaat tagaaatcaa tgaatatgaa ctttttcata
     5581 gtatttcttt cgatgcatca ctgaattatt tttttcgatt cgttgatccg caacatctat
     5641 ctcttgcacg caaagtaatc ggggaagaat tgatccaaaa aaagaccaat ctaagtgata
     5701 gaaagcttga acatttggtt ttgatgctga cattgacgat ggagcgtgtg actaaaaatc
     5761 atttactgac aaatgagaca tataatagtc tggcaaacaa agaattacta cagacagcta
     5821 aacgattgtt ttcacggatc gcttctgaga caaaacaact ttatccagta aatgagatcg
     5881 tcttttttgc aggattatta aatgacttca cgaactcatt tgatcagaat ttctttgaag
     5941 agacgttcga tacacagctt gcttatttag taaaacaact gattcaagaa gtaagtgaag
     6001 agacgaatgt ccatttttat gaagatgaaa ctttgtacaa aatgctgttg actcatttat
     6061 ctggtgtgtt ttcaagagcc gttttacaag aagagacact gaccaatccg attttagaaa
     6121 aaatcatggt tcaatacgag gaagttgcga atgcaatccg taaagcagta gagcggattt
     6181 ttcaagaaaa aaaattatcc gaagaagaga tcgcttatat ggtccttcat tttgcgaatt
     6241 cattggaacg aagtccgaaa gaaagcaata tcagtattgc aggattttcg ccaagtggat
     6301 tagcttctac tagcatgttg gaactacgat tgaaaaggta ttttcccttc atccaacaaa
     6361 ttcatttcta tcggattgct gatttaaaaa aaatcgattt acaagaagaa tatgatgttg
     6421 ttatttctac atctatttta ccaggatacg gtggaaaata tattttagtc tccccgctac
     6481 tgctagaaga tgaagtcaag cagctaaaag aagctttttc gcatattgag aagaaaaaaa
     6541 ggcaaaaaat tacgtataac agcaataacg agtggatgat agaagatact tatgaggaaa
     6601 caatgcattt catggatcaa atcaatcaat tactcggtcg tttttttatc caagaactgg
     6661 ataatcctga gacgatttcc gaaacgattc gcagcttatt tccttatttt cctgaaggac
     6721 tagtaacgga tgaagaggtg gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta
     6781 tcggtattcc tcatacaaat atggggttat tccatacatc cagctcattg atttctaaac
     6841 cgatcttttg tattttcaat ttgaaacatc cgttgaccat tgaagggatg gatagacagc
     6901 cagtcgattt gtcccgtatg ttagttatgc tggcgccttc gccaatccaa gaaaaagacg
     6961 ggattctttt aggaaagatc agtggagcaa tcatcatgaa tgacttgaac accgagatct
     7021 tccgctcagg aaataaagaa atcgtttatc agttgttagc gaaaatcctg attgaagaaa
     7081 taaagaaatg aaggtgaaaa gatgatacag ctaccagcag aaaaaatatt gctcgatcaa
     7141 tcctatcaaa attggaaaga agcgttgacc gagatcggcc agtggatgac ccaacaaggg
     7201 ggaatcaaac cggcatatat ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat
     7261 atcgggaatt tcgtagcctt gccacatggg actgacaaag atgaagaagt agtagaagaa
     7321 ggaatctatt tagtccaagt gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa
     7381 atcgcgacac tcttatttgt agtggcttta aaaaaagaac atcagttagc ggtgctccaa
     7441 gatttagcct ttttctgttc agacatcgac caagtaatgg cgctcagtga tgcgaaaacc
     7501 attgaagaag tccaaatgat ttttgaacat ggacatatat aaccaatgtt cacagtaaaa
     7561 aaagctgcct tgcatcaagt aataacttca accaagataa aaataagagg agctattttt
     7621 gttgggaggt tcttattact gcggatgcaa gcttttgaaa actatttatt ctagaaaacc
     7681 gtttatttga aataggggac ggtgacaagg tttttgtcac cgcccctatt atttttgtgt
     7741 tttaatagct cttttttata tagatagtgc ccatacctat gaataataaa acgatactcc
     7801 atataccaag tgtcaagata ccattcatga aagtaacctg ttctgaagct agtggcgaga
     7861 aaatatctcc tcctatgatc acattttgaa aatcaacata agaagttggc aagtaatgac
     7921 tgattgattc tatccatcca atcgaagaaa acaagcgata ctctgctaag aaaagacaaa
     7981 gaagtgtacc taaaagattg actagagaat gttcaaataa agcagataga agaaaactga
     8041 ttgtacttaa aaagagcacc cacaacaaca ggaacagaaa gctctggaaa agatactcat
     8101 acatcggaag gattcgtatc tcctgaccaa tgatcgttcc tacaggataa cggaagtctc
     8161 ccaaaccatt tttgattatt actagtatag agacgatcaa aaatggcatc acgagactta
     8221 gcatagctgc accataataa atggtaaaac ggatgctatg tttctttacc atactatccg
     8281 gtaatagatt gactagagag attacctgct ttcttttttc ataagagatg atggaagaaa
     8341 gaaacaaggc ggttatacat aaaatcaaaa aactagaaat cattcctgaa aaaatatttt
     8401 cgtagtaatt tgctaaaggg agatgcgctg gcaagtcaaa aatagaatat ctttggatct
     8461 gatgttcttt catataacgg agcaactcga cacgtttttg ttgttcaatg attggaattc
     8521 cttgtctttg accagaaatc agtcgatcca gtatatcttt ttcataatga taagttgctt
     8581 cgaccatttg ctggacattc cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt
     8641 tgcttttctc taaactttcc acaaattctt tctcattctc atcttctttc aacgtttcaa
     8701 tcaaacgttg catctcttca tagttatgat tcagttcatc gaattctgta taagcaagtt
     8761 tatgatcaaa aaaaagtggg aatgcaaaga gcacgaccaa caatagagga aataaaaagt
     8821 ttactttgtt ttttagtaag agattgcgtt caaatgtaag taaagacata gttacacctc
     8881 atataattta cgataaatag ctaaaatctc ttccatattt gaggattctg tgacgatttc
     8941 tccatttttt agaaaacatg ctttagaaca aatcgattgg atattcgcga tagcgtggga
     9001 agaaagaaga atcgtcttcc cctctttctg ccattgtctg atgaggctat tcaagatatc
     9061 aatacttcct gggtctaatc cgttcaacgg ttcatcgaat aacatgacag atgaatcact
     9121 gatcaagtaa gcagcaagca agacatgttg tttcattcct agagacattt ttccaatcgg
     9181 caaagtaagg tactcagtca tcttcaatcg ttggaaaaca gtttggcatt ctactttgct
     9241 ctgccaatag tatttgatca aagtcaaata atcaacagcc gatagatagt cggataactg
     9301 attcgttgat tctagaaaaa agaaagaccg gcgataatct gtataagcag taggagagat
     9361 gttgtgaagg gacaattgat cataagttgt tggcagcagt tgggcaatgc cattcagcaa
     9421 agttgttttg ccaaatccgt tcggggcaat caaagcagct acttctcctg attctactaa
     9481 aagattcaat tgtcgaatca cacgtttttt accacgtttc atgttcatgt ttttgatcag
     9541 taatgtcata gataggcctc caatataaaa tttaataaca taatagaatg gaaaacatct
     9601 aaattaaatg ctaacaaaag ttattgtttg catattataa aaaaattaaa ataacagttt
     9661 tatttaaaat tgaagttttc tgataccgaa atctttttat tttgctgaca tactcaaacc
     9721 tttggcacaa agtttcgtgc caaagtacga ctatttctct ttttagtaag cggatacaat
     9781 tagagtatca aaaggagaga gggagagaca aaatggaacg gacgattccc aaaaacaaca
     9841 tttcgctaaa agcgagggta caaaaattag gcagtacttt atcaagcatg gtcatgccaa
     9901 atattggagc actgattgcc tggggtgtat taacggctct atttatccca gatggttatt
     9961 taccaaatga ggctttggct accatggtaa gcccaatgct aacttatctg attcctttat
    10021 tgatcggtta tactggcgga aaagtaattg ctggtgaccg cggatcagtt gttggggcaa
    10081 ttgcaacgat gggtgtgatt gtcggaacag atattccaat gatgctaggc gcgatgatca
    10141 tgggacctct tggtggatat gccatcaaga aatttgatca gctattccaa aaacggatca
    10201 aatccggttt tgaaatgcta gtcaataatt tttcagcagg tcttatcggt tttggattag
    10261 cgttattagg tttttctgcg attggtccag ttgtcgatgc attgactcaa gcaatggcaa
    10321 aaggtgtaga gatcatttta tctgcccatt tgattccatt gacaagtatt tttattgaac
    10381 cagcaaaaat cttatttttg aataatgcaa tcaatcatgg gattttgaca ccattgggaa
    10441 ctgaacaagt agcagaggct ggaaaatcta ttctattctt gcttgaagca aatcctggtc
    10501 caggacttgg cgtattactt gcttttactt tgtttggcaa aggagcagca aaatcttcag
    10561 caccaggagc catgatcatt cactttttag gcgggatcca tgaaatctat tttccttacg
    10621 ttatgatgaa accgttatta ttcttatcag ttataggcgg aggaatgtca ggaagtttca
    10681 ttttccaatt gatggatgct ggactaagag cgccagcttc tccagggtcg atcattgcga
    10741 ttctagcgat gacaccgttc aattcttacc ttcctgttat tttgggcgtg attgttgccg
    10801 cagctgtttc gtttggtatt tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg
    10861 aagacttcga aaaatctgtt caagagacaa aggcagctaa gcaagcttcc aaaggtgccc
    10921 aaggtattcc tcaaggagct acattctctg gcgtacaaaa aatcattttt gcttgtgatg
    10981 caggaatggg atcaagtgcg atgggcgctt cgatcttgag aaaaaaagta caggaagcag
    11041 acttgcctca aacggtgacc aatcaagcaa tcagccaact gacagatgac tcgaatacat
    11101 tgatcgttac tcaggtcgaa ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt
    11161 ttgtctcagt agaaaacttt ttgaattcac ctcgatatga tgaaatcatt caagaattaa
    11221 gcagcaagca gaaaacatca gtttctgaaa ttgagaatga gccagcagaa gagtttcttt
    11281 cagcaaacat caaacaaatc aaagagattc tacttgtcca cgatgatcgt tccggatcag
    11341 caacaatggg gatgaccgtg ctcaaagata tattagacaa gaaccattta actatcccgt
    11401 taagaaaagt caatatccaa gatttaaaag atgaacctga ttcactgatc gtagccaaag
    11461 aaagtttagc ggaaaaagta cagatggctg cacctcaatc cattcattta tcagtcaaca
    11521 gcatggtcaa tccgcaaaaa tacgaatcga ttgcacaata tttgaaacag acagcttaag
    11581 ggaggaaaac agcatgcaaa gtttgccatt cgacatgatc gagctcaatc gatcttttaa
    11641 aaccaaagaa gaagcgatcc gctattgcgg tcagatgctt gtagaagcag ggggcgttga
    11701 tgaggaatat atcgatgcca tggtagatag agacaaaatg ttatctgttt atatggggaa
    11761 ttttatcgcg attcctcatg gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat
    11821 ctgtgtcgtt caagtaccag atggtgtaga ttttggtacc gaagaagaag aaaagattgc
    11881 gactgttttg ttcggtattg caggagtcgg agatgagcat ttgcaacttg tacagcaaat
    11941 cgcactttat tgcagtgata tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga
    12001 agaagtaacg gaaaatctag caattgctta aggagagaga aaaatgagag cagtacattt
    12061 tggtgcagga aatatcggtc gaggatttat tggagaggtt ttggcagcta atggatttga
    12121 gattactttc gtagatgtga atcacacgat cattgatgct ttaaatgaaa gaaaagaata
    12181 cgaaatcgaa ttagcagatg aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat
    12241 caacaatcaa acaaatccag aaaaagtaat ccaagaaatc gctgaagcag atttagttac
    12301 taccgccatt ggcccgaata tcttaccttt tattgctgag ttgattgcaa aaggtatcca
    12361 agaaagagaa aaggaaggaa gcacaacatc tttagatatc attgcctgtg aaaatatgat
    12421 tggcggttca gcatttctag aaaaagaagt gtataaatat cttccggaaa catcatttac
    12481 tgacaaatat atcggctttc caaatgcagc agtcgatcga atcgtcccat tacaacacca
    12541 tgaagatcct ttattcgtcc aagtggaacc tttcaaagaa tgggtcattg acgatagcca
    12601 aagaaaaaat aaaaaaatcc aattaaaagg ggttttatat gtagatgatc tagaaccata
    12661 tatcgaacgc aaactattta gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc
    12721 cttgttaggt taccagacca ttgatgaagc aatgcaagat gcgttagttg tggcacaatt
    12781 aaaatcagtt ttgcaggaaa caggaagttt gctgattgcc aaatgggggt ttgatgcaga
    12841 gcagcatcat gcgtatattg aaaaaatcat tcatcgcttc caaaacaaaa atatctctga
    12901 tgcgatcaca cgtgtcgctc ggacaccatt acgtaagtta ggttatcaag aaagatttac
    12961 acgtccagtg agagaactac aagaacataa cttgacttgc ccacatttga cggcaacaat
    13021 ggggattatt tttaattact atgatcctga agatgaacaa agccgtcaat tgcatgaaat
    13081 gaagatccat gaaaacttgg aacaattgat tcaagaagtc acaggaatca atgatccgaa
    13141 aacaatcggg aatatcaagc aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag
    13201 gatacgaaaa aggggaatgt gacgaaattt tt
//
LOCUS       DAZKCE010000078        13183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000078 DAZKCE010000000
VERSION     DAZKCE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.079"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..293)
                     /locus_tag="ACG8KQ_002037"
     tRNA            complement(221..293)
                     /locus_tag="ACG8KQ_002037"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Lys,seq:ctt)
     gene            674..1243
                     /locus_tag="ACG8KQ_002038"
     CDS             674..1243
                     /locus_tag="ACG8KQ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9104960.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1230..2090
                     /locus_tag="ACG8KQ_002039"
     CDS             1230..2090
                     /locus_tag="ACG8KQ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9104961.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2280..2984
                     /gene="yycF"
                     /locus_tag="ACG8KQ_002040"
     CDS             2280..2984
                     /gene="yycF"
                     /locus_tag="ACG8KQ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9104962.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2989..4824
                     /gene="walK"
                     /locus_tag="ACG8KQ_002041"
     CDS             2989..4824
                     /gene="walK"
                     /locus_tag="ACG8KQ_002041"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9104963.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4821..6137
                     /gene="yycH"
                     /locus_tag="ACG8KQ_002042"
     CDS             4821..6137
                     /gene="yycH"
                     /locus_tag="ACG8KQ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9104964.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6138..7007
                     /locus_tag="ACG8KQ_002043"
     CDS             6138..7007
                     /locus_tag="ACG8KQ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9104965.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7062..7871
                     /locus_tag="ACG8KQ_002044"
     CDS             7062..7871
                     /locus_tag="ACG8KQ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9104966.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7974..8603)
                     /locus_tag="ACG8KQ_002045"
     CDS             complement(7974..8603)
                     /locus_tag="ACG8KQ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9104967.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8773..10065
                     /locus_tag="ACG8KQ_002046"
     CDS             8773..10065
                     /locus_tag="ACG8KQ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9104968.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10141..10803)
                     /locus_tag="ACG8KQ_002047"
     CDS             complement(10141..10803)
                     /locus_tag="ACG8KQ_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9104969.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11028..11312
                     /gene="groES"
                     /locus_tag="ACG8KQ_002048"
     CDS             11028..11312
                     /gene="groES"
                     /locus_tag="ACG8KQ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9104970.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11363..12988
                     /gene="groL"
                     /locus_tag="ACG8KQ_002049"
     CDS             11363..12988
                     /gene="groL"
                     /locus_tag="ACG8KQ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9104971.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4386 a         2277 c         2841 g         3679 t
ORIGIN      
        1 gcagcatgat tttgcaaagc ttctcgaatt attgttatat tagaaacttt actgttcata
       61 attatgcttc ttttttaact caaagtccaa agggctacag tggactttga gttaaaaata
      121 aaaagcccta gttattttga aaaataaaaa aagaacagcg taaatgtttt cgaaatgctt
      181 tcgcaccaac atttacctgt tccgtgggtt tggcacccaa tgggccgtga ggggctcgaa
      241 cccgcgaccc gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa
      301 aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat
      361 cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta
      421 ttatataagc ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc
      481 attttttact cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac
      541 aagaaggctt agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag
      601 ttaaagcaac tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa
      661 aggagcggtt atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga
      721 aacattccgt ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt
      781 cagaacgtta gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct
      841 tacctcttct atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa
      901 aagatataac aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt
      961 gactattcct gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga
     1021 agcctataat ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc
     1081 acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt
     1141 aagagcggca actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga
     1201 aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag
     1261 attcaggtac ggatgttcca gaagaattga taaaaaaata cgagatgtat atgatcccat
     1321 tgaaaattat ttataaagat caggtatata cagataaaat tgacattacc cctgaagaga
     1381 tctatcaacg tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca
     1441 cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt
     1501 ccagtggatt gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat
     1561 tggaagcata tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc
     1621 aagcagcaaa atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg
     1681 agttagtcga aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag
     1741 gtggacggat cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca
     1801 tctcatgtaa tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa
     1861 gtttggataa aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact
     1921 tagctgttgc ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa
     1981 gagagttccc acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc
     2041 acactggtcc gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa
     2101 ggaaacactg aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa
     2161 taaagtattt tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca
     2221 aaagcagaaa tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta
     2281 tgaaaaaagt tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt
     2341 tagccaaaga aggctatgac gtttatactg catatgatgg ggaagaagcc ttggaaaaag
     2401 ttgctgaggt agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct
     2461 tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca
     2521 aagattctga aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca
     2581 agccattttc caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca
     2641 cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga
     2701 tccatccaga agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg
     2761 aattcgaact actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt
     2821 tattacaaac ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg
     2881 tacgtcgttt gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac
     2941 gtcgtggtgt cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa
     3001 gtccgctttt ttcgatcagt caattttaag atcgctatta cctttattct gattttattg
     3061 atctcgatcg aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat
     3121 acatttatta aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa
     3181 ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt
     3241 gaaaacagtg cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc
     3301 cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat
     3361 atcaatgatt tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat
     3421 atcaatgttc aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc
     3481 aaaagtaata tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact
     3541 gcctcactaa ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt
     3601 acacagccga tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt
     3661 cggaaagtca aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag
     3721 ttagctgaac gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat
     3781 agcgtgctat cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt
     3841 acgatcaatg atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa
     3901 tcgattttga cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat
     3961 ccaaacgaaa tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg
     4021 cgagtagact tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc
     4081 ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat
     4141 gtttcccatg aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc
     4201 gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa
     4261 acagaccgaa tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt
     4321 aatacccagc ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac
     4381 cggtttgata tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg
     4441 caaagagatc tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc
     4501 atgaataacg cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa
     4561 acgcataata acgtgatttt aagtatcacc gatcaaggat tggggattcc taaaaaagat
     4621 ctgaaccgag tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt
     4681 gggacaggac ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt
     4741 tgggcagaga gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct
     4801 tatgaggagg aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt
     4861 tgatggttgc gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa
     4921 gttcaatcaa cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa
     4981 cttccgaagt ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa
     5041 ccaacagtga aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa
     5101 agctttctga agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact
     5161 ctggttttga gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg
     5221 atttagattt agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa
     5281 tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat
     5341 atgaagcaaa tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca
     5401 caaaatggac agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac
     5461 attcgatcaa actgaagaag tatagttata tcctgtcaca gcaaccctat acacttttta
     5521 gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga
     5581 ttttttacgg tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc
     5641 gtggagagct gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata
     5701 ttagcaaatt aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga
     5761 tcgattatcg gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga
     5821 ttggtgtgga agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt
     5881 tgaatacgat tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg
     5941 aaatcatcca gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg
     6001 ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat
     6061 ggtatgtgaa atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg
     6121 gattggaggc gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt
     6181 tagggttgaa tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg
     6241 tcattcgctc taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt
     6301 acacggggaa gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca
     6361 atcttagcac ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg
     6421 gtgtgttttt agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga
     6481 acgtgatcga tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca
     6541 atattttatt cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac
     6601 cactagaaat ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa
     6661 aaatcgttct ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga
     6721 cgcatttatc tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag
     6781 caatcaatac attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc
     6841 agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc
     6901 atgtagcgat cgaaacaagc gataaagtgg tccaaattga gagcgtcaac gctttcagca
     6961 atacgatcgt gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata
     7021 atgaaaaaag tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag
     7081 cttttaaaat cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg
     7141 aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg
     7201 ctcaaattga tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag
     7261 atcatataca tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc
     7321 agcagacttg ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc
     7381 tatttgagat ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct
     7441 cccatgacgc agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca
     7501 tgttgacgga caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg
     7561 gttatttgat ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga
     7621 atttaaaaca gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg
     7681 tcatgactga tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg
     7741 aaaacaatat gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt
     7801 ttggcgttgg acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat
     7861 tttcgattta aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg
     7921 gttggtttta tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga
     7981 ctgactacac tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca
     8041 atcgcgtaat ctgccatgct gatcgtactg tcgctagcag cgtttctttg taaataatca
     8101 tcacttaaca aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg
     8161 atataagtcc aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg
     8221 aaagctgttg gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt
     8281 tctgtatcta agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct
     8341 tcaagcatct caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga
     8401 gcgttgaaag catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat
     8461 aaatcctttt gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc
     8521 gtcacgtcta aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta
     8581 gctccaatga gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca
     8641 gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat
     8701 ggttcgtgat cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga
     8761 aggagataag aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt
     8821 gtgaatctta gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt
     8881 attgttggct cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg
     8941 atagacaaaa caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg
     9001 atagcctttt ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat
     9061 cgcttaccag tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat
     9121 actcagatag atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg
     9181 ctcatcatcg gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga
     9241 gagaagatac ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata
     9301 gtcactttta cgcttatcgg ttcagcggtt tttcaacatt tgttcagaat gactgttgtt
     9361 caaggagtat ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt
     9421 gtgcttttat taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg
     9481 atggtcaatg ggttggttag tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc
     9541 ttagcctcag gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt
     9601 ctcgatcagt tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg
     9661 acggtttttg cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc
     9721 tttttcaaca tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg
     9781 gctgttcctt ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat
     9841 tttggattcg ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc
     9901 tcgggtttct tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt
     9961 agttttttca tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa
    10021 agacagacaa aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc
    10081 ctgtcgcttt agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt
    10141 ttatactagc cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac
    10201 gtgtgtgatc atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa
    10261 acctaggaaa aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga
    10321 gctgatcact gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg
    10381 gcggaatacg aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg
    10441 tgcttccatg atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa
    10501 caatacagat tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac
    10561 caatccataa ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa
    10621 gggttcacgt tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac
    10681 tgcagttgct gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa
    10741 gggtgagata aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga
    10801 catgtaattc ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt
    10861 tcaattatat cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta
    10921 aaagcttgca aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg
    10981 ttaagagtgc taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat
    11041 taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg
    11101 ttttagcttc tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag
    11161 gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta
    11221 tgttcgaaaa atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg
    11281 caggaaaaga tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa
    11341 taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc
    11401 agctatgtta cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa
    11461 aggccgaaat gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt
    11521 gacgatcgca aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt
    11581 atcagaagta gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt
    11641 tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct
    11701 aggcattcgt cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat
    11761 ctcaactgtt gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc
    11821 tgataaagtt ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat
    11881 cacaatcgaa gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt
    11941 tgatcgtggt tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct
    12001 agaaaaccca tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc
    12061 attgttagaa caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga
    12121 tggtgaagca ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc
    12181 tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt
    12241 gacaggtggt acagtaatta ctgacgatct aggtttagaa ttgaaagatg caacaatcga
    12301 aaacttagga aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg
    12361 ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga
    12421 aacaacttct gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg
    12481 tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg
    12541 cattgaagat gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg
    12601 tggtacagct ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt
    12661 ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga
    12721 aaacgctggt tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac
    12781 tggtttcaat gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc
    12841 aactaaagta acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac
    12901 gacagaagca gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga
    12961 tccatcaatg atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca
    13021 ttagtgaaag acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa
    13081 gaaattcacg gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt
    13141 gcttctgctt ccgctgttta tacgtgttta gagcgtgaga caa
//
LOCUS       DAZKCE010000079        12921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000079 DAZKCE010000000
VERSION     DAZKCE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.080"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8KQ_002050"
     CDS             133..3660
                     /locus_tag="ACG8KQ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9104972.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8KQ_002051"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8KQ_002051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9104973.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8KQ_002052"
     CDS             7448..7789
                     /locus_tag="ACG8KQ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9104974.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8KQ_002053"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8KQ_002053"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9104975.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8KQ_002054"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8KQ_002054"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9104976.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8KQ_002055"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8KQ_002055"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9104977.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4390 a         2244 c         2739 g         3548 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt a
//
LOCUS       DAZKCE010000080        12898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000080 DAZKCE010000000
VERSION     DAZKCE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.081"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..538)
                     /locus_tag="ACG8KQ_002056"
     CDS             complement(<1..538)
                     /locus_tag="ACG8KQ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9104978.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDK"
     gene            complement(535..1188)
                     /locus_tag="ACG8KQ_002057"
     CDS             complement(535..1188)
                     /locus_tag="ACG8KQ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9104979.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1476..1996
                     /locus_tag="ACG8KQ_002058"
                     /pseudo
     CDS             1476..1996
                     /locus_tag="ACG8KQ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1994..2713)
                     /locus_tag="ACG8KQ_002059"
                     /pseudo
     CDS             complement(1994..2713)
                     /locus_tag="ACG8KQ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2664..2873)
                     /locus_tag="ACG8KQ_002060"
     CDS             complement(2664..2873)
                     /locus_tag="ACG8KQ_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104980.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2855..2923)
                     /locus_tag="ACG8KQ_002061"
                     /pseudo
     CDS             complement(2855..2923)
                     /locus_tag="ACG8KQ_002061"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3150..4423
                     /locus_tag="ACG8KQ_002062"
                     /pseudo
     CDS             3150..4423
                     /locus_tag="ACG8KQ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4530..5561)
                     /locus_tag="ACG8KQ_002063"
     CDS             complement(4530..5561)
                     /locus_tag="ACG8KQ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9104981.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5568..6398)
                     /locus_tag="ACG8KQ_002064"
     CDS             complement(5568..6398)
                     /locus_tag="ACG8KQ_002064"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104982.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6395..7210)
                     /locus_tag="ACG8KQ_002065"
     CDS             complement(6395..7210)
                     /locus_tag="ACG8KQ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9104983.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7207..8031)
                     /locus_tag="ACG8KQ_002066"
     CDS             complement(7207..8031)
                     /locus_tag="ACG8KQ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFP9104984.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8021..9121)
                     /locus_tag="ACG8KQ_002067"
     CDS             complement(8021..9121)
                     /locus_tag="ACG8KQ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9104985.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9299..10084
                     /locus_tag="ACG8KQ_002068"
     CDS             9299..10084
                     /locus_tag="ACG8KQ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9104986.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10117..10500
                     /locus_tag="ACG8KQ_002069"
     CDS             10117..10500
                     /locus_tag="ACG8KQ_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9104987.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10576..10707
                     /locus_tag="ACG8KQ_002070"
     CDS             10576..10707
                     /locus_tag="ACG8KQ_002070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9104988.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10676..10912
                     /locus_tag="ACG8KQ_002071"
     CDS             10676..10912
                     /locus_tag="ACG8KQ_002071"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9104989.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10924..11010
                     /locus_tag="ACG8KQ_002072"
                     /pseudo
     CDS             10924..11010
                     /locus_tag="ACG8KQ_002072"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10990..11961)
                     /locus_tag="ACG8KQ_002073"
     CDS             complement(10990..11961)
                     /locus_tag="ACG8KQ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9104990.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12613..>12898
                     /locus_tag="ACG8KQ_002074"
     CDS             12613..>12898
                     /locus_tag="ACG8KQ_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9104991.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3878 a         2865 c         2323 g         3832 t
ORIGIN      
        1 ttttatcagt aacaattttt atttcttgta tattattttt tattgatttt gcataaatta
       61 aagaaataat agcagaaaat aaaattgata ttaataaaga atatggtaaa aatttaaggc
      121 ttaaatcttt cgcatctttt tgcatatcag ctgtagaaac aaattgtaga tttgtttttt
      181 taccatcatt aagctttatt tctctttctt caattatcaa agaattacta tcactttcta
      241 aattaacatt tatattatct ttgatttgta tttcattctt attatttttt tcttttatga
      301 atacttttat ttcactactc ttggaataaa actccaaagt ttgttctata tattttattt
      361 cttttccatt catattacta gaaatttcat ttgccatatt atgaatcttt tcttttctcg
      421 tctccaaata tgttttagga aagataaaaa aaattaatga atgaactaat ataattatta
      481 ttccaagaac agaaaatatt tgtatgaaca tttttggaaa tatctttaat tttttcattt
      541 tctctccaat ttatatccta catttcttat agtagttata cagtctaatt gtaatttttt
      601 tctaagttct tttatatata catctattac tcgatcataa ggagtttctt cgctatcttt
      661 ccatacataa tcaataattt gcattcttgt taacacttgt ccattattat ccaataaaca
      721 ttttaatact tctagttctt ttgcatttac atctatttct tcaccattta ttttagctgt
      781 atagctatta aaattaaccg atagatcctt atattcaaac ttctctaaat gtccataatt
      841 cttcttaatt aaagaatcta ttctagcttt taagaccggc aatgaaaatg gcttttctac
      901 atatccgtct actaaattag taaatgcatc aattttatat tcttcatcac taaatgcagt
      961 caagattaga attggtaaat tgcttttctt tctaatttct ttcaacactt ctaaaccatt
     1021 tataaaaggt atctgaatgt ctaagataac caaatttata tcgctattaa attttgacaa
     1081 ggcttctctt ccatctttag cttgaataac agtatatcca aattctgaaa gatattcact
     1141 tattccctct cttatcatat tatcatcttc aacagttaat attttcatat atacctccac
     1201 ttaagcctat acactagata ttatacagca tattatggct ctataatatc tagtgttggt
     1261 gacgccttct gttgccaata ctttttcttt tttttaataa atgcaaacag acaccttgaa
     1321 atcctttaga aaaaaggctt ctataatatt ttgtgttgtt ggaaaatcga aggcgatttt
     1381 ctactaacac tttttcttcg tttcaactca aaaagagact cacttttggt aaaatttagt
     1441 cgaccaaaac atcaccctac caaaggagtc gctctatgga taatcatact agaaaattac
     1501 tcggattaac agataaacat ttattttttg atgaagaatg gctgattgaa aaagaatata
     1561 aaggcgttca agcgcagttt attaaaggga gattggatga ctcaccttcc cattgtgaac
     1621 actgtggatc gattcgtatt atccgcaacg gttcctatac gacacgtact cagatgttga
     1681 aagtcaaaga aaagttgact attttagaat taaaacgtac ccgttttcta tgctatgatt
     1741 gtgggcagac cttttctgcg aaaacggact tagttgatga acatcatcaa ctaacaaagg
     1801 aactgaagca ggcgattctc atagaactgt acgaaaatca atcacgtaat ctgaaaatca
     1861 gaagaagtac cgtcggctca agcgatattg gaaactctta ctcaaagatt ccacgacatt
     1921 ggagccgctc aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat
     1981 cgtcgatgaa ccgttgtgca caatggtgtc ctgaacgtac agcgacacct tccatatcca
     2041 gagcagtcgc cacatcacgg ggatggatgc catccaaatt aaaagcaacc acgccagtat
     2101 gatgttcagg atcttgcggt ccgtaaatgg tgataccgcc gatcgcttgt aatttaggta
     2161 aaacgtaggc aaccagttct tgctcataac gatgaatatt ctccatacca atctcattca
     2221 agtaatcgat tgctcctgca atgttcggag taccagcttc aaatttccat ggcagctctt
     2281 tccatgaact atcgtaaaga ttgacaaaat ctatcatttc accgccgaat tcgactggct
     2341 ccatttgttc taacaattga cgtttgccat ataatacacc gattcctgtc ggcccgcaca
     2401 ttttatgccc gctaaaggcg aaaaaatctg catccaacgc ttgtacatca actgtcatat
     2461 gcggtgctgc ttgggcaccg actggactat cctcaactac aacagcctgt tcaaccctca
     2521 gccgtttcaa gcacaacaaa taaccttctg gatctggttt gccgacctga agatcatcga
     2581 tcgtcacaaa ggaatcaatt ttttccttaa tcatcggatg tgcttcaaaa aaatagtcta
     2641 gctcccgcag ttcagaattg agactactcc gattttccag tgctgctgat ccgctgctca
     2701 aaatgttcag catccactgg tctcccaacc gcttgactta tcccttcata aaaggacagt
     2761 tctgctgccg tcccaacacc ttgttgatag tctgcaaaag gaattttata gccgaactgc
     2821 tccagtaaat aggcttcagt aaatagtgat gaatactctc gtaaaacatc cattcactgt
     2881 caatcatcgt cccatcaaaa tcaaaaataa gtgctttggt catcttcttt cccccttacc
     2941 caaaacaagc aaaagctgtg tcctgctctc tctacagtat cagcataaaa actgccgtct
     3001 gaaaagtcaa aggtgaaaga aatgtggcgc tataatttct aggactgata gtaaatcatt
     3061 ctatcagtcc ttttgagcat aaaaaaacaa aacatacaga agctccgtta gaattgaatc
     3121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcttatc aaactattac
     3181 taggaatcaa agatggaaac attcaaatca ctcatgtcga gaagaaaaaa atacattcaa
     3241 tcaacagtat cgttgtttct gccacgctca ttaaaacgat tcagccctgt cctcagtgta
     3301 aacaggtgaa tcaacatggg ctgatcgtta agaacggaaa gaaaaaatcg ctggtccttc
     3361 tcaataagtg tgcgaatcaa cggacttatc ttgctttggc aaagcaacgg tacctctgtc
     3421 gtggctgtca cacctatttt acggcgaata cctacattgt tgaaaaaaac tgttttatcg
     3481 ccaaacaagt acattataaa atattggagg aattgactga aaaacaagca atgacgacga
     3541 tttctaaaca ttgcggcgtt tcttggtcaa ccgtttcgcg aacactcgct tctctgctcc
     3601 ccatgacaaa cgtgaaaata aattggttgc ctcgctgttc attggtagat gaatttcgtt
     3661 cattgaaaaa tcaagtcggt ccctattctt tttcttgtat ggatggcgat actgggaaat
     3721 tactggatat tcttccttca cgtaagaaga aagatctcat ttcttatttt atgcaatttg
     3781 aacgacgtgc acgtttaaac gtaaaaatac tcgtaacaga tatgaacgcc tcttatgctt
     3841 tgctgataaa agagtgcttt cctcatgcca aactcgttgt ggatcgcttt cacatcgtca
     3901 agcacctcat tcgcagtttt aaggatattt gactatgagt catgaaaagc ttcgaccgaa
     3961 acgatccaat acaagccaaa cactatcgac aagtgaaggc tttatctcga cttctgatca
     4021 aacgacagga cacgcttgtt tatgataaat ggacaaaatg gcgtaatttt ggttgggcct
     4081 atttaaccga aagtgaagtc gtagaacgtc tactttctac ttctgatgaa ctacgaatag
     4141 cttatgctta ctatcaagaa attcttcaag ctttttatga caaagaagcg gacatcttct
     4201 ttcagcttgt aaaaacaatg cccaagtcgg ttccaagaga actgcatcat ataaaaaaag
     4261 cgtttataaa ctatgaatcc ggcattcgtt tagccttaga gttgccttac tcaaatggca
     4321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg aatagcttac gggttaggat
     4381 gagaaccaga attttcttaa tcaatcatct gatcacttat taaacataaa aaaacatcta
     4441 aagcagaaaa tcaattctac cttagatgtt cttatatcac tctatcagtc ctatttgaca
     4501 aagagccaga aatgtttgcc gactatacac tagctttgtt gtgcaaaatg ctgagtatca
     4561 atttccacag ggattgctag aatatctttc agctgaatgt ataaggcatc gctaattttc
     4621 tcttcccgat cgttcaaccg atccactaaa atttctttgc cctccagcgt ttgaaaaaca
     4681 taccgttgaa tgttgcctaa aataaatgat tccttccata gaaccggaat cttaaccccc
     4741 tgaggaaccg gttcaaattg aatcagctcc ggtcttaaat aataaatgca gcgattgccc
     4801 ttagcggaaa actcgtgggt atactgattc agctcatacc attcgaaacg attatagttg
     4861 ccgataaatt ccgcaacaaa tcggctctga ggattctgat acaattcctt cgaggtggaa
     4921 acctgagcca cccgcccagc ttccatcaca aaaatccgat cactgattcg catcgcctct
     4981 tcttggtcat gagtcacaaa aatcatcgtc atccctagcg attgctggat agcgcgcaaa
     5041 tcccgctgca gctgttttct aattcgggca tccaaagcac ttaatggctc atccagcagc
     5101 aggactttcg gttcagtgac caatgagcga gccagtgcca cccgttgtcg ttgaccgcca
     5161 gaaagaaact ggacattttt atctgcctgc tccgtcatat ccaccaatga tagcatctca
     5221 aaaacctttt cctgaatcac ctcaggactc agcttcttta ccttcaaacc aaaggctaca
     5281 ttttcaaaaa cagtcatagt tggaaacaat gcatactgtt gaaaaacaaa gccgatattg
     5341 cgatccttcg taggcttatc ggcaatattc tcattgtcca gcacaatctc accgctcttt
     5401 ggttgttcca atcccgtgat acagcgcagc aacgtagact ttccgcaccc gctgggacct
     5461 agtaaagtaa ccagctcacc cttttcaatc tgaatttcat caatagctaa aatctgtttt
     5521 tcatcaaaaa acagattgag atcatgtatt tctaaaaaca ttttccgtca caactccttt
     5581 aagggcttct ttttcgactg cttctcttcc ctcgtctgct taagggacag ctgttgcttt
     5641 tgtcgataag tcagcataaa ggtagttaaa gcaattccca ccataaacag caaatacaaa
     5701 accatcacag cgctagccaa atggccgttt tcgttcatcc gccgcagcat atagatgcgt
     5761 accgtctgaa atcgaccgcc aatcaacagg ttcgtcagca tgtactcccc aaaaaccgaa
     5821 gaaaaaatca tcagagaaac tagggtcaca ccgatacgga tgtttggaaa cagcacctgg
     5881 ataataatcg tagacatcgg ggcgcccaag gtttctgctg catccactaa ttccttcaga
     5941 ttaattaagc gcatctggtt gttaagacta agatagacaa tcggcaacgc cgtaataaac
     6001 aatgcaccta ccaacactac aaacattggg ataccagttt cagcatacgt tcgaagcaaa
     6061 gccgttacca aaatcactcc tggtaaagca tacggcagca ggagcagctt ttccagccaa
     6121 cgattcaaac gagggaaata caaatgaatc caaataatgg tgggaatcat caacaacaga
     6181 atcgtcacca agaccacacc tgctaataaa agtgagcgac ccaccgcagc aataaattcc
     6241 ctgtccgtca gcagctgttg gaaccattgc aaggtaaaat cactaggtaa aagcgatttt
     6301 gaccatttag tggagctggc atacaaaatc gttccaataa tcggcagcaa gtaatacagg
     6361 acaaagcaga acaaaatcgg ttttattaac tttttcacgt ctgcaccacc ttgcctttct
     6421 tttgaataat caacattgcc gttaccagca ggacacctaa agcgactgcc aacgcactgc
     6481 ctaaattagg cttggcgaac acatctcccg acactaaagc cgcaattcga atcgttaaaa
     6541 gatttacatt actgctggtc agggcatacg ctgtttcata ggtccccatg ccattagcaa
     6601 ataagatgac aaaggtgctg gcaatcgatg gtcgcaaaaa aggcaaagcc accttgaacc
     6661 aagcatacgc tgatgaagcg cccagcaatt gtgcggactc cagccattcc tttttcactt
     6721 ccttcatgct gggatacaaa aacaagaccc ctaacggaat ttggaaatag gtatacgtga
     6781 tggtcaatcc ccaccaagaa tacaaattga agtccgctag aaatggcaaa atcattttca
     6841 gcacacctac attccccaac aaaataataa aagagaatgc cagcggaatc ccagcaaaat
     6901 tagctgccaa attggaaaca aaggtgattt tctcctgcaa tttagtcgat agattcatta
     6961 agcataaaca taaaataaac gtcccgatta acccaatcac actggagctg acagcaatca
     7021 gtacgctgtt aacaaaggcc tgtgtgtaat atttgttgct gaaaatatca ctgtaattgg
     7081 ccaatgtcca ctggttagtt aaatcactgt gcaagctgca cagaaccata aaaagcagcg
     7141 gcagcagtaa aaatgcacag aagataaagg aggcaggaac cagaaccaag cgttttttca
     7201 tctctttcat cccctcatca attcttgaat gtcctgcatt ttctctgacg gtgcaattcc
     7261 taataaccag caaaacaatg gagccgccag cagctgtggt acagtttgtt ccttcagctt
     7321 ttggcgttta tttgaaatca aataaaaagg aacttcacga tgtgcagcca agctgccgcc
     7381 atgcagtccg tgcgcatcca tcccgtgatc cgcagtcacc acgacttgat aacctttttg
     7441 cagccattca ggaaggtaaa tagccaataa tctgtccgcc tgattaaccg ctgctgcata
     7501 ttcatgagaa tcggcggtaa atttgtgacc gacatcatca atattcatcg aatgtacgac
     7561 caaaaagtct ggctgatact gattcactaa ataatgggca tccgcaaata aatgcgaatc
     7621 agggtaagtg tcctcactat aaaagatgcc atgctgaatc agtcgctcct cctgatgctg
     7681 gatgcgatca accaaacgat tatagggggc ttgattgtac aactcgctgt accagtggta
     7741 agcggcggct cctgtttttc cgccagcttc cttaactaat tcaaacagcg accactcttt
     7801 ggatcgctga gcatggtaat tactatagat gccattttca taggctggaa cgccggtcat
     7861 cagtacttcg tataacggac gtgaattgct gggcagctca ctttttacct tcaataagct
     7921 tgcttgctgg tgctcaacta aatgattcat gaacccaagc tgggcgacgg ctgtgtcgta
     7981 gcgacagccg tctagtataa ctacaatcag tttgtcaagt ctactttgca taggtcagta
     8041 cctccgcctg ccaaagctct ggaagctcta cagaggtctt gttccaagct tcattgtctt
     8101 caactggcac cgccgcttca taagcatcct ccggcagtaa tttttcagct acttctttag
     8161 gcagctctac cttgcgaata ggtcgcgcat agccctttgc caaattgatt tgcccttcat
     8221 cagataaaat atattcccgt gctagctttg ccgcattggg atgaggggca tttttattga
     8281 ttaaggttgt atacccgcta atgatggagc cgtctgttgg cacagttacc tcaaaacgct
     8341 cggagtcaat ttggtcacga taatttagtc cattgaagtc ccaaacaact gccacatcga
     8401 tttcaccttt ttcaagattt gctacgctgg catcaacggt ggacaaacgg cctttttctg
     8461 ccattttttt gtagtaattc agacctggct gaatatcctt ttcgtcacct ccgttggcaa
     8521 tcgccgcagc caatacagca aattgtgctt ggttggcaat cgtcacatcc cccacgctaa
     8581 cttgatagtc accctcttgc agctccttcc agctagtcgg tgcttgcttc acattctctt
     8641 tatcggtgat aaaggaaatg gttcccgtat agcttaacaa ccaatcccca tcatcatcct
     8701 ttgcccaatc cggaatttcc tcccaatagt tggtcttata aggtaaggtt aactctttgc
     8761 tttcagccaa cggaccgaag ctgataccga catcaccaat atcggcggtt ccattttttc
     8821 cttctgattc aaacttagcc aattcttctg cactggacat atccgtatcc acgtgactta
     8881 agccgtattc tttttcaagg tcgttccaag tgcctaccca atttgcccaa gtatccggca
     8941 ttcccaagct ggcaatctct ccttcttcct tggctttttc tataatcgta tctaaagagt
     9001 cgtctgccgc agccgattgc ttcgttgctt cacctgagcc gcagcctgtc agcaggatac
     9061 ttactgttgc tgcacaaaat aaaccgatag tcatgttacg aattttttca ttcattttca
     9121 ttcattcttc tcccctttcg ataattccat tctaacaaag ctttgtaatg agagaatcag
     9181 tttcttgaaa aggtttcatg aacaatgtaa aggttgtata tggtttgtaa atttcgacga
     9241 attcccgtta accttctaaa gcttttgcgg tatgatagta gaaaaaagga ggctccctat
     9301 gcagtattat gatcagcatc tccacacgta tttttccttt gattcagagg aaaaatttga
     9361 aaactattta gcctatcagc cggagttttt cgtttctacg gatcactttg atttaaacaa
     9421 tccatctacc ggaaaagatg acattccgga ttatccagcc tatataaaca aactggactc
     9481 tttaaaagaa acctattcca ccaaatttct gcgaggtatc gagattggcg ttgttccagg
     9541 acaagaagag cggatcgtgg attacctcgc tcagcatcct tatgatttaa agctggtaag
     9601 tattcatcaa aatggccgct ttgattatat ggacgatgtg gttttaacca aagatcccat
     9661 tcaaactacc aaggaatatt ttgatcagat gttgcaggtc ttgacggatt ttccagaagg
     9721 agatattttg actcactttg attatggatt acgccgcttc accttcacag cggaagaatt
     9781 cgcggagcat tttgaggctc agctgctaac gattttcaaa aaggttgttg aacgagaatt
     9841 ggcgatggag ttaaatgcca agagcttctt gaaatacggc aacgaagacc tttaccgtta
     9901 tgccgttcct ttgtatcaaa gtgtcggcgg aaagctcttt accttaggct cggatgctca
     9961 tgttgccgaa gattatcagc tgggctttaa aaaaatgagt cagctgttgg cagactgtca
    10021 ggtagaaaag ttgctagtca ttcaaggaaa agaacgccgc ctgacgccgc tgccgatttt
    10081 gtgattccta tataatagaa gaaagtggtg aaaaaaatga gcaacctgtg ggactacaat
    10141 caagaggcac ccatccatta tttaattgcg cgacattggg atgcgttgaa aattgaggcg
    10201 gtttgccgat ccttgttagc agccgttcca aaacaacaac tggaaaattt tttggtggct
    10261 gacagcttgc agcgggaaaa agttcaggct tattttgcag cctttaaaga tcagcctttg
    10321 gaatatttgc atgcgcagtt tcatcttttt tatcaagtag ccgccccaga tgactacaac
    10381 gatttacgag gacagctgca attgaccttt caagcggatg aaaccgccta taccgtttta
    10441 ttgggaatgg ctcgccttgg tgatcaggcg aaggtcgagt ggcggatttt cgatatttga
    10501 tggcaggatc aaggcgtttc agttgcaaac cttcctctct ttcaattaga aaaaccgcag
    10561 caggaagaag tttgtgtgct ggtcaccgat gctggtgaaa ttcgtctccg cttatttcct
    10621 gaccaagctc ccctggctgt agccaactgg cgctccctag ctgagcaagg cgtttatgac
    10681 aacacacctt tcggctcgtg tcattaagga atttgttatt caaggtggcg ctttggatgg
    10741 ttctggtgat gaagctgttt ccagctatga gggctttttc gctgatgaag tgcatcgcgg
    10801 actttatcat tttaatggcg ccttggcctt aggggaccac ggcccccaca ccaacggcaa
    10861 ccagtttttt attgtgcaaa acacaaaggc gcaggctgac ctgctaatgt aaagcaagca
    10921 tacttggagc ttggaggact accggagctg gatggtcgtt atactgtttt tggacaggta
    10981 taccaagggt tagaaatcgt agcagcgatc gcagctcagc cgacaaacag tgaagatgta
    11041 ccgattgagc cagtgttcat tcgacaaatt aacttcgaaa aaacatcgta aaaagacact
    11101 cactgacttc agcaaaagct ctattcttga gctggctgca gctagtgagt gtctttcatt
    11161 tatccataag ataaagcttg gattcaataa ataaataaat gttttggtta aagctgtgac
    11221 cagcaaatga ggaaccgtgc gagccagttg atctgccaat gcaaacattt ctttaggtga
    11281 aggcgctggc gtgtctgcca acggatactc cagtcccaat gcttggtaac cgtgcatacc
    11341 atagcagtgg tacggtagta attctaacgg tacgaaccga tcaagatctg ccacaaaaca
    11401 agcaatcgtc agcagctcgg tcaggttcat attgatacct ggaaccacag gaactcgaat
    11461 tcgcacttcg cagttaggat actcaattaa aaagcgacga atattcttca aaatttgttg
    11521 gttatctaag ccggtccatt gtttgtggag cttgctggta aacattttta catccacaaa
    11581 caatgtatcc acatagggag ctaccttttg gatttcatta taagcaccac aaaagctggt
    11641 ttccagtacg gtattgattc caatgtattt cgatttttgc aaaatttctt tagcaaaatc
    11701 cgcctgaacc agtgcctcgc ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa
    11761 aacagcatcc ttctcaatct catccattac ctcttcagcg gtcatggtct ggccaaatcc
    11821 tttttcaatc tgcttgcttt gtgattcggg aatcgaacac caggcacagc gtaatgggca
    11881 gccctttaaa taaacaacgg tccgtaaacc agcaccatca tgtagagaac cctgttctat
    11941 tcttaatact gttccttcca tatagatttt tgaattacac actagtttaa actaaaacac
    12001 acaaggaaaa acttaccatt taatcataaa attagctcat ataaatatac taggatgtaa
    12061 aatagactgg tcaaaatctg gtcaaaaaag aggactaata caggtagtcc tcttttttgt
    12121 tattttaata ttctaaatca ttttttattg gtttattccc ttattcgatg tagactcaaa
    12181 tcactatatc actagatatc aaacggcgtc atacggaatc aaaaacatca aatttgacct
    12241 gttaaatggt cattgaataa cattcaaaat caataaaacg gagtcagaca cggagtcaaa
    12301 aacgctgaga gtaaaaaata tatacagcct tgtaggtaaa gtaccacagg gctgtatatt
    12361 ttttatttca gattacctta aatgattcca tttacttttt atctcatttt ttcctactag
    12421 ttagtatcaa atactctcct attttctcaa taaactattt ttggatacat ggtatctgaa
    12481 acagctaaaa ccttgtcatt acagcaaaaa atcacgttat ccagactgta gacaaagtcc
    12541 attttatgga tgatgtctac agtcttttct tttataatag atttatgaaa acgaatgcaa
    12601 ggagtgatag ctatgatgtc taaaaacaat acgaatggta gaaatcaatt tgcgatgctt
    12661 acgattgatg atctagttcc tcaagatcat ctcgttcgaa agattgatgc cgcgcttgat
    12721 tttgaattta tttatccaat cgtcgaagcc acttactccg atttaggtcg cccaagtatc
    12781 gatccagtga ttctgatcaa attggtgttt attcagtatt tattcggtat ccgttccatg
    12841 cgacaaacca ttaaggaagt cgataccaat gtcgcttacc gctggttttt aggctact
//
LOCUS       DAZKCE010000081        12868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000081 DAZKCE010000000
VERSION     DAZKCE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.082"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1118)
                     /locus_tag="ACG8KQ_002075"
     CDS             complement(<1..1118)
                     /locus_tag="ACG8KQ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9104992.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLE"
     gene            complement(1240..1836)
                     /locus_tag="ACG8KQ_002076"
     CDS             complement(1240..1836)
                     /locus_tag="ACG8KQ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9104993.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2036..4678)
                     /gene="alaS"
                     /locus_tag="ACG8KQ_002077"
     CDS             complement(2036..4678)
                     /gene="alaS"
                     /locus_tag="ACG8KQ_002077"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9104994.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5066..5497
                     /locus_tag="ACG8KQ_002078"
     CDS             5066..5497
                     /locus_tag="ACG8KQ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9104995.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5542..6891)
                     /locus_tag="ACG8KQ_002079"
     CDS             complement(5542..6891)
                     /locus_tag="ACG8KQ_002079"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9104996.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6961..8124)
                     /locus_tag="ACG8KQ_002080"
     CDS             complement(6961..8124)
                     /locus_tag="ACG8KQ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9104997.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8253..8576
                     /locus_tag="ACG8KQ_002081"
     CDS             8253..8576
                     /locus_tag="ACG8KQ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9104998.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8759..10108)
                     /locus_tag="ACG8KQ_002082"
     CDS             complement(8759..10108)
                     /locus_tag="ACG8KQ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9104999.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10177..11130)
                     /locus_tag="ACG8KQ_002083"
     CDS             complement(10177..11130)
                     /locus_tag="ACG8KQ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9105000.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11232..12422)
                     /locus_tag="ACG8KQ_002084"
     CDS             complement(11232..12422)
                     /locus_tag="ACG8KQ_002084"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9105001.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12622..>12868)
                     /locus_tag="ACG8KQ_002085"
     CDS             complement(12622..>12868)
                     /locus_tag="ACG8KQ_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105002.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         3695 a         2712 c         2223 g         4238 t
ORIGIN      
        1 atttccaatt ggtaatctcc gattttttct tctatttgac tacgtaagat tctttctagt
       61 tctgtatcaa caaactggaa tttctcaggg accactacat caaattgcag cgtttttcct
      121 tgatgaatta ctctaaaatc atgaatatta agccctgtac ctgtttcaag aagaatttgc
      181 tttaactgcc gcaagatatt atagtatttt tcgttatcaa caggcattgg atccaagtga
      241 cagacaagtt ccacatccaa agacttttta aagtcttttt ctatcagatc aataatctta
      301 tgagccacat tcaaattcat tgattcatct acttcgatgt ggactgatgc aaagcgttta
      361 ttgggtccat agttatgcac caataaatca tgatagccaa gtatatcatc gcaagcattt
      421 aaatgctctt ccatttcttc aatctctgct ggcgttgggc gacttcctaa tagttcgtat
      481 acaaaatttc gaagcatcgt caatccacta taaaggatat ataatgctaa agcaaaacct
      541 atatatccat caatacgcca tccagttacc cattcgattg ctgctgaaag caatacagcc
      601 agtgtagtaa agacatcatt ataactatct gttgctgtag ctctcaatgt ttcagaatca
      661 atggattgcg caataacctg atacattttg ccttgtgcaa atttcaaaaa gatggaaatg
      721 attaggacaa aaaagacgat gggtgttaaa gagacatttt ctggatggat gattttttca
      781 gcagaagact ctaaaaattg aaatcctaca taagtaatga tgatcgaaac aaataaccca
      841 ctgatatatt cgaagcgttc gtgtccataa ggatgttcct gatctgctgg ctttgcagca
      901 attttaaatc cgatcaaagt caatatggat gaagcagtat ctgatagact gttcactgca
      961 tcagccataa tggagacact tcctgaaaaa agcccgatca ttagcttaga gacaaacaag
     1021 atcagattcg taaccaatcc taagataccc gctagtactc ctttttttgt ccgctgttgc
     1081 ggtatttgtt tctttttttc ttcttgtttc ttagccaagt tttttacccc ttctagtttg
     1141 cttgttgctc aggtaaaaag atggagagga cgattgtttt cctctcctct cttccttttt
     1201 ctatctatcg tgcgtcctat tactattaaa agatggtcat tattctggtt tcaacggtgt
     1261 tgctgcaaat aacgcttttg ctagtaatgg tgttatgatt cccgcaccta ttgcttctgg
     1321 tatcccattg attgctgcaa cccctgctaa ggttttaaat aaaagagcag agtctacacc
     1381 ataggcatct gccgttgcac ctgtataaaa taatcccatg aatcctaaca ctaagactgt
     1441 attgaccaat gaacccagta cccctgcaaa ggctgagctg atcgttattg atttattttt
     1501 ttgataaatc cagtggaaaa ccaagcctgc tactaagcca acaagtactc tgggaactac
     1561 cgagataatc ggattcgtga acactaatgt atcaaatggc gttgttggca tggcataggc
     1621 acgaacaatt gtagcgattc cccaaaacaa gccaatcacc atgccatctt ttgggcctaa
     1681 tactactgca gcaataatta ctgtgatatg gataattgtt aaactaatga atcctaatgg
     1741 aataaacccc agccaaggaa ccatcgcttg tacgatgatg attgctaaca agatcgcacg
     1801 taagactagt cgaaaggtct tatttctact attcattttt tcaccccttt ttatttatac
     1861 taatcgtaaa aatttctgtc actttcgtca actaaagacc atccgaatcc gaccatttga
     1921 ttagatgcgc catccaattg aaaaaagtat gagggcaaga cttgtttgtc ttcgccccca
     1981 taatcatgtt tcgttcaatg tttgaacttt tgtttttctg tcaaacgtaa acgccctatt
     2041 gttgcgcaac ccattctttc gcagctttaa gtgcatctgg aataccagca ggatttttcc
     2101 cgccagcttg tgccatgtct ggacgtccac caccgccacc gcctactttc ggtgcgattg
     2161 ctttaatcaa gtcgccagct tttagtccag cttcatttga ttctttcgtc attgccgcta
     2221 gcagactgac ttttccttct tgttcgtttg ctaaaacaag aatatctgaa atattttttt
     2281 gtttccattg gtcagctaat tgacgcagct gattcatatc tttcactttt acttgtgctg
     2341 caatgattgt ttttccattg atcgtttctg gatctttgaa tacatcacca gcttgttgat
     2401 tcgctaactt gctagctaat tgttcatttt ccttttgtag ctctcttact tgctgttgca
     2461 attgttccgc ttttgaaaca acttctttca attgaggtga tttaacaatt gttgctactt
     2521 gttttagttg tttttcttct tcttcaagta gttcgtacgc ttctttactt gttacagcct
     2581 cgattcgtcg cacgcctgca ccaattcctg attcagaaac gattttgaag attccgatgt
     2641 tttctgtatt tgctacatgt gtgcctccgc acagttcgat tgaccagccg ccaatattta
     2701 ctactcgaac gtcatgtccg tatttttcac caaacaaagc cattgcaccc atgtttttgg
     2761 ctgtatcgat atctgtttca attgtttcaa ctgggatagc ttcccagatt ttttcgttga
     2821 cgattctttc catttcagct aattcatctg aagtgatttg accaaagtgg gtaaagtcga
     2881 atcgtaaatg tccaggagcg actaaagaac cagcttgatt tgcatgctcg ccaagtactt
     2941 ctttcaatgc cttgtgcagt agatgggttg ctgtatggtt tttgatgatc cgattgcgca
     3001 tcgattgatc gatcaataga tgataggttt gtccttcact cattggtgct tttacttcta
     3061 cggtgtgcat aaattgacca ttaggcgctt tttgaacatc tgtaacaact gctacgattt
     3121 cgcctacttc attcatgatc catccttgat ccgctacttg cccacccatt tctgcataga
     3181 aaggtgtttg atcaaagatc atttgagctt caccttctgc gacttcattc aaaagagact
     3241 cacctttaac gatgaccaat aattgactat ctgattcttg ttctgtgtat ccaatgaatt
     3301 tactttctac tttgatatct gtcaataaag cagattgtac ccccattgat ttttcagtac
     3361 tgcgggcaga gcgagcacgt tcgcgttgtg catccatttc tttttcaaaa ccagcatgat
     3421 caactttcag tccttcatct tctgccacct cttccgttaa ttctaccggg aagccatatg
     3481 tgtcgtatag tttgaagatg tcttttcctg ctagagtcgt cccgttttcg ttttttactt
     3541 cttggatcac ttgatttaag atttccaaac cttcattgat tgtttcattg aaacgttctt
     3601 cttctgtacg gataactttt tcaataaatt ctttttgttg aagtacttca ggataataac
     3661 ttaccatgat ttccccaaca acagggacta aacgatataa gaatgcttgt tcaataccta
     3721 attttttccc atgcatgaca gcacgacgaa gcaaacggcg taagacatac cctctccctt
     3781 cattcgatgg caatgcaccg tcgccaatag caaatgaaag ggcgcgaata tgatctgcga
     3841 ttactttgta agaaatatct gttactgggt tttcaccata ttttacttgt ttgcttagct
     3901 tttctacggc gtggataata ggcataaaca agtctgtctc aaagttcgtt gacgcatctt
     3961 gaacgattga gaccatgcgt tccaatccca tccccgtatc aatgttttta tgaggaagtg
     4021 gttcaaatgt gttatcaggc ttatggttga attctgaaaa tactaagttc cagatttcta
     4081 aataacgttc attttctcct cctggatagt tttcaggatc gtcttctgct aaatcgttat
     4141 aagcttctcc acggtcatag aaaatttccg tatccggacc acatgggcct gcaccgatat
     4201 cccagaagtt gtcttctaca tcaataatgt gatctagtgg caggccaact tcttcatgcc
     4261 agatgcgttt tgcttctgta tcttttggat aaactgttac ataaagtttt tctggatcga
     4321 atgccatcca ttttggatca gtcaaaaatt cccatgccca gtggatggct tcatttttga
     4381 aatagtcacc aatcgaaaaa ttacccagca tctcaaacat cgtatggtgc cgtgctgttt
     4441 taccgacatt ttcgatatca tttgttcgaa tagatttttg ggcatttgtg attcttggat
     4501 tttctggcac aactgaacca tcaaaatatt ttttcaatgt tgcaacacca gagttgatcc
     4561 acaataaggt tggatcgttc acggggatca aagacgcact aggttcaatc gtatgtcctt
     4621 tagattggaa gaaatcaaga aacatttgtc gaacttgtgc actacttaat tgtttcatgt
     4681 cattacctgc tttctataaa ttaaatacaa aaaaacacca tcacagccaa ggacgaaaac
     4741 tcgcggtacc accttgattg caatgatgtg taatcattac ctcttaaact cgataacgct
     4801 gagctgcgtc ggtatttcta ccaaaataga aaaagggagc aatttgatta gtgactattc
     4861 ttttttcagc caagaaagaa ttttctgtga gtcttaaaaa acaaatcata tccttttcgt
     4921 acttaattag tatacgcatt ctattgatca ctgtcaatat ctcaattttt attttcattt
     4981 ccttttggtc atactccacg atttttggta cgataagaat aaaggaatca atacatctct
     5041 gctattcatg acaaggagga atatgatgaa aaaaatagtt ggagcggatg aatggatacg
     5101 cgcagctagc tgctttttga gatatcaagt ttttgtatta gaacaaggga ttcctgcaga
     5161 actagaattc gacggaaaag atatcccgca acaaacctat tatctatttc ttgatcaaaa
     5221 aagaccagta gctactttgc gttatgaaaa gaaaacacct acttgcttca agccggaacg
     5281 attttgcgta gcaaaagaat accgaggaaa agggttaggc ggtcagttgc ttgaagctgc
     5341 tgaaaaacgg gcggtttcag aaggttgtca gactagttat gtagtagctg aaatgacggc
     5401 tgtagatttt tatgaaaaac aagggtatca tacttgcaca gaacctttta ttgaagatga
     5461 agtactttgt atcggaatga aaaaaagtct agcttaaaaa caagagattg tgacacgcct
     5521 tttgtcacaa cctcttgttt tttatttatg tgaatttttc ttatttttac gagcagtacg
     5581 gctttgctgt ctgcgttcga ttttgcgttc ttttttattt ttttctgcaa ttgcccaatc
     5641 gatttttttc ttataaccag gcttgatctt tttctttttc ttcttcacaa gaccaatcag
     5701 cgtgggatct aatacatctt ttgatttttc tcgtttggca cgtctattac gatcgtatgt
     5761 tttgacgatt tccccgtttt tgatttcttt tggttcaaat gagacgccca atttttctat
     5821 cgcatcaatg gcttgctcat catttggcga atataacgta atggccgttc cttttaagcc
     5881 attacgtcca gtacgtccga cacgatggat aaagaagtct agatcttctg gaatttctgc
     5941 attaataaca tgagagacac cttcgatatc gatccctcta gcagctaaat cagtcgctac
     6001 aacatattga tactccagat tctgtacttg tcgcatcact cgttttctct ctcttggggt
     6061 gatgtctcca tgaatctttg ctactttcaa gccttggttt ttcaagtaat cggcaatttc
     6121 atccacatgc tgttttgtat tggcaaatac gattgctaaa taaggatgac caattgtcag
     6181 cagttgataa atcatttgat tctcatcttt accttttgtt gaaatcagcc aattttcaat
     6241 gtcttcagaa ataactgctt ttggtttgat ttcttcaatg attggatttt ccatgtattt
     6301 tcgtaagaat ggacgaagtt tttctggaat agtggcagaa aaaactaaaa actgcagctt
     6361 ttctggtaat cgacttgcaa tttggtctac ttcatttaaa aaacccatat ccaatgtcat
     6421 atccgcttca tcgacaacaa atgcaaatgc ggtgtgaatc ttcagcgctt gttcattcat
     6481 catgtctaat attctaccag gcgtgcctac tacaacctgt ggctgttggt gatgcagacg
     6541 atttaattga cgctgtttgt ctgtaccgcc gacaaaattc gagacacgga tctccggtgt
     6601 agaaaattta gcgatttgtg tagctgcttc atagatctgt gcagctagtt ccctacttgg
     6661 agctgtaata acaacttgta cttcctcttt ttctgcattg attttttcaa acaaaggcaa
     6721 caaaaacgta tgtgtttttc ctgtaccagt ttgagattgc ccaatgatat tttttccttt
     6781 tgcaattaaa ggaatcaaac gttcctggac ctctgtgggc tgtttgaagc cctttgcggt
     6841 taacgcttca ttgataaacg gtttgaagcg gaatatatca aaattattca ttcgtgcacc
     6901 tcttctcttt aaattatcac gactttggca ttataacaca gacaaagcgt ttttgctcag
     6961 ctaatttcga gaatcatcat cctttgtttg gccaatcgtg ctaacagtat atgtgtgact
     7021 agctttttca taaaacagaa tccgtttttt tctcaaaaat gatagcaaga tatttaaaat
     7081 aaaaatacct tgtagtaaca agacaccgag agaaatgaat actgcactca tctgcaacac
     7141 cgtattcgtt gtcgaattca tgatttgctg aagcaactgc cccaaccagc ttaaaccata
     7201 aaaaagaaag tacagatacc cgtagcggat catcaatgct tttaaagaaa ttcgttcgcg
     7261 gtcttcttcc accaaacgga tacgcacaat ttttttgcca agtgtccatc cattggttat
     7321 aaacggtaca atcataaagt atacccctac ttctaataaa agccaccaga tatgagaagt
     7381 aactgcccct gaatcgaacg caattttaaa gaaaatcgcc gctaccaaat taataagaga
     7441 taaaaatgtc cagtcgatca aataagcgac caagcgtcga gtcaatgaga cagtctttcc
     7501 tttttcataa gaaagttcat ccattcgtct tcttgtagga agcataaatg taaataacgg
     7561 cgtaacgaca aaaccaatga ttccacccaa tgtatttgtc actaaatcat tcacatcaaa
     7621 taagcggtat ggtcttgggt agataaaata aagtccactt agctgcgtca attcaaaaaa
     7681 tagagaaagg cagaaactag caatcatcgt tttcataaaa gaaagccgga aataatatct
     7741 taaataaatg ccaaacggta tgagtaataa aacattaaaa gctggttcca aaaatacact
     7801 ctgcttcatt gccggcaggt aggtactagg ttcagacaag accaataccg tgttgtctct
     7861 gaaatgttgc catgaagcaa ataaatgcag ctccatcttt ggccctgtca tttgggcaac
     7921 cgtctccttt gccggaagtg gtaagatcac caaaaaatac gcacacaata aataaaaaac
     7981 gaaagaatac aaaataactg cccttgttac tagaaatgtt ccatactttc gatattcata
     8041 aataaaaaaa acagatgaga tcgctaaggc taaaaatgga aaaacaatca gcgcaaaacg
     8101 aatcggttcg atataacttg ccatatgact cctccttgat tctttgtatc tctcttatct
     8161 tagcagtcct ttgctgtttc aagcaagtaa cgaggctatt atttctcatt tctttcaaag
     8221 taaagtaaga taaacacaaa gggggtgtcg agatgctgaa tatgtacttt gtttttggcg
     8281 tacctatttt tttactgttc ctttatgcta cgatcgctta tgtaagaaaa agaactacaa
     8341 ttcattattt aggatttatt ctcctgatta tttcgggttt catgctagtc ttcaatcttc
     8401 agacttggca gcaagcactt cttgaaatgg ataaaatgac tccacatgct ttgtctaaag
     8461 tgcttggcta ccctgtctat ctgatttggc ttcccatttt catttctggt tgcttggttt
     8521 tattaaatat ttatcgcggc gtacgccgaa tagttcagtt acgcaaatca aagtaaaaga
     8581 cagaaatcgg ttgagcccga tttctgtctt ttactttgct atttcatcgt agacaaaacg
     8641 acatttacgt gatcaaaatt aacgagtact tttgacattt cttccacgta ctcctcattt
     8701 aaataaaata ttttgtttgg cagagaagtg cagttctaat aaaattcgaa tgttttagct
     8761 atctacgttt taaatagaga taaatcggga taccagcaag tgtcaatact atccccatcg
     8821 aagctaaaaa ggtctgagtg ataagcgtgc ttgatacgat aaaagtccct ccaatgattg
     8881 ctactagtgg aataaacgga tacattggaa ctttgtatgg tcgaaacaaa tcaggttctt
     8941 ttttacgaag aagaatgaca ccaacaaaga ccatcgtata aaaaatccaa atgacaaaga
     9001 tcagcatatc cgttagtgta tcaaaaccac cgatcatcat catgccaata gcgatgacta
     9061 gctctaagat acctgcggct acaggaactt tagttttatc atgaagttta gcaaagagct
     9121 tgctaaaagg caggttgttt tcttttgcca taacataagg gagacgcatt ccagtaaggg
     9181 tatagccatt gatggttcca taaacagaaa tcaagatacc aactgtgacc agtcttccac
     9241 cgaagccgcc aaaaatcatt tttgcaacgt cacttgccgc attgctgttt cctgcgactc
     9301 cgtcgataga tgcggttctt aagaacaccg catttactaa taaataaacg atcatgattc
     9361 cgatgattcc taaagaaatt gcttttggca aatctttcgc cggctttttc aattctcccg
     9421 aaatatttcc tacgtgtatc caaccgtcgt atgcgaacat cgttgctagc aagcccgctc
     9481 ccaaggctga gaaaaatgaa agattttctc ctgcttggat cggaaacagt tggaagtcta
     9541 ctccttcttg tcggaacaaa ccgaaaatga cgatcacaaa caaaggaata agcttgcaga
     9601 caagcgtaat cgactgaaaa gcccctccag ctttagaacc taaaaaattg atcaacagga
     9661 tcgatacagc agctgtaaca gctacgggaa caatcattga ctgggaaaga ccaaatagat
     9721 tgacgaactg tgtaccgaaa atgatcgaca aggctgctac atttgctgga aaataaatca
     9781 cgacttgcgc ccagcccaat aaaaaagctg ctgtatttcc gtaaatccgt tcgatatatt
     9841 taatcatccc tcctgtttct ggaatagcag ctgccagttc agctcctgtt agtcctgcac
     9901 agacagaaat cataccaccg agaaaccaag aaaacatatg caaactggcg gatcctgtaa
     9961 cttctgctac gcttgcggct ttaaaaaata cccctgcacc gatcactgtg cccataacag
    10021 tcgataaagc agtaaaaaat cccatcgtac gttttaattc attttctatt tttacttctt
    10081 ttactggcgt ggcttgatat tcttccatcc cttgtcccct ttcataccat agccaatccc
    10141 aaaagtataa aaaacttttt ggatgtggtt cttgttttat accgtctcta ccaatttttc
    10201 tttcagtttt tccaacatat cctttgtcat cacatctaag tcgtatttcg gcgaaaagcc
    10261 ccattcctct tgcgcacaag aaacatctag tgaatttggc caagattctg ctattgactg
    10321 acgtacagga tccacatcat aatccatttc aaaatctggt atgatttttt gaatagatgc
    10381 tttgatctct tccgggtcaa aagacatcgc cgatatattg aaggcatttc gatgaaccaa
    10441 tcgatcagga tctgcattca tgagttttat aatggcatcg atcgcatctg gcatatacat
    10501 catatccatt gatgtacctt gatcaatgaa actagtgtac tttccttctt tgattgctga
    10561 ataataaata tcgactgcgt agtctgtcgt accgcctcct ggcaaggttg cataagaaat
    10621 caatccaggg aaacggacac cacgagtatc tacgccatat ttcacataat aataatcaca
    10681 tagtagttct ccagctacct tcgttacccc atacatggtt gtaggacgct gaagagtatc
    10741 ttgtggggta tgatcttttg gagtatttgg accaaacgcg ccgattgaac ttggcgtaaa
    10801 aaatttcagc tggaattctc tagcgacttc caatgcattg accagcccag tcacgttcaa
    10861 attccaggct aattgtggtt ttgcttctgc aacagctgac aaaagagcag ccaaatgaat
    10921 caatgtgtcc acttgatatt gttctgctat gctgcgcatt ttttcgtaat ccattacatc
    10981 taggatctca aagatatcgt ttttctcaat gacttcaggc atgcgtatat ctgtcgcaat
    11041 gacattttca ctgcctagtt cttgacgtag ccgtgtgatc aactctgaac caatttgtcc
    11101 taaacatcct gtcaccaata cttttttcat gatgatcctc cctgatcttt tttactacta
    11161 tcttcagatg attaacaata tatgcgtgaa catgttctct aattttaaat agacgaaaaa
    11221 actaaattta gttaaataat ttccatttct tttcctattt tttgataaac tgctactgct
    11281 tcatcaagca tttccctcgt atgttccgca gtcggcatat ttctgatcct gcctgtccca
    11341 agaggcacag ttggataaac gattggttta gcataaatcc catgttcgat cagcttctta
    11401 gagaaagtct gagtcacttt ttcgtctccc aaaatgactg gagtaatagg tgtttcagac
    11461 ataccgatgt tatagcctac ttttttcaat tcttctttaa aataattagc attttcccaa
    11521 actttttcga caagttccgg atgttcttgc atcaaagtaa tcgaagccaa tgcagctgca
    11581 gctgcacctg gagttagcga agtagaaaat aggaatggcc gacttctagc tttcaaccag
    11641 tcgattagtg ttttcgatcc agctacataa cccccaacaa cacctatagc tttcgataac
    11701 gtgcccattt gcatatcgat tttgtcgctt aatccaaaat gcttgacggt tcctgcacct
    11761 tttccagtta cgcctgagcc gtgcgcatca tcgacgtagg tgatcagtcc atatttttca
    11821 acaatcggaa taatttccgg caatcgtgca atgtctccat ccatggagaa aacaccatct
    11881 gtgatataca tgattttttt atatttctta ctttctatcg cttcctttgc ttttttctca
    11941 aggtctttca tatcttgatg tttaattcgg atgatttttg ctccggataa gcggcaaccg
    12001 tcaataatag atgcatggtt taattcatcc gataagatag catcttcttt tgtcattact
    12061 gcagagatag cgcccatatt gcagttgaat ccagattgaa aggcgattgc tgcttctgtc
    12121 cctttgaatt ctgcgatttt ttcttctaat tgctgatgga ttttcagcga accattgatc
    12181 gttcggactg ctcctgctcc tacaccataa gtttcggttg cttcaataca tgcttttttc
    12241 aattcttcgc gattggcaaa tccaagataa ttgttggatg ctagattgat catctttttt
    12301 ccgtctacga tgatacaagc accattttca ctttctagta tgtcgatcgt attgtataac
    12361 cccttttctt tcagttgatc caattctttc tctaaataca ttgttagttc tttggaagac
    12421 attgtctcca cctccatatt catctgtctt gcagaacata ccaaacatat atcgtgtcat
    12481 tcatctacaa agcaagtatg ctatattttt gataacgctt acactgatta tgagaattat
    12541 ttaaccacat tttttattta cagtatcttt ttactttcaa atgacttata tagtatgaac
    12601 aattaccttt tagataatga tctattttac tctacctggg cagataaata caacacaacc
    12661 attgatagaa gccctttcgt taaaatcaat tgtattctga atatttaatt tatttattgc
    12721 atagaccaat aaaaatacat aaaatttcca tcttcgttta tcagcaattt ttaattttac
    12781 tctctgaata tttcttaaaa gttttacttg ttgaattcga aaaaaggact gtgacaaaag
    12841 tcttgtcaca gtcctattct tccgaata
//
LOCUS       DAZKCE010000082        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000082 DAZKCE010000000
VERSION     DAZKCE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.083"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8KQ_002086"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8KQ_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9105003.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8KQ_002087"
     CDS             complement(323..892)
                     /locus_tag="ACG8KQ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9105004.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8KQ_002088"
     CDS             1078..1980
                     /locus_tag="ACG8KQ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9105005.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8KQ_002089"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8KQ_002089"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9105006.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8KQ_002090"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8KQ_002090"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9105007.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8KQ_002091"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8KQ_002091"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9105008.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8KQ_002092"
     CDS             8554..8739
                     /locus_tag="ACG8KQ_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9105009.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8KQ_002093"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8KQ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9105010.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8KQ_002094"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8KQ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9105011.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8KQ_002095"
     CDS             11896..12705
                     /locus_tag="ACG8KQ_002095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9105012.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKCE010000083        12704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000083 DAZKCE010000000
VERSION     DAZKCE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.084"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..765
                     /locus_tag="ACG8KQ_002096"
     CDS             28..765
                     /locus_tag="ACG8KQ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9105013.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            779..1594
                     /locus_tag="ACG8KQ_002097"
     CDS             779..1594
                     /locus_tag="ACG8KQ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9105014.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1607..2254
                     /locus_tag="ACG8KQ_002098"
     CDS             1607..2254
                     /locus_tag="ACG8KQ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9105015.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2270..2926
                     /locus_tag="ACG8KQ_002099"
     CDS             2270..2926
                     /locus_tag="ACG8KQ_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9105016.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3088..3909
                     /gene="racE"
                     /locus_tag="ACG8KQ_002100"
     CDS             3088..3909
                     /gene="racE"
                     /locus_tag="ACG8KQ_002100"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9105017.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3912..5267
                     /gene="rph"
                     /locus_tag="ACG8KQ_002101"
     CDS             3912..5267
                     /gene="rph"
                     /locus_tag="ACG8KQ_002101"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9105018.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5268..5786
                     /locus_tag="ACG8KQ_002102"
     CDS             5268..5786
                     /locus_tag="ACG8KQ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9105019.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5893..6393
                     /gene="cbpB"
                     /locus_tag="ACG8KQ_002103"
     CDS             5893..6393
                     /gene="cbpB"
                     /locus_tag="ACG8KQ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9105020.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6673..7593)
                     /locus_tag="ACG8KQ_002104"
     CDS             complement(6673..7593)
                     /locus_tag="ACG8KQ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9105021.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7719..8771
                     /locus_tag="ACG8KQ_002105"
     CDS             7719..8771
                     /locus_tag="ACG8KQ_002105"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9105022.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8881..9636)
                     /gene="fabI"
                     /locus_tag="ACG8KQ_002106"
     CDS             complement(8881..9636)
                     /gene="fabI"
                     /locus_tag="ACG8KQ_002106"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9105023.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9856..10296
                     /gene="fabZ"
                     /locus_tag="ACG8KQ_002107"
     CDS             9856..10296
                     /gene="fabZ"
                     /locus_tag="ACG8KQ_002107"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9105024.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10411..11172)
                     /locus_tag="ACG8KQ_002108"
     CDS             complement(10411..11172)
                     /locus_tag="ACG8KQ_002108"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9105025.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11361..12455
                     /locus_tag="ACG8KQ_002109"
     CDS             11361..12455
                     /locus_tag="ACG8KQ_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9105026.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12541..>12704)
                     /locus_tag="ACG8KQ_002110"
     CDS             complement(12541..>12704)
                     /locus_tag="ACG8KQ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9105027.1"
                     /translation="GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYV
                     QEAFYSSLI"
BASE COUNT         4090 a         2199 c         2670 g         3745 t
ORIGIN      
        1 actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca cgtggataaa
       61 tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa aggagaagtt
      121 gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg tatcaatgga
      181 ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca tgataaaaag
      241 acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt caatttgtac
      301 ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt gaagcaagac
      361 caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat gttagataag
      421 aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc aatcgccaga
      481 ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc tttggatccg
      541 gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg tatgtcaatg
      601 attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt tatcttcatg
      661 gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga aaatccaaat
      721 gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga ggaaaaagat
      781 gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc ttgcactagc
      841 cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca aatctaccaa
      901 tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg atattgagag
      961 cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa agatccttgg
     1021 tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc cattattgaa
     1081 aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac gaaaaaaaca
     1141 ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa agaaaggaag
     1201 ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag tcaaaggttc
     1261 tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag aattagaaaa
     1321 ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga ctacggataa
     1381 tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg gtggaacgtt
     1441 tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct tgaaagctgt
     1501 taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct atcaaaaatg
     1561 gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga gcgaattgat
     1621 acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt cgagtgtctt
     1681 agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat tatcaaagaa
     1741 taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca ttccattatt
     1801 aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc tagatggatt
     1861 ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg aaacagtacg
     1921 ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt ctggttttac
     1981 ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg ttatcccgcc
     2041 attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa tcgtagctgg
     2101 tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata ccttcgatac
     2161 gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt acttaatggt
     2221 ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca tggattttag
     2281 cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg ttactgttga
     2341 agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag gtgtgattcg
     2401 ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata ttatacggaa
     2461 tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa tcggcattca
     2521 gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag ctatgttgtc
     2581 ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag caggattatc
     2641 aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag catttaaatc
     2701 aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt cattagcagt
     2761 aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac agaacaccaa
     2821 ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat gttacgcgct
     2881 ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga tgattctttt
     2941 agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga aaaaaagaga
     3001 tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag tacacaatag
     3061 ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg ccctatcgga
     3121 tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa acaattaccg
     3181 aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc tagacccgcg
     3241 gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca aggaatcaag
     3301 atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat caaagctgct
     3361 ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt aaaaaaaaca
     3421 caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca agcttatgaa
     3481 aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg tcccaaattt
     3541 gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat tgtggcagaa
     3601 acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg cacccattat
     3661 ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact gatcgattct
     3721 ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt gagcaattca
     3781 ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa acttttcgag
     3841 gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat cgaattggga
     3901 ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa ctggagcttc
     3961 aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt gatacaacag
     4021 ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa acaggaacag
     4081 gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga aatcgtagag
     4141 aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt attggtcgtt
     4201 ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc gtagactgcg
     4261 atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca tttgtggcat
     4321 tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat ccaataaaag
     4381 aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc acagatttgg
     4441 attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca gaatctggaa
     4501 aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa cagttgaatg
     4561 aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa aaacatgctt
     4621 tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca atcatcattg
     4681 cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa gcgggatatc
     4741 atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact ggcagcacat
     4801 ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat cagccagtct
     4861 tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga atctattctg
     4921 cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta ttatatgaat
     4981 tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta gtattcgcgg
     5041 cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta gcccgtatac
     5101 catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt aagaaacaaa
     5161 cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct caagcaatga
     5221 aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg cggtatttag
     5281 tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat cgttatcgtt
     5341 ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct gacccattat
     5401 gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc gaacagaaaa
     5461 aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta tcgaatgtcc
     5521 gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat atggtgtttt
     5581 ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt ttgaatcctg
     5641 gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt atcgaaagtt
     5701 tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt gaagagttgg
     5761 ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata aaaataagct
     5821 ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg aaatagaaat
     5881 ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa tcaggaaacg
     5941 tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt gtcccatggt
     6001 ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa agatgatcac
     6061 tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt gcaaacaatc
     6121 agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga tgttccgaca
     6181 attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc gcctttttta
     6241 ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga gctgcttaaa
     6301 gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc gaaaatcgaa
     6361 gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa gataatcaaa
     6421 aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga ggctgggaca
     6481 aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc aaatttttgt
     6541 gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac gtgtttagag
     6601 cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga ttcgttgcat
     6661 cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga aaccagaagc
     6721 tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt gttttccgtt
     6781 caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca ctaatccaaa
     6841 gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac gatttactcg
     6901 atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa tatctaaaag
     6961 tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat tcgggatgaa
     7021 atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag atacgacggt
     7081 tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct gttgttccat
     7141 gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa gaccgattgc
     7201 cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga tagcagataa
     7261 aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta aaagactatg
     7321 taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat taaaagactt
     7381 atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca aaaaaatcag
     7441 atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc cgtcccaaaa
     7501 ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc cagaagagtc
     7561 tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa aatttgattc
     7621 tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac tataatgaag
     7681 gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt ggagaaaata
     7741 acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct tatatcaaaa
     7801 ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga gaattgacag
     7861 aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt cagcaatgtt
     7921 ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt cgacctgaac
     7981 aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg gtccctgaaa
     8041 agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt gtgccgccct
     8101 atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt gaaaacattg
     8161 gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta ggagcgtatt
     8221 tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt gctgctccta
     8281 aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt ccaggaacag
     8341 aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca catacgaaaa
     8401 tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa ttcatcactc
     8461 cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag ttagctgaag
     8521 aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta gatcaatttg
     8581 cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg atttatcaca
     8641 ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca aaacggttgt
     8701 atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc tggattgaag
     8761 tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa atcaggaaga
     8821 gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg caacaaaagt
     8881 ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc cgctggaaag
     8941 tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac cttgctgatc
     9001 aggcgtacga gattcagata actggatcag tttttgatag tctttaacac ctgtgacagc
     9061 taatgttttg attgccccag ctgagattcc attgacccga attcctttag gcgagaactc
     9121 agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca tcatgttata
     9181 attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc tgtttggtgc
     9241 aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga tatcttgagc
     9301 taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt ctttgttggc
     9361 gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga tggcattcat
     9421 tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg cttcttcgcc
     9481 gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga taatttctgc
     9541 cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt tagcgacgcc
     9601 cataatgacg attttttttc cttctagaaa tgacataatg actctccttt gtttctgata
     9661 aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct tgatttctta
     9721 attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa aaaagattgc
     9781 taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca attgcctgaa
     9841 agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga attgattcct
     9901 aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg aaaaaagatc
     9961 gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt tcctgacaat
    10021 ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc gatacttatc
    10081 ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa taaggccaaa
    10141 ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat caccaaaatg
    10201 aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa agtctgtgag
    10261 tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat atagcaaaac
    10321 aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta gcatacgttg
    10381 tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt gcagtatatt
    10441 gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt cgatatttgc
    10501 aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat aatggccatc
    10561 tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat agtcgtcttt
    10621 taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc tgccgttact
    10681 gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa ggtggttgtt
    10741 gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat cgcctttagg
    10801 atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc cgttgtagta
    10861 accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt cgggaaataa
    10921 tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat tttcggcgac
    10981 atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg cagtcgtgct
    11041 ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag tcgtgttgtt
    11101 tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta aaataacttt
    11161 atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa tgataacaaa
    11221 atagagggaa aatacctact taaattattg gaatttatca tttattttaa aagaaaaaga
    11281 aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa aaacgaaaag
    11341 atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca gaacggtcaa
    11401 catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat aaacgggttt
    11461 ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa tttaaagaag
    11521 tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat ccagcgttat
    11581 ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg ggtgcctacg
    11641 gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg cgcaatcctt
    11701 ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac aacatcatga
    11761 gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct catcgatact
    11821 ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa tataatttga
    11881 atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct acgcaagatg
    11941 gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg aagcatttaa
    12001 tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt gaagaagaat
    12061 tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta gatacggaag
    12121 aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt tatcgagggg
    12181 atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa gataaagatt
    12241 tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac aatgaagggt
    12301 acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac gacggacgtg
    12361 tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt ttgaaaccat
    12421 ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc agaaataatc
    12481 cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg ttatgatttg
    12541 tcaaattaag ctagaataaa atgcttcttg tacatagctc aaaaatattt cgattggtgt
    12601 tctgtaattc aatgattttc ttggaatatg attacgttga ttgctgacac tggaaataaa
    12661 tgtctgattc acttttctaa aatccattga tttcggcagt ccat
//
LOCUS       DAZKCE010000084        12492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000084 DAZKCE010000000
VERSION     DAZKCE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.085"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8KQ_002111"
     CDS             <1..375
                     /locus_tag="ACG8KQ_002111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105028.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8KQ_002112"
     CDS             complement(324..893)
                     /locus_tag="ACG8KQ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9105029.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8KQ_002113"
     CDS             complement(967..2256)
                     /locus_tag="ACG8KQ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105030.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8KQ_002114"
     CDS             2514..3458
                     /locus_tag="ACG8KQ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9105031.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8KQ_002115"
     CDS             3472..4545
                     /locus_tag="ACG8KQ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9105032.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8KQ_002116"
     CDS             4535..6706
                     /locus_tag="ACG8KQ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFP9105033.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8KQ_002117"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8KQ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9105034.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8KQ_002118"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8KQ_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9105035.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8KQ_002119"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8KQ_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9105036.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8KQ_002120"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8KQ_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9105037.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8KQ_002121"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8KQ_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aa
//
LOCUS       DAZKCE010000085        12343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000085 DAZKCE010000000
VERSION     DAZKCE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.086"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      8..174
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            322..879
                     /locus_tag="ACG8KQ_002122"
                     /pseudo
     CDS             322..879
                     /locus_tag="ACG8KQ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            937..2976
                     /locus_tag="ACG8KQ_002123"
     CDS             937..2976
                     /locus_tag="ACG8KQ_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9105038.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3085..4236)
                     /locus_tag="ACG8KQ_002124"
     CDS             complement(3085..4236)
                     /locus_tag="ACG8KQ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9105039.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4390..4920)
                     /locus_tag="ACG8KQ_002125"
     CDS             complement(4390..4920)
                     /locus_tag="ACG8KQ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9105040.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5019..5369)
                     /locus_tag="ACG8KQ_002126"
     CDS             complement(5019..5369)
                     /locus_tag="ACG8KQ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9105041.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5526..7055)
                     /gene="ahpF"
                     /locus_tag="ACG8KQ_002127"
     CDS             complement(5526..7055)
                     /gene="ahpF"
                     /locus_tag="ACG8KQ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9105042.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7065..7628)
                     /gene="ahpC"
                     /locus_tag="ACG8KQ_002128"
     CDS             complement(7065..7628)
                     /gene="ahpC"
                     /locus_tag="ACG8KQ_002128"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9105043.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7772..8434)
                     /locus_tag="ACG8KQ_002129"
     CDS             complement(7772..8434)
                     /locus_tag="ACG8KQ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9105044.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8618..10264
                     /locus_tag="ACG8KQ_002130"
     CDS             8618..10264
                     /locus_tag="ACG8KQ_002130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9105045.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10255..11337
                     /gene="uxuA"
                     /locus_tag="ACG8KQ_002131"
     CDS             10255..11337
                     /gene="uxuA"
                     /locus_tag="ACG8KQ_002131"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9105046.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11445..>12343)
                     /locus_tag="ACG8KQ_002132"
     CDS             complement(11445..>12343)
                     /locus_tag="ACG8KQ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP9105047.1"
                     /translation="FLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLSVDILRPNLP
                     ICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLF
                     IPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDF
                     REQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIE
                     QIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISE
                     YIFELKEEALMDQSKKENQVTS"
BASE COUNT         4116 a         2280 c         2322 g         3625 t
ORIGIN      
        1 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
       61 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      121 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      181 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      241 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      301 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      361 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      421 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      481 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      541 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      601 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      661 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      721 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      781 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
      841 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
      901 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
      961 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1021 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1081 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1141 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1201 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1261 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1321 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1381 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1441 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1501 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1561 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1621 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1681 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1741 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1801 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     1861 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     1921 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     1981 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2041 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2101 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2161 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2221 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2281 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2341 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2401 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2461 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2521 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2581 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2641 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2701 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2761 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     2821 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     2881 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     2941 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3001 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3061 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3121 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3181 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3241 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3301 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3361 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3421 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3481 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3541 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3601 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3661 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3721 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3781 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     3841 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     3901 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     3961 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4021 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4081 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4141 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4201 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4261 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4321 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4381 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4441 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4501 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4561 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4621 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4681 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4741 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4801 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     4861 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     4921 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     4981 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5041 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5101 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5161 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5221 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5281 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5341 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5401 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     5461 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     5521 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     5581 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     5641 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     5701 gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     5761 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     5821 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     5881 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     5941 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     6001 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     6061 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     6121 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     6181 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     6241 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     6301 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     6361 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     6421 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     6481 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     6541 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     6601 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     6661 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     6721 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     6781 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     6841 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     6901 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     6961 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     7021 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     7081 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     7141 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     7201 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     7261 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     7321 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     7381 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     7441 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     7501 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     7561 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     7621 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     7681 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     7741 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     7801 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     7861 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     7921 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     7981 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     8041 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     8101 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     8161 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     8221 gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     8281 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     8341 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     8401 cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     8461 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     8521 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     8581 aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa acaactggag
     8641 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
     8701 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
     8761 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
     8821 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
     8881 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
     8941 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
     9001 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
     9061 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
     9121 tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata aacgtttttt
     9181 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
     9241 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
     9301 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
     9361 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
     9421 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
     9481 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
     9541 agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
     9601 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     9661 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     9721 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     9781 agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc agcgaatcgc
     9841 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
     9901 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
     9961 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
    10021 attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
    10081 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
    10141 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccaaa
    10201 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
    10261 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
    10321 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
    10381 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
    10441 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
    10501 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
    10561 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
    10621 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
    10681 gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga acctgagcgc
    10741 ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
    10801 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
    10861 atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg aatcgtaaag
    10921 aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa cggaatcact
    10981 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
    11041 ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
    11101 cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat gtaccgcata
    11161 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
    11221 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
    11281 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
    11341 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
    11401 cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga agtaacttga
    11461 ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat atattctgag
    11521 atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa aatatagatt
    11581 tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat gttgcagttc
    11641 tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc tatcttaacg
    11701 atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac aattcgcggc
    11761 tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg cacattttcc
    11821 ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat ataaaattgt
    11881 tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat gaaagtagga
    11941 aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt tcgtgcgaat
    12001 gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac agtacaaagg
    12061 gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt taaaagtttg
    12121 tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg agctaaaata
    12181 ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat atctacagag
    12241 agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac gtctttcaca
    12301 gaagttgcag gaaaattctc tttacatatt tttaatagaa aat
//
LOCUS       DAZKCE010000086        12341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000086 DAZKCE010000000
VERSION     DAZKCE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.087"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8KQ_002133"
     CDS             <1..154
                     /locus_tag="ACG8KQ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9105048.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8KQ_002134"
     CDS             complement(208..486)
                     /locus_tag="ACG8KQ_002134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105049.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8KQ_002135"
     CDS             complement(601..1218)
                     /locus_tag="ACG8KQ_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105050.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8KQ_002136"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8KQ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9105051.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8KQ_002137"
     CDS             2780..3196
                     /locus_tag="ACG8KQ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9105052.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8KQ_002138"
     CDS             3193..3663
                     /locus_tag="ACG8KQ_002138"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9105053.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8KQ_002139"
     CDS             3677..4450
                     /locus_tag="ACG8KQ_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9105054.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8KQ_002140"
     CDS             4464..5276
                     /locus_tag="ACG8KQ_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9105055.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8KQ_002141"
     CDS             5330..6295
                     /locus_tag="ACG8KQ_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9105056.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8KQ_002142"
     CDS             6431..9154
                     /locus_tag="ACG8KQ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9105057.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8KQ_002143"
     CDS             9167..9799
                     /locus_tag="ACG8KQ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9105058.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8KQ_002144"
     CDS             10007..10231
                     /locus_tag="ACG8KQ_002144"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9105059.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8KQ_002145"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8KQ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP9105060.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8KQ_002146"
     CDS             10556..10825
                     /locus_tag="ACG8KQ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9105061.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8KQ_002147"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8KQ_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9105062.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4032 a         1999 c         2422 g         3888 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca a
//
LOCUS       DAZKCE010000087        12188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000087 DAZKCE010000000
VERSION     DAZKCE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.088"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..312)
                     /locus_tag="ACG8KQ_002148"
     CDS             complement(148..312)
                     /locus_tag="ACG8KQ_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105063.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            607..1935
                     /locus_tag="ACG8KQ_002149"
     CDS             607..1935
                     /locus_tag="ACG8KQ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9105064.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2137..2970
                     /locus_tag="ACG8KQ_002150"
     CDS             2137..2970
                     /locus_tag="ACG8KQ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9105065.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2986..3723
                     /locus_tag="ACG8KQ_002151"
     CDS             2986..3723
                     /locus_tag="ACG8KQ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9105066.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3878..4846)
                     /locus_tag="ACG8KQ_002152"
     CDS             complement(3878..4846)
                     /locus_tag="ACG8KQ_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9105067.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5078..5911
                     /locus_tag="ACG8KQ_002153"
     CDS             5078..5911
                     /locus_tag="ACG8KQ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9105068.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5945..6784
                     /locus_tag="ACG8KQ_002154"
     CDS             5945..6784
                     /locus_tag="ACG8KQ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9105069.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6810..7370
                     /locus_tag="ACG8KQ_002155"
     CDS             6810..7370
                     /locus_tag="ACG8KQ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9105070.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7569..9290
                     /locus_tag="ACG8KQ_002156"
     CDS             7569..9290
                     /locus_tag="ACG8KQ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9105071.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9455..10597
                     /locus_tag="ACG8KQ_002157"
     CDS             9455..10597
                     /locus_tag="ACG8KQ_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9105072.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10613..11824
                     /gene="thiI"
                     /locus_tag="ACG8KQ_002158"
     CDS             10613..11824
                     /gene="thiI"
                     /locus_tag="ACG8KQ_002158"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9105073.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2046 c         2532 g         3457 t
ORIGIN      
        1 actgccggag taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt
       61 ttcttttatt attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta
      121 cgaaagagta gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa
      181 ataaatcagc attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt
      241 cccaaagtta tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat
      301 caggtttttc attttattca tctcctttct tacgttatag tatttccttc tttcaatgag
      361 tattttgaaa gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc
      421 cattttcgtt atctcataga aatattcctt tttatctgaa aactattgta taccgtatga
      481 catttttctt catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag
      541 agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat
      601 gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca
      661 aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct
      721 gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg
      781 aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc
      841 cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca aaattacact
      901 tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga
      961 aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc
     1021 ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg
     1081 atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta
     1141 ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct
     1201 attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta
     1261 ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt
     1321 gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat
     1381 tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta
     1441 gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa
     1501 actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt
     1561 gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg
     1621 gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa
     1681 agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca
     1741 aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg
     1801 agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg
     1861 aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg
     1921 ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg
     1981 gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct
     2041 ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga
     2101 cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct
     2161 gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat
     2221 cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga
     2281 ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat
     2341 acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg
     2401 ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg
     2461 ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt
     2521 gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca
     2581 gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt
     2641 gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg
     2701 agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct
     2761 tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa
     2821 tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg
     2881 atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc
     2941 caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat
     3001 accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct
     3061 tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc
     3121 agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt
     3181 aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat
     3241 aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa
     3301 atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa
     3361 gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt
     3421 gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt
     3481 aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac
     3541 tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac
     3601 gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa
     3661 catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa
     3721 taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa
     3781 ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca
     3841 gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca
     3901 attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac
     3961 tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat
     4021 aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact acagggattt
     4081 tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta
     4141 aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt
     4201 gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc
     4261 gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg
     4321 gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc
     4381 gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac
     4441 aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac
     4501 ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa
     4561 aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg
     4621 atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg
     4681 gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt
     4741 ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt
     4801 tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct
     4861 tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta
     4921 tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga
     4981 aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga
     5041 aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc
     5101 gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg
     5161 gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt
     5221 tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg
     5281 aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca
     5341 ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca
     5401 attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa
     5461 gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga
     5521 atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga
     5581 tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt
     5641 tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg
     5701 gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga
     5761 agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg
     5821 tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg
     5881 actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa
     5941 gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa
     6001 agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg
     6061 tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga
     6121 tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca
     6181 cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca
     6241 aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc
     6301 gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa
     6361 aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat
     6421 attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt
     6481 agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat
     6541 cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga
     6601 aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt
     6661 aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt
     6721 gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta
     6781 ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag
     6841 aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa
     6901 atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta
     6961 cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata
     7021 aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag
     7081 tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag
     7141 tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg
     7201 gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg
     7261 aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg
     7321 ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc
     7381 actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat
     7441 gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt
     7501 tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg
     7561 aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca
     7621 ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct
     7681 tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta
     7741 aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga
     7801 atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc
     7861 tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa
     7921 tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa
     7981 cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag
     8041 aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga
     8101 tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg
     8161 ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga
     8221 tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag
     8281 aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc
     8341 ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa
     8401 aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt
     8461 tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa
     8521 cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg
     8581 atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc
     8641 aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac
     8701 acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag
     8761 atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa
     8821 aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg
     8881 tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta
     8941 ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg
     9001 tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg
     9061 atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc
     9121 atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat
     9181 atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg
     9241 ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag
     9301 ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt
     9361 tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat
     9421 aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac
     9481 aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg
     9541 gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag
     9601 aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg
     9661 aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa
     9721 tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa
     9781 agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga
     9841 cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa
     9901 tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa
     9961 aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt
    10021 gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac
    10081 tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca
    10141 agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc
    10201 tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt
    10261 agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga
    10321 ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca
    10381 tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca
    10441 tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt
    10501 tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt
    10561 caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata
    10621 cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt
    10681 tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat
    10741 ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat
    10801 tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa
    10861 agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa
    10921 agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga
    10981 attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat
    11041 gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta
    11101 tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat
    11161 gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga
    11221 aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc
    11281 aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc
    11341 atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat
    11401 tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt
    11461 cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc
    11521 tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga
    11581 aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga
    11641 ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca
    11701 tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa
    11761 gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct
    11821 ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata
    11881 gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt
    11941 atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta
    12001 gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg
    12061 ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa
    12121 atttttgtga aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag
    12181 aggttgcg
//
LOCUS       DAZKCE010000088        12183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000088 DAZKCE010000000
VERSION     DAZKCE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.089"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..623)
                     /gene="frr"
                     /locus_tag="ACG8KQ_002159"
     CDS             complement(66..623)
                     /gene="frr"
                     /locus_tag="ACG8KQ_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9105074.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(626..1348)
                     /gene="pyrH"
                     /locus_tag="ACG8KQ_002160"
     CDS             complement(626..1348)
                     /gene="pyrH"
                     /locus_tag="ACG8KQ_002160"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9105075.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1484..2365)
                     /gene="tsf"
                     /locus_tag="ACG8KQ_002161"
     CDS             complement(1484..2365)
                     /gene="tsf"
                     /locus_tag="ACG8KQ_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9105076.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2464..3246)
                     /gene="rpsB"
                     /locus_tag="ACG8KQ_002162"
     CDS             complement(2464..3246)
                     /gene="rpsB"
                     /locus_tag="ACG8KQ_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9105077.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3605..4084
                     /locus_tag="ACG8KQ_002163"
     CDS             3605..4084
                     /locus_tag="ACG8KQ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9105078.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4301..5392
                     /gene="phnW"
                     /locus_tag="ACG8KQ_002164"
     CDS             4301..5392
                     /gene="phnW"
                     /locus_tag="ACG8KQ_002164"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9105079.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5385..5513
                     /locus_tag="ACG8KQ_002165"
     CDS             5385..5513
                     /locus_tag="ACG8KQ_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9105080.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5517..6062
                     /locus_tag="ACG8KQ_002166"
     CDS             5517..6062
                     /locus_tag="ACG8KQ_002166"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9105081.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6516..8207)
                     /gene="argS"
                     /locus_tag="ACG8KQ_002167"
     CDS             complement(6516..8207)
                     /gene="argS"
                     /locus_tag="ACG8KQ_002167"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9105082.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8627..9574)
                     /gene="arcC"
                     /locus_tag="ACG8KQ_002168"
     CDS             complement(8627..9574)
                     /gene="arcC"
                     /locus_tag="ACG8KQ_002168"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9105083.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9689..10708)
                     /gene="argF"
                     /locus_tag="ACG8KQ_002169"
     CDS             complement(9689..10708)
                     /gene="argF"
                     /locus_tag="ACG8KQ_002169"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9105084.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10799..12028)
                     /gene="arcA"
                     /locus_tag="ACG8KQ_002170"
     CDS             complement(10799..12028)
                     /gene="arcA"
                     /locus_tag="ACG8KQ_002170"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9105085.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3338 a         2528 c         2203 g         4114 t
ORIGIN      
        1 ataaaagaaa tataaataaa aacagaggaa gcggaatgat ccgcccctct gtttttatga
       61 ttttattaaa cttccaataa ttctttttct ttatcagcag cgatcttatc tacttctttg
      121 atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc atcttcggta
      181 atatcaccat ttttttgttg ttttttatat tcatcgatcg catcacgacg gatgttacga
      241 atcgcgattt ttgcattttc tgcttctttt ttcacatctt tagctaattc ttttctgcgt
      301 tcttctgtca gttgcgggat aactaagcga atgacatttc catcattggt tggactgatt
      361 ccgatatcac ttgccataat agctttttca acatcttgaa gaatgctctt gtcaaaaggt
      421 gtgatcatta aaacacgtgc ttctggaatt tggattgatg ccatttggtt caatggtgtt
      481 ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc tagcgttcgc acggcctgcg
      541 cggatttgcc caagttcacg ttgaagattt tgtgctgctt ttttcatttt ttcttttgct
      601 tctgtcataa ttgcatctgc catttttatt tcccccttac agttgttccg atattttcgc
      661 ctaagattgc acggcgaata tttccgtgtt catttaaatt gaagacaagc agtggaatat
      721 cattatccat gcttagtgaa cttgcagttg aatccatgac ttgtaatcct ttggcaataa
      781 cttccagatg agtcagttct tcaaatttta ctgcatttgc atctactttc ggatcagcag
      841 aataaacacc gtctacattg tttttagcca tcaggataac atctgcgccg atttctgccg
      901 cgcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg cctgcaaaga
      961 taacgatccg tcctttttct aaatgacgtt ctgctttacg gcgaatatat ggctctgcga
     1021 tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt tccaacgtat
     1081 cctgtaaagc taaggcattc ataactgtag ctaacatacc catgtaatca gcttgagcac
     1141 gttccatgcc catttgtgct ccgatttgtc cgcgccagat atttccgccg cccacaacga
     1201 ttgccatttc aacgcccagc tcgtgaactt ctttgatttc ttggatgatt tctttgatta
     1261 ctggtggttt gattccaaat cctgcgtccc cagctaaagc ttctccactc aattttaaga
     1321 ctacacgctg atatttcggt gtgaccatgt tcattcctcc aatattttct attgccattt
     1381 taccatactt agatgatttc gacactctaa gactggtttt tcttgttttg cttaaaaaaa
     1441 gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt ttcacttggc
     1501 tcataacttc ttctacaaag ttatcttcgc gtttttcgat accttcgcca acttcgaaac
     1561 gtacgaatgt tttaacagtt gcgccttttg atgcaacgta tttttcaaca gtcatatcag
     1621 gatctttaac gaatggttgg tccaccaaag cgatttctgc tttgaatttc ttcaagcgtc
     1681 cttctaccat tttttcaacg atattcgctg gtttgccttc gttcaatgct tgttctgtaa
     1741 gaacagtttt ttcatgttct aattcagctt ctggaatttg tgattcgttt acataacgag
     1801 gattgatagc tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca gttgttccat
     1861 ctagaacagt caatacggca atacgtccgc ccatatgcaa gtatccacca aatgcagcat
     1921 tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat tttttctccg ataacttgag
     1981 ttgcttcgat caagtcagat tcaattgtac ctttatctgt tttgattttc attgcagctt
     2041 ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat ttcttttact aaatcttgga
     2101 acatttcgtt tttagaaaca aagtctgttt ctgagtttac ttcaacgatt gctgcagtgt
     2161 tacctttcac agcaactgaa gccaatcctt cagctgcaat gcggtcattt ttcttcgcag
     2221 cctttgccat tcctttttca cgtaaaagat caactgcttt ttccatgtcg ccttctactt
     2281 caactaatgc ttttttcgca tccatcattc ctacgccagt catttcgcgt agttctttta
     2341 ccattttagc tgtaacgtct gccatgttta actcctcctg tgtgtttttc tttttaaaaa
     2401 aagctgcttc aaaggagagt gaggcttgtc gcccagcctt tgaaacagct ccatcattca
     2461 aaattattct gctgaagaat tgtcgccttc aacaacatct acgatttctt cgattgatgt
     2521 tgctgtgttt tcttctgtga aatcttcttc aactacttga tcttcacctt ggtttccttc
     2581 gatgaaagca tcagccattt ttgaagtaat caatttaacg gcacgaattg catcgtcgtt
     2641 tgaagggatt actacgtcga tttcatctgg atcgcagttt gtatcaacca tagctacgat
     2701 tgggatattt aatttgtgtg cttcttgaac agcaatacgt tctttacgtg ggtcaacgat
     2761 gtacattaca tctgggattc ttggcatatc agcgataccg cctaagaatt tttcaagacg
     2821 ttcacgttct ttgttcaaac cagcaacttc ttttttaggt agaacttcaa aagttccatc
     2881 ttcttccatt gcattgattt gtttcaaacg gctgatacgt ttttggatag tatcccagtt
     2941 tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac gagttgcttc
     3001 ttctttgatt gcttcttgtg cttgtttttt tgtacctacg aataatgcta cgccgccttc
     3061 ttcagcaacg ctcttcatgt aatcataagc tgcatctact aatttaactg ttttttgcaa
     3121 gtcaatgatg tagattccgt ttctttctgt gaagatatat ttcttcattt ttggattcca
     3181 gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt aattgtttca tagaaattac
     3241 tgccatgttt gtttcctccg atttggtttt atttttcctc ttcttatctt ctaccttgcc
     3301 ggcaaacttt ttcaagcacc taccgacaat ccaataagaa tgtggatttg ctgatgcata
     3361 aagcaccctc agctattata cagacaaaag atccgtatcg caagagcaaa tatacttttc
     3421 tgctcaacat tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa cagtagattt
     3481 ctctttgttt ctttattatc ctttttccat aaaataattt taggtataaa agacgaatat
     3541 tattgggcaa tccttctttt ttttgttatg atacatgtga aatctttcgt aaaggagaaa
     3601 gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa gtcattgaag aattcccgat
     3661 ccgtacgcaa gaagaactat taaacaaact tgggcattat ggcgttaccg ccacgcaggc
     3721 aacgatttct cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt
     3781 ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag actaggactg aggaagagga
     3841 tcggctggtt caaatgattg aagacattgt attgaaagtc gaacgagtcc actttttgac
     3901 gatcgtcaat accttgccag acaatgctca tttatttgca tctgttttag atgagatcaa
     3961 acctccctct attgtcagta caattgctgg attcgatacg atcatggtca tctcggaaaa
     4021 tgaggaggac gcaaaacgag tagaagaatt tcttcaccat ccaactgaag tatcactttt
     4081 ttaatcagag cacgtattcc taatatggaa tgcgtgtttt tttattttca atattttcct
     4141 tattgaataa acctttacac tctttacaaa aatacatcat tttttattca aatttttcaa
     4201 caccttgatt gaatcacaat aatcatcatt tatattggat gttaacaaga gcagcttctt
     4261 gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat atgtataaat tattaacttc
     4321 tggaccattg acgacttctg aaacagtgaa aaatgctatg ctgatcgatc attgtacttg
     4381 ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa aaattattgg atcttgctca
     4441 agtatcggaa ggaatataca caacggtctt aatgcaaggt agcagctcct tcggggtaga
     4501 atcggtatta agttctgtga tcaggccaga agatactgta ctgatttgtt caaacggcgc
     4561 atatagacaa cgaatgatca acatgtgcga atgtctgaaa ctgaactata ccgtttatga
     4621 ggtatcggaa catcaaatcc tccaagctca tgtgattaca cagctgattg aagagaatcc
     4681 gtctatctcc gcactagcaa tggtccatag cgagactact tccggtattt tgaataattt
     4741 ggaagagatt gctgctgtca cacaagaaag agatcttttg tttatagtgg acgcgatgtc
     4801 aagctttggt gggataccga tttgtgtagc cgaactaggg attgattttt tgatttccag
     4861 ctcaaacaaa tgtattcaag gtgttccagg cttttctttt gtacttgcta ataaagcgga
     4921 actggaaaaa cgaaaaaatg ttagccgaag tttgtcactt gatctatatg accagtaccg
     4981 cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc cctacccata cggttttggc
     5041 ttttcaacaa gcgctgaagg aactagaggc agaaggagga atcgaaactc gatattcacg
     5101 ttatctcgcc aataatcatg tgctacgaca aaaaatgaca gagatggggt tccagccttt
     5161 cattcaagaa tatcaggggc cattcatcac ctcttttctt taccctaagg actggcgatt
     5221 tgattttaat catttttatc agtatttaaa gcaaaatgga tttgtgatct atcccgggaa
     5281 aatcagcgat catgaatgtt ttcgtatcgg aaatattggc gagatctatc cggaagatat
     5341 tttggaactg acagcaatta tcaaagacta taaggagaac gttaatgatt aaaactgtaa
     5401 tttttgattg ggcaggaaca actgtggact ttggctgtat ggctcctgtc cacgctttta
     5461 gaaatgcttt tctagaaaaa ggaacccaac tgactgacaa agaaatccgc taaccgatgg
     5521 gaaaattaaa atgggatcat atccaacagc ttctttccct cccttctgtc aaaaaacaat
     5581 ggatagctat ttatggacaa ctacctcagg aaaaagaggt ggaggaattg tatcaacaat
     5641 ttgaacatta tctgtttaaa gatttggtca agcattctac cttgaaaccc gacacattgg
     5701 aaacgatcga acggttgaga aacgctggga tcaatattgg ttccacgaat ggatttaatg
     5761 ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga aaggtattca ccagacttca
     5821 tggcaacttt tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg atccataaaa
     5881 acatgcagta cttcaaaaac aagtcggttg atgaagtaat caaagtcgga gatacggtct
     5941 cagatatcca ggaagggaaa aatgccggag tgttgacagt aggtgtaatt gaaggtagtt
     6001 cgctaatggg gatttcttat gatgagtacc aaaaattaga gatggggaaa aagatcgttt
     6061 aaaacagtct gttgctctta gattccaaca agccggcgct gatatttgtg taaacaattt
     6121 aaaagagtta acagaataca ttttgtacgc tgacaagaat ccagaatgac ttttatacaa
     6181 aataagaggt tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca agcaaaaata
     6241 gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
     6301 tttattcgaa aaaaaaaagg gagctgtgac acgacttttg tcacagtccc cttatttgca
     6361 aacaaacagt gtttaagaga caacctctcc actaaacggt gttcattcag ctgactctcg
     6421 gtatttgctc aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt tctctaatac
     6481 tcggatcaga acgctgattt cacattctct ttttcttaca tcttatctgg tgcatgaagt
     6541 cctagcaaac gaaggtcttc tttcaataat acagtcactg cataaactag agctaagcgt
     6601 gcttcttttt gttcatcatc agctaaaatc ttcgtatgag catagtattt gttgaatgct
     6661 tgtgctaatt tgattgcatg tttcgcaatc actgacggtt cgtatttttc cccagcagac
     6721 aagaccgttt cagggtattt ttgaactaat ttaaccactt cccagctggt atcgtcattc
     6781 aatgcatagt ctgctgtttc tgaagggaca aatcctgctt tttccaacag gctgactgca
     6841 cgagcatgtg tatattgcac ataaggtcca gtttctcctt caaatcgaac aacttcttcc
     6901 aggttaaagt caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat gactgcccca
     6961 accccaactt gtttagctac agcatctttg ttttccaaat cagggttttt ctcgctgatt
     7021 tgttctttag cagattggat tgcttcattt aatacttctt ctaataagac gatctttcct
     7081 ttacgagttg ataatttttt accaccttgc gtgatcaaac caaatggaat atggtgcatg
     7141 tcgtcagacc agtcaaagcc catttccttc aagacagctt tcaactgttt aaaatgatag
     7201 ctttgttcat tccctactac atatagagat tgtttgaagt cgtaagtacg tttacggtac
     7261 aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac
     7321 gctggattca ggtcataagc agataggtca acgatctctg cacctttatc ctcgttcagc
     7381 aaatgttttt cttctaaaag tttgacgatt tcatccatct tgtcattgta gaaagcttca
     7441 ccgttcaacg aatcgaaacg tacttccagc agatcataga ttttattgaa ttctttcatc
     7501 gattcatcac ggaaccattg ccataattga atagcctctt tatcgccttc ttccagacgt
     7561 ttgaaccaag cacgagcttc ttcatttaat tctggttctg tttcagctac ttcatggaat
     7621 tggacgtata aacgaagcaa ttcattgatt ggttctgctt taacagcttc ttctgtaccc
     7681 cattttttgt aagcgacgat cagttttcca aactgtgttc cccagtcacc taaatggttg
     7741 atgcggattg gctgataacc gattttttcc ataatgaaac cgatcgaatt accgataact
     7801 gtcgaacgca aatgtcccat tgagattggt ttggcaatat taggagaaga catgtcgatt
     7861 ggtactgttc cttgattccc gatgttgcta tccccataat gttctttttc tttcactact
     7921 gtttgcaaaa cttttttgct gataagttct ttattcatga aaaagttcaa gtaaggtccg
     7981 acaacttcga ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt
     8041 tgctgtggcg cttttcggta aacttttgct aatgaaaagg ctggaaatgc aacatctccg
     8101 tgttcagctg attttggatt ttctagtaat tgctcgactt gttccagtgt taaatcatct
     8161 ttgacagcgt catagacagc ttttgcgaca agatctttat tattcatctt tattcctcct
     8221 aaagtgttcc cttgattgtt aaacaaaaaa aatctctagc gacggcatac ttagtatgcc
     8281 atcactagag acgagatttc ccgcggtacc actccaattg tcctaaaaaa ggaccagctc
     8341 catctgatat cggtagacga ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg
     8401 tatcttcttc attcggcttc caccacttcc gaattcgcta gccaagaatg atacaagtac
     8461 tctcttgttc aacaaatctt agtcttatta aactttcttg aattatatca gaaaaaaaaa
     8521 cgaaaggcta gtcgtttcat aaaaaaaaaa tccagatggc ctaaaggaga agaaatacca
     8581 cctggatagc tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga
     8641 taatcgttcc ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg
     8701 ccttagcgtt tggttttgct tcaacaaatg caatagctgc ttctactttc ggaagcatgc
     8761 ttcctggagc gaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt
     8821 ctaatttctt ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata
     8881 cgatcagcaa atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga
     8941 ttacggcttc tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac
     9001 caacagagac tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa
     9061 tcgagatggg tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga
     9121 atgtcacagt ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac
     9181 cgactggttt acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga
     9241 ctaaagaaac cacatcttta tcgatccctt tttccttcag tacttcgccc atggcgtttt
     9301 gtaaccagta accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg
     9361 gtgttttctc agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat
     9421 ttccgtggga gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg
     9481 tttccattaa cgcttcttgt tgggcttttg cacttgcatc cgtagacaag atggcattgc
     9541 cccctaaagc gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac
     9601 cgaattttct agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg
     9661 ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg
     9721 ttgctgccat gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag
     9781 catatttgct gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt
     9841 tcatttgttc accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga
     9901 tcaaacgatc tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca
     9961 acaatttgat acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat
    10021 aaaggacgtc tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac
    10081 cggatttttc agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt
    10141 ttggtgcaac gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag
    10201 ccatattgtt tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt
    10261 tttcttggat agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc
    10321 cattccaaac tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc
    10381 cacggaattc gattccgtca aacatacgtc ctagtacttt agctgtatct tctgtggatt
    10441 cttttttacc taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga
    10501 tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa
    10561 tattttttcc ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt
    10621 ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa gtctttttct gctaataagc
    10681 ttcttccttg gaatactgat tctttcatat taaacattct cctttagatt gtttttattt
    10741 aagattaaag aggaaatccc gacggcagct gacagtaaga ctgccgtcag gaaacaaatt
    10801 acttcaagtc ttcacggaca agtggttggc tcatgcagcg tggaccacca cgacctcgtg
    10861 acaattcact tgaattgatt tctaacactt tgatgccata gctacgaagc aattcgttcg
    10921 atacataatt tcggttataa gtgactacaa cacctggagc gattgccaat gtgtttgatc
    10981 catcattcca ttgttctcga ggtgcaacga tctcatctcc attaccagtt gggatcagta
    11041 caagatcatc taatccaaga gcagctttca tcgtttcgtg taagttatct gaatgagtaa
    11101 tcttgatatc atcaccatct ggttcgattg taaagacatc catcttaccg tctttacttt
    11161 gaattgctgg atggatagtg aatttatcgt gatctaccat tgtaaatacg gtatccaaat
    11221 gcatcattgc acggttgttc ggaattttga ttgccaacac tttttcaaat cctgaatttt
    11281 ttgcgaatag gtggcgtgct aatttttcta aagctttcgc attagttcgt tgagaaatcc
    11341 caacagctac tactttatcg cttaagatca attcgtctcc gccttcgata tgatctggat
    11401 tttcacgatc caaccatact tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa
    11461 tatattctgt aaacatcgat tcacgacgac gtgcttcaaa agtcatctta ttgactgtca
    11521 tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta caagtttggc attgggtcca
    11581 tgtaaaatgg atagtcatcg tctgctgaca gatcatatag actgctagaa cgtacttcga
    11641 tatcttttgt acgaacccca gccatgattt tatctaccat ttcttgcgtt tccattgata
    11701 gaaggaactc atgaagaccc tctcttacag cattcgatgc aatatgagat tcatccaaca
    11761 ttttattcaa gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt
    11821 ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt cgcaaagttg tcatgttctt
    11881 cttgagcaat cggaagatat ggaatatcat cgaacaacag acgatccata atatctggtg
    11941 tcaaattttc cacttcttgt ccaggccgtt tcagtaaaac tgtctttaat ttccctattt
    12001 cagagaaaac gtgaataggt ttatccatgt tgtgttgcct ccttcttttt gattacatgt
    12061 ataggatatc ctccttttgt aatcactttc ataattttgt ctcattcaaa atgagacatt
    12121 cctcacatta atattcgata attaaataat taaacgtttt tctattttta agcgtaattt
    12181 att
//
LOCUS       DAZKCE010000089        12103 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000089 DAZKCE010000000
VERSION     DAZKCE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.090"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..350
                     /locus_tag="ACG8KQ_002171"
     CDS             156..350
                     /locus_tag="ACG8KQ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105086.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            347..841
                     /locus_tag="ACG8KQ_002172"
     CDS             347..841
                     /locus_tag="ACG8KQ_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105087.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1003..2175)
                     /locus_tag="ACG8KQ_002173"
     CDS             complement(1003..2175)
                     /locus_tag="ACG8KQ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9105088.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2381..3196)
                     /locus_tag="ACG8KQ_002174"
     CDS             complement(2381..3196)
                     /locus_tag="ACG8KQ_002174"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9105089.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3346..3792)
                     /locus_tag="ACG8KQ_002175"
     CDS             complement(3346..3792)
                     /locus_tag="ACG8KQ_002175"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9105090.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3789..4304)
                     /locus_tag="ACG8KQ_002176"
     CDS             complement(3789..4304)
                     /locus_tag="ACG8KQ_002176"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9105091.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4377..5291)
                     /locus_tag="ACG8KQ_002177"
                     /pseudo
     CDS             complement(4377..5291)
                     /locus_tag="ACG8KQ_002177"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5288..7615)
                     /locus_tag="ACG8KQ_002178"
     CDS             complement(5288..7615)
                     /locus_tag="ACG8KQ_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP9105092.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8224..8370
                     /locus_tag="ACG8KQ_002179"
                     /pseudo
     CDS             8224..8370
                     /locus_tag="ACG8KQ_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8458..8751)
                     /locus_tag="ACG8KQ_002180"
     CDS             complement(8458..8751)
                     /locus_tag="ACG8KQ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP9105093.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            8788..8973
                     /locus_tag="ACG8KQ_002181"
     CDS             8788..8973
                     /locus_tag="ACG8KQ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105094.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(9154..10332)
                     /locus_tag="ACG8KQ_002182"
     CDS             complement(9154..10332)
                     /locus_tag="ACG8KQ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9105095.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10436..10750)
                     /locus_tag="ACG8KQ_002183"
     CDS             complement(10436..10750)
                     /locus_tag="ACG8KQ_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105096.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10857..11072
                     /locus_tag="ACG8KQ_002184"
     CDS             10857..11072
                     /locus_tag="ACG8KQ_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105097.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(11372..11614)
                     /locus_tag="ACG8KQ_002185"
     CDS             complement(11372..11614)
                     /locus_tag="ACG8KQ_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105098.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(11601..12029)
                     /locus_tag="ACG8KQ_002186"
     CDS             complement(11601..12029)
                     /locus_tag="ACG8KQ_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP9105099.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         3510 a         2363 c         2107 g         4123 t
ORIGIN      
        1 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
       61 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
      121 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
      181 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
      241 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
      301 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
      361 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
      421 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
      481 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
      541 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
      601 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
      661 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
      721 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
      781 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
      841 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
      901 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
      961 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     1021 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     1081 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     1141 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     1201 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     1261 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     1321 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     1381 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     1441 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     1501 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     1561 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     1621 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     1681 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     1741 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     1801 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     1861 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     1921 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     1981 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     2041 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     2101 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     2161 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     2221 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     2281 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     2341 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     2401 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     2461 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     2521 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     2581 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     2641 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     2701 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     2761 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     2821 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     2881 tttctacagt aatcgtgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     2941 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     3001 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     3061 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     3121 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     3181 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     3241 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     3301 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     3361 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     3421 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     3481 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     3541 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     3601 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     3661 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     3721 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     3781 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     3841 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     3901 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     3961 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     4021 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     4081 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     4141 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     4201 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     4261 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     4321 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     4381 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     4441 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     4501 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     4561 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     4621 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     4681 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     4741 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     4801 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     4861 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     4921 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     4981 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     5041 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     5101 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     5161 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
     5221 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
     5281 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
     5341 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
     5401 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
     5461 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
     5521 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
     5581 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
     5641 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
     5701 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
     5761 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
     5821 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
     5881 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
     5941 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
     6001 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
     6061 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
     6121 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
     6181 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
     6241 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
     6301 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
     6361 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
     6421 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
     6481 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
     6541 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
     6601 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
     6661 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
     6721 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
     6781 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
     6841 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
     6901 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
     6961 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
     7021 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
     7081 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
     7141 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
     7201 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
     7261 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
     7321 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
     7381 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
     7441 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
     7501 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
     7561 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
     7621 ctctcaataa aaaatcatca aataatcctt tacacgttat tctaaattta atttttatta
     7681 ttttattgat gtatagaaag tttggggctt tttttctctt tttattctaa ggttatttta
     7741 gtcttgtcct ttaatacaaa gattttattt ttaaataaaa aatactcttt ttttacgatt
     7801 attgaaaaaa aaagataaaa cttgattaat tgaagtattg cttaaatttg gaagctatga
     7861 gggaagaata aaagagttag gacgggatta tctactattt ttctttgttt taaatatttg
     7921 ttatctttga acataaaaga gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat
     7981 aaacggtgtt caactagctg atcttgtatt cacttaaaaa atggtaaagt acgagttgct
     8041 attccccatt ttcaattttc taacgctcag gacaaaatac tgttttcaca acctcattgg
     8101 aataaaagca tatatattgt ttcaagaacg aaatcttgtt atctttttaa agggcatctg
     8161 tccaaaatct aattttagga ggtacgtttt ggtatttatt cgtacaaata aaagagaatt
     8221 atctttattc atgtaacgca tccaattgat tttttcatgt acgctcctgg aacgatttta
     8281 ctgacgattg cttatttaac tgcgaatcgc tcattagctt ttatcatagc tatccatatt
     8341 ttgaataacg ttcttggctt tgtattatag atacagatga caaaaaaagt tcaagtatgc
     8401 ggtgtagaac caatatcgca tacttgaact tttttcaatg agtataaatt aaaacttcta
     8461 attttcaatt cccatcatct gatatgcccg accgtctgaa cttcttttga aaaagccaaa
     8521 tgtgattaga taacgtcttt gttcgacata atcgccacta gaaaatacgt tcagcagtat
     8581 cgaattaacc tctgtttctg tataagtcac tccatggtca aagcgtttca agacttcttt
     8641 caataggatg tatttctttt ttgttgaact tggtacttgt tttacttttc catcattcat
     8701 gaatgtgtct aaaactttca tcttttcttg ctccgtataa gtataaacca tcttctttct
     8761 ccttttgatt ctaagaatac tccatttatg ttagaatatt ctggattttt tattaaagcg
     8821 gttactctta agaaagatat tatcatagtg ctcttttttt tactactact tataggtaga
     8881 tttattctaa tccattattc tcccctacca ataattaaag aaaaaactgg atcagggtgt
     8941 tcaaattaca tgctaagaaa aaagaaccct tgaataccaa gggttctttc tcagtctgat
     9001 aacaatgatt aacgacggat ttctttgata cttgtgaaaa gtcgttaaag tattgatata
     9061 aaaggatttt tattacctct gactacatat tgactaattt gttaaaaaat cgtttttcaa
     9121 gaatcctgta ataccagtgc tttcaccgtt tttttaactc tctaaagaac taaaagcaat
     9181 cagaggtttt accttttcat ttgacgttaa gtggcgataa attttcgaag ttgtttggtc
     9241 agtatggcct aagcgatctt tcaagacttc ccagctacag tgttccgcta ctaagcttgc
     9301 cattgtatga cgagctaaat gagtatgaaa tggcttatta attcctgagc gattcacgca
     9361 ccgtttgacg tgatcattta catactctcg acgataagga acaccacgtt tactagaatc
     9421 aagatacaca aacaaatatg tttcttctat aataaacctg taatgttgcc tcatctcacc
     9481 caaagcctta atcaactgtt gtagcttttc cataactaga ggagttacta gaactcgtct
     9541 agcagaacta actgtcttag tagtttcaat tcgaaaatcc ttactcttac ctttaactgc
     9601 cgtttgctta tagaacagaa tttcatttct tttgaaatct atatctccag gctgtagagc
     9661 aagcgcctcg ctgactcggc agccagtgta aaatagaaca tggaaaagta cagacatcgg
     9721 taattcctca tgaacaatgc cataattcaa aaaagttctc aattcatcga cagtaagaaa
     9781 cttgtcctca atattattta tggcttcttt taaatcagtt accgttaact gatattttgg
     9841 cattctcaca gacttcattg gattttcact tatcaaattc tttctttgac aatatttaaa
     9901 aatcatattt agaacttgaa taagtgattg taaactattt ttggcatagc caacatttgt
     9961 tccattaacg ccacattcct ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa
    10021 atcagacaac tttttatcac caatccatcg aacaataatt tttaattgtt cctctcgctt
    10081 atcataggtt atttcctttt gttcatttaa cgaacggtaa tattggaacc attgctcacc
    10141 tacaacacta aacttgtcat tgcttgcaac aatttttttc ttaagccttt gttgtttctc
    10201 catttccaga taagcgagtt gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg
    10261 atatgcttga aagtaccatg tgatcctacc agtagaactg atttgcttct tgaatcgatc
    10321 tgttttttcc atttattgat tcctcctatt ttgaaggaac ccaacaagca gaaacccttt
    10381 ggaaatcttt ctatctatgt catcattgta ccgcatctta gaaagaaaat gtaacttata
    10441 aagatcgaag tagtcgcaat ttttctcgat agtcattcga atcactcttt ctcatccatt
    10501 ctaaaacatc atcacgaaaa tagtgtcctc gaatcccgta ccaacaggga ggaaactcac
    10561 caagttttac taaacggttc agtgtactat cacttatctg aaatagtcgc tttaaatcag
    10621 tatttgttaa taccagaggt aaatgactga ctgcaagcaa tttttccgtg taatcttcca
    10681 gttttttttg taaaattgaa tagaactcta tttccagaga attaacattt gttttaactt
    10741 ccaactccat cacccttctc tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt
    10801 gtaaaaaaac actccagtac tccatcaaaa cggactagaa aagaggaatt tttaatatga
    10861 aacaaggaat tgcagacata aaaataatta aagaaattct tgaaaaatct acggctaatg
    10921 caatagcatt cggaacagga atcaacttga gtacagtaaa aaaacttaaa agtggagaga
    10981 gagctgaaga aaaattaaat cttgccgatg ctattaaaat cacagaattt ggcatgaaaa
    11041 atatgccaac caaaatcgaa atatggaaat agtagttttt ctgatctatc agctgttttg
    11101 ccaaagcaca taggaatctc acctcccccc agtaatggcg ggccgatttg gttacggaag
    11161 tatcattatc gtagtcgcga ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc
    11221 atggcgtaac tagagaatca aaatctacat gtactgatag atttgctatt catttgtcaa
    11281 agagcgctag gctattttca gcctatggaa atcctttatt ttgacccgat aaaaaacttc
    11341 catagaatga aaaggaaagg gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct
    11401 tctcaatcaa ttttgtttcc agttgtctac gcatatattc atctacatac ataaccattc
    11461 ctttttcttc ttcataaact ggtttcatgg ctaaccgtga aatataattt tgatattttg
    11521 acagaacgat ggacatggca attgaatcac cagaagctgc ggctttgatt gtcgttagtg
    11581 gtatgaatgg tatatatcgt ttattttgct tcatatttga cacccaaata ctttcctagc
    11641 tcttccaaag ccatttgtcg ttggtaccaa acactgccga gacttttccc aataatatcg
    11701 ccaatttcac gatcattaag accgatgaag aaggacataa aaatgatttc ttgtctttga
    11761 ctaggtaatg ctgcaatcgc atttgccaac tcctcattgg aaataaagat tttcatacca
    11821 aggacaataa aagtaccact actattcaaa tttggttcca aataatagaa ctctgtacct
    11881 aacccttttt gttccaatat ttccattgat atttgccgtt ggcgaaatct agcgtacttc
    11941 ttttgaatat ttaacgcctc attgcagact actttcttac aaaaactgtc aaacgtatgt
    12001 tgaatgtggg tttcctctga gtcgcccata atattctccc cctcaaaaaa ttcattcaac
    12061 tcgttttact tctatccctt tccattatta ctacaatcta gga
//
LOCUS       DAZKCE010000090        12001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000090 DAZKCE010000000
VERSION     DAZKCE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.091"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(123..2198)
                     /gene="glyS"
                     /locus_tag="ACG8KQ_002187"
     CDS             complement(123..2198)
                     /gene="glyS"
                     /locus_tag="ACG8KQ_002187"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9105100.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2200..3117)
                     /gene="glyQ"
                     /locus_tag="ACG8KQ_002188"
     CDS             complement(2200..3117)
                     /gene="glyQ"
                     /locus_tag="ACG8KQ_002188"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9105101.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3503..4321)
                     /gene="recO"
                     /locus_tag="ACG8KQ_002189"
     CDS             complement(3503..4321)
                     /gene="recO"
                     /locus_tag="ACG8KQ_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9105102.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4464..5363)
                     /gene="era"
                     /locus_tag="ACG8KQ_002190"
     CDS             complement(4464..5363)
                     /gene="era"
                     /locus_tag="ACG8KQ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9105103.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5378..5773)
                     /locus_tag="ACG8KQ_002191"
     CDS             complement(5378..5773)
                     /locus_tag="ACG8KQ_002191"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9105104.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5757..6233)
                     /gene="ybeY"
                     /locus_tag="ACG8KQ_002192"
     CDS             complement(5757..6233)
                     /gene="ybeY"
                     /locus_tag="ACG8KQ_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9105105.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6262..8454)
                     /locus_tag="ACG8KQ_002193"
     CDS             complement(6262..8454)
                     /locus_tag="ACG8KQ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9105106.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8478..9449)
                     /locus_tag="ACG8KQ_002194"
     CDS             complement(8478..9449)
                     /locus_tag="ACG8KQ_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9105107.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10154..11533
                     /gene="brnQ"
                     /locus_tag="ACG8KQ_002195"
     CDS             10154..11533
                     /gene="brnQ"
                     /locus_tag="ACG8KQ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9105108.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11815..>12001
                     /locus_tag="ACG8KQ_002196"
     CDS             11815..>12001
                     /locus_tag="ACG8KQ_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9105109.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3282 a         2555 c         2192 g         3972 t
ORIGIN      
        1 aaattcgaac aaagaataaa ataacaaaaa ttattaaaat agaattaata acagctattt
       61 ataacaaaaa aaacagagac acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa
      121 gactattttg tgatgatttg atccaagctt gctaatgaca aagccatggc agctatttga
      181 cttagttgtg ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga
      241 tcaaaataat tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc
      301 gtttggtcag caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg
      361 tcgttttcga acagctcagg gtctacttct gattgttttt caagctgcgc ttttttcgct
      421 aagttcacga cacgtgtcaa agcttccatt gaaggtttga agtcttcatc tgcaagatgt
      481 tgtttgaaca attgtgctgc tgcaaatact tttgtcagat ctttttgttc cgcattcaaa
      541 acagcatcga tgacatcatg acgaatattc ttcgttgtca ataattggcg cagacggcct
      601 ttgataaagt cgatcacttg ttgttgccct tctgataaac caatgccgaa acgatccgta
      661 tctttactga taacatcgct aatcgtttct tgcagaacag acaaaggaaa ggcccatttt
      721 ttcgattcga tgatccgaac gacaccgtat gcttggcgac gtaaggcata aggatcgttt
      781 gacccgcttg gaatcatccc aactgcaaag aaagaaaaga tgctatctaa tttatctgca
      841 atcgctaaaa cagaacctac tgtagactga ggtagctctc cttcactgga tataggcatg
      901 taatgctcac gaatcgctgt cgctacagca ggtttctcgc ctttcagaag agcatatttt
      961 tcgcccatga tcccttgtag ttcaggaaat tcccctacca tgttggttac tagatcaaat
     1021 ttatagattt cagcagctcg ttgcaaatcc atcaattctt cctcagatag tcctacttgt
     1081 tcgccaatga tacgggcaat gacttgtacc cgttgcattt tctcatagat actaccgatt
     1141 ttttcatgga aagtaacagt ttttagtttt tcaacgtatt ggtcgattga agctttcttg
     1201 tcttcattat aaaagaattc cgcatcttct aaacgagccg tcaatacttt ttgattgcct
     1261 cgaaccacat cttccaattt tacccgatca ccattacgag cagaaataaa atgaggaagc
     1321 agcatacctt gatcattccg tacctcgaaa taacgttgat gttctttcat agaagtgact
     1381 agtacttctt caggaacaga aagatatttt tcttcgaagt cgccagaaaa gacagttgga
     1441 tattcgacta aattgttgac ttcttctagt aaatcttcgt ctagatcaat aacccagtga
     1501 tttttttctg ctaattcgtt tgcttgtgta acgatcattt cttgtctttt ttgcggagac
     1561 gcaatgacga attgttcatt caatttttct tcatattctt ttgcatgcgc aaatgtcaca
     1621 tcttcgccta agaaacgatg acctcgagag gctctgcctg ttttgacatc caagatttcg
     1681 aatgggatta cttcgtcatc taacaatgcc acgatccagt gaatagggcg gatatattca
     1741 aaatcgtatt ctgcccaatg catcgttaca gggaaagtca aacttttgat cacccctgct
     1801 aagcctgtca atacttcttt tgcttcctta ccatgagtgt atttcgttac atagacatat
     1861 tccacacctt ttaattctct aaaagtgata gcgtctgtcg tcagaccttg tccgcggaca
     1921 aatccttccg ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt
     1981 cctttgactt cttcttcact gtccgcttgt cgttctggaa tatttgttac tttgattgct
     2041 aaacggcgcg gtgtggaaaa tgtttcgatc gtatcataag tcaattggtt ttcatctaaa
     2101 aattttgctg ttttttcttc taattgtttc atgcttggtg tgacaacatg ggcaggcatt
     2161 tcttccagcc cgatttcaag taataagtct tttgccatct tattcagcct ccttcacggt
     2221 ttctggattt gctgtgtttt tattcaacaa agggaatcct aatttttcgc gttctgccac
     2281 aaagattttg gctacagcac gagccatgtt gcggatgcga gaaagataac cggcacgctc
     2341 tgttacggat actgcaccgc gggcgtctaa taagttgaat gtatggctac atttcaagat
     2401 ataatcatac gccggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa
     2461 acgatcaaag ttgcttagta gcaagtcttg gtcgcttact tcaaaagaat atttggaatg
     2521 ttcatattct ggttgtttga agatctcgcc atatttcacg ccatcagccc attctaaatc
     2581 atagacactt tcaacttctt ggatataaga agcaagacgt tctaatccat aagtgatctc
     2641 agaagttact ggtttacaag gcagtccacc aacttgctgg aagtacgtga attgtgtgat
     2701 ttccattccg tcaagccata cttcccaacc cacaccggca cagcccatgg atgggttctc
     2761 ccagttgtct tcaacaaaac ggatgtcgtg ttctaatgga tcgattccta aaaggcgcaa
     2821 gctttctaaa taaagctctt gaatattctc aggtgaaggt ttcatcacca cttggaattg
     2881 atgatgctga tacaaacggt ttgggttttc accataacgt ccatcagctg gtcggcgaga
     2941 tggctctacg taagcagcat tccatggttc tggtccgatt gcccgtaaaa aggtatatgg
     3001 gctcatcgta cctgctcctt tttctgtgtc ataagcttgc atcagcatgc agccgtttga
     3061 tgaccaaaat ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg
     3121 ttttgcccct ttcaatttta aagcgttgct gtcaaataag aatacaggaa agaaaacgac
     3181 aaaaaatccc tatgtctgat tacagacata gggacgaata attcgcggtt ccaccctact
     3241 tccgaataaa gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt
     3301 ttgggtccgg ctcccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt
     3361 caatgtacgt attcattttg cagttttctt gtttctataa tagatcctat cactgaacga
     3421 tctatttaat ctatggttaa taatagacgg aactccctgt gtttgtcaaa tggttttgat
     3481 tttttgttgt atgttttatt tactattctt ctttttcagg tgtctctttt tctttttcag
     3541 acatgtcttt ctctgacgtc tttaatacgt gtgcccaaga cttcatttga ttgatgaact
     3601 ttttgctttt caaatggata ccgacatatt catcatacag ctggtcgatg agttcacgga
     3661 ttgcttgttt ggtttcttct ttcagatgga ttccactaat tttatcataa gaaatagctg
     3721 aaaacatccg aacgaaataa acagctcgag gatctgcatg ataacggtga tcgtccatcg
     3781 accaatgtct ttcgcatatc acaccaccat atttggaaga atagtcaaat tttccttttg
     3841 tttccccgca tacgacgcag tgtgtccaga ttggtgcaat tccgaagcgt tgcagtattt
     3901 ggacttcaaa aatattcgtg atgatttctg catcgtctcc ttggtccatc cgttgcagtg
     3961 cttgttgcgt aaactggaac aaatgcgggt cgtactgatg atcttcgatt gccgcatcta
     4021 ctagattcaa taaatatgtg ccatagccgc tcaaaaagat gtcttcttgg atgtgtcgaa
     4081 acgcatggac atctttgaca ctgcttaaaa atgacaaacc ttcacttcga aaattcccga
     4141 tataaactgc ttcagtaaaa gggagcaaag ccggagctaa gggctgattt tgccgatgga
     4201 tctctttgat ataaaacatc attttgccag aagattctgt aaagattttg actaatttat
     4261 ctttttcttt gtaattacgg ctagataaaa tcaagccttt tgattctgca agctggctca
     4321 tattctccct cctaaaaaat aaatctggaa aagcagattc tgccgatcca gatcatttat
     4381 gttgcgatct tccgcagctt ttctcaacac gcggattttt attcagttta tttctatacc
     4441 taatttacaa gaagctcaga atatcagtat tcgtcttttc ggtagccgaa gtcttgcaga
     4501 tagacttttt tgtctcgcca gtctttttga actttgaccc aaagttctag aaagactttg
     4561 tctcctaaaa gattttcgat atcttttctt gctttcgttc ctacttgctt cagcattttt
     4621 ccgcctttgc cgataatgat ccctttttgg ctatctcttt cgacaataat cgtagcctgg
     4681 atatggattt tatcattctc gtttcgcttc atcgaatcaa caacgactgc gacagaatga
     4741 ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga tcaattcaga aacgatgaag
     4801 tattcaggat gatcagtcac ttgatcatct ggaaaatatt gtgggccctc tggcatttca
     4861 ctgactaata catccatcaa acgttcgacg ttgtttcctt ctgtagcaga gattggaatc
     4921 acttgtgcaa aaggcatctg cgttgaataa tcttcgatga ttcccaacaa ctcgtctgga
     4981 tggattttgt cgattttatt gataatcaga taaacaggag aatgcacgtt ttttagacgt
     5041 tcaataataa aatcgtctcc ccgaccgcgt ttttgatcag cactaatcat aaataagacc
     5101 gcgtccactt ctttcaacgc actgtaagct gcttctacca taaaatctcc taaacgatgc
     5161 tttggtttat ggattcctgg tgtatcaata aaaatcaact gagcttctgg tgtagtgtat
     5221 actccttgga ttttatttct agttgtctgt gccttgtcac tcatgatggc aattttttga
     5281 cccacgattc gattcaataa tgtggatttc cctacatttg ggcggccgac gattgcgaca
     5341 aaccctgatt tatgttctgg catatatgtt ctccttttta attaaacaac aaatgatata
     5401 ttttagggac aaataaaatc aacccaacga tgacggtaaa acaagcggtc actaaaactg
     5461 ctccagctgc catatctttg atttttttcc caatcggatg aaaatgaaaa tccgtaaaca
     5521 tgtccacgac attttcaaaa acagtattga tgatctctac tatccatacg agaaaaacag
     5581 ctaaaagcag ccataaccat tctatccgat caagttggaa aatcacgccc ataaacaaag
     5641 cgaggattcc gaacgcaaca tgtttcctca tgttgcgttc ttcctcaaaa acagttttga
     5701 ctccttggac ggcaaattcc acggacatca ggaaatgttt gtttttctct acgtttttat
     5761 cttttaagtc cataggcatc taagatctct ttctgtaaac caaacatttc tttctcatcc
     5821 tctggtgtca tatggtcata cccgttgata tgcaagaatc catgaaccgc taaaaagcca
     5881 agttcccgat catacgaatg gccgtaagct tctgcttgct ctgcagcacg ttcagtagag
     5941 atcatgatgt ctcccagtac acgaggcaat ggattctctc catcttcaaa aatcactggg
     6001 atttcgtctt cgccctcttc ttccaaggca aaactgatga cgtctgtcgg tgcatctttt
     6061 ccacggtaat cacgattgat tacttggatt gctgcattgt ccatgaaagt aacagacatt
     6121 tctgtttctt ctggcaactc taaatatgat gctgcaaatt gcagcaagtc gtcgatttct
     6181 ttcattttct cttctgagac ttggtttgtt tcatccatga aggtaatatc cattatacat
     6241 cgcttctttc tattcttctt gctattcttc ttgttcttct tttatcttct caacttccga
     6301 tttcatttta ggatatttga tccgagaatg gaaggtactg cttaaaccgc tgatcaatga
     6361 tttttctact ttacggattt cttttaacgt caatccactg tcgtccaatt gtccatcgga
     6421 aatacgctct tcaataagat tatggacaaa actttcgatt ttttcgctcg ttggatggtc
     6481 cattgcccgt actgccgctt cacaactgtc agctatattg acgattcctg cttcacggct
     6541 ttgaggtttt ggtccaggat aacggaattg ctcttccgta acttccgggt tgcgttcctt
     6601 tgccttaacg tagaaataac gcatcagcgt ggttccatga tgctgtctac aaatatcgat
     6661 caccatttgc ggcatattat aatcttccaa tattttggca ccatctgtca catgtccaaa
     6721 gataatatgt ttactgtctt ctggcaataa gaaattgtgc ggattttccg ctcctgaagg
     6781 caagttctca acgaaaaagc tggcgtgttt gatcttgccg atatcatgat aatagcaagc
     6841 tactcgagtt agtaaagaac gtccaccgat ttctgctaca gcattagcac ttagattggc
     6901 taccatcatg ctatggtgat acgtacctgg tgcttcttcc agcaactgtt tcaataaagg
     6961 atgattggga ttgctgagtt catttaatac gatcacacta tcatcattga ccatcaattc
     7021 aatatagggg tgcagaccta tcgtcagcaa gaatgaaaac agtgcacctg tgaatccgca
     7081 gactagcgtt aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga taatcaaaat
     7141 gacatccatc atcacaggaa aaataatgac ccacatagca gcggataacc cttgttctga
     7201 gatccgtttg cgtctgacga tcgttgccat gagaccagaa aatacatagg acatcaaaat
     7261 aactgtcaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg
     7321 aaaaatacca gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa caagtggagc
     7381 aaaagccgcg gggtaaaaca gcggaatata agcagcttgt tctgtttgga agagttggaa
     7441 aaatttcatt agtacgacgc ttactgacat tgccgtcacg tagaaaagta cgtattttgt
     7501 tctttctcct gcattttgga attgaagtga attataaatc agtacaccga tttgaagcaa
     7561 aacagctaaa accatggcaa tgagtgggaa aatagaagtc gtttgattcg tcaatcctag
     7621 taattccaat tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa caataatttc
     7681 tccttggaag atcatcactg gttgtacagc ttctcgtgca gattgtttta attcttctgt
     7741 ctttttctca ttcaatacat cattaactac gatgccttga tccactaagt agcgaatcat
     7801 ttgttgctgc tctgttgtga cattcaaata ttgaatctga tcttctgctt cttgtttaaa
     7861 agcttccaat tcgctttcac gaacttgttt actcatctgt tcgtcaatga gcttcaaact
     7921 ttcatcccta actgtgtgaa tttccgtttc agtcatttca aatatcgtcg aataaaaatt
     7981 gttcggcagt ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa aatttttctt
     8041 cagtgtagca atccgctctt ctgtagtagg tttcgggata ttttcatctt ccttcgcctt
     8101 atctaccttg ccattataag atttgttgat ggtatcattc gtcttatcga tcaattcgaa
     8161 taattgcttg atccgattgt tctggtcatc tgccagatct ttttgataag tatattctgg
     8221 agtcactgct tctgctgcca gttttcttct ttgttcggtt tcttccgtgt tctcaatcgt
     8281 tttattcgca cggatactct cttctgctac ttggccttct ctgaaatcaa tggccttttg
     8341 tttgacacta ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca gaaggaaagg
     8401 caatgctgcc tttccgagtt tttcttgtag tttctgcagt gttttattca acatgtgctt
     8461 atcctcctaa gcaaagctta ttcttgatga agatccgcct tttcataagc tttgatgatt
     8521 tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa atcgattttt
     8581 ttgatttgct ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc acgaggcaag
     8641 tctatctgac ttgtatcccc attgacaatc atttttgatt gataaccgag acgggtcaag
     8701 aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca
     8761 tccaatgtcc gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc tcgttccatc
     8821 aatcggttcg tatgatccaa tccaaaaatt tggtataaag catcataaac aggccgcaga
     8881 taaggatcca ctttttcttt cagatccccg ggtagaaagc ctaggttttc ccctgcctcc
     8941 acagcgggcc gcgtcaaaat gattttttgc acttcgcctt tttttagggc agcaattgct
     9001 aatactacgg caagaaacgt tttccctgtt ccggctggcc ctacaccaaa tacgatgtca
     9061 tgtttggcta ccgattcaac gtatttcttt tgaccgctgt tttttacccg tataggtttt
     9121 ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc taatgtacct
     9181 ttttgcccca ttttcaatgc cgtcacaaca tctggcgtac ttacgtggat tcctcgtttg
     9241 atcaattcct gtaatgtccg aatgactgag gcagccaagg aaatctggga ttgctcacca
     9301 atgatctgaa tgatttctcc acgtgtatgg atggttgttt ccgttgtttc ctcgatcagt
     9361 tttatatgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc tgtcaatcta
     9421 atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt cgattcattc
     9481 tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg ttgtaaaaga
     9541 gtcgcttaac catacgtacc aagaaacgtt aaaaaatagc cgtttccatt cgctaaaggt
     9601 ctcgttttga taaaaagaag ctgagaatgt aagttatatg aaagtggaga acaaaatatt
     9661 tcgtttcatt caacaagtat tctctaggta tttttcctgt ttcaaaaaaa gaaaacaaaa
     9721 tagttaaaat attcttgcat ttcaaaaaaa acaggtgtat tattacgtca acaaataaac
     9781 atacagcaaa gcgtcgagga aaacagtagg ctattcaacg atttattccc agtgagtcat
     9841 gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc aatgtcgaaa
     9901 acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat caaaaagcgt
     9961 ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga atctagagcc
    10021 cttttcccaa gtggaaaagg gctttttttt attttaaggt ggtggttttc atggaatctg
    10081 atattagtgg cattactaat tgctgcaacg tttatatcgt caatcaaaat gataattatt
    10141 ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aaacaactat tgatggtcag
    10201 ttcacttatc ttcgggatgt tcttcgggtc aggtaatcta atctttcctg tccacttagg
    10261 tcaaatggct ggcgcaaact gggtcagtgc tggcgtagga tttgctatct caggtgctct
    10321 tttcccatta ctagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt
    10381 agctaaacct gtcggaaaat ggtatgctgc cttgttctta atccttgttc atttgacgat
    10441 cggaccattt ttcggtacac cacggacagc tgctactgct tttgaaatgg ctgctaaacc
    10501 atttttgcct gaaaaatata attcattagg tatgttttta ttctctgctc ttttcttttt
    10561 acttgcttat ttccttactg tccatccaac acggttggtc aaatacgtcg gaaagtatct
    10621 aaactccttg tttcttgcat tattagcaat catttttgtc gtttctttat tccatccaat
    10681 gggaaatcta aatcaatctg ctgcagatac ttatcaaaca aacgctttaa caaatggtgt
    10741 cctagaaggt tataacacgg ttgatgcagt agctttgctt gccttaagtg tcacttttgt
    10801 ccatgcagtc cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa cggcaaaagc
    10861 tggtagcctg agcgttgcgc tagaagtcat gatctatttt ggacttgttc ttctcggtgc
    10921 gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac
    10981 taatttttat ttaggtaact tcggcgctag cttcttagga gtgatggtca cattaggtgt
    11041 cttcactacc gctatgggac tcgttgtttc ttttgcgcaa gatttccata aactgttccc
    11101 taaagtcagt tatatgacat ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa
    11161 tgctggttta gacaatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt
    11221 gtcgcttgcg ttgatcttgc tttccttaac agcaaaattt ttccaaaaga caccatttgt
    11281 ctatcaagta acgatgttgt ttgctgctgt tcctgccgta ttggatatgc tggcaaattc
    11341 gcctgcttta gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt atcatcatca
    11401 cgttcctttt gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg
    11461 cagcctactg ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact
    11521 accagaagaa taaccaaatc aaacacaatt taacataaga aatattaaac aggaaaaaac
    11581 cagctagatg aaagaatcct attctttcgt ctagctattt tttagttctc ttttttacgc
    11641 gcacaaaaaa actgcggaca atgattgtcc gcagtttaaa tgggctctat aatatctagt
    11701 gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac
    11761 cttgaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc
    11821 tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa
    11881 gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg
    11941 acctatcaac cagaggaatg cttccattgt cactatcaaa ataagcaaac gatcattaaa
    12001 t
//
LOCUS       DAZKCE010000091        11812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000091 DAZKCE010000000
VERSION     DAZKCE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.092"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG8KQ_002197"
     CDS             complement(<1..205)
                     /locus_tag="ACG8KQ_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105110.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG8KQ_002198"
     CDS             complement(221..835)
                     /locus_tag="ACG8KQ_002198"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9105111.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG8KQ_002199"
     CDS             complement(982..1665)
                     /locus_tag="ACG8KQ_002199"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9105112.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG8KQ_002200"
     CDS             complement(1774..2232)
                     /locus_tag="ACG8KQ_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9105113.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG8KQ_002201"
     CDS             complement(2316..4079)
                     /locus_tag="ACG8KQ_002201"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9105114.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG8KQ_002202"
     CDS             complement(4300..5760)
                     /locus_tag="ACG8KQ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105115.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8KQ_002203"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8KQ_002203"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9105116.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG8KQ_002204"
     CDS             complement(6917..8344)
                     /locus_tag="ACG8KQ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9105117.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG8KQ_002205"
     CDS             complement(8375..9589)
                     /locus_tag="ACG8KQ_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9105118.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9675..9902)
                     /locus_tag="ACG8KQ_002206"
     CDS             complement(9675..9902)
                     /locus_tag="ACG8KQ_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105119.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9930..10172)
                     /locus_tag="ACG8KQ_002207"
     CDS             complement(9930..10172)
                     /locus_tag="ACG8KQ_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9105120.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10204..10761)
                     /locus_tag="ACG8KQ_002208"
     CDS             complement(10204..10761)
                     /locus_tag="ACG8KQ_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9105121.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10774..10962)
                     /locus_tag="ACG8KQ_002209"
     CDS             complement(10774..10962)
                     /locus_tag="ACG8KQ_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9105122.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="ACG8KQ_002210"
     CDS             complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="ACG8KQ_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9105123.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3465 a         2447 c         1934 g         3966 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9841 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     9901 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
     9961 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
    10021 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
    10081 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
    10141 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
    10201 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
    10261 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
    10321 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
    10381 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
    10441 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
    10501 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
    10561 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
    10621 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
    10681 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
    10741 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
    10801 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
    10861 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
    10921 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
    10981 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
    11041 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
    11101 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
    11161 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
    11221 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
    11281 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
    11341 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
    11401 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
    11461 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
    11521 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
    11581 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
    11641 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
    11701 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
    11761 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt at
//
LOCUS       DAZKCE010000092        11575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000092 DAZKCE010000000
VERSION     DAZKCE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.093"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..755)
                     /locus_tag="ACG8KQ_002211"
     CDS             complement(270..755)
                     /locus_tag="ACG8KQ_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9105124.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    845..1190
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1235..1876
                     /locus_tag="ACG8KQ_002212"
     CDS             1235..1876
                     /locus_tag="ACG8KQ_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9105125.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1895..2530
                     /locus_tag="ACG8KQ_002213"
     CDS             1895..2530
                     /locus_tag="ACG8KQ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9105126.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2548..3363
                     /locus_tag="ACG8KQ_002214"
     CDS             2548..3363
                     /locus_tag="ACG8KQ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9105127.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3682..5118
                     /locus_tag="ACG8KQ_002215"
     CDS             3682..5118
                     /locus_tag="ACG8KQ_002215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9105128.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5307..7754
                     /locus_tag="ACG8KQ_002216"
     CDS             5307..7754
                     /locus_tag="ACG8KQ_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9105129.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7984..9051
                     /gene="rlmN"
                     /locus_tag="ACG8KQ_002217"
     CDS             7984..9051
                     /gene="rlmN"
                     /locus_tag="ACG8KQ_002217"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9105130.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9041..9247
                     /locus_tag="ACG8KQ_002218"
     CDS             9041..9247
                     /locus_tag="ACG8KQ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105131.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9244..10347)
                     /locus_tag="ACG8KQ_002219"
     CDS             complement(9244..10347)
                     /locus_tag="ACG8KQ_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9105132.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10900..11448
                     /gene="aac(6')"
                     /locus_tag="ACG8KQ_002220"
     CDS             10900..11448
                     /gene="aac(6')"
                     /locus_tag="ACG8KQ_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9105133.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3851 a         1951 c         2488 g         3285 t
ORIGIN      
        1 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
       61 atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat
      121 ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt
      181 gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg
      241 aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct
      301 aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata
      361 tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt
      421 tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg
      481 aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct
      541 agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct
      601 tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt
      661 ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt
      721 agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt
      781 agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt
      841 aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg
      901 gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat
      961 agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc
     1021 tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat
     1081 cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc
     1141 tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca
     1201 agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc
     1261 gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc
     1321 attaccatta gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta
     1381 cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt
     1441 tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat
     1501 tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc
     1561 gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt
     1621 gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt
     1681 tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg
     1741 tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat
     1801 catctatctt acattgactg gtattctaac aatcataatg aaacaagttg aaggaaaatt
     1861 aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa
     1921 agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac
     1981 attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg
     2041 cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga
     2101 gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca
     2161 tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga
     2221 agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa
     2281 tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagctattg ccagagcatt
     2341 ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt
     2401 acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat
     2461 tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc
     2521 aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc
     2581 gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg
     2641 actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga
     2701 tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt
     2761 gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa
     2821 ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa
     2881 aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa
     2941 gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg
     3001 tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac
     3061 caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg
     3121 gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa
     3181 ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca
     3241 cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa
     3301 acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat
     3361 taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg
     3421 ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa
     3481 cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc
     3541 attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac
     3601 ataattttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg
     3661 ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt
     3721 ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa
     3781 ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac
     3841 ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa
     3901 caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga
     3961 ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa
     4021 aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat
     4081 gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca
     4141 ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc
     4201 ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga
     4261 atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac
     4321 gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt
     4381 gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat
     4441 tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa
     4501 aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca
     4561 gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga
     4621 gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt
     4681 cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag
     4741 attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg
     4801 gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc
     4861 catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt
     4921 tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg
     4981 ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa
     5041 ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa
     5101 aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt
     5161 gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg
     5221 aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg
     5281 aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga
     5341 aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg
     5401 ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa
     5461 tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt
     5521 tattagccgt attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa
     5581 accgacgctg gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc
     5641 atttatttgg caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt
     5701 tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag
     5761 caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag
     5821 tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa
     5881 atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc
     5941 aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag
     6001 caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa
     6061 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     6121 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     6181 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     6241 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     6301 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     6361 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     6421 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     6481 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     6541 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     6601 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     6661 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     6721 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     6781 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     6841 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     6901 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     6961 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     7021 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     7081 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     7141 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     7201 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     7261 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     7321 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     7381 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     7441 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     7501 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     7561 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     7621 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
     7681 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
     7741 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
     7801 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
     7861 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
     7921 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
     7981 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
     8041 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
     8101 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
     8161 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
     8221 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
     8281 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
     8341 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
     8401 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
     8461 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
     8521 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga
     8581 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
     8641 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
     8701 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
     8761 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
     8821 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
     8881 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
     8941 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
     9001 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
     9061 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
     9121 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
     9181 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
     9241 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
     9301 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
     9361 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
     9421 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
     9481 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
     9541 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
     9601 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
     9661 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
     9721 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
     9781 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
     9841 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
     9901 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
     9961 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    10021 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    10081 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    10141 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    10201 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    10261 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    10321 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    10381 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    10441 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    10501 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
    10561 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
    10621 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
    10681 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
    10741 tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa
    10801 agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa
    10861 aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac
    10921 cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa
    10981 gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg
    11041 gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt
    11101 atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata
    11161 ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt
    11221 tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag
    11281 catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat
    11341 gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg
    11401 gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt
    11461 gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat
    11521 ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt gtacc
//
LOCUS       DAZKCE010000093        11574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000093 DAZKCE010000000
VERSION     DAZKCE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.094"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG8KQ_002221"
     CDS             complement(364..1482)
                     /locus_tag="ACG8KQ_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105134.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG8KQ_002222"
     CDS             1715..1837
                     /locus_tag="ACG8KQ_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105135.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG8KQ_002223"
     CDS             1953..3260
                     /locus_tag="ACG8KQ_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9105136.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG8KQ_002224"
     CDS             3379..4578
                     /locus_tag="ACG8KQ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9105137.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG8KQ_002225"
     CDS             complement(4605..5873)
                     /locus_tag="ACG8KQ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9105138.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG8KQ_002226"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG8KQ_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG8KQ_002227"
     CDS             complement(7090..7593)
                     /locus_tag="ACG8KQ_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9105139.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG8KQ_002228"
     CDS             complement(7716..8480)
                     /locus_tag="ACG8KQ_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9105140.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG8KQ_002229"
     CDS             8588..8863
                     /locus_tag="ACG8KQ_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9105141.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8KQ_002230"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8KQ_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9105142.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACG8KQ_002231"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACG8KQ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11435..>11574
                     /locus_tag="ACG8KQ_002232"
     CDS             11435..>11574
                     /locus_tag="ACG8KQ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105143.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FK"
BASE COUNT         3501 a         2426 c         2040 g         3607 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agca
//
LOCUS       DAZKCE010000094        11484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000094 DAZKCE010000000
VERSION     DAZKCE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.095"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1039
                     /locus_tag="ACG8KQ_002233"
     CDS             194..1039
                     /locus_tag="ACG8KQ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9105144.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1230..2417
                     /locus_tag="ACG8KQ_002234"
     CDS             1230..2417
                     /locus_tag="ACG8KQ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9105145.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2423..2779
                     /locus_tag="ACG8KQ_002235"
     CDS             2423..2779
                     /locus_tag="ACG8KQ_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9105146.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2781..3119
                     /locus_tag="ACG8KQ_002236"
     CDS             2781..3119
                     /locus_tag="ACG8KQ_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9105147.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3195..3451
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3514..4548
                     /locus_tag="ACG8KQ_002237"
     CDS             3514..4548
                     /locus_tag="ACG8KQ_002237"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9105148.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4541..5227
                     /locus_tag="ACG8KQ_002238"
     CDS             4541..5227
                     /locus_tag="ACG8KQ_002238"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9105149.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5251..6099
                     /locus_tag="ACG8KQ_002239"
     CDS             5251..6099
                     /locus_tag="ACG8KQ_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9105150.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6296..7069
                     /gene="sufC"
                     /locus_tag="ACG8KQ_002240"
     CDS             6296..7069
                     /gene="sufC"
                     /locus_tag="ACG8KQ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9105151.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7082..8368
                     /gene="sufD"
                     /locus_tag="ACG8KQ_002241"
     CDS             7082..8368
                     /gene="sufD"
                     /locus_tag="ACG8KQ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9105152.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8365..9600
                     /locus_tag="ACG8KQ_002242"
     CDS             8365..9600
                     /locus_tag="ACG8KQ_002242"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9105153.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9587..10057
                     /gene="sufU"
                     /locus_tag="ACG8KQ_002243"
     CDS             9587..10057
                     /gene="sufU"
                     /locus_tag="ACG8KQ_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9105154.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10062..11453
                     /gene="sufB"
                     /locus_tag="ACG8KQ_002244"
     CDS             10062..11453
                     /gene="sufB"
                     /locus_tag="ACG8KQ_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9105155.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         3747 a         2170 c         2577 g         2990 t
ORIGIN      
        1 agatgaacga atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct
       61 gattgtcttc atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta
      121 tagatggttt tttgtatatt taaatcaaat agtttttata gtaattatca taaaaagtta
      181 tggaagtggt gccatgttta agttattaac gacttttcga gcagtatatg aaacaagaaa
      241 tttttccaga gcagcagaag tactgttttt atctcaacca gcagtctcga atcaaatcaa
      301 acagttggag cgggaattga atatcgatct ttttcaacgt aacggaagaa aagagatgct
      361 tccaactaag caagccgact tattatatga acggacactg gttttgttag aagaatggga
      421 agaaactcag caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc
      481 ttcccacacc tttgctgtct atcttttgcc tcagttgatg aaacatctga tcaaaaggtt
      541 tcctaaagtg cattttgaga tcgaaatcac gaattctcat gaatcattag aacaagtagc
      601 gaaacatgaa tccgattttg gttttatcga aaagccatta gagacttccg gcgtacaaag
      661 atactctttg atggaagacc agctagtcat cgctggggac ccaaaaagca aattgtggct
      721 tgtaagagaa gcaaactctg gtgtttttca ttacacgcag cgttattttg aagagatgaa
      781 tatccaagat aagaagatga tcgtgaaaag taacgatgta attgttgcgt tgctgaagga
      841 aggaatcggt cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc
      901 gttgaatttc catcgatatt tttatttcat ccaacgaacc aatctggttt ctgatcagct
      961 gctagagatt gccgaagaga tccttcgata caatgaaagc acagaaactt ctcacagcga
     1021 ggagaatctc agctggtaga taattgcatg tatcaaaaaa agttctatga tacactaaag
     1081 ataagcagta ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt
     1141 tttcccaaat gtctatggaa agactttcca ttatctaact ggaccgctat aataatcagc
     1201 agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata
     1261 atcgtattga ttatggcgtg attttaccag tctttctgtt atgtttgatt ggtctgctct
     1321 cattatatgt agcacttagc catgatccga acaatccagc agtcttgaaa ggcgtaggga
     1381 tgcaagttgt ctggtattta gttggagcgg cagcaatcgt tgtcatcatg catttgaatt
     1441 ccaaatggat ctggaaactg actccttacc tttatggttt ggggttagtg gtaatgggac
     1501 tcttgctcaa attctatgat cagaatttag ctgcttcaac agggtctaaa aactggtttc
     1561 gattcggtgc gtttactttc cagccagcag agttgatgaa gattgcgtac atcttgatga
     1621 tggcactgat cgtaactaaa cataatacgc aagtaaaaga acggacgatg aaatcggact
     1681 tttggctgat tgggaagctc ttgatcgtga caataccggt tttagcattg atcatggccc
     1741 aagatgactt tggaacgatg cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt
     1801 ctggaatctc ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc gttggagcag
     1861 gaacgatctt cttggttacg actgaaggcg gaagagactt gctttataaa gttggattca
     1921 aatcttatca gtttgcacgt attgattcat ggctggaccc tttccatgat acgtcaggaa
     1981 tgagttatca gccggcccaa ggacttttgg caattggtac cggtggcttg tttggtaaag
     2041 gattcaacgt aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta
     2101 tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat tttcttgtat tttattttaa
     2161 tctatcgtat gattcgggtg tgtttcgata cgaataatga attttacgcc tatatcgcaa
     2221 gcggtttgat catgatgctg ctgttccatg tctttgagaa tattggagca aatatcggat
     2281 tacttccttt aacagggatc ccattgcctt tcattagtca gggaggatcc tcgattttag
     2341 ggaacatgat cggtatcggg ttgatattat cgatgagata ccaaaatgaa gcacctgcaa
     2401 gatcttcaag gaggtaagaa aattgtacac cttttactgg tacccgaaat gttcaacatg
     2461 caaaaaagcc aaagcgtggt tagaccagca tggcgttagt tatcaaaccg ttgatatgat
     2521 caaagaagta ccgaaggcct caacgattga gacttggcta gaaacgagtc aagtaccaat
     2581 ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa cttggactga aagatcaagt
     2641 aggcgagttt acggcttcaa aagcgagcca agtcttatcg acagatggta tgctgatcaa
     2701 gcgaccactg atcttacgag aggaccagct tgttgcaatt ggattcaatg aaaaaattta
     2761 tgaaggagaa ctgatataaa atggcaggca agcatttgaa gaaaaaggac aacctttgga
     2821 tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg
     2881 gagacatcac gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc
     2941 tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc cagcccgcta acgggtacag
     3001 tttcttctgt taatgataaa atcggtgaag atatcaatat cttgaatgat tcagatgaaa
     3061 tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa agaatttgac gaattataaa
     3121 atcagaaaat cacttgaaaa aagttgacac tgattcttcc agttgctata atcataagca
     3181 atcctatatg aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac gtttgaagag
     3241 agccctgttt gctgaaaatg ggtaacgaaa atgagatgga agatggtctt agagcagagt
     3301 aagcgagcgt aagcaaactt actacgggag tgcccgttac agcgcgacag tatacggaaa
     3361 gaagctaagt ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg
     3421 taacacggaa gttcaagctt tcgtcctttt gcttaaggca agagggcgaa ggcttttttg
     3481 tttgaaaaat tataagagaa agatgtgaat ggcatgtcat taatccaact aacgaatgtc
     3541 aaaaagacat tcaatgggaa aaacggaaac atccaagccg tcaatgacgt ttcactaaac
     3601 gttgaaaaag gggatattta tggaatcgtc ggctattctg gcgcaggaaa aagtacatta
     3661 gtccgtctcc tgaatggact tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa
     3721 gatattacaa aattaaaaaa caaagaatta cgtacattcc gaaagaaaat cggtatgatc
     3781 ttccagcatt tcaacctgtt atggtcaaga actgtacttg aaaatatcca gctgccgctg
     3841 gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact gttgcgttta
     3901 gtcgggttag aaggacgcgg acaagcatat ccttcacaac tttccggcgg acagaaacaa
     3961 cgcgtcggta tcgcacgagc attggcaaat gatcctgaga tcctgctttg tgatgaggct
     4021 actagcgcat tggacccgca aacaacagaa gaagtattag acttattgtt agcgatcaat
     4081 aagaaattga atttgaccat cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc
     4141 tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga cgtattgact
     4201 gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga catcgaacca
     4261 gaagctgatt cgacagaact attagctgaa ctcatcaaag aaaatccaag cggccgtttg
     4321 gcaactttga cctttagcga aagaaatgcc aacgagccag tcatttccca agcgattcga
     4381 cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc
     4441 tttggttcat tgactgtctt gatgactggt gaaaccaaag aattagatca agcagaagtg
     4501 ttcatcaaag aacaaggagt aggaattgag gtgatccatc gtggataaaa gcttttcaga
     4561 aacatatcta gattttaaac aaatcaaccc ggaaacgatg aatcaggcta tccttgatac
     4621 actatatatg acattaatct caatggtctt agtgacgatc atcggattag ttttgggatt
     4681 gatcctttac tctattggac gcaaaaacaa accgtatgct cgaatcattt atggaatcgt
     4741 atcgatcatc agtaatattt tccggtcaac gccatttatg atcctgatgg ttttgatcat
     4801 tccatttact aaagcaatcg taggcactat gctaggtgcg caagcagcca ttccagctct
     4861 tgttttatcc gcagcaccat tttatgcccg tctagtagag attgcttttc gtgaagtcag
     4921 ccctggtgta ctggaagcag cagatgcaat gggagcaagt tactgggaaa tcatctggaa
     4981 ggtcttgata cctgaaagta ttcccgcatt gatttctgga ttaacagtta caacgatctc
     5041 gatgatcgga tttactgcga tggccggcgc gatcggcgca ggtggacttg gaggtcttgc
     5101 ttggcaggaa ggctaccagc gtggtaatct aacagtgaca ttcgttgcga ctttaattat
     5161 tttactaatc gtatttatcg ttcaaggaat cggtgatttc ttaactaaga aaacagacaa
     5221 aagataatag gatatagggg gatgtaggat atgaaaaaga aaatttttgg atttgcagca
     5281 gcgcttttgc tgactgtagg attagcagca tgcggtaaca acagcgacag caaagattca
     5341 gcaaataaag cagacgacac gacattgaaa gtcggcgctt caccgacacc gcacgcagag
     5401 attttggaac atgtgaaacc attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa
     5461 ttcgatgact atgtattacc gaaccaatct ttggaagaag gcgacattga tgccaattac
     5521 ttccagcata ttccttattt gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat
     5581 gccggtgctg tccacatcga accaatgggc ttgtactcta aaaaagtcaa agatgtttcc
     5641 gaattaaaag atggtgcaac aatcattact tcaaattctg aatcagactg gggacgcatc
     5701 atcacaattc tacaagatgc tggcttagtc aaagtcaaag atggagtaga tctggaaaca
     5761 gcaacattcg atgatattga agaaaaccct aagaacttga agttcaacca tacgatcgac
     5821 ccatcacttt tagcttctac gtaccaaaat gatgaaggtg acctagttgc aatcaacgca
     5881 aacttcgctt acggcgcagg gttgaatcca ttagaagatg ccgtattgct tgaaaaggac
     5941 aactctcctt acgtgaatat tattgcgact cgtaaaggtg atgaagacag tgacaaaatc
     6001 aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac agacaaatgg
     6061 ggcggctctg ttaaaccagt aaaggctgat gcaaaataag gtatggaaca aaaaagatga
     6121 gaaataagtc tgtaatggct tatttctcat cttttttata cctattctaa tttagaaatc
     6181 aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc attgcgcttc
     6241 attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc
     6301 tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt
     6361 aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa
     6421 atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat
     6481 tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt
     6541 atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg
     6601 tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa
     6661 aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct
     6721 gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt
     6781 agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa
     6841 agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac
     6901 ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg
     6961 ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc
     7021 tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag
     7081 aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa
     7141 aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact
     7201 gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga
     7261 aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc
     7321 tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga
     7381 aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca
     7441 agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt
     7501 agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc
     7561 actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt
     7621 gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga
     7681 acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa
     7741 aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg
     7801 tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca
     7861 tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat
     7921 cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc
     7981 ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac
     8041 ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag
     8101 tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga
     8161 tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat
     8221 gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg
     8281 attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga
     8341 agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct
     8401 atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc
     8461 caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat
     8521 gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga
     8581 gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt
     8641 acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga
     8701 gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg
     8761 gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat
     8821 atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta
     8881 tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac
     8941 ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa
     9001 gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt
     9061 ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc
     9121 gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc
     9181 gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg
     9241 caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg
     9301 ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg
     9361 acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta
     9421 gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac
     9481 cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc
     9541 gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa
     9601 attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg
     9661 aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat
     9721 cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg
     9781 ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat
     9841 agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa
     9901 agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg
     9961 gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac
    10021 cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag
    10081 aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct
    10141 tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg
    10201 aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg
    10261 acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg
    10321 ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac
    10381 cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt
    10441 accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg
    10501 ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg
    10561 acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc
    10621 caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca
    10681 cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg
    10741 aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct
    10801 ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt
    10861 acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg
    10921 gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg
    10981 cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg
    11041 caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa
    11101 aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt
    11161 cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga
    11221 tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa
    11281 aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa
    11341 ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg
    11401 cagtcgaact caatcgattg atcagttatg agatggaagg cagtgttggg taaacctgtt
    11461 ataggagcta gaacccttga tata
//
LOCUS       DAZKCE010000095        11420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000095 DAZKCE010000000
VERSION     DAZKCE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.096"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..176
                     /locus_tag="ACG8KQ_002245"
     CDS             9..176
                     /locus_tag="ACG8KQ_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9105156.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(236..589)
                     /locus_tag="ACG8KQ_002246"
     CDS             complement(236..589)
                     /locus_tag="ACG8KQ_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105157.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1093..1512
                     /locus_tag="ACG8KQ_002247"
     CDS             1093..1512
                     /locus_tag="ACG8KQ_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9105158.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1538..1744
                     /locus_tag="ACG8KQ_002248"
     CDS             1538..1744
                     /locus_tag="ACG8KQ_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105159.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(1972..2145)
                     /locus_tag="ACG8KQ_002249"
     CDS             complement(1972..2145)
                     /locus_tag="ACG8KQ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105160.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2157..2786
                     /locus_tag="ACG8KQ_002250"
     CDS             2157..2786
                     /locus_tag="ACG8KQ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105161.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(2868..4034)
                     /gene="dnaJ"
                     /locus_tag="ACG8KQ_002251"
     CDS             complement(2868..4034)
                     /gene="dnaJ"
                     /locus_tag="ACG8KQ_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9105162.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4185..6014)
                     /gene="dnaK"
                     /locus_tag="ACG8KQ_002252"
     CDS             complement(4185..6014)
                     /gene="dnaK"
                     /locus_tag="ACG8KQ_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9105163.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6065..6628)
                     /gene="grpE"
                     /locus_tag="ACG8KQ_002253"
     CDS             complement(6065..6628)
                     /gene="grpE"
                     /locus_tag="ACG8KQ_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9105164.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6722..7765)
                     /gene="hrcA"
                     /locus_tag="ACG8KQ_002254"
     CDS             complement(6722..7765)
                     /gene="hrcA"
                     /locus_tag="ACG8KQ_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9105165.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(7999..9164)
                     /gene="hemW"
                     /locus_tag="ACG8KQ_002255"
                     /pseudo
     CDS             complement(7999..9164)
                     /gene="hemW"
                     /locus_tag="ACG8KQ_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(9180..9407)
                     /locus_tag="ACG8KQ_002256"
     CDS             complement(9180..9407)
                     /locus_tag="ACG8KQ_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9105166.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9891..10498
                     /locus_tag="ACG8KQ_002257"
                     /pseudo
     CDS             9891..10498
                     /locus_tag="ACG8KQ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            10671..11384
                     /locus_tag="ACG8KQ_002258"
     CDS             10671..11384
                     /locus_tag="ACG8KQ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105167.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVTDN"
BASE COUNT         3305 a         2317 c         1996 g         3802 t
ORIGIN      
        1 agtggatatt gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg
       61 aattaaaaaa attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa
      121 agcagttcaa agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg
      181 agattaatac aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt
      241 cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga ttccattaag gatagttctt
      301 tgtctgtaaa gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt
      361 tattagcagg taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa
      421 gaacttgtat gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa
      481 tttcaatcaa tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa
      541 tggctaaacc aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc
      601 tctatacatt ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg
      661 tttataggtt tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat
      721 ggtagtgcta tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga
      781 ggtcaacgtc gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc
      841 aaaaaaagtc gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat
      901 atagatagaa taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg
      961 ctattcagaa cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca
     1021 ttgggactaa gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa
     1081 gggggtgaga ttatgaggac atcttctttc gagcatatcg tcagattaca gttcaattct
     1141 ttaatgctga ttgtcattaa gaacaaagtg aaaagtcgaa acagacagct tgcaagacat
     1201 tctaaacgtg aagtattgtt ttgtgaaata gccgaaacga aacaagttga acgtggaacg
     1261 attgatacct gtttttatga ttgtgtatca tttaaagtct tgcactttac aatttatgta
     1321 tctgatgaaa cacttggtac agttttacat agactatcag aaaaacagcg tagtgcgatt
     1381 ttattacgtt attttcaagg aatgaacgac agagaaattt cagaactgta ccatgtatca
     1441 cgttctgcta tttgcagtag aagaactaga ggactaagga agttaaaaat gcttttgagc
     1501 gaaaggaagt aaagattatg gctatccctc ttttgaattg atttgcaaag cgtcaagcgg
     1561 agatgaaatg gcagttaaag agattttgaa gttttatgac ggttatattt ctaaattatg
     1621 cttgcgtcct ttttaccact ctgaaagtgg taaaatcatt atgcaggttg atgaagaact
     1681 aaaaggagaa attcatacag acatgatgaa agcaatattg aaatttgaaa tcggagtgaa
     1741 gtaatcaact gtataaaaaa atcgggagat acacagtttc gtgttatctc ccttagtagt
     1801 tttacggtca tatcaaggct cattcaatcg tggtaaattc gtggtaagat gagtgttttt
     1861 ctatacttca aaatgcttga atgtatagtg tttatgcgga taattgaaaa ttagatataa
     1921 aatttaataa aaaccaaaaa tgcacggtcg ccatgaaggg accgtgtatt tttatcttct
     1981 tattttctca cctaatcgta tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg
     2041 ctctacctgt tccatcgcat aagaaagtac catcggttca ttgatgatat caagtacata
     2101 gtcgatacca ccctcactta attgagcttg atttttcttc tccatattat gtagttgtgg
     2161 gacaactttc tgacaatcag caattgtgtc aagaacggat caatccttta ttggagttgc
     2221 tcgaaagggt cttctcttat tacggacagg ctttgctcac tgtacataga caacaaatca
     2281 ttatcctagt caatcggatt tccagagcct cattgctatc attgcttgat aaaatccaga
     2341 caaagtttaa taaaatgtat cagttacagc taaactttgg aattgggtcg ctttgttata
     2401 cagagcaaga gacaccgcaa tctttccttc atgccaaaca agtttgcgaa tggatagctt
     2461 tccatcaatc tgtgaacgag atccgctttt ttgaagattt agacctcggg atcgttttac
     2521 cagcaatccc gtcagatcaa cgaacattat atgtaaaacg tattttgaaa agcctgacag
     2581 aagaagaagt acatttgttc aagaagactt tggcatgctt cagtaaaaat aacggaagca
     2641 tcaaaaattg ctcagaagaa ttatttattc ataaaaacac cttgcaatat cgattgaaca
     2701 agttccattc tctcactggt tataccccta gaaactatga cgattatcat attctaaagc
     2761 tggctttttt gttggtacaa acatgacaac tacaaaaaaa ccaggcgaac atatgattgt
     2821 tcgtctggtt tttaattatt aatgacttag cgtttttact agaaaaacta cttccgcttt
     2881 ttcccgccaa aagcatcttt aaacttatcg aagaaaccgt cttcttgctg ttcttcaact
     2941 ttgatgccgc cagcttcagc gaatgcacgt aatgcttctc tttgttgttc atttagattt
     3001 ttcggtgtga tcagttttac tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc
     3061 ggagcgcctt ttccacgtaa acggaagttc gtacctgtct gtgttccagc agggatcttc
     3121 aatttcacat ctccatggac agttggtact tggacttcat cccccaaagc tgcttgtaca
     3181 aaacttaaag gcaattcata gtaaatttca gaaccttcac gatcaaagat atcactttct
     3241 tctacacgga agaccacata caaatcacca aatggtccgc cgttttctcc agcttctcct
     3301 tgaccagcta aacgcatttg ttgtccttct tctacaccag ctggtacatt cactttaact
     3361 gaatgtgctt tcttctcatg tcctgagcca tgacaggttg gacaaggttc tttgatttct
     3421 ttccctgtcc catgacaaac atcacaagtt tgacggctca tcactcgtcc aagtggtgtt
     3481 tggcgttcta cattaatcgt acctgcacca tgacatttat gacaagtagt tggttgtgtg
     3541 cctggttttg caccgttccc accacaagta tgacagattt cttcccgatt atacttgatt
     3601 tctttttcta caccaaaaat cgcttcttca aaagacaagt cgaccgtata ttgcaaatca
     3661 gcaccttgac gaggcgcatt tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg
     3721 aagatatctt caaaaccacc gaagccgcca ccagagaagc cgccgaatcc accaaatccg
     3781 ccaccagctc caccagcacc gtaatttgga tctgtgcctg catgcccgta ttggtcatat
     3841 gcagcgcgtt tttgcgggtc acttaaaatc tcatatgctt ctgatacttc tttgaatttt
     3901 tcttccgcat caggttcttt attgatgtca ggatggtatt ttttcgaaag cttacgataa
     3961 gctttcttta tttcatcttc tgatgctcct tttgagagcc ctaagacttc ataataatca
     4021 cgttttgttg ccataggctt tcctcctatt ggttctcatc attttcacgt cgcttgttat
     4081 catatcatat tttgtttgaa acaaaaaccg atacttcaaa ctatttttca aaagaaaaag
     4141 ccaaagtagc aactttggcc ttctctcgaa attcagtgaa ctgattattt atcgtcgcca
     4201 tcgacttctt caaaatcagc atcaacgaca tcatctgctc cgccttgagc tgcttctggg
     4261 ttttcttgag cttgttgttg tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg
     4321 atttcgttca atgcatcgcg tttagttttc atttcttcga tgttgtttgc ttcaacagcg
     4381 gctttcaatt catcgcgagc tgtttctgct tttttcactt cttcttcgtc cactttgcct
     4441 tctaattctt tcaatgtttt gtccacagag aatagcaatg catctacatc attacgtagg
     4501 tcaacttcct ctttacgagc tttatctgct tctgcatttg cttctgcatc ttttaccata
     4561 cgttcgattt cttcgtctgt tagacctgaa gaagatttga tcgtgatttt ttgttctttt
     4621 tgtgtaccta gatctttcgc agatacattt acaatcccgt ttttatcaat atcgaatgtt
     4681 acttcgattt gtggaatacc acgaggagct gctggaatat ctgtcaattg gaatcttcct
     4741 agagttttgt tgtcagcagc cattgggcgt tcaccttgta atacatggat gtctactgct
     4801 ggttgattat ctgccgcagt tgagaatact tgtgatttac ttgttggaat cgttgtattg
     4861 cggtcaatca atttagtgaa cactccaccc attgtttcga tacctaatga caatggtgta
     4921 acgtcaagta aaacaacatc tttcacatca ccagtgatca cgccaccttg gatagcagca
     4981 cccatagcaa ctacttcatc agggttaact gatttgtttg gttcttttcc tgtttcttta
     5041 cgtactgctt ctacaactgc agggatacga gtagatccac cgactaaaat aacttcgtcg
     5101 atttctgatt gtgataaacc tgcatctttt agcgcttgac gtactggtac ttttgtacgt
     5161 tctactaagt cagctgttaa ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc
     5221 aatggtccag attctcctgc tgtgataaat ggcaagctga tttgtgtgct tgatacgcca
     5281 gataaatctt ttttcgcttt ttcagcagcg tctttcaagc gttgtaaagc cattttgtct
     5341 tgagacaagt caatgctgtt ttcttttttg aattctgcta ccatgtagtc gatgattttg
     5401 ttatcaaagt cgtccccacc tagatggttg tcgccagctg ttgacaatac atcaaagaca
     5461 ccgtctccta gttcaaggat agatacgtca aaagtaccgc caccaaggtc gaatactaag
     5521 attttttcgt cgcgatctgt tttatctaaa ccataggcaa gagcagctgc agttggttca
     5581 ttgacgatac gttctacttc tagaccagcg atcttaccag catcttttgt tgcttgacgt
     5641 tgtgcatcat taaagtaagc tggcacagtg ataactgctt tttcgacttt ttcacctaaa
     5701 tagtcttctg cgaatccttt gatatattga aggatcattg ctgagatttc ttgtggtgta
     5761 tatgatttac cttctacatc tactttgtaa cctgcttcac cgatatggcg tttgattgat
     5821 gaaatcgtat ttgggtttgt cacagcttga cgttttgcaa cttcccctac ttggatttct
     5881 ccatttttga atgatacaac agaaggtgtt gtacggtttc cttctgggtt agcgatgatt
     5941 tttgcttcgt ttccttccaa tactgctact gcagagttag tcgtacctaa gtcaataccg
     6001 ataattttac tcataattaa cgtctccttc tttaattgaa agtgtaagtt ttatatccgc
     6061 acaattattg tgcaacaatg accatggttg gtcttaacac gcgatcatgt aatttgtagc
     6121 cttcttgtag tacttctaca atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt
     6181 gtactgcttg atgaagatta ggatcaaagg cttctccttt agcagggatt ttttcaattc
     6241 cttcttcttc cagtgcgtta cgcaaacttt ccaagaccat ttccacgcct tttttcaaac
     6301 ctgccccttg atcatctgac acttctgtgg ctaatgcacg ttccaggttg tcaattgatg
     6361 gaagtaattt tttggctaaa tcttgtgaac ggtatttttg taattgctca cgctcatttt
     6421 ttcctcgatt tgccatattt gcgatttcag cacgagcccg caagtattta tcttccattt
     6481 cttctaactc agcttttaat ttcgctgttt cgaattcttc cgcttcagct tctgaaatac
     6541 ccgaatcttc tgcagcttcg acatcgattt ccggttctgg ctgagcatct gtcatttctt
     6601 tatctagttc ttcttgattt tctttcacga tacagtcttc ctttcattat tcagtcagct
     6661 gtttgattgc tcttactaag aaagagagaa cgatcagctg caaatagctt cgttattcac
     6721 attacgagtt tgacaagtca agcgaacggt agtaatcagc aagtttagct gctagttctc
     6781 ttcgataaac atcgactaga ccgaacatcc gagaataagg catactagta ggccctaaca
     6841 aggctattgt acctcttcca tgaccatcta tctcgtaact tgcttgaatc atactcatat
     6901 tctgcaataa atcattgcct aattcagcac caatcttgat atgaatattg ctatccatcg
     6961 gagtgatcat ttgtgtaagt ccgtctgagt ctttcataaa agaaaacatc gatttgaact
     7021 gatcaagatc ttgacggggc tcataatcca agagattcat ttgtccactg acataaacct
     7081 tatcttcaaa aacatgacca agaacggtgt caaataactg agacatccct tctgtcgttt
     7141 ggaaatattt atgaagaatc atcggaatct ccgtacgaag tttatggtac accgtcatca
     7201 gaggatctcc gactagacgg tcgtttacaa tctgtaccat tttttctagt tcctgacttc
     7261 ccatatattc gggaagagtg aacacttggc tttctacatt ccccatatct gttacgacga
     7321 tagcaataat ctgtcgatta ttcaagggta cgatacgaaa gccagtcagt cggcggtctt
     7381 ttatttcagg tcccaatgaa aaagtcgtat agcttgtcag ctcagataat atctcagcag
     7441 attgcttaat aatctcattg atttcatgaa attctctgcc tagagactga cgaatcagca
     7501 gcaaatcatc ttctgctacg cgagcaggtt ttaacaaatg atccacgtaa taacgatagc
     7561 ctaaagcaga tggaatccgc ccagaagaag agtgagtctt caacagcaat ccttcatctt
     7621 ctaatgcttt catatcattg cgaatcgttg ctgagctggc cttgactccc tcagacatca
     7681 atgttttgga accaacgggc acgccactac tcgtatagtg ttggatgatc agacgtaaaa
     7741 tgtcgttttg tcgctttgtt aacatcttcc tcacctctca ttagcactca tatattctaa
     7801 gtgctaatcc acttataaat atagcaagaa gcccttctcc tgtcaagaga aagactccat
     7861 tttttagcac tctaaacaca agagtgctaa ttctaaataa ttgatgtaaa tccagttata
     7921 tcaacgtttc aatagtcctt tattgtattt ttgaactttt tttatttaaa ttcagataaa
     7981 aaacaaatat tctttaaact atttttcaaa caaaaagcgc tcaaacacat tgttccccaa
     8041 aaaggtccct cttggtgtca aataaacacg gtcttcttca ttaacaagta acccttcttg
     8101 ctctaattcc cttagcacat caccgtacac cgattcaatt gttttttgat agcgatcata
     8161 gaaatgtttt ttagtaatcc ctgttttttt cttagtccta agaacatttc ttcttctatt
     8221 tgattttttc gtgtcaattt ttcttcttcc aataccgggc gttcatttgc tcgtaatggg
     8281 cgtaaatagt gttggattgg tcctttgttg cggtaacgaa tatctccaag ataaccgcta
     8341 gcaccagctc caaacccaaa atagtggtca ttgttccagt acatgaggtt atgttgagat
     8401 tcatgtccgg agagcccaaa gttacttatt tcatattggt gacgtccatg cttttccatt
     8461 gcttcaatcg tctcttcaaa cattctggtt tccgtttcct gatcaggcag ttcgagtctt
     8521 ccttgtcgta cccagttcat gaacattgtt ttgttttcga ggatcaatga gtacaaagaa
     8581 tagtgaggca gatccaaagc caaagctttt tccaaagtat ccagatatcc ttctagtgtt
     8641 tgtcccggta atgcataaat caaatcgatg cttacattgg taaaattttc tgcttctaaa
     8701 aattgcatag tttgatacac atcttctgcg gtgtgtttac gtccaatttt tttcaataag
     8761 cgattatcaa atgtctgcac tcccatggat aaacgattga cgccatagtt tttcattact
     8821 tgaagttttt cacgggtcaa atcacctgga ttcgcttcta cagtaaactc attaccattt
     8881 tgaaaaggca ggatctctct agcacctgat aacagtcgat caagttgttt agcagataaa
     8941 gaagttggtg tcccgccccc tacgtaaagt gtttctaaat tcgctgtcgg atgactttct
     9001 agcattattt tcatttcatt cagcagcatt tccacatact catctactgg ctgcccttct
     9061 aaaaaaactt tattaaaatc acagtaatag cagatatgct cgcaaaatgg gatatgaatg
     9121 tacgcagaaa ccttgttttc tgcgggattt attgtttcta tcattgaaat tctttctcct
     9181 tacgtcgaaa agtcgtgcct gttttcgacc ttcttaaaat cgcgttcaag caccaactca
     9241 ttgaaataag ccgcatattt agaatcaagg aaacctagct ctaaaaatgc ttgcttattg
     9301 tcatcggagc tacacggtgc ttttacagcc tctcgataaa cggtaatcgc aaagctgacc
     9361 ttcggcatta agtcaaaatc tgcaaaaata tgaaaagcta ttttcacaga ttcctcctta
     9421 agtgctcaga ccacaacgct ttgcttacaa cctctcatat acgcatacta aacgtatttg
     9481 gcgaacaaat ccactaaaaa atccaagaat cgacttcttg gatgattttt tctttcatta
     9541 aactggttac ctagttatat atctctaggg ttgttttcat gtctgagtgc ccgatatgat
     9601 ttatttttcg tttctataat tactttttgc cttccagttt cttttaatat tgtactcaag
     9661 caagtgggaa ttggttcttt tgtcatccat gtatgcaaaa tgttataatg ctggtaaaca
     9721 aaaatgatta cctgtcattt ttatttagtt cgtatttaat taagttgact atctgtatac
     9781 tctgtctaaa acttaagaat tgttgttttc gctgtcgcta cccaatttta atgaatgtct
     9841 gtaggttaaa actgttgtcc ttgttataga tagtaaagga gagcttgaaa atgaaaactg
     9901 cgattacgtt tctattagag gaatatgccg attgggaagg tgcttatatt actagcacac
     9961 taaaccaaaa tgagaaatgg caaaacaaaa ctgcttctat ccaatcagag gtcacttcca
    10021 ttggaggatt aaaaacaaaa atcgattact taatcgaaca aatacctgaa aaagttgacc
    10081 tttttattat gatcggtggc aattcatggg acataaaaaa tcatcattta tatcaaataa
    10141 tcgataaatt gttaaaaaca gacgtaccag tagctgctat ttgtggggca gttgattatt
    10201 tagctcagag cggattattg caaggttaca aacacaccgg aaatgcgcaa tttccttggt
    10261 tagaatcgga aaattatcag aataaagaag attttataga acaacaagct gtgaaagatc
    10321 gtaatttgat tactgcgaat ggaacagctc ctatagaatt cacagacttg attcttcaag
    10381 cagtccattt tgaaacagaa gaaataagag aaaaaacgat tcgtctatac aaacttggat
    10441 tctatgatta ctataaaaag tacggagatc ctttctccta atcattctct tcaattaaat
    10501 actccttatc aatagtgtga caagtaattt tactgatgat tatttgtcac actttttctg
    10561 acatttatca aagaagatac actctattta tattcataga caaggtttta cggattcaaa
    10621 aattcaggat tatgctatac tggtattaaa aaaataatga ggtattttat atgaaaaagt
    10681 taggttttat tttattgttg gtctctttat tccttgtagg atgtacgtct cctagtaaag
    10741 aaaatagtga tccatctgcg accacagaca aaagttcaat cagacaaacc agttcagctg
    10801 aaaaatcaag tactcagaaa tcagcttcta ctgaaacgaa tacatcttca acaagcagta
    10861 aagtctcaga gcccaatact tcttctacat ctagtagtac agttgagaca aatatttctt
    10921 cttcaacaat agacacaacc tcctcacagt cttctcagtc aatagatccg ttagcaggat
    10981 attctgatct acaaattgag tacgcacgtg tttggctagc tattagaggt aatactttta
    11041 gagatacatc gggtgatttc gaattacatg ttattcatta tcctgcagga acacctatcg
    11101 ccccttatga tgcagggagt gcagtctatc cgaaagatgt ggttatgctc actgggaaat
    11161 acagttacca gggagtttta gtgtattcaa gtaaccatga cgggacaatc actagttatc
    11221 cagttccttc acattggcaa atgcctgcag atcaaacatc ttatccagcg ttcattagag
    11281 aaacaactca agaaatcatt gataatgcat ctgtagtagc tattcctaca ggaaatccca
    11341 atgacgtgaa acagttaatc gatgtgacag ttacagataa ttagactttc taatatgtta
    11401 tacaaaaaga agctgacagc
//
LOCUS       DAZKCE010000096        11358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000096 DAZKCE010000000
VERSION     DAZKCE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.097"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..367
                     /locus_tag="ACG8KQ_002259"
     CDS             161..367
                     /locus_tag="ACG8KQ_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9105168.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(569..1570)
                     /locus_tag="ACG8KQ_002260"
     CDS             complement(569..1570)
                     /locus_tag="ACG8KQ_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9105169.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1575..3488)
                     /locus_tag="ACG8KQ_002261"
     CDS             complement(1575..3488)
                     /locus_tag="ACG8KQ_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9105170.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3656..4162)
                     /locus_tag="ACG8KQ_002262"
     CDS             complement(3656..4162)
                     /locus_tag="ACG8KQ_002262"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9105171.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4322..4762
                     /locus_tag="ACG8KQ_002263"
     CDS             4322..4762
                     /locus_tag="ACG8KQ_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9105172.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4788..5903)
                     /locus_tag="ACG8KQ_002264"
     CDS             complement(4788..5903)
                     /locus_tag="ACG8KQ_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105173.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5948..6304)
                     /locus_tag="ACG8KQ_002265"
     CDS             complement(5948..6304)
                     /locus_tag="ACG8KQ_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105174.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6602..7576)
                     /gene="sgrA"
                     /locus_tag="ACG8KQ_002266"
     CDS             complement(6602..7576)
                     /gene="sgrA"
                     /locus_tag="ACG8KQ_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP9105175.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7772..8611)
                     /locus_tag="ACG8KQ_002267"
     CDS             complement(7772..8611)
                     /locus_tag="ACG8KQ_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9105176.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8796..9683
                     /locus_tag="ACG8KQ_002268"
     CDS             8796..9683
                     /locus_tag="ACG8KQ_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9105177.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10277..10972)
                     /locus_tag="ACG8KQ_002269"
     CDS             complement(10277..10972)
                     /locus_tag="ACG8KQ_002269"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9105178.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10956..11354)
                     /locus_tag="ACG8KQ_002270"
     CDS             complement(10956..11354)
                     /locus_tag="ACG8KQ_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9105179.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3386 a         2280 c         1698 g         3994 t
ORIGIN      
        1 ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa
       61 gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta
      121 atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc
      181 tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta
      241 tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt
      301 gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca
      361 aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa
      421 atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc
      481 ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag
      541 attacaacct cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat
      601 atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat
      661 ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta
      721 cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg
      781 tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt
      841 ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac
      901 ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct
      961 gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg
     1021 ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg
     1081 accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga
     1141 taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt
     1201 gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta
     1261 catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg
     1321 tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct ttttttatat
     1381 cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac
     1441 ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca
     1501 aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact attcgcttga
     1561 accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca
     1621 gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac
     1681 attctttttt ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta
     1741 tcatcacttg attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc
     1801 ggatcttgct tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt
     1861 atcgtctcat taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg
     1921 aatgtctttt gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc
     1981 tggtctttga gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg
     2041 aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt
     2101 gcctttccat gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca
     2161 tataattttt gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat
     2221 ttaggcaatg aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc
     2281 tcctgatcaa aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc
     2341 ccaatgatgt ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg
     2401 tacgtaaaac gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg
     2461 acattgacag gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca
     2521 ccataaagta aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg
     2581 acgtcatctg actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct
     2641 ctggaatcaa caaataataa attatacccg gccttataaa aatcattgta ttcttctgta
     2701 acgatcccgt tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa
     2761 ccttgatcga cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca
     2821 gtttgatgaa gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca
     2881 tcatctgtac ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac
     2941 aatagataag tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca
     3001 gcaagctcag tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta
     3061 agtatttctg tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca
     3121 cctttaccga tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag
     3181 gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta
     3241 gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt
     3301 tctgtttgtg aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct
     3361 acctcccgag attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg
     3421 cttgtactta cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag
     3481 ctattcactc ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac
     3541 aaaaaataaa tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat
     3601 aaagcagaat ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt
     3661 gctccaaaaa tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct
     3721 ttgctttcat aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt
     3781 atcctaatcc atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac
     3841 ataattcgat cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa
     3901 atgaattcgc atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca
     3961 atagctccca tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa
     4021 ctggtttccc aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt
     4081 ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta
     4141 ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat
     4201 ttcttatcta gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg
     4261 gcacttttcg actatttctt catgttatta tttcataaat acttagctat tggaggaatg
     4321 tatgatgatt aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg
     4381 gcttatcgtc catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa
     4441 aaatgaattt atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac
     4501 tcattctatt agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact
     4561 atatattgag attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc
     4621 tgcaaaactt ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa
     4681 aagaaagcaa gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa
     4741 taatcttttt gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa
     4801 tccaattttt ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc
     4861 agacgcgccc aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt
     4921 tgatcatatg cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca
     4981 gcggatgcac tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat
     5041 aattcctgtg ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt
     5101 ataaaaatat ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa
     5161 taagtattaa gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga
     5221 tacatcactg gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc
     5281 accttctttt ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat
     5341 agataatttt tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca
     5401 atagacgatt caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct
     5461 tcttccaaat aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga
     5521 gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta
     5581 aactcttctt ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta
     5641 atcgagtggc cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt
     5701 ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta
     5761 ttttttatac ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag
     5821 tgaaaaaagt atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg
     5881 caagataaat gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta
     5941 cgcaaactta tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa
     6001 gtgaatccag taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca
     6061 gtaaaaactg agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt
     6121 tatattctgg atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag
     6181 ccagggtgta gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt
     6241 cataacaata gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa
     6301 acaaagtaca tactcctttt tatctattat ttagacttat tccgacagct acatgaataa
     6361 cgtacaaata gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc
     6421 aattattctt gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt
     6481 tagtaaacaa aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga
     6541 atgaaatatt cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc
     6601 tttatttcac tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta
     6661 acacacccat ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc
     6721 tttttctatt atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat
     6781 caggctgttt tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg
     6841 gtatgatagg taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg
     6901 tgcttcctgt gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct
     6961 ccgtactgct ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac
     7021 tgctttcgct gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt
     7081 caccgctcgt gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc
     7141 caacaactgt ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa
     7201 aaacaatcaa atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa
     7261 tcattattgt atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa
     7321 taactttttc tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac
     7381 tagcattcac gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat
     7441 aaacaatccg atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag
     7501 aagtactgat tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg
     7561 tcgctgtttt cttcatttct atcactctcc atataattac cttctatttg ataaaggaat
     7621 tatataaaat gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt
     7681 cgtgtattcg cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca
     7741 agaccgatat cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca
     7801 tccgctttgt tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg
     7861 tagttgatat agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt
     7921 ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag
     7981 ctccttgtgt ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac
     8041 tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg
     8101 tcatgttagg attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta
     8161 ttaacggacc aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg
     8221 atgcccccac tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc
     8281 ctgaatctaa ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc
     8341 caattccaac aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac
     8401 cataatcttc tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa
     8461 cagctaaatg tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca
     8521 tatccgcaag tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa
     8581 cagatccaat caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa
     8641 caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat
     8701 agatttttac gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct
     8761 aattcttctg ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac
     8821 tactaattat gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca
     8881 atgaaacgat tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc
     8941 caaaagttaa atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg
     9001 atgatgtata cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa
     9061 tcaaaaaact cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga
     9121 catataagaa actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa
     9181 aattaacaga taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac
     9241 agatcattct attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag
     9301 gagaaaattt ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg
     9361 atggaaaaat gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag
     9421 catttaaact aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt
     9481 atcagcaaag ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag
     9541 acaaatacaa atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat
     9601 tcatggataa aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt
     9661 atgtaaaaaa tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga
     9721 gaagtgattt ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca
     9781 tcggggcctt tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat
     9841 ataatttaaa aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct
     9901 ttcatgcttc cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca
     9961 aactcgactg aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc
    10021 ttattctgtt atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt
    10081 ttatgaaatt tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag
    10141 ggaatacatc gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa
    10201 ttaatgaata ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt
    10261 ttatggagaa attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg
    10321 cttggaaagt ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca
    10381 tctctaatac ttgatctagt agctctttac ctaacgttat cgcatgagct ccagatcgaa
    10441 tggctgtagt gacttgttca caatttttga aactagctgc taatattttt gttggtcttt
    10501 gcgtacgttc gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat
    10561 tttgcatcct gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc
    10621 cttgtaattc tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt
    10681 tgatcgcttg aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt
    10741 cttctatgat aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa
    10801 tatggatagg aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg
    10861 acgctccttc ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact
    10921 gttgacaaga ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt
    10981 cccccattct gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt
    11041 tccttatctt ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt
    11101 atcgtgatat gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt
    11161 acatacattt gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga
    11221 ttcaattcaa tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa
    11281 atcattattt tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt
    11341 tcaaagatcg tcatctct
//
LOCUS       DAZKCE010000097        11349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000097 DAZKCE010000000
VERSION     DAZKCE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.098"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG8KQ_002271"
     CDS             complement(46..1557)
                     /locus_tag="ACG8KQ_002271"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9105180.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8KQ_002272"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8KQ_002272"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9105181.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG8KQ_002273"
     CDS             complement(3384..4460)
                     /locus_tag="ACG8KQ_002273"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9105182.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG8KQ_002274"
     CDS             4656..6401
                     /locus_tag="ACG8KQ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9105183.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG8KQ_002275"
     CDS             complement(6681..7118)
                     /locus_tag="ACG8KQ_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9105184.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG8KQ_002276"
     CDS             complement(7102..8475)
                     /locus_tag="ACG8KQ_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105185.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG8KQ_002277"
     CDS             complement(8797..9285)
                     /locus_tag="ACG8KQ_002277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9105186.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG8KQ_002278"
     CDS             complement(9307..9909)
                     /locus_tag="ACG8KQ_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9105187.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(9978..11317)
                     /locus_tag="ACG8KQ_002279"
     CDS             complement(join(9978..10876,10876..11317))
                     /locus_tag="ACG8KQ_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP9105188.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         3304 a         2364 c         1979 g         3702 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc cttatgaact
     9961 gaaccccaaa agttgaacta tttaatggac tgtttccgat attctactgg agatagtcca
    10021 tttaacttta gttttatcct tttgttatta taccacctaa tatactcatt taatttactt
    10081 tgaaatatct caattgatcg gaatttttct cgataaaaaa actctgattt aagcacacca
    10141 aaaaaatttt ctattacaga attatctaag caatttcctt ttctagacat gctttgaata
    10201 atgttattct cttttaattt tttttgatat tgtggcatct gatactgcca tccttgatct
    10261 gaatgtagaa tcagtgaacc ctcagttccc ttttttttaa ttgcttgttg aagcatttct
    10321 tcaatcagtt tatatgttgg actagttgat atactataac taattatttc tccgttaaat
    10381 aaatcaagta taggagatag atagattttt cttcctttta tcttgaattc agtgacgtct
    10441 gtcacccatt ttttattggg tgtatctact gaaaaatttc gttttaacac attcttggca
    10501 atttttccta ctgttccttt ataagattta tatcgcttta ttcggatttg acaggtaagt
    10561 cccatttggg acattaattt tctaactgtt ttatgattga ttgtatatcc tttcatcttt
    10621 aacgctaaag tgactctacg ataaccataa gagtttcttg attcttttac aatcgctgta
    10681 atttcttgct ttatcttgct atatttatct ggcttatcta atttttttac ccagtaataa
    10741 taagttgact tcgctaattg tgcaattgaa agtaatagat tcaatttaaa ttcttttttg
    10801 agctgaagga ttgttttaac cttgatttct tcttgctcaa atcttgttct tgaatcaagg
    10861 tttctaactt ttttaaatat gcgttctctg ctcttaaacg aataacttct tcttcaagag
    10921 actcatcttt aagtttttta ggaatgttta gcttggaatt catactaatt tttctgcccc
    10981 ttttttggct ctcaagtgaa gaagcaccgc cttcttcata ttgctctatc catttactta
    11041 gagttctatt tgaaccgata ttaaattttt tagcagtttc ttggatagaa agaccatttg
    11101 tttccatata ttctataaca tcaagtttaa attttgtagt gtagcttttg ccacctccaa
    11161 ccaagccttc ccaaccatga tagttataaa tccttaccca atgtctaacc agtgtacgat
    11221 ttatttgata tttatgtgca agatatttgt agccgccttc gttatttaaa tagtctgaaa
    11281 ctactttttt cttaaaaaca aatgtatatt tccgcaaaaa aagcacccct tttaattaga
    11341 tttctagtc
//
LOCUS       DAZKCE010000098        11264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000098 DAZKCE010000000
VERSION     DAZKCE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.099"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1485
                     /gene="lysA"
                     /locus_tag="ACG8KQ_002280"
     CDS             226..1485
                     /gene="lysA"
                     /locus_tag="ACG8KQ_002280"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9105189.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1472..2272
                     /gene="dapF"
                     /locus_tag="ACG8KQ_002281"
     CDS             1472..2272
                     /gene="dapF"
                     /locus_tag="ACG8KQ_002281"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9105190.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2275..3156
                     /gene="dapA"
                     /locus_tag="ACG8KQ_002282"
     CDS             2275..3156
                     /gene="dapA"
                     /locus_tag="ACG8KQ_002282"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9105191.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3153..3869
                     /gene="dapB"
                     /locus_tag="ACG8KQ_002283"
     CDS             3153..3869
                     /gene="dapB"
                     /locus_tag="ACG8KQ_002283"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9105192.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3875..4570
                     /gene="dapD"
                     /locus_tag="ACG8KQ_002284"
     CDS             3875..4570
                     /gene="dapD"
                     /locus_tag="ACG8KQ_002284"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9105193.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4586..5587
                     /locus_tag="ACG8KQ_002285"
     CDS             4586..5587
                     /locus_tag="ACG8KQ_002285"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9105194.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5584..6945
                     /locus_tag="ACG8KQ_002286"
     CDS             5584..6945
                     /locus_tag="ACG8KQ_002286"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9105195.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6905..8056
                     /locus_tag="ACG8KQ_002287"
     CDS             6905..8056
                     /locus_tag="ACG8KQ_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9105196.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8031..9200
                     /locus_tag="ACG8KQ_002288"
     CDS             8031..9200
                     /locus_tag="ACG8KQ_002288"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9105197.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9375..10754
                     /locus_tag="ACG8KQ_002289"
     CDS             9375..10754
                     /locus_tag="ACG8KQ_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9105198.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11035..11264
                     /locus_tag="ACG8KQ_002290"
                     /pseudo
     CDS             11035..11264
                     /locus_tag="ACG8KQ_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3749 a         1958 c         2508 g         3049 t
ORIGIN      
        1 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
       61 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
      121 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
      181 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
      241 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
      301 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
      361 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
      421 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
      481 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
      541 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
      601 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg
      661 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
      721 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
      781 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
      841 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
      901 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
      961 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     1021 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     1081 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     1141 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     1201 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     1261 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     1321 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     1381 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     1441 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     1501 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     1561 tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt
     1621 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     1681 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     1741 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     1801 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     1861 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     1921 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     1981 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     2041 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     2101 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     2161 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     2221 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     2281 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     2341 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     2401 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     2461 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     2521 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     2581 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     2641 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     2701 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     2761 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     2821 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     2881 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     2941 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     3001 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     3061 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     3121 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     3181 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     3241 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     3301 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     3361 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     3421 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     3481 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     3541 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     3601 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     3661 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     3721 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     3781 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     3841 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     3901 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     3961 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     4021 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     4081 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     4141 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     4201 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     4261 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     4321 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     4381 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     4441 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     4501 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     4561 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     4621 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     4681 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     4741 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     4801 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     4861 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     4921 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     4981 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     5041 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     5101 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     5161 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg
     5221 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
     5281 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
     5341 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
     5401 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
     5461 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
     5521 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
     5581 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
     5641 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
     5701 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
     5761 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
     5821 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
     5881 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
     5941 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
     6001 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
     6061 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
     6121 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
     6181 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
     6241 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
     6301 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
     6361 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg
     6421 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
     6481 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
     6541 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
     6601 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
     6661 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
     6721 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
     6781 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
     6841 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
     6901 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
     6961 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga
     7021 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
     7081 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
     7141 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
     7201 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
     7261 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
     7321 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
     7381 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
     7441 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
     7501 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
     7561 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
     7621 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
     7681 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
     7741 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
     7801 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
     7861 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
     7921 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
     7981 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
     8041 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
     8101 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
     8161 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
     8221 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
     8281 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
     8341 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
     8401 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
     8461 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
     8521 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
     8581 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
     8641 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
     8701 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
     8761 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
     8821 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
     8881 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
     8941 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
     9001 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
     9061 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
     9121 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
     9181 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
     9241 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
     9301 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
     9361 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
     9421 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
     9481 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
     9541 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
     9601 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
     9661 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
     9721 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
     9781 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
     9841 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
     9901 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
     9961 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    10021 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    10081 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    10141 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    10201 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    10261 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    10321 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    10381 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    10441 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    10501 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    10561 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    10621 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    10681 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    10741 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    10801 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    10861 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    10921 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    10981 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    11041 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    11101 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    11161 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    11221 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DAZKCE010000099        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000099 DAZKCE010000000
VERSION     DAZKCE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.100"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8KQ_002291"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8KQ_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9105199.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8KQ_002292"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8KQ_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9105200.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8KQ_002293"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8KQ_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9105201.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8KQ_002294"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8KQ_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9105202.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8KQ_002295"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8KQ_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9105203.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8KQ_002296"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8KQ_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9105204.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8KQ_002297"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8KQ_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9105205.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8KQ_002298"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8KQ_002298"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9105206.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8KQ_002299"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8KQ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105207.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8KQ_002300"
     CDS             9295..9939
                     /locus_tag="ACG8KQ_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9105208.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8KQ_002301"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8KQ_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9105209.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8KQ_002302"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8KQ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105210.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKCE010000100        10751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000100 DAZKCE010000000
VERSION     DAZKCE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.101"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..588)
                     /locus_tag="ACG8KQ_002303"
     CDS             complement(<1..588)
                     /locus_tag="ACG8KQ_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9105211.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYI"
     gene            complement(1156..2223)
                     /locus_tag="ACG8KQ_002304"
     CDS             complement(1156..2223)
                     /locus_tag="ACG8KQ_002304"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9105212.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2243..2722)
                     /gene="pssE"
                     /locus_tag="ACG8KQ_002305"
     CDS             complement(2243..2722)
                     /gene="pssE"
                     /locus_tag="ACG8KQ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFP9105213.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2739..3200)
                     /gene="pssD"
                     /locus_tag="ACG8KQ_002306"
     CDS             complement(2739..3200)
                     /gene="pssD"
                     /locus_tag="ACG8KQ_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFP9105214.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3214..4464)
                     /locus_tag="ACG8KQ_002307"
     CDS             complement(3214..4464)
                     /locus_tag="ACG8KQ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFP9105215.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4842..6293)
                     /locus_tag="ACG8KQ_002308"
     CDS             complement(4842..6293)
                     /locus_tag="ACG8KQ_002308"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9105216.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6614..7378)
                     /locus_tag="ACG8KQ_002309"
     CDS             complement(6614..7378)
                     /locus_tag="ACG8KQ_002309"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9105217.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7406..8104)
                     /locus_tag="ACG8KQ_002310"
     CDS             complement(7406..8104)
                     /locus_tag="ACG8KQ_002310"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9105218.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8116..8895)
                     /locus_tag="ACG8KQ_002311"
     CDS             complement(8116..8895)
                     /locus_tag="ACG8KQ_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9105219.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8911..9855)
                     /locus_tag="ACG8KQ_002312"
     CDS             complement(8911..9855)
                     /locus_tag="ACG8KQ_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9105220.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9901..10680)
                     /locus_tag="ACG8KQ_002313"
     CDS             complement(9901..10680)
                     /locus_tag="ACG8KQ_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105221.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         3127 a         2235 c         1592 g         3797 t
ORIGIN      
        1 tatataatcc ctttctgacg gaaacttgat attgtgctgt tgaattatct cagatgaata
       61 cagcgcatgc caaactgaca ttggcaatat aaaatcgact ttataactcg gctcagtgcc
      121 aatcattcca agcaacactt tttcaattat agcgttattt gtaaatatcg tactatcata
      181 tcttgctggc tttgaaataa tattattatc atcataatat tcataatatc cagcataaca
      241 tgtgtctgca ttactccttt ttataccatt taacattgct tcaatataat tagctgcaac
      301 aaaatcatca gaatcaataa aagcaacata ttttcctttt acatgttcaa gtcctgtatt
      361 tcttgccaat cccaagccag cattattttt atgaataata cgaatccttg aatcctttaa
      421 tgcatagtct tcacatattt tattagatga atccttagat ccatcatcca caattataat
      481 atctaggttc ttatatgttt gattacacac tgattccaaa caacttccta catacttttc
      541 aacattatat acaggtatga ttacactaat tagatcactc gaattcatcc ttctatcctt
      601 tcactcacgt tttctcaatt agtcaatgac ggcatcaacg attttcttaa attctctgaa
      661 aaaaatagac tctgatcttg atgatgtgaa tgacatacta tcttcttcta gaaaaattat
      721 ttcctttaaa ttatttatta aatcaataat atgatcctcg taagtatgct ttcccttgga
      781 ataatgaaaa tatacttttg tattacacaa tattgcaatt ttatttctta atatgattat
      841 ttcactcatt cattactgcc acatatacac aacttataac caccccctgc aaagtttagg
      901 gtaagttatc agataatatc ttcctcttca tcatccagaa tacaaaatct atttatttat
      961 agaaatatca ttaatgtttt tatataatgt accgtgcttg tactcttgct ctattacaag
     1021 cactaaaaca ctactattca tttgaattct tacacggata aaaatttaca tcgtctattt
     1081 caatattttt tctctctatt ttattcactt aataagttca ttacataatc gaaacatttt
     1141 ttattttcct tgtcatcact ccttgctccc tttttgatac agatttaaaa ttctatcaaa
     1201 gaaatttcca tcgttaaatt tcgttattgc caaatccttc gaacaacgtg aaaattttac
     1261 gtatttatct ttatcattga tttttagtat tgcagatgtc aacgctgata cgtcaccagg
     1321 ttgaaacaaa tatccatttt ctccatcacg tactgctgaa gaaatatctc ctacatctgt
     1381 agcaattaca ggcattccat aagaaatcgc ctctagaata gatataggca atccctcgtt
     1441 ataggacggt aaaatcatca catttgatct tctatagcac tcctcctttt tttctcccac
     1501 aacccaacct gtaaattcaa aatacgattg caaattatat tcttctatca attctttgag
     1561 cgatatttct tcatcacctg ctccagcaat ctcaaaaata aacttttttt ctggatattg
     1621 ttctgcaatg tttttaactg cttctaccaa atcgtgtaca ccttttcgct ttatcaaaac
     1681 tcccataaac agaaatcggc acatttttga attccacttt atggtatatt ctggaactac
     1741 aatagaattt tctacaacaa cagttttaca atttggctca atttctttaa ttatcttctt
     1801 ccatttttct ccaagtacaa tcgtggaatc agactctctt aacagcaagc gaatctgatt
     1861 ttgttttttt acagaaacac tatcatacca ttttttaaat tccgatccat gaagatggat
     1921 gattacagga atatttaata atctaaacca cttattcagc acatatttcc tactaaaact
     1981 ccctctgtac gacatgtgca tatgaataat attaggttta ctcaataaag agatacctat
     2041 aattttagca aatgcataca taaaatataa agatttaaat attgcatttc cacccttata
     2101 actcggtata aaagtcatct cgatattctt tttttcccaa ttaaaattga gcatttgtgt
     2161 aattatactt gtaattccac cttttaccga gctgtcatta ccaatcataa gtactctttg
     2221 cataacgctt tactccttat atttatattt tttctattaa tacctctatc gaattaataa
     2281 tcgtttccgt gttagataca tacttgttaa acatccgaac tcttgtttct ttaacggcat
     2341 cttctaaatt ttctaattca taacaaggat agataatatt catctcatca aattgtctaa
     2401 ttaactgcag ttggtgatca tcgacatgct caccatactt agccaatcga ggaacagcaa
     2461 tgaccctttt accttttttt acggccccaa ttatagcacc tgtacctcca tgagtaatca
     2521 ccatttctgc tttttgcata gtttcagtaa actcatccct atttagaaat gctttatagg
     2581 aataatttag tggtttataa tcactataac cactctgtgc aaatgtactt tcgattatta
     2641 ctcctttttc aattaaatca tcaatagctt ttaacaatct atcaaattga aatttttgtg
     2701 aacctaatgt tataaatatc atttttttct cctattattt aatatatccc acccaaataa
     2761 atagcattag gataaaattg tagcatagtt tcccactgaa cataaaattg atctgcaaaa
     2821 tgatacatca attttcctgt ctgagtaggg ctggttactt ttgcaaacga ttcaatataa
     2881 atcagttttt ttcccatcat ttttgttatt aagcagatag ggatcatagc taaaacacct
     2941 gtacaaatca cgagatccgg tttttccttt atatatataa taattgattt gaataaatta
     3001 ataagtatag tacacaaaaa aaacttttct tttctattta cttgtctcat gtagtacgtt
     3061 ttttcgcctt ttgactcagc tgaatattct gtctgttctg ttataacaaa actctcatac
     3121 ttctccatca gtggttttaa catcattaac tgttcataat gccccccaga ggaagccgca
     3181 aaacatatct ttttgttcat attaacctct ctatcagtca cgctgaaaaa tatctctagt
     3241 gtaaactttt tctttaatat catccaagca ggtatcatat cgattggcaa taatagaatc
     3301 acacatcgcc ttaaactgct ctaaatcgtt gataacctta cttccgaaaa aagtctctcc
     3361 atctttcagt gcaggctcat aaatcaccac agtcgcacct ttagctttta cgcgcttcat
     3421 aactccctgg attgaactct gacgaaaatt atcactgttg ctcttcatgg tcagccggta
     3481 tataccgatt aaaactggat tttccctgtt gacatcatac tgattatatt cagcgtagtt
     3541 gtaatagccc gccatctgaa gaacacgatc tgcgatgaaa tccttacggg tacgattaga
     3601 ttcaacgata gcctcaatca aatttccagg aacatctgca tagtttgcca acaactgctt
     3661 ggtatctttt ggcaagcaat aacctccata tccaaaggat ggattgttgt agtgagttcc
     3721 aatacgagga tcgagacaaa caccattgat tatctgctga gtattcagcc ccttcatttc
     3781 agcataagtg tctaattcat tgaaatagct gactcgcagt gctaaatagg tatttgcaaa
     3841 cagctttact gcttcggctt cagtgaatcc cataaaaaga gtatccacgt tctccttaat
     3901 tgcacttttc tgcagaagtg ttgcaaaaac atgagcagca tttaacaagc gctcatcagt
     3961 catatcagta ccaacaataa tacgagatgg atacagatta tcatataaag ccttgctctc
     4021 gcgcagaaac tctggactaa aaataatgtt cttactcccc gttgtctcac ggactgattc
     4081 agtgtagcca acaggaattg tagacttgat gaccatgatg gtatcaggat tcacgtccat
     4141 cacaacctca ataactgact caacagcaga agtatcgaaa tgctgagtac gactgtcata
     4201 gttagtcggt gtagcaataa caacgaaatc tgcggattta taagcgctcc ttgcatccaa
     4261 agtagcagtt aagtcaagtt ctttttctgt aagatacttt tcaatatatt catcctgaat
     4321 tggagacttc cgtttattaa tcagttcaat cttttcaggg ataatgtcta ccgcggttac
     4381 ctggtgatgc tgcgccaata gtgtggcgat actcaaacca acataaccgg tgccggctac
     4441 tgcgatgttg tactttttct tcacaatatc tcctctttcc tctcttaagt atggagaatg
     4501 ttactcctac ggctattcat catgtcaaaa taacacagct tagaatgttg ataaaataat
     4561 tttccagaat gtgataaaat ctctttcatt tataaaccac acatctaatt ttatctattc
     4621 tccacaagac atgtagagta ttctttaata acaagatacg accttactaa atacttcaaa
     4681 aaattatcat atttaattca taaatataaa tcttcatttt aaatgtatgc ccaaaaattt
     4741 atctgtaatg cgtcatcaaa atctagattc caaatcttta tcaattttta tccactcatt
     4801 ctaccacttt agtaatattt tctatttata acactttcgt cctacattga tccccccctc
     4861 tgaagtacag tttttactgt cttaaataaa attttaaaat ctaaccccat actccaattt
     4921 tttatatatt ccgtgtcaag ttttgctact tcttcaaaat cagtaatatc acttctacca
     4981 cttacctgcc acattccagt aattcctggc ttaatagcaa ttcttgcacg atgatgataa
     5041 tcatattctt catactctga tttaaggcat ggtcgaaatc ctataaggct catctttcct
     5101 gttagaacgt tgaaaaattg agggaattca tctaaagacg tggatctaat aaattgaccg
     5161 attcctgtct tttttgttcc atctggaagt attttatttc cgataattct tggatcaaac
     5221 tccatcttaa acataagatt attgctcatc ttattttctt tcattaattc tacttttctt
     5281 tcttctgcat ccatatacat acttctaaac ttatacatct tgaacggttt accattctga
     5341 cctatacgtt cttgtgcaaa gaaaattggg cctggtgaat taatataaat aagtggagca
     5401 ataaagataa aaataattcc cgttgcaata catccaacaa tacctgcaag aatatcaatt
     5461 gttcgtttta taaatgcctg ttgagttgtc ataacattga tacttgttgt aagtactgta
     5521 tattctccta tacgaccaat agattgtttt cccaaagcac tatctgatac ttttgcaaga
     5581 ttcaaatgta cagtaaggcc catttcagag catcgttcaa taaattcttt catacacgaa
     5641 gtacttttat tcaaaataat aaagacttca tctacccatc cctgacataa gtaatccgct
     5701 gcatttttgg aatcagaaac aactgtcact ccctcaacat cttcaccaat catgctttta
     5761 tctatgataa tgacaccatt tattttatac attttatagt tatgctcttt cacattacga
     5821 ataactactt cagcaatact actagatgta actataataa gtgatttgtt cccctcattt
     5881 ttcattttat gtttcaagta atgtttccat aagaggcgag ttatatatgt aattacaacg
     5941 tataaacctc ccatcaagaa aagaacgact ctagaataat catttccacc ctgaacacta
     6001 atgagataca aacttcctaa cagcaccaac ataatcgctt gctttacaga agaagcaaat
     6061 tctatatagt accctctttt taatacacct ctatatgctt caaacatgaa aataactaaa
     6121 agatctgcca gttcaatgaa gactgccatg ttttgataca ttggaatcac ataaggattc
     6181 caatctacct ggcgtaagac atacgataga taaaaagcaa gctggataca caataaatct
     6241 aaaataataa aatcaatatg tttaacccat ccccttgaac ttttcttgta cattcctttt
     6301 tactcccctc tttttaataa aataaattgt attacttgtt ttctctccta aatatctttc
     6361 tgaaggatta ttgactcgac aaattggctt gtggatattc ttatatatag aacatccgta
     6421 agccaactta aaactcctta acaaaatttt tttactcata ttgtagatca aaacaagtat
     6481 ccaagaaatc atattttatt tcttttttct gcttactcaa caacagctaa aagaaaaatc
     6541 atcataaaca aattcaacta agccaacgat ccccttgtga aatcaagttt tttcttgatt
     6601 tacgaataaa gagctaaaat aaaccaaact tcttcttttt tacctcttca tagactggat
     6661 aactgatttc atccccgttg atcagatcct ttgcgacttg tttcatctgt actacttttt
     6721 ccttaccaaa gtcctttgca atctgctcat agcactcttt taagtaaaag gtgcgcttag
     6781 agaccccatg ggcatcagaa gcagccatct gcacgagatt atgtttgacc atcgttttgg
     6841 ctgttttctg gatgtctttg ccaaataccc caacataact tggcgcagtg agttgggcca
     6901 aacatcccat atctaggaac ggaagcaaat gatttgggtc ttcgcgaaaa tgcacgttgc
     6961 gttctggatg gacgattact ggtgtctttc ctagttctaa caactcaaaa aaaagccgtt
     7021 ctgcatataa cggcacttcc agtgtcggaa attcgatcag aagatatgta tcatctaaat
     7081 ctgtaaataa tatttcatct cggtggatgt cttcgatgag cgttccggta atacgtactt
     7141 cttgtccttc caataacgtc aaaggcaact gtcttttctc taattcggct tgtaacgaag
     7201 cgactagaga aatgacttgt gatttttcat tgctgtattt tccattattg tggtgtggtg
     7261 tgcataaaat atgcgtgatg ccttgctgaa tcgctttttc agccattgca atacttgctt
     7321 ctaagttttc tgccccgtca tcgattcctg gtaggatatg gcaatgtaga tcgatcattt
     7381 ctcttgtctc ctctcttctt attccttaat ttccgtagta ataaccagca tctttgctat
     7441 gttctgctcc gttgtacaca acacctaaga ctcgtgcttg taccatctcc agcagttctt
     7501 ttgctttagt caaggattct ttacgtgaca tattttcacg tacgaccaat aaagtgccat
     7561 ccgtttttaa agacatgatc tgtgcatctg ttacggctac tactggtggc atatcgaata
     7621 tcaccacgtc aaataactgt cttgcttcgg ataggatctg atccattctt ggcgagctta
     7681 acaattctga tgggttgggt ggcttaggtc cactaggcaa aatcgacaag ttctcgactg
     7741 gcgttcgttg gatcacatct gctacacttc ctgatgaact aagtgctgta cttaatccac
     7801 tggcattgtt cagctggaat gtcttataga cgacgggttt acgtaaatct gcatcgacta
     7861 gtagaactcg ttgacctgat ttagcaaaaa cgactgcaat gtttgctgca gtggtggatt
     7921 tcccttctcc aggacctgat gaagtcacga caatcgtttt gatttgctga ccagctgcgg
     7981 aggcaaactg gatattcgtc cggatcgtac gatattgttc ggcgattggt gaagatgggt
     8041 ttgttaatgt gatcaaactg actgcatgag tttgtgctgc tttttgtttc tgtgttcgtg
     8101 ccatttttct tcctcctaga ctcttgagcg gcttctgcgt gatggggctg ttttatcgtt
     8161 tgtagctttt ggtcgtgcag gagctgttcc ttttttacgg ataccagcgt tcaattcttt
     8221 cgctgacatt tctggaactg ttcctaagat tgggaatcct aatgtatctg tcacatattt
     8281 ttcatctttc actgtccgat cgagtaattc caataagaat gccagaccta cacctaccat
     8341 cattcctaac attaagccag tcattaaatt caatttatta tttggcgaaa caggtgtcgt
     8401 attggcaact gcacctgaaa tgatcgagat tttatcaaca ttcatcacat cttttgcgtt
     8461 ctcttggaaa acttgtgaag ttgtgttagc gatctgttga gcagccactg cattcgtact
     8521 tattgcctga atcgagaaca tttgtgaatt ttctgcctga ttaactgaga tggcatcttt
     8581 gatttgttca gcagtcattt tcacattgtc ttctgtttct aaacgatctt tgacttcatt
     8641 gagcactaaa tcactgacga tcatatcttt atacgtattg atcatttgca ggttcgtatt
     8701 gacatcattg acatttgcag tgtctgattg tggcagtgtc acgatcaatt gtgcttgtga
     8761 actgtatttt ggtgtcaaga tgaaaaatgt tacgattcct gagatggcca agccagcaaa
     8821 cattgagatg atgattatta ccaagtgttt gcgtaatgtt tcaaaaattt ctttcaagct
     8881 gattgtctct tccatttttt ctccactttc ctatttattc tctagattca tttgatcttt
     8941 taattctttt ttcacacgat caagttcttg atcactgact ttttgatagg agatattatc
     9001 ttgcatgaag ccttctcctt gcagctgatc ttgcttaatc gtaccaaatg cctcccgata
     9061 atctaaagca atttttttca tttcatcaaa gttcatgtct gttttcatat tcgactctag
     9121 agcattcaaa acggattgat agttgctgac accgtttaga ctcaatactt ttttcgctac
     9181 gccttctacg atctttcttt ggcgcccttg tctaccgtaa tcaccatttg ggtcttcata
     9241 acgcatacga gaataagata atgcctgatc cccgttcaat gtaattttac cgattggaaa
     9301 gtcatagcca tcttgtgaga aagtgaggtc attattgact tcgataccac caactgcatc
     9361 aacgagttcc tttagtcctt tcatattgat agagacatag tgatcgattg ggatgtctac
     9421 atatttttgt acggtatcca tcgccatggc agctccgccg aatgcgtaag cgtggttgat
     9481 cttgtctttt gtgtcacgac ctacgatgtc gacatacgta tctcgaggaa tgctgacgat
     9541 cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc atcgtatctg atcttccttg
     9601 atcgacccga cctaaatctc cagtatcaat ccccatcaat agaacagaga aagattcttg
     9661 tttgtccaga tcaactggtt tcgttcgttc tccttgggtt cgttccactg attcgtaagt
     9721 tttctcaata gaatgtgaga gatccagata taattttgct cctactccta caattgctaa
     9781 tactaatacg ataagaactg ctaaaataat caaaaatatt tttttccctc ttgattttct
     9841 tcttcgtctt gccatagttt cctccgtttg ctaaccttgc ttgaatcaac gttttaactg
     9901 tcaatagctt atattgctat agcccatttg ctgagcaaca gtttgtgctt ttgttgcgta
     9961 tgcggctctt attttttcat tccggatctg ctctatttga ctgagcgccg tcaaatattg
    10021 atcgtaagta gcggtagctg ttacttgacc gtctttcagc atcgtatcga atagctgatc
    10081 tagttgcgtg acttgtgcaa tttgggtatc agcgaatcct agatattgca taacgactgt
    10141 tttccaagca ctatccttaa aaaagctcat attttcacgt acatgggcga catctgcact
    10201 tgccaacttt tctttgatga tgacatcatc cacagcttgc tgccagtttg atacgttttt
    10261 agtaaataat ttattgacag ccgtctggat cttcagtttt gattctgcat cctctaactg
    10321 cgttaagact gctttctttt cttcttggat cgcagcagct tctttaggta attctgattc
    10381 cttgatgtca taatcatctg ccgaaagttt gatagaagat agtttatgtg acaaatttga
    10441 aactgtttct tctgttattt caggatttag gaaaattttt tcttttgtac tgtacagctt
    10501 atctacttct tttgataaag aggtcacggt gtctttttga ttggtgatgg cagattctgc
    10561 gtaattgatc cgttgatgat attgccgctg attattgaga tggtatccaa acaagcaaat
    10621 cacaatagcg cctgtgccta ccgccacagt agccaataac ttgacgaacg gcttttccat
    10681 agattcactc ctaaatattg atgatagata agtaaagatg tgattcattc acatcagaat
    10741 gagcgctttt t
//
LOCUS       DAZKCE010000101        10745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000101 DAZKCE010000000
VERSION     DAZKCE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.102"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(288..1136)
                     /locus_tag="ACG8KQ_002314"
     CDS             complement(288..1136)
                     /locus_tag="ACG8KQ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9105222.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1300..2202
                     /gene="gnd"
                     /locus_tag="ACG8KQ_002315"
     CDS             1300..2202
                     /gene="gnd"
                     /locus_tag="ACG8KQ_002315"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP9105223.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2204..3205
                     /locus_tag="ACG8KQ_002316"
     CDS             2204..3205
                     /locus_tag="ACG8KQ_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP9105224.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3202..3486
                     /locus_tag="ACG8KQ_002317"
     CDS             3202..3486
                     /locus_tag="ACG8KQ_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9105225.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3519..4316
                     /locus_tag="ACG8KQ_002318"
     CDS             3519..4316
                     /locus_tag="ACG8KQ_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9105226.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4330..5163
                     /locus_tag="ACG8KQ_002319"
     CDS             4330..5163
                     /locus_tag="ACG8KQ_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9105227.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5181..6038
                     /locus_tag="ACG8KQ_002320"
     CDS             5181..6038
                     /locus_tag="ACG8KQ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9105228.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6060..6569
                     /locus_tag="ACG8KQ_002321"
     CDS             6060..6569
                     /locus_tag="ACG8KQ_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9105229.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6606..7007
                     /locus_tag="ACG8KQ_002322"
     CDS             6606..7007
                     /locus_tag="ACG8KQ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105230.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7119..7472
                     /locus_tag="ACG8KQ_002323"
     CDS             7119..7472
                     /locus_tag="ACG8KQ_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105231.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7506..7949
                     /locus_tag="ACG8KQ_002324"
     CDS             7506..7949
                     /locus_tag="ACG8KQ_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9105232.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7966..9051
                     /gene="uxuA"
                     /locus_tag="ACG8KQ_002325"
     CDS             7966..9051
                     /gene="uxuA"
                     /locus_tag="ACG8KQ_002325"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9105233.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9044..9757
                     /locus_tag="ACG8KQ_002326"
     CDS             9044..9757
                     /locus_tag="ACG8KQ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9105234.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9964..10113)
                     /locus_tag="ACG8KQ_002327"
     CDS             complement(9964..10113)
                     /locus_tag="ACG8KQ_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105235.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10509..10709
                     /locus_tag="ACG8KQ_002328"
     CDS             10509..10709
                     /locus_tag="ACG8KQ_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9105236.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3602 a         1696 c         2316 g         3131 t
ORIGIN      
        1 tacttaattc accaaaattt tttttaattt tttagaaaat aagaaacaaa aataataaaa
       61 aaaagaaaag ataaattttt tactactgta tactaacttt gttatcgcat ctaacaagta
      121 actcgaataa taccaataca accttctcca aagatgcgat gacatacgtt ctaacccacc
      181 gactattgat gttctgtacc acccttgcag aaccaaaaaa gccactacca aatatttggt
      241 agtggctttt tttgttttat ggatttttcg ctttttctgt tatggtgcta ttctttgatt
      301 tcagaaatag tatgtcgaac tttagcgata gaacgacgtg attctttttc gttgtaaaac
      361 atcaaaatca catatagaga gtccaaaatg cttagttgag aaatacggga agctaaagct
      421 tcagatcgga attctgtttc ttcagaaata gaaataaaaa cgacatcgcc aagtttagca
      481 agggaagaat tagcttggct agtaataacg attgtttttg ctccagtttt tcttacggtt
      541 tcagcaatat ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac agcacagtct
      601 ttttccgtca ataaagaagc ttccatcaat tgaatatggt aatccgtgct gtgagaagtt
      661 gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc tgaaccgcca
      721 acaccgaaaa aatagacacg tttagattca ttgataatag tagccgcttt ttttagatcc
      781 ttttgttgta acaacttttt tgtgtcattt aaagttgaaa tactagactc gaacaccttt
      841 tgcgccatag tgagctcagt atctgttttc tgaatatttt cgtggataga aatagcagat
      901 agatcatttt cttggatcaa catggccatt ttaaaatctt taaatccatt ataaccaagt
      961 ttttttgtaa attgaaaaaa agtagagtca gcaatgccca gttcacttgc taaattgcta
     1021 ataggactat gacttacttt gcttggattt tctaaaatat aatcggcgat agcctgttct
     1081 tttgggctga agtcgttgta cattgttttg atttgtagtc ttatggagac gctcatgaga
     1141 attcctcatt ttcataataa gattatcatt ttttccgttc tcattaatgt taaagataca
     1201 aggagagtag gaaaaaatca aacaaaaaat atttttgtaa aatctattta caaaaaaatt
     1261 tttttgtata atgagggtat aagaaatgga ggcgctatca tggaaattgg tgtaattggt
     1321 ttgggaaaaa tgggattgaa tttagcttta aacttaaaag agcaccatca caaagtacaa
     1381 ggactcgaca tttcaagtac agctgttagt caggcaagag aaaatgacat tcctgcttat
     1441 caagagatct ctgaatttct agctactttt gagcagaaaa aaatcatctg gttaatgctt
     1501 cctgctggag agataaccga aaacatgtta agaaaacttt ttccaaaact cggaccagga
     1561 gacattatta ttgaaggcgg aaactcttat tataaagatt caatccgtcg agcagaagag
     1621 tttcagaaaa atgaaatcgg ttacttggat tgtgggactt ctggaggaat cgaaggagca
     1681 cgacatcaag cctgcttaat gattggagga gataaagaaa cttttctatc cgtggaacaa
     1741 ttattcaaag atgtagcaat tgaaagagga tatctgtatg ctggtgcatc cggaagcgga
     1801 cacttcttga aaatgatcca taatggaatc gaatatggaa tgatgcaagc aatcggtgaa
     1861 ggattccaac ttgtacaaaa aagtgagtat gcttttgact tggaacaagt cgcacgtgtt
     1921 tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga tcagttaaac
     1981 gaacatccca atttaagtaa ttatcgggga atcgtttctg catcaggtga agcaaaatgg
     2041 acggttgaaa cagcgctaga aatggctgtt ccagttccag taattgcaac tagtttgttc
     2101 atgaggaatc tttcacagga agaagatagt ttttcagcaa aagtagtttc agcactaaga
     2161 aatgggtttg gcggacatga aatagttgaa aagggtgagt aaaatgccag attatcatat
     2221 agcaatcgtt aattccagca gtttcggaag agtctttcca caccacttgg aaagattaaa
     2281 gtcagttgga gacgttcatt tctttactgt tgatcaagag attccaggaa aagaactagc
     2341 ggagttactt aaaggattca atatcattat tgcaagtgtt acgccatttt ttcgacgaga
     2401 tttttttgaa aacaaagatg aactgctatt gatttcccga catggtattg gttacaacaa
     2461 tattgattta aaagcagcaa aagaacacca gacaatcgta tctatcatac ctgctttagt
     2521 tgaacgaaat gctgtagcag aaaataatat aactaatttg ttgtccatgc ttcgacgcac
     2581 gacacaagca caagcaagag tcaaggacga tcgatgggaa gatcgggcgg agttcgtcgg
     2641 taggacgctg tttaacaaaa cagttggtgt gattggtgca ggaaataccg gaagcggagt
     2701 aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc cttacaaatc
     2761 agatttatac atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat tactggaaca
     2821 atcagatatc atttgtttat gtgcgaattt gacagaagag aactatcaca tgatttctga
     2881 taaagaagtc gatcagatga aagaaaatgt ttatctatct aatagtgcaa gaggcgctct
     2941 agttgatgaa gaagcgatga ttaatggtct gaaaagcgga aagatcgcag gatatgccac
     3001 agatgtttta gaagaagaac cgggacgtca agatcatccg tatttagcgt ttgaacaagt
     3061 agtgattacg ccacatatat cggcctacac aagggaatgt ttagaagcaa tgggtgaaaa
     3121 atgtgtcaga gatgtcgaac agatcagtca gcaccttctt cctgaacgtt ctgtccagac
     3181 aaagagcaac tatataaaag gatgatgaag aaaatggtga acggtttaaa agtgaaaaca
     3241 ggccctcaat tttatttata tgaagaaggt ggtatttcaa aagtttctga tctcttgaaa
     3301 tcatacggcg cgaaaagagt actggtcacc cacggaacgg tatcatggga aaaggcgcta
     3361 cctaaattag tatttttgaa tgacgaaacg attcaatttt tttatcatcg ttattctgga
     3421 gaatgtagtt atgctgaagc cagacgaata gctacaatca tcaaaaaaat gaaatcgatt
     3481 tcgtagttgg cgttggcgga ggaaaactga ccgatctagt gggttatagt gccaatcttg
     3541 caagaatcga tttcggagtc atccctactt tagctagtaa ttgtgctcca tggacacctc
     3601 ttgctgtcat gtataaggag aatggacaag cagaagggaa aacagaacac ttttacagac
     3661 aagctgcttt ttttataaca gatccccaat tagtgattga ttcccctgtt ccatatttca
     3721 ttgccgggtt agcagataca ctagcaaaat ggtatgaatc tgagctcatc ttagaacaag
     3781 attttttgca agatgaatca tttttgaagc tagcacaaga tactgcgaag atctgcaaag
     3841 aagagatttt gaatcattct gctaaagcaa tccaagatat ggcacaaaga aaactgacat
     3901 cggagttcaa acagctttca gagattatct ttgctgttgc tggtttagtt ggaggattag
     3961 gagataaata cgcaagaaat gtggcagcac atgctatgca tgacgctatc agtaaatata
     4021 ttccggaaag ccatcgtttt ctacatggag aaaaagtagc gtatgggatt ttctatcaac
     4081 tggcactaga aggtcgctgg gaaaaaatag acgagttata tcctttgtat cgggaattag
     4141 aactgccggt ttctctagaa caaatgggat tatttccgaa agataaacag ataatagata
     4201 aaatggctga atttattgat tcgaaagaga aagtgcattt gattcctata gaggtaacac
     4261 agaaatcact gatacagtca atgatggaac tggaaggtta tatcagaaag atctgaagga
     4321 ggaggacaca tggaaacatt aagtgtagta caaagtttgt tgattgcttt atgggtagcc
     4381 gcggttatgt cccgctggtt aggtggagga gccacgttga ccttacgttt ttctcctttg
     4441 atgactggat tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat gattgttaca
     4501 gccgctttgc aaatgattta tatgggggtt ttttctccag gaggatcaat gccagctgaa
     4561 ccatctattg cagcagctat cgcagtacca gtcgcattat taggggatct gtcgccagaa
     4621 gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa gttacttgta tcagtttcgt
     4681 tttttcatca atactttttt aggtaaatat acagacaaag ctgtagaaga tttgaataca
     4741 aaagctatgg cccgatcaat tattctttat ccaacggttg cctcattttt attattcgtt
     4801 ccgcttgttt tcattgcttt atatttaggg gcgccattga tcgctgatgt aatcaaagct
     4861 ttagagggaa cagtcgttat ccacattctt gaagtagtag gtggagggtt agcggcaatc
     4921 ggtatcgcga caactgtata cgttatcggt cgaaaagatt atctggtctt tttcttcctt
     4981 gcttatttta tgagtgttgt tttgaaatct ttagatatta cgatggttac ttatgccatt
     5041 tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca agtacagcct
     5101 gccacaagca ataacaattc agcatctttt gatgacgaag atgactatga tgacggattt
     5161 taatttagga ggaatgggaa atggctgaaa taatggataa tcaaacaggt gctcctgaag
     5221 aaatcacgaa aaaagatgta acaaaagcct atctgagatg gcattttgca aacgaaattc
     5281 ctcactcttt tgaacgttac ttggcgccat cactactata cggaatgatg ccaatcttga
     5341 aaaaacttta taaagatgaa gactctttaa aagcagcata taaacggcag ttactgttct
     5401 tcaacactca attaagctgg ggtggcggtg tgattactgg cctaatgtct tctatggaac
     5461 agcaaagagc agaagaagag tatgaagaaa aagagatctt gatgcaggat gacttgatgt
     5521 ataacacgaa agcgggattg atgggggcac tagcaggaat cggagatgca atcgattcag
     5581 gaaccgtcca atatattttt atcgcaatcg cggtaccttg ggcacaacag ggaagtcctt
     5641 taggcgcact ttttcctttt atctgctttg ggttgtatca ggttttacta ggggtatttt
     5701 tcgcaagaca agctttcact atgggccgta atgctacagg cctaaagcaa aacactggtg
     5761 ttcagacagc gattgaaatg ctatcagttt tagggttgtt tatgatgggg gtgcttgcgg
     5821 gaaactatgt aaaagtagaa tcttcattga aatttgcgat atccggacgt gagtttgtta
     5881 tccaggaaat tcttgatcaa atcgtgccag ggatattacc tttagctgtc gtaatgggtg
     5941 tgtacttgtt ctatacgaaa aaaggattga aagtcacaca agcattatta tggctgactg
     6001 gaatattaat tgtgctagca ggaatcggta ttttataaaa agaagggaac gtgaataaga
     6061 tgggtgtagt caatttagcg cgtgtggatg agcgtttgat ccatgggcaa gtaatggtaa
     6121 cgttatctca aaaaagtgga gtgaattcca tttttgttgt agacgaagtt gtagcaaaag
     6181 ataaatttat gagagatctt tacaaaaatg caggaagccg tacaggacaa aaaacaattg
     6241 tcattacacc agagaaagct aaattctatt gggatgaata tcaatttaaa gaatacaatt
     6301 gtattttgat cgcaaaaaca gtccaaacga tttacgactt agtaaaacac ggaatcccca
     6361 tggaagaatt gaatattgga gggattgctc aaaaaaatcc tgatacagat cttttagtga
     6421 cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg aatgaacaat
     6481 atggcgtaaa gaatatttat tttcaagcca ctccatctgc acctaaaaca ggattaaaag
     6541 atgtattgaa caagtttgat ttgtcataaa caaatgctta gaaaaaagat aaagagggcg
     6601 aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc tatatttacc
     6661 gtaagcgccg tacaaaaaag agtaaacagc gttttctttc agcattagta tcagatattt
     6721 acagtatgag aacagatgtg acagtcattg cttacgatac gctagcctac cgatcaaaaa
     6781 atatttatgt tggagatatc gaaaaagcag agaaagttat ctgtacttat tacgatacac
     6841 ccgttcatgc attaggaagt tatttcatgt ttgattggaa agaccaaagg aaaaaaacaa
     6901 tctactctat attattgtcc ttcattttgt tgttcagttt aggttggtgg ggaatgatga
     6961 tatataacaa aaatcctcat cacgtattcg atctgctatc cgtataaaca ggtatcactg
     7021 tactagcatt tgggagctac tttttcttat tgggaaaagc agctcgcgga tggcctagca
     7081 gacaaacttt tattcgaaat acttcatcca tattaacgat gctagaaatg atccgtacga
     7141 ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga gaaaagggct
     7201 tagacagcgt gagatcgggt atgaaaaaag aggctattct tttttatcta gactctgttg
     7261 gggcagatac accgctacag ttttcgggaa attatttttc aaataaagaa cagtggctga
     7321 aacaagttga taagttgaaa gaaaaaaacg tcaattatat ttttagtgca agaaaaaagc
     7381 aagcgcagtt cttctatttg accaaaacag atttacgagg gaaaacattt aattggcaaa
     7441 atgccaatca gattattgct ctttttcgat aagtaaaaag tgcaaaacaa ggagtgagaa
     7501 gactaatgtt ggaaatcatg atcgttacac atggtaagtt gagcgaagga ctgaaagatg
     7561 cggcggaagt aattgtcggg acatcgagcc atattgaaac aatgtgctta gagtctgggg
     7621 aggacatcca aacattcgga gagaaggtca aagaaaaatt aactatgatc aacacagcag
     7681 aacccatgct agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa gcgctcttgg
     7741 cagtcaatga actgactgaa gaaaagaaag cagccactta tgtcataggg ggcgtgaatc
     7801 tgccaatgtt gttagaagca atcaatcatc agatgcttca cacaccgata gaagaagcgg
     7861 tggaacaaat tgtcaaacaa ggaaaagata atttaactgt ttggcatgct tcagcggaag
     7921 aaaaaaaatt agatgaggac gatttttaga aggaaggctg aaaagatgaa gtggggattt
     7981 agatggtatg gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca aataccggga
     8041 ataaatggga taattggaac tttgctgact aaaatgcctg gagatgtttg ggagatttca
     8101 gaaattgagg agttgaaagc atctgttgag agccaaggac tggaactgct tggaattgaa
     8161 agtgtagcga tccacgatgc aatcaaggca gggacagatg aacgagatta ctatattgat
     8221 aactatatac gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat ttgttatagc
     8281 ttcaaaccaa tttttggatg ggcgaaaact gatttgttta atgaaaacaa cgatggaagc
     8341 tattctcttt tatacgatca gaaaatcgtc gatcagatgg agccaggaga aatgtatcaa
     8401 ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga acggttgaaa
     8461 aaattcgctc agctggaaaa actgtatgaa ggaatgaccg aagaagaatt atttgaaaat
     8521 ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt aaaaatggct
     8581 atccatccag atgatccgcc atgggaactt tttggttttc ctagagtgac gaaaaatctt
     8641 ggcgacttga agaaaattct aaatgcagta gattctccat ataatggact aacgctgtgc
     8701 acaggatctt taggggcaaa tccggaaaac gatttagttg aaatcatcca tgaggtaggt
     8761 tcgcgcatcc attttgtcca cttcagaaac gtggagtttt taggagaaag aaagttcaaa
     8821 gaaacggcac atccctctac agaaggctcc cttgatatgt atcatattat gaaagcgttg
     8881 gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt gtggggtgaa
     8941 gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg gactcactta catgcaaggt
     9001 ctatatgagg cgataagtaa ggaaaaacca ctgggagagc attatgaata aaatcaagct
     9061 cttcatcttt gatatggatg gccttttatt agaaactgga cgtttagctt atcgtgctta
     9121 tgtaaaatca gcacaaaagt atgattatga aatgcgtaag gaagtctatt atttactaac
     9181 tggccaaaca gaaatggcga taagagaaca aatgggcatt ttgtacggag aagatgttcc
     9241 atatatcaaa tggcgagaag caatcaatca atataaagaa aaaatcgtga aagaagataa
     9301 gagagtatat acgaaaaaag gagcagaaga aattttatct tttgcaaaag aaagaggaat
     9361 ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt taaaaatgga
     9421 aaatctatat gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa aaggaaagcc
     9481 ggaaccagag atctttttga aagcttgttc aaaaatgaat attcctccaa gtgaagcact
     9541 tgtatttgaa gactcgattg cagggatcga agcagcaagg cgagcgggca ttttgtcttt
     9601 tctcatcaaa gatcatttga tagggttgcc cgatcataat gggaagcata agttgaaagt
     9661 agatgtaagt ttatatgaat ccaataaagc agactatgaa ttttatgacc tgcatcaagc
     9721 aaaacgattt ttagaaagta aagggttgta tctctaataa agaggactat tttctagtcc
     9781 tctttattta ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa ttttttattt
     9841 tgaagtatct accttatttg tagtgaacaa tttagaacaa acacaaaacg catctactgt
     9901 tatctaccag tagatgcgtt taaagagaag aacgagaatc gtccatatga gggtttccac
     9961 tccttatgca gattttttta attcttgcac tgcttgttcg atcgtaacac ctgtcgctac
    10021 atgattggta ttttctttgt caaaaacgac aaatagattc aaatctgtat ccaaggttac
    10081 tcgatattgt atatctttgt tatagaatgt catagccatt ctcctttctt taaatacttg
    10141 ctgaaaggca acgaacgcgt tgataacgaa attccattta taagtatacc accaattgaa
    10201 aggttttcat agttaagaaa ttttaaagtc aaaagttgca gataaaagca aataaacaag
    10261 aaaacaaaaa gtgaatcaat cgcggttatt caactttttc ttcgattcga agacaataga
    10321 agacaatgaa atttatattt cattgcatta tccttgtgtt attagaaaaa gcatgatata
    10381 ttaaatgagt aatctatttt gaaacgtaat ttgagcgata tatctacata gataaaactc
    10441 cttttgccta tcaaatattt attgcaacga aaaaacgtac attgatacgt atcaggagga
    10501 atttatttat gaataacggt acagtaaaat ggttcaacgc agacaaaggt tttggcttta
    10561 tcactggtga agatggaaat gatgtatttg cacatttttc agctatccaa ggtgacggct
    10621 tcaaaacttt agaagaaggc caagcagtgt cttacgatgt tgaagatggt caacgtggcc
    10681 ctcaagcaac aaacattgtt aaagcttaat aaaaattgac ttttaaccgt cttttcgaaa
    10741 gaaaa
//
LOCUS       DAZKCE010000102        10647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000102 DAZKCE010000000
VERSION     DAZKCE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.103"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8KQ_002329"
     CDS             34..657
                     /locus_tag="ACG8KQ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105237.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8KQ_002330"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8KQ_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9105238.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8KQ_002331"
     CDS             2153..3034
                     /locus_tag="ACG8KQ_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9105239.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8KQ_002332"
     CDS             3037..3807
                     /locus_tag="ACG8KQ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105240.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8KQ_002333"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8KQ_002333"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9105241.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8KQ_002334"
     CDS             5118..6281
                     /locus_tag="ACG8KQ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9105242.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8KQ_002335"
     CDS             6398..6808
                     /locus_tag="ACG8KQ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9105243.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8KQ_002336"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8KQ_002336"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9105244.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG8KQ_002337"
     CDS             9381..9665
                     /locus_tag="ACG8KQ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105245.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG8KQ_002338"
     CDS             9825..10061
                     /locus_tag="ACG8KQ_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9105246.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG8KQ_002339"
     CDS             10058..10456
                     /locus_tag="ACG8KQ_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9105247.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3721 a         1773 c         2252 g         2901 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggct
//
LOCUS       DAZKCE010000103        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000103 DAZKCE010000000
VERSION     DAZKCE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.104"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8KQ_002340"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8KQ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8KQ_002341"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8KQ_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8KQ_002342"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8KQ_002342"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9105248.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8KQ_002343"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8KQ_002343"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9105249.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8KQ_002344"
     CDS             5704..6489
                     /locus_tag="ACG8KQ_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9105250.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8KQ_002345"
     CDS             6502..7353
                     /locus_tag="ACG8KQ_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9105251.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8KQ_002346"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8KQ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105252.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8KQ_002347"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8KQ_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9105253.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8KQ_002348"
     CDS             8912..9394
                     /locus_tag="ACG8KQ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9105254.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8KQ_002349"
     CDS             9431..10441
                     /locus_tag="ACG8KQ_002349"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9105255.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKCE010000104        10181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000104 DAZKCE010000000
VERSION     DAZKCE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.105"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG8KQ_002350"
     CDS             complement(46..366)
                     /locus_tag="ACG8KQ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9105256.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG8KQ_002351"
     CDS             complement(514..2748)
                     /locus_tag="ACG8KQ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9105257.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG8KQ_002352"
     CDS             3227..3493
                     /locus_tag="ACG8KQ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9105258.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8KQ_002353"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8KQ_002353"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9105259.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG8KQ_002354"
     CDS             5384..6430
                     /locus_tag="ACG8KQ_002354"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9105260.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG8KQ_002355"
     CDS             6447..7670
                     /locus_tag="ACG8KQ_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9105261.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG8KQ_002356"
     CDS             7759..8646
                     /locus_tag="ACG8KQ_002356"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9105262.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG8KQ_002357"
     CDS             8732..8962
                     /locus_tag="ACG8KQ_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9105263.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="ACG8KQ_002358"
     CDS             complement(9019..9984)
                     /locus_tag="ACG8KQ_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9105264.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3252 a         1789 c         2051 g         3089 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag ggaattctat agttctgttc acagaaaata
    10081 ctttgataat caaaatatgt gagatagatg atttttctaa taaaagaaat tataaatgct
    10141 gatttatcag ttgttttaaa tgagtaaaat cttaaaaaaa g
//
LOCUS       DAZKCE010000105        10095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000105 DAZKCE010000000
VERSION     DAZKCE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.106"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACG8KQ_002359"
     CDS             254..733
                     /locus_tag="ACG8KQ_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9105265.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACG8KQ_002360"
     CDS             766..2994
                     /locus_tag="ACG8KQ_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9105266.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACG8KQ_002361"
     CDS             3123..4766
                     /locus_tag="ACG8KQ_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9105267.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACG8KQ_002362"
     CDS             complement(4839..5384)
                     /locus_tag="ACG8KQ_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9105268.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACG8KQ_002363"
     CDS             complement(5407..5892)
                     /locus_tag="ACG8KQ_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9105269.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACG8KQ_002364"
     CDS             complement(5882..6694)
                     /locus_tag="ACG8KQ_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9105270.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACG8KQ_002365"
     CDS             complement(6833..8365)
                     /locus_tag="ACG8KQ_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9105271.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACG8KQ_002366"
     CDS             complement(8474..9505)
                     /locus_tag="ACG8KQ_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9105272.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3375 a         1857 c         1932 g         2931 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta
     9961 tataagttgt ttgcttattg atggattgga taaattaaac catcacaacg agtggaaatt
    10021 atatgaaatt tttcataaaa attcatatag aaacaaatga cattttatta gaatatgact
    10081 ttcattaaca taaat
//
LOCUS       DAZKCE010000106        10020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000106 DAZKCE010000000
VERSION     DAZKCE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.107"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACG8KQ_002367"
     CDS             <1..83
                     /locus_tag="ACG8KQ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFP9105273.1"
                     /translation="NYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            155..1276
                     /locus_tag="ACG8KQ_002368"
     CDS             155..1276
                     /locus_tag="ACG8KQ_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9105274.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1358..3130)
                     /locus_tag="ACG8KQ_002369"
     CDS             complement(1358..3130)
                     /locus_tag="ACG8KQ_002369"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105275.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3133..4845)
                     /locus_tag="ACG8KQ_002370"
     CDS             complement(3133..4845)
                     /locus_tag="ACG8KQ_002370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105276.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4960..5628
                     /locus_tag="ACG8KQ_002371"
     CDS             4960..5628
                     /locus_tag="ACG8KQ_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9105277.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5695..6483)
                     /locus_tag="ACG8KQ_002372"
     CDS             complement(5695..6483)
                     /locus_tag="ACG8KQ_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9105278.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6566..8038)
                     /locus_tag="ACG8KQ_002373"
     CDS             complement(6566..8038)
                     /locus_tag="ACG8KQ_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9105279.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8168..9361)
                     /gene="metK"
                     /locus_tag="ACG8KQ_002374"
     CDS             complement(8168..9361)
                     /gene="metK"
                     /locus_tag="ACG8KQ_002374"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9105280.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9359..9438)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9627..9698)
                     /locus_tag="ACG8KQ_002375"
     tRNA            complement(9627..9698)
                     /locus_tag="ACG8KQ_002375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9663..9665),aa:Glu,seq:ttc)
     gene            complement(9710..9783)
                     /locus_tag="ACG8KQ_002376"
     tRNA            complement(9710..9783)
                     /locus_tag="ACG8KQ_002376"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9747..9749),aa:Ile,seq:gat)
     gene            complement(9796..9866)
                     /locus_tag="ACG8KQ_002377"
     tRNA            complement(9796..9866)
                     /locus_tag="ACG8KQ_002377"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9832..9834),aa:Gly,seq:tcc)
     gene            complement(9871..9943)
                     /locus_tag="ACG8KQ_002378"
     tRNA            complement(9871..9943)
                     /locus_tag="ACG8KQ_002378"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9908..9910),aa:Phe,seq:gaa)
     gene            complement(9947..10019)
                     /locus_tag="ACG8KQ_002379"
     tRNA            complement(9947..10019)
                     /locus_tag="ACG8KQ_002379"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9984..9986),aa:Asp,seq:gtc)
BASE COUNT         3237 a         2118 c         1661 g         3004 t
ORIGIN      
        1 aaaattataa tggaacagta acatttacaa aattagctaa agctgaacaa tatagacgat
       61 ctttagaatt gtcaaaagat taaatatata aaaagccttc gggctttttc ttttaacaat
      121 taataggaac gtatattcga aaggaataga aataatgtgg ttagaaaaaa taaatgacaa
      181 aaaatttaag tacaccgaac gttatacgga tcccttaact gaaaaaaaga gaaaagtgtc
      241 cgttacttta tctagcaatt cacgccaagc ttggaatcaa gctaacttac ttttgaatga
      301 aaaaatcgca gaaaaaataa aacgaaatga agaattacca ctaacttttg gagaattaaa
      361 aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa agttcctatc gaacaactca
      421 ggtttatcta tcattaattt caaaatatat aaaagatgat gtacttgtaa aaaacgttaa
      481 tagtaatctt atacaagata tgctagatta ctactatttt gaaaaaaatt tatcatataa
      541 ttatgtaata catttaaaaa cttttacagg aatgattttt aaattcgcca accgtcgata
      601 tggattaaaa tataatcccg taaatggggt ttctttagca agaaaaccta agacaaacga
      661 agaaataaaa aaagaaaaag atggttattt atcaaaagaa gaagttcaaa tgattgctag
      721 taaacaaaaa agttcacatc aacagtcacg ttattcttta attactaaat ttttgttttt
      781 aactggacta cgttatggtg aattgatatc tttgactgaa tctgattatg atggtaataa
      841 aattacggtt tctggtactt atgattacga gttaaaaatt aaaacaacaa ctaaaaatac
      901 aggttcttat agaaccattg aattatcaag taatgctaaa gaaatcatag atcaattaat
      961 tgaagaaaac aaattaatta agaataataa agataaatac attttcatct ctaaaaatgg
     1021 taacccgata tctattcaag catataatca atctttaaga gcagtatcca aagaattaaa
     1081 cctagataaa aaagtttctt ctcacatgtt aaggcattcc cacatttctc tacttacaga
     1141 attaggaatc ccattaaaag ccattatgga tcgtgtagga catgaagatt caaaaacaac
     1201 attaaagatt tatactcata caactaaaaa tatgcaaaat caactagtag aaaaactagg
     1261 aaaaatagaa atttaaattg ccccttttct gccccttata aaaacaaaca acataaaata
     1321 tataaaaaga accccgttat aacgaggttc ttttaattta actttcaaat acaaattgat
     1381 tatgataaag ttccgcataa aatccatttt ctgctaataa ctcgtgatga ctgccttctt
     1441 caatgacttc cccatcacgc aagacaacga ttctatctgc attcaagatt gttttcagcc
     1501 ggtgagcaat cacaaagctg gttcttcctt tgattgcttc atccattgct ttttggatcc
     1561 ttgcttctgt taccgtgtct acattacttg ttgcttcatc caaaatcaat aaagatggat
     1621 ttgtgatgat cgttcgagca atactgatca actgcttttg acctgtactg aataagttat
     1681 tttcttcgga tatctctgta tcataacctt tttctaacgt catgatgaag tcatgaatat
     1741 tcgcttgttt tgctgcattg atgacttctt catctgaagc gtccggtttg ccgaatgtga
     1801 tattgtcttt gatcgttccg gagaacaata cagattcttg caaaacgatt cctacatgtc
     1861 tacgtaatgc atcaagatca taatctcgga tatctattcc atcgatgact actgaaccat
     1921 cattgacatc gtaaaaacgg tttagcagat tcatgatcgt cgttttcccc gatcctgttg
     1981 gaccaacaag agcaaccatt tctcctttgt ccacattgat cgagacatct ttcaagacta
     2041 gtttttctgg agaataacca aaatctacgt gctgcaacgc aagacctttt tcaacaccat
     2101 caaatgcttt acagttttct ggtttgactt catctttttc atcaaatatt tcattcaatc
     2161 gtcttgcccc tgtaatcgct aattggatca tgctgtatcc agatgaaatc tgcatcagtg
     2221 gttggtaata ttgttgtgaa tactggacga atgtcacaac taagcctaat gccaccgaac
     2281 gttccaaatc accatttaat gctaaccagc cgccgaaaaa gataactatc gctgtattga
     2341 ccagtgacat tccttgcatc atcggaaata gtagaccaga ataagcttgt cctttgaagg
     2401 ttgcttcgcg aacactttga ttatgctcta agaatccttc aatcgtttct tcttgtaagc
     2461 catttgtgat gatgacacgt tgtccgctga ttttttcatc catataacca tttagtttcc
     2521 ccacttcatc ttgctgaaga tccacatatt tccgtgcttt gcggataatc aagacagcta
     2581 tcaaaacggc tactggagtg gaagcaattg tagcccaagc taactctaca ttttggcgga
     2641 acatcatgat caagatcccg acaagtaaaa cagcattcgt gatgacttga agcaaagctt
     2701 ggttcaaact attttgaata ttatctaagt cactggtaaa acgacttaaa atttcaccgt
     2761 cttgatgaga atcaaagaaa cggatcgtca atttttccaa tttgttgaat agaccgatac
     2821 gcattcggtt cgttgacttc cctactactt gtgtgaacaa aatactatag ataaagttgg
     2881 ctgcactagc tagtacataa aacagcaaga gtttccatat tacagaaaca aatttgcttt
     2941 tatcgtctac gccttgcatc aagcctccta cgtaattagc taattcttga aaagcttccc
     3001 cgatgtattg aggtgccttt acttgtagat aggtagcaat gatcactgtg ataaagataa
     3061 agaaaaagga gagtttgtac cgcttcaaat agtgatagaa aaatttactt gcttttatta
     3121 gatcagtcat acttactcct ccgttccttt ttgagtttca aagatttcct gatagacacg
     3181 gtttccagct accaattcct catgggttcc ttcgccgact aatctgcctt catccaatac
     3241 taagatgcgg tctgctttga caacagaagc aattttttgt gcgatgacaa tcgtcgttgt
     3301 tcccttcaaa tctcgatcga gtgcttctcg gaccagcttt tcagagcgag catccagtgc
     3361 actcgtactg tcatctagga tcaagatctt tggattaccg ataacgccac gagtaatgga
     3421 taaacgttgc ttttgaccac cagaaaagtt actgctacgt tcttctattg gtgcttcgta
     3481 acgatcagat aacttttcga tgaattcttt tgcttgagca atagaagaag catgttccat
     3541 ctctttttct gttgcattct ttttcccttg gcgcaagttt tgagcaatcg ttcctgagaa
     3601 taaaattgct ttttgcaaaa caaaggaaac cgtttgtctt aaactctttt catttacttg
     3661 acgaagatct acaccaccta cttcaacttt gccttctgtc ggatcaaata gacgagggat
     3721 caattgtgct agtgttgact tcccggcacc agtcgccccg acgataccaa ccatttctcc
     3781 aggtttgata gaaaagctga tatctttaag cgtatctgta tcctcaccag gataacgaaa
     3841 agaaacatgg tcaaaagtga cagaacccgt caactcttgt tctggaacgt ctaaataagt
     3901 aagatctggt tcagcatcta aaatttccgc aatccgcttc agcgatacgg ctgctcttga
     3961 agtcatcatc atcatcattc cgccaataat gattgccatc ataatctgca tcaagtagtt
     4021 catgaatgaa gcaattccac caattaacgt aggatcatct ttaacaagat cgctcacaaa
     4081 aaagatcgcc ccaaccacag ccaaattggc agccagcatg aaggacggga tcatcacaga
     4141 aaataatgta ccgactatga tgttgtgttt tgttaagtcc tcacttactt tgctgaatga
     4201 cttcaattgg ttttcttctt gtacaaaaga tttcactaca cgaatcccta gtaagttttc
     4261 ttttgcaatg ctattcacac gatcaatcag tttttgaatg atcatgaagt gcttgcccat
     4321 ccttgtaaat gaaagtgccg taatcagaaa taccgtaacg ataagcgcta cgatgatcca
     4381 ccataattgc ggcatcgtga tcatagctaa aatgaaggca ccaataaata agaatggaat
     4441 tcgaaaaagt gattgcaaag aaatcatcac taaattttgg atttgcgtga tatcatttgt
     4501 tagacgaaca acaagatttc cagcagagaa ctgttcgata tttccaaatg aaaatgtctg
     4561 gatctttcga aatgcttctt ctcggatatc tgcacttacg ccttgagcta cttttgcaga
     4621 gaaaatcgtg ttcgttacac ctgcggctaa tcctagcaaa gccagactga tcaggtaaat
     4681 accaattgtt ttcatttcat cattatcttc tgtaataata gcttccaata cttgctgtag
     4741 taacttaggt tgccataatg ctgctgcaac catgactgct acggacagca aggaaataaa
     4801 aacagctact ttatattttt tcgcatgtcg taagattaat ttcattcgtt gattacaccc
     4861 ctttgtttaa attgacagga ctaatttaac actgtaaaat tagaccgact aacaatttac
     4921 catgtttaaa aagataatgc aatacacgag gttgataaaa tggaaataaa gctttctcaa
     4981 gcaatcatca ttcaggccgc ctttgatatc ctggctgaaa cacaagctct ttcgcagctt
     5041 tccatgcgaa acatcgcaaa cagaattcat gttcaagcac ctgctttata ttggtatttc
     5101 aaaaacaaac aacagctctt gcaaataatg gctgaaacaa tggaagaaga attagcgctt
     5161 ccagatccaa ctttgccttg gaatgaacag ttgctgcagt atatggaaaa ttactatgac
     5221 ctttatacac gtttcccttg tggggcagaa ttagaaatca atactgtgcc cgcttttccg
     5281 agtcgattag aacatttgga gcaaatgatc caattacttg ttcgtgaagg attttcagtc
     5341 acccaaagtc atcaggccat tgcctctctc cacaatttat tgattggaca gttaatggat
     5401 cagcagcatg aacagcagct tcgacaaaaa attatggacg gaaatgaatt tttgaaagaa
     5461 acagttctat ttatgcgtaa ttatgtagtt gaaaatcaat tagagggatt taaagaaagt
     5521 atgcagtccc atgcagaatt gaatgaaaaa gcactctttc taacgagtgc acaaacctat
     5581 attgaaggct tatgccaaca aaataaaaga agagaaaacg agcagtaaaa aagctcctat
     5641 agagatcact atgtttgtga tctctatagg agctatcttt ttaagactct ttttttattt
     5701 ttcttccatt agccacgcca atgcacgttg aatccattgg tcccagaaga tccagttatg
     5761 atcgccaggt ccttgttcaa aggtgaccgc caagccttgt tcctgcatag cagataccat
     5821 ttcctgattt gcctgataaa gaaaatcaga ttccccacaa gccatataaa atcttggaac
     5881 tggttttccg ctttttacta gttctttcaa taagtattgc ggatcatttt ttgaaccttc
     5941 gatttgatcc aatggtccaa agatcccttc ccaaaaagat gctttacgaa ccatcaaaag
     6001 atcatccatt cgatttccta cagaaacagc gcctgaaaga gagattactg ctgcgaaatt
     6061 ttctgatttt gcaaggccta gttttaacgc gccatatccg cccatcgata atccagcggc
     6121 atatgttttt tctctttttc ttgttaattg cgggaataat tcatgacaaa tctcaggtag
     6181 ctcttctgca atgaatgtcc aatacttcat gtcataaact gtatctgtgt accagccaag
     6241 atcagtagat ggcatgatga cagctaatcc gtagtttgct acataacgtt caatcgacgt
     6301 acgtctttcc catacactat gattgccacc cattccatga agtaaatata aaacaggtat
     6361 atccgttgtc tcttgctctg aactggtgcc aatcaccttt tttgttgttt gcggtagtat
     6421 cacatttaga ttgacttcca tttctaacac attcgaataa atatttgctt gaataaatgc
     6481 catattttcg ccttccttag taagaagtta tttttgaata actttaaatt tgaatgattc
     6541 ggctacgtac atatcttaac cgaaactact gttttatttt accacttttt tcatgaacga
     6601 aaagcgttaa gaccaaagca atgatcacga gtaacaagcc aactagataa acggaatgta
     6661 acccatcata aagaattttt cttaatggta acaaaatatc cgcatcaata tatttagccg
     6721 tttgtggatt caccagttta ttcatgatat ctgcatcatt tgttaaattt gctgctgcaa
     6781 gttctcgttt agttacccca tttatcaata aaccaaaaat agaaaccatg actgtttgac
     6841 caattgttcg aactaacgta ttaaaagaag ttgctacacc aagctggtca cgagatacac
     6901 tattttgtgc actgacagtc gaggtagtca tggtcacacc taagcctata ccgatgaccg
     6961 ttgatatagc aaaaaaccat aaatagttac ttctgaaagg cataagatac aaccaagcgc
     7021 ccccaatagc tgcgattagt agtccaattc tcaagaccca atgagttttc atttgagtca
     7081 tccacttgct tgcaaggaaa gaaccgacca tccagactag tgataatggt gctaatacca
     7141 gcccaccaat tgctgcattc agccctaata caccttgcat ccacataggg atataaacat
     7201 cgattcccat caaaaaacca ctagccaatg ctgcagccaa gttgaccaca acaaatagtg
     7261 gatttttgaa taaatgcatc atgatgacag gatctttagc tcttttttcg gcaaaaacaa
     7321 aaatacctaa cattacgata ctacctagca gcaaagcaga aacaagcgga ctcattcctt
     7381 cgtctcctaa taattggaac cctaacagca atgagacaag catggccatc aaactaaaac
     7441 taccaaaaat atccattggt ttagactcat gtatgatttt tggttcattt aaatatagcc
     7501 aaatcaaaat aattaataga attccgatcg gcacattgat aaaaaagatc cagtgccagc
     7561 tgatcgtttc aacgatgaat cctcccgcaa gcggtccaaa aacactggca atcccccagg
     7621 ctgaactatt caaacctaaa acttttgctc gcttttcaat agaataaaga tcgccgatga
     7681 tcgtcaaagc tactggcatc attgccccag cacccatccc ttgaatcgca cgagcagcaa
     7741 tcaacccaat catgctttga gataaaccgc aaaataacga accgataata aacaataatg
     7801 tcccaaaaat aaagattggt ttccgtccca ctttatccgc aagttttccg taaatcggag
     7861 taagcatagc gtttgtcagc aaataaatcg aaaataccca gttcatgatt tccattccat
     7921 gtaatgaacc gacaatcgtt ggcatagcgg ttgttacgat tgtaccttca atagccgtca
     7981 tgaacgttgc aatgaaaata ctcactgtga ccaaagtaac atttgtttct tttttcatct
     8041 tattcctccc tttcattatg acaggatcta aaaatagata aataattact atctttttca
     8101 agaggttaac tgccataaaa aaagctggaa caaccaccac ttggcgatcg ttccagctga
     8161 atataactta ttttgctaaa cttgctttca acgcatctac tttgtccgta tgttcccaag
     8221 gcaaatctac atctgtacga ccaaaatgac cgtaagcagc tgtttgtttg taaatcggac
     8281 gacgcaaatc aagcatctcg atgattcctg ctggccgaag atcgaagttt tctctcactg
     8341 cagcaatcag ttcttcttcc ggaaccgttc cagtaccgaa agtattgatt gagatcgaaa
     8401 caggttgagc aacaccaatc gcataagcta gctgtacttc tgctttgcga gcaagccctg
     8461 ctgcaacaat gtttttagca atataacgtg cagcatagct ggcagaacgg tcaacttttg
     8521 tcgcatcttt accagaaaaa gcaccgccac catgacgagc ataaccgcca tacgtatcaa
     8581 caatgatttt tcttcctgtt aatccggcat ccccttgagg accaccaatg acaaatcggc
     8641 cagtcggatt gatataatat ttcgtttgat catctaataa ttcagctgga ataacttcct
     8701 tcactacttt ttcgatgaca tcatgacgaa ttgtttcatt atctactgca tcatcatgct
     8761 gtgttgaaat aacgattgta tctacgcgtt ccggttgtcc ttgatcatca tattcaaccg
     8821 ttacttgaga ttttgcatct ggacgtaaat acgttaattc atttgattta cgcaaatctg
     8881 ccaagcgtcg taccagacga tgactcaaag cgattggtaa aggcatcaat tctggtgttt
     8941 catctacagc aaacccaaac attaaacctt ggtcaccagc accaatctca tctaatacgt
     9001 cttttttatc ttcatcccgg atctcaagtg cttcatcaac tccttgagca atatcaggag
     9061 attgttcatc aatcgcaacc aatacagctg ctgtatctcc atcaaatccg aattttgcac
     9121 gtgtataacc aatttctttt attgtttccc gtacgacttt ttgaatatct acatatgccg
     9181 tcgtagatat ttctccaaat actaaaacaa gaccagttgt tacagacgtt tcacacgcta
     9241 ctcgtgctgt tggatcttgt tttaaaattg catccaagat tgcatcactg atttggtcag
     9301 caattttatc tggatgtcct tcagaaacag attctgatgt aaataagtgt ctttctacca
     9361 ttataatgat tccccctagt taaattcggt tacaaggcat ctccgtcctg cggatccttt
     9421 cgggaacttg caacgaacca agtatagcag ataaaacagt tttctgctcg tattttttca
     9481 tttttttgct taaaaaatat aaaaatattt tacattagaa ctgtccaaaa aagcttagca
     9541 caatcatcca taaagataac tctgctaagc tttttaaaat cattctttca gataaaaaga
     9601 aaaagatact caaaaagtat cttccatgac ccgtacggga ttcgaacccg tgttaccgcc
     9661 gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccaa taaaataaat gggcctaaat
     9721 ggactcgaac catcgacctc acgcttatca ggcgtgcgct ctaaccagct gagctatagg
     9781 cccataaaaa ctaaaagcgg gtgacgagaa tcgaactcgc gacaacagct tggaaggctg
     9841 tggttttacc actaaactac acccgcataa tggcgcggga cagaatcgaa ctgccgacac
     9901 atggagcttc aatccattgc tctaccaact gagctaccga gccaaacggt tccgacggga
     9961 atcgaacccg cgatctcctg cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKCE010000107         9813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000107 DAZKCE010000000
VERSION     DAZKCE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.108"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8KQ_002380"
     CDS             complement(34..2154)
                     /locus_tag="ACG8KQ_002380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9105281.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8KQ_002381"
     CDS             2384..3562
                     /locus_tag="ACG8KQ_002381"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9105282.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8KQ_002382"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8KQ_002382"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9105283.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8KQ_002383"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8KQ_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9105284.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8KQ_002384"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8KQ_002384"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9105285.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8KQ_002385"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8KQ_002385"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9105286.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8KQ_002386"
     CDS             7746..9092
                     /locus_tag="ACG8KQ_002386"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9105287.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8KQ_002387"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8KQ_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9105288.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3129 a         1782 c         2009 g         2893 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DAZKCE010000108         9768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000108 DAZKCE010000000
VERSION     DAZKCE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.109"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..521
                     /locus_tag="ACG8KQ_002388"
     CDS             282..521
                     /locus_tag="ACG8KQ_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105289.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="ACG8KQ_002389"
     CDS             complement(883..1143)
                     /locus_tag="ACG8KQ_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105290.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="ACG8KQ_002390"
     CDS             complement(1328..1825)
                     /locus_tag="ACG8KQ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP9105291.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACG8KQ_002391"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACG8KQ_002391"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP9105292.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACG8KQ_002392"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACG8KQ_002392"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP9105293.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLGIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="ACG8KQ_002393"
     CDS             complement(4547..5209)
                     /locus_tag="ACG8KQ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9105294.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="ACG8KQ_002394"
     CDS             complement(5219..6034)
                     /locus_tag="ACG8KQ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9105295.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="ACG8KQ_002395"
     CDS             complement(6296..6739)
                     /locus_tag="ACG8KQ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP9105296.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="ACG8KQ_002396"
     CDS             complement(6873..7211)
                     /locus_tag="ACG8KQ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9105297.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="ACG8KQ_002397"
     CDS             complement(7199..7576)
                     /locus_tag="ACG8KQ_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9105298.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="ACG8KQ_002398"
     CDS             7800..8993
                     /locus_tag="ACG8KQ_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9105299.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="ACG8KQ_002399"
     CDS             complement(9159..9581)
                     /locus_tag="ACG8KQ_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105300.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2922 a         1942 c         1594 g         3310 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg cctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataa
//
LOCUS       DAZKCE010000109         9593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000109 DAZKCE010000000
VERSION     DAZKCE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.110"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG8KQ_002400"
     CDS             complement(<1..641)
                     /locus_tag="ACG8KQ_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9105301.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG8KQ_002401"
     CDS             complement(643..1407)
                     /locus_tag="ACG8KQ_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9105302.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG8KQ_002402"
     CDS             complement(1415..2092)
                     /locus_tag="ACG8KQ_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9105303.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG8KQ_002403"
     CDS             complement(2129..3514)
                     /locus_tag="ACG8KQ_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9105304.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG8KQ_002404"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8KQ_002404"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8KQ_002405"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8KQ_002405"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9105305.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG8KQ_002406"
     CDS             4453..5310
                     /locus_tag="ACG8KQ_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105306.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG8KQ_002407"
     CDS             complement(5391..5726)
                     /locus_tag="ACG8KQ_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105307.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG8KQ_002408"
     CDS             complement(5793..7163)
                     /locus_tag="ACG8KQ_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9105308.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG8KQ_002409"
     CDS             complement(7241..7777)
                     /locus_tag="ACG8KQ_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9105309.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG8KQ_002410"
     CDS             8086..9567
                     /locus_tag="ACG8KQ_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105310.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAZKCE010000110         9466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000110 DAZKCE010000000
VERSION     DAZKCE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.111"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..2440
                     /gene="leuS"
                     /locus_tag="ACG8KQ_002411"
     CDS             26..2440
                     /gene="leuS"
                     /locus_tag="ACG8KQ_002411"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9105311.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2788..4341
                     /locus_tag="ACG8KQ_002412"
     CDS             2788..4341
                     /locus_tag="ACG8KQ_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9105312.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4722..6506)
                     /locus_tag="ACG8KQ_002413"
     CDS             complement(4722..6506)
                     /locus_tag="ACG8KQ_002413"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105313.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6521..8242)
                     /locus_tag="ACG8KQ_002414"
     CDS             complement(6521..8242)
                     /locus_tag="ACG8KQ_002414"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105314.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8342..9103
                     /locus_tag="ACG8KQ_002415"
     CDS             8342..9103
                     /locus_tag="ACG8KQ_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9105315.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3062 a         1692 c         1908 g         2804 t
ORIGIN      
        1 tttcaataga acaggaggac agcaaatgag ttacaatcat aaagaaattg agaaaaaatg
       61 gcaaaaatac tgggcaaaaa cgaatacttt caacacccat gatgatcctg aaaagccaaa
      121 attttacgca ctggatatgt ttccctatcc atccggtcaa gggctacatg tagggcatcc
      181 tgaaggttat acggcaacgg atatcctttc acgtgtgaaa agaagtcaag gatacaacgt
      241 gctgcatcca atggggtggg atgcattcgg tctgcctgct gaacaatatg cgctagatac
      301 aggaaacgat ccagctgagt ttacccaaaa aaatatcgaa acattccgac gccaaatcaa
      361 ttctttagga tttagttatg actggaatcg cgaagtgaat acaactgatc cggagtatta
      421 taaatggaca caatggatct ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga
      481 agtagcggta aactgggtac ctgaattagg aacagttatt tcaaatgaag aagtgatcga
      541 tgggaaaagt gaacgtggtg gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct
      601 gaaaatcact gcttatgctg atcgcttatt ggatgatcta gaattagttg attggccaga
      661 aagcattaaa gaaatgcaaa gaaattggat cggtcgttca gtaggagcaa atattgaatt
      721 taaagtggct ggtacggata aatcctatac cgtatttacg acacgaccag atactctatt
      781 cggtgcaact tactcggttc ttgcacctga actagatctt gttagagaaa ttacgacccc
      841 tgaacaaaaa gctgctgtag aagcttatat cgaagagaca gctaaaaaat cagatctaaa
      901 gagaacggat cttgcaaaag aaaaaacagg agtatttact ggtgcttatg caatcaatcc
      961 agttaatggc aaggaaatcc caatctggat cgccgattat gtattatctt catacggcac
     1021 aggagccatc atggctgttc cagcacatga tgaacgtgac tttgaattcg ctcaaacttt
     1081 cggactagaa attcttccag ttatcgaagg tggagatgtt gaaaaagctg cttacacaga
     1141 agatggtacg cacatcaact ctgaatttct agacggcatg aacaaacaag aagcaatcga
     1201 taaaatgaat gaatggctag aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt
     1261 acgtgactgg ctattctccc gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg
     1321 ggaggacgga acagtcacag ctgtcccaga agaagagcta ccattacggt tgcctaagac
     1381 aaaaaacatc aagccaagcg gtactggaga atctccatta gcaaatatcg aggagtgggt
     1441 caatgtcgtt gatcctgtaa caggtaaaaa aggccgtcgc gaaacgaata caatgccaca
     1501 atgggcagga agctcatggt attacctgcg ttatatcgac cctcataaca aaaaagaact
     1561 agcagattat gaaaagttga agcgttggct accagttgac atctacattg gtggagcaga
     1621 acatgcggta cttcatttac tatatgcacg attctggcat aaattcttat acgatatcgg
     1681 tgttgttcct acaaaagaac cattccaaaa actatacaat caaggaatga tcttaggcga
     1741 aaacaacgaa aaaatgtcaa aatctcgtgg taatgtagtc aatcctgatg atgtggtcga
     1801 aacttacggt gcagatacac tgcgcatgta cgaaatgttc atggggccat tagatgcttc
     1861 gattgcttgg agcgaaaatg gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg
     1921 tttgatcgtt gatgagaaca ataaaatgcg tgaccgtatc actacattga atgatggcaa
     1981 gcttgataaa gtgtatcatc aaacagtgaa aaaagtgaca gaagactatg aaaatcttca
     2041 tttcaataca gcaatctccc aattgatggt ctttatcaat gaagcctata aagtggacgc
     2101 tctgccttat gaatatatcg aaggatttgt tcaactactc gcaccgattg ctcctcacat
     2161 gggtgaagaa ctatgggcaa tccttggaaa tgatggtggt atttcttacg caccatggcc
     2221 aacatacgat gaagcagcac ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg
     2281 taaagttcgt gcgaaaagca atgttccccg agacttagga aaagatgagc tagaaaaagc
     2341 agcattagcc aatgaaatag tgcaagaata tattgaaggc aaaacagtca gaaaagttat
     2401 tgcggtacca aacaagttag tcaatatcgt tgctaattaa ttggctcttt gtcaataagg
     2461 actgatgagt tgtgcaaaat caaatctggg ccagaatgaa cttcattctg tctcagattt
     2521 tttgttattt caaattgatt aattgattga tcaaaaaggt tttgattttc atgttctcaa
     2581 acacgttcaa tgtctttttc ttctgaatgg tcttgtaaga gatctctgta atcatcaaat
     2641 ttttgtctat taatcttagt attttttgat gtataaacta cctgaaattg agagtacata
     2701 ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg acgcataatg
     2761 aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat cgaagtatta
     2821 tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag
     2881 cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc gttcctatat
     2941 cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa taatttgtat
     3001 cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt tcttctactt
     3061 atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag tcaaagagca
     3121 caacagtttt tacataataa accaacttct caagatgatg actatcttta ttttgagaag
     3181 acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt gctgagatca
     3241 cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat gaacacattc
     3301 catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga
     3361 tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct tttagctgga
     3421 acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa tgctgcttct
     3481 cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga agggttgtta
     3541 gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct caaacttgat
     3601 acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt atttgtgccg
     3661 attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct gattcctgaa
     3721 ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt tatctattct
     3781 tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg gcaaacgtct
     3841 attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta tggcaacgat
     3901 ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta catttacact
     3961 actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa tcagccagta
     4021 caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga aagtttgttt
     4081 tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat
     4141 cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat
     4201 acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt
     4261 gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca gcaactatcg
     4321 caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag caccgagtca
     4381 caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac tcgtgattcg
     4441 gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt ttacaagatc
     4501 gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa gggattgtga
     4561 caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa agtatcatta
     4621 gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct atcgatcatg
     4681 gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat ctgaaattga
     4741 ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg tgttcctttt
     4801 tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat cgttgacaaa
     4861 cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc ttgctgaatc
     4921 aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac gacaggagga
     4981 ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga taacgtgccg
     5041 ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa gtgatcacat
     5101 tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt cccgtaggcg
     5161 atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa
     5221 cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc gatctttcct
     5281 ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc tgcaccagtt
     5341 ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg attcatcaat
     5401 attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc tttcggttgt
     5461 tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa tatctcaaat
     5521 atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga agcggtagaa
     5581 attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc gatcgtcaca
     5641 ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc gagttgatta
     5701 atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt aaaagcgttg
     5761 tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg attgaatgtt
     5821 ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac acccagttca
     5881 tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa
     5941 ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata aaacagcatc
     6001 agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc tgtcaataac
     6061 acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc tacttgatga
     6121 ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt tcgcaccctc
     6181 aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga agtgatgctg
     6241 cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg tttgatatca
     6301 ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc atcgatcccg
     6361 attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact gcatagaaac
     6421 aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat caaagcacgc
     6481 gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct tgtgattttg
     6541 caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct tgtccaacga
     6601 tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg aggcgttgag
     6661 caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt aatgctgcat
     6721 ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct gctggtttga
     6781 tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta gaaccgcctt
     6841 gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct aattgagcga
     6901 tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc tgcaagttgc
     6961 ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg tgggagcgga
     7021 tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt cctatcgtag
     7081 ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg gtcccgccaa
     7141 cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga actggatcag
     7201 agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg
     7261 ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct tgcagtattt
     7321 cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc atagctgcga
     7381 tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg acaccgattt
     7441 gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca taaacgattc
     7501 ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact ttgatcattt
     7561 gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat tgattatcaa
     7621 aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg atccgatcca
     7681 tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga taaacaatcc
     7741 atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa taacgaaaca
     7801 tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg caaaataaaa
     7861 caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga gatccagaac
     7921 taaaacgact gatatccttc attgacagct gttggaattt acggaaatat ttctcccgcg
     7981 tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca actgcagcga
     8041 ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata actgaggctt
     8101 cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca cctaatgtac
     8161 cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg tttttctgta
     8221 agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt tcgattctat
     8281 accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa
     8341 aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc caaaatcaac
     8401 gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag agaatgatta
     8461 tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt atagaaaatt
     8521 agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg agttatatga
     8581 gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga aacaaaaaca
     8641 aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa acgaggaaga
     8701 atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg aagatattgt
     8761 ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta tgccagcttc
     8821 cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt tatttactca
     8881 agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat tgaaatccga
     8941 gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt atgaaaaagg
     9001 atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg aaaacaatat
     9061 cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat gtgtttaaga
     9121 taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc gattacgggt
     9181 ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag aggaaatatg
     9241 tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt gtcctcttaa
     9301 aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga actgagacta
     9361 atgagaagaa aaattcatat tgttatgagt gagattgtat cagatgagaa tgataaatta
     9421 aataggtgca taaaagcatt acacttaaaa tacattgttg cttaca
//
LOCUS       DAZKCE010000111         9385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000111 DAZKCE010000000
VERSION     DAZKCE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.112"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..2078
                     /locus_tag="ACG8KQ_002416"
     CDS             21..2078
                     /locus_tag="ACG8KQ_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFP9105316.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2116..4215)
                     /locus_tag="ACG8KQ_002417"
     CDS             complement(2116..4215)
                     /locus_tag="ACG8KQ_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFP9105317.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(4258..4506)
                     /locus_tag="ACG8KQ_002418"
     CDS             complement(4258..4506)
                     /locus_tag="ACG8KQ_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105318.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            complement(4516..5340)
                     /locus_tag="ACG8KQ_002419"
     CDS             complement(4516..5340)
                     /locus_tag="ACG8KQ_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9105319.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(5337..6092)
                     /locus_tag="ACG8KQ_002420"
     CDS             complement(5337..6092)
                     /locus_tag="ACG8KQ_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9105320.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(6105..8081)
                     /locus_tag="ACG8KQ_002421"
     CDS             complement(6105..8081)
                     /locus_tag="ACG8KQ_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9105321.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(8134..8805)
                     /locus_tag="ACG8KQ_002422"
     CDS             complement(8134..8805)
                     /locus_tag="ACG8KQ_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9105322.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(8865..9074)
                     /locus_tag="ACG8KQ_002423"
     CDS             complement(8865..9074)
                     /locus_tag="ACG8KQ_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105323.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(9141..9380)
                     /locus_tag="ACG8KQ_002424"
     CDS             complement(9141..9380)
                     /locus_tag="ACG8KQ_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105324.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2759 a         1879 c         1484 g         3263 t
ORIGIN      
        1 taaatttagg agttgaaaaa atgaaattat ttaatccaaa aactgtttta gctattggaa
       61 catttgctgc ctcacttagt cttgctggat ctgttgacgc tgcttcaact aacgtcatgt
      121 atcgccttta caatccaaat aataatgaac atttttacac ctctagcaaa aaagaacgtg
      181 accatctagt aaagataaaa tggggaaatt atgaaggccc cgcctgggaa gcaccgacta
      241 gcggtggcca tcttgtctat cgtttgtaca ataaaggtct tcgtgatcac cattacacag
      301 ctagttggga tgaagtgaaa tggttgacta aaaattacgg ctggacttat gaagggcctg
      361 cctggcgttc tgccgaaaaa aataataaac cgatctaccg ccttttcctt cctggtgtaa
      421 aatctggctc acatcactac actgcaagtt ggaaagaagt acaatggtta accaaagact
      481 atggctggaa atacgaaggt atcggttggt atggcgctga cacctctaaa cccaaagtga
      541 ataaatcggc tctagaagct ctttatacga gcgttaaggg aacgaaaaaa ggtgactata
      601 ccgacaatac ctggaacgca ttccaaaccg ctttaaacaa tgctaagaaa gtgttaaatg
      661 actcaaaagc gactcaaaag caagtggata gtgccaaaaa taaattagat tctgcttaca
      721 aaggattaaa gaaaaaacca gttcctactg tgaataaagc tgaattacaa gctttataca
      781 accgagtaaa atcaactgct aaaggagatt ttacagaaga atcttggaat aattttcaga
      841 ctgctttatc caatgctaaa aaagtactag acgattctaa ggtttctcaa acacaagtaa
      901 acaatgctaa aaatagtctt gatgctgcat ttaaagggtt gcaacacaaa cctgaaacac
      961 caaaagaata tacggttact attaaccata ttgacaatca aacaggtcaa gtgattgcta
     1021 cagatcaagc aaaagtaact tctggttcta cttacactgc taaggcaaaa gcatttaaat
     1081 atagcgaagg cgctaaagat aattttagct acaaagtgat tggtaatgat actcaaagca
     1141 aaacaatcac aggtaataca acaatcacat tcaattataa tcaagtgcat caaatcaatt
     1201 tgttgtgcaa taacaactac aaaaacgaag gacgagttaa tattttgaaa gaacagatag
     1261 tcaatgtcac tcacggtgac agtgtcactt taactgctcc ttctattcaa ggatatgttt
     1321 tagacgatcg ggtagaacca acaaattcag taaccttaaa caatgtgact gaaagtcaaa
     1381 attatatttt caactacact cgtaaattta atgtgactgt taatcatgta aatgctgata
     1441 ccaacgctgt tctctctagt gaatcaaaaa cagtatacga aggggatagc tttgctactc
     1501 cttggaaaaa catgactgat caaaattatt tcttgtgcag aaatgacgat cctaccgtta
     1561 ccgtggataa aaacggccaa cgatccatta ataacgtcga tcaaaataaa acaatcacat
     1621 tcaaatataa aaagatttca ttagacgaat tacgtaactt tatgcgtcaa aaagaacttg
     1681 cttggttaaa tgattatcgc caacaaaatg gtgttgcccc aatgcaattc aatgatattg
     1741 ttcaacaggc tgcagatatt cgagctaaag aattacaagt cagcttcagt cactatcgcc
     1801 ctggtggtgg tactttccaa gatttattgg aaagcttagg ctgctacggt gctaagggag
     1861 aaaacataag tcaaactggg ctgtatattg atgacctttt atcagacgat ggtgctatcg
     1921 atgccatggt aggatggaaa aatagccctg cccataatgc aaatttactt tataataacc
     1981 aaagtattct cggattaggt cataattttg tagttgactc tgctggtaga attggcgtag
     2041 ataatgtatt tattagtgcg aatccaatct tcaaataaaa gaaaacaagg ctgattctca
     2101 gccttgtttt tatatttatt tgagttttaa aactacatag cccaatgatg ctaaagtaag
     2161 aaataatata cttccaataa cagttagcac taaatgattc gatccagttt ttggcacaat
     2221 gatctgaggt aagttaggtt ttaccccaat cgttactaca ttagttgctt tctcaatact
     2281 ttctactgaa acttttgctt ggttgtcgat atccaattct tcattcaaag gagttttagc
     2341 tgtaaatatc agctgaattt ctcgtccatc taaaagattt ttacttaaga aatcagcgga
     2401 agcttcccac ttcagcgttc ttgaattgct atcataggtt agtgtacctt ctgaagtaat
     2461 atcttttcct gtactcatat ctatcactcg cgcagaacct tctttatacg tcaaacgatc
     2521 ttccactgga tcttgaatac taaattgctt gtaatgatct gaggcaatgt ctacaccttt
     2581 tgtatgccat ttttgtgtga ctagatattg tacttcagat cctgcattta aaggagctgt
     2641 aggcgttcct tttttaccat catttcgacc atcaacatct ttcccatctt gttccacttt
     2701 tttatttggt ttaagagaga aatagttttc tactgtattt gttttttgtg ttcctttgtt
     2761 cggatcattg ttaaacgtta cagtagccac atttggtgca attccttttg catcattttt
     2821 tgctctcata tggagctgca aaatataatc cctaccgtaa tttgcagcgt tagctaaaaa
     2881 gctgtcagaa gcagtataag tgactgtatt acctgataca gttaactttc ctcctgtatc
     2941 agcttttttc caagtaccat tcgcttcaac ataccacaat gcgccatcat agtttttccc
     3001 atccgatacc atatcaaaat ttttgctgtc aaaggtatca gtgattttaa atgatttgta
     3061 tttgttcatt gtgttgactg ttaatgcatc aaacttttgg tgaatatgat agtaaacatc
     3121 tgatccttgt ggaatcactt ttttgtcaat attgtgtgct gcctcttcag tattagcttt
     3181 gttaaacact tcttttgtag gcttcgatgg ttgtggtgtc catagcaatg cagtatcaaa
     3241 gctaaacatc atactattcg tatttgcgcc aatcatgaat gttggggaag cgccttgaag
     3301 cgtatacgtt acaccacttc tgtgataagt atcagctcct acaacatcgg tgaaatgatc
     3361 attcgagcta taagcctttt ctgcacgtcc tcccactact tttgaagtta aattgtttgt
     3421 tgataacgca ggagctttgt attctgcaca tgctgtatct gttgtgacaa aataaggtaa
     3481 gctacccatt ttttcatacc caacaaattc tctcatatca atattacgtt caccgacaga
     3541 ttcattattg tcattatacc aagacacacg ttgctgatac tgtccattta aactgtaaat
     3601 ggatagaaat gtatcgttta aatcaatacc agctgctctc atcgatctca tttttgattg
     3661 aatttctggc tgtggttcac tcaaagctac ttttgttttt gttcctgcat acttaaattc
     3721 tagttttgca ttcatgacat tgattgactg catgaaaaat cctcgataaa tatttttatc
     3781 aaagagcaaa taattataat actttttgcc attaattgtt ttagctaatc cgtcttggtt
     3841 aaccccagcg tgcgctacaa aattagaaaa agtcacgtca caatcaattt cttttcctag
     3901 ataagtacct actttttgat aatgggcaac gatttttgac gtacttttca aatctttgac
     3961 ttttggccaa gcaagtactt tttgtttttt agttgaatac tcatctagta tcttaaatcc
     4021 attgatccca gttacttcga tatttctcaa tgaatcacta tcatgataaa gcaccagatt
     4081 attattttct ttgatttgac tcggatcaac tttttctaag gcctggactg cagttcccct
     4141 cgcaaaaaga agaaggaaca aaaacaacac taaagtaatt actcttcccg tagtgcaatg
     4201 aattttattt tccataatgc cctccatcta atattttttt aaacgatcgt aagcttttca
     4261 ttcttttttt cctaataccc acgttaccaa tcctgtaaga agaaccaaac taaccataaa
     4321 aattggattg atcaagagct ttttaatcac ctctaatggc atggaagcac attgaatgtt
     4381 tagaaaatga atgaacaatc gttgatatct tggataaatt gataattgag aaaaaattaa
     4441 accgcaacac aatattgttg cggctaaaaa ggatagatta aaaactaata ttcttattat
     4501 tttcatcatt tcatcctaat tatcaaaaaa taattgagca aaaaattccc aacaattttc
     4561 aaaaaagttc ttctttacac cagttgacac aactttttta atttcttctt tcggctctga
     4621 tttctctgat ttagggtata accaaacatc cgtcaaaaat tcaccttttt catttgaaac
     4681 aggtgtcaat aaaacaatat cttcactgtt tgaaatgttt tcagggcttg attcattaac
     4741 gatatagtat gcttgttttt tgtttgcagg tacttcaatg gaaaattcaa tcacgccttc
     4801 ctgatcatca atcgttttgg tttctccttc agccactttt ttcaactggt cgtatgaaag
     4861 tttttttgct cgatctttca attttgactc aattatttct acagattgat ttttctcatt
     4921 atcatcttgt gacatttctt ctaagatatt cgtgatatcg tagacaataa acttcgcgcc
     4981 attgatacct tcttgctggg ctacttgact gccttgattg ttgatttgat tcgaagaaat
     5041 aattttttga atatgaatgg cttgcttgat cacttgcgta tcttctacgt cttgcggctt
     5101 ttctgaagtc tctgaagaac tttctgacgt tggattcgtt gattcagtaa agcttatatt
     5161 tgaatctgcc gtttcttctg tagtattctc cgaactgtcc gtttcgcttg gtgcagtaat
     5221 tgaattatct attgtttgag tggtactact atcactgctg tcagcgaaag acagttgcga
     5281 ataagctaca gtagaaaaga gagctgctac acaaacaaac agccattttc tcttattcat
     5341 tccgatctcc tccttatttt tttataaata aaaataatag gaacgcaaaa aaaagcgaat
     5401 agcagtgcta atcgccaatc caatgaaatt gatttcctat tttttgcttt tttctgtagt
     5461 tttaaatctt ctttactgaa ggaaactcgt tcaccataca ctaaaaagcg gtgtgagttg
     5521 acaccttttg gtgtgcatgt cattaacgtg atcctatctt ggtctttttc aggcataaga
     5581 taatctaaat aatttttttg taattcttct gctttaactt cttcaattcg ataaatttga
     5641 taggcatgtt gttcttcatt taccttaata aagaattttt gccctttttt tactgaaggc
     5701 aaatcatcga aaagattatc tttataaagt ccactgtgcc ccgcgattaa aggattttta
     5761 ccgtttcctc ctgtaatatc gccagttcct tccgtaatcc ctacaccatt ctctaaaatt
     5821 ttgtcggaag ttccgtcata aattggtagt tttaaagaaa tacttggaat tgtaagaaca
     5881 ccaacaggat cgcctaactc tattttcgca acatccaaag aagaatctgt tttttcaaga
     5941 ttttcaatgg ctaaaattct ttgctgttgt tgaaatttcg ctacatttgt ttccacaact
     6001 ggaattaaat agactgctac tgccgacagc aaccaaaaaa atatcatcag tttgtgcatg
     6061 actcttcttt ttgggggctt acgaataatc attagctgat ccttctaggc tttcttctta
     6121 aagtaaacaa ctgcagaaag tccccctgct cctagcaaca agcaagagat caatacgtaa
     6181 gattggaaac cgttgctacc agtcattggt acggaagctt tttgattatt tttgatcgtg
     6241 attcggtttt cttctttgta tgaatctttg tctactttga aagagatctc ttttgttaat
     6301 ttttgatagt tttcaggagc ttttgtttct tccagtgagt aatctccttc agttaaacca
     6361 gtgatttcaa aatgaccctc tttatctgat tttaggattg ctgcatttgc tacatcgtct
     6421 acccaaactg cttctttgac accatcttta tctgtatcag taagtccacc gtagtactta
     6481 ccttctgcat tcttagctgc aaattctgcg ccttcaagcg gtgttgcttt gatacctgtt
     6541 ccaaataggc cgctatcgac ttttaagaat tttgcgccat aagtcacaac gtcagctttc
     6601 gttttttcgt ggtgttcttc ctgattaaat ggattattgt tataaacgaa atccacgcta
     6661 tttgttaatg gatttgcgat ttcagcacta tcattcacaa acatgttata agtcattttt
     6721 agatcttgac catgtaaatt cgcaactgta tcacttactt gtccattatt tacgataaaa
     6781 tcgatcacaa aaccactatc agaattttcg attttatagt cagtattttc ttttaatacg
     6841 actgaaccat cattatttgt tactttaata tctgcttttt cagcaacata agaaagtcct
     6901 ttacctgctg tatctcccca tttaaataca ctgtaaggag aagtttcgct tttcacaccg
     6961 tttactgtga taccgtctgc gccacctgca atgtctgttg gaatatgaat cgttccttga
     7021 taatttactg cgtcaccaat ttggaaagaa tgttcttgtc ctgttccatt tgttactgtt
     7081 ttatcgatca ctggtgcttt atagtttacg tatgaagctt ttaagtcact tgtcacgcca
     7141 tcattcgtaa tttcaaaggt taatttattg tgcgcatcgt tgcgagcgtc tgcgcctaat
     7201 aaatattgat ctgctgttgg ttcttgatct gatcccacta caacttccga aggaacttcc
     7261 acaaaatagt attttcctaa cgtcaaatca gtcgcagtca gctttccttg atcatccgat
     7321 acaagatcac ccaattttgt tccttttcca ggttcgccct tgtataactc aaattttgcc
     7381 ccttttaaaa gggtttgttt gctttggtct gcgccatcgt caccaaattt cgttaattca
     7441 aaactcaact tagaaacaat gttcttagga tacaaatgaa tattttttaa aaatcctgat
     7501 cctgttttat ctgtcattgg cgcaatcaca accattggtt ttgctttttg tgtgactaat
     7561 ccagcagcgg atttactttc ataaaccaca tatacattcc ctttggaatt gacataggct
     7621 ggttgatcag taaaagtgat ctcaccattt tcatctacag ctgaagtaat tttgttggtt
     7681 gcattcttga cgtactcact gttagatccc ttgtcttcta catcttgtac aatctcgtcc
     7741 actttcgcgt tggttagatc agaatcttct ctcggtaata cttgatccgt aatatttcct
     7801 aacgtaaact caacatcacc ataatctgcc ttgttgtatt tcgttacacc tgtaggatat
     7861 tgatcgtgcg tataaccatc gtttttgatc ccgtcaatat caacattcaa ttgcgttcct
     7921 tgatcgtaca ttaatttatg aaccgtgata ttgaccttct ctggtggtgt tttttgcgtg
     7981 acatcttctg catgtacttg atacccctga aagcagcctg caatgattcc taacattcca
     8041 acaagcattc cagctttttt tacataccga tttattgcca ttttttactc tcctcatttc
     8101 atatttcaac gactcgcgtt gattaaaacc ttgttattgt agctctccct gtaccacgta
     8161 tctttctttt ccttgtgacg tataaacaca agtaaacaac gttaataagg gtttctctcc
     8221 tttctttggc aaagacaaga aagagttctc ttcaggaacg ccatcgatta atttaaagta
     8281 cccaggtacg ataaatttac gcgttacctt gtaacgatag acgccatcgt tactaactaa
     8341 gtccatcacc tcccctaatg acaacttttg aatatcagaa aataaaacac ctggcatttc
     8401 catgttatga cctgctagga catagtttcc tttgcccatt tcggcatctt cataaaaata
     8461 agtagccgct ccaagactca aagtgtattg atttgcccct ttaaaaattg gcaatgctaa
     8521 accaagtgca ggaatctgaa tttgtgcaaa tacttgttta ttgacgccca tttcgattgc
     8581 ttctttttgt atggacaaca tttgccccaa agacatatca ttattgacgt tcgttgcatc
     8641 ctccttataa ctctttgcat gtgcattgat ttgatctacg ggcatctttt tttctatatt
     8701 ttgcttaaac acctctcctt catgtttttg gtagctaaca tagccaaaca cactgcaaaa
     8761 gacgattaaa gtgactccta aaaaaccaaa taaccatttt ttcatccatc tgcctccttt
     8821 cagacaaaac aaaaaaactt tttctcattt ccatagaaaa agttttaaaa atgaattcta
     8881 taagttcgtc tcaactgttc aaagtttttc attgaaccta acaaacaagc tttctctttt
     8941 cctgttaact gcatatcatt gatatattgc gcgacagata atcttttaag gtgtttttgc
     9001 ttcatcgttt ttagtaactt ttcttccatt tctttcgaca ctttttttct gttttcgatt
     9061 tcttcataat tcatgagttt tcctaccttc cttgtttttt gcaaaaataa aaaagcctat
     9121 gatcacatac gctctacact tcagtcttgg gttatctttt tctttcgttt aattactggc
     9181 cgtgctggtt tcaaatcctt gattccatta tgggttctcc aataatcgaa ttcttttcgc
     9241 aatctttctt tttccatatc tgttccttta gataaggctt tgaatacttc tttaggtgaa
     9301 taaccacgag ctaacagctg tacatacctt ccttttgtca aagtatccat atccatcgcc
     9361 tctaaaactg gtcgcgccat ctact
//
LOCUS       DAZKCE010000112         9285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000112 DAZKCE010000000
VERSION     DAZKCE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.113"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..684)
                     /locus_tag="ACG8KQ_002425"
     CDS             complement(16..684)
                     /locus_tag="ACG8KQ_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9105325.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            818..1105
                     /locus_tag="ACG8KQ_002426"
     CDS             818..1105
                     /locus_tag="ACG8KQ_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105326.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1521..3059
                     /locus_tag="ACG8KQ_002427"
     CDS             1521..3059
                     /locus_tag="ACG8KQ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9105327.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3056..4021
                     /locus_tag="ACG8KQ_002428"
     CDS             3056..4021
                     /locus_tag="ACG8KQ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105328.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4008..5330
                     /locus_tag="ACG8KQ_002429"
     CDS             4008..5330
                     /locus_tag="ACG8KQ_002429"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9105329.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5393..5497
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5571..6314
                     /locus_tag="ACG8KQ_002430"
     CDS             5571..6314
                     /locus_tag="ACG8KQ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9105330.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6329..7321
                     /locus_tag="ACG8KQ_002431"
     CDS             6329..7321
                     /locus_tag="ACG8KQ_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9105331.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7333..8070
                     /locus_tag="ACG8KQ_002432"
     CDS             7333..8070
                     /locus_tag="ACG8KQ_002432"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105332.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8060..8782
                     /locus_tag="ACG8KQ_002433"
     CDS             8060..8782
                     /locus_tag="ACG8KQ_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9105333.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8866..>9285)
                     /locus_tag="ACG8KQ_002434"
     CDS             complement(8866..>9285)
                     /locus_tag="ACG8KQ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9105334.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3039 a         1603 c         1964 g         2679 t
ORIGIN      
        1 agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc
       61 tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt
      121 attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc
      181 caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat
      241 cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc
      301 gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt
      361 gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa
      421 aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa
      481 tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa
      541 aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat
      601 attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc
      661 tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact
      721 tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg
      781 gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac
      841 aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca
      901 aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt
      961 tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg
     1021 tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga
     1081 gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa
     1141 aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga
     1201 acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta
     1261 ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat
     1321 agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg
     1381 cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct
     1441 gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga
     1501 ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg
     1561 agttgctgga agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag
     1621 cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct
     1681 tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta
     1741 tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc
     1801 ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag
     1861 cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg
     1921 cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta
     1981 ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc
     2041 gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat
     2101 tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag
     2161 cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg
     2221 aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata
     2281 aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga
     2341 cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag
     2401 gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa
     2461 aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat
     2521 taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg
     2581 aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat
     2641 tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct
     2701 tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg
     2761 gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag
     2821 tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa
     2881 aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa
     2941 taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga
     3001 cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac
     3061 taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga
     3121 cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg
     3181 aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta
     3241 cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata
     3301 tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat
     3361 ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca
     3421 aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg
     3481 agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat
     3541 ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt
     3601 gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc
     3661 aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac
     3721 accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc
     3781 gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga
     3841 aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc
     3901 gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct
     3961 gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg
     4021 atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag
     4081 caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg
     4141 gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat
     4201 tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca
     4261 aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa
     4321 tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag
     4381 aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag
     4441 tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta
     4501 aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg
     4561 ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg
     4621 cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg
     4681 gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga
     4741 cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt
     4801 tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc
     4861 tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa
     4921 aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa
     4981 caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg
     5041 ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc
     5101 ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct
     5161 ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga
     5221 tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata
     5281 tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat
     5341 aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt
     5401 tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc
     5461 agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc
     5521 ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa
     5581 atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag
     5641 tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg
     5701 cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt
     5761 tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt
     5821 tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag
     5881 ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct
     5941 tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag
     6001 agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata
     6061 aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg
     6121 tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga
     6181 tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc
     6241 tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc
     6301 tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact
     6361 gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga
     6421 tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta
     6481 cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc
     6541 aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag
     6601 ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat
     6661 tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca
     6721 ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc
     6781 tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg
     6841 aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga
     6901 aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa
     6961 agaattagat gaacggttag attactatac tccggtaatt attacgaatg atcaactgat
     7021 caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga
     7081 agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga
     7141 tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga
     7201 gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa
     7261 cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata
     7321 ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa
     7381 atattagaag acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca
     7441 agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct
     7501 ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg
     7561 ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa
     7621 aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg
     7681 ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg
     7741 gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga
     7801 gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa
     7861 gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac
     7921 aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa
     7981 aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag
     8041 ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag
     8101 tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga
     8161 agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat
     8221 ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc
     8281 aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca
     8341 aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact
     8401 gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta
     8461 tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta
     8521 tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt
     8581 cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg
     8641 ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc
     8701 tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg
     8761 tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt
     8821 ggctttcttt gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt
     8881 tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc
     8941 tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg
     9001 gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc
     9061 gatccattct tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg
     9121 agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac
     9181 aacaggttgt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc
     9241 ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAZKCE010000113         8950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000113 DAZKCE010000000
VERSION     DAZKCE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.114"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..778)
                     /locus_tag="ACG8KQ_002435"
     CDS             complement(554..778)
                     /locus_tag="ACG8KQ_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105335.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            969..3746
                     /locus_tag="ACG8KQ_002436"
     CDS             969..3746
                     /locus_tag="ACG8KQ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9105336.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3827..4246
                     /locus_tag="ACG8KQ_002437"
     CDS             3827..4246
                     /locus_tag="ACG8KQ_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9105337.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4258..4728
                     /locus_tag="ACG8KQ_002438"
     CDS             4258..4728
                     /locus_tag="ACG8KQ_002438"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9105338.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4745..5524
                     /locus_tag="ACG8KQ_002439"
     CDS             4745..5524
                     /locus_tag="ACG8KQ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9105339.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5511..6332
                     /locus_tag="ACG8KQ_002440"
     CDS             5511..6332
                     /locus_tag="ACG8KQ_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9105340.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6349..7398
                     /locus_tag="ACG8KQ_002441"
     CDS             6349..7398
                     /locus_tag="ACG8KQ_002441"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9105341.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7411..8415
                     /locus_tag="ACG8KQ_002442"
     CDS             7411..8415
                     /locus_tag="ACG8KQ_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9105342.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2994 a         1476 c         1843 g         2637 t
ORIGIN      
        1 taaatgaatt ctgcataaaa aatatatcaa gtaaatgttt atagatgaaa ctctatttac
       61 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
      121 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
      181 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
      241 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
      301 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
      361 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
      421 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
      481 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
      541 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
      601 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
      661 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
      721 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
      781 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
      841 actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga
      901 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
      961 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
     1021 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
     1081 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
     1141 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
     1201 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
     1261 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
     1321 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
     1381 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
     1441 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
     1501 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
     1561 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
     1621 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
     1681 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
     1741 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
     1801 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
     1861 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
     1921 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
     1981 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
     2041 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
     2101 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
     2161 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
     2221 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
     2281 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
     2341 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
     2401 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
     2461 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
     2521 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
     2581 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
     2641 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
     2701 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
     2761 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
     2821 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
     2881 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
     2941 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
     3001 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
     3061 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
     3121 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
     3181 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
     3241 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
     3301 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
     3361 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
     3421 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
     3481 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
     3541 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
     3601 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
     3661 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
     3721 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
     3781 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
     3841 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
     3901 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
     3961 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
     4021 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
     4081 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
     4141 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
     4201 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
     4261 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
     4321 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
     4381 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
     4441 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
     4501 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
     4561 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
     4621 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
     4681 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
     4741 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
     4801 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
     4861 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
     4921 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
     4981 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
     5041 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
     5101 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
     5161 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
     5221 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
     5281 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
     5341 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
     5401 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
     5461 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
     5521 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
     5581 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
     5641 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
     5701 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
     5761 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
     5821 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
     5881 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
     5941 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
     6001 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
     6061 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
     6121 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
     6181 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
     6241 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
     6301 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
     6361 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
     6421 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
     6481 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
     6541 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
     6601 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
     6661 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
     6721 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
     6781 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
     6841 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
     6901 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
     6961 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
     7021 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
     7081 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
     7141 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
     7201 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
     7261 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
     7321 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
     7381 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
     7441 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
     7501 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
     7561 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
     7621 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
     7681 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
     7741 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
     7801 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
     7861 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
     7921 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
     7981 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
     8041 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
     8101 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
     8161 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
     8221 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
     8281 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
     8341 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
     8401 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
     8461 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
     8521 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
     8581 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
     8641 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
     8701 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
     8761 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
     8821 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
     8881 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
     8941 acgataaaag
//
LOCUS       DAZKCE010000114         8853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000114 DAZKCE010000000
VERSION     DAZKCE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.115"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..2726
                     /gene="adhE"
                     /locus_tag="ACG8KQ_002443"
                     /gene_synonym="adhC"
     CDS             129..2726
                     /gene="adhE"
                     /locus_tag="ACG8KQ_002443"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9105343.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2955..4061
                     /locus_tag="ACG8KQ_002444"
     CDS             2955..4061
                     /locus_tag="ACG8KQ_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9105344.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4078..5106
                     /locus_tag="ACG8KQ_002445"
     CDS             4078..5106
                     /locus_tag="ACG8KQ_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9105345.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5112..5591
                     /locus_tag="ACG8KQ_002446"
     CDS             5112..5591
                     /locus_tag="ACG8KQ_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9105346.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5607..6902
                     /locus_tag="ACG8KQ_002447"
     CDS             5607..6902
                     /locus_tag="ACG8KQ_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9105347.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6913..7737
                     /locus_tag="ACG8KQ_002448"
     CDS             6913..7737
                     /locus_tag="ACG8KQ_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9105348.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7902..8801
                     /locus_tag="ACG8KQ_002449"
     CDS             7902..8801
                     /locus_tag="ACG8KQ_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105349.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2840 a         1536 c         1965 g         2512 t
ORIGIN      
        1 aagcaatttt atatttaaaa ataactgtga aaaaaatcac aaaaaagtgt ttacatagaa
       61 aaataaatat tgtatgatat acatgtgaat tatttaacaa ataaccatga cccattagga
      121 ggataatgat ggctaaagta atagaaaaag aaaaaacgaa gacaattgac gtacaagcaa
      181 tgattgatga tttggcagaa aaagcaaatg cagcattgaa agaaatggaa agctttgatc
      241 aagaaaaagt agaccatatc gtgcatgaaa tggcaatggc agctttggat caacatatgc
      301 cattagcgaa gatggctgta gaggaaacag gccgcggtgt ttacgaagat aaagcaatca
      361 aaaatatgta tgcgtccgaa tatatttggc ataatatcaa acatgataaa acagttggtg
      421 tgatcaatga agatgtccaa aaagggttga ttgaaattgc tgaaccagtc ggtgtggttt
      481 gcggagtaac tccaacaacg aatccgactt ccacaacgat tttcaaatca atgatcgcgt
      541 tgaaaacaag aaatccaatc gtttttgctt tccatccaag tgcccaaaaa tcttctgcag
      601 aagcagcacg agtggtacgt gatgctgcta tcgctgccgg agctcctgaa aattgtatcc
      661 aatggatcga acatccttcg atcgaagcga cttctatgct aatgaaccat ccaggtatcg
      721 cgatcgtctt agcgacaggt ggagctggaa tggtcaagtc tgcttactct acaggaaaac
      781 cagctttagg tgtaggtcca ggtaacgtac cagcttatat tgaaaaaaca gcaaaaatta
      841 aacgtgcagt gaacgatttg atcgtttcga aaacatttga taatggaatg atttgtgctt
      901 ctgaacaagc tgtgatcgtg gataaagaaa tctatgcagc agtaaaagca gaattccaag
      961 cacaccaagt ttatatcgta aaaccagatg aattgcaaaa attagaagat gcagtgatga
     1021 acgaaggaaa atatgcggtc aatccatcaa tcgttggaca ttctgcgatg gaaatcgcaa
     1081 aattggcagg aatcagtgta ccaaaaggaa cgaaaatgct gattgctgaa ttggaaggcg
     1141 taggcccaga ttatccgcta tcaagagaaa aactttctcc agttcttgcc atgatcaaag
     1201 ctaacaacac ggaccatgcg tttgatttgt gtgaaggtat gttgaatctt ggaggattag
     1261 gacatacagc agttatccat tcagaaaacg aagagttgca tgtcaaattc ggtcttcgaa
     1321 tgaaagcttg tcgtatttta gtgaatacac catctgcaga aggcggcatc ggtgatatct
     1381 ataacgaaat gattccgtca ttgactttgg gatgcggttc ttatggtaag aactctgttt
     1441 ctcgtaacgt ctctgctgta aacttaatca atgtcaaaac tgtagcgaaa cggagaaata
     1501 acatgcaatg gttcaaatta ccgccaaaaa tcttctttga aaagaactct ctattgtact
     1561 tagagaaaat ggaaaacgtt gaacgtgtca tgatcgtttg tgaccctggt atggtccaat
     1621 tcggttactg cgatacagtt cgtgaagtat tggcacgacg taaaaatgat gtgaagatcg
     1681 aagtcttctc agatgttgaa ccgaacccat cgacaaatac cgtatacgca ggaacgaaac
     1741 tgatggcaga cttcaagcca gatacagtaa tcgctcttgg aggcggttct gcaatggacg
     1801 cagctaaggg aatgtggatg ttctatgaac acccagacac atctttcttt ggtgcaaaac
     1861 aaaaattctt ggatatccgt aaacggacat ataagatcga caaaccagaa aaaacacaat
     1921 tcgtatgtat cccgacaaca tcagggactg gttctgaagt aacgccattt gcggtcatca
     1981 ctgacagtga aacacatgtg aaatatccgt tagcagatta tgcattgacg ccagatgtcg
     2041 caatcgtcga tcctcaattc gtcatgtcag ttccagcatc tgtcacagca gataccggga
     2101 tggatgtgtt aacacatgca atcgaatcct atgtttcagt catggcttca gattatacac
     2161 gtggattgag cctacaagca atcaagttag tctttgacta tttagaaaaa tcagtaaaaa
     2221 cacctgatat ggaatcacgt gaaaaaatgc acaacgcttc tacaatggct ggtatggcgt
     2281 ttgctaacgc attcctaggt atttgtcact cagtcgcaca taaaatcggc ggggaatacg
     2341 ggatccctca tggacggaca aatgcgattc ttttaccgca tattatccgc tataatgcaa
     2401 aagatccaca aaaacatgcg atgttcccta aatatgacta tttccgtgca gatacagatt
     2461 acgcagatat cgcaaaattc ttaggtctga aaggcgaaac aacagaggaa ttagtcgaag
     2521 ctttagcaac ggctgtttat gaattaggaa aatctgtcgg catcaacatg agtctaaaag
     2581 ctcaaggagt cacacaagaa acactagata caaccgtaga ccgtatggct gaattagctt
     2641 acgaagacca atgtacgaca gctaatccga aagaaccatt gatctctgaa ttgaaacaga
     2701 tcatcattga tgcttacaac ggataagcaa aagaaagcgt agaaaaaaga taagaaaata
     2761 gatgaaaaag aacttggaaa acgttgtttc tccaagttct ttttttaatt tccaagtaac
     2821 tgaaagttac ttttgttatt taacatttag gtcaatttta gacagttttt gttcaaaaag
     2881 cgatatttat agtggacggt attactgcta tattaaggat ataaaatgaa agcgtttaaa
     2941 aaggtggcgt aaagatgatt attaaagaag agatttttgt acctaaagaa ttagtgaaag
     3001 aaaaaattga cctgctcgtt gaaaacctga caacgatcaa agacgataat ggtgaatttt
     3061 tgttggactt tgatggactg aaagtagatg acaaaagctg gacagtgtgg aattggccac
     3121 aaggtgtcgg tctatatggt atttataaaa attaccagat gacaaaaaat cctcgcgctt
     3181 atcaagtggt caatgaatgg tttgaggatc gtatggaaga aggcgctccg cccaaaaacg
     3241 tcaatacaat ggcaccgcta ttgactatgg cttacttgta tgaagacacg aaagattcac
     3301 gctatttgcc ttatttagaa caatgggcag aatgggtcat gaatgacatg cctcggacaa
     3361 atgaagacgg gttacagcat gccacatatg gaccggaaaa taagaatcag ttgtgggacg
     3421 atacattgat gatgacagtg cttcctttgg caaaaatcgg gaaactgttg aatcgcccag
     3481 aatatttaga agaagctcgt taccagttta tgatccatat caaatacttg atggataaac
     3541 gaacaggatt gtggtatcac ggctggacgt ttgaaggaaa tcataactat gccgaagcat
     3601 tttgggctcg tggaaactgc tggttgacga ttgctattcc agagatcatt gaaatcttag
     3661 aattatcgaa agaagatgca ttgcgcaaat tattgatcga aacacttgaa gcacaagtgc
     3721 gtgcattaaa aacttatcag tctgaaagcg gtttatggca tacgttgttg gatgatcctt
     3781 cttcttacgt agaatcttct gcaactgcag gatttgctta cggtattttg aaagcggtcc
     3841 ataaacgtta tttaccacaa gaatataaag aagtcgctta tcgtgctatc caaggattgc
     3901 ttgaacaaat cgatgaaaaa ggagaagttc aaaacgtttc aatcgggaca ggaatggggg
     3961 acagtttaga tttttatcgg aatatcggaa tcacagcaat gccttatgga cagtcgttaa
     4021 cggtgctttg tttgacagaa ttgctcgtct cttattgtta aaagaaagga aacccatatg
     4081 aaaaaaacat cactgctgtt tgcagcaagt tcgcttagcc taggtctgct aggtctaagc
     4141 ggctgcggaa ataattctag tgtggcaact gtcgcagatg cagatgtaaa tgagatagtt
     4201 accttgcgct ttgcttatgc aagcaacagc cagccagtaa ttgatgcaat gaatgaattt
     4261 gggcgtttag taaaagaaaa aacagatggt tccgtacaga tccagtattt cccagatgga
     4321 caactgggtg gagaacgtga actgatcgaa ttgacacagt caggagcagt agatttcaca
     4381 aaagtaagtg cctcagcgtt ggaaagtttc tctaaagatt attcgatttt ttcagttcct
     4441 tatctattca atgatgaaga acatttcttt aaggtgatgg ataatgaaga aataatggag
     4501 ccaatctacc aatcgaccaa aaatttagga tttaccggat tgacctatta tgattctggt
     4561 cagcgcagct tttatatggt agacggtccg atccatacgc cggaagattt gaagggaaag
     4621 aagatccgtg tgatgcaaag cgaaaccgct atcaaaatgg tagagctttt aggcggttcg
     4681 cccgtaccta tgggaagtga tgaagtgtac acgtctcttc aatccaattt gatcaacgga
     4741 tcagaaaaca atgaatttgt cttgtataca gctggacatg gtggtgtggc taaatactat
     4801 tcctatgatg agcatacacg tgtgccagat atcatcatca tgaatgatgc catcaaagaa
     4861 cagctaactg atgagcagga aaaagccatt gaagaagcag cgaaagaatc aacggttttt
     4921 gaaattgaag cttttgcaaa agcaattgaa gaagaaaaag ctttagcaca agaagaacat
     4981 ggagtccaat tcaatgatgt agataataca ccgtttcgag aggcagttgc cccacttcat
     5041 gatatattca aaaacgatcc tacctacaaa gacctttatc aaaagatcca agagcaagga
     5101 gcgtagctgt gatgaaaatg attttaaaca aggtattaga gatcctggga gctgttttgc
     5161 ttgttgggat ggtcgctgtt gttttatggc aagtattttc cagaacggtt ttaggaaatc
     5221 caaatacggt cacggaagag cttgttcgat tcggtcttgt ctggtttgcc atgttgtctt
     5281 ccgcgtatgt tgtgggacaa aaagcacatt tagccgtaac ggttttaagt gaaaaattaa
     5341 ctggcaaaaa aagtaaaatt ttagaagttg ttgttcaaag tttatttctg gtatttgctg
     5401 cgatgatcat ggtgtatggc ggatggaatg ctgtcacatt gacaatgggg cagatctcgc
     5461 cttctttaag tattcctatg ggatatgttt atctgtcagt acctgtatcg ggggtactca
     5521 tcattatcta tagcctgatc aatctaatcg atacattaag caacaagcca gcaattcaag
     5581 aaaaaatata aaaatggagg aataaaatgg caatagaagc cggattagtg ttattttttg
     5641 tatttgttgt gttgcttgta ttagggatgc cgattgctat aagtattgca ttctcatcga
     5701 tgatgacttt attgttagtt ataccgtttg atgtttctgc ttttagttca gctcaaaaaa
     5761 tggtgggaag tatcaatagt ttttcgctga tcgctattcc gtttttcgtt ctctcgggca
     5821 ttatcatgaa taatggaggg atagctaaga aactagtgga ttttgcgatg ttgttcgtcg
     5881 ggcgcgtgcc gggggctttg gctcatacga atgtgttagg gaatgcttta tttggttcca
     5941 tctctagctc ggccattgcc gcttctaccg cgattggcgg agtgttgatc cctcagcaag
     6001 tggaagaagg atatgaccgg aagtttgcga cagcggtcaa tatcgcgtca gctccgacag
     6061 gtatggtcat tcctccaagt actgctttta tcatgtattc acttgttgct ggtggtgctt
     6121 cgatctctgc tttgtttatg ggaggttatc tcgtaggcgc tttgtgggcg ctaggaatca
     6181 tgctagtagc attcatcgtt gctaaaaaga ataaataccc tgttgtcgaa aaaggacaga
     6241 tggctcatgc tggaaaaata ataaaagagg cgattcctag ccttctattg attgtcatca
     6301 ttatcggtgg tatcttgacg gggatattta cagcagtaga agcttcagcg atagctgtca
     6361 tttactccct gttgattgct atgttttact ataaaacggt aaaactaaaa gatataccta
     6421 atatgttgtt tcaatcagta gcaatgtcag ggacgatcat gttcttattg gctacttctt
     6481 ccatgatgtc tttcgccatg gcgtttaccg gtattcctca ggcaattagt tcgttgatta
     6541 tgggcgtaac ggataataaa tttattattc tgttattagt caatcttgtg ttattactgg
     6601 tgggtatgtt tatggatgtg gcgcctgcta tcttgatttt tacaccaata tttttaccaa
     6661 tcgtcacaag tgtaggggtt gatcctgtac attacgggtt gttttcaatc atgaatctgt
     6721 gtgtaggctc catcacgccg ccagtaggta caggactata tgtcggtgca agtgtaggag
     6781 gcgtaaaagc agaggcaatg ctgaaaccgc ttcttccttt ttatggggtt atcttagtcg
     6841 tcttgttttt gattacttac ttcccgcaaa tcgtcatgtg gctacccaat atgctgagtt
     6901 aggagaatag ttatgttgaa cattacaatt agaggacatg atctttccga agtccaaacg
     6961 atagaagatc ttgcagaaaa aacaaaagaa caagggattc atacactgca gctcgcctta
     7021 ggtctttcgt ttcctgaaat accttctggc gcaaatgaga tcaattctgg gatgggaaat
     7081 tatgtcaaac gagttttaga aaagcaagaa gtgaccgtag gaattttaag ttgctatatc
     7141 aatatgattc atccagattt aacgatacga caagagttgc tgacaaagtt cgagcagtat
     7201 cttcgctatg cttcttcctt tggtgcgtcg atggttgctt cggaaactgg atgtgttttg
     7261 ccggaaatcc aatatacgga agaaaatttc acagatgaag catttgctga agctgtgtcc
     7321 gttatccgtc gcttggtaaa agctggagaa aaataccaaa tgatggtggg gatagaacca
     7381 ggtttgaatc atccggtgta ttctttagca agggtagagc agctgatcca agcagtagat
     7441 tcggactatc taggaatcat tttagatccg accaatttga tcacaagtag tgattatcaa
     7501 gaacaagtcc aattggtaga agaagctttt gaacgttttg gtgaaaagat atgtgcggtt
     7561 catttgaaag attttcgtgt cgaacaggaa aaaatcgtgc cggtaaattt gggagacggt
     7621 gtgatcgagt acacaaaaat caaagaaatc atcatgaaga atcgtccgta cctgtatgtg
     7681 gttcttgaag aaacaaaaga tgatgggatt cgttatggaa gaagtcttct tgaataatgt
     7741 aaagaagacg ttggttaact aatatttaga atggaggggg cggggctaga agatgccacg
     7801 tctcttcttt tttataagag tatataaaag cagctctttt tgattacaaa aaacgtggta
     7861 taataaaatt gtgcataagt gaacaaaagg aggtgcggca catgctccaa aagaaaatca
     7921 gcggtgaaca agtcaatacg atggtttctg atcagtatga aatctatcat gtcaaagatg
     7981 tcgtcagcca aaataaaacg atctatcatc atcatgattt ctatgagatc catgctacat
     8041 taaagggaga agcgactttt tatttgaacg gacgacaatt tacgatcaaa gcaggaagtg
     8101 tgctgctcat ccattccaat gatttgcatc gtatcgtccg ccaatcttca gatgtattcg
     8161 agcgtgtcta tatctttgtc accccttctt ttctagaaag tcgttccacg ccatggtcta
     8221 atctatcggc ttgttttcaa ccaatgggag agaagaggag tcgtgtattg caaatagaac
     8281 aggaagtatt gaagcaaaaa ttatcattta tcgaccatcc tcctgataaa aaaatttacg
     8341 gtgaagacat aaaatatgaa caagctctgg tagaatactt gatttttctc aatcaagttg
     8401 tacaaaaaga agagagtgcg caagaacaga atgctgtagt tcctaatgag cgtgtggaac
     8461 aaatgttaca atatatctca aaaaatttat ctcatccgct ttctttagag gaaatggaaa
     8521 agcagttttt tgtcagcaaa taccatatca cacgagaatt taaaaagtac actggattta
     8581 cgtttcatca gtatgtaatg aagaaaaagc tgatctatgc caagcacttg ttgcgagaat
     8641 accgcagctc tagtgctgta tatagtaaaa gtggtttttc ttcttatccg cattttctga
     8701 aagcattcaa gagagagttc ggcataacgc ctaaagaatt tttgaaacgt gatacacaga
     8761 aacagttggt tcattatgaa cattttgaag atgaacaatg agaatgatca ctaaaaaata
     8821 accgcagtag ctgctgttgt ttacatagaa gca
//
LOCUS       DAZKCE010000115         8836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000115 DAZKCE010000000
VERSION     DAZKCE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.116"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8KQ_002450"
     CDS             278..787
                     /locus_tag="ACG8KQ_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9105350.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8KQ_002451"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8KQ_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9105351.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8KQ_002452"
     CDS             2322..3302
                     /locus_tag="ACG8KQ_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9105352.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8KQ_002453"
     CDS             3424..4842
                     /locus_tag="ACG8KQ_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9105353.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8KQ_002454"
     CDS             4913..6382
                     /locus_tag="ACG8KQ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9105354.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8KQ_002455"
     CDS             6392..6859
                     /locus_tag="ACG8KQ_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9105355.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8KQ_002456"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8KQ_002456"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9105356.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..>8836
                     /locus_tag="ACG8KQ_002457"
     CDS             7849..>8836
                     /locus_tag="ACG8KQ_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9105357.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLD"
BASE COUNT         3040 a         1320 c         1779 g         2697 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagaca
//
LOCUS       DAZKCE010000116         8676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000116 DAZKCE010000000
VERSION     DAZKCE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.117"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..495)
                     /locus_tag="ACG8KQ_002458"
     CDS             complement(49..495)
                     /locus_tag="ACG8KQ_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9105358.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(522..698)
                     /gene="rpsU"
                     /locus_tag="ACG8KQ_002459"
     CDS             complement(522..698)
                     /gene="rpsU"
                     /locus_tag="ACG8KQ_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9105359.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(860..1288)
                     /locus_tag="ACG8KQ_002460"
     CDS             complement(860..1288)
                     /locus_tag="ACG8KQ_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9105360.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1380..2240
                     /locus_tag="ACG8KQ_002461"
     CDS             1380..2240
                     /locus_tag="ACG8KQ_002461"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9105361.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(2334..2684)
                     /locus_tag="ACG8KQ_002462"
     CDS             complement(2334..2684)
                     /locus_tag="ACG8KQ_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9105362.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2700..4427)
                     /locus_tag="ACG8KQ_002463"
     CDS             complement(2700..4427)
                     /locus_tag="ACG8KQ_002463"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9105363.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="ACG8KQ_002464"
     CDS             complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="ACG8KQ_002464"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9105364.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5711..7144)
                     /locus_tag="ACG8KQ_002465"
     CDS             complement(5711..7144)
                     /locus_tag="ACG8KQ_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9105365.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7144..8547)
                     /locus_tag="ACG8KQ_002466"
     CDS             complement(7144..8547)
                     /locus_tag="ACG8KQ_002466"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9105366.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            complement(8487..>8676)
                     /locus_tag="ACG8KQ_002467"
     CDS             complement(8487..>8676)
                     /locus_tag="ACG8KQ_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105367.1"
                     /translation="KIFTKIQKKTLTFVNVTGKKRISIVCFVYKKDNKSVKSKGVRTW
                     QKESKSLRLVEAAVTPPS"
BASE COUNT         2340 a         1946 c         1492 g         2898 t
ORIGIN      
        1 gaatttcttc accaacactc attgacgaag agcctttaaa tgataaaatt aagattgtaa
       61 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
      121 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
      181 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
      241 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
      301 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
      361 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
      421 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
      481 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
      541 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
      601 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
      661 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
      721 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
      781 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
      841 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
      901 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
      961 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
     1021 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
     1081 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
     1141 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
     1201 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
     1261 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
     1321 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
     1381 tgtcttccga ttctacagaa atcattactt tttttgtcat ctctgactct gctggagaaa
     1441 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
     1501 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
     1561 aagaaaaagc ccttgtccta cacacattga tcaatcaaga attgatcgag atgacgcgtt
     1621 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
     1681 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
     1741 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
     1801 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
     1861 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
     1921 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
     1981 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
     2041 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
     2101 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
     2161 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
     2221 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
     2281 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
     2341 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
     2401 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
     2461 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
     2521 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
     2581 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
     2641 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
     2701 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
     2761 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
     2821 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
     2881 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
     2941 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
     3001 gcagagattt ttgcagagcc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
     3061 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaatgtttt gccttgtttt
     3121 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
     3181 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
     3241 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
     3301 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
     3361 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
     3421 tgagcaaaca gggctccgat ttggtttcct gtcaatactt cataggagcc atttggcaaa
     3481 cgaacggctg cgcctaaacg gtctgcatca ggatccgttg caatcagtaa gtcagcgcct
     3541 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
     3601 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
     3661 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
     3721 tacacaagct tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
     3781 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
     3841 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
     3901 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
     3961 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
     4021 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
     4081 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
     4141 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
     4201 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
     4261 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
     4321 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
     4381 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
     4441 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
     4501 gaccaaaaaa cgcttaaatc aagaaaagat tcttttatca agcacggtta caagtattcg
     4561 cataaatcgg attcagtaga tttcacgcaa aaaaaagagt aaaagaacaa ttgaaaccgt
     4621 tctttcactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
     4681 cttgttgtcc agcaatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
     4741 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
     4801 gaatccagcc ggcttcgttc gtcaacccag cttctctaaa tggtgcagtt aatggatcca
     4861 atcctacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgcca gttttgacat
     4921 tacgtgcttt cacacctgtc actaccattt catttccaac gatttcgtca acgactgtat
     4981 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
     5041 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
     5101 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaatg
     5161 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
     5221 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
     5281 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
     5341 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
     5401 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
     5461 caggagcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
     5521 cagtcatccc agcaggccct gcgccaataa cgattacatc atacatatgt agattctcct
     5581 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
     5641 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
     5701 aaatcattga tcaatcgttc gaaagatttt ctccatttga atcaattact tttttatacc
     5761 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
     5821 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
     5881 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
     5941 cctggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa ccattctcat
     6001 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
     6061 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
     6121 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
     6181 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
     6241 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
     6301 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
     6361 aattttctcg atctgacttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
     6421 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcga
     6481 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
     6541 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
     6601 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
     6661 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
     6721 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
     6781 tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt ggctcttttt
     6841 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
     6901 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
     6961 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
     7021 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
     7081 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
     7141 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
     7201 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
     7261 ctgatgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
     7321 cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg ctgcttcgat
     7381 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
     7441 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
     7501 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
     7561 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
     7621 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
     7681 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
     7741 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
     7801 gttcatttgt tttaattgtt ctttgaaaaa cgggatgtca aagatatttt tcggcaatcc
     7861 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
     7921 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
     7981 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
     8041 gatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
     8101 cgcgaaatta atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
     8161 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
     8221 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
     8281 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
     8341 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
     8401 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
     8461 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
     8521 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
     8581 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac atttacaaaa
     8641 gttagtgttt tcttctggat ttttgtaaat atcttg
//
LOCUS       DAZKCE010000117         8569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000117 DAZKCE010000000
VERSION     DAZKCE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.118"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..2548)
                     /locus_tag="ACG8KQ_002468"
     CDS             complement(167..2548)
                     /locus_tag="ACG8KQ_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9105368.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2538..3185)
                     /gene="recU"
                     /locus_tag="ACG8KQ_002469"
     CDS             complement(2538..3185)
                     /gene="recU"
                     /locus_tag="ACG8KQ_002469"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9105369.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3249..3788
                     /locus_tag="ACG8KQ_002470"
     CDS             3249..3788
                     /locus_tag="ACG8KQ_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9105370.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3878..4297
                     /gene="gpsB"
                     /locus_tag="ACG8KQ_002471"
     CDS             3878..4297
                     /gene="gpsB"
                     /locus_tag="ACG8KQ_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9105371.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4319..4686
                     /gene="rnpB"
                     /locus_tag="ACG8KQ_002472"
     ncRNA           4319..4686
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8KQ_002472"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4895..6061
                     /locus_tag="ACG8KQ_002473"
     CDS             4895..6061
                     /locus_tag="ACG8KQ_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9105372.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6107..7603
                     /locus_tag="ACG8KQ_002474"
     CDS             6107..7603
                     /locus_tag="ACG8KQ_002474"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9105373.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7684..8367
                     /locus_tag="ACG8KQ_002475"
     CDS             7684..8367
                     /locus_tag="ACG8KQ_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9105374.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8376..>8569
                     /locus_tag="ACG8KQ_002476"
     CDS             8376..>8569
                     /locus_tag="ACG8KQ_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105375.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2681 a         1565 c         1823 g         2500 t
ORIGIN      
        1 aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc
       61 ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca
      121 tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag
      181 aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg
      241 gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag
      301 aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg
      361 aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg
      421 ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa
      481 ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta
      541 atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct
      601 cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggcgtc atttttttat
      661 tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg
      721 gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc
      781 cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga
      841 tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc
      901 catctggctc ataagtttct tctgttccgt cttggaagac gattttatta acatattgag
      961 gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag
     1021 aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat
     1081 ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga
     1141 cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag
     1201 taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt
     1261 tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc
     1321 cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt
     1381 tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc
     1441 cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat
     1501 actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat
     1561 aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga
     1621 cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca
     1681 gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt
     1741 tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat
     1801 aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag
     1861 aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc
     1921 cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt
     1981 gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg
     2041 cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca
     2101 gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga
     2161 tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta
     2221 attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat
     2281 cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg
     2341 catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg
     2401 agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc
     2461 cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc
     2521 tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat
     2581 aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct
     2641 attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg
     2701 tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac
     2761 cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga
     2821 tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag
     2881 tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag
     2941 gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg
     3001 gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg
     3061 attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct
     3121 tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat
     3181 accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg
     3241 aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg
     3301 gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac
     3361 tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg
     3421 aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg
     3481 gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa
     3541 aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat
     3601 cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc
     3661 tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt
     3721 accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta
     3781 gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag
     3841 aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc
     3901 taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt
     3961 tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag aactccttgc
     4021 tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg
     4081 agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt
     4141 tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca
     4201 acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac
     4261 aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt
     4321 tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg
     4381 tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg
     4441 gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac
     4501 gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa
     4561 acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc
     4621 agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt
     4681 atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac
     4741 aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac
     4801 aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc
     4861 ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct
     4921 cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg
     4981 aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc
     5041 tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga
     5101 tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct
     5161 tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac
     5221 acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca
     5281 ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt
     5341 gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg
     5401 ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat
     5461 gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct
     5521 ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc
     5581 ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga
     5641 agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca atggacggat
     5701 gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa
     5761 gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc
     5821 gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg
     5881 cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt
     5941 tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg
     6001 tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata
     6061 atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga
     6121 gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg
     6181 ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta
     6241 tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta
     6301 tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa
     6361 agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc
     6421 cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt
     6481 tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa
     6541 agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga
     6601 aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct
     6661 ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca
     6721 acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg
     6781 tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac
     6841 gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg
     6901 tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg
     6961 tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag
     7021 tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga
     7081 tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg
     7141 tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa
     7201 aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata
     7261 tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca
     7321 ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc
     7381 acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga
     7441 ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa
     7501 accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga
     7561 ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga
     7621 tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa
     7681 cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa
     7741 acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag
     7801 acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt
     7861 gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc
     7921 tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc
     7981 ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac
     8041 gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt
     8101 ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa
     8161 aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct
     8221 gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt
     8281 gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac
     8341 aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga
     8401 tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga
     8461 gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga
     8521 tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttatt
//
LOCUS       DAZKCE010000118         8281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000118 DAZKCE010000000
VERSION     DAZKCE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.119"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..2637
                     /gene="clpB"
                     /locus_tag="ACG8KQ_002477"
     CDS             28..2637
                     /gene="clpB"
                     /locus_tag="ACG8KQ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9105376.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2788..3474)
                     /locus_tag="ACG8KQ_002478"
     CDS             complement(2788..3474)
                     /locus_tag="ACG8KQ_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105377.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3452..3946)
                     /locus_tag="ACG8KQ_002479"
     CDS             complement(3452..3946)
                     /locus_tag="ACG8KQ_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9105378.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3966..5186)
                     /locus_tag="ACG8KQ_002480"
     CDS             complement(3966..5186)
                     /locus_tag="ACG8KQ_002480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9105379.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5333..7168
                     /gene="lepA"
                     /locus_tag="ACG8KQ_002481"
     CDS             5333..7168
                     /gene="lepA"
                     /locus_tag="ACG8KQ_002481"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9105380.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7364..8275
                     /locus_tag="ACG8KQ_002482"
     CDS             7364..8275
                     /locus_tag="ACG8KQ_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105381.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         2803 a         1495 c         1678 g         2305 t
ORIGIN      
        1 tacctttaat taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa
       61 gaagcgattg ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt
      121 gcccatgtat ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat
      181 gccggattgg atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca
      241 gttgtttctg gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta
      301 ttgaatgaag cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc
      361 gttattttag cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac
      421 ggattatctg aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg
      481 acctcacaaa accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta
      541 cagcaagtca aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat
      601 gtcatccgta ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt
      661 gtagggaaaa cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct
      721 gaaaatttaa aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca
      781 aaattccgtg gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt
      841 gatggacgta tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca
      901 gaaggaagta tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat
      961 ttgatcggtg ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg
     1021 gaacgtcgct ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt
     1081 ctgcgtggat taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca
     1141 ttagtagcag cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa
     1201 gcaatcgatt tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca
     1261 acggaattag atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg
     1321 aaaaaagaat cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct
     1381 gaacttcgtg aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc
     1441 aattctgttt cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca
     1501 gaaaataact atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta
     1561 gaaaaagaat tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa
     1621 gagtccgtta ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta
     1681 acgaaacttg tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa
     1741 cgagtgattg gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg
     1801 gctggattgc aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt
     1861 gtcggaaaaa ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat
     1921 atggtacgga tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga
     1981 gctcctccag gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga
     2041 aatccttata caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat
     2101 atcttgttac aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat
     2161 ttcaaaaata cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc
     2221 gtgactgctg atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt
     2281 ggaaacttca aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg
     2341 agcctagaca acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg
     2401 gaacatcaag aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat
     2461 gcttatgaac cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa
     2521 acaccactag ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt
     2581 actttacttg atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct
     2641 tccattgaga ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt
     2701 tcaaataaac agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc
     2761 tgcccgcttc tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact
     2821 ttcaaagtaa cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc
     2881 acctatcgta tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa
     2941 cagtgtgcca tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa
     3001 gaattcccag aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag
     3061 gaacaaccaa ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc
     3121 atagccaaga gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga
     3181 gatgatactg atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca
     3241 gtaaaacaaa acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg
     3301 catgaaaatc aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt
     3361 cgaatatttt tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc
     3421 aagtaaagct atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa
     3481 atctttttac ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga
     3541 tcaaacctat tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg
     3601 cagcgatttt tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca
     3661 ctgggatgcc tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca
     3721 gcccatctag taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag
     3781 caatcattcg actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa
     3841 gatagatcga aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata
     3901 acaaatcact tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct
     3961 ttttatcaaa aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac
     4021 ttcagtgttt cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga
     4081 taattcatca tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt
     4141 tcatttaaga cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc
     4201 caaggagcga caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga
     4261 aaccgttgaa ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc
     4321 tctttgatct gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca
     4381 tgtacccagt agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct
     4441 tcttgatcaa gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac
     4501 agaacacctc ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca
     4561 ttttcgaaac gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt
     4621 tgttgggcaa atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca
     4681 accataccac ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt
     4741 ttatcagaca tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa
     4801 taagagatcc attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc
     4861 atcaatccat ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta
     4921 taacgtgcac cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta
     4981 ttttcgtgga accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata
     5041 aaagctcgtt tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca
     5101 taggaaagca attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga
     5161 ttttctctaa aatctttttg atccattttt tcatctccat ttattagctt gataacagta
     5221 tagcaaaatt ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact
     5281 gcttttcgtt ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat
     5341 aaagaaaatg aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga
     5401 ccatgggaaa tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg
     5461 cgaaatgcaa gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat
     5521 taaattaaat gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca
     5581 cttgattgac accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc
     5641 ctgtgagggt gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc
     5701 caatgtttat ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga
     5761 cctacccgca gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga
     5821 tgctagtgaa gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga
     5881 gcagatcgtg gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt
     5941 gatctttgac tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga
     6001 cggtatggtc aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt
     6061 tacagaggtc ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga
     6121 tgtcggttat atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt
     6181 aacgttagca gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat
     6241 ggtttattgt ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt
     6301 agaaaaactg caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt
     6361 aggttttggg ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg
     6421 cttggaacgg gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt
     6481 taataaaacg gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt
     6541 aacgattcag gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt
     6601 tgtcggtgca gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta
     6661 tttagatgat taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga
     6721 ctttttcgac aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc
     6781 tggttatcaa aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga
     6841 tgcattaagc tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga
     6901 aaaactgaaa aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg
     6961 acaaaaaatc gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg
     7021 ttatggtgga gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa
     7081 gcggatgaaa caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa
     7141 aatggatgag caagatagtg tgaaataact tataatcaat cgaaaaacct attaaataaa
     7201 cctttttgag tagatgcaac actgaggatt atctgtataa aagattttaa actgtcattt
     7261 ggttatcacg gcgatactaa aattaacaaa attggaatat ttttcttgaa gatttttagc
     7321 tgtcagtata tgatgaaagc atctcatagg aggtgctttt ttcatggttg ataattcaag
     7381 tggaagggtt cttaaatctc taagaaaaga gaaaaaacta tctcaaaaaa agttagctga
     7441 tttagcaggt atttcacaaa gtactttagt taaatatgaa aaagggtcaa gaaaaatccc
     7501 taaagacgta gataatactc taagcaagat tctgaatata gaaactctaa taaaagatga
     7561 ggaagataag attgagattc tcatcggtaa actaattgca tacagagata tgaataagtt
     7621 acttaacaaa gagttagctg ataatatagg aattagtgaa gtattactca gttatgtgtt
     7681 aaatagaaag agaaatccgt caaaagaaat gcaaaaaaaa atcgatattt ttctattaag
     7741 taatgaaaaa gaaattttga aggagattaa tcgtgatagt gagatattta gtttatcaaa
     7801 agacgataaa attgttatgg gaaaaagaat acgagaagtc cgaaagaacc gggaagagac
     7861 gcttgaaaaa tttggtaaaa actttaccat ctatacaggc aaaaatgtta taagtagatg
     7921 ggaaaaaggt attaatattc cagatattga aaaactaatg aatattgcct atcttggtaa
     7981 ggtcactgtt ccttacctta tgtacggaga agattataag aatatacttc caaaagatga
     8041 aagagttagc gattttaaaa agataaattc ttttagtatg ggattaagga tgaggaaaat
     8101 taggaaagat tattatctag aaagggagga gtttggaaag cttttttctc cttcaatttc
     8161 aaaatggtcc atagataggt atgagaatgg tagagatata cccaatacta atcgaattat
     8221 ccaatatgct tatataggaa atctttcttt agaatttttg atttacggga tataaaaata
     8281 g
//
LOCUS       DAZKCE010000119         8272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000119 DAZKCE010000000
VERSION     DAZKCE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.120"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            348..617
                     /gene="rpsN"
                     /locus_tag="ACG8KQ_002483"
     CDS             348..617
                     /gene="rpsN"
                     /locus_tag="ACG8KQ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9105382.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            632..811
                     /gene="rpmF"
                     /locus_tag="ACG8KQ_002484"
     CDS             632..811
                     /gene="rpmF"
                     /locus_tag="ACG8KQ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9105383.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            843..1220
                     /locus_tag="ACG8KQ_002485"
     CDS             843..1220
                     /locus_tag="ACG8KQ_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105384.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1241..1390
                     /gene="rpmG"
                     /locus_tag="ACG8KQ_002486"
     CDS             1241..1390
                     /gene="rpmG"
                     /locus_tag="ACG8KQ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9105385.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1414..2397
                     /locus_tag="ACG8KQ_002487"
     CDS             1414..2397
                     /locus_tag="ACG8KQ_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9105386.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2427..2549
                     /locus_tag="ACG8KQ_002488"
     CDS             2427..2549
                     /locus_tag="ACG8KQ_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9105387.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2567..4090
                     /locus_tag="ACG8KQ_002489"
     CDS             2567..4090
                     /locus_tag="ACG8KQ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9105388.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4114..4353
                     /locus_tag="ACG8KQ_002490"
     CDS             4114..4353
                     /locus_tag="ACG8KQ_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105389.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4546..5007)
                     /locus_tag="ACG8KQ_002491"
     CDS             complement(4546..5007)
                     /locus_tag="ACG8KQ_002491"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9105390.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5202..5420)
                     /locus_tag="ACG8KQ_002492"
     CDS             complement(5202..5420)
                     /locus_tag="ACG8KQ_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105391.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5538..7154
                     /locus_tag="ACG8KQ_002493"
     CDS             5538..7154
                     /locus_tag="ACG8KQ_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9105392.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7395..>8272)
                     /locus_tag="ACG8KQ_002494"
     CDS             complement(7395..>8272)
                     /locus_tag="ACG8KQ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9105393.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2831 a         1276 c         1655 g         2510 t
ORIGIN      
        1 attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat
       61 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
      121 aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga tttgattatc
      181 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      241 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      301 ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      361 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca gataaacgtc
      421 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      481 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      541 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      601 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      661 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      721 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      781 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      841 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      901 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
      961 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
     1021 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1081 acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat atgaaagaaa
     1141 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1201 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1261 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1321 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa tttttaaaga
     1381 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1441 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1501 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1561 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1621 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1681 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1741 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1801 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1861 gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1921 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     1981 agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca tcccttagtt
     2041 tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca acaccatcaa
     2101 gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2161 ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2221 ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa cgaacaggtt
     2281 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat cggaaaaatg
     2341 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2401 taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2461 gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2521 aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2581 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2641 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2701 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2761 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     2821 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     2881 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     2941 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     3001 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3061 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3121 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3181 tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga aacaagttcc
     3241 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3301 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3361 tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc ctcttgaaag
     3421 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3481 aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3541 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3601 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3661 ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg ccgagtacaa
     3721 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3781 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     3841 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     3901 aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac caagtaaggc
     3961 agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata atgaattgca
     4021 taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc aagaaatgat
     4081 ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4141 gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat gagattaaat
     4201 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4261 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4321 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4381 cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca
     4441 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4501 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4561 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4621 atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg cgaaatgatt
     4681 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4741 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4801 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac caccagtttt
     4861 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     4921 acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat gattttttgt
     4981 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     5041 gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa aactaattga
     5101 aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc actgaaaatc
     5161 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5221 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat agtttttttc
     5281 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5341 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc gagtatagta
     5401 agatgacgat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5461 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5521 atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc tatcttggtt
     5581 caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag gaaattgctg
     5641 tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac aagaaaggtt
     5701 ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt tgtacattac
     5761 gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg aatcaaattg
     5821 ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt atattagcta
     5881 aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt attatagtag
     5941 tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag gaaagaaaag
     6001 ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt gatcaattcg
     6061 agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag acgatttaca
     6121 aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg acaattaaag
     6181 gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat gagacttcct
     6241 attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt cctgaaacag
     6301 aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac gcacaaaaat
     6361 ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa gggtttcttt
     6421 ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc tataaaagta
     6481 ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa atgattatac
     6541 aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac
     6601 tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt
     6661 taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaagt
     6721 ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag
     6781 gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta tttgtattct
     6841 ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcgaattg
     6901 atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc
     6961 atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag
     7021 aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa
     7081 cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa agcacagatt
     7141 actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta
     7201 ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg
     7261 gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta
     7321 gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg
     7381 agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc
     7441 agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa gattcgttct
     7501 cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt gatatggtta
     7561 ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt gatttctgtt
     7621 tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct
     7681 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc
     7741 tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg atcgactatc
     7801 tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg acctttcaat
     7861 tcccacgcat tcttttgaag tagtttccag tatttcttta agtgcttcga ccgacgatcc
     7921 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg accaatgtgt
     7981 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
     8041 gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg gacttttata
     8101 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
     8161 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac atttttcact
     8221 gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga aa
//
LOCUS       DAZKCE010000120         8048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000120 DAZKCE010000000
VERSION     DAZKCE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.122"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACG8KQ_002495"
     CDS             complement(17..538)
                     /locus_tag="ACG8KQ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9105394.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACG8KQ_002496"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACG8KQ_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9105395.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG8KQ_002497"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG8KQ_002497"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9105396.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACG8KQ_002498"
     CDS             5502..6671
                     /locus_tag="ACG8KQ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9105397.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..>8048
                     /gene="glmM"
                     /locus_tag="ACG8KQ_002499"
     CDS             6695..>8048
                     /gene="glmM"
                     /locus_tag="ACG8KQ_002499"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9105398.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2580 a         1489 c         1827 g         2152 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gattaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggatt
//
LOCUS       DAZKCE010000121         7967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000121 DAZKCE010000000
VERSION     DAZKCE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.123"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..749
                     /locus_tag="ACG8KQ_002500"
     CDS             48..749
                     /locus_tag="ACG8KQ_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9105399.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1358..2374)
                     /gene="tsaD"
                     /locus_tag="ACG8KQ_002501"
     CDS             complement(1358..2374)
                     /gene="tsaD"
                     /locus_tag="ACG8KQ_002501"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9105400.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2371..2835)
                     /gene="rimI"
                     /locus_tag="ACG8KQ_002502"
     CDS             complement(2371..2835)
                     /gene="rimI"
                     /locus_tag="ACG8KQ_002502"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9105401.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2842..3384)
                     /gene="rimI"
                     /locus_tag="ACG8KQ_002503"
     CDS             complement(2842..3384)
                     /gene="rimI"
                     /locus_tag="ACG8KQ_002503"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9105402.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3368..4090)
                     /gene="tsaB"
                     /locus_tag="ACG8KQ_002504"
     CDS             complement(3368..4090)
                     /gene="tsaB"
                     /locus_tag="ACG8KQ_002504"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9105403.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4281..5261)
                     /locus_tag="ACG8KQ_002505"
     CDS             complement(4281..5261)
                     /locus_tag="ACG8KQ_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9105404.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5285..6769)
                     /gene="galT"
                     /locus_tag="ACG8KQ_002506"
     CDS             complement(5285..6769)
                     /gene="galT"
                     /locus_tag="ACG8KQ_002506"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9105405.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6781..7770)
                     /gene="galE"
                     /locus_tag="ACG8KQ_002507"
     CDS             complement(6781..7770)
                     /gene="galE"
                     /locus_tag="ACG8KQ_002507"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9105406.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2248 a         1707 c         1407 g         2605 t
ORIGIN      
        1 aaaaaagtga caataaatat agtgaacaat aaggagagga ctttttaatg cgtgttaata
       61 aaatcgattt tcacttttca aaactcagat atcaacctga ttttgaacat ttctcggatg
      121 aagagttcca acagatcaag gaacatactg ttcttcgttc ttataaaaaa ggacagatcc
      181 ttttcgacga tggtgatccc agacaacggt tgtatatctt gaccaaagga ctcgttcgtt
      241 tagaacgtta tgatgaaagt gcaacttatt actactatga ctatgtgaat acaaataacc
      301 tttttcctat gtcaggaatg tttggagaag agacctacac ttatacagct caagcgatga
      361 ctgatattga aacattctac cttcctgttt cctactatga aaaattagca cgaaaaaatc
      421 ccgaacagtt gatctatatg atacaaaaaa tttctaaagt agttgaaata catgaattgc
      481 gtatccaaat cggattgacc tctagcgcct ttgaccgggt aaaacaaaat ttattcattt
      541 tgaaagaaga attgggcgta ccaacaaaag atggtcatat caccattcct tatccgatca
      601 ccttaaaaga attagcagtg aatagcggca cgacacgtga aactgctgga caagtaatca
      661 aacaactacg tgattccggg ctgttagatt atgacaaaaa gcgttttact ttctgtaaga
      721 cagctactac tccagctatg agagaataac gcgaacaaaa ataagcagtg accctgataa
      781 atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg cagttttaga
      841 tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata ttcactgttt
      901 gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga tctagattat
      961 tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa cggataatga
     1021 taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt aaaaaatagg
     1081 gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc tgtcaactct
     1141 tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt
     1201 gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca
     1261 tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc
     1321 ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa accaagcttg
     1381 gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg
     1441 ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct
     1501 cagggagttt agttgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta
     1561 cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga agtgttttag
     1621 taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat
     1681 cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa
     1741 aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa ttatcttttc
     1801 cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc
     1861 caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg
     1921 atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca
     1981 atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta
     2041 caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca
     2101 gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg
     2161 acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc
     2221 ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt tgagaagcaa
     2281 cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac
     2341 ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct
     2401 gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat
     2461 acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa
     2521 aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc
     2581 atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat
     2641 tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa gcagataatc
     2701 agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg
     2761 ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt
     2821 ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc
     2881 ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc ccaatttccg
     2941 ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg
     3001 attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg
     3061 atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc
     3121 gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga
     3181 cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg
     3241 ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg
     3301 atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc
     3361 atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt
     3421 tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg
     3481 gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa
     3541 ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat ttgtgacaat
     3601 cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag
     3661 cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag cggtatgccc
     3721 cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta tccacaccga
     3781 tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat
     3841 aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat
     3901 cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt
     3961 cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa
     4021 gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta
     4081 atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact
     4141 tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac atgattatct
     4201 gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat cactccattt
     4261 tcccacagct tttccatctt ttatttcgtt gattctcgaa taatcaactc tgtttccaca
     4321 ataatctttc ttgaaaccgg tgctttggaa ttacttaatt gaagaatcgt ctccactgct
     4381 agctctccca tccattcggt ataaactttt acagtagaaa gcgagggcgt tacatatttc
     4441 gctacactga catcattgaa gcccatcaca gaaatatctt ctggtaccac taaacctttt
     4501 tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat tagctgcaaa aagcgcatca
     4561 ggaacatgcg gattcgtttt taaatactgc ttcactgctt gatatcctga ctcgacagaa
     4621 aaatctgctt cgataattgg accttcgtcg tagccttttt ctgccaaaac ttgttgaaat
     4681 gccataattc tgggatcttt tagccgctga tggtttttct tcgtatattc tgttccagca
     4741 agcatagcga tatgttgcgc tttatgggag acaaagtgtt caatgacttt ttgaacactt
     4801 gacttaaagt cgacaactag agagtcgatt ccttgagaag tgccgtcaga atctaccaaa
     4861 agcaaggcat tttgggatcc tttcatttct aataactgag cttcgtcgaa ttttcctagt
     4921 gcgatcgttc catctgccgg aagatcacta atggaatccc acgtttcttt caataaagta
     4981 acgtttgctt cttctgcctt tttttcgatt cctaggcgaa tagataaata ataaagatct
     5041 tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa gcgtttgctt agtatttgct
     5101 gcatttttac gatgtttcgt ataatttaag cgttctgctg cttcaaaaat tttttgcttt
     5161 gtggctaagc caacggaaag atcaggatca tagttgagaa ctcttgaaac agtagcagga
     5221 gaaactcctg ccaactgcgc aatgtcttta attgttgcca tttgtctctc ctcctttttt
     5281 acgtttataa aatcgcagta aatcgttcaa acgctgcctg tcctttttca tctcgcttga
     5341 atactccagc gtcttccagt acacgtgcaa aaacctgacc tacagatttt tgtacgacat
     5401 cggtgaccgt atctttcgtc aaatcaggat gttctttttt taattgttct gcccacggac
     5461 gatgatattc agccatctgg ttttcttctc ctaataagaa atgctctact tctactagtt
     5521 ctggtttcaa acgaggtggt aaaacagcaa gccccattac ttcgatcaac ccaatgttct
     5581 cctttttgat atgttggaca tctttatgtg gatgaaaaat cccatcagga tgttcttctg
     5641 aaacattgtt gtctcgaagg acaaggtcca actcaaagta attgtccctt cttcgtgcaa
     5701 ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa agctctgata gatactgttt
     5761 catccgaata tgctttccat ttttccaata ctgagttagc tgcttcaata agcaagtttt
     5821 tctctgttga ttgcaaacgg atcacagaca tcggccattt cacgactcct gcgatgactt
     5881 gcggataatc ttctagtttg ataagttttt ctatcggtgc ttttgccata ggaaattcat
     5941 gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc tccaacaatt ggtaagtcag
     6001 cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat ccgaagcagt ctagcaaatg
     6061 tttcctttgt gattttcatc gggcggtgtt gttctgataa aatgatcgaa tgttcattgt
     6121 aataagcata aggggaatat tggaatcccc agctttcacc gtctagattc attcgaataa
     6181 tccggtgatt ggtgcgggct ggatagttca agcgacctct atatccttcg ttttccatgc
     6241 aaagcatgca ggtaggatag tctactttta ccgcctcgcg ttctgctgcg atttgttttg
     6301 gatctttttc tggcttagat aaattgattg tgatttccag ttcaccatac tccgactctg
     6361 ccggaaattt gatgtttttt gcgatggctc ttgttttgat gtagtcattt ttcttactta
     6421 aattaaagaa atactcagtt gcttcctctg ggcttttcga atagtgctgt gcaaagaatg
     6481 cattgacgac tgaaggtggc ggtgtgagaa aatccattaa ttgcgcttca aaaatctctt
     6541 gttctgctgg caattctgaa agaacaccat tttctcttgc cttagcgaca agctgatcca
     6601 ataagtctac agattcttcc ttaactggtc ttacttcaat gctttctaga gcatcttctc
     6661 ctaccatccc taaaagacga ttctgtaaat ataagcggtc catttccatc catccgccag
     6721 cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact gattgacata tctcattccc
     6781 ctattcttca taaccatttg gatggctgac atgccaatcc catgctgttt tgataatcgc
     6841 tttgatatct gtgtattcag gtttccagcc cagaatctct ctcgcttttt cacttgaagc
     6901 gactaatcgg ctaggatctc cagctcttct tggtgctatt tctgctggaa tttcttttcc
     6961 tgtcacttct ctagctgctt ctagcatttc cttgactgag tatcctttgc tgctgcctaa
     7021 attgaagaag ttgctttcat ttccttcttt caagtattcc aaagctaaaa tatgtgcagc
     7081 aatcaaatct tctacttgaa cataatctct gatacatgtt ccatctggtg tatcataatc
     7141 gtctccataa acagccaagg cttttctttg accaagagct acttgtaaaa tgattgggac
     7201 aagatgcgtt tctggtgtat gatcttctcc gatagatgca tctgcttttg ctccggctac
     7261 attgaagtag cgcaaagcga catagcgcat gccatatgct tggtcacacc acttcatcat
     7321 tttttccatc atcaatttac tttcaccata cggattttta ggattagctg gtgtatcttc
     7381 tgtgattgga gactctttcg gctctccata agttgctgca gtagaagaga atacgatttt
     7441 attcacgttg aactcgtgca tgacttcaag taagatttgc ataccgtata cgttgttgtt
     7501 gaaatacatc aatggttttt cgacagattc tcctactaaa gagcttgcag caaaatggat
     7561 tactccttca atcggttcct tttcaaatac ggaacgtaga aattctttat cccggatgtc
     7621 tccttcataa aaatgagcat ctggatgtac tgcttgtttg tgacctgtga gcaaattatc
     7681 taccacagct acttgatacc ccttttgaac tagctggtct acagcgtgcg aaccgatata
     7741 accggctccg cctagtacta aaattgtcat aaaaaacact cctttgatta gtaaaattgt
     7801 gtttgttgat tctatataat ttaaaaaacg aagtttactt ttatggtgaa aacaaacaaa
     7861 gtaaaatttc gtaaattcat atggtaaatt tataataaaa catttagtaa acattttcaa
     7921 ggattttttg caaaggttta caaacaaacg taaaactagc ctttttt
//
LOCUS       DAZKCE010000122         7769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000122 DAZKCE010000000
VERSION     DAZKCE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.124"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1381)
                     /locus_tag="ACG8KQ_002508"
     CDS             complement(17..1381)
                     /locus_tag="ACG8KQ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9105407.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1378..2937)
                     /locus_tag="ACG8KQ_002509"
     CDS             complement(1378..2937)
                     /locus_tag="ACG8KQ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105408.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2993..5473)
                     /locus_tag="ACG8KQ_002510"
     CDS             complement(2993..5473)
                     /locus_tag="ACG8KQ_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105409.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5457..5792)
                     /locus_tag="ACG8KQ_002511"
     CDS             complement(5457..5792)
                     /locus_tag="ACG8KQ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105410.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5806..6840)
                     /locus_tag="ACG8KQ_002512"
     CDS             complement(5806..6840)
                     /locus_tag="ACG8KQ_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9105411.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6930..7610)
                     /locus_tag="ACG8KQ_002513"
     CDS             complement(6930..7610)
                     /locus_tag="ACG8KQ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9105412.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2223 a         1554 c         1419 g         2573 t
ORIGIN      
        1 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
       61 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtattca gacagaaatc
      121 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
      181 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
      241 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg tattgtggaa
      301 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
      361 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
      421 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
      481 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
      541 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
      601 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
      661 gggcagaaaa aatgaccata tctgccttta ttgtttgaga cagaagttgt acggatacta
      721 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa tttatttgct
      781 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa
      841 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
      901 attccaatcc aatgtaacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
      961 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
     1021 gtttatacaa ggtgacagga gaggaacctg tcgctacgat taatttatca taggttatgg
     1081 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
     1141 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
     1201 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
     1261 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
     1321 ttcttagatt tagggcagct gtgatgcctt catgggaagc accaatgatc acgattttca
     1381 tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa
     1441 ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag cggacttctg
     1501 tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta
     1561 ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat
     1621 atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg
     1681 cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc
     1741 ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa
     1801 aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa
     1861 aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc
     1921 aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg
     1981 tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa
     2041 ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa
     2101 aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa
     2161 tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata
     2221 atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc
     2281 aaatttcaat cgtccttctt ctgtaatagc aaaactaaac tctttttgca gcagatctaa
     2341 aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagatagg gacgtccaaa
     2401 ccaaaataat tggaaataaa aataactaat ttgactttct ggaccggtca gccgcccgct
     2461 ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa
     2521 agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc
     2581 caacagataa agaagtatct gatattttgt agaatagcgc aagtaacaat gataataaac
     2641 atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat
     2701 ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt
     2761 agcccgatca actgataagc gggtaagtac gttattaacg gaaacctctc ctttttcctc
     2821 taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag
     2881 ttccaagtcc actccttcaa gcatttctga ttttaacagc agaagtttcc ttctcatcct
     2941 tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg
     3001 ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat
     3061 tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct
     3121 ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt
     3181 gctttgatcc agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat
     3241 tcttaaataa agaagtcaat gtacctgtca gttgccaagg agacgtgctt ggaagccgag
     3301 tatcccgcac atcaaaagaa aaatcgcctt ttgattcata ataacttgtc ttcaacggtt
     3361 tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt
     3421 tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat
     3481 ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta
     3541 aagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt
     3601 tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg
     3661 taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga
     3721 tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa
     3781 tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt
     3841 ttagctgact atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg
     3901 tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta
     3961 catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag
     4021 aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc
     4081 atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt
     4141 ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc
     4201 cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct
     4261 ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca
     4321 cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc
     4381 cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca
     4441 actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg
     4501 gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc
     4561 ctgttgaatc acttggtgag atgtaactat tccacgtatt tatctctgca cttgcagaaa
     4621 atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag
     4681 ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat
     4741 caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt
     4801 tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa
     4861 catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa
     4921 tatctgtcaa tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc
     4981 ctgctgataa taaacttccg ctagtaacct tgatgggggc accggcaata tacaagtagg
     5041 cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga
     5101 tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta
     5161 gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac
     5221 catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa
     5281 gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt
     5341 atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga
     5401 taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat
     5461 tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga
     5521 agccataatt ctataccaat gatcaaataa agaaaacgga tcttttgttc attcgtctcc
     5581 gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat
     5641 gcgccattga ctgaaccact tggatgcgga attggactta gatcataaaa tgtaatcgta
     5701 cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca
     5761 ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc
     5821 ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc
     5881 agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt
     5941 ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc
     6001 attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc
     6061 accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa atgcattaga
     6121 cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct
     6181 gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc
     6241 gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa
     6301 aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg tagcattttc
     6361 tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct
     6421 ttttgctggg attttcactt ctataggtac agttgtttct gcatgtgctg atacattgat
     6481 ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt
     6541 tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga
     6601 gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt
     6661 ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg
     6721 aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa
     6781 aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat
     6841 tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc
     6901 accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa
     6961 gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct
     7021 ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt
     7081 gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg
     7141 ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact
     7201 tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca
     7261 tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg
     7321 tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcggga
     7381 actacatcca gtgttaaagg acctgtctgg ccagttgctg gattttccgg atctgctggc
     7441 tctactggtt ttgaaggatc agtagggtta agaggctcca cggtatttgt tggttcagta
     7501 aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg
     7561 cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac
     7621 tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc
     7681 atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt
     7741 ttacatgaat attcttcctt ttttattaa
//
LOCUS       DAZKCE010000123         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000123 DAZKCE010000000
VERSION     DAZKCE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.125"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG8KQ_002514"
     CDS             complement(136..426)
                     /locus_tag="ACG8KQ_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105413.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG8KQ_002515"
     CDS             complement(544..804)
                     /locus_tag="ACG8KQ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105414.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG8KQ_002516"
     CDS             complement(941..1354)
                     /locus_tag="ACG8KQ_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP9105415.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG8KQ_002517"
     CDS             complement(1810..2295)
                     /locus_tag="ACG8KQ_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105416.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACG8KQ_002518"
     CDS             complement(2434..2649)
                     /locus_tag="ACG8KQ_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP9105417.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACG8KQ_002519"
     CDS             2873..3673
                     /locus_tag="ACG8KQ_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9105418.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACG8KQ_002520"
     CDS             3692..5560
                     /locus_tag="ACG8KQ_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9105419.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACG8KQ_002521"
     CDS             5553..6044
                     /locus_tag="ACG8KQ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9105420.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACG8KQ_002522"
     CDS             6032..6586
                     /locus_tag="ACG8KQ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9105421.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACG8KQ_002523"
     CDS             6604..7599
                     /locus_tag="ACG8KQ_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9105422.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKCE010000124         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000124 DAZKCE010000000
VERSION     DAZKCE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.126"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8KQ_002524"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8KQ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9105423.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8KQ_002525"
     CDS             1483..1935
                     /locus_tag="ACG8KQ_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105424.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8KQ_002526"
     CDS             1949..2638
                     /locus_tag="ACG8KQ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105425.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8KQ_002527"
     CDS             2763..5114
                     /locus_tag="ACG8KQ_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9105426.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8KQ_002528"
     CDS             5194..5589
                     /locus_tag="ACG8KQ_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105427.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8KQ_002529"
     CDS             5914..6531
                     /locus_tag="ACG8KQ_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9105428.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8KQ_002530"
     CDS             6572..6871
                     /locus_tag="ACG8KQ_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9105429.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8KQ_002531"
     CDS             6905..7072
                     /locus_tag="ACG8KQ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9105430.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8KQ_002532"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8KQ_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9105431.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKCE010000125         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000125 DAZKCE010000000
VERSION     DAZKCE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.127"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8KQ_002533"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8KQ_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9105432.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8KQ_002534"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8KQ_002534"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9105433.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8KQ_002535"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8KQ_002535"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9105434.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8KQ_002536"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8KQ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9105435.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8KQ_002537"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8KQ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9105436.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG8KQ_002538"
     CDS             complement(4433..5290)
                     /locus_tag="ACG8KQ_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9105437.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8KQ_002539"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8KQ_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9105438.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKCE010000126         6756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000126 DAZKCE010000000
VERSION     DAZKCE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.128"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..816)
                     /locus_tag="ACG8KQ_002540"
     CDS             complement(<1..816)
                     /locus_tag="ACG8KQ_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9105439.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            981..2711
                     /gene="spxB"
                     /locus_tag="ACG8KQ_002541"
     CDS             981..2711
                     /gene="spxB"
                     /locus_tag="ACG8KQ_002541"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9105440.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            complement(2897..3418)
                     /locus_tag="ACG8KQ_002542"
     CDS             complement(2897..3418)
                     /locus_tag="ACG8KQ_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9105441.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            3667..5289
                     /locus_tag="ACG8KQ_002543"
     CDS             3667..5289
                     /locus_tag="ACG8KQ_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9105442.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(5418..6281)
                     /locus_tag="ACG8KQ_002544"
     CDS             complement(5418..6281)
                     /locus_tag="ACG8KQ_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9105443.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT         2175 a         1350 c         1257 g         1974 t
ORIGIN      
        1 tcctaatgcc acatccaaaa tcatcataat gataaatcca ctcatgacgc caaaaactgc
       61 aaaatgacgt ttgctggaca atgtctgctg tgcttcaggg atcaattctt ccaccacgac
      121 ataaatcatt gctcctgcgg caaaagcaag tgcataagga agcagcagct ccactttcgt
      181 tactagtagc gctccaatca ctccagcaat tggttcaacg attccagaag cttgtccgta
      241 aacaaaggcc tttttacgac tcagaccttc ttgtcgcaac gggattgata cagctgctcc
      301 ttctgggaag ttttggatac caatacctag cgccaccgaa acagcggcta atatagcggc
      361 tgtcggatta tccgcagttg ctgctgcacc gaatgctacc cccacggcta aaccttcagg
      421 aatgttatgc aatgtaatag aaaaaacaag caagatcgtc cgttttaaat gggtaggcaa
      481 tccttctgct tcatgttgtg gtccaaaatg catatggggc agtgttttat ctgccatata
      541 taaaaacaca ccgcctaaac caaacccaat acttacaact agccaagcaa tatctccatt
      601 ttcttctgct tttgtaatag ccggatctaa caaagaccaa aaacttgcag caatcatgac
      661 accagaagca aatcctaaca tcaagtttag cacttctttt ttgatttctt taaagaaaaa
      721 aaccaacccg gcaccaagag ctgtcataaa ataagtaaaa gctgtcccag caagtgcttg
      781 ctgccaaggt tcaagtctca atagccattc aaccatgcca ttcccctctc ttgaatctat
      841 ctatgtacta ctctagcata ttcccaaaaa catgactaca tttttgtgaa aagacgaatt
      901 agttgggatt ttcgataaaa agttctatac tctagataca aaaaatttat gtatcatcat
      961 tgctaaagga gtgaaacacc atgtcaaaaa tcaatgcagg aatcgcaatg gtaaaagttc
     1021 ttgaaagctg gggtatcgac catatttacg gcattcccgg aggatctttc aattcaatta
     1081 tggatgcgct atatcaagaa aaaaccgaaa tcaactacat tcaagttcgt catgaagaag
     1141 tcggcgcact cgcagccgct gcagatgcaa aattaacggg gaaagtcggc gctgtcttcg
     1201 gctctgcagg acctggtgcg acacatttga tcaatggttt gtatgatgca cagatggacc
     1261 atgtgcctct agtagcgttc ttaggacaag ttgcttctac atccatgaac tataactctt
     1321 tccaggaact aaatgaaaat cctattttcg cagatgttag cgtatacaat cgtacagtca
     1381 tgacaccaca aagtcttccg catgtagtag atgaagccat taaagccgcc tatgagcata
     1441 aaggcgtggc tatcgtgact atccctgttg atcttggttt tgaagaaatt gacgaacaac
     1501 ctttctccac cgctcacact cacaaaacaa gtgtgatatt accagaagaa aaagatttgc
     1561 tagctgcttt gccttatctt gaaaaagcaa aaaaaccagt tctttatgtt ggtcaaggaa
     1621 cgcgaaacgg atttccgcaa atcaaagaat tctcagaaca tttttcagtt cctgttgtcg
     1681 cttccgttct agcaaaagga attattcctg atgattatga aaactttctt ggttttgctg
     1741 gacgtgtcgc tactaaaccc ggaaatgaag cattggcaga agccgatctg atcttatttg
     1801 tcggtagcga cttcccattt ggacgtgcat tttttaatcc aactgctgaa tttatccaaa
     1861 tcgatatcga tgcgtcaaaa tttggacgac gccatgaaac atctttgtcg atcctcggtg
     1921 atgcaaacac agctttgcaa agacttgtcg agttagggga tgctcgccct gctgatagct
     1981 ggtacctagc aaatcaagaa aataaagaaa aatgggtaaa ttggttgaaa tcttttgaag
     2041 atcgagagga agaaccgatt cgtccagagg ctgtctataa agaaatcaat cgtattgctg
     2101 aagacgatgc gattttcgtc acagatgtcg gtaacacgac tatccattct attcgtttat
     2161 tgaatatgaa cggtaaacaa aaacatacga catctggatg gttcgcaact atgggtaatg
     2221 gtgtgccagg aggaattgcc gcacaactaa gctacccaga aaaacaagta tttaccttaa
     2281 gtggtgatgg cgggtttgct atggtcatgc aagatatcat tacacaagtc aaatatcagc
     2341 ttccaattat taatgttgtc ttttcgaatg attcttttgg tttcatcgaa gcagaacaag
     2401 aagatacgga acaaaagaaa tttggtgtat ttttagaaga tgcagatttc ggtaaagttg
     2461 gagaagcgct cggggcaaat agctttacag tcacagaata ctcgcaactt cgatttgcct
     2521 ttgatgcagc tgcaaaatca gatcgtcctg tagtaatcga tgtgaagatc aataacaagc
     2581 gcccattgcc agtagaagat ctgcatttag atccaagaaa atattctgaa gaagaaatcc
     2641 aagcattcaa agaaaaatat gaagtccatg atttgccagt attgaatgaa ttatatgaaa
     2701 agaggttgtg acaactgcgc tatgcttcga gtattaagat aaaatttgtg tatatagctt
     2761 ctcacatttt tgcataaatt tgtctcgaca cctaaaaatc acctcgtgca tatttgtcca
     2821 tgataaaaaa gccggcttcc aattacaaca gtaaaactgt aatcggaagc cggctttttt
     2881 atttgtttga ttttacttat ctaaatgcat agacgatatg tgtgatacca aataacataa
     2941 aatagaatcc aactaaaaag cttaatgtca acgcagaaga aattggatta aacaataaga
     3001 aaattcctaa taaaattcct aaaatattga cgatgatcgt aaaccaataa tatccttcac
     3061 ttactcctct tgccaagtca gcagtaaata acgctaaaac tgaatcagca ataaaccata
     3121 ctgcaaaaac aaaaggcaat gctgcgactc ctgcaccaat attgaataag aaaaagacgc
     3181 caactagaat atcgatgatc ccgattatta agggcatctt tcctttatat cctgttagct
     3241 cttttaatct gttccgtaca aacagttcaa atatcccttt aataaaggca aaaatcgcaa
     3301 agactactac aattgcaact aaatttccga cagggtcttg aaatgataac aatgagacaa
     3361 gtacaaataa aattcccaaa atcaatgagc cccagtcgat ggttcttttt tcatccattt
     3421 tcaatccttc tttcatttct tttacagttt tatagtactc cctcttataa ataacgtcaa
     3481 aaattatgct gttgcaaact aataaatcct atagtagcta ttttcgctcc gcttttattt
     3541 ttattttttt cattctgttt cacgtgaaac acaaagaatg ttcgattgcc ttgaatcttt
     3601 tacctttccc ctatataatt ctataagact tgtcaaaact aataatgata aaagtgagga
     3661 aaaaaattga ttactgtaaa tgatgtgagt ttgcactttt cagaccgcaa actatttgat
     3721 gatgtgaata ttaaatttac ccccggaaac tgctatggct tgattggtgc aaatggtgca
     3781 ggtaaatcaa cgttccttaa agtgttatca ggggatctgc agccttccac aggttccgtt
     3841 actatgggac cagatgaacg gatggccgta ttgaaacaga accattacga ctatgaagac
     3901 tatactgtac ttgaaacagt catcatggga cacaagcgtc tgtatgaagt gctgaaagaa
     3961 aaagatgcga tttacatgaa agaggatttc actgacgaag atggtattcg agcagctgaa
     4021 ttagaaggag aatttgctga gcttaatgga tgggaagctg aaccagaagc agctgtttta
     4081 ctgcaaggtc tgaacatccc tgaagaattg caccatcagc aaatgagtga attaactggt
     4141 ggacaaaagg taaaagtgtt attagcacaa acactatttg gtaaacctga tgttcttttg
     4201 ctagacgaac caacaaacgg tctggatatc caatcgatca attggctgga agaatttttg
     4261 atcaattttg aaaatactgt gatcgttgta tcccatgacc gtcacttttt gaacaaagta
     4321 tgtacacata tggcggatct agattttggt aaaatcaaac tttatgtagg aaattatgat
     4381 ttttggttgg aatctagtca actagcggct aaacttcaag caaacgcaaa tgccaaaaaa
     4441 gaagaacaaa tcaaagaact ccaagaattt attgcccgct ttagtgcgaa tgcatcaaaa
     4501 tcaaaacaag caacttcacg taaaaaaatg cttgaaaaaa ttactttaga cgatatccaa
     4561 ccttcttcta gacgttatcc attcgttgga ttcaaacctg aacgtgagat tggaaacgac
     4621 ttattgcagg tagaaaatgt aagtgtaacg attgatggaa agaaaatttt agatgatata
     4681 tcttttaatt tgagcaaaga agacaaggtc gctttcgtcg cagaaaatga tataacaaca
     4741 acaacgctat tcaaagttat catgggtgaa attacgccag atactggaac agtacgttgg
     4801 ggtgtgacaa caagtaattc ttatttacca aaggatacaa cgaaagaatt cgatactgat
     4861 ctaacgattt tagactggtt gcgtcaatat gcaagtaaag aagaagatga taacaccttc
     4921 ttaagaagct tcttaggaag aatgttgttt tctggtgaag aagtattgaa accagtgaat
     4981 gttttatctg gtggagagaa agtccgttgc atgctttcaa aaatgatgct ttcgaaagct
     5041 aacgtacttg tattagatga tccaacgaac catttagact tagaatcaat cacagcattg
     5101 aatgacggct tgatggcatt tactggctca atcttatttg cgtcacatga ccatcaattt
     5161 atccaaacat tggcaaaccg catcattgcc gtttcggata aaggtgtgat cgatcgagct
     5221 gatacgacat atgatgaatt tttggaaaat gcagatattc aaaaacaatt gaacgagtta
     5281 tgggcataat ggcctattta gcttaaaaaa taaaaaaaca gcgacctcta agagatagga
     5341 tgctcatcta tcttttaaga gtcgctgttt ttgttacaat caatttctgg gcttttttac
     5401 agaatgagtt actgctttta tcgactgatt cccatcgaaa aaatcgcgat acagccatag
     5461 cctgtgtgac tggcgatcgt tggtcctaag ggataaagct tcacatcttt catctttatc
     5521 tgctcttcca aaagccgttt tgccttctcc gcgctctcta gatccccagc gtaagccaca
     5581 taaacgattt gatcaagtgg tgcaacgatt gtttttaccg tctcattgac gattgtctgg
     5641 atcgacttgt ttcttcctcg aactttgtca acattcgtca gccttccttc tttatccacc
     5701 gtaataatag gctttacact caatagactg ccaagagtag cacttgcttt ggaaatccta
     5761 ccgcctcgtt ctagatattt cagatcgtct actgttaccc atgaatggac tttcaaataa
     5821 ttactttcca gccactctag ggtttcttcg atgcttttac cttctttttt caatcttaca
     5881 gcttccaata ctaaaagccc ttccccaata cttgcagctt ttgtatcaaa aacatagatc
     5941 tcagggtctt catattcttc cttcagcaat tcaaccgctt gaagcgcact actgtaagag
     6001 ccgcttaatc cactggaaaa agccacatat aaaacaggaa tctgtttttc cgcataagaa
     6061 cggaaaaatt ctaaatatcg gccaacattg atttgtgatg tcgtaggcat cgcaccattt
     6121 ttcagctgat tcatgtagcg gtcataattg aatgtctttc cgaggtcatc tactaactct
     6181 tgatcattca attggagatt cattgataaa aaatcaactt tttcttttgc taatacatgg
     6241 tatggcagat cacaacaaga atctgttaca atttgataca catttttcaa ccccttctcg
     6301 ttacatttta cccttatcgt ctattatagc aaatctcttg cgctagacac actttaaccg
     6361 gtttattttc gcaagttcta atcaaaataa gaggttgtga cagaagtggt cagcttcaag
     6421 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc
     6481 cgtagaagct acttctggaa caccgtgaat cagaggttgt gaaatcagcg ttttgacctg
     6541 agtattagag aaaaaaacgg aaaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     6601 accgagggtc agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttagctc
     6661 cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata ttgtgacttg
     6721 tgaccgagga gttggcagga gaacaccgtt tagtgg
//
LOCUS       DAZKCE010000127         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000127 DAZKCE010000000
VERSION     DAZKCE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.129"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8KQ_002545"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8KQ_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8KQ_002546"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8KQ_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9105444.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8KQ_002547"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8KQ_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8KQ_002548"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8KQ_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9105445.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8KQ_002549"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8KQ_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9105446.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8KQ_002550"
     CDS             3582..4271
                     /locus_tag="ACG8KQ_002550"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9105447.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8KQ_002551"
     CDS             4285..5787
                     /locus_tag="ACG8KQ_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9105448.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8KQ_002552"
     CDS             5800..6276
                     /locus_tag="ACG8KQ_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9105449.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8KQ_002553"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8KQ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKCE010000128         6410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000128 DAZKCE010000000
VERSION     DAZKCE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.130"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8KQ_002554"
     CDS             complement(99..1229)
                     /locus_tag="ACG8KQ_002554"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9105450.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8KQ_002555"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8KQ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9105451.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8KQ_002556"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8KQ_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9105452.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8KQ_002557"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8KQ_002557"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9105453.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8KQ_002558"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8KQ_002558"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105454.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8KQ_002559"
     CDS             4880..5692
                     /locus_tag="ACG8KQ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105455.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8KQ_002560"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8KQ_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105456.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1921 a         1312 c         1007 g         2170 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa
//
LOCUS       DAZKCE010000129         6354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000129 DAZKCE010000000
VERSION     DAZKCE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.131"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(489..863)
                     /locus_tag="ACG8KQ_002561"
     CDS             complement(489..863)
                     /locus_tag="ACG8KQ_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9105457.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="ACG8KQ_002562"
     CDS             complement(886..2286)
                     /locus_tag="ACG8KQ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFP9105458.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="ACG8KQ_002563"
     CDS             complement(2290..2700)
                     /locus_tag="ACG8KQ_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9105459.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="ACG8KQ_002564"
     CDS             complement(2718..3209)
                     /locus_tag="ACG8KQ_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9105460.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="ACG8KQ_002565"
     CDS             complement(3219..4010)
                     /locus_tag="ACG8KQ_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9105461.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="ACG8KQ_002566"
     CDS             complement(4003..4776)
                     /locus_tag="ACG8KQ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9105462.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4932..>6354)
                     /locus_tag="ACG8KQ_002567"
     CDS             complement(4932..>6354)
                     /locus_tag="ACG8KQ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9105463.1"
                     /translation="LRDRILKECAGSSEYNYLESDEFIALRNYVVNKISPIIDSTGLC
                     LLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEESK
                     QELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIE
                     GETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALA
                     IECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDIL
                     KRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLING
                     NQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKY
                     YDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKD
                     IYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         2041 a         1262 c          822 g         2229 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc atactctcct
     4981 ttgggtattt caatacagta aatatctttg atttctgaaa aaaaatcttt tgatacattt
     5041 aaaaaagtat caaatagttg cgctccctct ttcaaatgtt catttgttat tccatcacca
     5101 atcataattc gttcaatcat tccagaaaga tgtagaaata gattaattct cagaaaactt
     5161 gggattttga catcgtaata tttttcatac aaatatagta ctttttctat ctcattgaat
     5221 acttttttgg aatctaaaaa gcgaagctta gtactagcgc cttcaatagt aaagagttta
     5281 actaattcat ttaagcagaa ctcacagcta tccgagtcca tatacttatt taatttttct
     5341 aaggtttgat tcccattaat tagattttct aaatttaaac aatcgattcc ttgaatgtaa
     5401 aattcagtgg ttgaaagaac acaaattgta ttcttaaaaa tcatagtatc tttttccttt
     5461 gctattttct ttaatgcgtt aaaatcaaaa atcaaaatct ccacttgatt atcgagatac
     5521 cgttttaaaa tatccttaac tttttcagaa atcccctcac cagagagaca agatactatt
     5581 acatttttct tttgtgataa tcctttataa tactgaactt taacttgaaa cgaatcaaaa
     5641 tccatctggt aaaaatgact aattggcttt ttttggcaaa cttgaatccc acattcaatc
     5701 gctaaagctg tagaaacatt gtttaaaatt accaaatcac ctattacatt ggattcaatt
     5761 ttttcataca tttgttccaa tgatcccata tcaactagca gaatgagtcc attcgtcgta
     5821 tctatacttt tacaaaaatt gttgattagg ttaattaagt cactagtctc accttcaatt
     5881 ggcatatcga aagcctcata aacatatgta ttcagcaact tatttgtttc ccttgcgatc
     5941 gcagaagctg ttattttacc gtgcataact atcagacaat gggtttctgg tattttcaca
     6001 tagaacttct ttacaagaaa aagtatccat ttcaacaaaa gttcttgttt actttcttcg
     6061 acttctaaac tactccaaat ctcatttgcc aataattttt gtttatcatt tttcatacga
     6121 aattttctga acttagacag caaaaaattt cttgatgttt cttcaattac atcaataaac
     6181 attataaata gactgatatc cgccaagaat tcatctaata aacatagacc tgtcgaatca
     6241 ataattggtg atattttatt aacaacatag tttcttaatg ctataaattc gtcactttct
     6301 aaataattat actcagaaga acctgcacac tctttaagta ttctatctct taat
//
LOCUS       DAZKCE010000130         6173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000130 DAZKCE010000000
VERSION     DAZKCE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.132"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8KQ_002568"
     CDS             725..811
                     /locus_tag="ACG8KQ_002568"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9105464.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8KQ_002569"
     CDS             complement(932..1621)
                     /locus_tag="ACG8KQ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105465.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8KQ_002570"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8KQ_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105466.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8KQ_002571"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8KQ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9105467.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8KQ_002572"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8KQ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9105468.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8KQ_002573"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8KQ_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9105469.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8KQ_002574"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8KQ_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9105470.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8KQ_002575"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8KQ_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105471.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8KQ_002576"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8KQ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9105472.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8KQ_002577"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8KQ_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9105473.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZKCE010000131         6170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000131 DAZKCE010000000
VERSION     DAZKCE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.133"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..389)
                     /locus_tag="ACG8KQ_002578"
     CDS             complement(63..389)
                     /locus_tag="ACG8KQ_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105474.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(435..797)
                     /locus_tag="ACG8KQ_002579"
     CDS             complement(435..797)
                     /locus_tag="ACG8KQ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9105475.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(834..1048)
                     /locus_tag="ACG8KQ_002580"
                     /pseudo
     CDS             complement(834..1048)
                     /locus_tag="ACG8KQ_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1176..1511
                     /locus_tag="ACG8KQ_002581"
     CDS             1176..1511
                     /locus_tag="ACG8KQ_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105476.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1587..3584)
                     /locus_tag="ACG8KQ_002582"
     CDS             complement(1587..3584)
                     /locus_tag="ACG8KQ_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105477.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3682..3957)
                     /locus_tag="ACG8KQ_002583"
     CDS             complement(3682..3957)
                     /locus_tag="ACG8KQ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105478.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4189..4923
                     /locus_tag="ACG8KQ_002584"
     CDS             4189..4923
                     /locus_tag="ACG8KQ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105479.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            4962..6104
                     /locus_tag="ACG8KQ_002585"
     CDS             4962..6104
                     /locus_tag="ACG8KQ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9105480.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2097 a         1135 c          890 g         2048 t
ORIGIN      
        1 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
       61 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
      121 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
      181 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
      241 tactgttctt gtaaaatctg tgttaccaat ggtttcgatg acttctctaa tccagatgta
      301 cctgtcattt ttgaaaaaat gatacctata atagccaaga caacgataac tataatgata
      361 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgc
      421 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
      481 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tatctcgata taagtagccc
      541 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgctac taccatagaa
      601 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
      661 atttcattaa ttgtctgctc agtacttttc tttactgcgt ctgctccagt tgtctcatct
      721 gtttcttgta gtgctccgca aaactggcaa tacttacttt catctgttat ttctttgcca
      781 cattttctac aatacatgtc ttctcctcct aaaaacattc aattgtttat tagttaactg
      841 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
      901 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
      961 tgctgtgtaa cattcatttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt
     1021 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
     1081 atatgatgta tttcaactca taaaatataa aaaaacttca aataccatta caaatacaac
     1141 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
     1201 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
     1261 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
     1321 gcttaaccgc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
     1381 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
     1441 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
     1501 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
     1561 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
     1621 actctccgtc catcatctct tgttacactt gtaaattgat aaagaggaac taattcaact
     1681 cctgataata tcaattcttt tattacttca tctatccctg ctccttttgc taggtagact
     1741 aaaaaactac tacttgcttt cacattgtat agtctcttaa tatccttaga agctcgttca
     1801 agagtgaatc cttgtttaac aaagtagcct ctgttttctt ttaaagcatt ttccacgctg
     1861 ttattcccaa cattacgtag acgttcacaa acagtgttag taagttcttt ctctgtcatt
     1921 agatatgttc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
     1981 tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
     2041 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt
     2101 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
     2161 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
     2221 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
     2281 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
     2341 ccatagtaaa aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
     2401 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
     2461 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
     2521 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
     2581 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
     2641 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
     2701 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
     2761 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
     2821 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
     2881 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
     2941 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
     3001 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
     3061 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
     3121 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
     3181 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
     3241 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
     3301 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
     3361 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
     3421 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
     3481 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
     3541 tctttatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
     3601 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
     3661 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
     3721 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
     3781 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
     3841 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
     3901 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
     3961 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
     4021 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
     4081 tcgacataca cataatacct cgataaaaaa gaggtgtcaa tatctttgta aaatttattt
     4141 acaacacccc tttttcgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
     4201 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
     4261 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
     4321 gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
     4381 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
     4441 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
     4501 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
     4561 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
     4621 aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa
     4681 gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc
     4741 agtatagaag aactactaga aagaatggta gatgaatggc tacctattca attgaaagac
     4801 atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa
     4861 acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc
     4921 tagtggtaag tactccttgt aaactaagag gagatgattt catggcaaaa atatcaaagt
     4981 acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac
     5041 gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa
     5101 aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata
     5161 tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt
     5221 caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc
     5281 ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta
     5341 aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg
     5401 caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt
     5461 atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta
     5521 aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga
     5581 gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac
     5641 atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa
     5701 caagagcttc tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact
     5761 ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc
     5821 aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt
     5881 taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata
     5941 cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag
     6001 ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta
     6061 agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat
     6121 ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat
//
LOCUS       DAZKCE010000132         5970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000132 DAZKCE010000000
VERSION     DAZKCE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.134"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(432..788)
                     /locus_tag="ACG8KQ_002586"
     CDS             complement(432..788)
                     /locus_tag="ACG8KQ_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9105481.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(802..1212)
                     /locus_tag="ACG8KQ_002587"
     CDS             complement(802..1212)
                     /locus_tag="ACG8KQ_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9105482.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1558..3744)
                     /locus_tag="ACG8KQ_002588"
     CDS             complement(1558..3744)
                     /locus_tag="ACG8KQ_002588"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9105483.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3962..4645
                     /locus_tag="ACG8KQ_002589"
     CDS             3962..4645
                     /locus_tag="ACG8KQ_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9105484.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4726..5445)
                     /locus_tag="ACG8KQ_002590"
     CDS             complement(4726..5445)
                     /locus_tag="ACG8KQ_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9105485.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5757..5963)
                     /locus_tag="ACG8KQ_002591"
     CDS             complement(5757..5963)
                     /locus_tag="ACG8KQ_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105486.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
BASE COUNT         1655 a         1148 c          944 g         2223 t
ORIGIN      
        1 ttttgatttc gtttttcgaa atcttacagc aactcattta tcataactta tcatttgtta
       61 tttgtcaaga acttttttga agtttttttc gatagaatca agcttcacaa gtggttcttt
      121 tttaacaact cagttatctt atcatgtcat ttctaacatg tcaacaacta ttttatatgt
      181 tttttaaaat actattttat tgctttgcta cttaaaacaa cagaaatgaa tatatcatgt
      241 aaatttacac atgtcaatat atttttaact ctatatcccc acctagattt gaattttgac
      301 cacataaatt gagcatgtct ctatagtaga aaaataaagt caaaaaaaga acacataaca
      361 cgcttgatgc taagtgttcg actgttacaa ctgtatgaaa tgctcagtga cttgattaaa
      421 acagttaaag tctatttatt taagataagc tgttccatca actgctcatt gtatccaata
      481 aatagaactt catcgataat aatcaaagga cgtctgagga gattggtatg ttgtgtgcat
      541 aaaaaaatca gttgtttttt tcctacacag gaaaggtcaa tcgcaatgtt gtgaaaagcc
      601 tcagaccaag tcgcaaatag ctgttctgct tgcacttctt caaaactgca taattcttct
      661 gcttcttctg tacttagatc gcgttcttct aagtcaacaa attgataagg caattgatgg
      721 tgttctaacc attgtttcgc cttcagactt gttacacagt gcgctgatcc atagactttc
      781 acttccacga aggctccctc cctatgaatt taagatgtta ttgttttgta aatagatcag
      841 atttctggca acacatcgaa tttcacgagg gacaaatgac cggatctcat cttcattaaa
      901 tccgattagt attctttttt catcaatcaa aataggtcgt ctcaataatg aaggatattc
      961 tttgaataag acgatcattt gctttaaagt taattcgtta aaatctatgc ccagcttttc
     1021 gtaaattttc gaccgagtgg aaataatatc ttgaactcct ccttcagtcg ctgccaagat
     1081 ctttttgatc tcactttctg ttaacggttc ggcaaaaata ttcttctctt caaattcaag
     1141 ttgattcgct tgcaaccatg ctcttgcttt tctacaggaa gtacagctag gcgaagtata
     1201 tacttttatc attttctcac tcctttttaa ttataatcca attctacagt aatttctttt
     1261 aaaataccaa ttattagcta ataaaatata aaaaaaatca aaataactga ttatttacgt
     1321 ttgaataatg agaatttttc tatatattct ctattttgat aatagtattc tcattttcaa
     1381 aataaataag ggaggtgtga caaaattctt gtcacacctc ccttttccga ataaactgtg
     1441 ttccagaaat agcttcttcg aaaataagct gaaatttcac aaaaatttga gaagcaattt
     1501 tcgtacattc cttcttcttt cttgaaactg accacttctg tcacaccctc tcttctatca
     1561 ctttcctttt ttcacacgac tcccggtttc tttgataaat ctcgatactt tcaattttct
     1621 tgaatttttc atttgaggta cacatagata cagacgattt cttgctctag tcatcgcaac
     1681 ataaaatagt cttcgttctt cttctatcgt cgttactggt tctgctttta tttcattaaa
     1741 gtcgattact ttcttttctg tcgctccgtt ataaggcaaa cttccttcgt tacaaccaat
     1801 caaaaagaca tttcggtaac taagaccttt acttttgtga atgctctgga tacatagtgc
     1861 atcaagtttt ccttgtctgt aagcaatcaa gcgggttttt aattctttag ctgtcgcttg
     1921 ggtttcttgc gataaggctt ggagtcttct gaaagtagca tgctgcttta cttcttctcg
     1981 tatccattct tgatcactct ggtcgatttc cttagctata cgtggcagat gaagatagct
     2041 ttgaacaagc gaatagattt cttgatgctg gtctgtttta cgcaacgctt cctcaaactc
     2101 tcggatttct gcagatttcg attttaaatt ggggcttatc gacaaaatat aatctgcaac
     2161 ggctatattt tgatatttag gagaaaagta ttgatcgcat tcactttttt tcaggtagca
     2221 gtctgccatc agctgccgga gcaattgctg gtaggcctga tttttctttt gcagattcag
     2281 tgctgtttgc caatgaacaa ttttttgata gaaatgttgg aaagacttga taccaaagat
     2341 accttcatat ggactatctg cttccagtac aaaaggaata ttcgttttca gcagagattg
     2401 gacaagctgc tgcgtttgtg aagctgatcg gtataatatg attgattcct tatatttccc
     2461 atttttttga ataaatgtct gaatttgttt gattagccac tgactttctg ctttagtatc
     2521 agaaaacaaa taggcttcca taacacctgc ttctttttgt gctggaatca tcgcctttgg
     2581 tatccgatca tttttattat agctgatcaa tctatttgct ccttggacaa tagctgcccg
     2641 agaacgataa ttagttaata agaaaagagt tttggtagga tattcttttt caaagttctt
     2701 gatgtatgct ggctctgatc ctctgaatcc atagatactc tgatcatcat ctccaacaac
     2761 aatcaacttg atcctgtttt cctttgtcat ccgtttcacg atttcccatt gaagtggatt
     2821 agtatcttga aattcatcaa tgaatagata atcatatttt tggccgatat aatgctggat
     2881 ctcctcattc tccaaagcgc ttttcagatt ttggagtaca tcattgaact ctagcaattg
     2941 ctggcttctt ttttgttctt ggagttccgt gagcaatcca tacagtcgtt ctgatatcgg
     3001 ctgtcgtgca ttcttatcaa tggaatcata tttttcttta actaactgaa ccatttcttc
     3061 tactgaaagg ttcatattct gccaatagtc taaccgagaa gcaatcgttt tttctccata
     3121 ttgatcaaat atataaagat tatatttacg tatcagctgt tgcaaaacag cattcacttt
     3181 ttgtgcagta tcctttgaaa agtctccttg tattccttgc attgctactt caggaaattt
     3241 tcgacaaatt tcattgtata tcctaaaaaa gatactatgg aatgttccga tatggacagc
     3301 ggtttttctc tggctgaaat tatctactcg tttttttagt tcatttgcag cgttccttgt
     3361 aaaagtgatc attagtatgc gttcttcgtc tacttgtttt tgcgtcatca gataaacagc
     3421 ttttgcacag attgtttgcg ttttgccgct cccagctcct gctatgattc gaagatgttt
     3481 cttcatatca tatgtgatag ctgctttttg ttcttgattg aagcgattat aggaacgttt
     3541 ctccatcatt ttaaagaaag agctgtttcc ttcactttct tttacttgta cttcttcctc
     3601 aataagtccg ggtgcttcgc cataagccgc ttcataggta gcaatgattt cttcttgtag
     3661 ccgaaagact tgttgaagct ttttatactt gttctcccag cgtttcaatt ctttttgtaa
     3721 tcggagaatt tctttttcat tcaactgatt gatttccttc ccatctttct tctttgaatt
     3781 tgtttatttc aagttggtta tttaaagttt cgttgtctat tttttcttta tactaacaga
     3841 cctttctaaa aatgaaaatg ggaagcgata tgattttagt tttctgaaaa tttttccttt
     3901 ttttcaatcg tttatgctaa gataaggaag acaaaaatat tcataaaagg tgatgaaaaa
     3961 aatgcagact tcctttcacg aggggcgtac aataggactt ttgctgacat ttatcggagg
     4021 agccatggac agttatactt atatccaata tggtgctttt gcctctgctc aaactggcaa
     4081 tatcattcta tcgatcatcc aagcatttga cggagaatgg ctaagtgttg gtaaaaaagt
     4141 attgtctacc cttttctttt tcttagggat tcttctaaca aagtatctaa ttgattattt
     4201 tagaaaaaaa gaaaaacatt tctggcgctt gtttgttcta tattatgaag cagtgatttt
     4261 tttctttgtt agtttgacac ccgtccatga gcatcccgca ttagtaacga ttttgattgc
     4321 ttttactgca tcgatccagt gggtagcttt tgataaaatc aacgggcgtg cctacactaa
     4381 tttatttaca actggaaatt taaaaggggt agcaacgaac ttatatgatt attgtatttc
     4441 taaagaaaaa gcagattttg atcgattttt ccactatcta agtgtcgttc ttgcctttat
     4501 cgcaggagct attgtccttg ttttctgtca tcatctgttt ggcgcccatt cgattttgat
     4561 cgtttccatt ttatttttga tcatggccct cactcagaca tttcatttat ggcaatttta
     4621 tcatacgaat cggttcacta tttgatctca atgaatcaaa aaaactaagc aagatgctgc
     4681 tcacagagaa atatctgcga gtgagcaatt tgcttagttt ttatattatt tagttgtaaa
     4741 gattcccttt gctttcgggt tatttgtagt gtagcggatc gactgggcat ctgcctcttc
     4801 tgtcattttc tttgcccgca tcttgatttc ttgtacgatt tgttctccac atgccggatc
     4861 ggctacgaaa tcatatttgt ccccatcgat cactattttg ggacatatat caaactgttc
     4921 ataccactct tcgtatcgtc gattgagttc tttatagtag gaatagagtt caggatcttg
     4981 ttctagctgt tcatattccc ggcctctttt tttgattctc gctaacatag tttcaaagga
     5041 tacacgaata taaaccagta gatctggttt cttttgtggc gaaatttctt ctaactcctt
     5101 cagcatcgtt tctaaaagac cttcatactg ctttacttct acttcagaaa cacgccctaa
     5161 atctgcattc aaatgaaaca gcatactatc ttcgtagatg gagcggtcta gtacgttgtc
     5221 atcatggctg aaagcatctt tgattgccaa aaagcgtttg ttcaaaaaga aaatctgtaa
     5281 taaaaaagta tacttttctg gattagaata aaatagcggc aatacttcat tatcttccac
     5341 gttctcataa aaaggttttg tttctaaatc ttgggcaagc atttcagtta gtgtgctttt
     5401 ccctgcaccg atcgttcctg caacaatgat cacaattatc ggcatccttc ctgtaataaa
     5461 atctgttctt cattttacta cttcatttat agaaataaaa gccttgactt tatctgctcc
     5521 ttaaaagcta cttctctaaa agtagccaaa agtaactcct attcatgaaa ataaaggctg
     5581 tgacaaaagt cttgtcgcag cccttttttc aaataaacag tattctcctg ccaacacctc
     5641 ggtcacaagg caatattcat aaaacatgaa aagctgtttt ttaacattat tccttgtaac
     5701 tcagagctaa acgggagact cacaacctct ttatgctctc aacattttat ctgtttttat
     5761 tcttctattg tacttgattt gcgtacaact aactgccagt gcagattatg agcggtatca
     5821 ttgtcttcta ctgcgcattt catgacacgt atcatcacac tattttcata gattttttct
     5881 atttttcctt ttattggtga ttcaaataaa tcagatttaa atgcatatgt ttcgccgact
     5941 tcaaacaatt gttcattttc cataagaatc
//
LOCUS       DAZKCE010000133         5750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000133 DAZKCE010000000
VERSION     DAZKCE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.135"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG8KQ_002592"
     CDS             14..1168
                     /locus_tag="ACG8KQ_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9105487.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1141..1584
                     /locus_tag="ACG8KQ_002593"
     CDS             1141..1584
                     /locus_tag="ACG8KQ_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105488.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2696..3664
                     /locus_tag="ACG8KQ_002594"
     CDS             2696..3664
                     /locus_tag="ACG8KQ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9105489.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3674..4162
                     /locus_tag="ACG8KQ_002595"
     CDS             3674..4162
                     /locus_tag="ACG8KQ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9105490.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4458..4739
                     /locus_tag="ACG8KQ_002596"
     CDS             4458..4739
                     /locus_tag="ACG8KQ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105491.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4741..5157
                     /locus_tag="ACG8KQ_002597"
     CDS             4741..5157
                     /locus_tag="ACG8KQ_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105492.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2148 a          982 c          937 g         1683 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga atcttttagg ggtctttttg tctgttttta ggggtctttt
     2581 tgtctgtttt taggggtctt tttgtctgtt tttaggggtc tttaattagg gggttgaccc
     2641 ccattagaaa gctctttata attttgttaa gtagttaatc ttgaaggagg attttatggc
     2701 aaatgaaatt gtgaaatacc accatgaatt aaatacgatt cctttacgca aatttacgtc
     2761 agtagaaatg aatttattct tctctataat atctagaatg cgtgatgtag gcgataaaaa
     2821 agtacaattt acattcgaac aattaaaaga tttaagcaat tataaagcaa ctgcaaatgt
     2881 tcgattcata gatgatttag aaacaacgta tgacaaatta atggatttac gttttggtag
     2941 aagaagtgct gacggtttgc aacgtgaacg atttgtttta tttaatcaat ttaaaatcga
     3001 tggaaaagct gatattcctt ttgctgaaat acaagtccat gagaaagcct tacccttact
     3061 taataatctt gaagaatggg ttcgatattc tctccaacaa ttcaatgaat tagaaagcag
     3121 ttattccaaa accatgttta gattattaaa acaatacaga accaaaggaa ttgccaattt
     3181 ttcaaaagaa gactttcatg aattacttga tatccctaaa agttatagag aaagtgatat
     3241 aaataaaaaa attataaagc ctattcgcca agaactaacc gcaatcttca aaggtttgac
     3301 aatccaaaag agatacggaa aaggacgggg aaagcctgtc attggctatc aatttacatt
     3361 taaacctgag ataaaaaaat cagacgactt taacaaacat gctccaacca aagaagaaca
     3421 aaataaagct ttggaactag ctaatcaaca ggttcaatct gaaaaagaac ctacttcatt
     3481 acaagaaaaa atcaaagaac aaaaacgaaa attagaactt ttacaacagt tagcagaatt
     3541 agaaagaaaa caaaataccc caagtaagaa aattaacaaa caaatagcag ataacaattc
     3601 cgatattacc ccagaattta aagaaaactt actagataat ctccaaaact tatttaaaga
     3661 ataaggggtt tttatgacag ataaaactat caaagaatta gcagatattc taggtgtttc
     3721 aaaagataaa gtgaagtatc aactgaggaa attacctagt aattttacct acaaaaaagg
     3781 aaatattacc tacctgaaaa aagaagcagt aacaacaatt acaaaggttt tatcaggtaa
     3841 tgaatcgggt aacttaccta gtgaattacc cgattcatta cctaccgatt acctactcaa
     3901 ggagattgaa actaagaata atcatattga agaaaaagat aggcaaattg aaaaacttca
     3961 aatagcttta gaaacgcagc aaaaattact cgaccagcaa caacaactca ccctacaagc
     4021 caatcaacaa atcaaaaaac ttcacgaaca actccagcta acctatgcag aagaatcttc
     4081 agaaaaagaa cccctttctg ttactgaaga aactcaaaaa ggacaaaaca aggagaaaaa
     4141 atggtggcaa ttctggcaat aacccaacaa tttttattca taaaaattgt tgggttactt
     4201 tctttttttg cttttacaag catagcgaca tgatctcacg tcgctatttt ttaaagagca
     4261 taaggaaacg cggtatcact ttcttacgaa aggtgctagg taggctgtgc ctaccacgtt
     4321 tctaagaaaa aaatttgcta tcactttttc gtcattgtgg atagcttatg tatagtgttt
     4381 cggcaaaaaa cagctataca aatactatat ttgtatgata tacataaatt atacacaaat
     4441 gcgaaaggaa ttcttctatg gacattttag ttcggaatat tgatccaaaa gttatttcag
     4501 aaatagatag acgttgccaa gaactcacaa caaaaactgg caaaaaatgg agccgtaatg
     4561 cgtacctaaa agttctaatc gaaaacgatt ttgaccaggc acttaggcat tacaaaaaag
     4621 atcaattcga tttattactc gaaaaattta tcgatataca agcaaaaaat actgaaacat
     4681 tgaacgacta tgttcataca aacaaccaac tcattgaaac acttatcgga ggaatataac
     4741 atgagaaaaa cttctgttca aaaacacgtt accctttatc ccagtcacat tgacgtttta
     4801 gattccatca tcgaaaaaaa tccagatatc aataactact cggaagccat tcgttttcta
     4861 tgtatgagtt atgaacctaa tactaatacg acaactacca gcaaccaagc aaaactaaat
     4921 gcgatgggaa aagaaatttc tattatgtcc gaaatcattg cagaatttgc tgatttatat
     4981 ctaaaagata ctggtgttct tatcggtaca aattctaaag tatatcaaga agtgaaagaa
     5041 aaaattgaaa atcaactcaa tccctcccga cgaaatcaac ccaaaaatcg tcaaaaagaa
     5101 accaatcaaa cattcacttc tagaaatttt gacccaacaa actttctaaa aagatgaaaa
     5161 agaaaccgaa aattttttct atttctcagt ttctttttta gggggcagtc ttccagcaag
     5221 atttgcaagt taccggtaac ttgcacaaaa aaagaaaaat ccgcttgtgc gaatccttct
     5281 ttttgcattt tggggcgctg ccccaatctc caaatatcgg ttatttaata gcgtggtaac
     5341 tcaaaaaaat agaacgtact ctgaaataaa aaaagactgc tctaacgagc agccaataaa
     5401 aaataatgag cgtttattct tgaaaaagca gcgcgcttat gaagcgctgc caaaaattta
     5461 ccatatttta tgaaaaataa caagtttcta aaaaggaatc ggttaagatt cacaaagaat
     5521 cgtaaacaga caatagcgga aaatcaagct tccgaacgta ccttttagct agaccaatga
     5581 aaaatcacaa agtgagcccc tgtatcggtt gatacaaggg ctacttggca tactcgacat
     5641 tatgtctact agctcgataa ataacgatta tgttcccaaa acaatatttt atgaactata
     5701 acaaaaattt gttatcgcat attgtatatt ttaattatag gtgtatatca
//
LOCUS       DAZKCE010000134         5713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000134 DAZKCE010000000
VERSION     DAZKCE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.136"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(178..1224)
                     /locus_tag="ACG8KQ_002598"
     CDS             complement(178..1224)
                     /locus_tag="ACG8KQ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9105493.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(1235..1507)
                     /locus_tag="ACG8KQ_002599"
     CDS             complement(1235..1507)
                     /locus_tag="ACG8KQ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9105494.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(1542..2450)
                     /locus_tag="ACG8KQ_002600"
     CDS             complement(1542..2450)
                     /locus_tag="ACG8KQ_002600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9105495.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(2582..3814)
                     /gene="pepT"
                     /locus_tag="ACG8KQ_002601"
     CDS             complement(2582..3814)
                     /gene="pepT"
                     /locus_tag="ACG8KQ_002601"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9105496.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(3829..4950)
                     /locus_tag="ACG8KQ_002602"
     CDS             complement(3829..4950)
                     /locus_tag="ACG8KQ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9105497.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(4947..5648)
                     /locus_tag="ACG8KQ_002603"
     CDS             complement(4947..5648)
                     /locus_tag="ACG8KQ_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9105498.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         1640 a         1229 c          984 g         1860 t
ORIGIN      
        1 ggtcaatttt ggtcaaaata tacacatagt tttttgactt tttttctcga cttatataaa
       61 tcttaaaaaa acaaaattga ttgtatgtat ataaaaaaag agccaaacaa acgtaaaatt
      121 atcttatcca taacgttttt ctttgatttg ataaaaaggg tagatcttat aaaagaccta
      181 cccttgattt tctttattct ctttttttac ttctattatt tcttctttga tcactcttcg
      241 ccgttcagca atcacataca caaatccata gagaaatggc aagataaagg aactgaaacg
      301 atacagtaaa agcgaagaaa ctgcgtcaac tgttccaaca actggtctga ataataacaa
      361 atagacaaat tcaaaggaac caataccagc aggcgtagga ataacccctg aaagcactac
      421 tgcaaagctg ataaacgaaa tggtcagtag aaaatctatt ttaggatggt tttcaatcag
      481 aatcaaataa ggaagtacat accaaaatac aagttttaac atattccata gataaatccg
      541 caatagcgca gtcctgtctt ggataatggt ttggaccgtt tcccttaaag agtagatctg
      601 ctgattgcat gtatccaccc atttccgcaa acgctcactt ttaaataact tattcgataa
      661 gctgacaaaa aatatttgaa cattcaagct gacggacatc gccaataaaa aggcaacaat
      721 cacgatcgtc aaaaggatac cagcgatgat aaaccagatc attgatggac cattagaata
      781 aaaaagagag aattggatga ccaagccagc taatgcataa gtgatcactg ccagtttgta
      841 cataatcata tggagtgctg tcacccctgc tccctgggat agcttcattc cttttttgtt
      901 ataaaaataa atctccgaga tcagcgttcc tgtaccaaac gaaacgattc ggtaaaacgc
      961 aatatagcta gaggtcaaga acccatcttt cgttgtaaaa ttcggcgcga aaggttcagc
     1021 aatttctttg attgagcgcc cttcagcgat ttggtaaact gtccctaata taacagccgc
     1081 tgccaaaaca acaacactgg tagacagcaa ttcggcaaag atatctgcaa atgattgatt
     1141 gattaaataa taaataatgg caaaaatcac aatcagcaaa agcagattca gaatgatttt
     1201 agttttcgtt cctttagacg acatctcctg ctccttaatg gaaaaaataa atgagaatca
     1261 aatcaacaag tacacctgca gcgaatccta tagtaaactt ctttttccga gttttatgat
     1321 ggaaaacttt tcgagagatc aatcctccca aaccaccgcc taacaggctg acaaatatca
     1381 aattattctc aggaacacgc cagccgctag ttctagcctg atgtttatca taccccatca
     1441 gacaaaataa gtaaaaattc acaatgaaaa ggtaaaacca caaaggattg tgtaacaata
     1501 catccattac tcttcttcca actttctgtt aactactatt tctaagcttt ttcttctttc
     1561 tttggtgaat aaactgttaa tccttttgat acaacagata attcaactgg aagatcatct
     1621 gtcgtgtctc catccgttcg agtgcccaca gatttttcct gcgcttggat tttcatgcgt
     1681 ttggctgtga aataagtaat gccggggatc gtatagaatt tgcctctcat gattctaggc
     1741 aaataaaaaa taaatcggaa aatatctaag gaaggtacga tcgttacatg aaattttcca
     1801 tcatctaggg tcgctgagtc gtcaaaattt gtaaaaccgc ccacggagtt agacatcgtg
     1861 actgtcaaca gttgggattt tcctttccaa tgctcctcgt ctgtttctaa atccaaatga
     1921 tattttttct tcttcagcaa taacttaaaa aacttcttga taaagatcca cgagccgtaa
     1981 cgctgttttt cttcttgact gatccaaact gctgtatctg ctaagattcc gattgtcagt
     2041 gtagagataa tgacttcatc attgacttga ccatagtcta ttttccgtac attgccttcc
     2101 aggatcacgt cagcagcttc ttcttttttg aggggaatct caagaacacg tgcaaggttg
     2161 ttgacagttc cacctggaag taatccgaca tggtattgag ataatgtttt ttggaacgct
     2221 tggacgatat gatgaatcgt gccgtctcca cctataatta ctaaagtatc agcttctatg
     2281 gcttctgctt tcttgactaa ttccgcatca tcaatcgatg ggccagtttc agctagttct
     2341 acatataatt ctggctgctt ttcttttaca taatcttgaa accagcgtgc taatttctca
     2401 ccctcatctt tccctgaact tttgttataa aaaactagca gcttttccat tctttacctc
     2461 ctatttactc ttcaaaaaag agggcgggac aaccaattcc attgtcctgc cctttagcct
     2521 tagacagatg acattccatc attgggacga acgccttgtc ataagctgtt ctttttcgat
     2581 tttaactctt tgtgttcaat tctgcaattt ttacaatcac gtctgttgct ttttccatta
     2641 cttgtaaaga aacaaattcg aatcgtccgt gcatattttc tcctccggca aatagatttg
     2701 gcgtcggaat acccatgtaa gagatttttg aaccatctgt tcctccacga actggttcga
     2761 tcactggaga gataccaagc tcgatcattg cttgtttcgc taattccacg atgctcatat
     2821 ctttttcaat gatttctttc atgttgtaat attgatcata catatctact ttgattcgtt
     2881 cttgatcaaa tcgctgattc aactcttctt ggatatgtgt gatttgtgct ttgcgtgctt
     2941 cgaatttttc acgatcatga tcgcgaatga tataactcat agaagcttct tcaggattcc
     3001 catccaatgc catcaaatgg aagaaacctt cgtatccttc cgttttttct ggaacttcat
     3061 cggctggaag ctgattatga aaatcgattg ctaactgtag agcattgatc attgtatctt
     3121 ttgccgtccc tggatgtaca tttttccctt ggatcgtaat atttgcttga gcagcactaa
     3181 acgtttcata ctgcaactca cctaaaggac caccatcgac tgtataagca aaatctacat
     3241 cgaattgctc aacatcgaat ttatccgctc cgacaccgat ttcttcatca ggaccaaaag
     3301 ccactttgat ggtaccgtga gggatagaag gattattgat caagatctcc attgcagtca
     3361 tgatttccgc aatccctgat ttgtcatctg ctcccagtaa tgtactgcca tctgttgtga
     3421 tcaatgtgtg gccttcataa tttttcaaat ttggaaaatc ttttgtattc aatgtatagc
     3481 gttcttccgc atctagtacg atcgtagact cgccatcata gttttcaatg atttgtggat
     3541 tgactcctac tgcattaaaa tctgctgtat ccatgtggga aataaatcca atggaacgca
     3601 cttcatgatc aacattgctt ggtaatgttc ccataacgaa tccgttatct tcattataga
     3661 aaacatcagc taagcctaac tcttctaatt cttttttcaa ttcttgcgca aaagcaactt
     3721 gtgtctgagt cgatggagtc gttttgcttg ttgcatcgga gcgtgtttct gttttgacat
     3781 atcgtaaaaa acgtggcaaa agattttcgt acatagtatt ctctcctttt aattaaattg
     3841 aaatgggttt gtatttgttt cagaaacgaa aaagttaata tcccaatttt cttcttgttt
     3901 ccaagattca aatttctcga taaatcgttg cttgcataac gattcaatat aatgaccggg
     3961 atcaattgca atcaacccag cgctttgcat atcatgagcc gtatgataat agatatctcc
     4021 cgtgatatag acatctgctc tttgggcgat tgcttgcgga taaaattttt caccgcttcc
     4081 accgcaaatc gcaatgcgct tcacagagga ttttgcattt tttggctgga ctattctcag
     4141 tccatctagt tggaacgctt cttttacttg ttctacaaat gcctctatag aaatttcttg
     4201 cggcaattca cctactcgtc ctaacccaaa catttcgact ggttcatcta tagcgaaaag
     4261 atcataagca ggttcctcat aaggatgagc tgaccgcatg gcttgaatca cttgtttttc
     4321 gatagtttcc ggcaaaataa cctcaacttt tgcctcttga acttgttctg tcttccctac
     4381 tttcccaatc gctggttgtg caccagcttc cggagtaaat cgaccgtgtc caatcgaggt
     4441 aaaacttgta ccggtataat caccttgtgt accagcacca gctgcagcta atacttcacg
     4501 cattttctgt gcatgatcca caggtacata aacggccagt tttttataat gaatctcatg
     4561 agtctttact aaataactct ccacttcaat accgagaagt tcacaaaacc agtcattcag
     4621 tccatcccaa ataatatcca tgttcgtgtg tgctgcatag acggcaatat catgtttcaa
     4681 caaatctgca tacatttttt cttgtggctg atcagtaacc agccgtttta caggacgaaa
     4741 gattggagga tgtttagcaa tcagcaaatc gatttttttc ttgattgctt cttcgaccac
     4801 ttctggacga acgtctaatg tcatcattac tcgttggatt ggcttatcca atgttccgat
     4861 atgcagtccg actgggtctc cttcttcagc tagccactga ggacaatagc tttcaaactt
     4921 ttggatgaaa gtgcgtccgc tgattgtcat ggctttaaaa cctcctcaat catttggatt
     4981 tcctgctcga tttgtttttg acgtgcctct tgattagctg cttgtttgag ctgttcacgg
     5041 atagcttttc tttgttgaag ctctcttgtc cattttgatt tgaagatctc acttttctct
     5101 tcaagcagaa atggcccgaa catcagctcc atttcattgt aagtaactgg tttttgttct
     5161 ttttcggcta caatgatttc atatattttt ttattttctt caagtatttt ttctgattgg
     5221 attttatact gattttcttg cagccaagat cgtaatgtac gctctccgat gtttggctga
     5281 aggatcaaac gttcttgtcc gcttaagcgt tgattcaact tgccagcttc taggatatct
     5341 cggatcagtg aacctcccat acccgcaatc gtgacagctg taataccatc ttgtggctgg
     5401 atagcatcta gaccatttgc aaggcgtacg gtgatacggt cttctaatcc atcttttttg
     5461 acttgcttac gtgctgattc ataaggacct tgaacgactt caccagcaac tgcatattcg
     5521 atttttcctc ttaatatcaa tgcaacgggt aagtaagcgt gatctgatcc aatgtctgct
     5581 aaacgagcgg attcagggac aaagtcaccc actgcggcta agcgctgaga taaatctaaa
     5641 tggttcatag cttcactcct tctagataat tatagcattt tccaaataaa ataaggagac
     5701 taggaataaa aaa
//
LOCUS       DAZKCE010000135         5681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000135 DAZKCE010000000
VERSION     DAZKCE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.137"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2144
                     /locus_tag="ACG8KQ_002604"
     CDS             <1..2144
                     /locus_tag="ACG8KQ_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFP9105499.1"
                     /translation="FPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGT
                     SAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNV
                     LWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGL
                     VFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYS
                     RSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASAT
                     LSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGA
                     AVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQ
                     LISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNR
                     LNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKT
                     AIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFS
                     GTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQR
                     LALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQ
                     ILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2113..3528
                     /locus_tag="ACG8KQ_002605"
     CDS             2113..3528
                     /locus_tag="ACG8KQ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9105500.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3561..4595
                     /gene="sppA"
                     /locus_tag="ACG8KQ_002606"
     CDS             3561..4595
                     /gene="sppA"
                     /locus_tag="ACG8KQ_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9105501.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4597..5118
                     /locus_tag="ACG8KQ_002607"
     CDS             4597..5118
                     /locus_tag="ACG8KQ_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9105502.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5115..5501
                     /locus_tag="ACG8KQ_002608"
     CDS             5115..5501
                     /locus_tag="ACG8KQ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9105503.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2037 a          774 c         1076 g         1794 t
ORIGIN      
        1 catttccttt tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga
       61 ttttaaaata ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg
      121 atttagaggg tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt
      181 gtatggctat tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa
      241 ttgcacatat attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg
      301 gaaacgtact atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt
      361 ttcaaaaaga atggacaggt attcttttat tacctatgcc tagagaaact tatattccta
      421 ttaaagaaaa agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat
      481 tagtattcca catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat
      541 attattttca aggtattctt gattttttca tccctaatca agcacgttca actttaaata
      601 taatttccat tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta
      661 gttatcttct tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca
      721 aacatgtgtt aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt
      781 ctcgttttct tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat
      841 ttttagatat tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt
      901 tgtttttaat cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga
      961 aaagctatga aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta
     1021 tcatagaaag tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat
     1081 ataaccgtat agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc
     1141 ttgataatat tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt
     1201 tatggctagg ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat
     1261 acaatgcttt actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga
     1321 aaatgcaaac ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag
     1381 aaaaatcgaa ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca
     1441 ctgtaaaaaa cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat
     1501 gtacaattcc ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa
     1561 ctcttgcaaa gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa
     1621 acattaatca cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc
     1681 aagagtcatt tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc
     1741 caccaagttt cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta
     1801 atcaacagcc attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag
     1861 ggcaacgaca acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt
     1921 tagatgaagc aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc
     1981 tcaatttaaa agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat
     2041 gtgatcaaat tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac
     2101 ttcgttttgc cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt
     2161 atgcagaata ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc
     2221 taccgttggg tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc
     2281 tttgctaaaa aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag
     2341 aagttacaag tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt
     2401 gttcataagg acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct
     2461 cagatagaac aagaaaacca acgtattact caacaaataa agatggctca gctatttatt
     2521 gaaagtataa gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat
     2581 caattaaaga gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa
     2641 ttaaatgatc aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa
     2701 attgagagtt cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat
     2761 aatcaatcat taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa
     2821 caactaaata atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc
     2881 ataaatgaac aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct
     2941 aaattagtta aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag
     3001 caagagctag aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa
     3061 caagagaaga accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta
     3121 aaagctcctg tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta
     3181 gccaaaggaa cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt
     3241 acagcacttt tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt
     3301 aaattggatc aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca
     3361 gaaaattcta ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca
     3421 actcaatcgt ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa
     3481 aaaacatatt ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta
     3541 agaatatagg aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg
     3601 gattattgtt actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat
     3661 caaatacaac cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag
     3721 aaacgttaga agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta
     3781 ttgcagatac aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt
     3841 taacacagct aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa
     3901 attcacctgg gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta
     3961 aaaagctaga tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact
     4021 acatttctgc tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg
     4081 gtgtcattat ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg
     4141 cgacgtataa aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg
     4201 aagagaataa agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg
     4261 tagcaaaagg aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt
     4321 atgatggtaa ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg
     4381 cattagattc cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa
     4441 atgacacaac tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc
     4501 taaaggcaac tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata
     4561 ctccaaaacc aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa
     4621 actttgccta attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat
     4681 ttaatttgta ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga
     4741 ctggaatctg acaaatccat tattactttt tatggtatag cgtcattagc tatttattta
     4801 acctattttg tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt
     4861 gggataagag tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt
     4921 gagtgtatat gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct
     4981 gctttcacgc ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt
     5041 attcgttcat tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat
     5101 ctggagagaa ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa
     5161 taacagggat tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg
     5221 attcaactac tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc
     5281 taagcgggaa tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca
     5341 taataggttt tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca
     5401 ttttatctat tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag
     5461 gtattattgc gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa
     5521 cgtgaaacga ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg
     5581 atggaagaca tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa
     5641 aaagacactc tctgattgtt gtattaatgg aaaaggggaa a
//
LOCUS       DAZKCE010000136         5585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000136 DAZKCE010000000
VERSION     DAZKCE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.138"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8KQ_002609"
     CDS             complement(488..1297)
                     /locus_tag="ACG8KQ_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9105504.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8KQ_002610"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8KQ_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9105505.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8KQ_002611"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8KQ_002611"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9105506.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8KQ_002612"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8KQ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9105507.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8KQ_002613"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8KQ_002613"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9105508.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8KQ_002614"
     CDS             4179..4913
                     /locus_tag="ACG8KQ_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9105509.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="ACG8KQ_002615"
     CDS             4897..>5585
                     /locus_tag="ACG8KQ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9105510.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DAZKCE010000137         5571 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000137 DAZKCE010000000
VERSION     DAZKCE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.139"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8KQ_002616"
     CDS             complement(<1..300)
                     /locus_tag="ACG8KQ_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9105511.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8KQ_002617"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8KQ_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8KQ_002618"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8KQ_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105512.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8KQ_002619"
     CDS             1706..1942
                     /locus_tag="ACG8KQ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105513.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8KQ_002620"
     CDS             1949..3901
                     /locus_tag="ACG8KQ_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9105514.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8KQ_002621"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8KQ_002621"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9105515.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG8KQ_002622"
     CDS             complement(4797..5489)
                     /locus_tag="ACG8KQ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9105516.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1813 a          971 c          902 g         1885 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca t
//
LOCUS       DAZKCE010000138         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000138 DAZKCE010000000
VERSION     DAZKCE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.140"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8KQ_002623"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8KQ_002623"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8KQ_002624"
     CDS             complement(580..1353)
                     /locus_tag="ACG8KQ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9105517.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG8KQ_002625"
     CDS             1497..1847
                     /locus_tag="ACG8KQ_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9105518.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG8KQ_002626"
     CDS             1828..2544
                     /locus_tag="ACG8KQ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9105519.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG8KQ_002627"
     CDS             2544..3992
                     /locus_tag="ACG8KQ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9105520.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG8KQ_002628"
     CDS             complement(4062..4571)
                     /locus_tag="ACG8KQ_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105521.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG8KQ_002629"
     CDS             complement(4561..5286)
                     /locus_tag="ACG8KQ_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105522.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKCE010000139         5313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000139 DAZKCE010000000
VERSION     DAZKCE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.141"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8KQ_002630"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8KQ_002630"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9105523.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACG8KQ_002631"
     CDS             complement(1298..2134)
                     /locus_tag="ACG8KQ_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105524.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACG8KQ_002632"
     CDS             complement(2318..3535)
                     /locus_tag="ACG8KQ_002632"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9105525.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACG8KQ_002633"
     CDS             complement(3684..3845)
                     /locus_tag="ACG8KQ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105526.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACG8KQ_002634"
     tRNA            3918..4001
                     /locus_tag="ACG8KQ_002634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5313)
                     /locus_tag="ACG8KQ_002635"
                     /pseudo
     CDS             complement(4241..5313)
                     /locus_tag="ACG8KQ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DAZKCE010000140         5287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000140 DAZKCE010000000
VERSION     DAZKCE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.142"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..287)
                     /gene="prgO"
                     /locus_tag="ACG8KQ_002636"
     CDS             complement(12..287)
                     /gene="prgO"
                     /locus_tag="ACG8KQ_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP9105527.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(259..1212)
                     /locus_tag="ACG8KQ_002637"
     CDS             complement(259..1212)
                     /locus_tag="ACG8KQ_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9105528.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1824..3317
                     /locus_tag="ACG8KQ_002638"
     CDS             1824..3317
                     /locus_tag="ACG8KQ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9105529.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3452..3748
                     /locus_tag="ACG8KQ_002639"
     CDS             3452..3748
                     /locus_tag="ACG8KQ_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9105530.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3849..4700
                     /locus_tag="ACG8KQ_002640"
     CDS             3849..4700
                     /locus_tag="ACG8KQ_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105531.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4721..4933
                     /locus_tag="ACG8KQ_002641"
     CDS             4721..4933
                     /locus_tag="ACG8KQ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105532.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1803 a          823 c          997 g         1664 t
ORIGIN      
        1 cttactatat tttaatcttt tgatcgtcta ttatatataa tccgttcctc gtcgtttaaa
       61 gtatcaacat aggcgtccat tgcaagtttg actatttcat acataggaat atcttttgca
      121 taactaagat cccgaatggt ttttagtacg ggaggatcta cttgaactgg ttttctttct
      181 ttcgcagtgt actttcgttc ccctaactcg acattaatat catctttaat tgaaatatct
      241 tcagcaccaa ttttcttctt atctttgttc gttggtaata atgccattta gtttaatctc
      301 ctttccaaga ggtaggatat gattggccat tgagtcagca tatttaggtg tataatcaaa
      361 tccttcaata tcaccatgtt gttcatagta ttcaattctt tcttctaatt ctgtaaagat
      421 gtctgcaaaa atagcccacc atcttcgatc tacataatca tttaattgga taccattctc
      481 accatacatt tctaaccgat ccgagccttt aacaatgctt ttaaaaatgt ttgattcacc
      541 atacttatca attgtttttt gatagtttgc gtgttgagag gaacgtcgca ctgagaaaag
      601 aacaggcaaa attccaatga tttgaagatg agactcttca aaattcgtga caatcggaac
      661 taaaacttta ttaataaaat cttctgttcc ttccattgcg taccgtttta attcttgcat
      721 tgcaataata taatctgcag cagttaagaa tgcacgaacc acattatctg ttgatggtgg
      781 gcagtcgata aagatataat cgaaattttt tctcaattct tctaaaggtt taatgaaggc
      841 caatgatctc ttacgacgat caggatattt ttcggacaag tattcattaa gttcttcaga
      901 tccaggagag ccagccataa tatataaatg tggacttact tgagtaatac ctgaaaccca
      961 gtcccctttt ttgattgctg cagacatgga tcgatcaaag ctagtaattt gatacgttga
     1021 tccaatgttg gaagtagtat ttctttgttg gtcataatca attagcaaca cgtctttttc
     1081 aaaaagttga ctagaaacaa tcgctaataa atcattgtct gtagtcttac caatgccgcc
     1141 ttttctacct gtaagcatta caacttttcc tagtttatct gatgttttaa atgcagtccg
     1201 taagttttcc atagttccct ccaatttcct atactaaata tatttagtat acttcatata
     1261 cttaatatat tcgatatatc taatatactc caaattgata agaattcaga ctttataagg
     1321 aatatagtgg atatactaaa tatatttgct atattcagta tatcagatat attcaaaatg
     1381 tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt gaaaagaggt aaacttaatt
     1441 ctatagaaac cacagataat tcactattta caaagtagca aaagcagtgg tactctttaa
     1501 acagaaaaat tacataaaaa aaagagacgg aaataattga ttttagcaga atcaattatt
     1561 tctgaatcac atggttagga catgtgttca cgctcgtctc aagaatatgt aagtagctac
     1621 caactactta tattaaaagt tgatcggata ccttttccga ttgcctttat tctaccaaaa
     1681 aaaggcgagc aactcaacag aagaacgttt atttagtgta cttaaattaa atattcttaa
     1741 acactgaaga gagaacacat actatggctg agattaaagc taaactgtgt tctctttttg
     1801 tttgagaaag gacgttacaa tcgatgttga gcgaaattta ttcagctatt cttaaaaacg
     1861 gcttacgtac atacaaatat cgaaacagca ccatgaaacc tgcaggtcat attgattcca
     1921 gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta
     1981 tggtcattac ttcggaagaa gctattttag agaacgaaaa gaaactaacg cattggacac
     2041 ctaataccta tcgctttggt acttatgcgg atgattatcg aacagtggtt aaaggtcatt
     2101 ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga tatcgattca aaagagaacc
     2161 atcaaggcga aattatttta gcctgtttag atcaagtggg ctacatgcca acgctaattt
     2221 tagaatcaga tcatggttat caagtttatt ttgtactaaa gacaccaggc tatgtcacaa
     2281 agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac
     2341 agttggctga aaaattgtcc ggagtggatc taggatgtaa ccacttcgga attgctcgat
     2401 tcccacaaga aaataatatt gtttttaaag aaatgaacta tcagtattct tttgctgact
     2461 ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa
     2521 aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata
     2581 tgttacttca taaagcaaac atcgtcggcg gagaaggacg tcttggtcga aataatgtga
     2641 ttttcacgct ttctttagct tattactctt caggttatgc attggatact tgcgaataca
     2701 atatgttcac attcaacgaa cggttacagg aaccgctatc tgaagcagaa ttagtaaaga
     2761 ttgtacgtag tgcttattct ggtaattatc aagcagcaag cagagaattc attattgaat
     2821 tgtgccagca atgggtttca ccaaccttaa aaaataatga tctgttcatt caacgacgtg
     2881 gctggtggaa atttaaaaag ccacgagatc aaagaaaata tagtcataaa aatgaatgga
     2941 aagaagatct attggcttat ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc
     3001 tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa gcgaacactt gataagatct
     3061 taaatgagtt acgagaggag aataaagtct tctacaaggt aacacgagga agaaatggcg
     3121 gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa
     3181 agagactaga ttattcaaca aggatcacag aggtatttgg actcaacgag agcttagtag
     3241 aagagacatt tagacaatta tcaaccccct tgaaccctct taaagaagtg aatctttttg
     3301 aagtagatac aggttgaaaa ataaaaggtg caaattgccc tatacaatat atctatgata
     3361 cttctgtagg tgttgctccc tttgcctagc tacgtgtagg tggtatactt ataactgcgt
     3421 atgcagtctt atgtatcatg gaggaagttg tatgtcgtta aaaaattatg tctattcaca
     3481 tcctgttaat gtgtttatca ttggtcgttt gggattatct gtggaagtgt tctgtgagct
     3541 ttatggtttt aagcagggga ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc
     3601 tttgccgatt gagtttcttc atgggttgtc cttagcttct ggggagacta tggatcaggt
     3661 atatgattgt ttgtgtgtgt tagagcagga gtatattgag tataaaatag ctaacgagtt
     3721 aagaaaaagg aagaagtata tccagtagta gtttttattt acttaaaaaa ttggttcatt
     3781 gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta aaggaaagaa tttagggaag
     3841 aaatcgaaat gaaatcggac gactggattg aacacacctg gcttcctctg aaaagaaata
     3901 gcaaacgaaa gaaattttgg gtattgcaat tatggccatt aggaaaactt tgtagttacg
     3961 gggcacgact tattcttgat ttaaccgttt actcgattgg agtatttgga acggcttatt
     4021 acttgataaa tgatcccaat aggattgcag catcagacat ttttacagtg ctaatgacgt
     4081 tgatttttgt catgctattg tctattttta ctcaattagt catgcaattt accaggcaaa
     4141 aattaacagc aggaaaactt ttcaaaagcc tattcgattt aattgttgta aatgcaattc
     4201 tagcgacttt aataaaacaa attgaacaat ctggaagaac aacaaaagga gtgaaggatt
     4261 ttttattgac gtttattcac tttgtaaatg gacacatgta tttgtttgaa gtgggtatta
     4321 ttctcttttt tgtcgggtat gtatccacga aacttattaa acgttcaatt gaaaaagaaa
     4381 ttgaaattga cctgattccg acacttcttg gttatgagta caatgttgat tttggaagat
     4441 atgacgaatt gatcaatacg gatatcgttc acacgagtat ccatgaatct actgttaagg
     4501 gcatcagatc tattttaaaa aatggccagg aaatgaaagc aatagatcga agaaaagaga
     4561 tctacaattt ttatgaagtc gaagcaagcc tacgatatgg catcggaaaa ttgtggttag
     4621 atggaagtaa tattcgtcaa aacaaaccat acattttttg gaacagtagt gatttagaac
     4681 ttccaatgaa agataaataa ttatggagaa tcgtttgaaa atgattcctc tatacaggat
     4741 tgtgcttata gttggcatgg agttaaaaac cttaaagaag gagcagttat tcaaagtgaa
     4801 agaacgtcat aatcagttac tatgtagtaa aacgggtacg atgaacattg aataccgaac
     4861 agaatattta gaattaacga atcaaactga ttttttgttg attcgaaagc aacaagagca
     4921 agaaaaaaat taatggctac ttatagccaa ttgattgatg aatgggaaca attcaaggaa
     4981 tctaaaattc cagaacgtct ctaaaagcct ttatttgtaa agagcataag gaaacgtggg
     5041 aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat tacgtttctc aaaaataaat
     5101 gtgggaacaa aatatttaaa aataggatag tgaaaggata gtgaaggata gtaaaaggag
     5161 ggtagtagga tagcaaaaag gataggctcc ttttcgctat cctttttaaa ttggaggaat
     5221 taatatggaa gtaagaataa gaaatgtgga tgagaaaatc gtaagagaaa ttgatcgaag
     5281 atgtgac
//
LOCUS       DAZKCE010000141         5208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000141 DAZKCE010000000
VERSION     DAZKCE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.143"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(284..850)
                     /locus_tag="ACG8KQ_002642"
     CDS             complement(284..850)
                     /locus_tag="ACG8KQ_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9105533.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(850..1806)
                     /locus_tag="ACG8KQ_002643"
     CDS             complement(850..1806)
                     /locus_tag="ACG8KQ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9105534.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1806..2462)
                     /locus_tag="ACG8KQ_002644"
     CDS             complement(1806..2462)
                     /locus_tag="ACG8KQ_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9105535.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2832..4850
                     /locus_tag="ACG8KQ_002645"
     CDS             2832..4850
                     /locus_tag="ACG8KQ_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9105536.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4920..5126
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1704 a         1016 c         1012 g         1476 t
ORIGIN      
        1 tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat gtgttcaatt
       61 tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
      121 agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac cgcagatata
      181 agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct gagtattaga
      241 gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt tttttctcaa
      301 tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc agcacattat
      361 atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct ttttctccac
      421 cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt cgtttcaata
      481 tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta tcactttttg
      541 ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct atcacttctc
      601 ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga cgctgttcta
      661 gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag acatgaccag
      721 tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc gttgcatcta
      781 cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa tgcaaagcag
      841 tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga agctatctct
      901 gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc tccacattgg
      961 tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga tctcttttgg
     1021 tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca ttaattgaag
     1081 gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca tcaaatggag
     1141 cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt tcatcatttc
     1201 taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat tatgtttgcg
     1261 taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga tcagttttga
     1321 agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc gctcgttcag
     1381 ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac cgactgataa
     1441 tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta tttcaactgg
     1501 cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat gaggccattt
     1561 ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt caggtgcaac
     1621 gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg caacagtccc
     1681 atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt tcccgccaaa
     1741 ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg aataaggaaa
     1801 agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt ttctctatac
     1861 actggataag tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat gatatcagta
     1921 ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat aaacatcatc
     1981 agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag cagaaatagt
     2041 aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact gtaacttgtt
     2101 tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt gatcaccggg
     2161 ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc tgcccttttt
     2221 ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt aagaggatta
     2281 ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc tctgacaatg
     2341 cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac gatccagccc
     2401 aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt tttatatttc
     2461 atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag cgaaaaaaac
     2521 ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc ttataaaaaa
     2581 aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag ggaaataaaa
     2641 tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac ttgaaaatga
     2701 acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca aagctcttaa
     2761 ataactttta ataaatatta agagaagtta taattattaa taatagataa attgaagttg
     2821 gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac
     2881 gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg
     2941 tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg
     3001 gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg
     3061 ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt
     3121 ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct
     3181 atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata
     3241 aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg
     3301 tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca
     3361 cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt
     3421 acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag
     3481 ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta
     3541 gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt
     3601 atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg
     3661 atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga
     3721 caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaatt
     3781 cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc
     3841 aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga
     3901 ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa
     3961 atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct
     4021 ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag
     4081 ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc
     4141 aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca
     4201 gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca
     4261 gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat
     4321 ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata
     4381 atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg
     4441 caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg
     4501 ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag
     4561 tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata
     4621 tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat
     4681 caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg
     4741 tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata
     4801 ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt
     4861 cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg
     4921 acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga
     4981 gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa
     5041 gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt
     5101 ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc
     5161 ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacag
//
LOCUS       DAZKCE010000142         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000142 DAZKCE010000000
VERSION     DAZKCE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.144"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8KQ_002646"
     CDS             137..748
                     /locus_tag="ACG8KQ_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9105537.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8KQ_002647"
     CDS             821..1750
                     /locus_tag="ACG8KQ_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFP9105538.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8KQ_002648"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8KQ_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8KQ_002649"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8KQ_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9105539.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8KQ_002650"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8KQ_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG8KQ_002651"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG8KQ_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9105540.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKCE010000143         5171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000143 DAZKCE010000000
VERSION     DAZKCE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.145"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..968
                     /locus_tag="ACG8KQ_002652"
     CDS             <1..968
                     /locus_tag="ACG8KQ_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9105541.1"
                     /translation="DYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFW
                     NYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELF
                     IIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLL
                     EYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKS
                     FGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSV
                     FFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            965..1684
                     /locus_tag="ACG8KQ_002653"
     CDS             965..1684
                     /locus_tag="ACG8KQ_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFP9105542.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1699..2784
                     /locus_tag="ACG8KQ_002654"
     CDS             1699..2784
                     /locus_tag="ACG8KQ_002654"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9105543.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2920..4452
                     /locus_tag="ACG8KQ_002655"
     CDS             2920..4452
                     /locus_tag="ACG8KQ_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9105544.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4630..4851
                     /locus_tag="ACG8KQ_002656"
     CDS             4630..4851
                     /locus_tag="ACG8KQ_002656"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9105545.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1793 a          552 c          995 g         1831 t
ORIGIN      
        1 aagattacta taaagtattc gctataattg aaaaattatt ctggatttca ctgccacttt
       61 gtttaattga aagattcatg gtcacctatc caactggaac aattgtagga gatatgatag
      121 gtggagtttt ttggaattat tcaggttgta atatgccatt gaatttaatt ttgtgtgtga
      181 cagttactgt tgttacaatc ggatatttca agggagaaaa aaatttcatc cattttattt
      241 tatgtgtttg tgcagcactt tttatgtctg cattagctga attgaaagtt tttattcctg
      301 aattatttat aatatttgtt gcaatagtat tcactttaaa agttgatttt aaagtagtga
      361 tcaagatatt atcatgcgta atacttgtgt cgatagtatt atcctttttt ataacaatat
      421 ttattacatt gaacgataat ggacaaagct atgctgataa tttcacaatt caaggtcttt
      481 tagaatatgc gtcaagagat tctggatatg atggtgttgg tgatttaaat cgaataactg
      541 gtatctctag agtagtagat gaaatattta ataataattt tagattagtc ctaatgggag
      601 taggcattgg caatgcagag tttaccaatt tttttgtcag tgagttttat aaatcgtttg
      661 gtaatcttca ttatcagtat tttcatcata tttggttatt tatagaagtt ggaatattag
      721 gagtttcgat attcctattt tttataataa agacattttt caatgcaaaa agatctttta
      781 tgaaggataa agatgcaagt cttgttaaag ttattgcaat tatgtcggta ttcttttttg
      841 tgtataacac aacacttagg ggtgaaacaa cggggccact gatttatatg attttagcca
      901 tgcctgaagt tgtgaaaagg aaagagcatt attatagtga aaaaaataca aaaggagaga
      961 tattatgatt cctaagaaga tacattactg ttggttcggg ggaaatccac ttccgaaaaa
     1021 agatagaaag tgcatagaaa gctggaaaaa gtattgccct gattatgaga ttattgaatg
     1081 gaatgaatca aattatgatt taaataagaa ccgttatatg gccgatgcat ataaagaacg
     1141 taaatggggt tttgttccag attatgcaag atttgatatt atttataatc atggtggatt
     1201 ttatttagat acagatgttg agctaattaa gtcgttagat atacttcgtg aaaatcatgc
     1261 ctatatggga tttgaagggg aaatctgggt taatggcggt attggttttg gtgcagaaga
     1321 aggacattct gttattaaag gtttaaggga tatttacgat tcattaagtt tttatcaaaa
     1381 tgatggtaca ttgaacttga aaccgagtcc gtattatatt acagattatc ttgaatctat
     1441 tggattggtt cgcgataaca gcgaacaaat aataaatggc ctgcatatct atcctacgga
     1501 ttattttgct gccaaggatt atgatactgg aaaaatttat aaaactagta atacaataag
     1561 cattcatcaa tataatgcat cttggaaaac acctaaacaa aaatttgttt atagattaaa
     1621 atcaacaatt ggacaaaaaa aatttaacag gttacttgca ctgaaaaaat ttttacactt
     1681 ataaaggaga aaagtacttt gaagaaaatt tgtttcatta gtggtgtaat ttctcgttct
     1741 gggggaactg aaagagtagg gagtattatt gccaatagct tgtctgattt aggttatgat
     1801 gtcactattt taagcttttg gaatagtgga gagccttatt ttgggttaga taagacgatt
     1861 aaggttgatt atatgttaaa tcctaaaacg gagggaaaat tatatagaac ttatttatat
     1921 ccggtttgga aattgcacaa gtatatatgc aaaaatgaat ttgatatagt aattgatatt
     1981 gatactttgt tgtctttgta tactacaagg gcaattaaag gaacaaagtg caaattgatt
     2041 tcttgggaac attttaatta ctggacaatg gaaatgctga aagaaaagaa gagattcaag
     2101 gctaaaaaat tgataaaaaa atatgcaaag aggcttgtgg ttctcacaga agaagatcgt
     2161 caaaaacata ttgatgaata tagtttatca gaagattttg ttataactat gccaaatccg
     2221 tgtatatcta atatcgagta cgaatatcat tatgataaaa agagatttct tgcagtaggg
     2281 cgattagtgg aagagaaaaa ttttgctgac atgataagtg cttggaaaat tgtagaaaaa
     2341 aaagtagatg attgggatct tatcattgta ggaaaaggtg aattagaaaa agatcttcgc
     2401 cagcagataa aagatttaaa tctgcaaaga gtaactatag cagggcattc agaccatgtg
     2461 gaggaatatt ataaatctgc atcttgttat gtgcttagtt ctgtttatga aggatttcct
     2521 atggttatat tagaagcaca gtcttatgga ttaccagtta tttcttatga ttgtaaaact
     2581 ggtccgagag atttggtaca tcataatttt aatgggatgt tggttgaaga taagaatata
     2641 gaccaattag ctaagagcat gataatgttt actaagaata ctgatttagc aatgagaatg
     2701 tcactgaacg catataataa tgtacaaaaa tttaatttga aagaaattac aaaacaatgg
     2761 gttgctttga ttgaaaatgt atagtttttt gtggttgtcc aaacatgaaa attagcgtaa
     2821 ttaatcaaag agctagaaga aacctattac atttcaagat tttagaaatt atatagtatt
     2881 aggtaacaat aaaaaataga aagtttggag ccatatgata tgataattaa tcgtaaacaa
     2941 aatgctaaaa ggaatataat tactggaaca atttttaagt tatatcagat tatcattccc
     3001 ttttttatgc gtacacttat gatttatcag atgggaatgg agtatgctgg attgaacaac
     3061 ctgttcacat ctatatttca aatgttaaat ttagcggaat tgggaatagg tgctgcacta
     3121 actgtaagta tgtataaacc tatagcagaa gacaatagaa aagagatttg tgctttactg
     3181 aacgcatata aaaagtactt ttcaattatt ggatggatag tgtttacatt aggattgtta
     3241 tgcattcctt ttttgagatt tttagttaaa ggttcgattc ctggaaattt aaatatgaca
     3301 gttttgtatt taatgtattt gataaatact gttttgtcgt attggttatt ttcatataga
     3361 aagagtctaa tatatgcaca tcaaagaaat gatgtaatta acgtgtctat gctaattact
     3421 tttacattcc aatttgtaat gcaggcatat gttctgattt tcttaaaaaa ttactatttg
     3481 tatttattgg cttctatttt gggacaaata gttcttaact taatgtgtgc tgttgtttct
     3541 caaaagatgt atcctgatta tactcctgtt ggtgagttag agaaagaaac tattgacgat
     3601 attataaaaa aagtaaaagg attagttaca aacaaggtag gcggtacaat attacgatct
     3661 tcagattcaa ttattatttc atctttctta ggtctttcag tacttgcgat ttatcagaat
     3721 tattacttta ttatttctgc cataatgagt atatttgcaa taatttatga atcatgtata
     3781 gcaggtatag gtaacagtct tattgtagaa agtcaagaaa aaaattatat agatttttgt
     3841 gatattacaa caattatagg ttggtttctg gcaattagtt gtgcatgctt tattacatta
     3901 tttcagccgt tcatgattat ctgggtggga agtgataatc ttatgagtat ttcatttgtt
     3961 atatttatga tagttcattt tttcttatat gaaattgatc agttaattgg gttatacaaa
     4021 gatgctgctg gaatatggta cgcagacagg tttagaccat taattacagc aatgataaac
     4081 attgtattaa atatcatcct cgttaattgg ttcggattat atggagtttt ggcggcaacc
     4141 attgtttcag tattgttggt tgatttaccc tggttattat ataatgtttt ccaaaatata
     4201 tttaaatttg aatcggcagg taaatatgtt tggaagctca taagtttgtg tgtaatagct
     4261 tttgttgctt cttatttaag ttattttgct tgttcgtatg ttaatatcgg aaatttcaaa
     4321 gtgataagtt tagtgataaa tttagttata tcggttgtca tatcgatttt agttattctt
     4381 ctgtttaata tccgtaataa gaagtttaag tatattttga aagttcttac gtcggtattg
     4441 gtaaaaagat gagaagagac atcagaatga tatataaagt aactacaatt tttaggctgc
     4501 cgaataggcg aacgatgtat agtttttgtt ccaacgaaga ctcttgttga aattggagat
     4561 gatatgaagg agttacaatt ttgactcatg tttatgattg atcggtttta aaagtagtat
     4621 ggagaagctt tgggatcatc ggagaggatc aaaataggta ataatgtttt tataggaatg
     4681 aagtctagaa tactaaaggg tgtacatata ggaaataatg tagttatagg tgcaaatagt
     4741 ttagtaaaca aggacgtatc agacgattgt gttgttgcag gaaatctagc aaaagtaatt
     4801 atgtccctag atgaatatta taaaaagata aacaatatga agaatccgta aaacttgtga
     4861 aactataccg agaataatat gggaaaaata ttgatgaaaa agcattatat gagtttttag
     4921 ggctgtttac agatggagat gacaatttgt cgaagagttg ggagcaatgc aaaatttagt
     4981 aggggattat aaacagactg acaaggtatt taaagaacat gcacaagtat ttcataatat
     5041 tgaagaattt ttgaatactg tgagatagga gttgctacat tgagaaaagt atgtattttt
     5101 ccaatgtaaa agattacgag tttgaaaacg acaccataat attatttttt aagaagatat
     5161 tatagagctt a
//
LOCUS       DAZKCE010000144         5109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000144 DAZKCE010000000
VERSION     DAZKCE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.146"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..473)
                     /locus_tag="ACG8KQ_002657"
     CDS             complement(15..473)
                     /locus_tag="ACG8KQ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9105546.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(486..1817)
                     /locus_tag="ACG8KQ_002658"
     CDS             complement(486..1817)
                     /locus_tag="ACG8KQ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP9105547.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1828..3270)
                     /locus_tag="ACG8KQ_002659"
     CDS             complement(1828..3270)
                     /locus_tag="ACG8KQ_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9105548.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3257..4021)
                     /locus_tag="ACG8KQ_002660"
     CDS             complement(3257..4021)
                     /locus_tag="ACG8KQ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9105549.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4125..5000)
                     /locus_tag="ACG8KQ_002661"
     CDS             complement(4125..5000)
                     /locus_tag="ACG8KQ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9105550.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1543 a         1084 c          750 g         1732 t
ORIGIN      
        1 tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt
       61 tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt
      121 gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg
      181 ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata
      241 tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat
      301 atattatcga ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg
      361 tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata
      421 atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat
      481 cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt
      541 ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt
      601 tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac ttgtgcctcc
      661 atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc cacaccagtt
      721 gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct
      781 tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata
      841 tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg
      901 cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta
      961 gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa gtaatactct
     1021 aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag
     1081 gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc ttttgttatt
     1141 agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta
     1201 taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg
     1261 taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt
     1321 acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc
     1381 aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg
     1441 attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc tactaatcca
     1501 tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt
     1561 gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga
     1621 atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat atccatattt
     1681 ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc
     1741 aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc
     1801 tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat
     1861 aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat
     1921 cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc
     1981 tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc
     2041 ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta actctgtttc
     2101 ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt
     2161 tctcttattg atcaaataat aaaaagtaac gtaatacaaa gcaaaaaaga aaattccgat
     2221 tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca
     2281 tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc
     2341 ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac
     2401 aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac
     2461 catctcttta ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc
     2521 aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat ctccgttgat
     2581 aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc
     2641 gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa
     2701 aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg caaaattatc
     2761 aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga ttgtttgtag
     2821 aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa
     2881 tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc
     2941 aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca aggtagcaat
     3001 cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc
     3061 aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat
     3121 tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg ctaatggcat
     3181 cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat
     3241 ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt
     3301 tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta cgaggataca
     3361 caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga
     3421 tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat
     3481 tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat
     3541 cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt
     3601 ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga tgatgactgt
     3661 ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct
     3721 gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact
     3781 ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac gtcaatgtta
     3841 aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca tgctcgaaac
     3901 ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa
     3961 tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca
     4021 tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataatcat agtccattta
     4081 ctatgtgtca tttggttgcg cctatagata gttacgccct tattttaata gattcttgct
     4141 agatgaattg ccaaatagat gatttcattt tcttgaatgg caagttgaaa ttgctgttcg
     4201 atgatatctt taatctttag tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta
     4261 aaaaaaccaa tattttctac tggcgtttct tcctgataaa tcaagcgttt aaccaggaat
     4321 ttgatatgaa ccattaagcg atgataagtc agtgattctt tatcataaac gtttgaatag
     4381 gatttttcta gatgggacaa aatcgtggtc gtcatttcta ctatttttaa gctctttaaa
     4441 ctattaggct ctcctagctc agcattaatg atatgcatag tgatgaatcc tacttcattt
     4501 gaccctaact cggtttgata tcgatgattg attcgctcga ccacttctac tgctatatca
     4561 tattcatttg gataaaggaa tctcatttct ggcaacataa ttgctgtgac atctctatct
     4621 tctctttttc gttgaatagc aaaataaata tgatctgtca atgtaatata gatatttgaa
     4681 tctaatggag aggagatcct agctgttatc agttcaatca cttcttccgc aaaatccata
     4741 aattcgattg gaatctgcga gactatctca ctgaatcgtt tgatcttttc tttagaatct
     4801 aatacaaata atttgtcagc atcctcaaca tcaaatggct tactcacttt agcagaaaag
     4861 ccaatccctt tccccatcgc aattttttct attccatcgt catcgatcaa tacagcatta
     4921 tgattcagct ttttcatcac atacaccgtt aattcacctc ctctatatga taaaacaaaa
     4981 caacttgcag actccttcat acatattttt gtctccaaac aatttttaaa gacaaaaaac
     5041 gacctaagaa agaacccttt cttaggtcgt gcctaattga atagtagcac cctacataat
     5101 ttacaaaaa
//
LOCUS       DAZKCE010000145         5100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000145 DAZKCE010000000
VERSION     DAZKCE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.147"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..955
                     /gene="purR"
                     /locus_tag="ACG8KQ_002662"
     CDS             137..955
                     /gene="purR"
                     /locus_tag="ACG8KQ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9105551.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1458..2831
                     /gene="glmU"
                     /locus_tag="ACG8KQ_002663"
     CDS             1458..2831
                     /gene="glmU"
                     /locus_tag="ACG8KQ_002663"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9105552.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2925..3896
                     /locus_tag="ACG8KQ_002664"
     CDS             2925..3896
                     /locus_tag="ACG8KQ_002664"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9105553.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3902..4004
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4070..4639
                     /gene="thiT"
                     /locus_tag="ACG8KQ_002665"
     CDS             4070..4639
                     /gene="thiT"
                     /locus_tag="ACG8KQ_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9105554.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4720..>5100)
                     /locus_tag="ACG8KQ_002666"
     CDS             complement(4720..>5100)
                     /locus_tag="ACG8KQ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105555.1"
                     /translation="QLNRIYYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEK
                     ISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIAT
                     LIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1670 a          894 c         1092 g         1444 t
ORIGIN      
        1 aaaaattttt tataggcttc tttttatttt aataaatacc gaactatttt cttatttttt
       61 cataaaatgc tcgaattttt gaagaaaaac ttatataatg taaaacgata aaatttctta
      121 aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca tgacacagta
      181 tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac gttatgaatc
      241 tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta aagaacgagg
      301 gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca tccctgaaat
      361 ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat cagaacaaga
      421 ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac ctgaactgct
      481 gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat
      541 gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc tgaatgcgcc
      601 attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa gtgtcaacta
      661 tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa gtttgaaacg
      721 tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag tcaacggaat
      781 gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat ttgccgaatc
      841 aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg tcaaagatgt
      901 agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag aataagaggt
      961 tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
     1021 ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatctagag
     1081 gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa ttgctcctca
     1141 tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg tttaatgaag
     1201 aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa aacagctttt
     1261 catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca ccggttacca
     1321 aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg ggataaaaga
     1381 aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc agcaaactat
     1441 atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca gggaaaggta
     1501 cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa cctatggtag
     1561 aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg atcgttggac
     1621 acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg ttacaagcag
     1681 aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa ggaaaaaaag
     1741 gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg ctgaacaatc
     1801 tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca caagcagaaa
     1861 acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa aaaatcgtgg
     1921 aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca ggaacttact
     1981 gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac aatgctcaag
     2041 gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat acagttgctg
     2101 cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc gcgttagcta
     2161 aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat ggcgtaagct
     2221 ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca gacacattga
     2281 tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt gtcatcgggt
     2341 cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa aactcagtca
     2401 ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat ttacgaccaa
     2461 aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa aatgctcaaa
     2521 tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg ctgggagaag
     2581 aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac aaacatcata
     2641 cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga cctgtcgagg
     2701 tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt ccagaatatg
     2761 cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa aaactccctt
     2821 atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga ctactattta
     2881 ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca aaacattact
     2941 ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca gagaaaattg
     3001 cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt gatggagaaa
     3061 ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt caatcaacga
     3121 gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca ctgaaacgag
     3181 caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt caagaccgta
     3241 aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt caaaaagctg
     3301 gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt ttctttgata
     3361 ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta gaaaaaggaa
     3421 tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca cgggcacgta
     3481 aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt ccaaaagcaa
     3541 atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt gtactgatcg
     3601 atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg aaagaagctg
     3661 gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct gctttacaaa
     3721 gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat cttccagaag
     3781 atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga gatgcaatca
     3841 aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat aaataataaa
     3901 taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa acccttcgta
     3961 cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac tattttcgaa
     4021 agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta tgggaaaaaa
     4081 tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt cgttgattcc
     4141 gatccagatc ggcagcagtt tttctatttc attagggcag attccattga ctctttttgc
     4201 gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca tcctacattt
     4261 tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt acccgattgc
     4321 ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag gctatgtaga
     4381 cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg gcgcactttc
     4441 cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg cactatgggg
     4501 gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga gtacagctat
     4561 tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt ttgtgccaga
     4621 taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa tcatgtccta
     4681 gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg gcagataaga
     4741 aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga ttaaagtagc
     4801 gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat cttcaatcac
     4861 atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag aaaaaatgat
     4921 cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt tttcaacagg
     4981 tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga tcgaacttaa
     5041 tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc gattcagctg
//
LOCUS       DAZKCE010000146         5061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000146 DAZKCE010000000
VERSION     DAZKCE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.148"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1057)
                     /locus_tag="ACG8KQ_002667"
     CDS             complement(146..1057)
                     /locus_tag="ACG8KQ_002667"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9105556.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1434..2072)
                     /locus_tag="ACG8KQ_002668"
     CDS             complement(1434..2072)
                     /locus_tag="ACG8KQ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105557.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2076..4058)
                     /locus_tag="ACG8KQ_002669"
                     /pseudo
     CDS             complement(2076..4058)
                     /locus_tag="ACG8KQ_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4238..4903
                     /locus_tag="ACG8KQ_002670"
     CDS             4238..4903
                     /locus_tag="ACG8KQ_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105558.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         1628 a          920 c          782 g         1731 t
ORIGIN      
        1 ggctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa gtaataatta
       61 aactactgtt gatttagttc taataaaaag atttagatga acagttttat tgatttaaat
      121 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
      181 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
      241 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
      301 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
      361 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
      421 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
      481 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
      541 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
      601 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
      661 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
      721 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
      781 caaatgaatc acccaattgt ttctttgttt gatcatattg ggaatcgatt tctttggttg
      841 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
      901 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
      961 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
     1021 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
     1081 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
     1141 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
     1201 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
     1261 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
     1321 tttgtttttc ttcttccgct gttgggatat tcaaatcaga cacttttatg ttcgattttc
     1381 cagacatttt tttgttgttt ttattagaaa aagtttgttt tttcatttgt tttctaaagc
     1441 atgatcttct tgtttcctat tttggtcaga tatggatcat gcgtgaccac gataactaac
     1501 gtaccttggg aagcctgttg cgataagatt tcaacgactt gctccttatt tcgtttatct
     1561 aatgatccag ttggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca
     1621 caggcgattc tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg
     1681 tttatattta cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa
     1741 ttcgaaaact tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca
     1801 aaatcttgaa aaagaaaaga gagggcattt ctttttatca agcgcttctc tcttccagtt
     1861 aaatgttttg tttcttgatt aaataaataa aattgccctg aactgaattg atcattcaat
     1921 cctaatatat tcaataaagt acttttccct gctccgctct ctccaactac agtcacaaaa
     1981 tccccagcat ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt
     2041 ttcgcacctt ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac
     2101 tgacttttct tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta
     2161 aagaaaaaaa tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca
     2221 cttgtcgttc cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc
     2281 gaataaccac ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat
     2341 aacatgacat agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag
     2401 taatgaaatg atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat
     2461 gataactgca agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc
     2521 gacaaagagc ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt
     2581 tcccaatacg tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag
     2641 gggatagaag gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt
     2701 tctttttcac tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct
     2761 gtatgtctat atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg
     2821 atcaaccttc cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt
     2881 atccctctgc caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata
     2941 cttccgtttt tattcaaagt ttcatataat tcctgatcgg ctgctgatgc aaaacgattg
     3001 gaataaataa aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc
     3061 tcaccagcga tttcttttgg tgtttcatgg gaaacagcta actgtgaaga agaaaaaacg
     3121 atagttgcta attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta
     3181 aaaagaatgt attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat
     3241 aaaagagaaa tcaaagagat accctatatt ccgctgaaca agaaaacgta catctggaac
     3301 aaataaagaa attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt
     3361 atgaatgtaa taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa
     3421 aagccattat tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac
     3481 aaaaggaata aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta
     3541 cttgaaataa ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa
     3601 tcagtgtgaa atgcttgatt gaatcgttcc acaaatagta aaatgaaccg ttcataggct
     3661 tctttttctc tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct
     3721 tcaatggacg ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg
     3781 acatcccctt gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat
     3841 gtatctactt cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat
     3901 gagacatttt caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt
     3961 ggcaatcgaa tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt
     4021 acctgtttat ttataataac caaggcaaaa ccactcatga taccggtcac tatgattgtg
     4081 agtaaaactg tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg
     4141 ataaaatcat accgtttttc atcaaaaaaa cgtatctatc aaatgtatag gtcatgtaga
     4201 ataaatctat atactgtaga atcgaaagga gaagaagatg aacatcggag aaaaaatacg
     4261 aacttaccgc agaaattgta agcttacgca aaaagaatta ggcgaaaaaa tcggtgtttc
     4321 cgataaaaca atctctagtt gggaaaattc tcgtacaatg ccagaccttg aaatgctgtc
     4381 acttttacat cagactttag gtctgccgat tgattttcct tctattgaat cagctaccga
     4441 atcaatagaa ccaacattgc cggatgaacc tcttgttatc aagaaatctt attcatcaaa
     4501 acaacccttc tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta
     4561 tctttttctt atcaattatc aatataaaaa tgaaacggtt gatcgattca atcctttttt
     4621 gaaaccacaa atcggctata ccacactccc tgtaggatac gtggaaaatt ccatcgaaca
     4681 ttgggtatgt aatgatatct ttgttcatgg tacatggctg tcttttcacg ctggagaggt
     4741 agaaaaaggt aagaatcatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg
     4801 cctgctcact tgggaagcca ttccctcaga ttaccaagaa atcctcatcc aagactatga
     4861 cgatgcctta aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag
     4921 agatagaaaa aatcaatgca catgatctta tcaaaaaaaa acaggggtac aaattaattg
     4981 aaaaagcttt gagatgaaag ttcctaaata aaaaagttca gaatgatagg taaaaaaatg
     5041 actaactatt ctagtattag g
//
LOCUS       DAZKCE010000147         5016 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000147 DAZKCE010000000
VERSION     DAZKCE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.149"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1872)
                     /locus_tag="ACG8KQ_002671"
     CDS             complement(106..1872)
                     /locus_tag="ACG8KQ_002671"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105559.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1832..3586)
                     /locus_tag="ACG8KQ_002672"
     CDS             complement(1832..3586)
                     /locus_tag="ACG8KQ_002672"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105560.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3696..4265)
                     /locus_tag="ACG8KQ_002673"
     CDS             complement(3696..4265)
                     /locus_tag="ACG8KQ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP9105561.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4283..>5016)
                     /locus_tag="ACG8KQ_002674"
     CDS             complement(4283..>5016)
                     /locus_tag="ACG8KQ_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9105562.1"
                     /translation="TENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDI
                     NFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQ
                     FFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLS
                     KTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNG
                     GIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1666 a          945 c          678 g         1727 t
ORIGIN      
        1 tctttcgtgt attcaaaatt agatgctgtt taataaaatt attctttttg aaaaagaatt
       61 ataactatat cccaaaaatt gtaggttaat ttaaagccta tttcatcatc gtgattgagc
      121 ttctttttca tagacgaatt tctcagatag tttagttttc tttatgagat ttttgtatat
      181 ttctgacctt cgatacaact cattatgagt accttgatca actagttttc cttgatccat
      241 cactacaatt ttatttacct tttcaataga cttcaagcga tgcgatataa ttagtactgt
      301 cttatcttgt attaaacgat ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa
      361 actggcagta atttcatcta aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat
      421 ggataggcgt tgtcgctctc ctccagaaag tgaacttccg ttttctccga ccaatgtatg
      481 ataaccgtca ggtagttttt caataaagtc ttcgcagtta gctaaacgag ctgctttttt
      541 cacttcctca tctgacgctt gactatttcc aatacgtata ttttccaaaa tacttgtatt
      601 aaacaaagtt acctcttgaa aaacaatagc gattttgcta aacaaagaag ctggagatac
      661 acgtttaata tcatacccat caattaaaat acagccttca tcataatcat acagtctaga
      721 cactaatttc aagatactag ttttcccgga accactagca cctactaacg cagtcacttc
      781 tccttgttta gccgtaaaag agatatgatc taaaattggg gtattgttat catatgagaa
      841 agagacatct cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc cctcttggat
      901 actggtttcc ttcatagctc taattcgctg tattttagga gctaaataaa atatttcaag
      961 cactgcttct ttcatagaat cgaaggaatc ctttattttt attgcggcta gtaagtaacc
     1021 aacgacgtag agtatagtca cttcacccgt taacagtaag tgaacaccta ctagaatgac
     1081 tactgctaaa ctaacatatg agaaaataga ggataaggcc atgactgaaa aagtacttaa
     1141 ttctaccttt aaatgaatcc tttcactctc ttccattttt ttgaaaagtc tgtcttgaac
     1201 ttttttagat agattaaagc tattaatctc ttgctgcaat tctatagttt cttgaaattc
     1261 ttccgagttt tctctcaaag tatcgtaata tttagtattg gctttcgttt gggatttctt
     1321 tgataacaag attaagacaa aactaaataa cgttggcaaa ataacagcta atcccatttt
     1381 gacattacca accaaaagca tcactgaaat aaaagggaaa aacagagcca taccgccggc
     1441 tttaggtatt gcatgactca tcgcatgctc aataccttca acgtcagaca tgatagtttg
     1501 agataaatct gataaattat gttttgaaaa ataggataat ggtaaatttg acaaatcgtc
     1561 tgcaatttgc actcttaaat gcgcactttc tttataagta ctattatata agttttcata
     1621 ctccctatct aacaaccaat acatgaaaag caaagtaaca aacgatacgg caaaataaag
     1681 ccaacggggc tttgtatttt ctaaaactaa ttcgtctatc agcatcatta taatgaatgc
     1741 tggtaccata ttaatgcagt atacaaaaaa actagaaata gaagctttag ttaaagcttc
     1801 tgcgccctga tcacttaaag caaatttgtt tttataaaaa cttttcattg tcaagcctcc
     1861 aatcatttgc attctcataa ttagtgacca attgtttata cacttgatgt ttagccatta
     1921 attcattatg atttccacgt tctactattt tcccttctga caagacaaga atttcatcta
     1981 cagatttaat gctggataat cgatgagcta tcataataac tgttttttct ttcatcagat
     2041 gttgaaaagc cttttgtagt tcatactcat tatctggatc aatagatgca ctggcttcat
     2101 ccataataac aatctttgca tcttttaaaa ttgctctggc aatagctata cgttgttttt
     2161 ctcctcctga cagataaact cctttactac caattaaggt atgctctctt tcgggaaatt
     2221 tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta cttgctggcg
     2281 cttgaggatt tgctatagaa acgttatcgt aaatactctg attgaatagt ttcgtatctt
     2341 gaaagacaaa agcaattgtg ttaataattg tgtcttttga atagtgattt aaaggcttac
     2401 caccaatttt aatctgtccc ttatccacat tgtagaaacc tgaaattagt ttagctatcg
     2461 tactctttcc gcttcctgat tttcctacta atgcataaat tcttttttgt tctaaaacta
     2521 agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa
     2581 tgtcacaatt tttaatctgt gtctcgttcc catattttag ttggtcttgc ttcatttcta
     2641 aataaaggga gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc atacttacat
     2701 acattattct catgaaagaa acaaaaataa ttccacttaa aaaaaacaac atcaacaagt
     2761 caacagctaa tatcttggga gtggtaagag aagttataaa aaaggttata ggaactatca
     2821 aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt ttcccactca
     2881 aagaatactt atatgcaaac ttagcataat cgttaattgc tttattcaag gctttaaatg
     2941 atgttacttt agcaccaaat atctttataa cttgaattcc gcggatatat tctaccgttt
     3001 cagagcttaa cacatctaac gattcttgat atttttgaat aaaatttgta ttctctgtca
     3061 ttttctttaa caagaataca ccaattacta acatggcgat tagaaccaat ccaattttaa
     3121 agttaatgaa aaagcctaag gcaactgata aaacaggtgt tactatggct tgagcactat
     3181 ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc gttgtcatca attgtctttc
     3241 gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa
     3301 ttcccttttt tctcaaatta gtctctaagc gaaacccaag gatatgagaa aataagccag
     3361 aaaaaatata aaataaagct cctaaagtta accaagcaac agtttgaacc gcataactct
     3421 tagctaaata ttcatctcct acaataatta gagactgaag aaatttgtaa atactgtaat
     3481 atccatagac tgttaatacg acagaaaaat cagaaaataa agtggctaac catccgtagt
     3541 atttcatttt agggacataa ctaaataatt ttttgtatac tttcattttt tcctcccccg
     3601 cattcaattt cttctcttat tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt
     3661 agagatgtac aaacagataa aaggtaaact acgttctatt ttttgtattt ttttaagact
     3721 tttcttccaa tcaatacacc aatcatagat aaacttgcta caactaacac tgaaatgatt
     3781 ccccatatac ctaccgcata gtggatcatt gcatccattg ttgcagcaga ttgtccttta
     3841 tctatccaat cttgacgaaa acgatctgta aagaaccata taggaatcca ctgtccggca
     3901 gcactgccta attgcattag agcccaagac gcaacttgag gaattgctcg atcatatcca
     3961 aatacagata gaataatatc agctaagatc gccatactaa tcgaactgat aaaccaaggt
     4021 aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa
     4081 attccttttc tagggtagtt atagctaata taaacaaata tgattccaga taataatcca
     4141 aaaatcccgg gggatatgct gtgaccaaac gccccaccaa tactactaac cattattgaa
     4201 aaaaagtaaa ttactaggga aataactgta gctaaaccaa taaaaatata atcttttgtt
     4261 ttcatatata ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt tctatcaaag
     4321 ttgaatcttc tactctaagt tttatatctt tttttattcc accatttttt aaataaataa
     4381 ttctattgca tgttttataa agaaattccg gatcatgagt agctataact actgcaccct
     4441 tttcttttaa gtctttaata aggtcagcaa tcctttccat attaatataa tccaacccac
     4501 ttgtaggttc atcaaataga aataaattag tttttgacaa tgaagctata gaaaccaata
     4561 atctttgttt ttctccaccg gataaatcaa acggtaattg atctttcatt ttataaagtt
     4621 ccattttttt taaaattttt tcttgctgat ctttactaac tatttcattc cccagggcta
     4681 actcgctcct cactgattct gtgaaaaatt ggtaatccat ttcttgcatt actaaaaacg
     4741 gcgatgacaa atttttttta ttttcgatta tctttccttt ggtagggcga ataatcttca
     4801 tgagtattct tagtaaggtt gttttcccca caccgttttt ccctaccaaa ccaataacat
     4861 ctccaacttt gacatcaaaa tttatgtctt ttagaatatt ttcataagta atttcttcaa
     4921 cttttaatac atctgcactt accttttcga tatagggaac ttcaatcgaa ctattaaaaa
     4981 tgtcaaattt ccttaatgcg ttattgtttt cagttt
//
LOCUS       DAZKCE010000148         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000148 DAZKCE010000000
VERSION     DAZKCE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.150"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8KQ_002675"
     CDS             85..972
                     /locus_tag="ACG8KQ_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9105563.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8KQ_002676"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8KQ_002676"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9105564.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8KQ_002677"
     CDS             2274..2720
                     /locus_tag="ACG8KQ_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9105565.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8KQ_002678"
     CDS             2993..4345
                     /locus_tag="ACG8KQ_002678"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9105566.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8KQ_002679"
     CDS             4516..4803
                     /locus_tag="ACG8KQ_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9105567.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKCE010000149         4861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000149 DAZKCE010000000
VERSION     DAZKCE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.151"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8KQ_002680"
     CDS             <1..390
                     /locus_tag="ACG8KQ_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9105568.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            709..1437
                     /locus_tag="ACG8KQ_002681"
     CDS             709..1437
                     /locus_tag="ACG8KQ_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9105569.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1582..3609
                     /locus_tag="ACG8KQ_002682"
     CDS             1582..3609
                     /locus_tag="ACG8KQ_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9105570.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3717..4814
                     /locus_tag="ACG8KQ_002683"
     CDS             3717..4814
                     /locus_tag="ACG8KQ_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9105571.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1719 a          794 c          986 g         1362 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaata ttttacactc tcgtcaagtc acattttgat agataggagg gggacttacg
      481 aattatttta aaggctatga cagctgtatt tctagattgt gtttgcatca agttgcaata
      541 aaaactagaa atcaaatcgg gctgataatc atttataact aatcgataca taggaccaaa
      601 tataggtcta tgttctttta tttcataagt atcgtaaaac agaattttgc tatggattaa
      661 tccaatcgac tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa
      721 atcgtattaa cagcagcaat gatgttattc agcatggtag caagtacgac ttttgtttct
      781 gcaactgaag tatacccaaa ggaatataac acagaaggaa cgattacatt tgaagcagga
      841 gatgagggtg taactcctcc tgttgatcca gagaatcctg atcccaataa acctgtcgat
      901 ccatcagatc ctccaagccc tggaacagga ggggcattat cgattgatta tggatcaaaa
      961 ttcaaatttg ggactcaaaa aatttctact gcggataaaa cgtattatgc agctgctgat
     1021 gtgatgaacg atgggtctag aaaaccgaca tatgtccaag tgacagatag gagaagtacc
     1081 ttatctgggt ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc cagtggggat
     1141 gaactagttg gagcacagct gaaatttacg aaagggcagg ctgtttcttt ggttgatcca
     1201 acatatacgc cgcaaacggt gaattctgaa ttaacgttga caccgggagg aaacaatacg
     1261 ttagcaatta atgctaaatc aggaacaggt gtggggactt gggtctatcg atttggtgca
     1321 aatgaaaatg aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta
     1381 gcgcaacaat attccactaa attagtttgg acattagaag atacacctaa caactagttt
     1441 actgaaaaga actgttaaaa ataaatacgt agtcatattg tcttgcgagg taatcgagca
     1501 aaaattgtca aaggctaggc taaatttgtt tggatacgaa tagttcatct aggaatttaa
     1561 cgatcaggag gaaaagatat attgaaaaag aggaggatcg tgctcatttt aagcagtctc
     1621 ttagtgacta atatctttcc gatcataggg tactctttgg aattgaatca gttggagcat
     1681 ggaaatagaa gagacgaaga aagcaaactc acttcgacat tcgacagttt aatagaatcc
     1741 aaccagacac aagaaagcag tgaggattta agtatttcga gtagtggaga gaccactgac
     1801 aacgtagaag ctgagggttc ggacaaacaa ggactagaag atgcaccaat ttctgcggtt
     1861 gagctgcctg acgatcccaa tattatttct atcgataaaa tttttcaaac acctatcggt
     1921 actagcacgt ccatattgga aaatgggaag ttactgcagt tgaacccagc agaaaggtct
     1981 caaagaggag ctatttggtc aaataagaag attagtttac tctcggattt tacatttaaa
     2041 tcttatttat acttaggaaa tcaacgagat agagcgggag atggcatgac ctttacattg
     2101 actggcgatc ctagaatggc tagtaatccc agtcaagtta ttggatcgcc aggtatggga
     2161 attggctcat attctactcg ttctggccaa ccttacattg cgaatgcgct ctcgattgag
     2221 tttgatacgt ataaaaatag tggttcttct gatcgaatgg atcgagagat aactgctgat
     2281 aatagagggc atggacatgt cgcttttgtc acgcctaaac caaataataa taattattct
     2341 ggagagcata ctggcgtgac ggtagctcct acatttcttt ctaatggcac atggcgcttg
     2401 ttaataatta aatgggacgc ggagcaaaga agtcttactt atgatttgga aggtgtcggt
     2461 cgctcttctc ggattatacc tgatctcatt aggcagtttg gttctacaga agtaaattgg
     2521 gggtttactt cttcaacagg tacttttttt caagaaaatg ctgtcgctgt cacgcgactt
     2581 ccatcgaatg tcacatcaac agctgatgta aaagtcaacg atagtgatta tggggccatc
     2641 gctgaagctg gaggaaacga taagataact atcagaaata cattgaaggt cgagggggat
     2701 tttcttgaat ataagaaaat gcagactagt atcgacctcc cacaagaatt aactattccc
     2761 gagcatatct tgatcaatgg aaatcaaatt gatgccaaag atatcttgat aaccggaaat
     2821 cgagtcacgc taaatctgac agattatttt attgctggag atacgttaat aatcgaattg
     2881 gagacaactt tgcaggaagt aattcctgaa aaagtactca ctatgcattt tgaatacctt
     2941 gaaaacggga atttgattga gcaatcgaat gaaataaacc taaagatcgc aaagctgaag
     3001 gagatagata tccatgttta ttataaagat aaagacagtc agattgatat tgctgaaatg
     3061 aaaacaatca ctgggaaaat aggagagaag tataaagaaa ctcctattga tattgaaggg
     3121 tatgtttttg actcggattc agcgaataca gaaggcatct tctccgaaaa tacagaggat
     3181 atctattttt attacagaaa aggcaaactt tatctctcaa ctgctcctca atatgtcaat
     3241 tttggaaaac ataaaatatc cagtagacca ttaagtgaat tcggacagtt ttcaggtggg
     3301 ttgaaaatta tagatgaacg ggcttttgga agttggaaat tacaattgaa acaagagaaa
     3361 gttcttacaa atggagattt tgagcttcca gaagctcttt cctttgttac ctctgaaaat
     3421 acaacggtta tagggacatc cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc
     3481 gatttgtcct ctcttttaga ttctacagct catcgtggaa tcagaataaa tattcctgta
     3541 gaaaaccaaa gaatcggaac ctttgagggg aaactatctt ggatacttgc agatgtccca
     3601 ggaaattaac aggaagagaa gaatataaca aaattattga gtttcaagaa gggcacaggt
     3661 accataggct cttgtgtccc acagagacaa gattttgtta tagtcttctc ttacatatga
     3721 ttaaaggagc gattttcttg agaccaaaac aacaattatt tataacactc ctagttattt
     3781 taggggcact ttttaacaat atatcagtgt cagctagtgc atttaatttt tcagtgacgc
     3841 cgataacttc agagaatcaa atcgacaaga gaaaaactta cttcgatctt caacttgatc
     3901 cggatcagga agtagaagta aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa
     3961 tcgatatttc tgtgaatagc gcaaccacca attcaaatgt gatggttgaa tacggaaaaa
     4021 acgagattga aaaagacatg agcctgatct ttgatctggt agattatgtg agctatccac
     4081 agatagtgac gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga
     4141 atgagcgatt tgatggtgta ttggctggtg ggatcacatt ccaagaaatc cagacggaaa
     4201 aggaccaaac agaaactaag gatcaaagct tatcgattga gaatgaatac acctatatta
     4261 ttgctttgtt gatgcaacag acactgaacg aagttgctcc gaaccttact cttcatgaag
     4321 ttaaaccaga tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa
     4381 aaacctatat caatcaagta gttattgaaa caaaaattac aaaaaaagga cattctgaag
     4441 ttttatatca agaggaaaaa gaaggattgc agattgcgcc gaatacaaat ttttcttttc
     4501 caactgcttt aaatggtcaa ccgctaaccc caggtgaata tcaccttaca atgactgttt
     4561 taggaaatga aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact acgatcaact
     4621 ataccaatca atgggtattt gaaaaagatt ttacaataga cggaaaagta gccaaggaat
     4681 tgaacacgac ggatgttacg attaaaaaag acaataattg gatttatatc ttagtagctt
     4741 cacttttctt attgtttatt ttattatgca tacttttgtt tgttttgaga aaaaaagaag
     4801 agaaagaaaa gtaaggttaa gataacaaat agtaaatttt tattattgta ggatggttgg
     4861 g
//
LOCUS       DAZKCE010000150         4814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000150 DAZKCE010000000
VERSION     DAZKCE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.152"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..1100)
                     /locus_tag="ACG8KQ_002684"
     CDS             complement(138..1100)
                     /locus_tag="ACG8KQ_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9105572.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1102..2541)
                     /locus_tag="ACG8KQ_002685"
     CDS             complement(1102..2541)
                     /locus_tag="ACG8KQ_002685"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9105573.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2726..4672
                     /locus_tag="ACG8KQ_002686"
     CDS             2726..4672
                     /locus_tag="ACG8KQ_002686"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9105574.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1540 a          816 c          784 g         1674 t
ORIGIN      
        1 gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacaagttga caactccttt
       61 ttgtttacaa atcaattgga tatctacaaa ttgtattttc taacgaattt tatagacatt
      121 aaaaaattaa tttaacgtta aatacttcct ccagaaagta ttgagattgg taaaatataa
      181 tccttatttg tcttttcgcc tttaattttt ttcagaagaa tatcaacaat aagacttgca
      241 atttcatcaa ttggttgtct tattgttgtc aattgtggga aaaatttctc aataaaagag
      301 gtaccatcgt atccaataat ttttaaatct tctgggatat taagtttaag ttgatgagca
      361 attttcatag tgagaatggc tgttaaatca tcagaaacaa aaataccatc tggtttgtga
      421 tttataatag ttgactttat ttccatttca cgacggatag tagacagatt attaggaatt
      481 ttgaaaattt ttccatttgg attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga
      541 gaatccgtgt tatcatttcc tgtaatcata atggtatttt gacaaccatt tttttgtaaa
      601 gtcttagctg caagttttcc cccttcaaag ttatctgaag aaacaatgga aacattagga
      661 gctaaatttc tatcaaaagc aacaataggt gctctcacac gctcataatc agcaattcca
      721 agattatggc ttgatgagat tatgccatca acttggttgg cttcaagcat ttcaatgtat
      781 tctctctctt tagcaggatc attttgacta ttgcaaataa tcgttttata tccatgttta
      841 aataactcta tttcgagata ctcaatgagt tcagagtaaa agatattact aatattagga
      901 aaaataagac caataagctg aggtgacttt ccttgaagtc cacgtgctaa attatttggt
      961 ttataaccaa gagttttcat agcttcatga actttttgtt ttgttttttc tgataaatat
     1021 cccttattat taatgacgcg tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca
     1081 tcagttaatt ttgctaccat tttaatattt taattcaaag tattttccag taggttcgcc
     1141 tgatttgatt tgaataccat tttggtgatt ttcagggaat acacgactag taaacacttt
     1201 ttctccttta ttaataaaaa tctcaatgat tgagttatct acaaaaatgt tagcagttgt
     1261 ttctttagta tcaattgagc attcacgaat cgtaccaaag tcagtggcat attgaatgcc
     1321 tgctttgctg cgatctaaaa taatttttcc ttctttggta tctacaatca agctaagtcc
     1381 atttccttta ctatcagaga aaagaagtaa ctccgatttc tgatttggag gaaaagttag
     1441 ttcaagctca taagtattat ttgtttctgc ttttgctgta aattgttcag aactagcacg
     1501 taatgatgta atagcttcta ctggatattg gtaaagttta ccatctttta ttgaaagttc
     1561 ttttacaaga ctcattgctc cctgataatc atacttatca gttggataat cgatatcagg
     1621 tagtccaatc caactgacaa tcagagcacg accatcagga gtattgaatc cttgagtcgc
     1681 atacgcttca aatccataat caagattttg aatttctgaa gcgcccacaa gttttacttt
     1741 ttctatgtca agcgattgac agatcttaaa agtattcgga tagatattcc cataatttag
     1801 ctcagattta tccaaccctt gaggacaata aatgagaaca ggttttttgt ctacaaatac
     1861 tagatttgga cactcaatca tgtattctga accagtgccg ccaaaatcaa ggttgccaac
     1921 ttcctcccat gtttccacgt tattgtcatc cgctttgtaa agtttgataa agcctgattt
     1981 ttctaaattt tgtgctccaa caatcgcata caattgtcct tcataattaa acatttgtgg
     2041 atcacgaaaa tgatcagtta catcagcagg tttatgaata agaacattct cacctttcgt
     2101 aatgttgtag tctttatcca tccatgctcc gatttgaagt gtatcacgtt cccaattttc
     2161 atttcgaacg tttcctgtat agagtaagaa tagattcccc tctatttcat atgcagaacc
     2221 ggaataagcg ccgtggctat ctcgttttgt gtctggaaga agttctgctc ctgttacatg
     2281 aaaatgaact aaatcatctg attcattgtg tacccattct ttcaacccat gagcagctcc
     2341 aaatggccaa ttttgataaa aaagcgtata ttttccgtta aaaaaagaaa aaccattagg
     2401 atcgtttaac aatcctgttt taggttcgat atgatagcta gcacgccaag gtgatcgttt
     2461 tacattatcc ttaattttat ccatctcatc tttagtccaa tcagaataag cacgatatcg
     2521 tatttctcga ggtaagttca tattttactc cttcaattta tctttttaca actagtatgg
     2581 taaacgtttt ccgttgaaaa atcaatcatt tcatttaaaa aaataaacta tatgacaaac
     2641 gtttgacata tttttaagat atgctaaaat aaatttgcat ttcagtgaac cgttttcacg
     2701 taaaaataaa aaaggagtga ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca
     2761 agcggtcgga aaagataacc ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt
     2821 actaaaagat gacacgaaag tgaatcaaaa agctttagac gaaaatccag atgtgaaagg
     2881 cacatttaaa attgatggac aatatcaagt gattatcggt gcaggtgatg ttaatttcgt
     2941 ttatgacgaa cttatcaaaa aaactggact ttcggaaatt tcaacagatg atttaaaaca
     3001 aattgctagt aaagataaaa aattcaaccc cattatggca ttaattaaat tattatcaga
     3061 tatttttgtt ccaattatac ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa
     3121 ctttttaaca gctgctggac tttttggacc aaaatcaatt gaagaaatgt atcctgcgat
     3181 tgaaggtatt tcctcaatga tccaactgat gtccgctgca ccattcatgt tcttaccaat
     3241 tttagtaggt atttcagctg ctaaacgttt tggagcaaac caatttcttg gtgctgcaat
     3301 tggtatgatc atgacaacac cagatcttgg tggctcaact gaatattgga atatttttgg
     3361 ttatcatgtt tcacaaacaa actatgctta tcaagttatt ccagttttag cagctgtttg
     3421 gatattatct atattagaaa aattctttca taaaaaattg ccatcatcta ttgactttac
     3481 attcacgcca ttattatcag taatgattac tggatttttg actttcacag ttattggccc
     3541 tgtaatgtta ttgttgtcta atggaattac agacattatt gtttggctat ataacactac
     3601 tggatttatt ggaatgggta tttttggagg aacatattca cttatagtca tgactggttt
     3661 acatcaatca tttccagcta ttgaaactca attactttca gcgtggacaa atggtatcgg
     3721 acatggagac tttatctttg tagttgcatc tatggcaaac gttgctcaag gtgctgcaac
     3781 atttgccatt tggttcttaa ctaaaaattc taagacaaaa agtcttgctt cctctgcggg
     3841 tgtctcagca cttcttggaa ttacagagcc tgccttgttc ggagttaact taaaataccg
     3901 ctttccattc ttttgtgctt taattggttc aggaattgct acagcagtag caggattact
     3961 taaagtagtt gctgtttcac ttggttcagc aggtttcttg ggatttcttt caattaatgc
     4021 aacatctatt ccattttacg tattgtgtga attgattagc tttgtagttg cttttgcaat
     4081 tacttacttc tacggtaaaa caaaagctgc tggtatcttt aaagcagaag aaattgctga
     4141 acaaacacca gttaattctt caaaagtagc aacaacagat ccaatatcta ctgatgttga
     4201 atcaacacaa acaaaagttt catcagaaac aattgttagt cccatttctg gtaaagtgat
     4261 tagtctttct tcagtaaatg atccagtctt ttcatcagaa gctatgggaa aagggattgc
     4321 cattaaacca aatggaaaca cggtccattc tccagtagat ggtactattc aactcacgtt
     4381 tgaaactgga catgcttatg gtattaaatc aaataatggt gctgaagttc taattcatat
     4441 tggtattgat actgtatcaa tgggaggaaa aggatttaca caaaaagtta ctactaatca
     4501 aaaagttaga aaaggcgata ttcttggagc atttgaccgt tcaatgattg agaaagaagg
     4561 tcttgatgat acaacaatga ttattgtgac caatacttcg gattattcag aagtaaatat
     4621 agtagctgaa aatacagtca ctgagggtac tgatttactt gaaatcaaat aaatatattt
     4681 ccctgagtaa agatggaatt ttctttccaa aatcaaataa tatagattaa tcaaatattt
     4741 gagaataaca ttttatttaa tcttttcata tatcaattgt atgaaaaatt attagaattt
     4801 taaaaaatga taaa
//
LOCUS       DAZKCE010000151         4723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000151 DAZKCE010000000
VERSION     DAZKCE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.154"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8KQ_002687"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8KQ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9105575.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8KQ_002688"
     CDS             724..1878
                     /locus_tag="ACG8KQ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9105576.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8KQ_002689"
     CDS             2093..3061
                     /locus_tag="ACG8KQ_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9105577.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8KQ_002690"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8KQ_002690"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9105578.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8KQ_002691"
     CDS             4091..4699
                     /locus_tag="ACG8KQ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9105579.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaa
//
LOCUS       DAZKCE010000152         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000152 DAZKCE010000000
VERSION     DAZKCE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.155"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8KQ_002692"
     CDS             690..1658
                     /locus_tag="ACG8KQ_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9105580.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG8KQ_002693"
     CDS             1726..2484
                     /locus_tag="ACG8KQ_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP9105581.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8KQ_002694"
     CDS             2552..3262
                     /locus_tag="ACG8KQ_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9105582.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8KQ_002695"
     CDS             3298..>4425
                     /locus_tag="ACG8KQ_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9105583.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKCE010000153         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000153 DAZKCE010000000
VERSION     DAZKCE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.156"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8KQ_002696"
     CDS             73..234
                     /locus_tag="ACG8KQ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9105584.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8KQ_002697"
     CDS             253..1530
                     /locus_tag="ACG8KQ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9105585.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8KQ_002698"
     CDS             1540..2196
                     /locus_tag="ACG8KQ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105586.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8KQ_002699"
     CDS             2196..3572
                     /locus_tag="ACG8KQ_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9105587.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG8KQ_002700"
     CDS             3702..4115
                     /locus_tag="ACG8KQ_002700"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9105588.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG8KQ_002701"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG8KQ_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKCE010000154         4373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000154 DAZKCE010000000
VERSION     DAZKCE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.157"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..292)
                     /locus_tag="ACG8KQ_002702"
     CDS             complement(62..292)
                     /locus_tag="ACG8KQ_002702"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9105589.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(386..724)
                     /locus_tag="ACG8KQ_002703"
     CDS             complement(386..724)
                     /locus_tag="ACG8KQ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105590.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1652..1768)
                     /locus_tag="ACG8KQ_002704"
     CDS             complement(1652..1768)
                     /locus_tag="ACG8KQ_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9105591.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2244..3122)
                     /locus_tag="ACG8KQ_002705"
     CDS             complement(2244..3122)
                     /locus_tag="ACG8KQ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9105592.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(3445..>4373)
                     /locus_tag="ACG8KQ_002706"
     CDS             complement(3445..>4373)
                     /locus_tag="ACG8KQ_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9105593.1"
                     /translation="INHDFLISSFSSLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAF
                     VLGILSTYVLWLEPFAVALVAFSSRFVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQL
                     PIVSLYFFMGVVFALIAAVITKLLDSRPEQTIEKATLKERFHEEPLVIIDSVFYSAAL
                     FLSVYVSHGLNLHNPYWLTLSCASILLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFV
                     PFTQLQTIFLITFLYGIAQFLVARNYAVANIFLNPMALMLSTLIRGAYLISLIEYRFL
                     GIVIGSFIGLGVAWVMTVGLQHYLTVVRQKLE"
BASE COUNT         1446 a          823 c          666 g         1438 t
ORIGIN      
        1 caaagatatc gaaaaaacaa taacataata tgtgtcaaaa tttttcataa taagaactcc
       61 cttactgttg catactaaca taatccaaaa actcttctac ttcagctaaa gacattccac
      121 tttctttagt ccaaaaaaca gttttttcta tcgatccttc tctgtaaact gtaaatgtcg
      181 gagtcgagtt aattgagtag cgactcttga attctttcca ctcattttca tcttctctaa
      241 cttttgctac attcacgtaa taaatatttt cttttccctt ttctaccagc atatcgaata
      301 gcttcctgct tttgatgatc gatttcttgt gctacatcta gtaaattttc atcaatttct
      361 atatcataaa gtatttcact agtgtctaca tccacaatat atacttttta tcagtagcaa
      421 tttttgagta atccgttaaa atgatctcag tctcgtcact ttcatcagta taaatatacg
      481 ttaacaatga actcaccttt tcaatcggtg ttagctctaa gcctactgta ttttttacta
      541 ttgagtcatt atttaccacc ataacttcgt actgtatata ccatcctatt tggtttccat
      601 caatatcagt aattggctca ctagctaatg gagtctccgg aacaatattt tcctgatatt
      661 tcttaaactc ttctgtagca cgaaaaaggt tatcatgttc ctcacttgaa actggttgag
      721 tcatagaaaa tgttacttgg ctaaacagag ggaaaataaa cactacaatc aacaaagtat
      781 aaaaaaaagc ccttcttttt cattttttat cttccaatca caaattgaaa aacttattcc
      841 tgcaactttg agtctagtat cacaatcgac tgactacaac tattcattta cattctactt
      901 taagttcctc cttaaccatt aattttatta aagatacttg taatagtgat tctcacaaaa
      961 tttcatgaaa aatactttat tactgtatct aaaattccaa tacacaactt atcgactatt
     1021 atctgaaaac caaaacttat tattccttga tgttattcta gcaatagtaa ctgctttttt
     1081 atttttttgc atattttttc ggcgttactt tttaaagtta actttttcga caaatagaaa
     1141 gagatacgaa tcactgattt ttttgaagaa aagaaatgga gtgagctgaa cgttagagat
     1201 attgggagct ttcgtaactg cttattaaac gataatggtt atttgcaacc gtattgctcg
     1261 cttctacatg aaatatttca tgaaatattt cgcaaaacct ttgattatgt agttacgttt
     1321 ccattcttga ctaaaaatgt ttcaaaaacg aacaaaggtg ttaaaaggta aagtgatcaa
     1381 tactactggt caaaataaga ggttgaaagg gaattgaaaa ataactacag ataatacttt
     1441 tataaacgta cgtgctttgt ggttctgtag ttaactatat aactggaatt agggaatggt
     1501 atcaatttga aaagaaagat tctaccaatt tatcatatgt taaaaatgga aaatcaacag
     1561 cagttctttc ggaaacctta cacaaaaacc gattattgaa aatagccgaa aaacgttttt
     1621 cggctatttt tttaattaag tttccttctt cttacctttt cccactattc cgtttcacat
     1681 ctttgatgat cttcagcgca cgttttcgtg ttttgatatt tgggcttttc aagcggcggt
     1741 atgcacttga gatatctgtt ttttccatat ggacgaccca ctttcttctt tgattgtagt
     1801 catgctaaaa aacacaaatc gaaacaatta cgatttgtgt tttaaatggt atcaaaagtt
     1861 aaaatgaaag tcaacttgtt aaaacagtcg atcagactag ctgattgact cctatctctg
     1921 gaaataaatt ttcattatta cgttttagct acctaaaaaa ggccctcttg tccgttaact
     1981 ttaatatcta ttgttatagc tagtttacct attgaacgta ctgtgtgttc gatagaacac
     2041 taaaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa ttcacgaaaa
     2101 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc
     2161 cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtct cacgctcttt
     2221 taaactattt cgaagtacta aacttatgaa gtaagaatac ctaagccaga gccaatgtgc
     2281 tcgcctgcaa gcaattgctc ttttgcttgt cgctgtaaac tcgtactttc tggataatca
     2341 tgtatttggg catctactcg tccaaagatc caagggactc cactatcgtc ctgttcatca
     2401 actgataata atcgataatc ggctgcaaaa agacagattt ttgactcttt tttcaaccat
     2461 ttagtaagat tgggtgatag taaccaagta tctgtgtaca tcacttggta atggtaatca
     2521 ggaaaataaa gttcaaaaaa acgaatggca ctttcgtaac tatctcttat cttattctga
     2581 gataaatctg catcggaagg aatatgaaga gaaaggcact gctttccttc taatcttaga
     2641 tccgtgtacc ctttcggaaa atatgtcatt tcgtactcca attcaccaat ccgaaagatc
     2701 aagccactag tgtaatgcca aatccaaaaa ccacggtcaa agcgatagct tcctgttact
     2761 tggaaatgtt catgaataaa tctagtaaaa gcattcattg ttgccaaata gatagttctt
     2821 ggaatcttca atggttgata aatttttttc caactttgag aagctgcata tagataaata
     2881 gctaatgctg caaaaggttc ctcttcatag actttgtcta ttttctttgc agtcgccttt
     2941 gctttattag gatccattag ttcatgacta tattgctcaa acacctcatc aaggatgttt
     3001 tttgcttgta caatcatttg ctctataacg actttgggaa gagttaaagt ttctgccaga
     3061 atttggattt cattgcttat tttcttcatc aaatttcccc ttttcatatc tttgaacctc
     3121 attgtttaaa tgactgatta tccggacacc atatggtata aacatacttt tattgtacac
     3181 taactataaa acttaaactt tctatgaaga aagaatatct gtaagacttt caaggaaagc
     3241 attgaccatc tcaggaaagt ttattcagtt accatcataa acaaaatttt ttgatagaat
     3301 aaatataaaa tttttttgaa agttcctttc cacatttttg tgtgaatttg acttaatgct
     3361 gagaatctgg cttttgaaca ccttgaatta gaggttgtga caaaaatctt gtcataacct
     3421 cttttggcac atatatttgt ccttctattc taatttttga cgaacaaccg ttaaataatg
     3481 ttgcaaacca accgtcatta cccacgcaac acctaaaccg atgaaacttc ctatgacgat
     3541 ccctaaaaaa cgatattcga tcaaagaaat caagtatgct ccacgaataa gtgtagatag
     3601 catcaacgcc atcggattaa gaaaaatatt agcaactgca taatttctag caactagaaa
     3661 ttgtgcgatt ccatatagaa aagtgatcag aaaaatggtc tgcaactgcg taaacggcac
     3721 aaatgacaag aatgccgaca cacataaccc acccatcgat ccaatcagat attggacttg
     3781 tcgatgcttc atcgcatcca agttttctgc tagcaaaatc gatgcacatg acaaggtcaa
     3841 ccaataagga ttatgcagat tcaagccgtg actgacataa acggaaagaa ataaggcagc
     3901 gctgtagaaa acagaatcaa tgatcactaa aggctcttcg tgaaatcttt cttttaaagt
     3961 tgctttttca attgtctgct caggccgtga atccagtaat tttgtaatca ctgccgcaat
     4021 cagggcaaaa acgacaccca taaaaaaata aagtgagacg atcggtaatt gagcgattgg
     4081 cagctgcatg ctcgttccca tggcagaaag catcgcaaaa aacaagccac ctggtttaga
     4141 aatatggaat aatcgcaaca cgaatctgct ggaaaaagca accaaagcta cggcaaatgg
     4201 ttcaagccat aaaacataag tcgataagat ccccaaaacg aaagcaacca acagtccgca
     4261 accgatcaga aacatacgtt tcagcaaata tttcatcggt atgttttgat aatagaaaaa
     4321 agtaaagatc cctaatgatg aaaaacttga aatcaaaaaa tcatgattta tgt
//
LOCUS       DAZKCE010000155         4336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000155 DAZKCE010000000
VERSION     DAZKCE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.158"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..957)
                     /locus_tag="ACG8KQ_002707"
     CDS             complement(61..957)
                     /locus_tag="ACG8KQ_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9105594.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(950..1630)
                     /locus_tag="ACG8KQ_002708"
     CDS             complement(950..1630)
                     /locus_tag="ACG8KQ_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9105595.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1631..2413)
                     /locus_tag="ACG8KQ_002709"
     CDS             complement(1631..2413)
                     /locus_tag="ACG8KQ_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9105596.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2406..3323)
                     /locus_tag="ACG8KQ_002710"
     CDS             complement(2406..3323)
                     /locus_tag="ACG8KQ_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9105597.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3487..3741)
                     /locus_tag="ACG8KQ_002711"
     CDS             complement(3487..3741)
                     /locus_tag="ACG8KQ_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105598.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1272 a          948 c          755 g         1361 t
ORIGIN      
        1 ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa tcttgtataa
       61 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
      121 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
      181 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
      241 ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat cctcttttga
      301 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
      361 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
      421 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
      481 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
      541 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
      601 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
      661 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
      721 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc atgtattcag
      781 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
      841 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
      901 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt taatcaaatc
      961 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agcttccctc
     1021 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
     1081 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
     1141 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
     1201 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
     1261 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg atatcaaatg
     1321 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
     1381 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
     1441 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
     1501 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
     1561 gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
     1621 gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
     1681 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
     1741 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
     1801 ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa agaaaagaaa
     1861 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
     1921 tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
     1981 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg gctagtcccg
     2041 tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
     2101 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
     2161 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
     2221 ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
     2281 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
     2341 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
     2401 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
     2461 ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg attgatggct
     2521 gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac aaaataatcc
     2581 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
     2641 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
     2701 tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
     2761 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
     2821 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
     2881 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
     2941 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
     3001 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
     3061 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
     3121 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
     3181 gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt gatttcgatt
     3241 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
     3301 accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt gaagtatagt
     3361 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
     3421 tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt ttgttatacg
     3481 cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt attcctcttc
     3541 actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac cggttataag
     3601 gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac gtggttgcgt
     3661 tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg ccaccatggt
     3721 aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt ctcgctgaag
     3781 ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt atgaaaaata
     3841 atacatgccg tttgggattt acaattagct accaaataag aggttatgac aaaagacttt
     3901 tcacaatccc ttcttccgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
     3961 aatatttatc aaacatgaga agcaattttt gtaaattcct tcttatttct tgaagctgac
     4021 cacttctgtc acaacctcag aagcggataa ataatattgt ttgatgatac tttatctttt
     4081 tcccgtttgt ttatagatcg aatgatatta tagaccaaga atcctaccta ttaacatagc
     4141 taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt taggattaag
     4201 gtaactgcgc tcagcattgc ttcgcccagt acgattctcg taactcgtga actcgaacga
     4261 taatcgcaat taacgttttg agaactgtga agctttacgg gctttcttaa gacctggttt
     4321 tttcttcgta tcattg
//
LOCUS       DAZKCE010000156         4294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000156 DAZKCE010000000
VERSION     DAZKCE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.159"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..791
                     /locus_tag="ACG8KQ_002712"
     CDS             <1..791
                     /locus_tag="ACG8KQ_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9105599.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            888..3137
                     /locus_tag="ACG8KQ_002713"
     CDS             888..3137
                     /locus_tag="ACG8KQ_002713"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9105600.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3140..>4294
                     /locus_tag="ACG8KQ_002714"
     CDS             3140..>4294
                     /locus_tag="ACG8KQ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9105601.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1393 t
ORIGIN      
        1 tttgtgggta ttcaaaaaca gaacctagac atagttttgg accagctatt cgagaacaat
       61 acttaataca catagtgcta gaaggtgaag gctattattc aattaatgat caaaaatatt
      121 ttctgaagaa aggccaagga tttgttatcc caccaggagt atctactttt tatcaagcag
      181 atgaaaaaaa tccgtggtca tatgtatggt tgggtttaag tggcaatctt gtagaatact
      241 atctaaaaga aattggtgtt ggaaagaatc atcttacctt taaagtcgaa aatttaaatt
      301 catttaaagg attaattttt gaaactttat cgtatgatca ggacaatcta gtcagtgaat
      361 tgtccagaca aaaacaagtt tataaatttt tagaactcct cttaaattca gctagttatc
      421 tgaacgaagg gataattact aaaaagatga atccttttgt tcgtcaaaca attgaaataa
      481 tacgtgagaa tgcgtatcaa aaattatctg ttaaagatat tgctgtcagt ctatcgatac
      541 acgaaaatta tttatcacga ttgtttaaga atgatatggg aatatcaata aaagagtata
      601 ttagtgaaat caaaatttct attgcaaacg atctgttatc atcaactgaa tattcaatcc
      661 agcaaatcag tgacaagata ggattctcaa cagtacaatc tttttctaaa gcctttaaga
      721 aagcaaatgg tgtttcaccg ggagtatatc gaaaacgtag attaggtcta ggaatcatta
      781 atggtgaata aactaaacat ttgattttga taaatattcg ttactttaaa cgcttactat
      841 ttatgtgagc gttttttata atgcaataaa gaaacggagg agagcttatg tataaggata
      901 atgaaatgaa tattatttat cgatcagaaa accaacaatt tcatttgttt aatcaggaga
      961 taagttacat ttttaagata tcggaagatg gtaaattatt gcatttatat tatggtgaga
     1021 acttaccaga taatgactat agtcatctaa ttgagatgca tcatcggcct atgacaacct
     1081 atagaaatga aaacgatttg ctatattcgt tagagcactt aaaacaagaa tttcctgaat
     1141 atggtacaac agattttaga catcctgcta tttcattact acagaaaaat ggctctcgct
     1201 tatcagattt tgtctatcaa gggtacgaag tggaagaagg aaaacctaaa ctagaaggtt
     1261 tacctgctac ctatgttgat aataatgatg aaagcaaaac attgaaagtt tatattttag
     1321 attcagttac aaatgttcaa gtagaattat tatatacaat ttatagagat tatcctgtta
     1381 ttactcgtag cgcgcgagtt atgaataaag gaaaagatat tcaaataatc gagaatatta
     1441 gcagtttatc attggattta cccgatgcaa attatgaatg gcaacaatta tctggtgctt
     1501 ggggtagaga aagaactatt aagtcgcgta ctttacaaca aggaattcaa tcaatcgaat
     1561 ctactcgagg tatttcaagc catcagcata acccatttgt tactttaaaa agaaaaaata
     1621 ctgatgaaaa tcaaggagaa gctattggtg cagcactagt ttactcagga aacttcttaa
     1681 ttcaagcaga agtagataca tgggatgtta ctagactaca agttggcatc aatccatttg
     1741 gatttaattg gaaactcaat ccaggagaaa tatttacagc accagaagcg attctggttt
     1801 attcaagtaa gggtttaaat gccatgagtc aaacttttca tcaactattt agaaaacgct
     1861 tagctcgtgg taagtggcga gaaaaagacc gtcctgtctt aattaataat tgggaagcaa
     1921 catattttga ttttgacgaa gaaaagttgg taaatattgc taaaaaagca tttgatgttg
     1981 gtattgaatt atttgtatta gatgatggct ggtttggaga aagagaaaac gattgtgcag
     2041 gattaggaga ttggcatgta aatcctaaaa gactacctag aggaatcggc agcctttcag
     2101 aaaaaattcg tagttttggt atgaaatttg gtttatggtt tgaacctgaa atggtgaata
     2161 aggatagtga gttatatcgc acacatccga attggattat ttctacacct aatcgaaaac
     2221 ctaatcatgg taggaaccaa tatgtattaa attttgggtt agatgaggtt gttgaaaata
     2281 ttttcgatca aatgtgtaaa atcattgatg aatcaaactt agattacatt aagtgggata
     2341 tgaatcgtcc attgactgat gtatttgaca gtcacttgtc agcagatcaa caaggagaag
     2401 tattccacag atatgtacta ggcgtatatc gcttatatga aaaacttata acaaaatatc
     2461 ctaacatact atttgagtct tgttcatcag gtggtgggcg ctttgatcct ggaatgcttc
     2521 actatgcacc tcaagcttgg gctagtgatg attcagatgc aattgaaagg ttaaaaattc
     2581 aatatggaac tagtatgctg tatcctcttt cgtctattgg tgctcatgta tcaattgttc
     2641 caaatcatca aacaaaccgt ataacaccta taaaaactag aggaaatgta gctttttttg
     2701 gtgcatttgg atatgagctt gacttaaatg aattaagtga aggcgatttg aggattgtaa
     2761 aagaacaaat tgccttttat aaaaaatata gaaccataat tcataatggt actttttata
     2821 gactattatc accattcgat gatgataatc aaaccgcgtg gatggttgtt agtcatgata
     2881 agaaaacggc tattgttgct cattataaaa cattaaatga ggtaaatgct ccttaccgcc
     2941 gactgaaatt aaaagggtta gataatgacg gactttataa tgtaggacaa aatagtttgc
     3001 gatctggaat ggaattgatg agagcaggac tagtctgttc agatgcatct agtggacaaa
     3061 taaatgatca gaatcgacgc ccagaaacat ttgattttga ttccaagatt tgggttataa
     3121 cacaggagga aaagtagaga tgaataacca aaaaagctta cttcaaaaag gagcaatctt
     3181 aagtatttca ctaatgttaa caagctctca agcaattaat ggtgttattc cacaaatgaa
     3241 acagtctttg gggatatcac aaagtcaaag tgaactttta ggaactgcac caagtataac
     3301 tgttattttg tttatcttat tatctagtta ctttgccaaa agaataggaa tgaaaaagac
     3361 aataatttta ggtttattat tagctggtat aggaggaatt attccagtat ttacagctac
     3421 cttccaaacc gttcttatta gtcgagtaat attaggtgcc ggtttaggag tatataattc
     3481 attggctgtg acatatatca gctctttata ctctggcaat actagagcaa cgctattagg
     3541 tattagaaat tcaatggaag ctttaggtca gacaatcctt atttttcttg ctggagtttt
     3601 agtgaacatt aattggacgg cttcgttttt agtctatgca attgcgtttc ctgtagctct
     3661 cttattcgcg ctaaaagttc ctgaaattag agatgaaaat agtgacatac ctgaaaatca
     3721 tgtaaaagaa aagatgaatc caatggtttt tgccctagtt ctatttgcaa tcttactcgt
     3781 aatgaatagt attgctattt ctgtacgatt tgcatcaatt gcaactgaaa ttaaaggggt
     3841 aaactttaat gctagtaact atttagctct aatgcctatc ttaggaatac tagcaggatt
     3901 tatgtttgga ccaataaata agtggatagg aaaaggaacc ctatacttgg gaattatatt
     3961 ctttataatt tcaaatttgt taattgctgt gtcaaacggt aatatgacgt tcctgctaac
     4021 gggtttattt ttatcaagta taacaggatc atggtgcttc ccatttatct ttagtaactt
     4081 agataaagtc actacaaaga atacaattaa ttttgctact tcattgattt ttattggctg
     4141 taatattggt aattttatag ctcctattgc tatgcaatta gcacaatttc taacaagaag
     4201 tacaaatttg actgctccat tttttgtatt cgcaggaata tttattgtga tcctatttgt
     4261 aacatttttt gcgacaaata aaaaaaatac taga
//
LOCUS       DAZKCE010000157         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000157 DAZKCE010000000
VERSION     DAZKCE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.160"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8KQ_002715"
     CDS             complement(37..1257)
                     /locus_tag="ACG8KQ_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9105602.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8KQ_002716"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8KQ_002716"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9105603.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8KQ_002717"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8KQ_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9105604.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8KQ_002718"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8KQ_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105605.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKCE010000158         4262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000158 DAZKCE010000000
VERSION     DAZKCE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.161"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8KQ_002719"
     CDS             complement(<1..149)
                     /locus_tag="ACG8KQ_002719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9105606.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8KQ_002720"
     CDS             complement(593..1714)
                     /locus_tag="ACG8KQ_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9105607.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8KQ_002721"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8KQ_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFP9105608.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8KQ_002722"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8KQ_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFP9105609.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1408 a          769 c          806 g         1279 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 ga
//
LOCUS       DAZKCE010000159         4200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000159 DAZKCE010000000
VERSION     DAZKCE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.162"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..437
                     /locus_tag="ACG8KQ_002723"
     CDS             <1..437
                     /locus_tag="ACG8KQ_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105610.1"
                     /translation="EWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVL
                     VLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLC
                     FYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            532..1416
                     /locus_tag="ACG8KQ_002724"
     CDS             532..1416
                     /locus_tag="ACG8KQ_002724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9105611.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1638..>4200
                     /locus_tag="ACG8KQ_002725"
     CDS             1638..>4200
                     /locus_tag="ACG8KQ_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9105612.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1364 a          709 c          952 g         1175 t
ORIGIN      
        1 tggagtggtt ttgcaaattt cttttatttc tagaagttat tcacgtatac ggatttgttt
       61 ttctcacaaa taaaaaggca ggaagtacaa tggatcattt gtatataaaa tatattcatc
      121 aattttccgt tttggtatta ggcacactaa ttatagcggg gattgttgca tttattattc
      181 aaggtcgtga catattaaca gtacatcaag agtttttcag ttatgcattt ggtattcctc
      241 tgatcgctat aaggtctgat aacaaaaagt ggaagaaacg ctattacatt ctcttgctat
      301 ttttgtgttt ttatctgcca cctaagattt ttactttatt aggtatacaa tgcctatcat
      361 tttcttttat ttcctatatc attgttttaa ttttcctcag tttcatagta tgggtatttt
      421 atcttgtcaa aaaataaatg aatgtttcat gaacagtact tcatcacagc ctaatttttt
      481 tctttatgct atcgttatat taaagaaatc aatcagaagg gaaagaaagt gatgaaaaaa
      541 gttttattag tggtaaaccc cagttcaggc ggtgaacaag caaaggaatt tgagcaatta
      601 gcgatagcaa aattggaatc ggtgtttgat gaagtggtcg tcttgcatac aaaaaaagca
      661 ggggatgcaa aaaactttac tcgcgaagcg gctacggaag ggtatcatag tgtgtttgtc
      721 atgggcggag atggaacagt caacgaagga atcagtggaa tcgctgaaca agaacatcgg
      781 ccaaatttcg gatttttccc attgggtacg gtgaatgact tggctcgagc attagggata
      841 ccgctagaac ctgaggaagc gatcaatcat ttttcgattg aatcagtcaa gccattggat
      901 attggaaaaa taaatgacga ctattttatg aatgtcgtcg caattggctc tatccctgaa
      961 gcaatcaatg atgtagatgc ggagaagaag acgaaatttg gaaaactagc ttatttcatg
     1021 tcaggaatca aacagttagc ttccacacaa tcctactcgt ttcacgtaga agttgatgga
     1081 aagaaggaag aaattgagag cagtatcttg ctgataggac taactaactc agtaggcggt
     1141 tttgaaacat tacttccaaa tgcaaaagtg gatgatggta agctccattt ggtttattta
     1201 aaagattctt ctttgatcga tacgcttaaa acattgccag acctattaaa aggagtagat
     1261 gaatctacga aaaatctagc ctatcttact tgtgagcaat tagttgtgtc attagctgat
     1321 gaggaagaat tgacgacgaa tgttgatgga gatgaagggg ataaattacc tgtgacaatc
     1381 aaagtgttac cctcacattt acaggtttat tgttaaaatg gaggttgtga ccgaagagtt
     1441 gtcaggagga catcggttgt tcggaataag ggactgtgac aagacttttg tcacagcctt
     1501 tttttgtaca aaaaagaaat tttcacctaa cagaaaaatt tgccataaaa atacatattt
     1561 taaatatgaa agtacaatta caaataaatt aaaacgttaa aataaaattg tcaaaacgtc
     1621 agagaggatg gtggagaatg aatcaatatg taaaaaagct gattgcgtcg attttgtttg
     1681 tcttccctct cttgatggtg acatccgttt ttgccgatgt agagaaaaca agcgaatcaa
     1741 cgcaatcaga gaatcaaagt tttgacaaga ctactttttc agaagaatca attacttctg
     1801 aaactacaga atcccaaagt gaggcgccta cttctaatac aaggcaagaa gaaaataaga
     1861 caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagag gtacaggtta
     1921 gagttgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg acgcagcttc
     1981 tcgcagacga atcagtagct aagattcgtt taactcagcc tttgacttta gacagggaat
     2041 tggagatcaa gcgagatatc gttatcgatt ttggcggact tgctcataat ttcggcacac
     2101 atcacatcta tatcaatgag acacctcagt cgatcgagtt tcagaatttt aaaggaacgg
     2161 cagctcatcc aggtgggttg ataccaaata tcgatggaaa tgccattatc attgcttata
     2221 tgtccgattt ttggggaaac agatatcatt ttacgggaga aataaaattt accgggacac
     2281 ttgacttata tgatggttct aaattaggcc tgatttacgc ccctcgtgca actgtcacat
     2341 tagatggtgt ttccggtgta ttggatgtcc aaccagtgaa agaagggatg aatgccgctc
     2401 cgggtaaagc ttatttttgt agaagctatc agctaaatgt aatcaatggt agtcagcttt
     2461 atggaccata tctaggaaaa ttctatggtt ttctagattc aggtgatgag acgggcagtg
     2521 gaaaaacagc accaggactc catatcttga gtggttcaaa ggtctctctt gactatgatc
     2581 gggcagatgg cgtcagcgat gaaggagagg ctgtcgatac gctacccggg aatgtcacat
     2641 ttaaagtcag tggctcagga agtgaatttt cggtaaatac aaagatcaat attaccaacg
     2701 acaacaatcg cggaattatc caaatgagag gctcaggatc aagagtcgct gtgtcgaatg
     2761 atgggaaaat cacaatcaat acagcgacta ctggcggatt tcgcctgcaa ggagattcta
     2821 gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga gatggcgacc
     2881 aacctggaaa caatgggatg cgttttgtag ggaaaggact ttctttgatc gtagaaggag
     2941 ccggatcagt catcgatatt gataagcaaa gtggtcgttc tagcgcagtt cgttttgaga
     3001 acggtacgca aaaactaaca gtaactaatg gaggctcatt gaaggtaaga aatgcagggg
     3061 atggcaaatc ttatgtgaac agactcaatc aaggaaacca agcgatccaa ttttatgacg
     3121 cttctggttt tctagaaagt ccagggtcag ctgattttac ggttactgga gaaaaatcaa
     3181 atatcgatat acgcgcagat tccggagcta ccgtagatac aactggagat attgatttga
     3241 actttatggc tggcgaaaaa acttatttag tcatggtcgg gaaaactggg gaagataaga
     3301 ctggcatcat cagtggggat gtgttgaatg tagacttaga atctcctacc tatttcgatt
     3361 tccaaaacaa acggatcgga agcgagacta atggcatagg tggctgggta tttcaaggaa
     3421 atggcgattc ttctttgaag atcgctcatt cagaaattgc tttatggcga aaggcaggag
     3481 taacgttcga taactttgaa aaagatccgg attatttttc tgatatgaca gacttatcga
     3541 tcaatggaac cgacttaggt aattttgtat caagccctag tgcaggttta acaaatgaat
     3601 ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat aaccagcttc
     3661 cgatgattga atccttaaga gtaccaacga atgcagataa aaaaatctat gggcatgtcg
     3721 ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga
     3781 ctttacgtat tacttatgca gatacgacga aatcacctgt agaaattact gctctcacac
     3841 aaggacaaac agatgaaaat gaagggacag gatataatcc gtggggagaa ggcgaacaag
     3901 gcggtttgtt tgaggttcaa gtacccaacg aagaaagact aacaacgggg gatcaagtgc
     3961 aaatagttgc tgctaaaaaa gtaagaggcg gccaagaagt gacagaactg gactctgcca
     4021 aaacgacagt cgatgtgatt cctcctgagc ctgctaaatt aagtaccaca ttgatcaatg
     4081 cagcttctcg ctcaatcaca ggtacaggcg aagcaggagc tttggtttca ttgaaaaaaa
     4141 atcaagctgt tttgtccgaa acaactattg atgatacagg taaatttgtt ctttctatcc
//
LOCUS       DAZKCE010000160         4181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000160 DAZKCE010000000
VERSION     DAZKCE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.163"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..125
                     /locus_tag="ACG8KQ_002726"
     CDS             15..125
                     /locus_tag="ACG8KQ_002726"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105613.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            130..2259
                     /locus_tag="ACG8KQ_002727"
     CDS             130..2259
                     /locus_tag="ACG8KQ_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9105614.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2256..3263
                     /locus_tag="ACG8KQ_002728"
     CDS             2256..3263
                     /locus_tag="ACG8KQ_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9105615.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3280..>4181
                     /locus_tag="ACG8KQ_002729"
     CDS             3280..>4181
                     /locus_tag="ACG8KQ_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9105616.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTIS"
BASE COUNT         1381 a          673 c          950 g         1177 t
ORIGIN      
        1 aaaacggtca atgcttgatg caagacttgt atgggcgagt aggtgtggtt caagttcatc
       61 cagtctttga agaactcttc catgcttttg acactcgtcc gccggttcaa gagagaatgg
      121 agtgagggga tgaaaaagaa actttttcgg attgtgggaa tcgtttttat tagcatcgtc
      181 ctattgacag ttctattgag tttagtcggt acggtagctg aggcgacagg tctggttgat
      241 gatacagttg aagccggaaa cctttattcc aaatattctc tgaacaatta tcaactggat
      301 ttctttgtgg atagttcttg ggattggtta ccttggaatt ggggggatgg cttagggaaa
      361 agcgtgatgt atggtcttta tgccattacc aattttattt ggacagtaag tctgtattta
      421 tcaaacgcca cgggctatgt ggttcaagaa gcttacaagt tggatttcat ttcggatacg
      481 gctaaaagta ttggaaagaa tatccaaact ttagccggta tcactgaaaa tggattacag
      541 acttcgggat tctactttgg gttcctatta ttgatgattt tagctctagg agtttatgtg
      601 gcgtataccg gattattaaa acgagaaact accaaagcag tccgaaccgt cattaatttt
      661 gtgatgattt ttctactctc gggttctttc attgcgtatg cgcccaccta tattacaaaa
      721 atcaatgact ttagctcaga cgttagtgaa gcggctctta gccttggaac caagattgtt
      781 gtacctaatt cagaaagcca gggaaaagat agtgtggatt tgattcgaga cagtttattt
      841 tcgattcaag ttcaacaacc ctggttacta ttacaattcg atgattccaa tatagaagaa
      901 attggcgagg gtcgggttaa taagatttta tccgtgagtc cggatgaaaa taagggtaag
      961 gatcgggaag aagcagtcaa agcagagatt gaagataatg acaatgcgaa tctcagtatt
     1021 accaagacca tgaaccgttt aggaaccgtg gtctttttag tgttgtttaa tattgggatt
     1081 tccttttttg tgtttcttct gacagggatt atgttgttct cacaaatcct tttcattatc
     1141 tttgcgatgt ttctgcccat tagtttctta ttgtcgatgt tgccgacgta tgagagtttg
     1201 ggcaaaaggg cgattattcg tttgttcaat acgattatga tgcgagcggg agttacctta
     1261 gtcattacga ctgcattcag tatctccacg atgtttttca atatttcggc cacctatccc
     1321 ttctttatgg tagcttttct acaaattgtg acctttgccg gtatctattt caaattaggt
     1381 gacattatga gcatgtttaa ccttcaaagt aatgatagtc agtcaatggg aaggcgagtc
     1441 atgcggaaac cgcaaatgtt gatgaatcga aaattgcgac agcttaatcg aaatgtgggt
     1501 cgaacttttg cttttggtgg cggtgcagca gttggtggga aactagctac cagttctaca
     1561 aaagggaaag gtagttcaaa aatcaataga gtgtccaata gaaagaacca ttccgcgaga
     1621 agcattaacg agctaaattc tgatctgaaa aaacaagaac aaccagtggg cacgaagaaa
     1681 gaatcacgaa taaatttagt tggtagaaaa gttggaaaag tactcgatac aaaagaaatc
     1741 gtgaaggaca aagcgaaaca agcaaaaaat caagtaaccg atgcaccaac taacttgaaa
     1801 tacagccttc ataaaggcat ggaaaagacg aaaaaagtac cagaggactt taagcgaggg
     1861 cctttcgaag aaaaagcaaa tcgaggggag ttgcgagaaa aacaacgaca acgaagagac
     1921 gcaaaaatgg atgaaaaacg caaagtattg aacgaagcat ggaatcgtca tgagaaagga
     1981 agggaaaatg ttccgataaa aaaggagata aaaccacaga aaaatgaact accagaaaat
     2041 aggtctttaa aaccaagggt acaagtacat tcgaatcctg aaatcaaacg gaaactttct
     2101 agtcaagaag tcattccaaa cggaagcact cagccactaa gcaagaacaa ttcctttcaa
     2161 caagtgaaac agagattcac ttttccaaag cgaaccaatc aacaagtaca aagaaaaaag
     2221 aacactcgcc caaaaccaaa gccaggtgag aaaagatgaa cttaaaaaaa ttgagtatta
     2281 tttcgttaac cttcctgacc atctcttttg tgggcatctt gatgtgtgtg ggtttgttgt
     2341 ttggtgagga cggtgaggga agtggtagtt ctggaacttc tcctggaggt agtagtgtct
     2401 cagaagtagt tttgaaacac cgttcaatgg tggaaaagta tgccaaagaa tcagatataa
     2461 cagagtatgt ttcgattctt ttggcgatta ttcaagtcga atctggaggt acaatggaag
     2521 atgtcatgca aagttctgaa tcagcaggtt tatcaccgaa ttcattaagt accgaggcat
     2581 ccatcaaaca agtgtgtctc tatttttcgt ctttggttaa gtgttccaaa gaactaggat
     2641 gcgatcaaga tagtatcatt caggcttata actatggtga cgggtatttg gattatgtcg
     2701 cgaaaaacgg aaagaagcat agttttgctt tagccgaatc tttttcaaaa gaaaaatcgg
     2761 gtggtcaaaa agtcgattat cccaatccca tcgcgattaa ggaaaatagt gggtggcggt
     2821 ataactatgg caatatgttt tattgcttat tagtgaagca gtacctaact acaacacagt
     2881 ttgatgataa aacagtccaa ggaatttttg acgaggcgtt caagtatgaa ggaacggctt
     2941 atgtatttgg tgggtcaagt cccggaactg ggtttgattg tagtggttta actcaatggt
     3001 gttatgccaa agttggtttg aaactccctc gggtggctca ggatcaatat gatgctatga
     3061 ctcatattga cttaaaagat gccaaaccag gcgatttgat tttctttcat tcaacttatg
     3121 atgccggtac ttatgtgact catgtgggca tttatgcagg agagaacaga atgtttcatg
     3181 caggagatcc ggtgggttgg accaatctta ccgatagcta ttggcaacag cacattattg
     3241 gtgctggtcg gtataaacaa tagaaaagag gaatggaaaa tgaagataaa aattgagcga
     3301 agtaacaagg aaaagattcc aaaaaagaag aaagaacgag tagtatccgt gggcaagcat
     3361 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gccttgcttt tggaatctac
     3421 aaaaacttca cggccattga tcagcataca actcatgaga aagtggtcgt gaaagagaaa
     3481 atagtgaata cctctggagt agaaaatttt actgtagatt ttgtgaaaga atatttttca
     3541 tggaagaata acaaggaggt aattgaaaag agaatgacta atcttgagca gtaccttaca
     3601 gaagaagggc tcgctttgag ccaagatatg gttcgagcgg atattcccac tagttccgag
     3661 gttgtatcag taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
     3721 gtggtagatc aaaagattac agaagggaaa aagacacaat caatttcttc tgcatatcgt
     3781 ctgatgatct ttgaagatga aaaaggcaat catattgtaa cgagtttacc cactatgatt
     3841 ggtaagccag aaaaggcaaa acataaagta agacaagtaa aaagtgattc aaaaattgat
     3901 tcaaagacaa cggaagaaat caccgagttt ttagagacgt ttttcaggct ttatccagca
     3961 gcctcagaaa aagaactaga gcattatgtt gaaaaagacg cgatgcaacc aattaatact
     4021 aatttgaaat ttgtggaatt agtgaatcct atttacaaac aaaagggtaa agcgattcaa
     4081 gtgattgttg gtgttgagta tcttgatgag ttgtccaaaa caacaagtgt ttttcagtat
     4141 agattaaaac tatacaaaaa tgaaaattgg actattagca a
//
LOCUS       DAZKCE010000161         4161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000161 DAZKCE010000000
VERSION     DAZKCE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.164"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..691
                     /locus_tag="ACG8KQ_002730"
     CDS             <1..691
                     /locus_tag="ACG8KQ_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9105617.1"
                     /translation="GRNNVMFTLSLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVI
                     KIVRSAYSENYQGANREYITILCKAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLS
                     EWKEDLMAYISEKSDVYKPYLVTTKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKP
                     GRNGGIQLASVKSLLLSIIKVKKEEKESYIKALTNSFDLEHTFIQETLNKLAERPKTD
                     TQLDLFSYDTG"
     gene            1039..1209
                     /locus_tag="ACG8KQ_002731"
     CDS             1039..1209
                     /locus_tag="ACG8KQ_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105618.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1223..1840
                     /locus_tag="ACG8KQ_002732"
     CDS             1223..1840
                     /locus_tag="ACG8KQ_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9105619.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            1840..2778
                     /locus_tag="ACG8KQ_002733"
                     /pseudo
     CDS             1840..2778
                     /locus_tag="ACG8KQ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(3471..4118)
                     /locus_tag="ACG8KQ_002734"
     CDS             complement(3471..4118)
                     /locus_tag="ACG8KQ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFP9105620.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1517 a          636 c          726 g         1282 t
ORIGIN      
        1 agggcgtaat aacgtcatgt ttaccctctc tttagcctac tttagttcag gctattcaat
       61 cgaaacgtgc gaatataata tgtttgagtt taataatcga ttagatcaac ccttagaaga
      121 aaaagaagta atcaaaattg ttagaagtgc ctattcagaa aactatcaag gggctaatag
      181 ggaatacatt accattcttt gcaaagcttg ggtatcaagt gatttaacca gtaaagattt
      241 atttgtccgt caagggtggt ttaaattcaa gaaaaaaaga agcgaacgtc aacgtgttca
      301 tttgtcagaa tggaaagaag atttaatggc ttatattagc gaaaaaagcg atgtatacaa
      361 gccttattta gtgacgacca aaaaagagat tagagaagtg ctaggcattc ctgaacggac
      421 attagataaa ttgctgaagg tactgaaggc gaatcaggaa attttcttta agattaaacc
      481 aggaagaaat ggtggcattc aacttgctag tgttaaatca ttgttgctat cgatcattaa
      541 agtaaaaaaa gaagaaaaag aaagctatat aaaggcgctg acaaattctt ttgacttaga
      601 gcatacattc attcaagaga ctttaaacaa gctagcagaa cgccctaaaa cggacacaca
      661 actcgatttg tttagctatg atacaggctg aaaataaaac ccgcactatg ccattacatt
      721 tatatctatg atacgtgttt gttttttctt tgctgtttag cgaatgatta gcagaaatat
      781 acagagtaag attttaatta attattaggg ggagaaggag agagtagccc gaaaactttt
      841 agttggcttg gactgaacga agtgagggaa aggctactaa aacgtcgagg ggcagtgaga
      901 gcgaagcgaa cacttgattt tttaattttc tatcttttat aggtcattag agtataccta
      961 tttgtcctaa tatgatttta gcagtataat tgacttggtg aataggtcat ttaagttggg
     1021 cataatagga ggagtaaaat gaaaaaattt atttatcgag ttttagaaaa tgacgaagtg
     1081 gtggctattt ttaatgagca acaatatgcg caagatttta tcgcttacga aaagacaatt
     1141 tctgataagc aatttgaaat tgaaaaagta gatattgctg attggttatt gcaaccgaga
     1201 gaattttaga ggttggttga aaatggctaa aattggttat gcacgtgtca gtagcaaaga
     1261 acagaactta gatcggcaat tacaagcgtt acagggcgtt tctaaggtct tttcagacaa
     1321 attaagcggt caatcggtcg aacgcccaca attacaagct atgcttaact atattcgtga
     1381 aggggatatt gttattgtta ctgaattaga tcgattagga cgaaataata aagaattaac
     1441 agaattgatg aatcaaattc aaattaaggg ggcaaccctg gaagtcttaa atttaccctc
     1501 aatgaatggt attgaagatg aaaatttaag gcgtttgatt aatagccttg tcattgaatt
     1561 gtacaagtat caagcagaat cagaacgaaa aaaaattaag gaacgtcagg cacaaggaat
     1621 cgaaattgct aagaaaaaag gcaaattcaa aggtcgtcag cataaattta aagaaaatga
     1681 tccacgttta aagcatgctt tcgatttgtt tttgaatggt ttatccgata aagaagttga
     1741 agaacaaact ggaatcaatc gccgaacgtt tagaaggtat cgagcaagat ataacgtgac
     1801 agtcgatcaa agaaaaaaca atgaaaagag ggatagttaa tgagtacggt tattttagct
     1861 gaaaaaccaa gccaggcatt agcctacgca agtgctttaa aacaaagcac caaaaaagac
     1921 ggttattttg agatcaaaga cccactattt acagatgaaa cgtttatcac ctttggtttt
     1981 gggcatttag tggaattagc agaaccaggt cattatgacg aaaagtggca aaattggaaa
     2041 cttgaatctt tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga
     2101 aagcagttta aaattgttgc agaacttctc aaaaaggcaa atacaattat tgttgcaaca
     2161 gatagcgaca gagaaggtga aaatatcgcc tggtcgatta tccataaagc aaatgccttt
     2221 tcaaaagata aaacatttaa aagactatgg atcaatagct tagaaaaaga tgtaatccga
     2281 agcggttttc aaaatttgca acctggaatg aattactatc ccttttatca agaagcgcaa
     2341 acacgccaaa ttgccgattg gttgatcggc atgaacgcaa gccctttgta tacgttaaat
     2401 ttacaacaga agggcgtaca aggtacattt tcactaggac gtgttcaaac gcccacctta
     2461 taccttattt ttcagcgcca ggaagccata gagaatttta aaaaagaacc ttttttcgag
     2521 gtggaagcta gtataaaagt aaaccaaggg tcgtttaagg gcgttctaag ccccacacag
     2581 cgttttaaaa cccaagagga gcttttagct tttgtttctt ctaaacaagc taaaataggc
     2641 aatcaagagg ggataattgc tgatgttcaa accaaagaga agaaaacgaa tagtccgagt
     2701 ttgttttctt taagtagttt gcaatcaaaa gtcaatcagc tttataaagc gacagcgagc
     2761 caaactttaa aagctatttc ttttttaata acttaaaaat aaacttaatg taacagcaag
     2821 cacagtcaag gtatacacct ttgacaaaaa atagcacatt ctctatcgaa aatttttgct
     2881 tattttttaa attattttgg gaaattttcc caatcccttt ttctaactca aaaaatataa
     2941 tcactcaaaa tttaaaaggg cgcacttata catcatttta aaaaattgat gtaacgtgct
     3001 aagttcaaaa caaagggcgc acttatacac gattttcaat cttgtatatt tctaacgaaa
     3061 agcgtgcgcc aaaaaacccc cttcgtcaat tttgacaggg ggctttttga tgtaaaaatt
     3121 tctatcgaaa tttaaaaatt cgcttcactc atgttataaa gacttaaaat aaaataactc
     3181 tttaaaatct tttgctagtt gttcttcaat attttttatt cggtgcatct tccaagtaaa
     3241 gtataacaca ctagacttat ttactacgtt tcataagtca ttaatgcgtg tgctctgcga
     3301 ggctagtttt tgtgcaagca caaaaaatgg actgaataaa tcagtccata agttcaaaac
     3361 caaattcaaa atcaaaacca caagcaacca aaaaatgtgg ttgttatacg ttcataaatt
     3421 ttatgatcac ttacgtgtat aaaattaaat tcactttcaa aatctaaaaa ctaaatccaa
     3481 tcatctaccc tatgaattat atcttgaaat tcattcataa atagtgaagc atggtaacca
     3541 tcacatacag aatgatgaag ttgcagagca actggtatat aaattttatt attctcacta
     3601 taaaatttac ctatcgtaat aataggcaat aaaaagctgc tattgttacc aatatttaaa
     3661 ttaaatgaac taaaatcaat ccaaggaatc attgaaatcg gtatggtgtt ttcaggtatc
     3721 ggttttttag gaaacatttc ttctttatct ttatattcaa gcaagtcatt tttataatta
     3781 ttataaaaag aaatgaagtt tttatcagat tcagtccaaa tgttagtaaa tttttcagtt
     3841 tgcttattaa aaactgtata caaaggattt aacttatccc aataacctaa tttattctca
     3901 ctattaattc ctgttctaaa cactttattt ttatttacaa cttccataat tgcataaatt
     3961 aaagagggat aaatttcata tcctttcttt tttatcatat ctttaaacaa agtaatatca
     4021 atttctttag taatgctata agtagtttgc tgattaaaat agtgttcaaa atattctttt
     4081 ctatcccaat tttctaattc aataatatta aaagtcatat ataacttcct cctaaatttt
     4141 aaatttttat atttaggagg a
//
LOCUS       DAZKCE010000162         4090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000162 DAZKCE010000000
VERSION     DAZKCE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.166"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..1704)
                     /locus_tag="ACG8KQ_002735"
     CDS             complement(199..1704)
                     /locus_tag="ACG8KQ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9105621.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1701..2387)
                     /locus_tag="ACG8KQ_002736"
     CDS             complement(1701..2387)
                     /locus_tag="ACG8KQ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9105622.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2580..4001)
                     /gene="gndA"
                     /locus_tag="ACG8KQ_002737"
     CDS             complement(2580..4001)
                     /gene="gndA"
                     /locus_tag="ACG8KQ_002737"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9105623.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1129 a          861 c          695 g         1405 t
ORIGIN      
        1 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
       61 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
      121 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
      181 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
      241 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
      301 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgcattatc
      361 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
      421 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
      481 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
      541 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
      601 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
      661 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
      721 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
      781 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
      841 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
      901 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
      961 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     1021 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     1081 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     1141 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     1201 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     1261 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     1321 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     1381 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     1441 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     1501 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     1561 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     1621 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     1681 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     1741 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     1801 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     1861 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     1921 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     1981 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     2041 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     2101 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     2161 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     2221 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     2281 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     2341 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     2401 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     2461 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     2521 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     2581 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     2641 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     2701 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     2761 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     2821 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     2881 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     2941 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     3001 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     3061 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     3121 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     3181 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     3241 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     3301 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     3361 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     3421 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     3481 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     3541 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     3601 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     3661 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     3721 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     3781 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     3841 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     3901 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     3961 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     4021 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     4081 caacatattt
//
LOCUS       DAZKCE010000163         4040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000163 DAZKCE010000000
VERSION     DAZKCE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.167"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACG8KQ_002738"
     CDS             178..774
                     /locus_tag="ACG8KQ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9105624.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACG8KQ_002739"
     CDS             898..2697
                     /locus_tag="ACG8KQ_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9105625.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACG8KQ_002740"
     CDS             complement(2975..3187)
                     /locus_tag="ACG8KQ_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105626.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACG8KQ_002741"
     CDS             3649..3798
                     /locus_tag="ACG8KQ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105627.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1189 a          689 c          711 g         1451 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata
//
LOCUS       DAZKCE010000164         3866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000164 DAZKCE010000000
VERSION     DAZKCE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.169"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..469)
                     /locus_tag="ACG8KQ_002742"
     CDS             complement(248..469)
                     /locus_tag="ACG8KQ_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105628.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="ACG8KQ_002743"
     CDS             complement(567..812)
                     /locus_tag="ACG8KQ_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105629.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="ACG8KQ_002744"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="ACG8KQ_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="ACG8KQ_002745"
     CDS             complement(2036..3037)
                     /locus_tag="ACG8KQ_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFP9105630.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="ACG8KQ_002746"
     CDS             complement(3196..3462)
                     /locus_tag="ACG8KQ_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105631.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="ACG8KQ_002747"
     CDS             complement(3452..3808)
                     /locus_tag="ACG8KQ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9105632.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1434 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaatt
//
LOCUS       DAZKCE010000165         3836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000165 DAZKCE010000000
VERSION     DAZKCE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.170"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(583..1140)
                     /locus_tag="ACG8KQ_002748"
     CDS             complement(583..1140)
                     /locus_tag="ACG8KQ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105633.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1319..1930)
                     /locus_tag="ACG8KQ_002749"
     CDS             complement(1319..1930)
                     /locus_tag="ACG8KQ_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105634.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2043..2300)
                     /locus_tag="ACG8KQ_002750"
     CDS             complement(2043..2300)
                     /locus_tag="ACG8KQ_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105635.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2897..3175)
                     /locus_tag="ACG8KQ_002751"
     CDS             complement(2897..3175)
                     /locus_tag="ACG8KQ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105636.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3209..3460)
                     /locus_tag="ACG8KQ_002752"
     CDS             complement(3209..3460)
                     /locus_tag="ACG8KQ_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105637.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1156 a          788 c          509 g         1383 t
ORIGIN      
        1 cccagcctcc ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca
       61 tttcttatga caagctaagc aaattatctc caccaaataa tttattcata tttttgactt
      121 gcgcatacta actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta
      181 atatttatgt acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc
      241 aattctactt caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg
      301 ttgagctagg tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac
      361 caaacgcaag atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat
      421 aaagctaaca actattaact cctctcagct aatagttgct ctctttttta ttcagattca
      481 tttttgctga tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat
      541 tacaacattc actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc
      601 tattggtgga tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc
      661 taaaccactt ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt
      721 tacgccatta aatggatacc aattaggatt aggctctctt tttttctgta catctcccat
      781 agaatagtta gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa
      841 taagatattt cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc
      901 catgctacct gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac
      961 aatcctttcc ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc
     1021 tggtcttggt ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg
     1081 aaacacagct ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat
     1141 tattcgcaca tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa
     1201 atatctactg gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca
     1261 aatcatcagc ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct
     1321 aatcaccaaa attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt
     1381 attgtgtcaa aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt
     1441 ctcctaatat aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat
     1501 gtggttttaa tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac
     1561 taaaatagat tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac
     1621 ttctccatct tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag
     1681 ctatgacagt atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg
     1741 gaaattctcc ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag
     1801 gaaatattaa gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa
     1861 tccaaaatat aatttgtaca attctcattg atccattgta ttccatttta ttccccctat
     1921 tactattcat atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac
     1981 cttgattttt tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt
     2041 gtctatctaa catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat
     2101 gcatctccct cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca
     2161 taagcagata aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact
     2221 gaatttgctg cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga
     2281 tttacgtgga cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga
     2341 actacgcaac aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg
     2401 aatataaaaa ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca
     2461 ttatactgta tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt
     2521 atcaatctat tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc
     2581 ctatgaatct cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta
     2641 attttaccca ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga
     2701 tgatcatttt aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat
     2761 cgcatctagc ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt
     2821 tcggtctcag aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg
     2881 ttgccgatga ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt
     2941 gcagatacca atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct
     3001 taccattaga tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta
     3061 ttttatgaat tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc
     3121 tgataaacca ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt
     3181 ccgagaacga aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg
     3241 gacttagtgt gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta
     3301 aggctatact gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta
     3361 ataatatgaa aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat
     3421 ttttatacaa tgaccattta tttataggct tttctttcat tttacttccc cctactgttc
     3481 tgttcacacg gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac
     3541 ttgctaacta tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta
     3601 ttcatctcat tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa
     3661 gaattattta gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct
     3721 atgatgaaaa gccttggaat acagtagctc ttaattggta atactttttc cattataaat
     3781 aacctctttt cattttatat ttatcccctc taaaaataat atctaatcta atattt
//
LOCUS       DAZKCE010000166         3818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000166 DAZKCE010000000
VERSION     DAZKCE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.171"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..309
                     /locus_tag="ACG8KQ_002753"
     CDS             <1..309
                     /locus_tag="ACG8KQ_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105638.1"
                     /translation="GKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVING
                     IFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL
                     "
     gene            complement(435..1112)
                     /gene="nth"
                     /locus_tag="ACG8KQ_002754"
     CDS             complement(435..1112)
                     /gene="nth"
                     /locus_tag="ACG8KQ_002754"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9105639.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1119..1817)
                     /locus_tag="ACG8KQ_002755"
     CDS             complement(1119..1817)
                     /locus_tag="ACG8KQ_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9105640.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2085..3269
                     /locus_tag="ACG8KQ_002756"
     CDS             2085..3269
                     /locus_tag="ACG8KQ_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9105641.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3275..3718)
                     /locus_tag="ACG8KQ_002757"
     CDS             complement(3275..3718)
                     /locus_tag="ACG8KQ_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9105642.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          993 a          754 c          634 g         1437 t
ORIGIN      
        1 ggaaaattta tggaaagaag ctctcatgca atgattctca cttttttatg gttgttgttc
       61 accagaatct atatttttct gatgagcctg aaaccgccca aatccattag tttatgttta
      121 gttataaatg gtattttttt attatcactt atcattttta ttacaccttt agatagatac
      181 gtcacttatt tatataatcc tttggaatac tggacttatt ttataggtat attggaaggc
      241 attattttct acatcgggta ttttcccaat aaaaataaca acttttattt ttatcgcaat
      301 aaattgtaat aagttaccat cctagctact gagtatgtac atttactttt tcatgcaaaa
      361 aagataaacg aaacagcttt tctccataat aacaagataa ttcatacatt tatttgcaat
      421 taaagaaacc acgtctacag gcgtggtttc ttttttagcg ccttttcttt tagacgcatc
      481 cgatttttcc catcttgaca tattgatagc aaaggacata cctcgcactt gggatttctt
      541 gccgtgcaat gatatctacc aaaaaaaatc agtgtatgat gtgtctttac ccataattct
      601 tgtggtactt tccgcatcag tgtttcttct acttccatga ccgtagcatc tagtttgcag
      661 atcctcaggc gttttgagac acgttccacg tgggtatcta ctgcgattgc tggtatcccg
      721 aatgcatccc ccaatacgac atttgctgtc tttctgccta caccaggaag agacatcaat
      781 tcttctcttg acgttggcac ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct
      841 ttgatgtttt ttgctttatt tcgatacaaa ccaatcgttt ttattttaag aatgatttca
      901 tcgattgaag cttctgctaa cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt
      961 gctttattca ctgatacatc tgtcgcctgg gcacttaaga tcactgcaat caataactca
     1021 aatggattat tgtgcttgag ttctccatgt gcttcgggaa acattccata catcgtttct
     1081 agagcttcca ttgttttttg tttgcttaac atattccgct actcctccaa ccaatttttc
     1141 agtgagactt tcggcaattc ttcttgttga gctgactgtt ctacttctct ttgaagaagc
     1201 tgctgttttc ttcgtttttg gtcttcttcc acttgttgtt ttgtacgtag attttttctt
     1261 tcccacgata gcaaaatgcg gtcaacgtaa ttaaaactat aagcctgatt taatacggct
     1321 tcccgcagtg ctagcttcaa aatttccggc tgataatggt cttcttctag ccattgtccg
     1381 atccgctgga actcgatcga agaaagcggg cgtccaaatt cttgttcaaa taattgatac
     1441 actgactgga tttctttttc ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa
     1501 tactcaccta atagatcata gatggggtaa agattatatc gatctgcttt tttaccgttg
     1561 acaatcgtcg tttctatctt gataaatcca tttgttacga gacgatccag tatttgaaaa
     1621 atctgatctt gtttgattcc catatccata gcaatcatct gcaaatcagg gaaagtatct
     1681 ccttcaagct gtgccatatg caactgtaga acaaataaaa attcttcaga ttgtagaccg
     1741 atcctatgat aatttttaag taaaagatta gatatgactg tttgtccagc gcttaagtaa
     1801 ttttctaaat tcaacatgat ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg
     1861 ggaagcaaga acaggtttct tttctattta cttcactgat cggtcagatt tacaatgtcg
     1921 tctacttgat ttttgtttgt tcatgttttt gatgcgtatc ttcacttttc ggaataaata
     1981 aattaagtag atttcgctgt tccttagaat gttgacaagg aaacaaaatt actatacact
     2041 atttttgttt ccaagttaac attattgcat aaaggagttt ttacatgtac agtttcacaa
     2101 ataaacattc taaagttttt ggatacgtct ctgtcttatt cactggatta ggatttacta
     2161 tcgtcagtcc tgtacttccg tttttaacgc tgccttattc tcactccata catcagcaag
     2221 ctttctatat cactttgctc atgtctgttt atgctctagc tgccttctta tctgccccca
     2281 ttctcggtag cttaagtgat cattttgggc gtcatccgat ccttattatc agtttattag
     2341 gatcaagtat cggatatttg ctttttggtt taggaaattc gatatggatg ttgttccttg
     2401 gtcgtatcat tgaaggattg acggcaggag aaatctccac cttatatgct tactttgccg
     2461 acatcacgga acctaatgaa agaaccaaag tttttggatg gatgggtgct ttagtcggta
     2521 taggaacaac gctgggtcct attatagggg gtctattagc tgagttgggg aattctgttc
     2581 ctatatttat tggagcactc tttactttct taaatgccgt ttatggctac acatttatgc
     2641 tagaaagcct tccaataaaa aaacgttcag tagatttaag tttctcccat gtaagacctt
     2701 ttcaccaatt acaacaatta ttcaagattt catccgttat tcctttattg acagcaggtt
     2761 ttgctgtttg gttggcagct ggctctcttc aatctatctt ttctcaattt tcgattgacg
     2821 cctttcagtg gaaggctggg ttggttggtt taagcttctc tttgattgga atcttggata
     2881 ctgtctcgca attgttcatt atgcctcgat tgctcaaaaa attttcagaa cagcagatca
     2941 caagaatagg gatgttcagt gaaatcctcg cctatctatt catcacatta tctggaattc
     3001 ttctactgcc gattttgttt ttatttggga ttatctgtta tggtttcggt gattctattt
     3061 ttactcctgt attcaatggc caactatcca attctgtctc agataaccag caaggtcttg
     3121 taatgggcgg tacccaaagt atacaatctc tatcgcgcgt catcggcccg ctaatcgcag
     3181 gacagcttta tgctgcagca ccatttcttc cgacggtatt cggatttttg cttatgattt
     3241 ttgcctatta tcactacaat aaaaaatagt agcttcattt gctacttcgt ataaattctc
     3301 tgtatcgacc gataaaacga aatactgtgt caaattcttc ttttgacata ttttcaaata
     3361 cagtagcatc tcgctgtacc catttttgat ggagttgctc gtgtctattc cttatttttt
     3421 gtccgattgg cgtcaattga aaatagattt cttttttatt ctcttcgctt tgataagtgt
     3481 ttatgatccc tctagctatc agtttttttg ttagcttact gatggctcct ctagtcatgt
     3541 aactagcatt cgctaatttt gtcacgttag gatcagggat tttcccgata tgttcaatca
     3601 cttctagttc atttagagta aatcctgcaa attctttttc catttctggt ttattacgat
     3661 gaataatctc attagctaaa tccagatatt cttctattag cagttgtctt ttatccattt
     3721 ctacaccttc tttttctttc attatattgt ttttttgttt cctattcaac aaagataaaa
     3781 atattttgta ggaaaaccca atctgaaacc aagaaaaa
//
LOCUS       DAZKCE010000167         3663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000167 DAZKCE010000000
VERSION     DAZKCE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.172"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACG8KQ_002758"
     CDS             <1..276
                     /locus_tag="ACG8KQ_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFP9105643.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACG8KQ_002759"
     CDS             419..640
                     /locus_tag="ACG8KQ_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105644.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACG8KQ_002760"
     CDS             656..1498
                     /locus_tag="ACG8KQ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105645.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACG8KQ_002761"
     CDS             1561..3363
                     /locus_tag="ACG8KQ_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFP9105646.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="ACG8KQ_002762"
     CDS             complement(3422..>3663)
                     /locus_tag="ACG8KQ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9105647.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAZKCE010000168         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000168 DAZKCE010000000
VERSION     DAZKCE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.173"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8KQ_002763"
     CDS             <1..186
                     /locus_tag="ACG8KQ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9105648.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8KQ_002764"
     CDS             329..553
                     /locus_tag="ACG8KQ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105649.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8KQ_002765"
     CDS             568..1437
                     /locus_tag="ACG8KQ_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9105650.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8KQ_002766"
     CDS             1418..2152
                     /locus_tag="ACG8KQ_002766"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9105651.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8KQ_002767"
     CDS             2185..3093
                     /locus_tag="ACG8KQ_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9105652.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8KQ_002768"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8KQ_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKCE010000169         3541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000169 DAZKCE010000000
VERSION     DAZKCE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.174"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACG8KQ_002769"
     CDS             complement(267..971)
                     /locus_tag="ACG8KQ_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105653.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1158..1403)
                     /locus_tag="ACG8KQ_002770"
     CDS             complement(1158..1403)
                     /locus_tag="ACG8KQ_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105654.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1420..2271)
                     /locus_tag="ACG8KQ_002771"
     CDS             complement(1420..2271)
                     /locus_tag="ACG8KQ_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9105655.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2437..3441
                     /locus_tag="ACG8KQ_002772"
     CDS             2437..3441
                     /locus_tag="ACG8KQ_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9105656.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          604 c          687 g         1152 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1141 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1201 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1261 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1321 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1381 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1441 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1501 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1561 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1621 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1681 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1741 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1801 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1861 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     1921 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     1981 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2041 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2101 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2161 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2221 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2281 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2341 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2401 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2461 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2521 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2581 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2641 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2701 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2761 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2821 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     2881 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     2941 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3001 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3061 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3121 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3181 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3241 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3301 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3361 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3421 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3481 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt
     3541 g
//
LOCUS       DAZKCE010000170         3502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000170 DAZKCE010000000
VERSION     DAZKCE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.175"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2244)
                     /locus_tag="ACG8KQ_002773"
     CDS             complement(<1..2244)
                     /locus_tag="ACG8KQ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9105657.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTG"
     gene            complement(2916..3184)
                     /locus_tag="ACG8KQ_002774"
                     /pseudo
     CDS             complement(2916..3184)
                     /locus_tag="ACG8KQ_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          985 a          774 c          552 g         1191 t
ORIGIN      
        1 tcctgtagtt agaccggttt gtagcgtttc tccttctcgg ttctgtaact caaaaacagc
       61 tccttgaagg acttcaccac tgccttcagc atgtttaatt agttcaacac cacccgtatc
      121 aaggatgttt tcaatggtta acgtcacttc ttctatggag tattcattta taaaaacatc
      181 atgtaccgtc gcatctaaaa gatacccttc cggtgcttct gtttctcgga cttgatagct
      241 acctacagga agcggttcac ttaaaaattc tccgttttct gccgttatac cttcttgtat
      301 cacttgtgaa tcgcttgata aaatttcaaa ttttgctcct gccagccttt ggccattctc
      361 atcagtttta attactttaa tacgcccatc gattttcctt gttcttacag tactcgtatt
      421 caatcgattg tcactattca caagcgttgc aaagtttaaa atttgatcgc cgaccgcata
      481 cgcctctaca ttgaccttaa aagagagggt aaaacttgtc ggaccaccaa tcgtattatt
      541 cgcactgaaa cgaactattc cattattgac ttcggcatca atgaaagagg gaatatctga
      601 tagaaatgac acaaattcca tgccttcttc caaaacatct tcaataacaa gcgtatttct
      661 tgttccagaa aacatcgaga catgcagagt atacgttagt tcattttcat tgagattgat
      721 aacatttttg tccacacttt ttcttaaaga gattgccgga agattacttt cagggattct
      781 caagcggtag tctcggctca ttacctgatt attgtaggtc atactcgctc gattccgaga
      841 aaacccatcc tcttgtcttc cttcattgaa caagtcgaca tcggtcactt caagagcata
      901 ttcaataaaa tacgattgac cctctgctag attaccaaag tccacaacaa gtgtattcgt
      961 ttccttatta aaagatactt tgtccacaaa ttcctcggaa acataatgag ttctaacgtt
     1021 tgcttcacct ggattcgtta cttctgcgat ccgaacccca cctgaaatag gttctctatt
     1081 tccagtggaa ctccatgtag tcggattcgg tgccaaagtt gttccttgcg gtagtacatc
     1141 ggtaaccact acaccgtctt ttcgtgatcc ttcataattt acctgcaaaa taaatttatt
     1201 actgttggtc acgtcttgcc cgtcaatcag cagattccca agatttcctt ggctgtcgat
     1261 ttctctagag ccatagtata atttcgaaaa aatgggtctt gatgaaggaa taggcgtaat
     1321 tgaaaattcc gtgtttgctg ctcgctcagc ataagtaaga ctcacttgaa agtcattatt
     1381 tggctcagta ccgcctgatt gaaagaaagc aacttcaaac gtgatactga atgtatacga
     1441 ctgagcggct tcatttaatc cttcataatc aaaggtaaat ggaaaaacaa cgtctgtgcc
     1501 ttcttggtaa aatgccagtg gctttaaagg tgaatcatca gcgacctcca ctctaatgaa
     1561 ctcaattaag ccaatcgatg ctctaggaac tcgtacgacc atctccccat attcttcaag
     1621 aatatcgcca gcgcctcctt gaatgtatcc ctttaacgtt tgtgtatgcc tgctcggcaa
     1681 agtggggcgc tcagcagaga tgccgatgtc ataattcaag agctcctctt gtctttcaat
     1741 ctgagattcc tgcgttatag atggctcaga tgatctggct tcattaaaag attcacttaa
     1801 gctttcttct ggtatattaa aagattcact tgtgctttct tctggtacat taaaagactc
     1861 acttgtgctt tcttctggta cactgatttc tattgagacc gtatttgatt gaatatcacc
     1921 gcttcttgcg gtcaattcgt atttacctga atgctgggaa gaataagtaa tcagacgttc
     1981 gtaatcattg agttgcttca cttccccttc aatcaaatcc tcactcttat caatgaccaa
     2041 tgcatttagc aggtctggtg ttcctctgac tcgcaactgg aactgctcac ccaaagcagc
     2101 ctcctcttga tcaacttcga tcgtcagcga acgctctggc tgttctgcta aagactcagc
     2161 tagaaccgtt atgttcccaa attgattgat cacaataagc ataactagaa acatacttaa
     2221 cagtttatga aatttgttcc tcactattac tcccccttat aattttgact aaacaacaag
     2281 gtacccgata attttctaaa aagattcccc aactcaaaat ggctgcgtta cctccctcgt
     2341 attttcggtt caactcaagc gttgcaatgt aagctgtaaa caaagctaat gattgccgat
     2401 tgtcttctgt tgattgattc gatagctttg tatgactttt agttgtccgt gtggcttaac
     2461 cataattatg atttcacgat tcctcctgcc agtgccttgc acaagctgga gcgtaaccct
     2521 aatagttatt ttgttaatgt acttatatac ttttatactt ttccattaga aacaatcact
     2581 aaatcgttga taccaaggtc agactctaga atttcatcag tgagtccctg tattggttaa
     2641 ccttcatcaa tcccaaaaaa atatatcaat gcttagcatc aaaatgaata tatagtttag
     2701 aatacttggg attcatcgat aactgatcgc ttatttggcc atattatttg gcacttggtt
     2761 caactaaacg catttaggat gttccttcat tctcgtcatg gatgttctca aaccgtcatt
     2821 actaataaac tgtccaccga atcgatggtt catttcttgc attttccacg tccaattact
     2881 ctttttgctc tttatttggg cgtagtttca ctttcttaac ttgtatcgtc ttccatcaac
     2941 tccttaatca tcttcttagc tttattggct cggtttcctt gtaaaaggta tctacaaata
     3001 gtgacaatct gtattaaatc ttcatcattt tctgtaatgt caataatttc tatattctgt
     3061 accatatatt gcaaagggtt tcactaagtt cataaccgaa gcgaattaaa caacttttgt
     3121 atagaataac taccttttct atttttccat tggtaattct atctattaat tggtttaatc
     3181 cttttgttgt agttgattcc actaccaaga tgattctctc gaattttaaa gcttgaagat
     3241 gtattgctat ttttgctaac tggcgtattt gcaactattt ttcccttcta ttcttccttc
     3301 attgattccc ctctaaaatt actatccata aaaaattgca taaatgacaa tagtatttat
     3361 attagtcata ctgcttttta acacagcaaa ttccagaaaa acattcgtta catatttgtg
     3421 aatttttcca aaaaataaca tttttataaa acgacgaaaa ataaatcact accagtcaac
     3481 ttctataata ttaccaaata ac
//
LOCUS       DAZKCE010000171         3321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000171 DAZKCE010000000
VERSION     DAZKCE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.176"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            717..1049
                     /gene="traE"
                     /locus_tag="ACG8KQ_002775"
     CDS             717..1049
                     /gene="traE"
                     /locus_tag="ACG8KQ_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9105658.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1668..2009
                     /locus_tag="ACG8KQ_002776"
     CDS             1668..2009
                     /locus_tag="ACG8KQ_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9105659.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2246..>3321)
                     /locus_tag="ACG8KQ_002777"
     CDS             complement(2246..>3321)
                     /locus_tag="ACG8KQ_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9105660.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1079 a          490 c          676 g         1076 t
ORIGIN      
        1 agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt acgtcataag
       61 tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg catactcctt
      121 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
      181 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
      241 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ttgcactttt ggatgataaa
      301 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
      361 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
      421 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
      481 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcataatgt
      541 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
      601 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
      661 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
      721 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
      781 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
      841 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
      901 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
      961 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
     1021 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
     1081 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     1141 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     1201 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     1261 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     1321 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     1381 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     1441 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     1501 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     1561 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaatta
     1621 atataaagca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     1681 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     1741 aaggatttgt gacgtttaga aggggcggaa attgtaaagg attagatgtt ttagcattag
     1801 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     1861 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     1921 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     1981 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     2041 cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
     2101 atttatttag tctaatggtg ataagtcaag atgaaattta agattttctt tattgagtaa
     2161 tatgttttga agcgcacgaa actagacccg atcaaaatca aatttttttc gggttgattc
     2221 aaggatttgc gtcaataacg gattgttatt tctctatcac actccttggt ggcgtataga
     2281 gttggtgttt acgtagtagc gtatccacca gacgcgtaaa ttttcttgcg gttaggacga
     2341 gtgcacgttt gtgttgatgc tttggaacct ccttatattt actttgataa aaagctttgt
     2401 attcaggaag ataatttttt acagagttgg cagcttcaac taagtagtat ctaaaatatc
     2461 gatttccttg ttttgtcaga ggtgtatttt gcgactgata gtttccagat tggttcactt
     2521 tccaacttaa tccagcgtat tttgccaatt ttgtctgatc ttcaaatctt tcgatttgac
     2581 caatttcagc gattaaaccg gcagcataga ctttaccaac gcctggaata ctggttaagc
     2641 attgatattc tggaatgaca acaattactt gttcgatggc tttatctaat tctttgatat
     2701 ttttttcaag tgctcgtatt tctcgtacga gaacacttaa aacaacatta attgattctt
     2761 gtgcaagagc acctaggcgg taactcatgg taattgcacg ttgaattgct ttagcgattt
     2821 tttctggttg cttgaaacgg ccttttcctt tttcttgaag taaatcacag aaagcttcta
     2881 aaggaagctc agcaagttca tccaatgtga aatcttccgt catgagtgaa atgagggttg
     2941 cactaaaaag actcgtcgat tcgtctcgca tttctcgagc aagcgtatta catttgtacg
     3001 ttagattttc aagaaaatgt tgctttgtac gaatcaattg cttaataagt tgatagcgag
     3061 tacgtgttaa acgctgtaag gccatgtatt tctcttcttt gataggggaa gttgtgaatc
     3121 gttgaatacg caagaaatca gcgattctta atgcatcgtt tcgatcagtt ttattctcat
     3181 caaacatgcg actaaattgt ttcacccgaa aaggattttc aatcgttaca agtgtgttga
     3241 gcttctttag tttctcatct tcatgaaaaa acatggaagg atggaagctg tacatggacg
     3301 ttgactccat gccaatcaca a
//
LOCUS       DAZKCE010000172         3273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000172 DAZKCE010000000
VERSION     DAZKCE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.177"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2165)
                     /locus_tag="ACG8KQ_002778"
     CDS             complement(552..2165)
                     /locus_tag="ACG8KQ_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9105661.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ACG8KQ_002779"
     CDS             complement(2306..3214)
                     /locus_tag="ACG8KQ_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105662.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1058 a          560 c          573 g         1082 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caa
//
LOCUS       DAZKCE010000173         3246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000173 DAZKCE010000000
VERSION     DAZKCE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.178"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            816..1202
                     /locus_tag="ACG8KQ_002780"
     CDS             816..1202
                     /locus_tag="ACG8KQ_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9105663.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1296..1487
                     /locus_tag="ACG8KQ_002781"
     CDS             1296..1487
                     /locus_tag="ACG8KQ_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105664.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2001..2333
                     /locus_tag="ACG8KQ_002782"
     CDS             2001..2333
                     /locus_tag="ACG8KQ_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9105665.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2243..2437
                     /locus_tag="ACG8KQ_002783"
     CDS             2243..2437
                     /locus_tag="ACG8KQ_002783"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9105666.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2476..2622
                     /locus_tag="ACG8KQ_002784"
     CDS             2476..2622
                     /locus_tag="ACG8KQ_002784"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105667.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            3060..>3246
                     /locus_tag="ACG8KQ_002785"
     CDS             3060..>3246
                     /locus_tag="ACG8KQ_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9105668.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT         1175 a          538 c          507 g         1026 t
ORIGIN      
        1 aaataagtag ttctatctga gcgatactct tttataatac tgaattcgga attaaaaacc
       61 acgataggta actttaaaca aacatgaaga gactttaata agacttcttt atctgaaaac
      121 acttttgttt ctctctttcc tttctacatc attaaatagt attttatacg ttatctaaaa
      181 tactattcct ctgtcaaaat aagctaactt acttcattat aaaatatcca cttatcgctt
      241 tcatgtatat tccaccgtaa aaagtacatt ttatcaccga gaactcatca tttttcatca
      301 tgatgaatac tatttacata tatctagttc aaagacaata attattatga tccgttatta
      361 tctaagcact atacagataa taggaatttt tcgtttctaa atattccaat ttgggaatcc
      421 aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg ttaattatga
      481 agggggaaga aataaaaaat acaatctata cacgaataag aatattatcc aaaaggaggt
      541 tttagaaaag atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg
      601 tttgatattt aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta
      661 tcaactaaaa aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt
      721 atagaagtta ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct
      781 tttcccttta aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa
      841 atgcccagtt gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata
      901 aaaaacaaga agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta
      961 ctaaagatac tataaaattt aaacaaccga ttaatgtttt aaatgtgact ctccttgttg
     1021 aagatataaa attagcaaaa atccttcctt tgttgaaaga acctgaacaa atttttctta
     1081 tcgaaaaatt tatctttgat aaaactgata aagaaattgg aaaggttctg gggattacaa
     1141 gacagggtgc gacaaattta aaacatcgac tttacaaaaa aattcaaaaa atgctggttt
     1201 aatatctgca taataactaa ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga
     1261 aatagattat aagtccatgg gaagaaggta gatttatgaa ttctttaaaa gaagatttca
     1321 ttttagcaaa ggctggaaat gaagaagctg ttgaggcaat tctcaaaaga tttagctctt
     1381 tgattcataa gcaatcctgg agaactggaa aatatgatca agattgttat caagaatgta
     1441 tgcttgcaat atatctagct atctcgaaat tcgaaattaa agaataactc aactaaggga
     1501 gtctgtgaca taagtaaaaa gatagtttca aatttcgaac aataaatttg aaactatctt
     1561 ctttataatt ataaagtatc gattttacag cgttctataa tgattcatag aatctaaaac
     1621 gaatgatttt aattatcaac gttttttaat tcgtttttag cttaaaaata acttttgtca
     1681 cagtctcact cctaaaatta ctttatttac gtatgaagtg ggcaggaggt cgaatccaga
     1741 actgggctat ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac
     1801 cttccgtaac gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca
     1861 aaaatagtca agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag
     1921 ttgacaaacc ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt
     1981 accggaagga atgaactcta ttgagaaaaa catatgatcg taaatttaaa ctaaagattt
     2041 cacaggatat ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct
     2101 ctcgtccaac aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg
     2161 ctggacaagg aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata
     2221 aacgactaaa agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg
     2281 gatcactcaa aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct
     2341 tatgcgtgaa atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag
     2401 agaaaaaaca atcaaagaaa tggtaaaaaa atgttgaatc gataatttga acagaaccaa
     2461 attgatgctg tttgggtgat ggatattact tacattccat gtaatgacgg tcgcctctat
     2521 ttgtccacct atattgactt agcaactcgt attctacgtt actatatggt agattcacac
     2581 atgaaaaaag agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta
     2641 attcattccg atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaacgac
     2701 caccacatca cacattcagt gagccaaact gggactcctg ttgacaatgc agtaattgaa
     2761 gcgtttcatc gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata
     2821 gagatgaagg tgttaatcca agattattta gaagactact acccaaacaa aagaatccat
     2881 acaaaattta tgatgactcc ttgacaattt gaagcgaaga aaataaggga tctataatat
     2941 ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca
     3001 gacaccttaa aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga
     3061 tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat cttatctttc
     3121 atgaagatgc ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca
     3181 cattgaccta tcaaccggag gaatgctttc atcgccacta tcaaaatgaa caaacgatca
     3241 ttaaat
//
LOCUS       DAZKCE010000174         3201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000174 DAZKCE010000000
VERSION     DAZKCE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.179"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8KQ_002786"
     CDS             301..585
                     /locus_tag="ACG8KQ_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105669.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8KQ_002787"
     CDS             588..1004
                     /locus_tag="ACG8KQ_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105670.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8KQ_002788"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8KQ_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9105671.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2523..2609
                     /locus_tag="ACG8KQ_002789"
     CDS             2523..2609
                     /locus_tag="ACG8KQ_002789"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9105672.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..>3201)
                     /locus_tag="ACG8KQ_002790"
     CDS             complement(2912..>3201)
                     /locus_tag="ACG8KQ_002790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105673.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DAZKCE010000175         3100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000175 DAZKCE010000000
VERSION     DAZKCE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.180"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="ACG8KQ_002791"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="ACG8KQ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9105674.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="ACG8KQ_002792"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="ACG8KQ_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1462..1701
                     /locus_tag="ACG8KQ_002793"
     CDS             1462..1701
                     /locus_tag="ACG8KQ_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9105675.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            1765..1818
                     /locus_tag="ACG8KQ_002794"
     CDS             1765..1818
                     /locus_tag="ACG8KQ_002794"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9105676.1"
                     /translation="MRNVDKTSTVLKQKSSP"
     gene            complement(1856..3028)
                     /locus_tag="ACG8KQ_002795"
     CDS             complement(1856..3028)
                     /locus_tag="ACG8KQ_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP9105677.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT         1059 a          543 c          561 g          937 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atatctttct
     1321 caaaataaac ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat
     1381 cacaaatcac ttatcacaaa tcacaagtga tttgtgattg ttgatgataa aataagaata
     1441 agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga
     1501 aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt
     1561 gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
     1621 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa
     1681 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
     1741 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
     1801 cagaagtcaa gtccataatc ctgtgtaaaa tactatacaa tgttttaccg gtctctcatt
     1861 gatcaaactt aatatatttt cttgtagaac tgagccattc gtcatgaagg tccataagga
     1921 cagctccaat taatcgattg gcagacgttc ggttgggaaa aatccgaata atcttttctc
     1981 ttctgcggac ttcctggttc agtcgttcaa gaaggttggt gctttttagc cgattatgac
     2041 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
     2101 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
     2161 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
     2221 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
     2281 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
     2341 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
     2401 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
     2461 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
     2521 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
     2581 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
     2641 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
     2701 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
     2761 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
     2821 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
     2881 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
     2941 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
     3001 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     3061 aaaacattgt accgtaaaag ggctgttaca tggcctttta
//
LOCUS       DAZKCE010000176         3087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000176 DAZKCE010000000
VERSION     DAZKCE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.181"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(350..712)
                     /locus_tag="ACG8KQ_002796"
     CDS             complement(350..712)
                     /locus_tag="ACG8KQ_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105678.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(786..1166)
                     /locus_tag="ACG8KQ_002797"
     CDS             complement(786..1166)
                     /locus_tag="ACG8KQ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9105679.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1248..2423
                     /locus_tag="ACG8KQ_002798"
     CDS             1248..2423
                     /locus_tag="ACG8KQ_002798"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9105680.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2500..3084)
                     /locus_tag="ACG8KQ_002799"
     CDS             complement(2500..3084)
                     /locus_tag="ACG8KQ_002799"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9105681.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          960 a          589 c          507 g         1031 t
ORIGIN      
        1 caagtggttc ttttttaacg actcagttat cttatcatgt cgttagtaac atgtcaataa
       61 ctatttttta aattttttag aaatgtgata taacatccac ttcagaatca acgctgttaa
      121 atttatcatg tatttcttcc ttggtcaact ataaattaag cattactttg tgaaatgatt
      181 tttcgatcaa ttattattat tcgttataca accgttttca acatattttg aaaataaaat
      241 gcactaaaaa actatcttta gcagaacatt tcattgtatt ttttggacca aacaataaaa
      301 aaagaagttt gaaaaaaatt caaacttctt tgttcgctta atctgtattt tagaagaaag
      361 gcactccctc tacttcgtaa gttccaagaa cagagcgcat ttgacctggt tcttcccaaa
      421 tatatccttc gcctaattct tctctttgat tcttttgttc agtttcaaag acaaacaacg
      481 gaacattttc ctctttaata tgtgtatttg tcaattccat caaactaatc ttgctaacat
      541 cgatatgaac tatttctttt aagaaattaa gtatattcgc taaaccagtc tttccttcag
      601 aaagttccgc taacgcaaat tccaacttgt catcaattga atggaccaaa aattttttag
      661 taccatcctc caaatgcaac ataattgtac cagcaactct tttgcttgcc aaaatttcca
      721 cctcgatcaa cttgttctat ggcttacata aaaattctag cacattttca gaaaaaatac
      781 actttttatt tcttattttc tttccgttct agttcagata atgcttcttc tatccaaata
      841 ggtaactgct ccgcaatagt ggtaaatccg atcttacgat ttttattcgt gaaatacctt
      901 tttctgcgat atggaacagc tggatttgtt ttttctaacg ttcttaaaga taaactgatt
      961 tttttggaat attcatcaat gtcgatgatc tgaactgtta ctggctgtcc gacttttaac
     1021 aatgaatgga tatttttcgt ataaccggat tgtacttctg aaacatgaat aagtccttgc
     1081 gtctcgttat caagagaaac gaatgcgcca taaggctgta tccctgtcac ttttccttca
     1141 attatttgtc caattttata agtcatctac ttccctctct tcgcatttat cattatactg
     1201 taaatagctc aaaaaaagaa gcgacgaaaa tgactggagg tcttaagatg atcgaatttg
     1261 ataaacagat ttcaagaaaa gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat
     1321 atcaaatgga tgacttactt cctttgtggg tagcagatat ggacttcttg tctcccggtg
     1381 gggtagctaa agcgtttagt gaatacatca aacatggaat ctttggttat gccactctgt
     1441 ctgataaact atatcaggct atcattcatt gggaacgcca acaccatgct gtggagttga
     1501 cgaaagaaaa tatcgttttt acaagtggtg tgttaaccag tttagctgca gcagtacaaa
     1561 cttttacaaa accaggcgat tctattttga ttcatgaccc agtttatcct ccattttcct
     1621 ctattataga aacaaatcaa cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc
     1681 atttcatcat ggacttgtct gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa
     1741 tgattttatg caatcctcac aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac
     1801 agttaagcga attgtgttta aaacatcaag tgattctgtt tagtgacgag atccatcaag
     1861 atttaacttt attcgatcat acatttactt ccatgctaac gattgatcct aatttagatg
     1921 atctactagt tgcttttact tccgcaacca agactttcaa tctagctgca atcaaaaatt
     1981 caatggtatt tatcaaaaat ccagagatga aagctgattt tgaaaaacat ttagtgatga
     2041 accaacagca cgaaatcaac acttttggac tgattggaac ccaagcggct tatgagacgg
     2101 gtggagaatg gttaagccaa ctgattcctt atctagaaaa aaatgttcaa acagttaatc
     2161 agttttttga acagtatctt ccgaaagtga gaataatgaa ccctgaagga acttacttaa
     2221 tgtggttaga ttttaccgcc tatatttcag acgaccaaga attggagaac actcttgttc
     2281 ggaaaggaaa agtcgtatta aatcctggca tcacgtttgg tccttccggt catgggcata
     2341 tgcgcttgaa tattgcttgt ccagaagaaa ctttactgga aggacttaat cgaatcaagt
     2401 atgctttaga gacaatcgat taaatcattt attcaaaata aaaggacgag accttactag
     2461 gtttcgtcct ttttcataat tactccgtac cataccaaac tattctgaaa tttcaattgt
     2521 ttcaatcact acatcatgta caggtcggtc ttgtggacca cgttgtacat ttgcgatttc
     2581 atctactgtg tccatccctt ctagtacatg tccaaagact gtatgtcgga aatctagcca
     2641 aggagttccg ccacctttat acgcttcaat gatttctgat gggaatccag caccttctag
     2701 ttgtccaagc atattagctg gcacatttgt gttatttaca ataaagaatt ggcttccgtt
     2761 tgtgtttgga cctgaatttg ccatagataa agctccgcga aggttgaaca gttcacgact
     2821 gaattcatct tcaaaacttt cgccataaat actttcgcca cccattcctg ttccagttgg
     2881 atcgccgcct tggatcatga aatcaggaat aacgcggtga aaaatcacgc catcataata
     2941 accttttttg gaaagttcaa tgaaattttg aaccgtcttc ggtgctagtt cagggaataa
     3001 ttgaacagta atgtcaccac ggtttgtttt gataacagct tttggtcctt tttcactttt
     3061 taaatctagt tgtgggaatg ccatatt
//
LOCUS       DAZKCE010000177         3073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000177 DAZKCE010000000
VERSION     DAZKCE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.182"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1104
                     /locus_tag="ACG8KQ_002800"
     CDS             289..1104
                     /locus_tag="ACG8KQ_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105682.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="ACG8KQ_002801"
     CDS             1230..1487
                     /locus_tag="ACG8KQ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105683.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="ACG8KQ_002802"
     CDS             complement(1739..2311)
                     /locus_tag="ACG8KQ_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9105684.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8KQ_002803"
     CDS             complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8KQ_002803"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP9105685.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1020 a          539 c          473 g         1041 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tag
//
LOCUS       DAZKCE010000178         3073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000178 DAZKCE010000000
VERSION     DAZKCE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.183"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..959
                     /locus_tag="ACG8KQ_002804"
     CDS             192..959
                     /locus_tag="ACG8KQ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105686.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            949..2991
                     /locus_tag="ACG8KQ_002805"
     CDS             949..2991
                     /locus_tag="ACG8KQ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9105687.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          992 a          488 c          619 g          974 t
ORIGIN      
        1 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
       61 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
      121 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
      181 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
      241 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
      301 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
      361 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
      421 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
      481 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
      541 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
      601 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
      661 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
      721 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
      781 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
      841 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
      901 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
      961 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
     1021 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
     1081 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
     1141 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
     1201 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
     1261 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
     1321 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
     1381 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
     1441 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
     1501 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
     1561 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
     1621 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
     1681 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
     1741 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
     1801 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
     1861 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
     1921 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
     1981 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
     2041 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
     2101 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
     2161 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
     2221 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
     2281 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
     2341 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
     2401 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
     2461 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
     2521 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
     2581 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
     2641 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
     2701 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
     2761 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
     2821 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
     2881 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
     2941 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
     3001 taagacaaga taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg
     3061 tgacacgatt ttt
//
LOCUS       DAZKCE010000179         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000179 DAZKCE010000000
VERSION     DAZKCE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.184"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8KQ_002806"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8KQ_002806"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9105688.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8KQ_002807"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8KQ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9105689.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8KQ_002808"
     CDS             2067..2819
                     /locus_tag="ACG8KQ_002808"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9105690.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKCE010000180         3011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000180 DAZKCE010000000
VERSION     DAZKCE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.185"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            685..>3011
                     /gene="esp"
                     /locus_tag="ACG8KQ_002809"
     CDS             685..>3011
                     /gene="esp"
                     /locus_tag="ACG8KQ_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP9105691.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1102 a          453 c          582 g          874 t
ORIGIN      
        1 taactttttc agaaaagtta aacaactaca agttaataga ttataagtat ttgagtgaaa
       61 gcaattaatt attcatgtag agtactagac actttaaata tggacaattc aattataaag
      121 aaaaagaatc aataatttaa tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac
      181 gagtgaaaaa atgttgataa ctccaatcat tcgtttaaga ttctaggaat cattcagaaa
      241 tgctgtgaaa ccgatacttt ataattataa agaaggtgat ttcaaattcc gaagaaggaa
      301 tttgaagtca cctttttact tatgtcacag tctcattcag taatgcggga taaggctcct
      361 tattccgtta tttgaatact ctgaagttta atctaagaaa gggtaatatt ttgaaaacat
      421 gcttcatcgc atagaaatga acttaatttc agaaagaata catacatatc tttttatatg
      481 ttttcagctg ctgaatggtt aaaaagcaca aatatccgaa aaaaacatga agaaatgtac
      541 tttttttgtt gtaatgttca tgaaattctc taagtaaatt agttaacata aggttagtaa
      601 gattcctatt ctgtgaataa tacgtatcag atttatcttt tattcttact aggaaagtaa
      661 ttattaaggg agttgatttg aataatggtt agcaagaata ataagagagt atttctagaa
      721 aaaacaaaga aacgagtatt gaagtacagt atcaaaaaat taagtgttgg ggttgcatct
      781 gttttgattg gtgttggtct tgtcttggga acaactgaac tagttaaagc acaagatgaa
      841 atttctccaa gcagcccact tgaaacagcg ataagctccg tacaagtagg tgacaaagta
      901 gcttcgggga atacttttca ggagaatcct ggttatacga aaaattataa cttttcggat
      961 ttacaattta gtcctcaaga actaactgga gacacactaa aaggcaatac aattggattt
     1021 gaagtttacg gaaaacataa tattgcagca tcaactaaaa actgggaaat ccgtcttcaa
     1081 ttagatgaac gattagctaa atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt
     1141 ggatcttcta gacggacctt tgtaagaatt aatgattcgc tgggtagacc tacaaatatt
     1201 tggaaggtca attatattcg tgcaagtgat ggattatttg ctggggcaga aacaaccgat
     1261 acgcaaaccg ctcctaatgg ggtaattacg tttgaaaaga gcctagatga aatttttaaa
     1321 gagataggca tagacaatct caagaccgat cgtttaatgt atcgaatcta cttggtaagt
     1381 catcaagacg atgataaaat tgtaccgggt attgatagta cgggctactt tttaacagat
     1441 tcagatgatt tctataatag cttagatgta tctgaaaata atcctgacca gtttaaacat
     1501 ggatcagtaa gtgcaaaata tgagaaacct aatactcaaa caaaagatgg ttcaggatcc
     1561 acaggtgcta atggagctat aatcttagat cataagttaa cgaaaaatta taatttctca
     1621 tattcatctt ccgcaaaggg cacgccttgg tatgctaatt acaaaattga tgaacgatta
     1681 gttccatatg tagcgggtat acagatgcac atggttcagg ctgacaaagt aacatatgat
     1741 gtttcgtttg aatccggaac aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat
     1801 gaaaattatg gcataggttc aataactaat aatgatttaa ctaaacttat cgattttgct
     1861 aatgcaagtc cacgtccagt cgttattcga tatgttttac aattgactaa gccattagat
     1921 gaaatcttag aagatatgaa agcaacagca caagttgaag aaaataaacc atttggtgaa
     1981 gatttcatct ttgattcttg gttgtcggat acgaataaaa aattaatcca gaacacttat
     2041 ggaacaggtt attattattt gcaagatatt gatggtgatg gaaaccctga cgataaagaa
     2101 gagagcggag acacgaatcc atatatcggg aaacctgaat tagaagaagt atatgatgtt
     2161 gacacaacag ttaaggggaa agtattcatc cacgagttag cgggaacagg tcacaaagcc
     2221 caacttgttg ataaagaagg tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa
     2281 gatggggctc caatttcaga tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa
     2341 ttaatcgcga aagatgaatt aaaaatccaa atcgtttctc caggttttga taaaccagaa
     2401 gaaggttcaa ccgttattaa ggaatcacca aaagcggttg ataaacaaac cgtggtagtt
     2461 ggatttaaac cagatgctaa agaatcaatt cggaataata aaaacttacc tgaagatgca
     2521 gagtattcat ggaaaacaga gcctgatact tctaacgtta ctgatagtac gaaaggtatt
     2581 gtaactgtta agatcggaaa tcgaactttc gacgtggatg tagagtttgc tgtaaaagct
     2641 tctcaagcta tggaaaatga tgcaacatac gtacctataa caacaactcc agaaacgaca
     2701 gttcaaagtg gaaaacctac atttgataaa ccagatgttc ctttagctaa agatgccttt
     2761 tcaatttcag atgtctataa taagtacttc ggtaatgcaa gtgttgatgc aaacactggt
     2821 attgttacat tcactccagc taaaggtgta ggagaatcgg agccgattac tggaacaatt
     2881 cctattaaaa ttgtttacca agatggttct gtaggcacga ccgatttagc agtaactgta
     2941 agtaaagata tttatgaaaa tccaggagaa aacattcctg caggctacca caaagtaacc
     3001 ttcaccgcag g
//
LOCUS       DAZKCE010000181         2951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000181 DAZKCE010000000
VERSION     DAZKCE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.186"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..254)
                     /locus_tag="ACG8KQ_002810"
     CDS             complement(57..254)
                     /locus_tag="ACG8KQ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105692.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            423..1001
                     /locus_tag="ACG8KQ_002811"
     CDS             423..1001
                     /locus_tag="ACG8KQ_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9105693.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            1096..1233
                     /locus_tag="ACG8KQ_002812"
     CDS             1096..1233
                     /locus_tag="ACG8KQ_002812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105694.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(2046..2549)
                     /locus_tag="ACG8KQ_002813"
     CDS             complement(2046..2549)
                     /locus_tag="ACG8KQ_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9105695.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            2803..>2951
                     /locus_tag="ACG8KQ_002814"
     CDS             2803..>2951
                     /locus_tag="ACG8KQ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9105696.1"
                     /translation="MKNQEIVVILGNADKRDWHFILVQLFFQLLGRRDRLFKTYFRFN
                     KIQDNP"
BASE COUNT         1052 a          437 c          528 g          934 t
ORIGIN      
        1 tcgcgaccct atttttttag taagttatat cataatgaag tgaatggtat catacattaa
       61 taatagtttt ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg
      121 attgggatta gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga
      181 ctgatgacga agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata
      241 tttttttgac gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac
      301 agggattgtt taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga
      361 tcaaacaatt gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata
      421 tcatgcctaa aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta
      481 ttttagtttt agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag
      541 gttaccgatt ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc
      601 atcatcattt atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt
      661 tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca
      721 atagcctggc tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt
      781 atatcttggc aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga
      841 tgttgaataa ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct
      901 tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt
      961 ggatcatccc aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat
     1021 ttaagagaaa aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt
     1081 caaagaggga tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc
     1141 acgatgctta tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga
     1201 tttttgacat tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat
     1261 ctatttaaga aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt
     1321 ggaaaattta atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg
     1381 ataacttcgt aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt
     1441 gataggagat atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg
     1501 gtgtggtttt tggaataaaa aaataacata tctcatttta cacttttgtt attttatact
     1561 taaaaaataa cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta
     1621 ctgttttttt cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa
     1681 tcaaggagaa gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta
     1741 taaaacaagc tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat
     1801 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
     1861 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
     1921 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
     1981 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
     2041 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
     2101 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
     2161 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
     2221 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
     2281 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
     2341 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
     2401 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
     2461 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
     2521 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
     2581 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
     2641 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
     2701 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
     2761 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     2821 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     2881 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     2941 caagataatc c
//
LOCUS       DAZKCE010000182         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000182 DAZKCE010000000
VERSION     DAZKCE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.187"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8KQ_002815"
     CDS             85..981
                     /locus_tag="ACG8KQ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9105697.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8KQ_002816"
     CDS             1073..1288
                     /locus_tag="ACG8KQ_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9105698.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8KQ_002817"
     CDS             1306..1578
                     /locus_tag="ACG8KQ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9105699.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8KQ_002818"
     CDS             1580..2443
                     /locus_tag="ACG8KQ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9105700.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKCE010000183         2899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000183 DAZKCE010000000
VERSION     DAZKCE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.188"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..556
                     /locus_tag="ACG8KQ_002819"
     CDS             <1..556
                     /locus_tag="ACG8KQ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9105701.1"
                     /translation="FFDDKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVR
                     KKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGS
                     IVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVK
                     DIPSHSLAYGNPAKIKKEGINFEF"
     gene            507..1688
                     /locus_tag="ACG8KQ_002820"
     CDS             507..1688
                     /locus_tag="ACG8KQ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9105702.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1701..2678
                     /locus_tag="ACG8KQ_002821"
     CDS             1701..2678
                     /locus_tag="ACG8KQ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9105703.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            2678..>2899
                     /locus_tag="ACG8KQ_002822"
     CDS             2678..>2899
                     /locus_tag="ACG8KQ_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9105704.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLE"
BASE COUNT         1060 a          360 c          565 g          914 t
ORIGIN      
        1 attttttgat gataagcaaa ataaaatgca agatctgaaa tatcttggga aaataaacga
       61 attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag ctattggtga
      121 ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc aatatggaat
      181 agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg gaactgttat
      241 ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta taataaacac
      301 aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat ctccaggtgt
      361 atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg gttctcaatg
      421 tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca ctattgttaa
      481 ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga aagaaggaat
      541 taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca gaagaaaaat
      601 atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat gtggataaat
      661 ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg ttaagttcag
      721 gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac gatattgtct
      781 ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa ggggctgtac
      841 cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca ctagagagcg
      901 cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta gtgaatttat
      961 atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt aatgttccag
     1021 tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg tcaggcttat
     1081 ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc tcaggtggag
     1141 gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta gcaacacaag
     1201 caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac cgtctaagca
     1261 atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga gtagctaaac
     1321 gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga attgaaatga
     1381 tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg atcaatgatt
     1441 ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa aacattgaaa
     1501 caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta aaaatgtatt
     1561 accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta tgcttacctt
     1621 ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt aaaaaaatat
     1681 tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc atggggaaaa
     1741 gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt gttgatacta
     1801 atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat aatagtctaa
     1861 cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc cctataaccc
     1921 atgaatttat tataaaagag gctttaaatt tagattgtaa tatctttacg gaaatcaatt
     1981 tattaaatga atattatgat gaaataatcg aagtagctaa aaagaaagat agattacttt
     2041 atctatcttc tacatttttg aagagaaaag aaattcaata tattaagtct aaagtgacgc
     2101 ataataaaaa aatttcttat caataccatg taggacagta tttacccgac tggcatcctt
     2161 gggaagatta tcgcaatttt tttgtttcaa acaaaaaaac taatgggtgt agagaaatat
     2221 tcgctattga attaccttgg attatagata catttgggaa agttaaatcg tttactgtaa
     2281 tgacccagaa attatccaat cttgatattg attattcgga cagtttcact cttattattg
     2341 agcatgaaac tggtatagtt ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa
     2401 gaaatttaac tataattggt gaaaagattt taatagaatg ggaaggtaac ccggattctc
     2461 tattttcatg gaatgaagaa acttctagta tgacagatat tcaattgtac gatgaggtta
     2521 tgcatgataa taattattca catactatta ttgaagatgc atatttagag gagattaaag
     2581 aatttttcac gcttttggaa aagaaaatgg aaccccaata tacttttgaa aaagataagt
     2641 atattattaa cttaattaac gacattgagg taaattaatg atgaaagtaa ttttttttgg
     2701 gttagggtca attggaaaga gacatctaaa aaatctaata agctatggaa aaaagaattc
     2761 tattaatttt gatataacag cttatagtag cagtggaaaa aaagagtttg aagaaataac
     2821 gtatattcaa aatagagacg atttggatac acattatgat atagctttta ttacaaatcc
     2881 gacttttaag catctggaa
//
LOCUS       DAZKCE010000184         2825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000184 DAZKCE010000000
VERSION     DAZKCE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.189"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8KQ_002823"
     CDS             complement(<1..461)
                     /locus_tag="ACG8KQ_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9105705.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(747..1033)
                     /locus_tag="ACG8KQ_002824"
                     /pseudo
     CDS             complement(747..1033)
                     /locus_tag="ACG8KQ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1023..1352)
                     /locus_tag="ACG8KQ_002825"
     CDS             complement(1023..1352)
                     /locus_tag="ACG8KQ_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9105706.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1429..1701)
                     /locus_tag="ACG8KQ_002826"
     CDS             complement(1429..1701)
                     /locus_tag="ACG8KQ_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9105707.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(1701..1964)
                     /locus_tag="ACG8KQ_002827"
     CDS             complement(1701..1964)
                     /locus_tag="ACG8KQ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9105708.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            2198..2825
                     /locus_tag="ACG8KQ_002828"
                     /pseudo
     CDS             2198..2825
                     /locus_tag="ACG8KQ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT          909 a          535 c          392 g          989 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      721 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
      781 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
      841 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
      901 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
      961 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     1021 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     1081 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     1141 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     1201 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     1261 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     1321 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     1381 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     1441 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     1501 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     1561 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     1621 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     1681 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     1741 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     1801 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     1861 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     1921 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     1981 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     2041 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     2101 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     2161 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
     2221 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
     2281 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagtaacg
     2341 cgatacagtg attattgctt gtgttatatg gggcataatt aatggctata ctagccaaag
     2401 agccacgtat agagcggttt gttccgtctt atttcctaat ggtgactttc ctagtaggag
     2461 tcgattcact cgcttaagtt caaacttagc ttacactatc aagattattc gatacttttt
     2521 catcaagaag ctcaccaaag gtgaactagt cggaattata gatagttttc ctagtccatt
     2581 atgtaaacca gttagaaata gacaagcaaa actattgaat caaatagcca aagttggcta
     2641 taactcaaca aaaaaatctt atttttatgg tcttaaaatt catatgatcg ttactaagac
     2701 tggctttccg attacttact caatcacgaa tccaggtgtc catgacgtga aagtgttaga
     2761 gactttatcg gaagaagcga atcttcccaa tatcctaggg gacaaaggat atattagcca
     2821 taaaa
//
LOCUS       DAZKCE010000185         2798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000185 DAZKCE010000000
VERSION     DAZKCE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.190"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8KQ_002829"
     CDS             234..1559
                     /locus_tag="ACG8KQ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9105709.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8KQ_002830"
     CDS             1552..1902
                     /locus_tag="ACG8KQ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105710.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8KQ_002831"
     CDS             1899..2069
                     /locus_tag="ACG8KQ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105711.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          986 a          442 c          539 g          831 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatattt
//
LOCUS       DAZKCE010000186         2578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000186 DAZKCE010000000
VERSION     DAZKCE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.192"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8KQ_002832"
     CDS             <1..1412
                     /locus_tag="ACG8KQ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9105712.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2578
                     /locus_tag="ACG8KQ_002833"
     CDS             1546..>2578
                     /locus_tag="ACG8KQ_002833"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9105713.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNL"
BASE COUNT         1011 a          360 c          518 g          689 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaa
//
LOCUS       DAZKCE010000187         2500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000187 DAZKCE010000000
VERSION     DAZKCE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.194"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..214)
                     /locus_tag="ACG8KQ_002834"
     CDS             complement(<1..214)
                     /locus_tag="ACG8KQ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105714.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPE"
     gene            complement(733..1218)
                     /gene="vanZ-A"
                     /locus_tag="ACG8KQ_002835"
     CDS             complement(733..1218)
                     /gene="vanZ-A"
                     /locus_tag="ACG8KQ_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9105715.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1371..2282)
                     /locus_tag="ACG8KQ_002836"
     CDS             complement(1371..2282)
                     /locus_tag="ACG8KQ_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9105716.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          758 a          468 c          364 g          910 t
ORIGIN      
        1 gttctggttc tgttggctca tttgggatta aatccactac tagcgttgag ttagttaact
       61 ttgcaatttg ttctagtgtt tttatggttg gatctgattt tcctgattct attcgtgaat
      121 aatttgatct actcatttct aattcttggg agaatttttc ttttgatttg gtacctctca
      181 tcttttttaa gctttctcta aatgctagtt ccaagtttct cccccctctt tttgtgtaat
      241 aaaattcacg ctttatttaa attatagctt ttaatatctt aataaacaag tgattaatca
      301 cttgtgattt gtgattattc caaaagtagg taatttcacc aacctttttc aaaagttttt
      361 atcgacgatt attggactgg atcgtgtttc ctaaagcgat tctaagcgca tgtttaattt
      421 taaagtgggt aattctcttt aaaacgtcct acaaagattc aaaaaaagca tggctgctag
      481 tgggggtacc gtcaggaaaa tgcggattta caacgttaag gaaatttatt aaagagacca
      541 attcctcagc actaaaattg ataattgggc tttctttgtt actatagtgt taggtaagct
      601 ttttacttta tcaccggttt tcccctcact tcacacctac gggcgagttt ccccacatcc
      661 cgttttccat ctaatccaat tcattcaata tctttatgtg tatgaaagat cagattgctt
      721 gatttagaca tcttacacgt aatttattct taaatgggta cggtaaacga gcaataatac
      781 gagcaaaagt atacctacaa aaataataac tctgtctaat tttttttgat tcatatgctt
      841 attgcttaaa ccatataatt tcagtccaag aaagcctcca acagtatttg taattacatc
      901 tgttatgtct gtcgctccaa tagcgaagat aaattgaatt atttcaaaag taagacttaa
      961 aaccagtaca aaagcaaact taggtaaaaa tccgatttct ttaaaattga cattcaaaag
     1021 caagccaaat ggaataaaga ttataacatt atctatcatc tctctgaaat tcccagtagc
     1081 agtaaatgga gtcaagttaa gacttctttg atgataatta aatactgata aaatattgta
     1141 ttgtaatttg aataacacta accagattag tgtcactaaa tataaagcta gcaatcctct
     1201 agataatatt tttcccaata taatttcctc taattctttt gtttaagttg tatcattagt
     1261 atagcaatgt cctagttaac gtgggtttta aattaaaaag ctaacgttac cccaaaagtg
     1321 ctagtacaat aatgctgttc attaatttcg ttggtttcat tccgccatcc ttacctcctt
     1381 gaattagtat gtgttgatcc gggaaacact gtcacaatta caccgtctat attgtttcct
     1441 gatatctcat aacgaagatt agtcggcaca tgaatggtgg tatttttagt aacaggataa
     1501 taaaagatct catatttttc cccatttaca ctaacagaaa tggttttttc ttcttttagg
     1561 taatccatat attcctcgag aacgaaattc ttttctttca taatcgcact atgtggtaaa
     1621 ccaacatagc gaatatgcca tggttcatat tgaattcctg ttaactctgt tttgtcctct
     1681 ggataacgta aaatgaaccc gtatttccaa gcattttctt ctatccactt tccttcaggg
     1741 gctcgttcca ttttcgtcaa gcttgatcct acatctagtg ataaacctga attatgctca
     1801 ctataacctg ctggtaaggc atactcagcc cccatttctt ggtaaagcac actttgctca
     1861 tcaaagtctc gatagccact attaataata aaatgactaa cgccaccctt tacagcatca
     1921 ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca tataaatatt actatcaagc
     1981 aacccgtatc catttattaa ttcgtcatgt ttagataaat tcacgatatc tgacttcaca
     2041 ctttcttggc gaacaggata tttactattg attaatagca gatttccttg ataaacctgt
     2101 tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc cactattttc taaatgttct
     2161 ttgggatttt gatcataatt ttgaaattcg actttttcct gagaaaacag tgcttcatta
     2221 acgtagtcat aacctaagta tattaagaat aacaataaca ataaaaaaaa caacttcttc
     2281 attttcagtc tcctccttaa ctatatagtt ggaagtatga aatatttcta gaatcttccc
     2341 cttaaacggc acaatctcgt ttgagattgt gccgttttgc cccgtaaatt acagtcaatt
     2401 atttcgccct actggattta ctattatcac caatgtagat gaattttcat aagtgatacc
     2461 catgctgaat ttaacgaggc tgaactgcct gttgacaatg
//
LOCUS       DAZKCE010000188         2478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000188 DAZKCE010000000
VERSION     DAZKCE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.195"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..903
                     /locus_tag="ACG8KQ_002837"
     CDS             <1..903
                     /locus_tag="ACG8KQ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP9105717.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..1824
                     /locus_tag="ACG8KQ_002838"
     CDS             916..1824
                     /locus_tag="ACG8KQ_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFP9105718.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1824..2444
                     /locus_tag="ACG8KQ_002839"
     CDS             1824..2444
                     /locus_tag="ACG8KQ_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105719.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          843 a          365 c          582 g          688 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagatgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataagaata cagaggttat ggaagcgact
     1021 atagttgaaa atggtcttcc ctacgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gtaggtggga aatatccagt ccaattagaa acaaaaattg tggatgctaa aaaagggaaa
     1141 attaactata tttttgataa atattccatg cagtgtttac atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctctta ttttgtcatt
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat cgaagattta
     1321 attgcggata tgacggactt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga acgttggaga caagaacagg aacacagttt ccaagattgg
     1441 agagaaggac aagaatcaga ctatttggca tggtttgaat caatcaagga tattttaaag
     1501 acgattgatc caggtggagt catgttagcc gaactaacgg atgcgcgagt agatattcaa
     1561 ggagttcgtc atgcgtctat ttcagagcgt ttgctggcag atatggacta tttatatcag
     1621 aaattgcgag caacgctttt cacgattgaa tacggggaga ttgaagtgac tgatattttg
     1681 caggatgatc tcttttcaga taatcatgaa gtcgaaaaag ttggaactgt agcattcccg
     1741 attgaagaag gagccttgat catcgcaacc gttgatgatc caaagcaaaa tgtgttcact
     1801 cttgagaaag ttggggtgat ctaatggcta aaactaaacg aatgatggaa acggatgaaa
     1861 aaacgggtgt acaacgccaa ttctttccaa tcacacatgc ttccgcagtt cttgggttag
     1921 aagaaataat agcaggagaa gcaacagttt tatctgttaa tggaaaaatt ggagccgtcg
     1981 tcattactaa agaagatttg ggattagaga atgttctcac agaattaccc tatgcaagtg
     2041 aagaagatga cggcattatc acggcagaaa tgtatcaaaa aattttaaac agtggagaag
     2101 gtgactacgt gttgccagtt gccactatcg accgtttggg tggcataaaa gttggtgaat
     2161 tattgacgat tgatgagaca gggaaagtct ctgcagtcag acaatctgat gtcaattttt
     2221 cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta tactgctgga gaaaacatca
     2281 ctattgatga agatggaaca attagttcta caggtggtag tgggactggt ggtgtcaatc
     2341 agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca agcagaaaat tacacaaacg
     2401 aaagaattcc aaattttact tttgaaaaaa ttggggaggt atagagaatg acagatatcg
     2461 taaaaataaa acaaagca
//
LOCUS       DAZKCE010000189         2459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000189 DAZKCE010000000
VERSION     DAZKCE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.196"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG8KQ_002840"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG8KQ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG8KQ_002841"
     CDS             1321..1779
                     /locus_tag="ACG8KQ_002841"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9105720.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG8KQ_002842"
     CDS             1938..2408
                     /locus_tag="ACG8KQ_002842"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9105721.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAZKCE010000190         2437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000190 DAZKCE010000000
VERSION     DAZKCE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.197"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..2290
                     /locus_tag="ACG8KQ_002843"
     CDS             137..2290
                     /locus_tag="ACG8KQ_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9105722.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSGFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          427 c          541 g          637 t
ORIGIN      
        1 tgattgctgt gaaacaaaaa gttgatttta tcttgaaatt aggtaaagaa tttcttgctc
       61 cgcccatttt ttctttttta ttaagaagtt tttgggtaca atgaagcatg aatgtaaata
      121 ggcggaagga taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt
      181 agaaaaaata agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt
      241 tccttttcgc ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc
      301 tagattgggt tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac
      361 ttctacgatt acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa
      421 agctttagtt acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac
      481 ggctaaagat ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga
      541 tgaaaatctg acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa
      601 agaagccaca aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag
      661 tgcgatggtc aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat
      721 tgctaccatc tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa
      781 ctctggtgtt actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg
      841 agtttcgact ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat
      901 tcttggcagt gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct
      961 agcaaaaggt tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga
     1021 agatgtcctg caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt
     1081 ttcgaatcaa aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa
     1141 tgcagaattc caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa
     1201 caacggcaaa gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg
     1261 agaagtgcta gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa
     1321 tgctttaggc acgattacta gcgcctttga tccagggtct gtagtcaagg ctggtacttt
     1381 gacagcaggc tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat
     1441 tcgtctgcaa ggtgcttcag aaaaaagctc tggctttaat cgaagcggac aagttgctct
     1501 tgatgcagtc aaagcatcgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa
     1561 gatgttagga ctagactata cacctggaat gggactacct agtttagatg aagaagctaa
     1621 agcttatcag caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat
     1681 cgatttgccg aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa
     1741 tgcggataat ggcaaagaat ggtatgctcc aggtaacttt accgatttgg catttggaca
     1801 gtttgacaca tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg
     1861 gaaacgagta cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt
     1921 gggtgaagtg aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga
     1981 aaatatggcg atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac
     2041 aacagccaaa ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga
     2101 aacggttgcg gaaggtcatc cagatattgc gacagtcaat agtaatatcg tggcatacgg
     2161 accaacagac aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca
     2221 agaccacata aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat
     2281 ggcaaaataa aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt
     2341 gtgaaaatag cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt
     2401 gaacaccgtt tactcggcaa aaaaggttgt gacaaaa
//
LOCUS       DAZKCE010000191         2427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000191 DAZKCE010000000
VERSION     DAZKCE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.198"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..980)
                     /locus_tag="ACG8KQ_002844"
     CDS             complement(42..980)
                     /locus_tag="ACG8KQ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9105723.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1471..2427
                     /locus_tag="ACG8KQ_002845"
                     /pseudo
     CDS             1471..2427
                     /locus_tag="ACG8KQ_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          839 a          422 c          390 g          776 t
ORIGIN      
        1 aattatactg aattagcaat ttttatcaaa taaaacttat tttacttcca aaacctaaat
       61 tcacgttgcc aaaaatcaat ctgcttttgc aattgttttt cgttcgcttt taaagtcgat
      121 ttcattaatt ccgttaaatc aattggagat atttctctaa tcaatttttt aaatttagtc
      181 ttagtattct tacttagctt tccccacata ctttcttcat gcaacaaagt ataaaccata
      241 gcttgctcat taattttttc taaagtagcc cacgcaggtt tcaagatgtg taaatcatta
      301 aaacaatcat tccagtaatc aaccatatct ctttttaatt caacttctac acgccataaa
      361 tgttcagaca caacttcaac atctgcgtta tctttacgtt cttgtttttt attataaatt
      421 ctaataaatc tatcactatc acggacacca aaatattttg tttctggctt gccattacga
      481 ccataaaaaa cagttttctt aactgcttta tcagtcattg catagtaatc gctcaaatca
      541 tcttcaaaat caaaagctaa gtctaatctt gtaaaaccgt catcttccat gtagtcgata
      601 atattttgtt ttaaccaaat catttcttca tgtgtgagtt tattgggatt aaattcaaca
      661 cgcatattac gtctatccca agtatctgct tttactttgt catattcgat ataaactttt
      721 tcttgaagtg ccttagcttt aaactttgtt tgaagtatat cccaaagtcg tatttgtggc
      781 tctacactca taaagtcaga tagcttttta gcattagttt tgttcaaatt tccaacgatt
      841 gtcatggcat caaaacttaa tgcgggttga gattttccca aagtttgacc acttaaccgg
      901 ctattactta accggctatt agagacggcc ggttttttta gtgagatttc tgcagctaat
      961 ttttcatcaa ttttcttcat tttttatcgc tcctaaaata atttttggaa cgaaaaaagg
     1021 acgagtggaa tcgcaaaata aatttcgcct gtatccattc gtccattttg ttttgtattc
     1081 aaaatcaaaa attgatatac taatcatcga atgaataaca agtgtggttt gttattccgg
     1141 gcgagaacgt tggtagcgtt gctcgtcttt ttttatgtct ttttttcgtt aaaactattt
     1201 taaaactaaa tatatcttaa agcaaataaa ttcaaggggc taaaagcgcc ccttaaaaac
     1261 cccttgcctc tgaaaagttt tgtgtttcct ttccgtcggt ttcaaaagct aatttactca
     1321 aaaaaataga tcaagggtaa atgcactcac tgcgttcgcc cttgagctat tttatttcgc
     1381 aaattttaac gcatttgccg acgaaaagat aaaacacata aaaatatacg cagatatatt
     1441 taaaacgtct tagaacgcaa aattttacgt ttaaaagcta tgcaaggact gtatgaagca
     1501 aaattattga gttatccaag aacagataca ccatttatta cagagaacga atttgcttat
     1561 ttaaaagcga attttggcaa atatagcggt tttttaggac ttgatcttga aatggttcaa
     1621 acagagccta gaaagcgtta tgtggacggt agtaaggtac aggaacacca cgccattatc
     1681 ccaacaaaac aagtacctac cgaatctgca ttagcgaaaa tggacgattt acaacgaaaa
     1741 atttatgctt tagtcgttaa aacgaccgtt gccatgtttt tacctgatta cttgtatgaa
     1801 gaaaccaaga tacaaacgaa agtagctgat ttgctgtttc aatcgatcgg aaagacacca
     1861 aagcaagaag ggtggaaaat tcttttcaaa caacaaacca aagaagaaaa agaggacgtt
     1921 caaacgttac cactcgttat cattggcgaa cgtgccgaag ttggtgttaa gagtgttgaa
     1981 aaagaaacgc aaccgccaaa agcttttaca gagggtacat tattaactgc tatgaaaacg
     2041 gcgaataaaa cggttgatga tgaagaagca atcaagattt tacaagaagt tgaggggatt
     2101 ggaacagaag cgacaagagc aagtattatt gaagcgttaa aacaaaaaga atatatccaa
     2161 gtgattaaga ataagcttgt tgtaactgaa aaaggaaaat tattgtgcca ggcagttgaa
     2221 agtcagcacc ttttaacgag tgctgaaatg acggctaaat gggaaacgta tttaaaaaaa
     2281 atcggtaaaa gagaaggcaa tcaagagaac tttattacga atatcaaaaa attcattgtt
     2341 catttactgg aagctgtacc taacgatata gaaaaactaa atttttctga ttaccaggaa
     2401 cagaaagaaa aagaagcaga aaaaagt
//
LOCUS       DAZKCE010000192         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000192 DAZKCE010000000
VERSION     DAZKCE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.199"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACG8KQ_002846"
     CDS             complement(<1..103)
                     /locus_tag="ACG8KQ_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9105724.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACG8KQ_002847"
     CDS             complement(135..458)
                     /locus_tag="ACG8KQ_002847"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9105725.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACG8KQ_002848"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACG8KQ_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAZKCE010000193         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000193 DAZKCE010000000
VERSION     DAZKCE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.200"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8KQ_002849"
     CDS             207..2069
                     /locus_tag="ACG8KQ_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9105726.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG8KQ_002850"
     CDS             2069..>2400
                     /locus_tag="ACG8KQ_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9105727.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKCE010000194         2368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000194 DAZKCE010000000
VERSION     DAZKCE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.201"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..543
                     /locus_tag="ACG8KQ_002851"
     CDS             289..543
                     /locus_tag="ACG8KQ_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9105728.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            544..861
                     /locus_tag="ACG8KQ_002852"
     CDS             544..861
                     /locus_tag="ACG8KQ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9105729.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1420..2355
                     /locus_tag="ACG8KQ_002853"
     CDS             1420..2355
                     /locus_tag="ACG8KQ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105730.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          847 a          334 c          449 g          738 t
ORIGIN      
        1 ttgtcggtct tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct
       61 atagaggatg ggcgacttct gcctaattct gttaaatgga tgttaacgga agtgtatggt
      121 gagtagttca ctcgtatttt gatatcgttt gtatatgttt tcagcgttct aagtggctta
      181 ttttgtccaa taataatatg gagtggaatc ccttaagaaa caccgttggc aattgttgtt
      241 ataacatgtt ataatttatt catcaaaaac taaaaggagt gtgataacat gggaaaagaa
      301 ctaaaggtta gaaagatcgg caatagcgtg ggagtgatcc ttccaagctc tcttggatta
      361 aaaagcggag acattatcca agccaaacaa gaaggcaact tattcatatt agatactact
      421 caaatagcaa aagaacacga tcgcaaattg attgaagaaa gtttccaaga ctttgagaaa
      481 ggtttaacag tttcagaaat tgaaatggta aaagctttcg gaaaatacgg ctggagtgaa
      541 tagatgaaag agtacacgct agtttattct aaaagttttc agaccagcct tgaaacgata
      601 atagaagaat gggaaaatga gcttgctctt tctccagcca gcataaaaag atttgtaggt
      661 gcgatccaga aggctttaga actaaccaaa aattttccaa ctatgcatga ggaagtttcc
      721 gaaatctatg gaatggatat cccaacatat cggatcgtga tcggcaaaag ctatgcctta
      781 ttttatcgga tcgaccatga aaaaaaagag atacttgtag gacgtatttt ccaatctaaa
      841 caaatgaaac taggctttta acaataatta ggaaaaagtc gctcatcctc tacaggtggg
      901 tgattaatgc cgctcgatat ggttgttaac cgtcggttac tcgaagattg atatataaga
      961 cttagtacct ttttattttg ctccatgtta tggccgaagg tctaaagata caatcattag
     1021 tctagagaaa aattaagaac cgtaggaact acggggatcg cttggtgaat aagagacact
     1081 gctattagtg aaaaaataag atatcaagta cgctctattc ccaaaaattt cgtgacttta
     1141 ctcatgagag gttcaagtag tatattttta atcaaaattg acgtaacttt taataaatac
     1201 tacctaaata aggtagtatt tatttttgtg ttttaaaaga ggaagtccaa tatatctatg
     1261 ggaaacttct taaatattat gttggtacat gaaaatatac atccctgcta gactatttac
     1321 aacattttac tttgtacgta ttcttgtttt aaaaggaatg ggataaaata taggtacaat
     1381 aactatttga tgctaaatat aattaatgga ggagagatag tgaacatact taatgaaaaa
     1441 agatatgtat ggtttgctgg taaaaaatat attactactt tcaaaagatt atccgagtta
     1501 atgcagcaga ctgttaaaag tgctatgcct gaaggataca agtttaattt tgttcaatct
     1561 tcagtatcta ctgaaagtat ctatttttca attctttctg agagtaggct ttttcatttt
     1621 agaataagtt ctcatgtaaa gaaaactgat gttgatatat atacgttccg tacaaataat
     1681 tataaaaatt taaatgattt gagtagaagt atcagtgaat atctcaaggg gaatagctat
     1741 ctcaagattc gattttcaca tatattaatt ttactaccat atttgaatga gaatttcacc
     1801 ctaattcaag tagataagtc tttcattgcc tcaggtaatc aaggaaatat gaatattaca
     1861 agtaacaaaa cgtcacgtgt gctattggcg ttgatgggtt acggaattct ttatagagat
     1921 gaccaaaatg gccagattct aataaatcgg gctgggcgtt cattattaat gaaatataga
     1981 aaatttatac cggaagattt tcaaaactta cagttagaaa acatgcgttt agaagattta
     2041 tttagattag cagaagatga taagtataga tttgaagttt tgaaaattca aaagaatgaa
     2101 ctattgaata agttatatcc tgagaatgcg ataaaagcac attttggtga tgctaatcat
     2161 atgtatagtt ggttcattcc taaaagattt agagggaaaa tcaatgctgg agatcaagtg
     2221 aaagtactca atcgaaatag tacagatgtc gtaatgataa cagatatcta tgtagaagat
     2281 gaagatataa tcaaatatat gaagcctgtg ataaaacttg ttaataaaaa taaaggaacc
     2341 cgtggtgcta gttaattttt tcctagag
//
LOCUS       DAZKCE010000195         2307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000195 DAZKCE010000000
VERSION     DAZKCE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.202"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..721
                     /locus_tag="ACG8KQ_002854"
     CDS             527..721
                     /locus_tag="ACG8KQ_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105731.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="ACG8KQ_002855"
                     /pseudo
     CDS             912..1161
                     /locus_tag="ACG8KQ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="ACG8KQ_002856"
     CDS             complement(1405..2235)
                     /locus_tag="ACG8KQ_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9105732.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          692 a          418 c          460 g          737 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcg
//
LOCUS       DAZKCE010000196         2262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000196 DAZKCE010000000
VERSION     DAZKCE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.203"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(856..1281)
                     /locus_tag="ACG8KQ_002857"
     CDS             complement(856..1281)
                     /locus_tag="ACG8KQ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFP9105733.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(1383..2000)
                     /locus_tag="ACG8KQ_002858"
     CDS             complement(1383..2000)
                     /locus_tag="ACG8KQ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9105734.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          737 a          435 c          243 g          847 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttgggttt ctttatattt tcatatttaa tagacattac catataaaca
     1621 tatggaataa aaagaataat attttctaaa cacgctgtag ataactcttt tgttccagtt
     1681 gttgtattat acttataaat ccaccagtct cccataacat cagataaagg atttaaccaa
     1741 attgttcgat tgattaatgt tgtaaataaa atcatcgcag tatagaatat caaagcacaa
     1801 acttttctat attttctatc cttctttaaa tgaagataaa gttccattag tatacgttta
     1861 tagccctcac ctgcagcaat gccatctgtt gcatacataa aatagaccag tcctaaaatt
     1921 gtcaataaaa cagcaaaccc aaagggttca tataatttat ataatatatc tgtaataatt
     1981 tctgcaatca ctattatcat aattatttct ccaatatcaa gtatacactt atcgcttcca
     2041 attacacatg atcatattag agtgtattat cacattctct aatattcaat ttatttttcc
     2101 taaaatattc gaaataatcg tcttgataac gagtaagtat ttttatacga ttgataacat
     2161 agtaacaata tttttaatta tagtaaaaaa tcatctgcct ttttcatcca cctaatctta
     2221 tttttcattt tattcaacat ccttaaataa acttccgttc ct
//
LOCUS       DAZKCE010000197         2233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000197 DAZKCE010000000
VERSION     DAZKCE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.204"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            790..2178
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_002859"
     CDS             790..2178
                     /gene="ltrA"
                     /locus_tag="ACG8KQ_002859"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9105735.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          775 a          398 c          497 g          563 t
ORIGIN      
        1 tttttccatc aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt
       61 tttttgtcgt ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa
      121 cgaattagat atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga
      181 taaacattga taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg
      241 tacggataca ttttcggacc gattaatttt aaccattcta tttatttgct tacctattag
      301 atatcaataa aaaactgctt aacagctatt tcagctgtta agcagtttta ggatgtgcgc
      361 cctgcatggg tgaaaacttg gtggtgaaag tccactacag gcttggcagt aggaactgtt
      421 agcgaaagac aaggcggcaa tcgtgaggtt gtggctgaag gaagtcggac gcaaactcct
      481 gaactgacga acagaaacta gatacaaggc tgaattaggt cggataagtc ttcatcacaa
      541 gacaaagtcc aatactgccc gaaacctata cagtaaatct agcagttaca tgggaggaaa
      601 gttttcactc ttaccggggg agatctcatg agggtgttgt gggcgttgaa tgaaaacaaa
      661 ccagcacaac gatcatggcg acatgatggt gaatcatgag aagtcagccg aggtcatagt
      721 agtaacgaca ctgaccgtta cgaaggactg aacaataaga atcttgaata atttaggagg
      781 tgtgagaaga tgcgacgacc gcagaaaaca agaaaacttg gctatcatca aagagctagt
      841 gtggaacacg aagggtatga tgaagtgcgc agcacttctt gcggtgaaca gaggaaacaa
      901 gatggtgtag aaaatctgtt tgggagaatt ctagatcgga ataatctcaa tcaagcgtat
      961 cttcaagtag tcagaaataa aggggcggct ggtgcagatg ggatgaccgt cgatcaactg
     1021 cttccttact tgaaagagca ccgcgaggaa ttactatcca atttacgcca agggaactat
     1081 aaacctcagc ctgttttgcg agtagaaata ccgaaaccaa acggcggtgt aagaaaacta
     1141 ggaattccca cggttattga tcgtatgctt caacaggcaa tcaatcaagt gttacaaccg
     1201 ctatttgaac cagaattctc agataatagt tatgggtttc gcccaaatcg cagcgcacat
     1261 gacgcagtga agaaagcaaa atcttattat gaacaaggtt accgatatgt ggtggacatt
     1321 gatatgaaag cctactttga caccgtaaat cacgataagc tgatgtattt cattgagcgg
     1381 aaagtaaagg acaaacaagt cctaaaactg atccgtcaat acttaatgag tggtgtaatg
     1441 gaaaatggac tcatcacaac aactgaagaa ggaacgcccc aaggaggcaa tctctctccc
     1501 ctgttaagta acatctacct gaacgaattt gatcaagtgc tagaaaatct aggacataaa
     1561 tttgttcgat acgccgatga ctgcaatatc tatgttagaa gcaaacgagc aggacagcgt
     1621 gtgatgaaga aatccattca atttctcgaa gaacaactaa agttgacggt aaatcgagaa
     1681 aagagtgcgg taggaagtcc attgaaacga aagtttcttg gattctgcat tctgccaaca
     1741 aagaaaggta ttaaaattcg ccctcacgca aaagcaaaac agcgtgtgaa aagcaagctc
     1801 aaacaactca ctaaaagaaa ccgtggaaga agtatccaac ttattttaaa agaaatcaag
     1861 caactaatga ctggttggat caactactat ggaattggtg agatgaaaga attcatgagg
     1921 gagttaaacg gttggttgaa gcgaagaatc agacaatata tttggaaaca atggaagaat
     1981 ccaagaacca aaagaaaaaa tctgattcgt ttagggatcg ataaagcgaa agcgtatgaa
     2041 tggtcaaata caagaaaagg tatttggtca gtctccaaaa gtcatattct tcatcgatca
     2101 ttaacagaca aagaactggc ccgccaagga tatgaggaca tctccttgaa gtaccagttc
     2161 gtacactcaa cttattgaac cgccgtatac gggtccgtac gtacggtggt gtgagagggg
     2221 cggaaggtca gtt
//
LOCUS       DAZKCE010000198         2227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000198 DAZKCE010000000
VERSION     DAZKCE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.205"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..394)
                     /locus_tag="ACG8KQ_002860"
     CDS             complement(95..394)
                     /locus_tag="ACG8KQ_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9105736.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            745..1377
                     /locus_tag="ACG8KQ_002861"
     CDS             745..1377
                     /locus_tag="ACG8KQ_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9105737.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1500..2138
                     /locus_tag="ACG8KQ_002862"
     CDS             1500..2138
                     /locus_tag="ACG8KQ_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9105738.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          741 a          358 c          326 g          802 t
ORIGIN      
        1 ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta
       61 tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg
      121 ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat
      181 aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt
      241 ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta
      301 tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta
      361 gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt
      421 cttcccacta cattctataa acaaagtata cgctacatct agattttttt aaatcattac
      481 tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc
      541 taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt
      601 gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa
      661 ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact
      721 ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat
      781 aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca
      841 caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc
      901 aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc
      961 gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt
     1021 gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc
     1081 gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt
     1141 gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa aataatgttt
     1201 tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa tatcgattta
     1261 agtagcaact cctttgaaat gattgaagta cgctcggaac atctaaaaaa ttgtacgctt
     1321 aatcagttgc aagcacttgg ttttgctaga aaatttttac aaataaaagt agtataatca
     1381 aaatgaatat ataagataaa tttttagcaa tacaaatatc cataaaaaaa cattgcctat
     1441 tttaactctt catgtatact gactctatct tcaaaaactg aatagtagga gttattatca
     1501 tgaataacat tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc actattgtcg
     1561 ttgtactcgg tttgttaggg ttgctatttg cgccatccat tattagcatt ttttctttaa
     1621 ctatcactgg atttatagca tttacacttg tttttggtaa aaaagatacg aaaaaaatgt
     1681 tctcgaaacc tgtcgctcct gtcaaaaata tagccaaata tttttttatc aatgtagtaa
     1741 tcagtgccgc agtctcggta tttttgcaac aaattttgaa atggggttta acaggcaatc
     1801 caataaatga ggctttcagc ccgttattat ttattatttt gcctatcatg atacttggag
     1861 aagaattgtt ctcagtgtat tttctcgcta ttttttctag taagttcagc attcctgtcg
     1921 ctagtttctt gtcagcaatc atctttggat ttatccatta cagtacttat gataatggaa
     1981 atattttaca cactgtagca cacatccttt tgatacaagg ggtggcaaga ttgttattca
     2041 accaagctgc cattaaaagc aactcgatca ttacaagttg ggcagttcat gtgatatttg
     2101 atttcacagc tatattgcta gtcgttttat tctcctaaat ttttatagaa ataatacatg
     2161 ctctattgct aagaaagcat actttatttc ctttaaacaa aaagacagat gtatcaatgg
     2221 attaaaa
//
LOCUS       DAZKCE010000199         2198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000199 DAZKCE010000000
VERSION     DAZKCE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.206"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..256
                     /locus_tag="ACG8KQ_002863"
     CDS             <1..256
                     /locus_tag="ACG8KQ_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9105739.1"
                     /translation="IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPE
                     SANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            257..1009
                     /locus_tag="ACG8KQ_002864"
     CDS             257..1009
                     /locus_tag="ACG8KQ_002864"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9105740.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1014..1961
                     /locus_tag="ACG8KQ_002865"
     CDS             1014..1961
                     /locus_tag="ACG8KQ_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9105741.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          769 a          287 c          433 g          709 t
ORIGIN      
        1 aattcattta gattattttg gaagagttcc tacaagaaaa tttcaattgt ttactttaga
       61 tgaatgtatc aatgttgact tattaaataa taaaataagt tttgaagaaa acattgttaa
      121 agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt tctttgaaaa
      181 cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag tattgaaaat
      241 agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg ggtctaaggg
      301 gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt tcgctttagc
      361 aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt gtttgaatac
      421 ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga aaattgtaga
      481 acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac gtgattcatt
      541 cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag atttgacttc
      601 acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa aaaatttaga
      661 taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta atatggtaaa
      721 aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta ggcaacagac
      781 acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa
      841 aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt
      901 agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga
      961 agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct
     1021 gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat catttaggag
     1081 aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata
     1141 tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga
     1201 tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag
     1261 attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt
     1321 tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg
     1381 ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga
     1441 cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat
     1501 atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga
     1561 attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag
     1621 gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata
     1681 tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac
     1741 aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg
     1801 aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg
     1861 actctctagc agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt
     1921 taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt
     1981 agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg
     2041 ggatagaagg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa taatttggac
     2101 agaacagaaa gaagaaaacc tctttctgtt ctgtttattt ggttaaaaat gttttgccat
     2161 gacgaatcat aattcgtagt ttgaaattgt aaaagttg
//
LOCUS       DAZKCE010000200         2176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000200 DAZKCE010000000
VERSION     DAZKCE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.207"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8KQ_002866"
     CDS             <1..878
                     /locus_tag="ACG8KQ_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9105742.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            1035..>2176
                     /locus_tag="ACG8KQ_002867"
     CDS             1035..>2176
                     /locus_tag="ACG8KQ_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9105743.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQI"
BASE COUNT          679 a          448 c          431 g          618 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      961 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
     1021 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
     1081 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
     1141 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
     1201 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
     1261 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
     1321 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
     1381 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
     1441 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
     1501 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
     1561 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
     1621 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
     1681 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
     1741 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
     1801 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
     1861 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
     1921 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
     1981 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     2041 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     2101 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     2161 ttctgccaca gatcca
//
LOCUS       DAZKCE010000201         2127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000201 DAZKCE010000000
VERSION     DAZKCE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.208"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8KQ_002868"
     CDS             complement(<1..914)
                     /locus_tag="ACG8KQ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9105744.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8KQ_002869"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8KQ_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9105745.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          721 a          374 c          411 g          621 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatat
//
LOCUS       DAZKCE010000202         2125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000202 DAZKCE010000000
VERSION     DAZKCE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.209"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..>2125
                     /gene="acm"
                     /locus_tag="ACG8KQ_002870"
     CDS             15..>2125
                     /gene="acm"
                     /locus_tag="ACG8KQ_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9105746.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIG"
BASE COUNT          837 a          343 c          414 g          531 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatcatt ggttt
//
LOCUS       DAZKCE010000203         2114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000203 DAZKCE010000000
VERSION     DAZKCE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.210"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8KQ_002871"
     CDS             278..820
                     /locus_tag="ACG8KQ_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105747.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACG8KQ_002872"
     CDS             976..1425
                     /locus_tag="ACG8KQ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9105748.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2114)
                     /locus_tag="ACG8KQ_002873"
     CDS             complement(1455..>2114)
                     /locus_tag="ACG8KQ_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFP9105749.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          752 a          336 c          399 g          627 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcg
//
LOCUS       DAZKCE010000204         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000204 DAZKCE010000000
VERSION     DAZKCE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.211"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG8KQ_002874"
     CDS             100..570
                     /locus_tag="ACG8KQ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9105750.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG8KQ_002875"
     CDS             792..1895
                     /locus_tag="ACG8KQ_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9105751.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKCE010000205         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000205 DAZKCE010000000
VERSION     DAZKCE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.212"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8KQ_002876"
     CDS             <1..378
                     /locus_tag="ACG8KQ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9105752.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8KQ_002877"
     CDS             426..1826
                     /locus_tag="ACG8KQ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9105753.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKCE010000206         1989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000206 DAZKCE010000000
VERSION     DAZKCE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.213"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..411
                     /locus_tag="ACG8KQ_002878"
     CDS             175..411
                     /locus_tag="ACG8KQ_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9105754.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            430..645
                     /locus_tag="ACG8KQ_002879"
     CDS             430..645
                     /locus_tag="ACG8KQ_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFP9105755.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1348..>1989)
                     /locus_tag="ACG8KQ_002880"
     CDS             complement(1348..>1989)
                     /locus_tag="ACG8KQ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9105756.1"
                     /translation="KTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKY
                     DVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATK
                     QVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYP
                     MLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          647 a          309 c          351 g          682 t
ORIGIN      
        1 ttccacaaaa actttgaaaa cagttcttga acactaactt tttcattcct gaatcaaatt
       61 tattttggaa attttactat tcaagaataa acttgataag tttcaggaag atttttactg
      121 ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt
      181 aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg cacaaactgt tggtggaggc
      241 aataatgctt ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt
      301 ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg gtgttgttgg agggttatat
      361 ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga
      421 ataagtagta tgcatattaa aaacacaaaa acaacattta tattatcttc agaagagttg
      481 aaaaatattc aaggtgggag tgccgtggga gttttaggca ctactttttc tggggcaaca
      541 gcaggagtaa aactttgttc agccggagga ccttatgcaa tagctgcttg tggtgtaggt
      601 ggagctttat taggtgctgg attcagcatg tggaccggtg cgtaaacaat taaaaaatat
      661 caaacctcaa gattggtttg tgtcgattac gaacataggt atgttcttta tcttttcaaa
      721 agtttggaat ggacagaact ttttactaaa gcttcttgtg ctagttttta taattatatt
      781 aaatttttat cttttattaa agtcagataa aaattctttg gaaatgtaat ggtagagata
      841 tttttacaaa aggacttgca gcgctgtagt tggtgctgca ggaggaccta tttcggccgt
      901 tacaggaggt attggtaatg tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta
      961 aatttataaa aacaggaaag taattatctc aaatcctatc aagtacaatc ataggattgc
     1021 acttgacagg acaaattttt taactaaaaa atgaaaaaca ctttctaaaa attagtagaa
     1081 tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct ttatggtact
     1141 taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga ccatagaaat
     1201 attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta taaaaattgg
     1261 tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt ttattagaaa
     1321 aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat
     1381 tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg
     1441 ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct
     1501 tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc
     1561 atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa aataaatgtc
     1621 atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt gattcgtaac
     1681 ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa tctcgtttcg
     1741 atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc ttctttttat
     1801 tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt gaacatcata
     1861 ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat caatatctaa
     1921 gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt ctaacggact
     1981 ttgtgtttt
//
LOCUS       DAZKCE010000207         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000207 DAZKCE010000000
VERSION     DAZKCE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.214"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8KQ_002881"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8KQ_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8KQ_002882"
     CDS             complement(728..1744)
                     /locus_tag="ACG8KQ_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9105757.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKCE010000208         1940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000208 DAZKCE010000000
VERSION     DAZKCE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.215"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACG8KQ_002883"
     CDS             <1..106
                     /locus_tag="ACG8KQ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9105758.1"
                     /translation="LFLDFPFLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            103..>1940
                     /locus_tag="ACG8KQ_002884"
     CDS             103..>1940
                     /locus_tag="ACG8KQ_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9105759.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          721 a          237 c          385 g          597 t
ORIGIN      
        1 cttattttta gattttccat ttttatatcc tggaaaaaat gaaattaaat ggacgaaaaa
       61 tattcattca tttgaaataa tgcctaggtg gtggagaaaa gtatgaaacc aagaatatat
      121 agtcctactg aaacagattt ctcaacgaat ggcttaggaa ttttaaagga ttgtacaaga
      181 tgtggaatat atgaagtagc taatggaaaa tatgaattag agttggatta tcctttagga
      241 actagatttg atgaatattt tgaaaatgac tatcaaataa aagcaaagcc aaacgatcaa
      301 gaagagtatc atatcttttt tattgatgat aaagatatag atactttttt aaatacagta
      361 actatttatg ctcagagtcg tacaaatcga cttggaagac gggtagtcac tcttgtggaa
      421 gtggactcta aaactggtca agaagcgata tcaattattg aaactaaaat ggataaaaaa
      481 tctgacatac gactttattc tgatattacg gctgtttcta gcacaatctt tgaagcgaga
      541 aatgttttaa attgtattgc tggtgaacaa ggatcattgc ttcaatattg gggcggagaa
      601 attaaacgtg aaccatttaa gctttctttg ttaaagcgaa gaggtcgcaa taatgttgga
      661 acgattcggt atggaaaaga tatgtctggt ttgaaggtca aattagattg gacaggtata
      721 aaaacaagaa ttattccata tgctgatcct caaagtgaag taggtacgac taatcgaatt
      781 tatggttcgc cagtagatag tgcctatatt aataactatc ctgatgtcta tacagaacat
      841 gttcagttta cagaagaaca aggagtaaaa gacattaaga gcttaaataa aatagctaaa
      901 aattacttta aaacgatcaa tcctggctgt gataagccta aagtttctat tacggttgaa
      961 tttgataagt tgactgatag tgaagaagcg aaagaatttg cgaagattag aaattatggt
     1021 ctgtttgata catttaaaat atatcataaa aaatataata tttatcttga atcaaaggtt
     1081 agtggactac aatacgattc tttatctgaa aaaactttga aattagaagc aggagacatt
     1141 caagttgctt tttatcagca acaagctgta actattcagg ataaattaaa agattatgca
     1201 acgaataatt atatgagtga ttttaatgat tatgtttctt caatgattac aggtcaaggc
     1261 agtgcaggag gatatgtagt tttatggcca aaagaaaaac cttccaacat ctttattatg
     1321 gataatccag atttagaaaa ggcaagagaa gtactaagaa tgaataaaaa tgggattgct
     1381 ttttctaaga aaggatggcg ggggcctttt aattcagctt ggacattaga tagtatattt
     1441 aatgccaatt ttatacaaac tggattaatc aaagcagata tctttcaaaa ttcttttaac
     1501 aagactggag atgtattgaa attagttaat ggactactcc aaatttggaa caacaaaaag
     1561 aaaataatgg agttaactaa aaaaggaatg gaattttgga attctaaggg ttcaattgga
     1621 acgattggaa caactgattc tgttggtaat ccttttcctg gggcttctac tccaacccct
     1681 attgaagata attctttagt tattcgtaca aatggagacg gcaaatatat tttgatttct
     1741 cctaaagtag gaaaaggatt agttttatta ggaaatggta aagcaattta ttttggagac
     1801 ttagatgtac aaggaaaact tacagttaaa ggaaaagaaa taattcctgg acaaggtggt
     1861 ggtccatcgg gaggtggaga atctactggt gggtatccta gtgaattaaa aacagatgca
     1921 gaaaagagag cttggagaat
//
LOCUS       DAZKCE010000209         1868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000209 DAZKCE010000000
VERSION     DAZKCE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.216"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1295
                     /gene="tgt"
                     /locus_tag="ACG8KQ_002885"
     CDS             150..1295
                     /gene="tgt"
                     /locus_tag="ACG8KQ_002885"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9105760.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1392..1772
                     /gene="yajC"
                     /locus_tag="ACG8KQ_002886"
     CDS             1392..1772
                     /gene="yajC"
                     /locus_tag="ACG8KQ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9105761.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT          642 a          311 c          410 g          505 t
ORIGIN      
        1 ttttcttttt tttgttattc ataaaaaaag gataaaaaaa agaatattaa ataaagtaga
       61 aggaagccga gggaacttgc aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa
      121 gatattttat atagaaagta gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa
      181 tcaagaaaga aaaacatact ggcgcgcgtt taggggaatt gatcacgcca cacggaactt
      241 tccccacacc aatgttcatg ccagtaggaa cattagctac agtaaaaaca atgtccccag
      301 aagagttgaa agaaatgggt gctggcgtta ttttaagtaa tacatatcat ttatggctac
      361 gccctggtga agacttagta gaagaagctg gcggactgca taaatttatg aactgggatc
      421 aaccgatttt aacagattca ggcggctttc aagttttttc attaagtgat atgcggaata
      481 tcgaagaaga aggcgtgcat ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac
      541 cagaaaaagc aatcaacatc caaaataaat tgggctctga tatcatgatg agttttgacg
      601 aatgtccacc attcgatgaa agttacgact acgtgaaaaa atcagtagaa cgtacgtctc
      661 gatgggcaga acgaggattg aaagctcatg ctaatccaga ccgtcaagga ttatttggaa
      721 tcatccaagg agcagggttt gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga
      781 tggatttccc tggttactca atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga
      841 atcgtgtatt agaattcact acaccgttga tccccgaaaa caaaccacgt tacttgatgg
      901 gtgtcggtgc tgctgattca ttgatcgatg gtgttatccg tggagtagac atgtttgact
      961 gtgtcttgcc tactcgaatc gctcgaaatg gcacatgtat gacttctcaa ggccgtctag
     1021 tagtgaaaaa tgcgaaatat gcccatgatt tccgtccaat tgatgagaaa tgtgattgtt
     1081 atacatgtaa aaactataca cgtgcctata tccgccattt gatcaaatgt gatgaaacat
     1141 ttgggattcg tttgacttct taccacaatt tatacttctt gttgaatcta atgaaacaag
     1201 tacgtcaagc aatcatggat gataatttgc ttgaatttag agaagccttt tttgaagaat
     1261 acgggttcaa taaagaaaac gcaaaaagct tctaaaggaa aatcttttaa aaattaagta
     1321 aaaaactagt gttatcagca tttttctgct aaagtgtttt ttgtagatac agaattagtg
     1381 aggtgaaaaa aatgggaaca ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt
     1441 ggttttttat gtcacgttcg caaaagaaac aacagcaaga acgtcaaaac ttgttagata
     1501 gcatgaaacc aggtgattca gtcgtaacga tcggcggatt acatggagtt gtttcagaaa
     1561 tcgacaatga caaacggaca gttgttttag actgcgaagg gatcttcctt gagtacgatc
     1621 gtgcagcaat caaaacggtc aaaccaggga ctgtcgtaac aaatgatgca acgactactg
     1681 ttgaaacaac agaagaacca gctgagacag ttgaagtaaa aactgaaaca acagaaacgc
     1741 tggaagagac agaagagaag gaagaaaaat aagtatcaaa gcttatttaa agacgaggta
     1801 gggcagaaag atgtcctacc tcatttttta ttcaaaaaag aaagagataa gagcatggga
     1861 tatattgc
//
LOCUS       DAZKCE010000210         1863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000210 DAZKCE010000000
VERSION     DAZKCE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.217"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..724
                     /locus_tag="ACG8KQ_002887"
     CDS             <1..724
                     /locus_tag="ACG8KQ_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFP9105762.1"
                     /translation="DFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGL
                     PVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPV
                     GYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTE
                     IFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQ
                     YIGKKVKRPISADSVLLEEDVE"
     gene            721..>1863
                     /gene="neuC"
                     /locus_tag="ACG8KQ_002888"
     CDS             721..>1863
                     /gene="neuC"
                     /locus_tag="ACG8KQ_002888"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP9105763.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIA"
BASE COUNT          681 a          243 c          364 g          575 t
ORIGIN      
        1 tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac aaattggtgt
       61 acaaggattt aaaattggct caggagattt aacaaattta ccttttctta gaaaaataga
      121 tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg aggtcagtga
      181 ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt caaattatcc
      241 ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa tgttcgggat
      301 acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg ctgttgcgat
      361 gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg gtccagatca
      421 taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta gaaacactga
      481 aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta caaaagaagt
      541 atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc ttacagagaa
      601 atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat atcaatatat
      661 tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg aagatgttga
      721 atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata taaacctgtt
      781 gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg aatgcattta
      841 tcccataaat atggatttac taaagaattg atcttagctg acggatttag aattagtgga
      901 gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc tgtggcatta
      961 gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt attattacta
     1021 ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca
     1081 gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag acatgccata
     1141 acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa
     1201 atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga tactatttta
     1261 aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa atttgaaaag
     1321 aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa tatagaaaaa
     1381 caaattttag ctttgatcaa ttcaatggaa caacaatctc aaccaattat ttgcatttta
     1441 cctaatagtg atgctggtag cgaaattatc agaaaaatgt accagaaata tttaaaaacg
     1501 gatattattt atattacgaa tttagagccg gaggaatacc tgactatatt aaaagagtgc
     1561 aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt caaaatacca
     1621 gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat tataaatgca
     1681 aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc tcagtttata
     1741 aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag aagaataaca
     1801 aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca aaaacaaata
     1861 gct
//
LOCUS       DAZKCE010000211         1855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000211 DAZKCE010000000
VERSION     DAZKCE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.218"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..561)
                     /locus_tag="ACG8KQ_002889"
     CDS             complement(52..561)
                     /locus_tag="ACG8KQ_002889"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9105764.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(673..1671)
                     /locus_tag="ACG8KQ_002890"
     CDS             complement(673..1671)
                     /locus_tag="ACG8KQ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9105765.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          562 a          352 c          332 g          609 t
ORIGIN      
        1 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
       61 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
      121 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
      181 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
      241 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
      301 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
      361 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
      421 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
      481 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
      541 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
      601 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
      661 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
      721 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
      781 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
      841 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
      901 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
      961 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
     1021 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
     1081 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
     1141 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
     1201 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
     1261 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
     1321 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
     1381 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
     1441 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
     1501 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
     1561 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
     1621 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
     1681 tcccttttca cactatagca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
     1741 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
     1801 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaat
//
LOCUS       DAZKCE010000212         1816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000212 DAZKCE010000000
VERSION     DAZKCE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.219"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8KQ_002891"
     CDS             <1..828
                     /locus_tag="ACG8KQ_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9105766.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG8KQ_002892"
     CDS             828..1445
                     /locus_tag="ACG8KQ_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105767.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1449..>1816
                     /locus_tag="ACG8KQ_002893"
     CDS             1449..>1816
                     /locus_tag="ACG8KQ_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105768.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          660 a          259 c          387 g          510 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcgg tgtacaggtt tatccacaaa
     1501 ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg
     1561 gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct tctgttacga
     1621 atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga gataaaggtg
     1681 atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat gggttgatgt
     1741 cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa aatccaggta
     1801 atgctacaac tacgac
//
LOCUS       DAZKCE010000213         1755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000213 DAZKCE010000000
VERSION     DAZKCE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.220"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACG8KQ_002894"
     CDS             <1..617
                     /locus_tag="ACG8KQ_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105769.1"
                     /translation="KKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGI
                     EEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEK
                     NILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTAL
                     SEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            604..>1755
                     /locus_tag="ACG8KQ_002895"
     CDS             604..>1755
                     /locus_tag="ACG8KQ_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9105770.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          584 a          224 c          280 g          667 t
ORIGIN      
        1 tcaaaaaaaa aattgacgat tattttgtct attgtccgga attgttactt tatgatgtaa
       61 aaaatataaa aaaaattccc aaaatagatg aaaacagtaa attgatgaaa aacatacaat
      121 caatctttgg gatagaagaa gtttttgtcg gagataaatg tatctttttt gatcaacctt
      181 tattatctga tggttatggc atagatgaaa cagatatttt cgctaaagta aatagtatat
      241 tttctggaaa aaaagaaact ttaattaagc ttcatcctag agcaaggaca aatcgttatg
      301 gtgaaaaaaa catattgaat tccagtttac cgtttgaagt cttgctactt gaaaatgact
      361 tctcttcaac acttttgatt agtcctatat ctacaatttc tttttcacca tctatgatgt
      421 ttgaaaagac gatcaactct attatgttgg ctaaattaat tttaaatgaa tatacagcat
      481 tatctgaaga taaaaaagaa acattgacaa atattgttct tttctgtgaa cattataacc
      541 gtctatcaga agaaaaaatt ttactcccag aaaactgggc agaattaaga aagttggtag
      601 agtatgggat tatttaacac actttccaag tcaaaattag ttaagtccag tttctggtat
      661 actataggga atttttttat taagggaatt tcattcataa ctattcctat ttttgtaaat
      721 attactactc aagcagaata tggtttgatt aataatttca cttcatttgt atcaattttt
      781 tcgattcttg ttgggctaag cctaaattcg gcaattaata atgcaagttt tgactataaa
      841 gatgacataa atgaatttat gtcatcggta ctttctctag ctacactttc gttaattgtg
      901 ttcttattaa tagggaacat atattttttg ttttcaaatg ctttttttga tatgaaccaa
      961 cttgtattta atttgatgtt attacaaagt tttgctagtt ttgtaattag ttatatccag
     1021 tcttatttta cgattaactt taaccatttt aaatatttga ttttatcttt tatatctact
     1081 tttttgaata ttgcaatctc tgtagctttg atgcagacat tatattttga tgataaatat
     1141 gttggcagag tagtcggcgg aacactgtca tttgtgttga taggtttagt aatttattta
     1201 agaataatga taaagggtgc tcattttttt gataaaaaac agtggagata cgctttaaaa
     1261 atctctttgc cgctaatccc acattcctta gcaaatatag ttttagcaca attcgataga
     1321 attatgatta attcttattt aggtcctgct gcggggggaa tatattccta cattagtaac
     1381 ataggtatta ttctaagcgt aatatggatg tctactaata acgcctgggt tccatggttc
     1441 tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa ctattattta
     1501 gtagtcttta ctttgattac gatgattgta atggtagttt cgattgattt agcaaaattt
     1561 atggctccga aagactatta ttctgggtta tctttagtgg ttccgataac cttaggctat
     1621 ttctttcagt ttttatatag tttaccagtt aatgctgaat tttatgagaa aaaaacatcg
     1681 tttattgcga ttggtacagt agcaagtgct atagttaata tactattaaa catgattttc
     1741 atacctaggt tagga
//
LOCUS       DAZKCE010000214         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000214 DAZKCE010000000
VERSION     DAZKCE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.221"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8KQ_002896"
     CDS             131..505
                     /locus_tag="ACG8KQ_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9105771.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8KQ_002897"
     CDS             complement(619..1248)
                     /locus_tag="ACG8KQ_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9105772.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8KQ_002898"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8KQ_002898"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKCE010000215         1650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000215 DAZKCE010000000
VERSION     DAZKCE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.222"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..416
                     /locus_tag="ACG8KQ_002899"
     CDS             <1..416
                     /locus_tag="ACG8KQ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105773.1"
                     /translation="KDCIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILEN
                     EIFVNKTRRLLLGRCRTCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQ
                     NPIKQRIFNYFLLNQSIIGVKTLAYLSGMGRNRRF"
     gene            734..1324
                     /locus_tag="ACG8KQ_002900"
     CDS             734..1324
                     /locus_tag="ACG8KQ_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFP9105774.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1351..>1650
                     /locus_tag="ACG8KQ_002901"
     CDS             1351..>1650
                     /locus_tag="ACG8KQ_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9105775.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTR"
BASE COUNT          603 a          181 c          313 g          553 t
ORIGIN      
        1 ttaaagattg tatctattat tatagatgta ataataataa ttcattgaca cataatttta
       61 gagataatga gttcgattgt aatattgaat tagttaaaaa aattgaaggc gatttaagct
      121 atatattaga aaatgagata tttgtaaaca aaacaaggag acttttgttg ggacgctgtc
      181 gtacttgtat tcaaaaggca gttagatatg ctagttttaa taaagaattt gatttaaact
      241 cctatttgtc taagatactt atgaacgatg agttgattgc ctgtattcac tcatacccat
      301 ataaacaaaa tccaattaaa caacgaatat ttaactattt tttactaaac caatccatta
      361 ttggggttaa aacattagca tatttaagcg gaatgggtag aaataggaga ttttaatcat
      421 aaaaaactgt actatagtga atttttggag atttatattc cagttatata ttaatgattg
      481 tgatacttgt actattgtaa aaattcgtta ggtttaaata actggaaata tcggaaaatg
      541 ggcattatac atcacggaca tcccttcttt tggaaaaaat gttacgattt ttataagtaa
      601 tattgtatcc ggtggaatca acgatgaaga taacatatcc atcgttgtag attcagtagc
      661 attgaatgat gctcttgttg caagaaatcc gaataaaatt gttaagtata aattgaatta
      721 ataggagata tgaatggaaa ttagcagtaa agaatattta aaagcggttc ttcgtatgga
      781 aaagaatcta tatatagaat ctaaaggaca acacacaaaa ttactaaaaa taatgaaatc
      841 acatccggac tacaacgctt ggcggtatac tcgtcgaatg agaattacat cttattatta
      901 ttcaaagaga aagaaaaata tcatttatgc gattatgtat attgtgtctt gcagaagaat
      961 gaatagtttg ggaagaaaat gtggtataga gtgtggtgaa aatgtatttg atgaaggtgt
     1021 aagattttac catacacaag gaattgttat taatggtaac gccataatag gaaaaaattg
     1081 ttacttatat gggaataact gtattggaaa tgatggaatc gatcctaagt gtcctgtaat
     1141 tggaaataat gtacgtgtat gcgtgggtgc aaagattatt ggtaatgtaa aaatagctaa
     1201 caatgtagtt gttgcggcag gagctattgt tatcaaagat tgtctagaga atggtgcaat
     1261 tcttgcaggt gtaccggcaa aaataatagg gtatgttaat ttaacaaaag tatttttaga
     1321 ataattttta gcgaaagaag gtgaataacg atgcgtattt caataaaaag atggtcagga
     1381 gcagattgga cattgttcac gttggcattt tttgcaatct ccatagttct tgtatatact
     1441 tttaacgccc caagagcaat tttatatttg ggagatataa ttaatatctt tgtgttatta
     1501 aatgcactta gctttagacg cagaaaagga atatatgcta gcaaaaatga gatgctgata
     1561 gttttgtttt tactagtttc tatttgtagc tcagttatta atttagaaaa tataggattg
     1621 attatctggg ggatacgaaa taacactcgt
//
LOCUS       DAZKCE010000216         1617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000216 DAZKCE010000000
VERSION     DAZKCE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.223"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            486..1526
                     /locus_tag="ACG8KQ_002902"
     CDS             486..1526
                     /locus_tag="ACG8KQ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9105776.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          630 a          207 c          249 g          531 t
ORIGIN      
        1 aagttaattt taatggtagt acataattat tatatatatc tgacattggt agtacttatt
       61 gtatttttta gtagtatata atctattgtt ttctagccta aagccttgtc ttataagaat
      121 aatataatat atactactga aataaggtag tatatattac tagtaatggt agtatgtttt
      181 atatgcataa ggtagttctt aatttatttt ttgttttgac actggtagta cttaattatc
      241 tttaatttta aaactaaatt ttattcatac gtttttctgg tagtatttaa ttataaataa
      301 tatttattta aagtagttct taatgacttt aaaggtagta cataattcat tttatttctg
      361 attttaggta gtatttaatt aaaaaacagt tttaatgcgt ttgcattttt tatttatctt
      421 tgctattttc gaaaagagta ggtaatttta cataaataaa acaacgtgaa agtagaggat
      481 ctacaatgac tgattttaaa ttttttaagg cggatagagt ttacaacgaa ttattttatc
      541 aatttccaaa agtctttatt gtttctgacg aatacaaaaa aatgaaagat tcaactaaga
      601 ttgcctatat gcttttaaaa gcaagattag agatcgcaat cagcaaacgg caaatcgatg
      661 aagaaggtaa tgtttatttt acttatacga caaatgaact atgtagagta ttaaactgcc
      721 aaaaacaaaa agcgatagca atcaaaaaag agttggaatc ctttggttta ttattacaaa
      781 agcagatggg atttaacaaa cagttaggga aaaataatcc taatagacta tatctagcag
      841 aattaaaagt ctcagaaaat gatatctact tactcgaaaa atttgataga gagaataggg
      901 aaaacgttga taaatcagag ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg
      961 ctgaatccct tggggctcaa gagggtatga aaatcatacc gtgccaaaac gttgataaat
     1021 cagagggtat gaaaatcata ccagaactta ataataatat attagacact aatagacaca
     1081 atatagacac tgaaaaagac cgcctacaag atcaattgtt gttagacaat tttgagacaa
     1141 ttatgacaaa cgacagcatt gctacgtttg tccctgaacg agtattaaat ttgataaaaa
     1201 cattttcttc aagttacagt gaagctcaaa aaaccgtcca gactattcat aatgcaaaga
     1261 aaaaagctga aatagaaagt gatatttcga tagtttttga agaactcgat agttattatg
     1321 tcaatgcaga acaagaatta tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg
     1381 aaaaagtcga aaatatccag aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa
     1441 aaccaatagc tgctaaagta tcaagtgaaa aacgtttgaa ttatgcaagc tccccaagca
     1501 ctattacgaa agactggtta gagtgaaact ttactgacaa agtgattgtt gcttcatgga
     1561 gtaacattaa aattaaaaat tttttattat tctaattata ttacgaatat tacaaaa
//
LOCUS       DAZKCE010000217         1599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000217 DAZKCE010000000
VERSION     DAZKCE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.224"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1549
                     /locus_tag="ACG8KQ_002903"
     CDS             83..1549
                     /locus_tag="ACG8KQ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9105777.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          443 a          292 c          333 g          531 t
ORIGIN      
        1 ttcttaaata agttttttag ttaaattttt cggtaaagat gtataatatt ttctgaaaat
       61 ttaaagaatt ggggtataga ttatggaaaa acagcaagac aagggcttct tcgggcagcc
      121 gaagggattg tcaactttgt tcttcactga gatgtgggaa cgatttagtt attacggtat
      181 gcgtgcatta cttatctatt atatgtacga tacagttgcc aacgggggac ttggattacc
      241 acgagcaaca gcattagcta tcatgtcgat ctacggatct ttgatctaca tgtctagtat
      301 cgtcggcgga tgggtatctg accgagtgtt aggagcaaaa cgcactgtat tcttcggtgg
      361 agttttcatt atgcttggcc acatcgtctt ggctttacca tttggtgtaa gtacattgtt
      421 tgtttcgatg gcattgatta ttatcggaac cgcctttttg aaaccaaatg tttctggcat
      481 ggtcggacat ctttattcaa agactgactt gcgaagagat gcaggatttt cgatttttta
      541 catgggtgtc aacttagggg cattattagc accactaatc gttggtacaa tcggtcaaga
      601 atataactat catttagggt tctctttagc tgctattggg atgttctttg gattattaca
      661 atacatcatc caaggacgca agacattaga cggagtgggt acaacaccgc cgaatccttt
      721 gacaactgaa gaacgtaaag gattcatccg taacttgatc atcgcacttg ttgttatcgc
      781 tgcagtattc ggcggggcac aactaactgg tcatctgaca attgactttt ttgtcaatac
      841 aatcagtgta ttggggatta tcttaccgtt gtattacttt atcaaaatgc tgacttcaaa
      901 agatgtaact gctgaagaaa aaccaagagt atgggcttat atcccattat tccttgcagc
      961 aatcattttc tggtcattac aagaacaagg ttcatctatc ttagctttat ttgctagtga
     1021 acgtacacaa tctacgctat ttggtgtaac gatccaagca agctggtatc aatcattgaa
     1081 tccaattttc atctttattc tgacaccagt attcgtaacg atgtggacga aacttggtga
     1141 acgtcaacca tctacagtag tgaaattctc tcttggactt ttatttgcag gattatcgta
     1201 cttgctattg atgttgccgg gtatgcttta tggtacaagt ggaaaagtaa gtccattgtg
     1261 gttgttggga agtttcttca tcgtggttat tgctgaattg tgtctttcac cagttggttt
     1321 gtctgtgaca acaaaattag caccacgagc atttgaatca caaacaatcg ctatttggtt
     1381 cttagctgat gcttcttcac aagcaatcaa tgcacaaatt gcacgcttct acacaccggg
     1441 aacagaatct gcttacttcg gtattatcgg tgcagtagca atcgtcggtg gtatcttgct
     1501 atttactgtg aaagaaccaa tcaaaaaact aatgggctct attcattaat caatgaacat
     1561 aaaaacagga attgcttatt tgcagcagtt cctgttttt
//
LOCUS       DAZKCE010000218         1587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000218 DAZKCE010000000
VERSION     DAZKCE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.225"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..>1587
                     /locus_tag="ACG8KQ_002904"
     CDS             273..>1587
                     /locus_tag="ACG8KQ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105778.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIES"
BASE COUNT          574 a          222 c          289 g          502 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtc
//
LOCUS       DAZKCE010000219         1574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000219 DAZKCE010000000
VERSION     DAZKCE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.226"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG8KQ_002905"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG8KQ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8KQ_002906"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8KQ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          555 a          237 c          210 g          572 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttt
//
LOCUS       DAZKCE010000220         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000220 DAZKCE010000000
VERSION     DAZKCE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.227"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8KQ_002907"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8KQ_002907"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKCE010000221         1507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000221 DAZKCE010000000
VERSION     DAZKCE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.229"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..413)
                     /locus_tag="ACG8KQ_002908"
     CDS             complement(<1..413)
                     /locus_tag="ACG8KQ_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9105779.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDK"
     gene            complement(424..1092)
                     /locus_tag="ACG8KQ_002909"
     CDS             complement(424..1092)
                     /locus_tag="ACG8KQ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9105780.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1222..>1507)
                     /locus_tag="ACG8KQ_002910"
     CDS             complement(1222..>1507)
                     /locus_tag="ACG8KQ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9105781.1"
                     /translation="LILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLV
                     FNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          548 a          250 c          200 g          509 t
ORIGIN      
        1 tttttatcta atatatttga aagatctaat aattcagcta attcattgat tcttttatcc
       61 atatgctctt ttgtaaatcc aaagttttcc atcgaaaata ctaactcact cgttgtattt
      121 gttgtaaaga attgtcctct aggatcttga cttacgtagc caatattttt tgatatactt
      181 tcttgtgtta aggattgtag cgagttattt tcaaataaaa tttccccaac caattttccg
      241 tcataaagtc ttggaataag tccattcatt aactttaata gtgtggattt cccactccca
      301 gaagaaccag taatcaatgt aagactgcct ttttttattg aaaaattaag gtcaacaagc
      361 gcttgttcct taacatcctt atatgaaaaa gaaacattgt taaattcaaa caattctttc
      421 acctcaaatc aaaatactta agaaaaaaat agtaacggaa aacaggatga tatagtctaa
      481 taagctcatg tttgttttat agtaagaact tcttttcaca ggagattcta tcccctttgt
      541 tattgcatta gcggtcattt cttcagcaat catagttgca cgtataagaa cgggaactaa
      601 cagaaattcc aaatattgca gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa
      661 agattcttta atcattctta tttcagaacg ataagtcgga aagaatctga tcactactgt
      721 aatacttaac attatttttt tcggtaaatg tattttttct aaagaggata ataattcatt
      781 gctagtatag acactaacca acaaccatga gatcattacg accggcaaca ttcgaaatac
      841 aatatataag gatgtcgcaa aaaatatagt aaaaaactct atcttgatag catcaataat
      901 tttcattact aaaaacagaa ctgaataaat caagatgaaa taaatacatg tctttatttt
      961 acgaaacatc aatactaaaa ctgtagcaat aatgaaacac cccacttgaa atatatttcc
     1021 ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt
     1081 gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa
     1141 aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt
     1201 ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg
     1261 atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc
     1321 atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa
     1381 attttgattg atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt
     1441 cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa
     1501 aatcaaa
//
LOCUS       DAZKCE010000222         1505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000222 DAZKCE010000000
VERSION     DAZKCE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.230"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..749)
                     /locus_tag="ACG8KQ_002911"
     CDS             complement(<1..749)
                     /locus_tag="ACG8KQ_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HFP9105782.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEP"
     gene            complement(792..1091)
                     /locus_tag="ACG8KQ_002912"
     CDS             complement(792..1091)
                     /locus_tag="ACG8KQ_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105783.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
BASE COUNT          453 a          262 c          217 g          573 t
ORIGIN      
        1 cagggttcat ctacttgttt tttgccttct gtaccgctta aaaccgttag acttgaacga
       61 gtaaagccct tattatctgt ttgtttgaaa gaccaatctt gccattcttt gaaagaataa
      121 cggtaattag gatcaaaaaa ttctacattg tccgttcttg gtatgcgagc aataccaaaa
      181 tgattacacg ttagatcaac tggcaaagac tttccaaaat attctcggat attttgcgaa
      241 attattttgg ctgctttgac agatttaaat tctgattttg aagtcacata gactggcgtt
      301 tctaaaacaa aatatgcttg ataaccttta tcagatttga taatcatagt aggcataaaa
      361 cctaaatcaa tagcggttgt taaaatatcg cttgctgaaa tagtttcttt tgccgtgtga
      421 atatcaaaat caataaagaa ggtattgatt tgtcttaaat tgttttcaga atgtcctttc
      481 gtgtatgaac ggttttcgtc tgcatacgtt ccataacgat aaacgttggg tgtccaatgt
      541 gtaaatgtat cttgattttc ttgaatcgct tcctcggaag tcagaacaac accacgaccg
      601 ccaatcatgc ttgattttga gcgatacgca aaaatagccc ctttgctttt acctggcttg
      661 gtagtgattg agcgaatttt actattttta aatttgtact ttaacaagcc gtcatgaagc
      721 acagtttcta caacaaaagg gatattcatt cagctgttct cctttcctat aaatcctata
      781 aaataggttg tttaattaac ttggtttgct ttttcattca actgtttcaa tattgcatgt
      841 tttgaaaaag atttttttcc tttataagtc aatttttttc cactaatcga ataaattatt
      901 ttgttatttt ctattaactt atatatataa tcttccccct ccgaagaaaa atacttatct
      961 gattttgttt ctaagtagat atttctcttt tctaactctt tcttaaacgt ttctagtgta
     1021 tagatatttg ctaattttct tatctccaat aaactatttt ttatataagt tttacattca
     1081 tcatgattca tacaaactcc accttctata aatgaataca aaaaaagcaa tcaaacgatt
     1141 tccgattgat tgcttaacaa ttcttaaatt cagtagctta gatacttgaa aactctctga
     1201 tttccctata taatgatagt acggttatat accgtcttca aacaaagtta attaaataac
     1261 ttcttacgag ggaagagttc atctgactaa ctgataagcg ttggtttggc aatcttatcg
     1321 ggctatgcat ttataaaatg tcgtcaaaca ttttataaat gtgtcatggc tcttttttcg
     1381 tttctattca gttcgttgtt tcgttatatc tagtataccg cttttaaaaa aaataagcaa
     1441 cgatttcgtg cattattcac acgaagtcat tgcttttttc ttcttccatt tctaaatcca
     1501 atgtt
//
LOCUS       DAZKCE010000223         1452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000223 DAZKCE010000000
VERSION     DAZKCE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.231"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(216..1235)
                     /locus_tag="ACG8KQ_002913"
     CDS             complement(216..1235)
                     /locus_tag="ACG8KQ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9105784.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1232..>1452)
                     /locus_tag="ACG8KQ_002914"
     CDS             complement(1232..>1452)
                     /locus_tag="ACG8KQ_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9105785.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          410 a          301 c          265 g          476 t
ORIGIN      
        1 tgctctgacc aactgagcta agggtactgg ttgttgccac ataaagccat aaacaatcaa
       61 ccagtagaat gtgtggcaac aaacctgtta tcgcatagct tggagtgtga ctattatggg
      121 tgatagtgaa gatatgcgat aacatcacta ttttatcgaa tgatttttat agttgtcaat
      181 atagttatgt aatgctcctc aacgaggagc tatttttatc gtttaggaat atttaaatac
      241 caacgtttgt catggaaatc ttgcgcaccg cctttagtgt ttccctctgg atcattcgtt
      301 gcccgcatca tgacatagac tttcttatta gggaagttac gcatgttaaa agatacatga
      361 tagccaacat ttccagaagt attataagct tgatttacat ctgatctata aattccatca
      421 gctcttactc gagctaattc tttcccagta ttgtaatcca taatgaagat atactcgtat
      481 ttatagttag caatgtgcca tccagctaca tgcaaatttg cattttcgat ttctccaaac
      541 tgatcaatgt gggcgtaatt cgttccatct gtcagtgtag gattagctgc accagctcgt
      601 gttggatcaa tgacaggctt gttttcagaa gttgttggat tttcatcggt aaatccatga
      661 gctaaatcat atgctaattt ttctttactt acgcccattt cagaaagata accgtaagga
      721 tctgtatgat cgccccagat gttttgtgtt acccataaat gcgatttgat tcccggttgg
      781 ttataaggag tgtccaacgt taatggaata ccatatttca ttgctgaatc tctagccaat
      841 tcaacgtatg ccttgtagtt tttctcaaac gttgctttat catgtgtgtg ttgtaactca
      901 atctgcacag gactgttggc attagcatac gaaccagcac cgtactgtac ataaccaggt
      961 tgaccgactt gataaacaat tccgccgtct cccacaatat aagcagtata agcgctagtc
     1021 catgaacgtt gcatatactg cgcttcattg cgtcctgttg ctgtttcatt agccgtttca
     1081 tgcagtaaaa tatactgatt atttgctact tgtgagctac cttcatttgc gcccaaatta
     1141 aattcattgt tgatagtata ggcaaatccg ttaattggca ataaaaaaag agccgttaac
     1201 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
     1261 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
     1321 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
     1381 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
     1441 acgttttatt at
//
LOCUS       DAZKCE010000224         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000224 DAZKCE010000000
VERSION     DAZKCE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.232"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8KQ_002915"
     CDS             complement(150..1337)
                     /locus_tag="ACG8KQ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9105786.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKCE010000225         1370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000225 DAZKCE010000000
VERSION     DAZKCE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.233"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8KQ_002916"
     CDS             <1..792
                     /locus_tag="ACG8KQ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9105787.1"
                     /translation="QKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAY
                     NTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLR
                     EVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMV
                     GKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKE
                     IHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            782..>1370
                     /locus_tag="ACG8KQ_002917"
     CDS             782..>1370
                     /locus_tag="ACG8KQ_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9105788.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEE"
BASE COUNT          523 a          163 c          251 g          433 t
ORIGIN      
        1 cagaaatgga ttgaattgaa gttttctatt attcaaactt ggatcgattt aaaatataat
       61 gccattacta cttggaataa tattaaacag tttttcaaag atacttggaa aaatattaaa
      121 gatacggcat acaatacgtg gatttctata aaaaatacca tgattaatac atggaataac
      181 attaaggact ctttctggaa tattgttact ggaattgtta attctgctga aaatgcatgg
      241 acaaatctaa aaaatggtgt ttcaaatgct attaatcggg taaaagaaat ctttaattcg
      301 ttaagggaag tcaatttatt tgaaattggc aagaacatca ttgatggact tataaatggt
      361 gtaaaagaaa agtggaatgc tttgaaagat actatcaaag gtattgcagg taatatcaaa
      421 aattggatta aaggagcttt agatattcat tctccatcaa gatggatgcg tgatatggtt
      481 ggtaaaaata ttgttcaagg tattatcgtt ggtattgata aagagcaaag taagttagat
      541 caaaccatga caaatttagt aaaaacaccg tctgttcaac ctgtagtaaa tggaataagt
      601 cacctaccaa ttacacaaac aaagcaaaat atatctgcta gtgaaacaaa agaaattcat
      661 ttacatttaa atgtttatgg agatttacct gattcaatgg ttcggaagat taccacaaaa
      721 attaaaacag aattgacaag acaaatgaaa cgagatgctg gtgcagtagg aggaacatta
      781 tatgcaactt aagagaggac aattttttat taatcaacat tattcttctg aatttaatgt
      841 gtatattcaa aacaggcctg cttctgtttc agctagtcga gtaattgaat tgagagagcg
      901 agaaggaaat gattctatca ttattgataa aggctattac aaaaatgtga ctagaaagat
      961 tgagtgttac tataaagcac cttcaattga tatggtgcag gagtgggaag atcgaatcac
     1021 tgaatggtta gatatgtcgt cttatagtga ttttattctt tactatgatg aacaatatat
     1081 ttatcaagct gtagtaatag aaggacctga atttaaagga acaagaaaaa caggaaatat
     1141 tgtccctttt gaatttacag tgagtattcg accatttaag gaaaattaca atggtagatt
     1201 tactattcga caaacagaaa cttttgaaat ttataaccca gagaagtatt cttcaaaacc
     1261 gcttattaaa ttgagtggtt ctggagatgc ttctttttat attaataagg ataaatatga
     1321 tttgaaatcg ttggacagag aattatttat agattctaaa ttagaagagg
//
LOCUS       DAZKCE010000226         1360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000226 DAZKCE010000000
VERSION     DAZKCE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.234"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1360
                     /locus_tag="ACG8KQ_002918"
     CDS             82..>1360
                     /locus_tag="ACG8KQ_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9105789.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          463 a          262 c          257 g          378 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DAZKCE010000227         1339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000227 DAZKCE010000000
VERSION     DAZKCE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.235"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..446)
                     /locus_tag="ACG8KQ_002919"
     CDS             complement(147..446)
                     /locus_tag="ACG8KQ_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9105790.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(452..682)
                     /locus_tag="ACG8KQ_002920"
     CDS             complement(452..682)
                     /locus_tag="ACG8KQ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105791.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(941..1054)
                     /locus_tag="ACG8KQ_002921"
                     /pseudo
     CDS             complement(941..1054)
                     /locus_tag="ACG8KQ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          479 a          216 c          200 g          444 t
ORIGIN      
        1 atgttagaga aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat
       61 atggcgctga tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca
      121 agcgagtttg tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta
      181 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacatc agtcacgata
      241 tcaaacaaaa aatatacgtc tttcttcatt ctagttttcg cagcagggat tttaaagtag
      301 ttcttattag aatagtagag attaattaat aagctatctt cgattgctaa aaagaaaact
      361 tctgaatccc acaccttata aaaatctttg acaaatctat tcgaaggatc aaatttaaac
      421 cataattgcg tctcattaaa aagcataacc attactccga tctattttta aacttagtct
      481 ctacctctaa tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg
      541 cagttacagg ttgtttattt tcggaaaaag ctaatatagc taaaattgaa acatccatct
      601 ggaatttgtc tttttcgttg ctttgctcat aaaaatctgc atattcatca ctaatatttt
      661 ttctaataaa ttcttccatc ataaagatca cctcgaaaag agtatacgaa caaacgttct
      721 ttttgtaaag gtggaattta ttatactaaa taaataggtg aacaagtact tgtgccaaat
      781 tatgtgccaa aaaaaatcga atttaataaa acacaaacaa aaagaaatcc tattatgata
      841 acatttctta taataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa
      901 ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt
      961 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
     1021 aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa
     1081 aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat
     1141 agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc
     1201 gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga
     1261 gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc
     1321 aaagtaccat ataaaaaaa
//
LOCUS       DAZKCE010000228         1331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000228 DAZKCE010000000
VERSION     DAZKCE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.236"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8KQ_002922"
     CDS             288..695
                     /locus_tag="ACG8KQ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105792.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8KQ_002923"
     CDS             709..1110
                     /locus_tag="ACG8KQ_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105793.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..>1331
                     /locus_tag="ACG8KQ_002924"
     CDS             1112..>1331
                     /locus_tag="ACG8KQ_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105794.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTV"
BASE COUNT          500 a          163 c          229 g          439 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt a
//
LOCUS       DAZKCE010000229         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000229 DAZKCE010000000
VERSION     DAZKCE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.237"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8KQ_002925"
     CDS             <1..1244
                     /locus_tag="ACG8KQ_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9105795.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKCE010000230         1279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000230 DAZKCE010000000
VERSION     DAZKCE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.238"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1042
                     /locus_tag="ACG8KQ_002926"
     CDS             35..1042
                     /locus_tag="ACG8KQ_002926"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFP9105796.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1054..>1279
                     /locus_tag="ACG8KQ_002927"
     CDS             1054..>1279
                     /locus_tag="ACG8KQ_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFP9105797.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKL"
BASE COUNT          507 a          163 c          243 g          366 t
ORIGIN      
        1 tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa caatattagt
       61 aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa aatatgaacc
      121 aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc aacaagagct
      181 ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc aaaggattga
      241 ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac acgttccagc
      301 aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc aaaatgtaat
      361 agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag ataaagtagt
      421 gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa taatagctgc
      481 caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg gaaatgtcat
      541 ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa ataaaaaaca ataaagatat
      601 tactttgaca gatgttgata tgacacgatt tatgatgtca ataccggatg ctgcaagatt
      661 agttctgtct gctgctatga attcaaaatt tggagaaaca tatgtcttaa aaatgccggc
      721 aatcaatatt ttaactttag ctcaatcatt aataaaaata tataatgata cacatgatga
      781 aaaatataga ggtgaaataa aagttgtagg tatcaggcca ggagaaaaat tatatgaaga
      841 gctaatgact acagacgaaa ttgaacgctc atatgaaaat gataaaatgt atatcattcc
      901 accacactat catgctactg acgaactgta caaagattat gaaaaaatta aaatcactgc
      961 atactcttca gatgcaacag aattaatgag tggacaggaa tttattagaa tcgcaaaaga
     1021 gaataatgtc gtgacttggt aaggagtttt atgatgaaat cagaagtata tgtaattgca
     1081 gaaattggtg taaaccataa tggaagtcta gatttagctt tagaaagtat tgataaagct
     1141 aaagaatgtg gagctaacgc ggttaaattt caaacattta aaacgaataa gctagtttcc
     1201 aaaaaggctc caatggcaaa atatcagaag gataatctta aagaggacag ttctcaattt
     1261 gaaatgctaa aaaaattag
//
LOCUS       DAZKCE010000231         1200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000231 DAZKCE010000000
VERSION     DAZKCE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.239"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8KQ_002928"
     CDS             <1..969
                     /locus_tag="ACG8KQ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP9105798.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          216 c          242 g          339 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
//
LOCUS       DAZKCE010000232         1198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000232 DAZKCE010000000
VERSION     DAZKCE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.240"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..840
                     /locus_tag="ACG8KQ_002929"
     CDS             <1..840
                     /locus_tag="ACG8KQ_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9105799.1"
                     /translation="VLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLF
                     LLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVE
                     VIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYG
                     NSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLL
                     KTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLK
                     KEN"
     gene            842..>1198
                     /locus_tag="ACG8KQ_002930"
     CDS             842..>1198
                     /locus_tag="ACG8KQ_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105800.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNY"
BASE COUNT          403 a          144 c          195 g          456 t
ORIGIN      
        1 gtattaattt caaaaataac aaaaacgatt cgtgtttttt atatgatgaa ttttatcgta
       61 caaatatttt atattacact ttcttcgtct agaggtgccc aggtagtaat ttgttctttt
      121 ttagtattat ttcttttgtt gtttgccaat tcaaaattta gaaagagcat tagagaagta
      181 ctacctgtaa aaatcaaatg gatggtaagc atattattag ttctattggt atatttttct
      241 tttggtacta attatgctaa ggatttttta gcaacaattc ctgtatcgat tgatagaaaa
      301 gtagaagtta tagagtcaaa tgaatcagat aaaactccac ttccagaaaa agaagtaata
      361 ttacagcatt ctagtgataa ctccgaaatt tcagctggtc gttttacgat ttggaaagct
      421 gcgcttaaag ttataagacc aactttgtta tttggttatg gagagaccga tttctatggg
      481 aatagcgatg caaaattttt gaaaaaagta gatcttgata ctacagatgt gaatgaacta
      541 aaaagagcac atggaaacat gcataatgga tttcttcaag tacttgtatc aagtggtata
      601 attgctttta tatgtatcta tgctttctat gtgttaaata tctttagttt actcaaaaca
      661 ttttttagaa gtaaagtgaa ttatgcgttt cttggtattg ttttaatttt tatactatct
      721 atttttatag atgaaatggt ggaggctcat gtacttttca ataagcgcga tgtcatttca
      781 attgttttct ggtattattt gggaagcatt tcttacttgt atttaaaaaa ggagaattaa
      841 tatgaagaca gctttcattg cttggactcc tttacatttg attaatatat taaatgttta
      901 tcaaaatttt taccgagaat caaaaggaga tttatttatt tattctgaat ttgaaaacgc
      961 tagaagatat agtgagcgta taaaagaatt agggctattt aatgatgttg tttttgtgaa
     1021 tcaccggaaa atcggttctt cctttaaacg taaaattaac ttgttaacta accataatca
     1081 atttttctcg gatgatttat ccgtttatga tgaaattttt atacaggggg aaaattattt
     1141 cacaaaaata cttttctcca atgtttacaa aaaaaatcca gatgtaaaac taaattat
//
LOCUS       DAZKCE010000233         1177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000233 DAZKCE010000000
VERSION     DAZKCE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.241"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG8KQ_002931"
     tRNA            complement(21..94)
                     /locus_tag="ACG8KQ_002931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(649..963)
                     /locus_tag="ACG8KQ_002932"
     CDS             complement(649..963)
                     /locus_tag="ACG8KQ_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105801.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          443 a          186 c          185 g          363 t
ORIGIN      
        1 gattcaattg gctacattaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctgaccaac tgagctaagg gtagtggttg ttgccacata aagccataaa
      121 caatcaacca gtagaatgcg tggcaacaaa cctgttatcg catatcttgg agtgtgacta
      181 tttatgagtg atagtgaaga tatgcgtctc catatatgta tggttgagac agataaacat
      241 gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg tcaaggagag aaaagtagag
      301 aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca tttagaaatc
      361 cccaatacgt attcaggaaa atctatttta aggtttacaa aaagaacttt tgttcgtaca
      421 ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg gtttaagcat
      481 gttccttcaa ataagttttt taattataaa ctctagagaa attaattcat cctaacataa
      541 aataattgtt cactcaaaag tttttctaga acaattaaaa cagcaggctt cacattcgct
      601 atacatttgt aagaagtaat aattcctgta ctaaaaggca taattgcttt aattattttt
      661 tgacttctct tcaaacacaa gaagtagttg tgtaataatt gcagaaataa ggattccaat
      721 aatagatata aaatttgaca tggaaacgtc tataacatta aaagaataaa gtgttgaaga
      781 aagtaaactg attgaaataa cccctccgta aaaaatagtc tgtgaatata cccacttttt
      841 ttcagaagac gtacttcgtt tggttctaaa acctatccct gcatttctac tcttatcaaa
      901 aaaccaaata tttagaagta ataatattgc tattattaag ataaaattac tgtaaaaatc
      961 caaaagctat ccgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
     1021 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
     1081 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
     1141 tattccgttc gcacttagaa aaagcgataa acacact
//
LOCUS       DAZKCE010000234         1169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000234 DAZKCE010000000
VERSION     DAZKCE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.242"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..479
                     /locus_tag="ACG8KQ_002933"
                     /pseudo
     CDS             165..479
                     /locus_tag="ACG8KQ_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(784..>1169)
                     /locus_tag="ACG8KQ_002934"
     CDS             complement(784..>1169)
                     /locus_tag="ACG8KQ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFP9105802.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          421 a          163 c          212 g          373 t
ORIGIN      
        1 aatcttcttt atagagaatt gggctatttt taatggcaaa gaggggaaga agatgtttca
       61 aaacaaagga caacgctatg ttgtaaaagg agtaaaggaa tcttactgga atcaaaaatc
      121 tttattgaga aaattatctt tgaatttaag cacaaagaaa tgaaggtaca ctagatagca
      181 atgaagatat ctttgtcatt aaacgtcgct attttacaca agtaagtgat gatttgattc
      241 atgtcttttc caatgagtgg tttgtttctg agaaaaaatt acatgcttct aatctgcaat
      301 atatgccagg tgaagatcca attcctaata tgaaagcaat tatcaacagt aaggattacg
      361 aaggatataa agccaaacat ccagaagcga aaccatttaa atacccacaa gaaatgaagc
      421 gagcatggcg taaaatgtta gatgatgagc tgattcccct tgaaaatgaa ttgaggtaaa
      481 cgagaagaat tcaataaact ggatgataaa aatataatat gactataaat tggttacagc
      541 ttcaacctat aagttgtaat aaattagcct aaaagtaatt tattaatgat gaggtgaatt
      601 tgattggtac aacaagatat atcacttaga gagggagaag cagcactaca aactactttt
      661 caataagtag gaattataga aattttactc aaaaaatctt cagtaaacaa tttggctaaa
      721 taagattcag ttgttatacg agaaggttac ggttcttctt aactaagctt agtatgtttt
      781 tattcaaggc atgattttct ctatgtgatc attcagtttc tgtaagagtt ctaataattc
      841 ttttttggag tacttgtaat tgctttctac ccaccgattg agaacggata gacaaccttg
      901 gacgtggtac gtagtaaaga atctcatttc ttctgtgata atcgttttct tgtcttcata
      961 tagccatatg ttcagatagt tttcttcaat aacttgttta actttcttct gaaacgaaat
     1021 atcaccatgg ttgaggataa ataagcgata taaaaaagta tttgcgtaaa catagtcaat
     1081 caagcgagtg tatagttcct catagctatg ggctggatca taagagagaa tttcatttag
     1141 atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKCE010000235         1132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000235 DAZKCE010000000
VERSION     DAZKCE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.243"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..548)
                     /locus_tag="ACG8KQ_002935"
     CDS             complement(<1..548)
                     /locus_tag="ACG8KQ_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFP9105803.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGA"
     gene            complement(541..>1132)
                     /locus_tag="ACG8KQ_002936"
     CDS             complement(541..>1132)
                     /locus_tag="ACG8KQ_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9105804.1"
                     /translation="DQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLR
                     MIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFF
                     HTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQ
                     IKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          409 a          173 c          135 g          415 t
ORIGIN      
        1 gctccaacat ttggacttgt atagacacca aataaccgat gttcaataaa accttgtctt
       61 gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa tcaaaaaaat aaattgatag
      121 atagatatta gcgcagctaa aaaaattatg ataatgaaaa cattattaaa atacaccatt
      181 tttttctttt tttcctcttc actttgtgaa aagtctgcag gaaaaattac tatcaaagta
      241 atcagcaaat atccaaaatt ataaatagat ggaattaaaa aattttctct gttaatgatt
      301 atagttaaga taaaactaat cattgctcca atcagtagcc aagaaaaacg cattttaaaa
      361 aaatttttct ttataaaaac ctcatacaaa actataattc caccccaagc aaaagctatt
      421 ttcatcggga tattagttac attaaaagta ctaggcataa aataaagcag acaaatgatt
      481 aaatttatta ctcgaaacgc atctcgactt aataaactct tctctaaaac aggtactatt
      541 ctattcatct atatttccaa ctttcaaaat ttctttatca attctattta atagtttatc
      601 agagtaatcc gatttaactt ttcttacaaa taatttttca ctttctttta tttgatctat
      661 atccctttca tcaaaccatt tgttcatgga aggatcaata atatgaatat ttgctaattt
      721 cgaagtttgt aaagtagttt cttcaatatt tttcaattca agtggtaaat ctggtcctcc
      781 aataacattt ttatttttga acgaggaatt aaaaaaaagt gtatgaaaaa atttttcatc
      841 tggggcaaac atacctattt taaaattcag gtagtcttgt tgattttgtt catacatttg
      901 tattaattct gttaggcatt ctttcgacaa agcccaccat tgagaaccat gatacaaatc
      961 aaatttatgg tgtctaattt ttgggggaag tttaatcatc ctcagacaac gattaattat
     1021 agctctaaaa attttaaatg cagttgtgtt attcctatta attctaggat aatcatgttt
     1081 ttgatataca tctatatgtt tataataaaa ttctttatgt ttaatttgat ct
//
LOCUS       DAZKCE010000236         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000236 DAZKCE010000000
VERSION     DAZKCE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.245"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8KQ_002937"
     rRNA            <1..>1073
                     /locus_tag="ACG8KQ_002937"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKCE010000237         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000237 DAZKCE010000000
VERSION     DAZKCE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.247"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..433)
                     /locus_tag="ACG8KQ_002938"
     CDS             complement(<1..433)
                     /locus_tag="ACG8KQ_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105805.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNN"
     gene            complement(440..988)
                     /locus_tag="ACG8KQ_002939"
     CDS             complement(440..988)
                     /locus_tag="ACG8KQ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFP9105806.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          359 a          203 c          103 g          370 t
ORIGIN      
        1 aattattttt taatagaaac tgaaaattaa aactagaaaa agtagtttta ccgaatgtaa
       61 gagcatcatc accatcaata tagaaataca ttcttttcca gttacattta atttttaaac
      121 aaaattctcc ccaaaaagga actttttcta gtgaaaactt ggtaatctct ttgtaattaa
      181 ttcttgttaa agaataatta aacacttcat ttttacttgt tattttttca ttgactataa
      241 ctagttcatc tggtaaaaaa attaaccaac aaacatcgac aaacagtaaa tgatataatc
      301 catttccacc tccaagcata ttcaatactg gtgaacatgc cttagaaaca cgaatatagt
      361 tatggtttaa atagatattt tctttaaaca ttgcttgttt aaaagccata tgctcttgtt
      421 ctaaactttt cataaacttt tactctaatg cacagctagc agcagctgct gcaattcctg
      481 ctacaatatt taccttagct aactctttag ctgcagcttt tcctaattgt ttttctgcaa
      541 tttttaataa taaatccgca gctttttcaa aattaaaagc ttttaatgct tcaataaaat
      601 ctccctgcgt acctatagcc caagccactc ctcctccagg aagagaatta atggctacac
      661 atccagcata tcgtttccaa tcaactttct ccattctagg aacaataata tgttcataaa
      721 agactcttgc tgctcttcct tgttcgttat cttgactgat tcgttcacga atagcaccaa
      781 aatcctttat atggtataca ccatgttcgt ctaaaaaacc aactgtatca aaataaaaag
      841 ttaattcatc tactaattta ttctttattt ctaaatcaac tgtattcact ttttctgtgg
      901 catacacact tgttccagta ctaacaaaag tacttaaacc taataagaaa acacctaaaa
      961 caccaaatat tttaattatt cttttcataa tttctcctcc ataaaaaata agttactttt
     1021 taaatatctt gttta
//
LOCUS       DAZKCE010000238         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000238 DAZKCE010000000
VERSION     DAZKCE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.248"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG8KQ_002940"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG8KQ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKCE010000239         1000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000239 DAZKCE010000000
VERSION     DAZKCE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.249"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8KQ_002941"
     CDS             complement(<1..434)
                     /locus_tag="ACG8KQ_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105807.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8KQ_002942"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8KQ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9105808.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          304 a          172 c          163 g          361 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatggaac
      901 aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg taaccctcat
      961 accgaacggc attcatcacc cacctataga ggatgggcga
//
LOCUS       DAZKCE010000240          991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000240 DAZKCE010000000
VERSION     DAZKCE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.250"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG8KQ_002943"
     tRNA            complement(21..94)
                     /locus_tag="ACG8KQ_002943"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG8KQ_002944"
     tRNA            complement(108..181)
                     /locus_tag="ACG8KQ_002944"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG8KQ_002945"
     tRNA            complement(209..281)
                     /locus_tag="ACG8KQ_002945"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG8KQ_002946"
     tRNA            complement(301..374)
                     /locus_tag="ACG8KQ_002946"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACG8KQ_002947"
     tRNA            complement(384..457)
                     /locus_tag="ACG8KQ_002947"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACG8KQ_002948"
     tRNA            complement(468..553)
                     /locus_tag="ACG8KQ_002948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACG8KQ_002949"
     tRNA            complement(570..641)
                     /locus_tag="ACG8KQ_002949"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACG8KQ_002950"
     tRNA            complement(653..725)
                     /locus_tag="ACG8KQ_002950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACG8KQ_002951"
     tRNA            complement(743..824)
                     /locus_tag="ACG8KQ_002951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACG8KQ_002952"
     tRNA            complement(832..904)
                     /locus_tag="ACG8KQ_002952"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACG8KQ_002953"
     tRNA            complement(908..980)
                     /locus_tag="ACG8KQ_002953"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAZKCE010000241          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000241 DAZKCE010000000
VERSION     DAZKCE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.251"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG8KQ_002954"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG8KQ_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          351 a          175 c          197 g          265 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaa
//
LOCUS       DAZKCE010000242          978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000242 DAZKCE010000000
VERSION     DAZKCE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.252"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..695)
                     /locus_tag="ACG8KQ_002955"
     CDS             complement(<1..695)
                     /locus_tag="ACG8KQ_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9105809.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNL"
     gene            complement(771..917)
                     /locus_tag="ACG8KQ_002956"
     CDS             complement(771..917)
                     /locus_tag="ACG8KQ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFP9105810.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          376 a          155 c          105 g          342 t
ORIGIN      
        1 aataaatttt tatagtcatg tctaaatctg gcaaattttt cttgatcttt ttccagtgaa
       61 tcagtatatt tttttagata aaccaattct tttcttttca attgctgttc atattgttct
      121 cgttgcctaa tagttattct aataaataaa aatagaacaa caaacatttg aatgattaaa
      181 aatattaaga caccaaacac aaaatgatca aatacttgat attgatgagc aacataagaa
      241 acaaataaac caattgtaag taaataaatc atgaatatag cagtaagttt agaactggac
      301 tgttcaacaa tcctatttat agctaatttt ctatataacc aaataaacat cagcaaaaac
      361 aaagaatcaa gcattatctg gaatactaca taaccatacc cttttatccc ttcaaatgaa
      421 aacatatacg tcataaccat ggaagtaata atagatctga aatcatcaat aagcatacat
      481 aatagtaatt tatttaaaag cactatatta gttttcttct tttcctttaa tataaaataa
      541 cttccgatta taggtatagc tgcggaaaca tcagtaaata attcaacaaa aaaagtgagt
      601 ggtatcaata aacatacaaa aagatatttt cttttgttaa gatcagtatt agtcatataa
      661 aataagagaa aaaagaaaaa gtttcccacg aacattctta atagataagc ttttgtatcg
      721 atagtaatca agagatcccc tcctgatttt aggatatcat aaaaaaaatt ttagttacac
      781 tttttaaata atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt
      841 gttccagcac ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt
      901 ctattttttt cttccattac gattccccct acaaactata aatactttta tgtcactgat
      961 tcaatatacc tatttttc
//
LOCUS       DAZKCE010000243          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000243 DAZKCE010000000
VERSION     DAZKCE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.253"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8KQ_002957"
     CDS             <1..459
                     /locus_tag="ACG8KQ_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105811.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8KQ_002958"
     CDS             473..904
                     /locus_tag="ACG8KQ_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105812.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKCE010000244          956 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000244 DAZKCE010000000
VERSION     DAZKCE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.254"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..>956
                     /locus_tag="ACG8KQ_002959"
     CDS             44..>956
                     /locus_tag="ACG8KQ_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105813.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGIL"
BASE COUNT          316 a          120 c          135 g          385 t
ORIGIN      
        1 tgtttaaaaa aaataaatga tactatatgt aatataaaaa aacatgaggt ggtatatgaa
       61 tttgaaaaaa attttttctg taaaaatatt tatagtttat gcatttgtta gtttattata
      121 tttatttcca gcatttaaaa acatggaaat atttggcttt tatgatacgg catttcacat
      181 taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg aaacgtttag
      241 atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc ctttattttt
      301 attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata ttgttacgct
      361 cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac atgttactag
      421 ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa tatttcttcg
      481 aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac tcgggtttat
      541 aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca tgactttatt
      601 aatttacact catgttcttt ctgtagctat gactatggta atgattataa ttgctttaat
      661 tattcaattt tatcggaaaa agaaaataga aatcttctta attattaaat taataaaagc
      721 tgctatagtt actttacttc tttcactagc gtttataggt cctatgattg agcagacatt
      781 gtatcaagat ctgaatagac cttatgtgga tatattacaa aaaagagcaa tttatctggg
      841 taaagagttt ttgattggaa gtatagattc agaattacta tcatttggta ttggcttaac
      901 tttgcttatt tgtttaatta tactaacatt taactttaaa aaattaggaa tcttat
//
LOCUS       DAZKCE010000245          953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000245 DAZKCE010000000
VERSION     DAZKCE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.255"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..>953)
                     /locus_tag="ACG8KQ_002960"
     CDS             complement(80..>953)
                     /locus_tag="ACG8KQ_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105814.1"
                     /translation="EKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLG
                     EKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQL
                     IRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQD
                     KFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEA
                     GKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDK
                     QIDDFPEEIIDRIE"
BASE COUNT          307 a          161 c          145 g          340 t
ORIGIN      
        1 taaatggaaa gttttgtctc gcttaatttg acaaataata tttttagaaa aagcagtcgg
       61 actttctaaa agaagttcac tattcgatcc tgtcgatgat ttcttcagga aaatcgtcta
      121 tttgcttgtc aattttgctg tctttgtatt tctcttcttg gatagtcaac aaaatctttg
      181 taagattccc ccagtctact gctcttggcg gatcgagcca aaccactact tgagatgcac
      241 tttcttcata tagattattc ctattcgtga ttataacatc cgggaactca ggctttcctg
      301 cctctatcac ttccgtgttc agattaatgt acaagcttaa atttttcttg ataaattgat
      361 tataggcagc accataagaa aaatcgatga tgatctttac tggttcagaa atcagttgaa
      421 tgggtatgtg attgatcaat aataataaat aactgaaaaa aagaaaaaat ttatcttgaa
      481 gcaactcttt gcgttttgac gttagatcat ttatatactc tatcaaatag caataaaatg
      541 ctggatagtt ttctctaaaa aaagaaacat caagtggaaa ttcatcagct ttattaatag
      601 tgtaatgcaa aagtttaaaa tgtattttgg ttaactctct tcggatcaat tggctcgtct
      661 cttctcgcaa cagattaaac tgattttcaa aactttcaat gaatcgttca ttcaaccatt
      721 cgattgtttc atcttcattt tttctcaagt atttttgatc caatttatca tgaagataaa
      781 gccaagagag tgtcttattg ataaaaataa cggatttttc tcctaatact ccttttaagt
      841 ggtaaaatcc tatatccgaa aaaaatagcg actgattgct agcaatatct aaagaatcag
      901 cttgttcagc caacaggttg attgctaaaa agtggaataa tttctttttt tca
//
LOCUS       DAZKCE010000246          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000246 DAZKCE010000000
VERSION     DAZKCE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.256"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG8KQ_002961"
     CDS             <1..808
                     /locus_tag="ACG8KQ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP9105815.1"
                     /translation="VHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKI
                     MLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          342 a          111 c          161 g          317 t
ORIGIN      
        1 agttcattat ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata
       61 tacagaaaat ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa
      121 ttacgatcca gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa
      181 tggagatatt cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag
      241 tgttagtgga gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt
      301 agcacaaacg tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc
      361 tttgatacaa gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc
      421 tgggattgtt tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt
      481 aatttcttct gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga
      541 aatagctaat tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat
      601 gcatattacg atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt
      661 gaaatcaaac catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca
      721 cttttcgaga gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt
      781 agtggacaat ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt
      841 tacgtagttt ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt
      901 ctgatttgag ttgtttcgta gaatctgagc g
//
LOCUS       DAZKCE010000247          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000247 DAZKCE010000000
VERSION     DAZKCE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.257"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8KQ_002962"
     CDS             <1..355
                     /locus_tag="ACG8KQ_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105816.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8KQ_002963"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8KQ_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKCE010000248          898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000248 DAZKCE010000000
VERSION     DAZKCE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.258"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG8KQ_002964"
     CDS             256..879
                     /locus_tag="ACG8KQ_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9105817.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaaga
//
LOCUS       DAZKCE010000249          892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000249 DAZKCE010000000
VERSION     DAZKCE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.259"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>892
                     /gene="bsh"
                     /locus_tag="ACG8KQ_002965"
     CDS             4..>892
                     /gene="bsh"
                     /locus_tag="ACG8KQ_002965"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9105818.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITA"
BASE COUNT          302 a          122 c          163 g          305 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aa
//
LOCUS       DAZKCE010000250          890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000250 DAZKCE010000000
VERSION     DAZKCE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.260"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..561
                     /locus_tag="ACG8KQ_002966"
     CDS             88..561
                     /locus_tag="ACG8KQ_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9105819.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            570..797
                     /locus_tag="ACG8KQ_002967"
     CDS             570..797
                     /locus_tag="ACG8KQ_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105820.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          299 a          129 c          155 g          307 t
ORIGIN      
        1 atttttattg tttttcgata aaaaatagtt gttttacaat aattattctg ctatatttta
       61 gctatcaaag ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc
      121 gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct
      181 acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac
      241 tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc
      301 gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc
      361 attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat
      421 ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca
      481 gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca
      541 gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat
      601 tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa
      661 acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa
      721 tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata
      781 ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat
      841 agatgtgtga gacttttaga agttatttac gtctattatt cagatttttt
//
LOCUS       DAZKCE010000251          845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000251 DAZKCE010000000
VERSION     DAZKCE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.262"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..104)
                     /locus_tag="ACG8KQ_002968"
     tRNA            complement(32..104)
                     /locus_tag="ACG8KQ_002968"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:His,seq:gtg)
     gene            complement(107..180)
                     /locus_tag="ACG8KQ_002969"
     tRNA            complement(107..180)
                     /locus_tag="ACG8KQ_002969"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Trp,seq:cca)
     gene            complement(187..267)
                     /locus_tag="ACG8KQ_002970"
     tRNA            complement(187..267)
                     /locus_tag="ACG8KQ_002970"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Tyr,seq:gta)
     gene            complement(269..344)
                     /locus_tag="ACG8KQ_002971"
     tRNA            complement(269..344)
                     /locus_tag="ACG8KQ_002971"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Phe,seq:gaa)
     gene            complement(380..452)
                     /locus_tag="ACG8KQ_002972"
     tRNA            complement(380..452)
                     /locus_tag="ACG8KQ_002972"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Asp,seq:gtc)
     gene            complement(462..534)
                     /locus_tag="ACG8KQ_002973"
     tRNA            complement(462..534)
                     /locus_tag="ACG8KQ_002973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Val,seq:tac)
     gene            complement(550..621)
                     /locus_tag="ACG8KQ_002974"
     tRNA            complement(550..621)
                     /locus_tag="ACG8KQ_002974"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(586..588),aa:Glu,seq:ttc)
     gene            complement(636..725)
                     /locus_tag="ACG8KQ_002975"
     tRNA            complement(636..725)
                     /locus_tag="ACG8KQ_002975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(689..691),aa:Ser,seq:gga)
     gene            complement(765..838)
                     /locus_tag="ACG8KQ_002976"
     tRNA            complement(765..838)
                     /locus_tag="ACG8KQ_002976"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Asn,seq:gtt)
BASE COUNT          237 a          232 c          192 g          184 t
ORIGIN      
        1 taccgcttgg ctatacccca agaataaaaa gggcgaatga tgggaatcga acccacgaat
       61 gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatggc aggggcagca
      121 ggaattgaac ccacactaac ggttttggag accgctgttc tacctttaaa ctatgcccct
      181 ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt
      241 cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg acagaatcga actgccgaca
      301 cacggagctt caatccgttg ctctaccaac tgagctaccg cgccatattt taaaattatt
      361 aattgaataa caagaacgac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca
      421 ggcaggcatg ttaaccccta caccacggaa ccagttcatt atggaggtta acgggatcga
      481 accgctgacc ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccaacatt
      541 caattaaaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct
      601 taaccgcttg accaacgggc caggtaaatt tgctacggag agtaagggat tcgaaccctt
      661 gagacagttt ggcaccatct acatgatttc caatcatgct ccttcggcct ctcggacaac
      721 tctccagagt gaaagaagct caatactctt tctcaaaatg agaactccgg cagtaggact
      781 cgaacctacg acatcatgat taacagtcat gcgctactac caactgagct atgccggaat
      841 aacat
//
LOCUS       DAZKCE010000252          829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000252 DAZKCE010000000
VERSION     DAZKCE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.263"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..785
                     /locus_tag="ACG8KQ_002977"
     CDS             <1..785
                     /locus_tag="ACG8KQ_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9105821.1"
                     /translation="MMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFKNK
                     EILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSL
                     TKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYI
                     GKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFD
                     LNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          370 a           56 c          118 g          285 t
ORIGIN      
        1 aaatgatgaa acttgataaa aaaatgagtt taaaagttag ctctaatgat gaagttggag
       61 aattaaaaca acaaattaat gatttgtatt ctactttatt aagaacaatt gacgatttgg
      121 aatttaaaaa taaagaaatt ttaaaattag aaaagttaaa atatgacttt tttaaaggtg
      181 catcccatga acttaaaacc cctcttgcta gtcttaaaat aatactggaa aacatgaaat
      241 acaatattgg aaaatataaa gaaagagata tttatattaa tgaatgtatt gaaatcgttg
      301 atagtttaac aaaaaatatt tctcaaatat tatcagttca ttctatagaa aatttgaata
      361 atgatgaaga atatttgaaa ataaatgata cattagaaga catattaaaa aagtatgaaa
      421 tattagcaaa tcaaaagaaa ataactgtca ataattatat attagacgag aatgtttata
      481 taggaaaaac agctttaaag attattctat ctaatttgat tagtaatgcg gtaaaatata
      541 ctgatgtaaa tggggtaatt aatattggga tagttaatga ttggttatat atagagaatt
      601 catacggtaa caataaaatt tcgaatatgg ataaaatatt tgatgttaaa tttgatttaa
      661 ataaggaaaa tagtaatgga ttaggtttat atattgtaag taatattctg aataattaca
      721 atatagaata taaagtattg caggatgaag aattttttat ctttaaaata aaaatatttt
      781 cttaattaag atataatttt gcaatagtta ttgaaaaata gagacttat
//
LOCUS       DAZKCE010000253          824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000253 DAZKCE010000000
VERSION     DAZKCE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.264"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG8KQ_002978"
     CDS             complement(<1..153)
                     /locus_tag="ACG8KQ_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9105822.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACG8KQ_002979"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACG8KQ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          152 c          158 g          255 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ctct
//
LOCUS       DAZKCE010000254          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000254 DAZKCE010000000
VERSION     DAZKCE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.265"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8KQ_002980"
     CDS             <1..672
                     /locus_tag="ACG8KQ_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9105823.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKCE010000255          809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000255 DAZKCE010000000
VERSION     DAZKCE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.266"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>809
                     /locus_tag="ACG8KQ_002981"
     CDS             <1..>809
                     /locus_tag="ACG8KQ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFP9105824.1"
                     /translation="TALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVI
                     IGRKERPVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL"
BASE COUNT          279 a          150 c          145 g          235 t
ORIGIN      
        1 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
       61 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
      121 gcctgacgtc attatcgggc gaaaagaaag gcctgtttca tgcggtatga gaacacttta
      181 tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa
      241 acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg
      301 acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt
      361 tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat
      421 cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt
      481 ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc
      541 taactggaaa gccattagta accaacatga tattgatata tattttgcgg accctggaac
      601 accttctcaa cgcccattaa acgaaaattc taacgggatt ctgcgtcgta atggactgcc
      661 gaaatcaata gattttagag aagtgaatca gacatttatt tccagtgtca gcaatcaacg
      721 caatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat ttttgagcta
      781 tgtacaagaa gcattttatt ctagcttaa
//
LOCUS       DAZKCE010000256          807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000256 DAZKCE010000000
VERSION     DAZKCE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.267"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8KQ_002982"
     CDS             134..307
                     /locus_tag="ACG8KQ_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105825.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          272 a          130 c          102 g          303 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaag
//
LOCUS       DAZKCE010000257          773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000257 DAZKCE010000000
VERSION     DAZKCE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.268"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8KQ_002983"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8KQ_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9105826.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACG8KQ_002984"
     CDS             complement(481..675)
                     /locus_tag="ACG8KQ_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105827.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          229 a          162 c          134 g          248 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DAZKCE010000258          722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000258 DAZKCE010000000
VERSION     DAZKCE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.269"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..188
                     /locus_tag="ACG8KQ_002985"
     CDS             <1..188
                     /locus_tag="ACG8KQ_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105828.1"
                     /translation="IEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNN
                     YQFGNLSYEMISKVLSL"
     gene            195..707
                     /locus_tag="ACG8KQ_002986"
     CDS             195..707
                     /locus_tag="ACG8KQ_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105829.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          307 a           65 c          106 g          244 t
ORIGIN      
        1 ttattgaaaa attaaatagg tttcaagaaa tagttgaaga tagtgatttt aacactttta
       61 atagccataa accgatgaga ggtacttata taaaatttct cgaaaattat aatgatttaa
      121 aatctaccaa taattatcaa tttggaaact tgtcttatga gatgataagt aaagttctaa
      181 gtttgtgatg ataaatgaaa ttaaatagta taaatgattt atttgctatt ataggtgtta
      241 ttgctaacat acttttttcg tgtttgaatt ttagatttac tcaaaagaac aaaaagaaca
      301 aactcagtat agaacaaatg aaaaaagaca ttgaaactga tttaaaaaat gaccataaag
      361 aaagaagaaa aattgaagaa tatacttctt taatggaaaa gtgtaaagca actgatgcaa
      421 tttttagctt tttaagaaaa agtgatgaga taatcacacg tgaaaatata gatttagaaa
      481 atgagattag taaacttgaa tctatctttt ccgatataaa ttctcttaag tatttaatcg
      541 tcgaagaaaa ttcggatagc tttttagaat ttgaaaaata tctaacagat cttatagata
      601 atagaaataa tgaattaatg agacaaaaca ttgcaagatt ccttactaaa ttagtttctg
      661 aatggaaaag tattatttct caaaatatgg ataaattaag atattaagga tgttagtttt
      721 aa
//
LOCUS       DAZKCE010000259          707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000259 DAZKCE010000000
VERSION     DAZKCE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.270"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..598
                     /locus_tag="ACG8KQ_002987"
     CDS             <1..598
                     /locus_tag="ACG8KQ_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9105830.1"
                     /translation="MKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVG
                     GGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKK
                     GQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLV
                     NGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          199 a          139 c          180 g          189 t
ORIGIN      
        1 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
       61 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      121 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      181 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      241 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa aacgcttagg
      301 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      361 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      421 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      481 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      541 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      601 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      661 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKCE010000260          693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000260 DAZKCE010000000
VERSION     DAZKCE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.271"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8KQ_002988"
     CDS             132..344
                     /locus_tag="ACG8KQ_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105831.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>693
                     /locus_tag="ACG8KQ_002989"
     CDS             506..>693
                     /locus_tag="ACG8KQ_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9105832.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct aga
//
LOCUS       DAZKCE010000261          685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000261 DAZKCE010000000
VERSION     DAZKCE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.272"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..627
                     /gene="spxA"
                     /locus_tag="ACG8KQ_002990"
     CDS             229..627
                     /gene="spxA"
                     /locus_tag="ACG8KQ_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9105833.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          243 a           99 c          132 g          211 t
ORIGIN      
        1 ttagaaaatt tgaattagat ttgaaaactc taatttttaa atgataaaaa aagcgagaat
       61 gctggtttta ctgtatatta accttagata actttcattt ttttaaaaaa attatgaaaa
      121 aaaggtagct atttaaaagt tgattatgta taatagaaac agatgggaga ttattatgat
      181 cttctgttac aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta
      241 tatacttccc caagttgtac ttcctgcaga aaggctcgtg cgtggttgca agaacatcag
      301 attccatttg ttgaacgtaa tattttttca gaaccattga acagttctga attaaaagcg
      361 attttacaaa tgacagaaga cggaacagaa gaaatcatct cgacacgttc caaagtattc
      421 caaaaactga acatggattt agatgatctg ccgttacaag aattattgga acttgtgcaa
      481 aacaatcctg gattgcttcg ccgtccaatc atgattgatg ataaacgtct gcaagttggt
      541 tttaatgaag atgaaattcg tcggttccta ccgcgcgatg tacgacaatt agaattacgt
      601 caagcacaat tgatggctgg attatagaag gataatacga aacttggaaa agactggctc
      661 tttgtcaaat gatgttggta aagag
//
LOCUS       DAZKCE010000262          676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000262 DAZKCE010000000
VERSION     DAZKCE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.273"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>676
                     /locus_tag="ACG8KQ_002991"
     CDS             <1..>676
                     /locus_tag="ACG8KQ_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9105834.1"
                     /translation="EVKGLLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLL
                     EFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSE
                     ISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISN
                     IGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFR
                     KQEVNK"
BASE COUNT          294 a           72 c          120 g          190 t
ORIGIN      
        1 cagaagtgaa aggattactc aataaactaa ctatttctaa tatagactcg actagggaaa
       61 aaatcataaa aatatttgat aaaattaata atttattcaa gaatgacaat gctgaattaa
      121 ggaagggtct tttagagttt aaacttcaag aactagaact tcattactct tatttagtga
      181 aaattaccga agaaaaagaa caacaaaaag caataaaaga acagatgata gaagaggaaa
      241 aagttcgtag agaaattgat cgtgaaaaga aacgtattga taaagaacaa cgtcaattca
      301 attctgaaat ttccaaatta atcgcatata tgcaaaaagc taatgcagat gtggaaaaag
      361 aattatatgc aaataaaatt caagaactgg aagaaaaatt aaaagaatta gaagttatac
      421 gtgaaaatgt attacaaaga gaattaaata ctagagccgg ttatgtgtat gtaattagta
      481 atattgggtc ttttggtgaa gacatatata aaattggtat gactagaaga ttagaaccta
      541 tggatagaat aaaagaattg agtagtgcat cggttccatt tgaatttgac gttcatgcga
      601 tgattttttc agaggacgct ccgtctttgg aaactaaatt gcataatcat tttagaaaac
      661 aagaagttaa taaaat
//
LOCUS       DAZKCE010000263          672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000263 DAZKCE010000000
VERSION     DAZKCE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.274"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..265
                     /locus_tag="ACG8KQ_002992"
     CDS             <1..265
                     /locus_tag="ACG8KQ_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105835.1"
                     /translation="NQFIFPLLNMIALLPVEETAIIAETLLNLTSFKRKYTTSVETVG
                     GPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRLRKERANDYNN"
     gene            249..419
                     /locus_tag="ACG8KQ_002993"
     CDS             249..419
                     /locus_tag="ACG8KQ_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105836.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          253 a           91 c           99 g          229 t
ORIGIN      
        1 aaatcaattc atatttccgt tactaaacat gattgcttta ctcccagttg aagaaacagc
       61 aattatcgca gagactttat taaatttaac cagttttaaa cgaaaatata ctacttctgt
      121 agaaacagta ggtggcccaa ttgatgtttt agctattaca cctaatgatg gccctatttg
      181 gattaaaaga aaacattatt ttgatattga taataatatt ggatacagat tgcgaaagga
      241 gagagcgaat gattacaata attaaacctc aggaaaaatc ccctgttttg aaatttaatg
      301 agagcaatct agcccataat aaaatgagta taactgatac agtatttgat gtaatggttg
      361 agcaacaaaa aaaaattaat aataacaata gacaaagaaa agaaattaac caatattaat
      421 gttttttctg ttgcgttttt tgcagtcata atttaaaacc tcgctcaatc ttgggcgagg
      481 tcttttttta tttttctgaa aagttaaagt aaataatagc ataaaataat atattttaca
      541 aagaataagt acaatctagt tttttgctat taaatgtgta ataataaata tgccatcaca
      601 acatgaagaa tgaaatccat tattatctag tctagtccat tctttttgtt tgcagtattt
      661 gtgatggctt tc
//
LOCUS       DAZKCE010000264          634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000264 DAZKCE010000000
VERSION     DAZKCE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.275"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..504
                     /locus_tag="ACG8KQ_002994"
     CDS             <1..504
                     /locus_tag="ACG8KQ_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9105837.1"
                     /translation="KEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANN
                     NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIE
                     ISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFT
                     TQVILLF"
     gene            519..>634
                     /locus_tag="ACG8KQ_002995"
     CDS             519..>634
                     /locus_tag="ACG8KQ_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFP9105838.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKT"
BASE COUNT          251 a           73 c          104 g          206 t
ORIGIN      
        1 aaagaaataa aagaattaga gatctattct aattcttatc taaccaataa gtttgaatat
       61 cgttatttaa aaaatataaa aaatgaatat ttgaaaagtt tgttgatagc gaagctatac
      121 caagcaaata acaataagat ttgttgtaaa ttcgaatgtc caagtactat tattgaagtt
      181 cctgttccaa tatttgactg tattagggtg cttggaatta ttctcgataa tgcaattgaa
      241 gcatctatag agagtcaaga tagagaactg tctctagcta tttaccgaga tgataaacag
      301 atagaaattt cgatatcgaa tagttgtaga gagatactac tctctataga tactctaatg
      361 aaaaaaggaa taacaacaaa aaagggacat caagggttag gtttatctag tatagaggaa
      421 atcaataaaa agaatcccaa tatgtttgtg aactatcaaa acgatccgta tcaatttact
      481 actcaagtta ttttgttgtt ttgagggaag gaggatttat gagttatcca ataatattat
      541 gtgaagatca aattatacaa ttaaatcagt tagaacgaat tattgataat ttcattcttt
      601 ttcacgataa agtatttaag atagtattga aaac
//
LOCUS       DAZKCE010000265          619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000265 DAZKCE010000000
VERSION     DAZKCE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.276"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..437)
                     /locus_tag="ACG8KQ_002996"
     CDS             complement(<1..437)
                     /locus_tag="ACG8KQ_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105839.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          183 a          112 c           86 g          238 t
ORIGIN      
        1 ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa tactttataa
       61 gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat atatttttcc
      121 tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg agataacaga
      181 gctttgctag aacattttcc attaatttcc agacatatat ccaattcact gacggttact
      241 cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc gatcgttttt
      301 ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga ctctgataaa
      361 tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc tttgcttaaa
      421 aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc catgcattca
      481 ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc agcgattctt
      541 gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg ttttatttgg
      601 cctttctcgc gatcaaacc
//
LOCUS       DAZKCE010000266          617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000266 DAZKCE010000000
VERSION     DAZKCE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.277"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>617)
                     /locus_tag="ACG8KQ_002997"
     CDS             complement(<1..>617)
                     /locus_tag="ACG8KQ_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105840.1"
                     /translation="TPLNIIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFI
                     LLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLK
                     NHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQ
                     GGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCI"
BASE COUNT          231 a           79 c           81 g          226 t
ORIGIN      
        1 ttatgcaata aaaaagaaga acaattagcg tgattataga aataaataat gagattttag
       61 aaaaaaatgt gtatgaatac ctgattgtta ctttatttat tccactttca ctttttactg
      121 taacaaaagg acctccttgt tttagtactg tagcattttt tgaattaaca gtaaccgtta
      181 atcctttata ccggaataca ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa
      241 atgtaatttc atctttattt ggagaaaagc tactataaat aacttgacca tcttctgtaa
      301 cttgatggtt tttcaaaagc aattgatctt ttttatagcc tataggtaca taatcaaaat
      361 tctcaaattc ttcaagttta ttttccattt gttcattaga ataagttcct tgaaaattca
      421 tttctttcac catgttaact actgatgaat aatttaaaat tgaaatagct aatattaata
      481 atagtataaa atttcttctc cttgtataag aaaatttttc caactgtgga gaaataaata
      541 gagatactga aaaagatatg aacaatgttg aaaatatcat aaaacgccaa ggagcttgaa
      601 taatatttaa aggagta
//
LOCUS       DAZKCE010000267          591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000267 DAZKCE010000000
VERSION     DAZKCE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.278"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>591
                     /locus_tag="ACG8KQ_002998"
     CDS             26..>591
                     /locus_tag="ACG8KQ_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP9105841.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          210 a          101 c          117 g          163 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAZKCE010000268          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000268 DAZKCE010000000
VERSION     DAZKCE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.279"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8KQ_002999"
     CDS             complement(38..>583)
                     /locus_tag="ACG8KQ_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9105842.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZKCE010000269          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000269 DAZKCE010000000
VERSION     DAZKCE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.280"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8KQ_003000"
     CDS             <1..354
                     /locus_tag="ACG8KQ_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9105843.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKCE010000270          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000270 DAZKCE010000000
VERSION     DAZKCE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.281"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..343)
                     /locus_tag="ACG8KQ_003001"
     CDS             complement(41..343)
                     /locus_tag="ACG8KQ_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9105844.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          181 a           92 c           71 g          179 t
ORIGIN      
        1 ataaaatccg ttccgggcta ccttttttag gtagcctact ttaatcttta tacctttctg
       61 gatcaacgaa agtatacttc aaatagtcat aacgccgatg atcgctccgt gcgtctggca
      121 catcagtcac gatatcaaac aaaaaataca catctttctt cattctggtt tttgttgccg
      181 gaattttaaa gtagttctta ttagagtaat agagattgat taataagcta tcttcgattg
      241 ctaaaaagaa aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag
      301 gatcaaattt aaaccataat tgtgtttttc cttccatcaa cataatgttc acctcaaaaa
      361 gagtatacga actaatgttc tttttgtaaa gtaggaatat cattgataat atggatggaa
      421 catatatatt aaaaagtgtt ctttttgaat gctattttga atgccatttt cattcattta
      481 acgaaaatct gcgaaaataa aaaatgctca aacccttata aaa
//
LOCUS       DAZKCE010000271          519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000271 DAZKCE010000000
VERSION     DAZKCE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.282"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..158)
                     /locus_tag="ACG8KQ_003002"
     tRNA            complement(87..158)
                     /locus_tag="ACG8KQ_003002"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Gln,seq:ttg)
     gene            complement(168..240)
                     /locus_tag="ACG8KQ_003003"
     tRNA            complement(168..240)
                     /locus_tag="ACG8KQ_003003"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:His,seq:gtg)
     gene            complement(302..386)
                     /locus_tag="ACG8KQ_003004"
     tRNA            complement(302..386)
                     /locus_tag="ACG8KQ_003004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Leu,seq:caa)
     gene            complement(407..477)
                     /locus_tag="ACG8KQ_003005"
     tRNA            complement(407..477)
                     /locus_tag="ACG8KQ_003005"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Cys,seq:gca)
BASE COUNT          152 a          133 c          114 g          120 t
ORIGIN      
        1 cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg ccaattccgc
       61 cacgccggca aatttaaaaa taataactgg ggtagctgga ttcgaaccaa cgcatgaggg
      121 agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaggc gaatgatggg
      181 aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc
      241 catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca
      301 ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc
      361 gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga
      421 tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt
      481 atactattat caatctataa aataaagaat aaattatag
//
LOCUS       DAZKCE010000272          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000272 DAZKCE010000000
VERSION     DAZKCE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.283"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          175 a           66 c           70 g          206 t
ORIGIN      
        1 tagatataat acaaaaaggc aaagatattt tcaaaacatt ttttcttgat gtgatatcct
       61 tgtctttttt gtaatgtttt taacttattg cttttgcaaa atttatttac ctgttttctg
      121 gatcaacgat agtgtatatt atgctatcct tgtttggatt ttaggacgtt taaagaggca
      181 cttttttaca taacttaatc catagacctt ttaagcatgc tgtgagataa taataaagaa
      241 gagtttaaag cgcaccccaa accacttccc cataagtgta ttacgcttta aactctttta
      301 tatttgaatt cactaagaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttat
      361 aattaggatt tagagtaatt aattagatgt ttattttata ggactgatat tatagattac
      421 aaatagtatc gtatttcgca atcttaaaat tatcttctta ctatgaataa gataataaaa
      481 tttgtatttg tcctttttag gtaacttatt ttaatct
//
LOCUS       DAZKCE010000273          515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000273 DAZKCE010000000
VERSION     DAZKCE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.284"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACG8KQ_003006"
     CDS             <1..231
                     /locus_tag="ACG8KQ_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFP9105845.1"
                     /translation="SRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITL
                     DKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            343..>515
                     /locus_tag="ACG8KQ_003007"
     CDS             343..>515
                     /locus_tag="ACG8KQ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9105846.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          214 a           70 c           75 g          156 t
ORIGIN      
        1 agtcgtgtaa aattaagaat tagctttgaa aaaaatacaa agagaatagt aggtgtacaa
       61 ctttgtagtc aagaagattt atctggacta ttatcaatgt tttctcttgc tatagaagaa
      121 caaattacat tagacaaatt gaaaatgtta gattatttat ttcatcctag ttttagccaa
      181 ccctataatt ttattttaaa ggcagtaaca ggattaaaca ttgaaacata atcgttacta
      241 aatgaaatca agaaatatac tgtattactc aattcattga aaatatttat cataattttt
      301 aatagaaaac actattaaag agaaaaaaga gaggtaaatc aaatgaaaaa atctttaaaa
      361 cttgcatcag cagttatatt aatgtcccaa ctccttatag caacaccttt ccatgtgttg
      421 gcaagcgaaa attcaacata caaaattgca aaagaaagaa ccgagacgac tgaaatgagt
      481 aattccgatg taacagcaga gagtagtgaa ctttt
//
LOCUS       DAZKCE010000274          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000274 DAZKCE010000000
VERSION     DAZKCE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.285"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8KQ_003008"
     CDS             complement(<1..132)
                     /locus_tag="ACG8KQ_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9105847.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKCE010000275          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000275 DAZKCE010000000
VERSION     DAZKCE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.286"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG8KQ_003009"
     CDS             <1..343
                     /locus_tag="ACG8KQ_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105848.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAZKCE010000276          509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000276 DAZKCE010000000
VERSION     DAZKCE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.287"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          170 a           96 c           58 g          185 t
ORIGIN      
        1 tttttaaaaa taagaatgct aaatcactct acaataacgg cgaaagcaca aaaaataaaa
       61 aaattattac gttattaaaa aaacagatga aagatcaggt tttattagat ctcattcttt
      121 catcacttga agggatttaa tgaactactt tttcctcttt gtcgttttta tatattcgta
      181 ttccttcaaa cagtaggata gcctctttca atgcttgata tttgaactgt ccgttactta
      241 aaaatttaga tgtgccgccc tcttcctaga acatattctt ttttactttt tcattaattg
      301 acagaccaat cgaacgaaac tgtaaatttc gataggataa tgattggtca aactcattca
      361 ttttcaagac atctcgattc atattcaaga taggaatctt tttatcaaca tcactttatt
      421 cgtattttcc tctaccataa aagcttcacg ctccacaaaa ttgttttata cactactatt
      481 cagcccattc aacgaccaat gaatttttt
//
LOCUS       DAZKCE010000277          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000277 DAZKCE010000000
VERSION     DAZKCE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.288"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG8KQ_003010"
     CDS             43..165
                     /locus_tag="ACG8KQ_003010"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105849.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACG8KQ_003011"
     CDS             138..269
                     /locus_tag="ACG8KQ_003011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105850.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAZKCE010000278          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000278 DAZKCE010000000
VERSION     DAZKCE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.289"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>482
                     /locus_tag="ACG8KQ_003012"
     CDS             <1..>482
                     /locus_tag="ACG8KQ_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105851.1"
                     /translation="FMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGM
                     DYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPV
                     YAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYL
                     "
BASE COUNT          179 a           68 c           84 g          151 t
ORIGIN      
        1 tttatgactg ttttgttatt aatcacttta actgtttcta gtaaaaaaaa tttcaatcaa
       61 aaaataattt ctcagccggg acatattgtt gtaaacaaag aaacagtaga ggcgtatacc
      121 actaaaggaa tggattattt tttatatgat tattctccta tcgcttcatt ggagaataag
      181 gagcatataa taaaacacga agcaaagatt ggcagtcaat gggtaaacct tccttataaa
      241 acaactggta gttctatcat atatagagta actaatgaga agagccaagt aatcacactt
      301 cctgtatatg cttatttagg aacgaatgta gaaataaatc atcaacctgt aaaggagcag
      361 gtgaatgcta atggattggt acaaataagc ggagtcaaag gagttgctga tattaaagta
      421 agcacaagtt atacagtaac gactattatt tcttatcttg ttacattaac tgcatattta
      481 tg
//
LOCUS       DAZKCE010000279          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000279 DAZKCE010000000
VERSION     DAZKCE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.290"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8KQ_003013"
     tRNA            complement(20..91)
                     /locus_tag="ACG8KQ_003013"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8KQ_003014"
     tRNA            complement(113..200)
                     /locus_tag="ACG8KQ_003014"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8KQ_003015"
     tRNA            complement(225..298)
                     /locus_tag="ACG8KQ_003015"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8KQ_003016"
     tRNA            complement(312..382)
                     /locus_tag="ACG8KQ_003016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8KQ_003017"
     tRNA            complement(384..459)
                     /locus_tag="ACG8KQ_003017"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKCE010000280          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000280 DAZKCE010000000
VERSION     DAZKCE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.291"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>455
                     /locus_tag="ACG8KQ_003018"
     CDS             <1..>455
                     /locus_tag="ACG8KQ_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFP9105852.1"
                     /translation="NRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRKKRD
                     TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIE
                     QDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          154 a           91 c           91 g          119 t
ORIGIN      
        1 aaaatagaca atccttctat tcatggaaaa tggacgaaac ctatatcaaa attaagggac
       61 gttggcatta tctttatcgt gcaattgatg cggacggctt aaccttagat atctggttac
      121 gaaagaaacg ggatacgcaa gcagcctatg ctttcttaaa acgactccat aaacagtttg
      181 gtgagccgaa agcaattgtg accgataaag caccttctct tggctccgcc tttagaaagt
      241 tacagagtgt gggtttatat actaagacag agcaccgaac tgtgaagtat cttaacaatt
      301 taatagaaca agaccatcga cctattaaac gacgaaataa attttatcaa agtctccgta
      361 cagcctcttc cacgattaag ggcatggaga ccattcgagg aatatataaa aagaaccgaa
      421 gaaatggaac gctcttcggc ttttcggtgt ctact
//
LOCUS       DAZKCE010000281          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000281 DAZKCE010000000
VERSION     DAZKCE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.292"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG8KQ_003019"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG8KQ_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAZKCE010000282          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000282 DAZKCE010000000
VERSION     DAZKCE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.293"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8KQ_003020"
     rRNA            <1..>411
                     /locus_tag="ACG8KQ_003020"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKCE010000283          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000283 DAZKCE010000000
VERSION     DAZKCE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.294"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8KQ_003021"
     CDS             <1..>386
                     /locus_tag="ACG8KQ_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9105853.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKCE010000284          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000284 DAZKCE010000000
VERSION     DAZKCE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.295"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8KQ_003022"
     CDS             <1..>386
                     /locus_tag="ACG8KQ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9105854.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKCE010000285          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000285 DAZKCE010000000
VERSION     DAZKCE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.297"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>369
                     /locus_tag="ACG8KQ_003023"
     CDS             98..>369
                     /locus_tag="ACG8KQ_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105855.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DAZKCE010000286          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000286 DAZKCE010000000
VERSION     DAZKCE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.298"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8KQ_003024"
     CDS             complement(<1..272)
                     /locus_tag="ACG8KQ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105856.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZKCE010000287          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000287 DAZKCE010000000
VERSION     DAZKCE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.299"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8KQ_003025"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8KQ_003025"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKCE010000288          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000288 DAZKCE010000000
VERSION     DAZKCE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.300"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8KQ_003026"
     CDS             <1..>344
                     /locus_tag="ACG8KQ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFP9105857.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKCE010000289          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000289 DAZKCE010000000
VERSION     DAZKCE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.301"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8KQ_003027"
     CDS             <1..>341
                     /locus_tag="ACG8KQ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9105858.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKCE010000290          326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000290 DAZKCE010000000
VERSION     DAZKCE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.302"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..206)
                     /locus_tag="ACG8KQ_003028"
     CDS             complement(<1..206)
                     /locus_tag="ACG8KQ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105859.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT          130 a           61 c           28 g          107 t
ORIGIN      
        1 cctatctttt ttacaaatat ttcatttggt gtacagacta ataaaacaat atttgtaatc
       61 aatccttgca ctaaagaatt aaaaggaaga ctatttaaaa cagggtgtgc ataactataa
      121 catgtgaagt agttattttg aactttaact tttaaaaact ttaaaaataa tcttatttgt
      181 tcttctaaac aatcatctat actcatataa tcactccact aattaatctc cccaaaaaga
      241 cacagcatta agagatatac caacacaaca gtgcaaataa ctctacatct tccttcgcaa
      301 ccttatttca ataaaccata aaaatt
//
LOCUS       DAZKCE010000291          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000291 DAZKCE010000000
VERSION     DAZKCE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.304"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACG8KQ_003029"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACG8KQ_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZKCE010000292          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000292 DAZKCE010000000
VERSION     DAZKCE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.305"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG8KQ_003030"
     CDS             <1..>299
                     /locus_tag="ACG8KQ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9105860.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZKCE010000293          281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000293 DAZKCE010000000
VERSION     DAZKCE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.denovo.307"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          104 a           44 c           37 g           96 t
ORIGIN      
        1 ttctcacttt ttttcaaaaa tatggtatgc tttttaatgg cttcaaatat aaaagagttt
       61 aaagcgtaac acacttatgg ggcagtggtt tttggggaac gctttaaact cttctttatt
      121 attatctcac aatataaccc aaatgtcttt ctatttaaaa atcaaagtaa aacttttcaa
      181 atatacagaa atataactat gtgaaacatc ctttcattaa tccataaaag gatacataaa
      241 aaagccactc atttgagtgg caatgaagaa aagctttagc t
//
LOCUS       DAZKCE010000294        14934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.069, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000294 DAZKCE010000000
VERSION     DAZKCE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.069"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(382..3198)
                     /gene="uvrA"
                     /locus_tag="ACG8KQ_003031"
     CDS             complement(382..3198)
                     /gene="uvrA"
                     /locus_tag="ACG8KQ_003031"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9105861.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(3208..5202)
                     /gene="uvrB"
                     /locus_tag="ACG8KQ_003032"
     CDS             complement(3208..5202)
                     /gene="uvrB"
                     /locus_tag="ACG8KQ_003032"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9105862.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5461..6963
                     /locus_tag="ACG8KQ_003033"
     CDS             5461..6963
                     /locus_tag="ACG8KQ_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9105863.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            6965..7702
                     /locus_tag="ACG8KQ_003034"
     CDS             6965..7702
                     /locus_tag="ACG8KQ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9105864.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7873..9066)
                     /locus_tag="ACG8KQ_003035"
     CDS             complement(7873..9066)
                     /locus_tag="ACG8KQ_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9105865.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(9145..9351)
                     /locus_tag="ACG8KQ_003036"
     CDS             complement(9145..9351)
                     /locus_tag="ACG8KQ_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9105866.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(9344..10486)
                     /locus_tag="ACG8KQ_003037"
     CDS             complement(9344..10486)
                     /locus_tag="ACG8KQ_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9105867.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            10998..11633
                     /locus_tag="ACG8KQ_003038"
     CDS             10998..11633
                     /locus_tag="ACG8KQ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105868.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            11787..11864
                     /locus_tag="ACG8KQ_003039"
                     /pseudo
     CDS             11787..11864
                     /locus_tag="ACG8KQ_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            12178..12225
                     /locus_tag="ACG8KQ_003040"
                     /pseudo
     CDS             12178..12225
                     /locus_tag="ACG8KQ_003040"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12241..14160
                     /gene="tet(M)"
                     /locus_tag="ACG8KQ_003041"
     CDS             12241..14160
                     /gene="tet(M)"
                     /locus_tag="ACG8KQ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9105869.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14279..14446
                     /locus_tag="ACG8KQ_003042"
     CDS             14279..14446
                     /locus_tag="ACG8KQ_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9105870.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14506..14859)
                     /locus_tag="ACG8KQ_003043"
     CDS             complement(14506..14859)
                     /locus_tag="ACG8KQ_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9105871.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         4384 a         2966 c         2797 g         4787 t
ORIGIN      
        1 gcatatgctt ggcagatttt gccgtatgct tcattaaaag aacgtgccat accataaacg
       61 ttcatgtcgc ttcctaataa tatagggata aattcttcat tttcaatact gttcatcatt
      121 atctctcact tactcataat ctcttgtttt tcttttccaa ctacacaagg aatatcatac
      181 cataaagaaa aaagcaatca aaggaaagtc ctccgattgc tcaattcttt tttgaacgga
      241 ttgaacttat aggaagtttt tatctatacc aatcagtgta gaaagaacta ccatgtaatg
      301 attctagaac actgatatta ataaaacaag caaaaattca tttttttatg tttttttaac
      361 tatctcagct atctatcctc tttataccat tatttttttc aaatagtgac ctgtataact
      421 ttcttttacg ttaatgattt cttctggtgt tccagtcgca aggatcgttc ctccaccttc
      481 tccaccttct ggtcctaaat caatgatata atctgctgtt ttgattacat ctagattgtg
      541 ttcaatcact aaaactgtgt taccagcatc tactaatctt tgtaatacat gcaacaagcg
      601 ggcgatgtca tcgctatgaa gtcctgtcgt tggttcatct agtatataga aattctttcc
      661 attagagatt ttgtgcaatt cactggcaag tttcatccgt tgtgcctcac caccggacaa
      721 tgtcgttgct ggttgcccca tcgtcacata acctaagcca acatcaacaa tcgtttgcag
      781 tttgcgatga atctttggaa tgtgcttgaa gaattctaca gcatcctcta ctgtcatttc
      841 caaaatatca gcaatgcttt ttcctttgta atgcacttct aatgtttcag aattatatcg
      901 tttaccatga catacttcac aaggaacata gacatcaggc aaaaagtgca tttcgatctt
      961 aataattcca tccccgcgac aagcttcaca acgaccgcct tttacgttga aactaaaacg
     1021 ccctttctta taaccccgca tttttgcctc gttcgtttga gcaaataacc cacggatatc
     1081 atcaaataca cttgtataag tcgctggatt acttctcggc gtccggccga ttgggctttg
     1141 atcgatatcg atgatctttt cgatactttc gtagccggaa attgtcttga atttacctgg
     1201 cttagcagaa ttcttattta atttttgcgc taacgatttc tttaagatac tattgaccaa
     1261 tgtactcttt ccagaaccag acacacctgt gactgctaca aattctccta atggaaattc
     1321 aacactaata tttttcagat tgttttctga agcaccagtg atcttgattg ctttcccatt
     1381 acctttacgg cgttcttttg gtacagggat tgattttttc cctgaaagat atttccctgt
     1441 caaagaattt tcatcattag ctacttcttc aggagtgcct gcagccacga tttgtccgcc
     1501 ttgttctccg gccccaggtc cgacgtcaat caaataatct gctgcccaca tcgtatcttc
     1561 atcatgttcg actacaacga gggtgttgcc aagatcacgc attttcttta atgagccaat
     1621 cagtcgatcg ttgtcgcgtt gatgcaatcc gatcgatggt tcatctaaaa tatacaaaac
     1681 tccagaaaga ttcgatccaa tttgtgtagc caatctgatt cgctgcgctt ctcccccaga
     1741 taatgttccg gaagcgcgac tcaaagttag atagtctagt cccacatttt ccaagaatga
     1801 aagtctgtca tttacttctt ttaaaatcgg tttggcaatt actttctctt gttcagataa
     1861 cgttaagttt gagaagaaat ctactgcttt ttgaatagac aactcatttg tttgcccgat
     1921 attcgttcca ttgatttgaa cagatagagc ttggggattc aatcgataac ctttacatgt
     1981 ctgacaagtt aattctgtca tataaagacg catttgctca cgcgtgaaat cactgttcgt
     2041 atcatgataa cgacgctcga tattcttcaa taccccttcg aaagtcgttt ctacatcgcg
     2101 aacgccacca aaatcatttt cataatggaa atggaaaaga tcatctgtcc cattcaagat
     2161 gatctcctga tgctcttccg gtaattcttc gaatggtgta tctaaatcga taccgaaact
     2221 ttcacaagcc tgcgctaaca tttgaggata atactgagag ctgattggat tccatggaac
     2281 gatagcacct tgactaagtg tcttcgttct atctggaatc actaaatctt gatcgacttc
     2341 taatttgaca cctaaaccat cacagtctgg acaagctcca aaaggcgcat tgaatgagaa
     2401 cagtcttggt tctagttcgc caacagtaaa tccgcaataa ggacaggcat agtgttcgct
     2461 aaacaacatt tcttcttcac cgatcacgtc aaccagagca tagccgtctg ctaatcgcag
     2521 ggccgcctct aaagaatcaa ataggcgaga acgaatccct tctttcacaa tgatccggtc
     2581 gatgacgatc gcgatatcat gttttttgtt tttctccagc tcaggcactt cacttaaatc
     2641 atagatctca ccatcgactc gaacgcggac gtatccctct ttttggattc gttcgaatat
     2701 ttttttatgt tgtccttttt tctttaccac gatcggtgcc aacacttgga tctttgtgcg
     2761 ctcaggcaaa gccattactt gatctgccat ttgatctact gattgactgg taatctcgat
     2821 atgatcgttg ggacaaatcg gatgtcctac acgtgcaaac aacaagcgta aatagtcatt
     2881 gatttccgtc acagtaccta ctgtcgaccg aggattttta cttgttgttt tctgatcaat
     2941 agaaatcgcc ggactcaatc cgtcgatgct atctacgtcc ggcttatcca tttgtcctaa
     3001 aaactgacga gcataagcag aaaggctctc tacgtatctc cgctgccctt cagcgtacag
     3061 cgtatcaaat gctagggaac tttttccaga accggacaaa cctgtaacga ccacgaattt
     3121 atctcgtgga atcgttacat ctatattttt caagttatgg gcacgtgcac catgaatcgt
     3181 aattttatcg ttagccatgt tgtcctccta ctttgaagct tttaattcta aaatcatatc
     3241 tcgcacattc gcggctgttt caaaatccaa agcttttgcc gcatctttca tttctttttc
     3301 taatttcatc aataacgtgt ctttttcttc cttcgtcatt tcttcgtacg agacttgtac
     3361 cgcttctttt gtatcatctt ccgattcttt cgtaatagaa atcaaatctc ggatttcttt
     3421 aatgatcgtt tttggaacga tcccatgttc ttcattgtac ttctgctgaa tcgtacgacg
     3481 tcttgacgtt tcatccatcg ctagacgcat agaatctgtt actttgtctg cgtacatgat
     3541 tactttacct tcttcattcc gagcagctcg tccaatcgtt tggactaagg aacgttcact
     3601 acgtaaaaag ccttctttat ccgcatccaa gatagcaaca agggaaactt caggtacgtc
     3661 taatccttca cgaagcaaat tgattccaac taacacatca aattcaccta atcgaagatc
     3721 tcgaatgatt tctgttcgtt ccagcgtttt gatatcacta tgcaaatatt tgactttgat
     3781 ccctaattct ttgaaataat ctgtgaggtc ttctgccatt tttttcgtca atgtcgttac
     3841 aaacacccgc tggtcttttt cgactcgttc atttatttcg cctactagat catcgatttg
     3901 tcccatgatt ggacgaactt cgatcactgg gtcaagcagc cctgtcggac ggatgatttg
     3961 ttggataacg gtatctgttt gttcatgttc atatggtcct ggcgtcgcag agacataaat
     4021 gatctgattg acatgttttt caaactcttc taaccgaagt ggccgattgt ctaaagcaga
     4081 tggcaaacgg aaaccgtaat ctactagcat ctgttttctc gctcggtcac cattatacat
     4141 tcctctgatt tgaggcatgg ttacgtgcga ttcgtccgcc acgatcaaaa aatcttcagg
     4201 aaagaaatcc aatagcgtat aaggcggttc accttcttta cgtccgtcca tatgccgaga
     4261 ataattttca attcccgacg tgtagcccat ttctaacatc atttcaatat cgtaattggt
     4321 tcgctgttct aaacgttgag cttccagaag tttgttttca tttcgcaaga cagttaaacg
     4381 ctgttctaat tcctctttga tattcgctac ggcatgctcc atatgttcat cattcgtcac
     4441 aaagtgagtc gccgggaaga ttgctacgtg ttccgtttct cctaaaactt cacctgtcag
     4501 tgcattgact tctcggatac gttcgacctc atctccaaaa aactccacac gtaaggcacg
     4561 ttcatctctt gaagccggaa aaatctccac gacatcaccg cgcactcgaa aacgaccgcg
     4621 ctgaaaatct atatcatttc tttcgaattg gatactgact aagtcacgaa tcaactgatt
     4681 gcgatctagt tctgctccct gtcgaatcga aactacttgt ttttggtact caaaaggcga
     4741 gcctaatcca aaaatacatg aaacggatgc aatgacgatc acatcatttc tttctaataa
     4801 tgaactagta gccgaatgtc gtaatttgtc gatctcatca tttacacttg aatctttttc
     4861 gatatacgta tcgcttgaag gcacataagc ttcaggctga taataatcat aataagaaac
     4921 aaaatattca acagcattgt tagggaaaaa ttccttgaat tccccgtaca gctgaccagc
     4981 caatgttttg ttatgggcaa taatcagtgt tggtttattc actttctcaa taagattgga
     5041 aatcgtatat gttttccctg ttccagtcgc acctaataag atttgtgctt ttttcccgcc
     5101 gactacgcca tccactaatt ggttaatcgc ttcaggctga tcaccagccg gttggtattt
     5161 cgagacaagc tcaaagtgtc gggaggtatc tctctcaatc attcttcgtc ctccttttca
     5221 ttcattcctt ttcattttac gaccaataaa aagaaaagga aagcaaaggc attccttact
     5281 aaaaaattat accacaccga acatactttc gccacttttt tatcttatta tacatgttat
     5341 gttttataac ataaaatgtt agaaactcgc atttttttca ttttttcgag cttacagtct
     5401 tgtctgattc agcacttttc gcctataata ataaaatatt ggaaatttag gaggttgatt
     5461 atgaaaagaa agcatttatt tcactcattt gttttcttaa tgacaggact tgtgacactt
     5521 gcgtttggtc atgttgcaca agctgacaac actacaacgg cagagtcaag cacaagcgag
     5581 acaatgcaaa aaatagagcc caaaaaagac gtatacgtaa ttgccagtga ctctgctttt
     5641 gctccatttg aatttcaaaa cgcaaagggc gagtacgaag gcatcgatgt aaaattgatg
     5701 gaaaacatcg ccaaactgca aggatttaac atccgaatgg actaccgtgg tttctctgct
     5761 gctttgcaag ctttggaatc aggacaagca gacggaatga ttgccggaat gactgtaaca
     5821 gatgaacgaa agaaaagcta tgatttttct gattcttact ttgatagcgg gatccaatta
     5881 gctgtcaaaa aaggagatac ttcgatcaag tcttatgaag atttgaaagg aaaaacagtc
     5941 ggagcaaaag ttggtaccga aagtgctgaa tttttaagcg accatgaatc tgaatatggc
     6001 tattctatca aatattttga tgctgctgat caattgtacg acgggttaaa agttggcgca
     6061 atcgatgcaa tgatggatga ttatccggta atcggttatg cgatcaaaca aggacaagaa
     6121 ttggaaacac cgatcaaaaa agaatctggc ggaaaatatg catttgccgt taaaaaagga
     6181 caaaatcctg agcttcttaa tatgttcaat gaaggattga aagaattaaa aagaacaggc
     6241 gaatacgata agattattaa agaatatgta gcggatggtt cagaaacttc cagtgagtct
     6301 tccactgctg atgaatcaac atttcttgga cttatccaaa acaactatca agcattgttg
     6361 aaaggacttt ggagaacaat tctcctaact ttgatctcct ttgctttggc tatagtaatc
     6421 ggtgtgattt ttggattatt cagtgtagca ccaatacgcg ctcttcgcac aattgctagt
     6481 atttatgtcg atatcatccg aggaattcca atgatggttc ttgcgttctt tattttcttt
     6541 gggttgcctg gtatcgttgg atttacgatt cctgacttcc ttgctggtgt tatcaccttg
     6601 actcttaacg caagtgctta tatcgcagag attgttcgag gcgggatcaa cgccgttcct
     6661 gtcggacaaa tggaagcttc cagaagttta ggtctttctt ataatcggac aatgcaaaaa
     6721 atcattttgc cgcaagcgat acgcatcatg atcccatcat tcgtcaatca gttcgttatc
     6781 tcattaaaag acacaacgat catttccgca atcggtgtag tcgaattgct gcaaactggt
     6841 aagatcattg ttgcacgtac aacacaaagt acgtatgttt acttgatcat tgcgatcatg
     6901 tacttgattt tgatcacagc attgacaaaa ttagcaaaag ttttagaaaa gaaggtgaag
     6961 taagatggca gaaaaaatct tagttgagca tttagtcaaa aaatacggcg ataacacagt
     7021 attaaacgac atcaatgtat ccatcaacga aggagatgtc gtctgtatca ttggcccttc
     7081 aggttcagga aaaagtacat tcttgcgttg cttgaatcgt ttagaagaac cttctgacgg
     7141 tgaaattatt attgacggcg cccacttgat ggataaaagc acagatatca acttggtgag
     7201 acaacatatc ggaatggtat tccaacactt taacctattc cctcatcttt ctattttgga
     7261 aaacatcatt ttagcaccga tggacctgaa gaaagaatct agagaagaag cagagaaaaa
     7321 agcaatcaaa ttactgcaga cagttggtct agaagataaa aaagatatgt atcctgaaat
     7381 gctttctggt ggacaaaaac agcgtgtggc tattgctcgt gctctagcga tgaaccctga
     7441 catcatgctt tttgatgagc ctacttctgc actcgatcct gaaatggtcg gtgatgtctt
     7501 gaacgtcatg aaaaagttag cagaacaagg tatgacgatg gttatcgtta cgcacgaaat
     7561 ggggttcgct aaagaagtgg ccaatcgcgt catgttcatt gacgacggaa actttttaga
     7621 agatggaacg cctcaacaaa tctttgaaaa tccgcaaaat gaacggacaa aagatttctt
     7681 gaataaagta ttaaatattt aaaataaatt atatatgtct ttttgactat ctttttatat
     7741 cttcttaaat gtccgcaaag ccttatttta taggctctac gggcattttg cttttgtggt
     7801 aaacctcaca tatctaggtc tatcttctta tattttcact atcaagcgtg gttaaaatcg
     7861 tggtaaatac ttctatgcaa ttagacgctg aacctctgat tttgcggtat ctatggacgc
     7921 atgagcatac cagttcattg tgatactgat atttgaatgc cccatgatat actgtaaatc
     7981 ttttgggttc atgttcttgc ttgccagtct tgtgcagaat gtatggcgta gcgtatgcgg
     8041 tgtgatatgt ggcaatggat tgtccttgtg gtgcttgttg tatttcttta ccatacggat
     8101 aaataaggcg ttataatcaa tcgcaacttt gggcttgccc ttatgattga caaataggaa
     8161 gttgccccgt ccgtctatca caaatggttc tgcctttgga cgttttttca taacctgttg
     8221 aaatgcctgt atcgtttctt tgcttaatgg tacttgcctt attccgcttt ttgttttagg
     8281 cgtttcaata taatagccct gttccttgct ctttagtaac tggtggtcga taatcacaac
     8341 ttcattcttg aaatcaatgt cggctactgt cagtccgcac agttccgaaa tacgaagtcc
     8401 tgtctttaac agtatcagca catcatcata atgcttgtga tacacattgt ccgtcttgat
     8461 gaatgagagt aaggcttgtt cctgttcctc tgtcaatgcg actttctttt tggtatcatt
     8521 ttctaggact tcactcaact tgaaatcaaa gggatttttc ctcacataat catcttgaat
     8581 ggctatgtag aatgacgctt ttaacgagcg tttatggttg ttaatggtat tgtaggaaaa
     8641 gcctttgtct ttcatgcgta acgcccattc cttagcgtca gagggtttga ttgtatcaat
     8701 gctcctagca cctaacttgt cctctttcaa taaccgcatg agttgttccc gttgtttctg
     8761 tgtgcttttc ttcacatttg ccctctgtgc gttctgtttg gcataaagtt ggcaaagcgt
     8821 cattttcttg ccagtgctgt cgataccgtc ctcaatatcc cgtcttatct gctgttccag
     8881 ttctcgaagt gagggttttt cccgttttcc ctttggtgtc gggtctgtgg gtgtcaatct
     8941 ccaagcatac acatattttg tgtttccaaa tgcgtctaca tatttatata agtattttct
     9001 gtctgttcgt tggctctctc cagtatgcag gatacgacct ttgttatccc gtcttttttc
     9061 tttcatggtg tctgctcctt tccttgttgg aaagagcctt gatatgactt gtgttcatca
     9121 taacacatac aaggctcatt tgcattagat agcgtccaat ttatcaatga cctgttcaaa
     9181 ctgtcggcgt ttaatctgta tgcggttgcc attcataatg agccagccag cgtccttgtt
     9241 ttcctctgcc aatcgtctta acttgttttc tccgatacgg aaatactttg acgcttcttc
     9301 aatggtaagg gtgtattttt cccatacggg aatatcctgt gcattactca taaaacaccc
     9361 cctcactttc gattacgtct gctatgtccg tacacatctg ttccacaagc atttcctgct
     9421 tttcagagag gtgggtacat tcatcaatca ttttcatgta gtttgagcct gtcagcctgt
     9481 cgatttcctc tatcatcacg attgacggca tgacctgtct ttccagccat tgcagagtgc
     9541 gttccatatt gacaggtacg gggttcattt caaaaggtaa actgtcatgg gaaatgaata
     9601 tctcccacag cggatagtcg ggaaattgca caaaatcagt aataaggtaa aacaccagcc
     9661 catgagggtt atatgtcaat aacaattcct caactgcctg tactgctttt gtaccacgca
     9721 gacgaatttc aaaacgattg ataatatccg tatgtttctt tttggttgcc tgttcctttt
     9781 gtttttcata caggcagaag tattttgtgc ttgcctttga accgatataa agggtgcttg
     9841 ctaaattacg gttttttccg cttagttttc caccctgcac attttcataa tttttacagc
     9901 gacaatccgc accgccattt ctgtattttt cagacaaaac gggaatatcc aacagaccac
     9961 acatatcatt gattgccaag tcaaaacgtc ggataacacc gtcacagtca agacagcggt
    10021 ttaaaaatga ataccaatct ctgttctgtg cctgcaagac atattccatg ttgcgtgagc
    10081 ctgccccttt taattccaag aatacgccca tgtgttcgtc atcagaagcc atgaccttga
    10141 tttctccgta ggcgtaatgc tctttatagc cataataccc atagtcataa tggataaaat
    10201 aatctgcttt catgccaagc acctttcgga taatttccaa tgcgtcagtc gttggataac
    10261 ggacagacag ataatcaacg agcagataaa aaggcttatc gcagatataa taattcagac
    10321 aacgcaggat aatattttgt gtttccttgc tggcgttggc ttttccgttc tcaaagcggt
    10381 tgatactata tcttgataca tgggtaagac ttgcaagttc tccttgtgaa agaccgcttg
    10441 caagacgttt gttcttcatt tcaagtgcaa attctaaatt gttcattgta ttcctccaat
    10501 cccacaatag ccttgaaata cctgtaaacc tctattcaaa aagtgtgata tgcactatct
    10561 tcttaaaact tctcattact actcacaact ttgggattgt atggtttttg acttgtcttt
    10621 tgtgaaatag tgcccccctg ttagataacg ggggcacaga caaggctcgc aggctcgcca
    10681 gccgacaaag catttcttcc ccttgacaca aggtgtcatg gcggtaagcc tgagaggctt
    10741 ccgccaccgt gtcagaaata ctttgtcagc aagttctgtt ttgccttttc taacaagttc
    10801 catttcaacc gatattcata tctacggatt taatcagata ggtatttctt tttgaatgtt
    10861 tttatatttc attctattgt gcttattcga ttacaagaac atagtaattc ttatatgctt
    10921 atttgtcaat atcttttttt gcctttacaa atcatattaa gcatgataga cttataatag
    10981 cgaagatata acgagatatg gagggttatg caatgaaaat aagccattta ggaaataata
    11041 tacagaccat aagaaaattt aggggaatga aacaacagga acttgcggac aaaatcggta
    11101 tcaatatgca gagcctttcc aagattgaaa gagggttgaa ctatcctgcc tatgaaacgc
    11161 tggaaaagat aatggaagtg ctggacgtta cgcccaatga attgttgtcg ggagaatgga
    11221 agtatgtcaa tcaatctgaa aaggaagtct gtcagttttt aagagccgaa gaacgtctaa
    11281 atgcagaact gaaacatgga cactatgata acttctttga cagcgaggaa gaatggctgg
    11341 aatatgagtt agaaaagtta cgggaatata ttacagacta tatcaacggg aaaagcattg
    11401 aagcgtccga cctttaccca atcaaagaat ttatccagca tttgaagttt caaaaattat
    11461 tagaccgcta tgatgattta tacagtatgg atatgtttgg ggaaagcata gagggacata
    11521 agtataagac accttatcaa gtcgtgaaaa tgataaaccc gaactcaaaa gaagatatgg
    11581 aactgttacg agaagtatta aggaataaca attttgatga tgaggacgag taactttttt
    11641 cctcttgcga gaagatacag caaacaaaaa gcccgatcaa gagtgcaaag caccaccaat
    11701 ggtggcaaag ctcttgacag ggctttttct tttctgttgt gggcaatcaa gaggaagaaa
    11761 gggataaaat ctgatttgtg gtaaatcagg caaaggcaac gcaggtatct caatttgatt
    11821 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
    11881 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa
    11941 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
    12001 aaggtattct taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt
    12061 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
    12121 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
    12181 aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac
    12241 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
    12301 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
    12361 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
    12421 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
    12481 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
    12541 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
    12601 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
    12661 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
    12721 tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
    12781 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
    12841 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt
    12901 gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa attttattca
    12961 tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa
    13021 aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg
    13081 gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt
    13141 gaattatgta agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt
    13201 ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa
    13261 aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg
    13321 ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat
    13381 tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt
    13441 gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat
    13501 atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct
    13561 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
    13621 gagagctcgg tttctcttgg atacttaaat caatcgtttc aaaatgcagt tatggagggg
    13681 atacgctatg gctgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
    13741 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
    13801 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
    13861 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa
    13921 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
    13981 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
    14041 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
    14101 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
    14161 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
    14221 taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca agtggatatt
    14281 gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg aattaaaaaa
    14341 attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa agcagttcaa
    14401 agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg agattaatac
    14461 aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt cttcgatgtt
    14521 tcttgcttcg ttgataccgc tggctaaaga ttccattaag gatagttctt tgtctgtaaa
    14581 gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt tattagcagg
    14641 taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa gaacttgtat
    14701 gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa
    14761 tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa tggctaaacc
    14821 aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc tctatacatt
    14881 ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg ttta
//
LOCUS       DAZKCE010000295         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000295 DAZKCE010000000
VERSION     DAZKCE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.121"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8KQ_003044"
     CDS             128..1564
                     /locus_tag="ACG8KQ_003044"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9105872.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8KQ_003045"
     CDS             1753..4200
                     /locus_tag="ACG8KQ_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9105873.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8KQ_003046"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8KQ_003046"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9105874.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8KQ_003047"
     CDS             5487..5693
                     /locus_tag="ACG8KQ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105875.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8KQ_003048"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8KQ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9105876.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8KQ_003049"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8KQ_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9105877.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAZKCE010000296         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.153, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000296 DAZKCE010000000
VERSION     DAZKCE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.153"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8KQ_003050"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8KQ_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9105878.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8KQ_003051"
     CDS             763..1917
                     /locus_tag="ACG8KQ_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9105879.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8KQ_003052"
     CDS             2132..3100
                     /locus_tag="ACG8KQ_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9105880.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8KQ_003053"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8KQ_003053"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9105881.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8KQ_003054"
     CDS             4130..4738
                     /locus_tag="ACG8KQ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9105882.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKCE010000297         4093 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000297 DAZKCE010000000
VERSION     DAZKCE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.165"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1521
                     /gene="msr(C)"
                     /locus_tag="ACG8KQ_003055"
     CDS             43..1521
                     /gene="msr(C)"
                     /locus_tag="ACG8KQ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9105883.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1568..2014
                     /locus_tag="ACG8KQ_003056"
     CDS             1568..2014
                     /locus_tag="ACG8KQ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9105884.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2213..2728)
                     /locus_tag="ACG8KQ_003057"
     CDS             complement(2213..2728)
                     /locus_tag="ACG8KQ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9105885.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2845..4047
                     /locus_tag="ACG8KQ_003058"
     CDS             2845..4047
                     /locus_tag="ACG8KQ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9105886.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1221 a          698 c          891 g         1283 t
ORIGIN      
        1 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
       61 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
      121 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
      181 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
      241 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
      301 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
      361 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
      421 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
      481 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
      541 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
      601 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
      661 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
      721 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
      781 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
      841 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
      901 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
      961 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
     1021 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
     1081 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
     1141 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
     1201 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
     1261 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
     1321 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
     1381 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
     1441 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
     1501 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
     1561 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
     1621 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
     1681 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
     1741 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
     1801 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
     1861 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
     1921 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
     1981 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
     2041 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
     2101 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
     2161 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
     2221 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
     2281 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
     2341 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
     2401 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
     2461 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
     2521 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
     2581 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
     2641 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
     2701 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
     2761 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
     2821 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
     2881 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
     2941 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
     3001 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
     3061 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
     3121 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
     3181 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
     3241 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
     3301 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
     3361 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
     3421 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
     3481 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
     3541 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
     3601 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
     3661 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
     3721 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
     3781 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
     3841 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
     3901 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
     3961 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
     4021 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
     4081 caagtcataa gaa
//
LOCUS       DAZKCE010000298         3884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.168, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000298 DAZKCE010000000
VERSION     DAZKCE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.168"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..1602
                     /gene="eat(A)"
                     /locus_tag="ACG8KQ_003059"
     CDS             100..1602
                     /gene="eat(A)"
                     /locus_tag="ACG8KQ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9105887.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1669..2196
                     /locus_tag="ACG8KQ_003060"
     CDS             1669..2196
                     /locus_tag="ACG8KQ_003060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9105888.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2407..3468
                     /locus_tag="ACG8KQ_003061"
     CDS             2407..3468
                     /locus_tag="ACG8KQ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9105889.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3471..>3884
                     /locus_tag="ACG8KQ_003062"
     CDS             3471..>3884
                     /locus_tag="ACG8KQ_003062"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9105890.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIV"
BASE COUNT         1352 a          662 c          856 g         1014 t
ORIGIN      
        1 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
       61 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
      121 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
      181 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
      241 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
      301 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
      361 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
      421 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
      481 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
      541 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
      601 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
      661 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
      721 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
      781 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
      841 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
      901 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
      961 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     1021 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     1081 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     1141 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     1201 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     1261 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     1321 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     1381 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     1441 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     1501 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     1561 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     1621 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     1681 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     1741 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     1801 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     1861 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     1921 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     1981 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     2041 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     2101 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     2161 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     2221 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     2281 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     2341 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     2401 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     2461 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     2521 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     2581 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     2641 ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat ggattatcat
     2701 atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt gaatgctagc
     2761 acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt ctcacagatt
     2821 gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga atggtatcag
     2881 aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt accaggagat
     2941 acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca tcgagggatc
     3001 catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt ttacatcgga
     3061 gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa agaacagaca
     3121 gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt agggaagcat
     3181 acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg ttttcgagag
     3241 attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat tactttagat
     3301 aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga tttgccagcg
     3361 gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt gatcaaccaa
     3421 atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa atgagcaaaa
     3481 tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga ttagatgaga
     3541 tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg gccgctgcgg
     3601 ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag tcctttaaaa
     3661 aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt gtgttagatg
     3721 ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt ctaattgctg
     3781 gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat gagctagggc
     3841 aagaagatct aatcaagctg aatttgacaa aaaatgatat agtt
//
LOCUS       DAZKCE010000299         2681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.191, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000299 DAZKCE010000000
VERSION     DAZKCE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.191"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..828
                     /locus_tag="ACG8KQ_003063"
     CDS             136..828
                     /locus_tag="ACG8KQ_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9105891.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1155..1652
                     /gene="dfrG"
                     /locus_tag="ACG8KQ_003064"
     CDS             1155..1652
                     /gene="dfrG"
                     /locus_tag="ACG8KQ_003064"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9105892.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1724..>2681)
                     /locus_tag="ACG8KQ_003065"
     CDS             complement(1724..>2681)
                     /locus_tag="ACG8KQ_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9105893.1"
                     /translation="SMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENR
                     NAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAF
                     YRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLA
                     SHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEP
                     NEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAIT
                     ILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          989 a          391 c          478 g          823 t
ORIGIN      
        1 ataaaaaagg aggattaaat gagtatttga tagtatttat attaaaatta catatgtaat
       61 ttaaaaacgc ttgacattgt gtttgagatc acttactttt agcatgtaaa agtattggat
      121 taaggagcta tgcaaatgaa aaagaaattt attactggtg ggattgtcgt tgcatttcta
      181 tgtctaattg gctacacagg cacgcggtac gcaaatgaaa ttattttgtg gggtggagag
      241 acagatattg atacaatcaa tgagaatctg acacagcttg atcatacatt gacagataaa
      301 gagcaagcga ttacagatct taccgctcga ctagcacaaa aagaacagac attgcaagat
      361 tcaaaggcag atgtccaaga ataccaaaca aaagtaaatg aattagaaaa ccaaaaaaat
      421 caatcggcag cagaaaaaca aaacttagaa agtcaattag tcagcaagga tgcacaacta
      481 caaagcaaac aaaatgaaat caatgtcaaa ctagatgaga tcaaccaaaa aaatgatgaa
      541 atcaatcatc tgaatgcaga ttggacagca agactacaag aagcacaagc aaaactttca
      601 gaattacaaa atactgtaaa tggtttgaat caacaaatcg caacactgac acaagaaaaa
      661 acaaatgtag aaactcagtt aacagataca caaaagaaat tagacgaagc aactgctgaa
      721 aactcaagct tgaaacaata tattcaaaag ttagaaaaag caaaagatga agtggaagat
      781 acagctgata aatctcagca aattgttgaa gaacatttgc gtaaataatc tttcttaaca
      841 agtaaaccta aattagtaaa gaagcaacag taatctatac taagtagttc agttttggag
      901 tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca
      961 aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt
     1021 gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca actaacgggc
     1081 aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa
     1141 aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg
     1201 gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta
     1261 caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac
     1321 ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa
     1381 ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt
     1441 tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa
     1501 aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga
     1561 atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc
     1621 atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta
     1681 aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta
     1741 tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct
     1801 ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg
     1861 caaattgagc atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag
     1921 ctgttgttaa tggtctttca agagatgata tataacgtct tatctgttga ataaagctat
     1981 tggtcgaatg gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag
     2041 ataaatccgt tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt
     2101 tgatagggtt atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt
     2161 ggaattgatg acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact
     2221 ttgttttgaa tcctctgata tctccccaat cgagcagttc tgcttttgct tgtccgaatt
     2281 cttttaagca ttgttttctg cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat
     2341 cgctcaacct taattctttc gtgaatactc tataaaaggc gtttctaata gagaagtcat
     2401 catcactaat cattcgccat tcgtcggcat taaccatttg tttaattaac caatagtgag
     2461 cagttgtagt ataaggagca tcaacattta aaccatcgac aaatctactc ctattagtaa
     2521 ttttccctaa ttcgtgaaga taagcattcc tattttcgct atcgttagcg gatggttctt
     2581 gtggataata agaccaatct aacctatcat ttttacgact aaaagtattg agatggtctt
     2641 ctttgaattt tcttgtatct tcatttaatt catccattga a
//
LOCUS       DAZKCE010000300         2562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000300 DAZKCE010000000
VERSION     DAZKCE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.193"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8KQ_003066"
     CDS             260..1171
                     /locus_tag="ACG8KQ_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9105894.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8KQ_003067"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8KQ_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9105895.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..>2562
                     /locus_tag="ACG8KQ_003068"
     CDS             2328..>2562
                     /locus_tag="ACG8KQ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9105896.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQ"
BASE COUNT          936 a          377 c          477 g          772 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca ag
//
LOCUS       DAZKCE010000301         1512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.228, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000301 DAZKCE010000000
VERSION     DAZKCE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.228"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..962
                     /locus_tag="ACG8KQ_003069"
     CDS             54..962
                     /locus_tag="ACG8KQ_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9105897.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            959..1439
                     /locus_tag="ACG8KQ_003070"
                     /pseudo
     CDS             959..1439
                     /locus_tag="ACG8KQ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          536 a          212 c          333 g          431 t
ORIGIN      
        1 tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat
       61 cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa
      121 ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt
      181 atgatattac atattttgta agtgatatag aaccgtttat atctaatgat gactggctta
      241 atcaatttgg gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg
      301 aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct
      361 tattgccctt ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa
      421 ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa
      481 gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt
      541 atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga
      601 ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta
      661 aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg
      721 agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc
      781 tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct
      841 atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg
      901 gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt
      961 gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga
     1021 ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact
     1081 ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc
     1141 agattgcctt gaatatattg acaatactga taagataata tatcttttat atagaagata
     1201 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     1261 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     1321 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     1381 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     1441 atgcaaggaa cagtgaattg gagttcgtct tgtggttctg ttgcaaagtt ttaaataaag
     1501 aataaaatcc ct
//
LOCUS       DAZKCE010000302         1088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.244, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000302 DAZKCE010000000
VERSION     DAZKCE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.244"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..734
                     /locus_tag="ACG8KQ_003071"
     CDS             87..734
                     /locus_tag="ACG8KQ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFP9105898.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          409 a          116 c          166 g          397 t
ORIGIN      
        1 tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct aaatataaaa
       61 atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta gaaaattggg
      121 atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc attactaaag
      181 aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa atttatccct
      241 ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga acaggaatta
      301 atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca gtttttaata
      361 agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc atttcttttt
      421 ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg tttcctaaaa
      481 aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat tttagttcat
      541 ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg ataggtaaat
      601 tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat cattctgtat
      661 gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt catagggtag
      721 atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac gtaagtgatc
      781 ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt tgattttgaa
      841 tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg cacaaaaact
      901 agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt ctagtgtgtt
      961 atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag caaaagattt
     1021 taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt taaatttcga
     1081 tagaaatt
//
LOCUS       DAZKCE010000303         1056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.246, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000303 DAZKCE010000000
VERSION     DAZKCE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.246"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..846
                     /gene="erm(B)"
                     /locus_tag="ACG8KQ_003072"
     CDS             109..846
                     /gene="erm(B)"
                     /locus_tag="ACG8KQ_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9105899.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          385 a          186 c          173 g          312 t
ORIGIN      
        1 cctaccagtg actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt
       61 ttaacttctg tatttgttta tagatgtaat cacttcagga gagattacat gaacaaaaat
      121 ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa
      181 ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg
      241 acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc
      301 aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt
      361 ctacagtttc aattccctaa caaacagagg tataaaattg ttgggaatat tccttaccat
      421 ttaagcacac aaattattaa aaaagtggtt tttgaaagcc gtgcgtctga catctatctg
      481 attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc
      541 ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct
      601 aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat
      661 aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa
      721 ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt
      781 accattactt atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg
      841 aaataattct atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg
      901 agataaatta ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc
      961 tacggggaat ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt
     1021 aggaaaatgt cctaagtgtg gcaacaatat tgtatt
//
LOCUS       DAZKCE010000304          865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4296
            SAMEA115707082-rid22572903.guided.261, whole genome shotgun
            sequence.
ACCESSION   DAZKCE010000304 DAZKCE010000000
VERSION     DAZKCE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707082
            Sequence Read Archive: ERR13196814
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287872.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287872.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,074
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 24 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707082-rid22572903.guided.261"
                     /isolate="VRE4296"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..350
                     /locus_tag="ACG8KQ_003073"
                     /pseudo
     CDS             1..350
                     /locus_tag="ACG8KQ_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(622..>865)
                     /locus_tag="ACG8KQ_003074"
     CDS             complement(622..>865)
                     /locus_tag="ACG8KQ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9105900.1"
                     /translation="SARPLIRFSLNGVNCLIASHSLNFLAEVDEGMKLVVTGYYNNRK
                     QFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          262 a          177 c          138 g          288 t
ORIGIN      
        1 ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat
       61 tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt
      121 cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag
      181 attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg
      241 tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac
      301 ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat
      361 gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga
      421 attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc
      481 atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct
      541 cgtgtgggtt caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt
      601 tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg
      661 gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct
      721 cacaacaaat tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc
      781 tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact
      841 gaaacggatc aacggtctgg cacta
//
